LOCUS       DAYYAG010000001        77714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000001 DAYYAG010000000
VERSION     DAYYAG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.001"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1093)
                     /locus_tag="ACHARQ_000001"
     CDS             complement(167..1093)
                     /locus_tag="ACHARQ_000001"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8408463.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(1178..1939)
                     /gene="pflA"
                     /locus_tag="ACHARQ_000002"
     CDS             complement(1178..1939)
                     /gene="pflA"
                     /locus_tag="ACHARQ_000002"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8408464.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(2183..4414)
                     /gene="pflB"
                     /locus_tag="ACHARQ_000003"
     CDS             complement(2183..4414)
                     /gene="pflB"
                     /locus_tag="ACHARQ_000003"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8408465.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(4691..7141)
                     /gene="parC"
                     /locus_tag="ACHARQ_000004"
     CDS             complement(4691..7141)
                     /gene="parC"
                     /locus_tag="ACHARQ_000004"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8408466.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(7153..9198)
                     /gene="parE"
                     /locus_tag="ACHARQ_000005"
     CDS             complement(7153..9198)
                     /gene="parE"
                     /locus_tag="ACHARQ_000005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8408467.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(9364..9798)
                     /locus_tag="ACHARQ_000006"
     CDS             complement(9364..9798)
                     /locus_tag="ACHARQ_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8408468.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            9925..10563
                     /gene="plsY"
                     /locus_tag="ACHARQ_000007"
     CDS             9925..10563
                     /gene="plsY"
                     /locus_tag="ACHARQ_000007"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8408469.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(10695..11570)
                     /locus_tag="ACHARQ_000008"
     CDS             complement(10695..11570)
                     /locus_tag="ACHARQ_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8408470.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(11652..12443)
                     /gene="codY"
                     /locus_tag="ACHARQ_000009"
     CDS             complement(11652..12443)
                     /gene="codY"
                     /locus_tag="ACHARQ_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8408471.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(12461..13861)
                     /gene="hslU"
                     /locus_tag="ACHARQ_000010"
     CDS             complement(12461..13861)
                     /gene="hslU"
                     /locus_tag="ACHARQ_000010"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8408472.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(13875..14423)
                     /gene="hslV"
                     /locus_tag="ACHARQ_000011"
     CDS             complement(13875..14423)
                     /gene="hslV"
                     /locus_tag="ACHARQ_000011"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8408473.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(14460..15365)
                     /gene="xerC"
                     /locus_tag="ACHARQ_000012"
     CDS             complement(14460..15365)
                     /gene="xerC"
                     /locus_tag="ACHARQ_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8408474.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(15563..16939)
                     /gene="trmFO"
                     /locus_tag="ACHARQ_000013"
     CDS             complement(15563..16939)
                     /gene="trmFO"
                     /locus_tag="ACHARQ_000013"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8408475.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(16908..18986)
                     /gene="topA"
                     /locus_tag="ACHARQ_000014"
     CDS             complement(16908..18986)
                     /gene="topA"
                     /locus_tag="ACHARQ_000014"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8408476.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(19108..19965)
                     /gene="dprA"
                     /locus_tag="ACHARQ_000015"
     CDS             complement(19108..19965)
                     /gene="dprA"
                     /locus_tag="ACHARQ_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8408477.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(20021..20788)
                     /locus_tag="ACHARQ_000016"
     CDS             complement(20021..20788)
                     /locus_tag="ACHARQ_000016"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8408478.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(20781..21641)
                     /gene="ylqF"
                     /locus_tag="ACHARQ_000017"
     CDS             complement(20781..21641)
                     /gene="ylqF"
                     /locus_tag="ACHARQ_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8408479.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            21901..22299
                     /locus_tag="ACHARQ_000018"
     CDS             21901..22299
                     /locus_tag="ACHARQ_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8408480.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            22536..23078
                     /locus_tag="ACHARQ_000019"
     CDS             22536..23078
                     /locus_tag="ACHARQ_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8408481.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(23161..23712)
                     /gene="lepB"
                     /locus_tag="ACHARQ_000020"
     CDS             complement(23161..23712)
                     /gene="lepB"
                     /locus_tag="ACHARQ_000020"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8408482.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(23864..24091)
                     /locus_tag="ACHARQ_000021"
     CDS             complement(23864..24091)
                     /locus_tag="ACHARQ_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8408483.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(24088..24606)
                     /gene="msrA"
                     /locus_tag="ACHARQ_000022"
     CDS             complement(24088..24606)
                     /gene="msrA"
                     /locus_tag="ACHARQ_000022"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8408484.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(24626..25339)
                     /locus_tag="ACHARQ_000023"
     CDS             complement(24626..25339)
                     /locus_tag="ACHARQ_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8408485.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(25342..26238)
                     /locus_tag="ACHARQ_000024"
     CDS             complement(25342..26238)
                     /locus_tag="ACHARQ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8408486.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(26396..27238)
                     /locus_tag="ACHARQ_000025"
     CDS             complement(26396..27238)
                     /locus_tag="ACHARQ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8408487.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            27370..28023
                     /locus_tag="ACHARQ_000026"
     CDS             27370..28023
                     /locus_tag="ACHARQ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8408488.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(28086..28613)
                     /locus_tag="ACHARQ_000027"
     CDS             complement(28086..28613)
                     /locus_tag="ACHARQ_000027"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8408489.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(28633..29580)
                     /locus_tag="ACHARQ_000028"
     CDS             complement(28633..29580)
                     /locus_tag="ACHARQ_000028"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8408490.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(29592..31475)
                     /locus_tag="ACHARQ_000029"
     CDS             complement(29592..31475)
                     /locus_tag="ACHARQ_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8408491.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(31638..33716)
                     /locus_tag="ACHARQ_000030"
     CDS             complement(31638..33716)
                     /locus_tag="ACHARQ_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8408492.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(33729..34004)
                     /locus_tag="ACHARQ_000031"
     CDS             complement(33729..34004)
                     /locus_tag="ACHARQ_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408493.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(34366..34821)
                     /locus_tag="ACHARQ_000032"
     CDS             complement(34366..34821)
                     /locus_tag="ACHARQ_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408494.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(34975..36183)
                     /locus_tag="ACHARQ_000033"
     CDS             complement(34975..36183)
                     /locus_tag="ACHARQ_000033"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8408495.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(36295..36627)
                     /locus_tag="ACHARQ_000034"
     CDS             complement(36295..36627)
                     /locus_tag="ACHARQ_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8408496.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            36775..37650
                     /locus_tag="ACHARQ_000035"
     CDS             36775..37650
                     /locus_tag="ACHARQ_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8408497.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(37732..38313)
                     /locus_tag="ACHARQ_000036"
     CDS             complement(37732..38313)
                     /locus_tag="ACHARQ_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8408498.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(38319..39578)
                     /locus_tag="ACHARQ_000037"
     CDS             complement(38319..39578)
                     /locus_tag="ACHARQ_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8408499.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(39580..40626)
                     /locus_tag="ACHARQ_000038"
     CDS             complement(39580..40626)
                     /locus_tag="ACHARQ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408500.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(40861..41136)
                     /locus_tag="ACHARQ_000039"
     CDS             complement(40861..41136)
                     /locus_tag="ACHARQ_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8408501.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(41379..42689)
                     /gene="der"
                     /locus_tag="ACHARQ_000040"
     CDS             complement(41379..42689)
                     /gene="der"
                     /locus_tag="ACHARQ_000040"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8408502.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(42871..44100)
                     /gene="rpsA"
                     /locus_tag="ACHARQ_000041"
     CDS             complement(42871..44100)
                     /gene="rpsA"
                     /locus_tag="ACHARQ_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8408503.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(44235..44915)
                     /gene="cmk"
                     /locus_tag="ACHARQ_000042"
     CDS             complement(44235..44915)
                     /gene="cmk"
                     /locus_tag="ACHARQ_000042"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8408504.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(44964..45599)
                     /locus_tag="ACHARQ_000043"
     CDS             complement(44964..45599)
                     /locus_tag="ACHARQ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8408505.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(45665..47068)
                     /locus_tag="ACHARQ_000044"
     CDS             complement(45665..47068)
                     /locus_tag="ACHARQ_000044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8408506.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(47055..48086)
                     /locus_tag="ACHARQ_000045"
     CDS             complement(47055..48086)
                     /locus_tag="ACHARQ_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8408507.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            48117..48338
                     /locus_tag="ACHARQ_000046"
     CDS             48117..48338
                     /locus_tag="ACHARQ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8408508.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(48503..49240)
                     /locus_tag="ACHARQ_000047"
     CDS             complement(48503..49240)
                     /locus_tag="ACHARQ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8408509.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(49393..50733)
                     /locus_tag="ACHARQ_000048"
     CDS             complement(49393..50733)
                     /locus_tag="ACHARQ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8408510.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(50970..52493)
                     /locus_tag="ACHARQ_000049"
     CDS             complement(50970..52493)
                     /locus_tag="ACHARQ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8408511.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(52871..53482)
                     /locus_tag="ACHARQ_000050"
     CDS             complement(52871..53482)
                     /locus_tag="ACHARQ_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8408512.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(53605..53730)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(53812..54528)
                     /locus_tag="ACHARQ_000051"
     CDS             complement(53812..54528)
                     /locus_tag="ACHARQ_000051"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8408513.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(54534..55103)
                     /gene="scpB"
                     /locus_tag="ACHARQ_000052"
     CDS             complement(54534..55103)
                     /gene="scpB"
                     /locus_tag="ACHARQ_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8408514.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(55087..55857)
                     /locus_tag="ACHARQ_000053"
     CDS             complement(55087..55857)
                     /locus_tag="ACHARQ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8408515.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(55854..56225)
                     /locus_tag="ACHARQ_000054"
     CDS             complement(55854..56225)
                     /locus_tag="ACHARQ_000054"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8408516.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(56244..57131)
                     /gene="xerD"
                     /locus_tag="ACHARQ_000055"
     CDS             complement(56244..57131)
                     /gene="xerD"
                     /locus_tag="ACHARQ_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8408517.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(57227..57700)
                     /locus_tag="ACHARQ_000056"
     CDS             complement(57227..57700)
                     /locus_tag="ACHARQ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8408518.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(57805..58668)
                     /locus_tag="ACHARQ_000057"
     CDS             complement(57805..58668)
                     /locus_tag="ACHARQ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8408519.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(58864..60963)
                     /locus_tag="ACHARQ_000058"
     CDS             complement(58864..60963)
                     /locus_tag="ACHARQ_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8408520.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(61183..62292)
                     /gene="rpoD"
                     /locus_tag="ACHARQ_000059"
     CDS             complement(61183..62292)
                     /gene="rpoD"
                     /locus_tag="ACHARQ_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8408521.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(62316..64157)
                     /gene="dnaG"
                     /locus_tag="ACHARQ_000060"
     CDS             complement(62316..64157)
                     /gene="dnaG"
                     /locus_tag="ACHARQ_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8408522.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(64339..66654)
                     /locus_tag="ACHARQ_000061"
     CDS             complement(64339..66654)
                     /locus_tag="ACHARQ_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8408523.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(66839..67699)
                     /locus_tag="ACHARQ_000062"
     CDS             complement(66839..67699)
                     /locus_tag="ACHARQ_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8408524.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(67758..68390)
                     /locus_tag="ACHARQ_000063"
                     /pseudo
     CDS             complement(67758..68390)
                     /locus_tag="ACHARQ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            68557..69318
                     /locus_tag="ACHARQ_000064"
     CDS             68557..69318
                     /locus_tag="ACHARQ_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8408525.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            69344..71935
                     /gene="mprF"
                     /locus_tag="ACHARQ_000065"
     CDS             69344..71935
                     /gene="mprF"
                     /locus_tag="ACHARQ_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8408526.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(72123..73310)
                     /gene="tuf"
                     /locus_tag="ACHARQ_000066"
     CDS             complement(72123..73310)
                     /gene="tuf"
                     /locus_tag="ACHARQ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8408527.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            73678..74034
                     /locus_tag="ACHARQ_000067"
     CDS             73678..74034
                     /locus_tag="ACHARQ_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8408528.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(74275..75279)
                     /locus_tag="ACHARQ_000068"
     CDS             complement(74275..75279)
                     /locus_tag="ACHARQ_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8408529.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            75445..76296
                     /locus_tag="ACHARQ_000069"
     CDS             75445..76296
                     /locus_tag="ACHARQ_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8408530.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            76313..76558
                     /locus_tag="ACHARQ_000070"
     CDS             76313..76558
                     /locus_tag="ACHARQ_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408531.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            76745..77449
                     /locus_tag="ACHARQ_000071"
     CDS             76745..77449
                     /locus_tag="ACHARQ_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408532.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        21388 a        16363 c        13389 g        26574 t
ORIGIN      
        1 tatccgtttt attttactta aaaggctttt acgtaaaaag gaggaagaac aagagtcata
       61 attgactgtt attctcctcc atttcttcgg cctttagtat gttctatgtt tctttttata
      121 tcaatcgctt atccattcat tttgaatgct tcatactacc atttgattag ctaaatacag
      181 ccgtcaattg aggtacgact tgttttttac gtgaaacgac acctttaagc aaagcacggt
      241 tgttggtcaa agttgtttgg aaagcttctt ctactttttc ttttccttta ccagcaacaa
      301 gtaattctga atcactgtct aagatgtttg tgacaactaa tacgaacaga tcgtagccat
      361 ttgctttcat ttcatcttcc attgctgctt ctagctctgc ttgtcgatcc atcacttcat
      421 taaggtccac tgtattgact tgagcgatac gaacgttgga actgcccatt gggaaacttt
      481 ttgcatccaa atcaagaaga accgcagctg atttatcact tagatttgtt cctgctttca
      541 acatttcaag tccataagtg tttagatcga cttcagcgat tgctgcaagt tcttttgcag
      601 ctgcttcatc ttcaggcgta catgttggtg atttgaacaa caatgtatct gaaatgatag
      661 ctgagagcat gattcctgcg atttctttag gaattgttac ttggttttct ttatatagtt
      721 ttaaaataat cgtacttgta catccaactg gttctgcacg atagtacaat ggattagctg
      781 tttcaaagtt tgcaatacga tggtggtcta ctacagctaa aatatttact tcgctgatat
      841 ctgaaatact ttgttggaat tcattatgat ccacaagcat gacatctgca ttttctgctt
      901 tagctgactc tactactcgt ggtgcagata gctgaaagtg gtctaatgcg tattgtgttt
      961 cttcattagg tgtacctaga gcaactgctt ctgtttcttt acctaattca ttttgcaagt
     1021 gtgaaaacgc aatggctgcc ccgattgcat ctgtatctgg attttggtga ccaaaaacta
     1081 aaatttttga catgtcattc gctcctttgt gatttctcct atttaataat agcaaaaaaa
     1141 cggaaagaag caatacttat cttctttccg atgcgaccta tcgcgttaaa tattttgtat
     1201 actcttctgt atgaagcaat cgtttagcat tttcgacacg ttcttttgta ggtggttcga
     1261 tcccttctaa tggatatttc aaaccaagtg tttcccactt gtattttccc atcgtatgat
     1321 aaggaagaat ctctactctg tctacatttg acaaggtttg aataaagttg tctagtcgga
     1381 tcaaatattc atcataatca cttcgctcag gaactagtac atggcgaatc caaaccggtt
     1441 tgttgatttt tgacaaatat tgagccattt ctaaaatatt ttcattgtcc caatgcgtta
     1501 gttttttatg ctcttcatta tcgatgtgtt tgatgtcaaa taacagtaaa tctgtatact
     1561 tcatcaattc ttgaaaacgt gaaaagaaag gttcttcata cgtaaaaggt tttccgcatg
     1621 tatccaatgt agtatgcatc ttccgctctt ttgctttttt aaatagttcg atcaaaaaat
     1681 cgatctgcaa tagaggttct ccaccactaa cagtaatacc acctttatct ccccagtaag
     1741 gtctgaagcg ttctgcttta tctaaaagtt catctgctgt gtattctttc cctccgccaa
     1801 tattccaagt atctggattg tgacaaaatt ggcaacgcat gcggcatcct tgcataaaca
     1861 cgacaaagcg cagtccaggt ccgtctactg aaccgaaagt ttcgatagaa tgaacatagc
     1921 cgattgtttt ttcttccatt taagttcaca tcttttcttt gatttcattt taagtacaag
     1981 ttagctgtcg aagcgaagct ttctgaacaa aatttttcgc aattgccgct tcgttatgtg
     2041 taaaatttac agggacggaa catacaagtc ctgcccctgt aaatcgtttc tttcaaataa
     2101 gcatactgat tattcgattt tgatgcgatg gctattcaac gtcctttcga cgctaagtcg
     2161 aaaaactttt ttcacgaact ttttacattc tgtcgtgtga agttctcatc aagacatcta
     2221 attgttgttc tcttgtcaag tcacggaatt taactgcata accagatacc cggatcgtca
     2281 agttcggata tttcattgga ttttccattg catcacgcaa taaatcatta ctgaatacat
     2341 tgacattcaa atgataagct ccttttgaga agtatccatc taagacatta cgcaagtttt
     2401 cgattcttgt atcttgttct tttccaagac ctgaaggatt gattgtttgg gtatttgaaa
     2461 ttccatccaa tgcacagccg taattcattt tagctgttga gttcaggcta gctaataacc
     2521 cactttgttc cgcattgtaa cttggatttg ctcctggcgc aagtggtttt ccagcttcac
     2581 ggccatcagg agtatttcct gtatttttac cgtaaacaac atttgaagta atcgtcaata
     2641 atgaagttgt tggttttgag ttgcggtaag ttttatgacg tttgatttgt cccatgaagt
     2701 attccaagat ccatgaaact aattcatctg cacggtcatc atcatttccg tatttaggga
     2761 agtcgccatc cacttcaaaa tcaactgcta ttcctttttc attacggatt gcttttactt
     2821 ttgcatattt gatcgctgac aagctgtctg ccgcatgaga gatcccagca atacctgtag
     2881 caaatgtgcg ttgcaagtca gaatccatca aagccagctg agctgcttca taagaatatt
     2941 tatcatgcat ataatgaatg atgttcaacg tattgacata tagttctgct aaccaatcca
     3001 tcatattcat gaagttatgt ttcacttctt caaagtctag tatttctgat gtgattggac
     3061 gatattctgg tccaacttgg gcgccagtca cttcatcgac tccgccattg atggcatata
     3121 atagtgtttt agccaagtta gcacgagcgc caaagaattg catgtctttt ccgacaactg
     3181 ttcctgatac acagcaagca atcgcacaat catcagaacc ccattgttca cgaagctgat
     3241 tatcattttc gaattgaatc gaagaacttt cgatagcgat tctgctagca tatgttctga
     3301 atccttctgg aaggtgttca gaatacaaaa cagtcaagtt tggttctggt gaaggtccca
     3361 tatttgttaa tgtatgaagc agacggaaat cattctttgt aactaatgaa cgaccatcta
     3421 atcccatacc tgcaatggat aaagtagccc agatagggaa tcctgagaat aattggttat
     3481 attcaggtgt acgcgcgaat ttgaccatac gtaatttcat tactaagtgg tcgatcaatt
     3541 cttgtgcttc tttttctgtg attactccat tatccaaatc acgttggata tagatatcaa
     3601 ggaatgctga gatacgtccg attgacatcg cagcaccatt ttgtgatttg attgctgcta
     3661 aatatccgaa gtatagccat tggacagctt cttttgcatt tgcagctggt ttagaaatat
     3721 catagccata tttcgcagcc atttctttca tttgcgctaa tgcacgatat tgttcagtga
     3781 tttcttcacg taaacgaatc acttcttcac tcattgtatg ataaccagtc atatcatgat
     3841 cttttttctt ttgttccatc aaatagtcga ttccatacaa tgccacacga cgatagtcac
     3901 cgatgatacg tccgcgaccg taagcgtctg gaagccctgt aatgattttg tttttacgag
     3961 cagcacgcat ttcagcagta taagcgtcaa atacaccttg attatgtgtt ttgcgccagt
     4021 cagtgaaaat atgtgtcaat tcatcgtttg gtgtatagcc attgcttttt agcgcattgt
     4081 tagccatatt gattccgccg aacggcatga atgcttgttt caaaggtttg tctgtctgta
     4141 gaccgacgat tttttccaaa tctttatcca aatagcctgc atcgtgtgaa gtaatagttg
     4201 atacgatttc tgtgtccata tcatagacac cgttacgctc gaattggata tcatttaact
     4261 tttgcaattc atcccataat ttttctgttg cttctgttgg gccttccaaa aagctgtcat
     4321 caccgcgata ttcagtgtag ttattttgaa taaagtctct agtgtctacg ccttctttcc
     4381 atttggtacc tttaaatcca ttccattgct ccattgtatg gacctcctaa atttctgttt
     4441 tatgtaacag tttttacgtg attagtataa cacagcaccc tatctttgca agggttttct
     4501 tgttttttat ttaacaattt ttatgtttta tttacatata tttaaatcaa cgttgacata
     4561 ccaacattta agcattgtga acactttcat ctttttttgc aaacgtataa ggatttatta
     4621 aaaatgaatg aaaaagagac gccacctgta ttaatacaga tggcgtctct tcattaaaat
     4681 actacaacag ttacgactcg atgaaaatct caggagcatc gataacttcg atgacttctc
     4741 cacctttttt ctcatccatc gcaaatgatc cattcgatgt tcgatcacct atagggaaat
     4801 cagttgggtc aatcgttaac tgctgccctt tttgattcat caaggttatt tcttttgatc
     4861 ttccttccga catatagata atacggtgcg gatttttttt cagctcacgc atgaccgtca
     4921 ctccccgctt cgcacgtgaa gtttggctga tttcttgagc aagcatgcgt ttaacagctc
     4981 ctcgctgagt aacgattacg atcggcgtat caccttctgc taacacaagc agcccgttga
     5041 taatcctatc tccatctttc aaattcatag acttcactcc agctgccttt gctccaacaa
     5101 cgggaacatc tgttaaagga tagcgcaaac ccattccttg atgcgttact aagaaaacat
     5161 ctaagcctgg ctgatcttct ttcagataca cagcaatcag ttcatcttct tccgccttta
     5221 gcttcataca gctgagcgga cggctcttat aggtgcgcca tggttcgaac tctgtcattc
     5281 gtgtttgctt gatcaatcca ttcttgctga tgaatacaaa tgttttctct gcttgaattt
     5341 tttgatatgg gaaaacagct aaaatcgatt cgtctacaga cagattggag attgtttgag
     5401 aaatgtgctc accagcatct ttccatttca aatcgggtag ttcatggaca gggcggtaga
     5461 tcacatttgc tttatttgtg atcagaagaa catggtccaa tgtactcaat tcacctgagt
     5521 atagcaagta gtcgccttct cgcatcccga tttcttctgg tttggaagca ctataagaac
     5581 gaaggcttgt tcgcttcaca tagccttctc gcgtcacact gacaattacg tcttcttgtg
     5641 ccactaagac ttgcgtatca attttgattt cttcgatttc gtcttcgatt gtcgttagac
     5701 gagcggaaga ataatttttt ttcacttctc gcaattcttt tttcataaca gagaaaagct
     5761 ctttgtcatt tgaaaggatt ttattcaact ctgtgatttg cgcaatcaag ctttcggatt
     5821 ccttttgaag ctctgtgata tccgtgttcg tcaaacggta taattgcaat gtgacaattg
     5881 cttctgcttg ttcttctgta aattgaaaga cgtcgactaa attctttttg gcgtcttttt
     5941 tgtctttact ttcacgaatc gttgcgatta cttcatccag aatcgataaa gctttcatca
     6001 atccttctac gatatgctgt cttttttgag ctttggctaa atcgaattga ctacgttttg
     6061 ttatgacttg ctttcgatgc tcgatataac ttcttaaaat gtctttcagt ccaacttgat
     6121 gcggagtcat atggtcgata gcgaccatat tgaaattgta attgatctgt agctcagtgt
     6181 ttttgaacaa ataattcaaa attccttgag cattggcgtc ttttttcaat tcgacgacaa
     6241 tttgcagacc tgttctatca ctttcatctc gtacttcagc tattccatcg attttcttat
     6301 tcaaacggat ctcatccatt tttttcacca aggtcgcttt atttacttca tatgggattt
     6361 ctgtgatcac gatttgctgt ttattaccct tcattgcctc tatctttgtt ttcgaacgaa
     6421 gaataacttt tcctcgtcct gtttcataag cttttttgat ttcatctttt ccttgcaaga
     6481 tccctcctgt tggaaagtca ggtccaggaa tatattccat caatttttca aggctcgctt
     6541 gtggatgatc gatcatatag attgtgccat cgatcacttc agctaagtta tgagtcggga
     6601 tttctgttgc atatccagca gatatacctg tcgatccatt gacgagaagg tttgggtatc
     6661 gagctggaag gactgttggt tctttttctg tatcatcaaa attccacacg agatcgactg
     6721 tttctttctc gatatctgct agcatctctc cacttaactt ggataatctg gcttcagtat
     6781 aacgcatcgc agcaggtgga tcaccgtcca tactcccgtt attcccgtgc atttcgatca
     6841 gtacttcccg caacttccag tcttggctca tgcggaccat cgcttcataa atactgatgt
     6901 caccatgagg atgataattc cccatgatat ttccgacaga ttttgctgat ttacggaatc
     6961 ctttgtcata cgtattgcca tctttgttca tggcaaataa tatccggcgt tgcactggct
     7021 tcaatccatc acggatatca ggcagtgcac gttcttggat aatatatttg gaataacgtc
     7081 cgaagcggtc acccatcact tcttctaaag ttaattcttg tacttcttgt cgattttcca
     7141 tcaggtgacc tcctattcta cttcaaataa ctgattgttt tcatttttat ctgctcgttc
     7201 ttcatctaat aaatcattag aaactgaagg agagatttca gtatcctctt ttttatctaa
     7261 gatactgcca tcttcttcga gcgtaaattg cacgtgattt tcgatccatt ttcttcgagg
     7321 ttctactttg tcacccatca gtgtggttac tcgacgttca gcttgcgcag catcatcgat
     7381 acggacgcgg atcaacgtac gggaagtcgg atccatcgtt gtttcccaca attgttccgc
     7441 attcatctct ccaagtcctt tatatcgctg cagcatatat cctttgccga cttttttaat
     7501 cattgctgcc aattcgtcat ctgtccacgc atattcaatg acttgcttct ttccttgtcc
     7561 tttggatact ttatataaag gcggtaacgc gatatatact ttcccagctt caatcagcgg
     7621 tttcatataa cgataaaaga aagtaagcag taatacttga atatgggcgc cgtctgtatc
     7681 cgcatcggtc atgatgatta ctttgtcgta attacagtct tcgatagaaa actcaggacc
     7741 gactcctgca ccaatcgtat agatcatcgt attgatttct tcatttttca agatatcttg
     7801 cattttagct ttttcggtat tgatgacttt tcctcgtaaa ggtagaattg cttggaactt
     7861 tctgtctctt ccttgtttag cagatccgcc agcagaatcc ccttcgacta aatacaattc
     7921 atttttctta gggtttcggg attgtgcggg tgtcaatttt cctgaaagaa gagattcgcc
     7981 ttttttacgc ttcttcccat ttcggctttc ttctctggct ttacgtgctg cttctctcgc
     8041 ttctctggct ttgattgctt tacggaccaa catctggctc atttcgctat tttcctgcaa
     8101 ataaaagccc atttgttcac tgatgacgtt atctactacg gtacgagcaa ctggcgtacc
     8161 aagtttttct tttgtttgcc cttcaaattg cagaagattt tcaggtacac ggatagaaag
     8221 tacagcagct aacccttctc tgaaatcact accttccaga ttcttgtccc gttctttcag
     8281 caaaccaact tttctcgcat attcattata agatttcgtc atcgccgctt tcataccagc
     8341 ttcatgggtt cccccatctt ttgtccgaac gttattgaca aaagataaaa cattttctga
     8401 ataaccgtca ttgtattgat aagcgacctc tacttcgata ccttcttttt ctcctgaaaa
     8461 ataaacaact ggtgtcaatg tatctttttc ttcattcaga taatcaacaa attctttgat
     8521 tccttcttcg taatgaaaaa tttccttgac tggttcttct ccacgaagat cagaaagaga
     8581 gattttaacg ccttttagaa gaaaggcaga ttctcttaac cgttcggcta aaatttcata
     8641 tgaaaatttc gttgtactga atattgtatc atctggtaaa aaatggacag tcgtcccgtt
     8701 tgcttttttt gtttttccta tctttttcaa agtaccgtca ggtttaccgc cgtttttaaa
     8761 tttctgctga tactctacgc catctctaac gatcgttaca attagccatt tcgataaggc
     8821 atttaccaca ctggctccta caccgtgcag tccaccagaa gttttgtacc ctccttgacc
     8881 aaactttccg ccagcatgca gcacagtaaa aatcacttca actgttggta ttccggaagc
     8941 atgcattcct acaggcatcc cacgtccgct atctgcaaca gttatactat tgtcttcatg
     9001 gatggtcaca tcaatctcac tgccataacc tgataatgct tcgtcaactg cattatcgac
     9061 gatttcatag actaaatgat gcaagcctcg gctgtcagtg gatccaatgt acatccctgg
     9121 tctttttcta actgcctcta atccttccaa tacttggatg gacgagtcat tgtattcgtt
     9181 gtttactttt tttgccaagg caaacgctcc ttcaataata ctttgcggca aaaccgcacc
     9241 acgattatca tactagaaaa ggtcgtgaaa aaaaagaggg tagacgagaa atcaatttta
     9301 attatctcct aaccgctttt tcgacattcc cctctatcga tcatctcttc tttgtggtca
     9361 tttttatttt tggttttctt tcaacttacc aagttcaatc ttaatacaac gatccattac
     9421 gatatctgta cgtcctgctt ttcttagcat ctcagctgct tctttgtttt caatccctaa
     9481 ttgtgcccag aacactttag catcagtttt caaaaaatca cctgctattt caggtaaaaa
     9541 ttcactccgt ctaaatacat ctacgatatc gattggatca tcgatttctg tcaaattcgc
     9601 atagactttc tcgcctagaa tttcttcacc agccaataat ggatttactg gtatgatttt
     9661 atatccattg tcttgcaata cctgtgcaac catgtaactt gttcgttctg gtttattgct
     9721 caagcctacg acggcaatat tttttgcttc ttccaaatag ttgaaaatcg tttcttgtgt
     9781 tggatttttt acagacattt ctgccacccc ttgcttttct ttttattcca tctattatac
     9841 ttgtatcttt cattttttca attgttcctg ttacctattc atgctaaaat aaaggatagt
     9901 tttcaacttg aaagagggta aaaaatgaaa ttgatcttat tacttatttt agcatatctt
     9961 ttaggttcta ttccttccgg tgtctggatt ggcaaattgt ttttcaaaaa agatatccga
    10021 caacacggga gcggtaatac tggaacgacc aatacatttc gtgtattagg caaaacagcc
    10081 gggattgttg tcctgcttat ggatatttta aaaggtacat tggctacttg tttgccgatg
    10141 atcttccacc tttctgggat taaccctctt tggtttgggg tatgcgccat tcttggccat
    10201 acatttccga tttttgctgg gttcaaagga ggaaaagctg ttgcgacaag tgcaggaatg
    10261 cttttaggat ttaatcctgt attttttgtt tattcttgta tcatctttat cgttagttta
    10321 ttttgtacaa gtatggtgag tttgagcagt atgatcagtg cagtattgat cactttatct
    10381 acgatcgtcc ttccttatgt agctcctgtg attttggcta agcccaattg gttactgacg
    10441 atcattgcct ttttagtttc ttcctttatc ttttatcggc ataaagacaa tatcaagcgt
    10501 atcagaaacc atactgaaag tcgaatcccg tttggtttga atagcagcaa gaaaaaatcc
    10561 tgatactttt tcattccaaa gagataaaac gggagtgtga caaatcgatg tcacactccc
    10621 ttattactta caggatagct tttttacaca ccttggtaaa cggtgttccc tgctaacttc
    10681 tcggtcacaa cctcttactt cactgtgatg gaatattttg tcttgaagat ttcatgggca
    10741 cctagttgat tgatccctac tttttccatc aagtttccac tactgttcac cgtgtcagcg
    10801 attccgcacc acggttcgat acaaacgaaa ggcgcttctt ttggataagg agaccagatc
    10861 cctacataag gcatatcttt atagcttaaa gttacgctat gggaagattt ttcacttcga
    10921 atggcgtacg catttagacc ccttgtttca aaaaccaagg catcttgctc aaaaagttca
    10981 cgattcaaag ccagattcgt atttgtctga cctaacgtct tttgatccat atcgatgaat
    11041 gggcctttca atggtaggcg taatcttgat ttcaatggag aaaatgccag atagaaatct
    11101 tcaaatgaca atccttcttc aagaggtaca ttgaaagcag gatgtccacc gatagaaaag
    11161 aacatttctt tttcaccggt atttgccact tgataatgaa ccgtgatgcc gtctcctcct
    11221 aattcatagc tgatcactaa ttcaaaatca aatggatact tttctaatgt ctccggcgta
    11281 ctccttaaga tgaaactagc catttcttgt ccatgttcaa tgacttcaaa atccatatcc
    11341 cgagcaaatc cgtgttgccc catttgatat gttttccctt gataaacata ctgatcatct
    11401 tttaaacgtc caacaaaagg gaataaaacc ggtgcatgtc tgccccaata cgctggatca
    11461 ccttgccaga tatactctaa accatgttct tttgatttca gactgatcaa ttccgcaccg
    11521 tgttcagcga tttcggcgat cagctggtca ttttgaataa taacagtcat tgccattcct
    11581 ccaacttcga cttacttttt aaaatgggaa ggctggaaca aacatttcta tctgccccag
    11641 tccccttact tctaataact gtgttttgct aactcgtctt taaaacggga attcaatact
    11701 tttaaataag ttcccttcat gcctaacgaa cgagattcaa tgattccagc tgattccaat
    11761 tttcgtaaag cattgacgat cacagaacga gtgatgccga tactatcggc aatattagaa
    11821 gcagtcagac gaccttcatc tccatccaat gcttcaaaga tagcttgtac agccttcaat
    11881 tcgctataag ataatgtgtt gattgccatt tggacagccg ttgcactccg tacatctgct
    11941 tcgatgcttc gagacttctg ataaaggatc tgcataccga caaccgtagc tccatattct
    12001 gctaagacta gatcgtcatc tgcaaacgat tgttccatac gagataagat aatcgtaccc
    12061 aaacgttcgc cagctccaaa gatcggaacg acagtcgtca gaccaaatgg atatttctct
    12121 cttgactcta ccggaaatgc agtaatatca ctagtaattg ggatattagc ttcagttttc
    12181 atcaactgtg ccactgtatc agtataagat tcaggaaatt gtttctcaac aaacattgtt
    12241 ttgattcgtt cattgttcac atcgtgacgt tcattataac ctagtaatac tccgtcactt
    12301 cctatgatat aggtgttgct gtcaagaata ccacctagtg tgacagccat tttatcgtaa
    12361 ggcaaatctg cttgcatgtc aaaagtattc ttttgctgca aaagtgtgtt tatcttgcgg
    12421 gttttcgtta gcaagtctgt cattgtcgtc ctccgatttt ttataaaata tagcgactta
    12481 aatcttcatt tgccgcaatt gagccaagtt tgtcatccac atatgcttct gtgatcgtta
    12541 tttctcccat ttgcatatct ggtgcttcaa ataatagatc ttctagtagt ttttccaaaa
    12601 tggtatgcag tctgcgtgcg ccgatattat ctgtgtcacg gttgacatcg aaagcgattt
    12661 gagcgatccg ttcaattgct tcttttgtaa acgtaactga cacattttct gtaccgatca
    12721 acgcgacgta ttgttttact aaagcattat ttggttcggt cagaatcttc acaaaatctt
    12781 cagctgtcaa atcatctaat tctacccgta tcgggaagcg tccttgaagt tctggaatta
    12841 gatcacttgg tttagacaaa tggaaagctc ctgaagcaat gaataggacg tgatctgttt
    12901 ggatgatccc gtattttgtg tttacttgtg aaccttcgac aattggcaat atgtctcttt
    12961 ggacgccttc tctggatact tctccagatt gctgtgattt agaagtgatc ttatcgattt
    13021 catcgataaa aataattcca ctttcttccg ctaatttgat tgcctcacta tggatatcag
    13081 catccttgac taattttgct gattcttcct tgatcaataa ctctaaagct tctttgactg
    13141 ttaccgtccg ttccactttt ttcttcggtg tcaaagcacc tagagtttca tttaggtcga
    13201 tccccatttg ctccaatcca ttgttcatca ttggtgcagg tttcttttgg tcttcaactt
    13261 caatggttac ttcacgacta tccagtaaac ccttttgtag ttgttccaag actgtacggc
    13321 gattgattcg aatatcttct gtcacttctt cttgcggttc agcggtattt tgctgactag
    13381 cattaaaaag attcatcatt tgttcaaatt gattagtgga ttgtttctgt tcttttttga
    13441 taccaggtac caagattttt accaaacgtt tttctgcatt ttttttagca cttgtgtata
    13501 cacggctgta ctgctctttt tctacgattt ggatcgcatt ttctactaaa tcccgaacca
    13561 ttgattctac atctcttccg acataaccga cttcagtaaa ttttgttgct tcgactttga
    13621 caaatggtgc attgacgatt tttgccaaac gtcgtgcgat ttctgtttta ccgacccctg
    13681 taggtccgat catcagcata ttttttggtg taatatcttg ttgcattttt tcatctaact
    13741 gcaaacggcg atagcggtta cgcaaagcaa cggcaacaga tttttttgct gcgtgctgtc
    13801 ctacgatata ttcatctaat tcacttacga tttgtcttgg tgtttgtttt aattcataca
    13861 tatctactca cctcttacag ttcttctacg ataatattat gattagtaaa gatacagata
    13921 tctgcggcaa tatttaacgc attttctgca atttctttcg cactcatgtc gttttgagca
    13981 tagtttttca ttgctcgggc tgcagccaac gcaaaattcc ctcctgatcc gatagctaag
    14041 ataccatcat ctggtgtgat aacttctcct gtacctgaaa caagaagcat ttctttatca
    14101 ttcattacga tcagcatcgc ttcaagtttt tgcatagatt gctgtgtccg ccattcttga
    14161 gctaattcga cagctgcgcg ttgtagattc ccgttatatt cgtttagttt gccttcaaat
    14221 ttttcttcta aagtaaacgc atctgcaaca cttccggcga aacctacaac aacttctcca
    14281 ttgtagatac gtcgtacttt ctttgcagtg cctttcatga caacagattc acccattgtt
    14341 acttggccat ctccagccat agcaaatttc ccgtcttttt cgacggcaca gatagtggtt
    14401 gaatgaaatg gtgattcgac catgtttgtt tcctcctaaa tttgttcttt tccttatttt
    14461 tacgctcttg gatggaaaga acgatagttt ttttgtaaac tttcttttgt tacatgggca
    14521 tagatctggg tagtcgataa attggcatgt cctagcaatt cttggactgt ccgcaaatct
    14581 gccccattat ttaataaatg agtagcaaat gtatggcgca gcatgtgagg atggatatca
    14641 ctatccaaac tgcttttttt gatcatttga ttcaagacat attcaattcc agtaggcgtg
    14701 attggttttc catgatgatt cacaaaaacg atatcatgct cttcgtgata ttttgtcatc
    14761 aatacctggc ggccttccct gaaatattct tgcagagcat cttgagcaaa agaacctaga
    14821 ggagcatatc tctctttgtt ccctttacca tggatcaaca tgacctctga atcaaaatca
    14881 atgtctgata ccgacaaact gctacactca cttaaacgga tgcctgaccc gtaaaggact
    14941 tccagtaaag cgcggttcct caaatccaaa ggactatctc ctgctacgct gtcaaaaagt
    15001 gcttgcattt cattttcata aaaaaaacgt ggcagtctca aatttttctt tttgagatga
    15061 acgtaagaaa atggattttc ttttatcact tcttctttga gaagatactg ataaaacgaa
    15121 cgtaggctgg ctatcttgcg actgatagaa tttctgctat agctaggacg atgatcagct
    15181 aatgcactca aatagacacg aaccgtaaaa tattccgcct tcagcagatc agcgtctcca
    15241 gattcctcca aaaaatgttt gaaatggaga atgtcttctt catatgcttc tttcgttttt
    15301 tctgaatatc ctctttcgac aatcaaataa cgcaaaaatt tttgttccca cgtggcttct
    15361 gtcattttct caccttcctt ttgtcccaga gaataattta gcacagcgtt tataaaaaga
    15421 caaataaatt gtcagaattt aaccaatatt tctaagtttg ttcacaattg gcgcaattcc
    15481 tcacattttg tttgagaaat tcttaagaaa aacgcaagaa aaagagcgag actatccgtc
    15541 tcaccctctt catggagttt ccttactcat ttgggattgc agttttttct aattctgttt
    15601 ttattgcctc attagctttt aaagctcgtt gtgcaattgc ttcatatctt gcttttttgt
    15661 ctcgaatgcg ctctggcagt tcaggaaaca gaccatagtt ggcattcatc ggttggaaat
    15721 gttttccttc cgcatgagta atgtagtaag ccatacttcc aatagctgtt tcttgtggaa
    15781 actcgactgg ttccatttct tttgctaagc gtgcggcgtt gattccagca agtagtccgc
    15841 tcgctgcact ttcaacatag ccttctacac cagtcatctg acctgcaaag aacagatctt
    15901 cgcgtttcgt tgattggtaa gtcggtttca gcaattctgg cgaattcata aagctgtttc
    15961 ggtgcatcac accatagcga acgaattcag cattttctaa cccaggaatc atccggaaaa
    16021 cacgtttttg ctcgccccat ttcaagtgag tctgaaagcc cacgatgttg tataaagaag
    16081 ctgcagcatt gtcttgacgc agctggataa cagcataagg acgtttgcca gttttaggat
    16141 cttccaatcc aactggtttc ataggtccga aaagcatcgt tttgaatcct cgctgtgcca
    16201 ttacttcaat tggcatacat ccttcaaaat atttttcttt ctcaaaagat ttaagttctg
    16261 ccacttcggc attgattagt gcctcatgga aagctttgaa ttcctcttct gtcatcggac
    16321 agttcaagta agctgcttct cctttgtcat agcgggactt caaatatact ttatccatat
    16381 cgatcgtagc tttgtctacg attggtgcag ctgcatcata gaaataaaaa ccatctgaac
    16441 cattgaatgc tttgatgctt tctgctaatg gatgagaagt caaaggtcct gttgctacta
    16501 cggtgattcc ttccggtatt tcagtaattt cttcgtttat cactgttact aatggatgct
    16561 ctttgacttt tcgggtaatt gtttcagaaa aagaatcgcg gtcaacagct aaggcaccac
    16621 cagctggcac tgctgtttca tctgccgaag tgattactac cgaattcaat cggcgcattt
    16681 cttctttcaa taaccctact gcattagcca aactgtttcc tcttaatgaa ttggaacata
    16741 ctagttcagc aaaattactt gtgtgatgcg cctcagtcga tttgactgga cgcatttcat
    16801 ataaacggac agggactcct gcttgtgcga tctgccaagc agcttcactt ccggcaagcc
    16861 ctgctccaac aacatttacc attttcaatg aaagaacctc ttttctatta tttttggatg
    16921 tcttcttcgt aatctccatt tacacagact acttgtttac cgcctttgac tttcttctcg
    16981 accagatatc cgtcacattt tggacaattt ctgcccactg gtttatccca agaagtgaat
    17041 tcacattctg ggaaacggga acaaccatag aatagtctgt ttttcttaga ttttcgttca
    17101 atgacttgtc cttcatgaca tactgggcaa gtcacaccga tttcttttac gattggtttc
    17161 gtattccgac aatcaggaaa attactgcaa gcataaaact tcccatattt ccctaatttt
    17221 atcaccatcg gatgtccaca gacatcacaa tcgaatcctg ctggttcatc tttgatttga
    17281 attttttcga ttttttcttc cgcattcgtc agttcttttt caaacggttt atagaaacga
    17341 tcaaccactt tgacccacgc ttccgtgcct tcttcaattt tatccaaatc tccttccatt
    17401 tctgctgtga aatggatatc gacgatttga gggaagaatt cacagattaa tgaattgaca
    17461 atttctccta gttcagtcgg ctcaaatctt ttttgtgtca attttacata gtagcgtctt
    17521 tggatcgtat caagtgttgg tgcataagtg gaaggtcgcc ctaccccgtt ttcttctagt
    17581 gcacgaatca acgtagcctc actaaaacgt gctggtggtt gagtaaaatg ttgttttggt
    17641 tcaatatcaa cagattggac tacatcgcct tcttcaagtt ctggaagaat attttctttc
    17701 tcttctttgc cgtcatctct accttccaca tacacttgca tgaatccttt gaatttgatt
    17761 tttgaaccgt tcgcaatgaa gatgacacca ttttgttcta atgtcacttt cattgtatct
    17821 aaaatagctg gtgtcatctg actagccaca aaacgtgacc agatcaatgt ataaagtttc
    17881 aattgatctt tatctaaata ttgtttgagt tcatctggtg tacgaagcac actcgttgga
    17941 cgcacagctt catgggcatc ttgtgcgcct tgagcatttt tgatctttcg gtgatcatgt
    18001 ctagaaaatt cttttccata cgtatcttca ataaatgctg cggcctctgc ttttgctgta
    18061 tcggcaatcc gggtagaatc agtacgcatg tatgtaatca agccgactgt tccttgttta
    18121 cccaaagtga ttccttcata taattgctgt gctaccatca tggtttttct tgttcgaaag
    18181 tttaacttac gggcagcttc ttgttgcagg ctacttgtag tgaaaggatt cgcagggttt
    18241 cttttgcgct ctttcttttc tactttttta acttcgaagt ctgttccatc aattctagca
    18301 gtgacttctt ttacactttg tgcatctggt aatttctttt tcttaccgtc gattccccag
    18361 aaatttgctt tgaatttctt gcgagctttt ttaaaattac cgtcgatgct ccaatattct
    18421 tctggaacaa attctcggat ctctttttca cggtcaataa taatttttaa agcgacagat
    18481 tggacacgtc ctgcacttaa ccctttcttg accttacgcc agagaatagg actgatcgaa
    18541 taaccaacga ttctgtctaa ggtacgacga gcttgctgtg catctactaa atctacatcg
    18601 atcgttcttg gttccttaaa agctgctttg actgcgtctt ttgtgatttc attgaagacc
    18661 acccgatttt tttctttcag atccaaccca agtaggtacg ctaaatgcca agcaatcgct
    18721 tctccttctc tatccgggtc acttgccaag taaacttttt gagctttttt tgctgcggct
    18781 ttcaggcttt tgatgacatc gccttttccg cgtatagaaa tataatgggg ttcataattg
    18841 ttttcaaaat cgatacccat tttacttttc ggtaaatctc ttatatggcc aacactggcc
    18901 attactttat agtttcgtcc aagatatttc tcaattgtct tggcttttgc aggtgattct
    18961 acaatcacta aatatttata cgccattact gaatggactc ctttccattg tcgtgttcct
    19021 actattaaaa gaggattcat acaaatcgta gctaatcata tccattcaga aaacgtttgt
    19081 caagaaaagc aaatgaaaag tatggtgtta ttctttaaat tctgccaaga tatcttcacc
    19141 cgaaaccgtg caaactgcac cgtcttggat caaatgatga catccggtag aatgttctgt
    19201 taaaatattt cctggaacag aaaaaactgt tcttccaaga tccaatgctt gctgtgctgt
    19261 tatcagagag ccgctttttt tcttggcttc aaagacacat gttcctttag acaaagctgc
    19321 gataatccga tttctcattg gaaaacaatg ctttttcggg ccggattgag gaggatattc
    19381 actcaaaaga agatgatcat ttgcaatcac ctcttgcaag tcacgatttt cagcaggata
    19441 gacttgctct atccctgttc caatgactgc gattgtccgt ccaccatgag agattgctgc
    19501 ttggtgaccg tatgtatcta tccctttcgc gcagccactg acgatcacat atccagcttc
    19561 tatcactggt tttagtaaat aataaactgt ttttttggct aaagcagaag tgtgtcttcc
    19621 accaacaacg cttaacatgt tacaagaaag taaagataga tcaccattat aaaacagtac
    19681 aattggcgga taggtcaagt gaagcagctg agggggatat ttgggatcaa agcaagtgat
    19741 aaacgactgt ttcgattgga ttttgcccca gttagcgtga atttcctgcc aacttttgcg
    19801 aaacagctcc cggtgtttgg ttattttacc tatcttaatg atgtcttcaa atccgatttc
    19861 tttataagga tactgcatca tccagttagc aagtgcccat tttccttgac tactgattcc
    19921 ttttgtataa gcaagtttca ataaattttc ttctatttga tacatcataa aaccgccttt
    19981 cgtctctctt tctaataaga tacgtaaaag cggctgtttt ttattgtaaa tattttttta
    20041 caggtgcaaa acttttacga tggattggcg taattccttg tcgttcaagt ccttctaagt
    20101 gttcctttgt tccataacct gcattatgcg agaaaccata tccaggataa agcacgtcat
    20161 attctttcat caattggtct cgatatactt tagcaatgat acttgcagca gcaatcgaca
    20221 cagatcgtgc atcccctttg attatttttt cctgctgaat aggcaaatcc aaggtcatcg
    20281 catcgatcaa caaatagctt ggtacaatgg acaatttttc tactgcttgg atcatcgcta
    20341 atttacttgc ttcataaata ttcacatcat cgattgtctg ctgatcaacg atcccgatcc
    20401 cgattgccag ggctttttct tgaacttgtt gataaagaag ttctcgcttt ttctctgata
    20461 attgtttgga gtcattcagc cctaatatga tctcattttt cggcaagata actgcagctg
    20521 ccacgacagg tccagctaat ggtcctcttc ctacttcatc aatgccggca atccattggt
    20581 gaccttgtgc gtacccttgc ttttcaaagc aaaacatttc ttcatatttt tccttaatta
    20641 atgcttcctt tttcaatcgt cgttcaaaag aagccagtgc ttgctgcacg cctttgcgtg
    20701 gatcatttct taatgattct agtgtaggat catctattct ctgtatgctt ttcaatcgtt
    20761 ctttgatact ttggatggat tcagtcatca cttttttgct ccatttcttc gcaacgatcc
    20821 agtgtgtatc gtccaagttt tccttgacga atatcatgca cgactaattc gctgccacga
    20881 tcatagtctt ctttataccc acgttttgct gtaatatcca tcagtaattc cggtgccaat
    20941 ttttcttctt gctcgattga taaaccataa cgatcgctga ttcttcctga ataatatcgg
    21001 gcaaaaaagt ctaatccgta aatcgctaaa tcatctaaat ggacaagctg gtctttgatc
    21061 gcgcctgtca gcgctagttt tttgccgatt tcttcatctt caaatttggg ccataaaatc
    21121 cctggtgtgt ctaacaattc caactcatta cctaaacgaa gccactgctg accttttgtt
    21181 acgccaggct tatttcctgt ctgtgcaatt ttcttgccta ctagtcgatt catcaacgta
    21241 gatttcccaa catttggaat acctaagacc attgcccgaa tagcgcgagg cttgatcccc
    21301 cgagcagcat ctcttgcccg tttttctttc aatgcttctt tagccgcagg tactagacct
    21361 ttaattcctt tactttcttg tgccgtgata gctaatgcaa gatggccttg tttacgaaaa
    21421 tattcttgcc acagctttgt ctgttgtgga tctgccagat ccgctttatt caataaaaca
    21481 aggcgaggtt tttgctgaat gatctgatct agcattggat tacgagaaga taaaggtaat
    21541 cgtgcgtcta ccaattcaaa tacgatgtcc acatatttca ttttttcaga aacttctcgt
    21601 cgggcttttg ccatatgtcc cggaaaccat tggatggtca ttgttctctc cattcctttc
    21661 ataccggaat attccccggc tttacttgtt tttttctttt tcaatataaa ttcacttttt
    21721 tattataaag aaattccatc tattagttaa gtgaaagtga accagtcctc attctatcac
    21781 atcttcactt ttttgatgat gcaaattttt attttggttt ctatatatga atctaaattt
    21841 ttgacagcgt ttcctaaata agagatgata gacgaacaaa acagaaagga gacgaaacaa
    21901 atgaatagta tggtttttgt taattttcct gttgctgatg tcaatcgttc cgttgctttt
    21961 tatgaaaagc tgggttttaa aaaaaatgaa gaattttcta ctgaagaagc tggttcaatg
    22021 gtatgggatg acactttttg gataatgctt ctctctcatg atttttataa aaaatttata
    22081 aaagataaag aaatagctga taataaaaaa acaagtggtg ctttgatttc gtttagtgtt
    22141 gaaaatgcgg atgaagtaaa aaaatttgct caaattgcaa aagaaaatgg tggtactttc
    22201 cacgaagtag atatgggaat gcctgaagaa gaaatgtttt cattagaagt ccaagatcta
    22261 gacggaaata cattagaacc cgtatggatg aagatgtaag cagataacta atctgacgtt
    22321 ttttataaca aaaaaacaaa ggcggatgat acgataagta ttctccgcct ctattttttt
    22381 tattcgtaca atctcttttt tacagtttat aaaagcagtc ttctcaccct ttttttaatt
    22441 gttagatgcc agtgtatgta aatcaccaat gtgtcatgac acattggtga ttttatggta
    22501 tccttttatt attaactgac aaaggagaga atattttgaa cactttgatt gcaaaagacg
    22561 tttttagatt gcccgaaatg aatgcaagat tgttgtcgac ctctgctctt ttattagccc
    22621 ttgatgtcat cttatacaag atagcgattg gtcctgctta ttttcaaatc acattcggat
    22681 ttttaagtat ggcgcttatg ggttatctat atggaccgat ctgggctggt ttgcttgaaa
    22741 tgttgtcgaa cactttaaat ttcacgatat ttggttcagg aacgtttcaa gctgcttttc
    22801 ttatcacggc tttccttggt ggatttatta atggcttatt tttatatcat aaaaagataa
    22861 acctgccttc cgtcatcgct gcccaattat tgattatgat cccagtcagc ctttttatca
    22921 attcttggct gatgtctgtt ttgtttggtg cagatttcaa agtaattttt ctaggtagat
    22981 tcatcaggaa tatgatccaa atccctattc aaataatggt tgtttattca tttatcaaaa
    23041 ctttagaggc tagaaaagtg tttaaacgag ctgtttgatt ttacgtacta aggtagtaaa
    23101 ctgattttgg attaaacaat aaagcgtgca gtctcaacag actgcacgct ttattgtttc
    23161 tcactggatc cattttattt catcaagtgg ataatagaca aatcttgctt tacctagaat
    23221 atcagtttta ttgatcgtgc caaacgaacg actatctttt gaaactcttc gattatctcc
    23281 aagtacaaaa taattatctt ccggaagctt ggattgtccc gttagctctt gtagtgtaaa
    23341 gtccgtcgta taggatgcat ttgcatgatc tgatttcagg tttttagtta gataaggttc
    23401 cttaactact ttcccattga tcttcaattg atcattttgg tagctgacat tttctcctgg
    23461 aagaccgatt actcgtttga tataaatcgt gccatctgct aattgaaaaa caacaatatc
    23521 aaatcgtctg atctccgaaa atttttccat cacaaccata tcaccttgct tcaacacgtt
    23581 ttccattgaa ttgccttcca ctggtaccgg gatgaataaa aatccccgca acatgaacgc
    23641 aaggaataca gaaacaagta agtatttgaa tacaagccag aagcgattaa tatagcgttg
    23701 tttttttgtc aatctctatc aatccatccc tctttttttc attctttctt tattataaga
    23761 aaaatatcaa aaaaatgcga ctagatagct gaaataaaaa taattctttc aattaggcta
    23821 ttggacaaaa aacttttttg atcataaagt acaaaactat ctcttatatg tcatgattat
    23881 tttctaaata tttttcccat gcttcgtcaa agatatccat ggacggcaaa tagtcgacat
    23941 tcagttccag ataattagaa atttcttcgt agctagagct gtgcttagga aattggatgt
    24001 ctttaccggc ttctgtagcg aaaacagata aagcatcatg tgtgccaccg cctcgcagtg
    24061 tcattaaata atgaaaaaag cttcgattca cccttcgttc ctccaatttt cttcgataaa
    24121 ggcagcccgg cgtgcatggt ttcttttaaa attttcagga tgctttttat aatactcttg
    24181 atggtcttct tctgctagat aaaatggttg ggctggttcg atttttgtaa cgattggctg
    24241 ttcgaaacgt ccactttcag ctaactgttt cttacttttt tcagcaatga tccgctgttc
    24301 ttctgtcaaa taaaagatca ccgggcgata attgtcaccg cgatcctcga attgtccgaa
    24361 agcgtcagtt ggatcagttt gctgccagta aagttctact aattgttcat atgaaatcaa
    24421 tgttggatca tagataatct ccactgcttc tgtatgtcca gtcgtttggc tttttacttg
    24481 ttcataagtc ggatctggta catgccctcc agtatatcct gacatcactg tatggatacc
    24541 aggaagcgtg tcgaaaggct ggatcataca ccagaagcag cctcctgcaa atattgctcg
    24601 ttccattatg attccccctc taatactact catccgatga ttttttattt tcttttgtct
    24661 tgctttcagt actgctggtg ctagttttgt tggtatttga gttgtttgaa gcatcagagg
    24721 tatcttttgg caaatataga ctaaaccgaa tgtcatcatt tactaagtcg attttatctg
    24781 ctttaatata catcccgttt tgcattcgga attgatccaa cctaagcatg atagtcagat
    24841 catctgtatt gacttcgatc caatctggta atttatagtt tctcttgatc atattcatca
    24901 catctttgat tggcaaactc aatgttccga tagaaagact tctagctttt agttggacat
    24961 ttccgttttc catcacatac ggatcaaaat ataaatagaa atcgatagga aaacctagta
    25021 cttcgaaagt accattgagt agtgcttcat tttccaaata aaatttatat tcaatatcac
    25081 tatctttttg atactcacta aggaaaaagc tgatgatttg attgactttt tctttatttg
    25141 aattgatcgt taagacagga tctccttgtc tttccgtaag agctggtatt tctgggagtt
    25201 ctggttcccg attagcaaaa atacggctgc ctaaaaatac ggtcgaacca agtactaccc
    25261 caagaaggat aaggaaggcg aatttccagt aatttgtttt ctttgttttg tttgaagata
    25321 cgtcagatct tgttttcatg tttattgatc atcctccttc tctaaccatt ggttttgtgt
    25381 ctcgatcagc tgatctctca ctgcatttgc catcaattga taacctaaat tatttggatg
    25441 gaaatgatct tcttcataga gcacattatt tttgatgcca tttagatttt ctgtcgtgct
    25501 agttgtttga tctgttcctt cttctgtgac acctacttcc tgattcaatc cttgataaag
    25561 caaatcattg attgggataa agtagcagtt cggcattttt tttgtcaccg tttctgttcc
    25621 tttgttccaa ttgttaacaa ttgtctgcat gtcagtgatc tcaggaaagt tcaaataaaa
    25681 tggattgtag atgcctaaaa tgtaaatagg tgcggtttta ttgagggatc gaaattcttc
    25741 caatagttct gtgacatttt cctgatagtt tttcagtggc tttttaaagg aattgaccgt
    25801 taacccgaat aaattatttt ggataacttt catcaaatca ttaccgccaa cagtcagtgt
    25861 gataaaatca gctgttttga tattgtttcg aatttcttct tttgttttta ctcgctttaa
    25921 gatctggtca cttcgttcac catttacacc atagttttct gtttcaaccg cggtcaaatt
    25981 ataacggtct tttagatcat ttgcgacaag gggaacgaat cctccttgct tggtttgatc
    26041 tcctactcct tcagtcagcg aatctccgat agctatgtag tgaagttttt cttttttatt
    26101 tggatcttct ctcacagtag ttactggttt cattagtggg ccagctttcg gtatagaagc
    26161 agctaaacct gcatagatga tgatcgcaat taataatggg aacaaaacga ggatcaaatg
    26221 tcgtttcgtt aatttcattt tttcacctct tgtattcgat tggttctaat gttatcaaaa
    26281 attttatcaa aaaggtagtc tttatgaaaa aagaaaacaa ttattttcct aattttataa
    26341 agaaaaagag aataagctgc aagcctttca ggctgagggc ttatcctctt ttcgactaat
    26401 ctacataata catgattgca aacgcgtctt taccagtatg cgtagcgata accggattcg
    26461 tatgcagtac agggatatgc atgtccggga ataattcttg caaaccctct ttgaattctt
    26521 gcgccaattc ttttgcatcc gcatgtgaaa taccgatttg tcgcacattt ggcaattctt
    26581 tcagttcatt tttcaaatta ttaaaccatt tatcgaacgt tttctttcct cgccccttag
    26641 ttacagggat taattcagaa tgggcgaaat tcatcaccac gcgtatgttc aaaacactag
    26701 acagcaaacc agttgttcgg ctgattcttc caccttttac caagttatct agtgtggata
    26761 ttccgatgta taattttgta ttgtcttgta cttttttgac ttcttcaagg attttctcta
    26821 ctgttttacc ttcttttgct aattcagctg ctcgaattac ctgaaaagat aatccttggt
    26881 cagtggtatc actgtcgata accgttactt tcgtttttgt tagattactg gcttgtcttg
    26941 ctgcttctac tgttccactc aagccttttg tcatatggat cgaaatcact tcactgccgt
    27001 cttctcctag ctcatcatac atcgagatga attctccaat tggtggttgg cttgttttgg
    27061 gcagattttt tgctttcgcc atcatttcca taaatctttc gccgtctagt tggtcatcat
    27121 cgggatatac tgttccgtcg atcatgatgg atagcggcat gacacgaatg cctaaagcat
    27181 cgcgtacatt tttttccatc gtacatgaag aatctgttac gatttttaca tttgtcatta
    27241 ttttctctct ctctttcttt aacaagatgc cttattcatg ttaaactaca tgtaagacaa
    27301 tttctatttt tagtataaca gacaaataat gatttttcat taattacacc tagtaaagga
    27361 gccgttttca tggaaaaaac gaaatttaat cgaacgtatg aaatattaaa tgaagtgttt
    27421 aatgcagtca cccatggaat tgggactgga ttgagtatcg cgggactagt ggttctttta
    27481 gtaaaaggtg cccacttgca ttcctccctc catattgtct cttatgctat ctatggatcg
    27541 atgatgattc ttttattttt aacgtctact ttgtttcatt cattgatttt tacaaaagca
    27601 aaaaaggtgt tccaagtatt cgatcatagt tcgatttttc ttttgattgc aggaagttat
    27661 actccttttt gtttgctgag tatccgagga tggctaggtt ggactttatt tgtattaatc
    27721 tggttgtttg cgattcttgg cattatatat aaagcgttga cgttgcataa acgggagaga
    27781 gtatcaaaag tatctacgat catctatatt tttatggggt ggctctgtgt cgtggctgct
    27841 gttcctcttt atcactctct tggaatgact ggtgttattt tgatggtagc aggcgggctt
    27901 tcttacacga taggcgcatt cttttatagc ttgaattcta tccgctatat gcatgttgtt
    27961 tggcatttgt ttgttatgtt aggtgcaggt tttatgtact tctctgtttt attctttact
    28021 taataaaaaa aataaagcta tggatgggac agctctaaat aacagctagt ctatccatag
    28081 cttttttata tcgtcaattc gcctgtcccg ctttttacag gattctcatt acgctgatac
    28141 gtttcaaatt gatgcggata acgatttttt tcatccattt ctccttgact agtattgatc
    28201 agtgaccaat cagaccagtc aacaggtgga aaataagcat ctccatcgaa tacatgatgg
    28261 atgactgtac ggtataatat cgtacaaaat ggcagaaatt cttgataaac agctgaccct
    28321 ccagcgatga aaaagatacc gtctgtttct ttttccagag caagcacgtc ttccacacta
    28381 tgcatgacat gcacgccttc tgctttatag gaagaatctc tcgtcaaaac aatcgtctga
    28441 cgattcggca acggacggct acccatccct tcgaatgttt ttcgtcccat cactagtgta
    28501 tttgcttctg tcatttgttt gaagaatttc agatcatttg gcaaacgcca aggaagacgg
    28561 tcttcttttc caatcaatcc gttttcatcc tgtgcccaaa ttgcagctaa cattttctca
    28621 tcctttctga ttctaaacag cgataggagc tttgatcgcc ggatgtgggt catatccttc
    28681 taattggata tcttccatct caaaatcaaa tactgactgc ttttcctgat tcaaaactaa
    28741 agtcggaaat gcgcgtattt ctcggctcaa ttgttctttc atttgttcga tatgattgat
    28801 ataaagatga gcatctccta atgtgtgaac aaattcgcca accgctaaac ctgtttcatg
    28861 tgcaattagg tgagtcagca acgcataact tgcaatgttg aacggaacgc ctagaaacac
    28921 atccccactg cgttgataca attggcagct tagccgtccc tcttgaacat aaaattgaaa
    28981 catcgtatga caaggaggca aagccatgct aggaacatct tccggattcc atgcagaaac
    29041 gatcaatctt ctagaatcag gagtcttttt gattgcttcg atcacttgtt tgatttgatc
    29101 gataaagcca ccgtcttttg tttcccagtg acgccattga tagccgtaga tgtgtccaag
    29161 atcgccgtat gtttcagcaa aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct
    29221 ctgcttttta tattcttcat tgaattcagg atctgtcaaa catctatggc caaaatcagt
    29281 catatctggt ccattatagt ctggactttt gatgtagcgt tcaaatgccc attcatccca
    29341 aatatggttt ttattttgta atagatagcg gatattcgta tctcctttta aaaaccaaag
    29401 caattcgctt ttgatcaatc caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt
    29461 ttgcagatca aaccgcattt ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc
    29521 ttctttacga tgcccttctt ccaacaattt tcttcctaag tcaagatacg cttgttccat
    29581 cctttttcct cctaatcttc tacccattca ctcaagtatt cccaccgtgc cattttttct
    29641 tcaagttgtg cttctgtttc agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta
    29701 tctccttgat gattcatctc ttcttggagc aatgagattt tttcttcaag ttctgcaatc
    29761 tcatcttcaa tcgtttccca ttctttttgt tccatgtaag ataatttctt cttttcagca
    29821 ggttccttgc tctgtatttt tggtttcgct ttttcagcag gctgctgggt ctctttttgc
    29881 ttcagtaaat aactggacat gtttccgtaa taaagttctt gttttccgtt tccttggaat
    29941 actaataact tatctgctac cttgtccaag aaataacggt catgggaaac agtgatcaca
    30001 gcacctggaa aacttctaaa ataatcttcc aaaatcgtca atgttgctat gtccaaatca
    30061 ttggtcggtt catccaataa taaaacatta ggttgctgaa tcagcagttt caacaaaaat
    30121 aaccggcgtt tttctccgcc tgaaagtttg cctatcagtg tcccatgcat aaatcttgga
    30181 aataaaaatc gttcaagtaa ttctgctact ccgatttgac tgccatctgc tgtttttgct
    30241 tcttcagcag cttcttgtaa gtaagcaatc atccgtttat ctggtgccat ttcttcattt
    30301 tcttgtgtat aatacgcaag atgaaccgtt tcaccgatag acattgtacc gctatccagg
    30361 ggaattctgc cagctagaat attaagcaat gtcgattttc ccgcaccatt tttaccagta
    30421 atacctagtc tttcccgaga ttgaatcaat aaatctaaat gttctagcag tgtttggtca
    30481 tttatacggt aactgccatc tttgatttcc agtacttttt tccctaaacg ttgtgtcgct
    30541 aggttcagct ctaaatcatc ttcttggttt acttggtaca agttctcctt tagatcttcg
    30601 aaacggttga tccgtgcttg ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa
    30661 gctaattcct gtttataaag ccgcttgcgc ttttcttctt gttctacttc tacacgatca
    30721 cgttctgctt tttctaagac gtaagcttca taatttcctt tatactcgta taattttcca
    30781 aatgaaagtt cgaagattcg gtttgctact ctatctagaa aataacgatc gtgggtaacc
    30841 atcaatagtg cgccacggta ttgtttcaaa tagttttcca accactcgat cgcttcataa
    30901 tctaaatggt ttgttggttc gtctagtaat agtaaatcgg gttcatcaat caacacttgc
    30961 gccagactaa ctcgtttttt ctgaccacct gataaagtac tgattttttt atctaattca
    31021 ctgattccta atttttgcaa aataatcttt gcgttggtat ctgtagtcca agcatcttct
    31081 ttattcattt gttcttctgc tttagcataa cgtttttgca catcagggtc ttctccgttt
    31141 tcccctagtt cgatcaatgc ttcctcatat gctcgaacag tttggatcaa ggggctgttt
    31201 ccttgaaaga ctgcttggag aacagttaat tcatcggaaa attcagctgc ttgagaaaga
    31261 taaccaatac ggtaatcagt tggataaaac actgtttgcc gatcaccgtc gctgctatcg
    31321 ataccggcaa taatatttaa gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg
    31381 atacggtctt tttcgtggac caaaaaagaa atctggtcaa acaaatcttt ttcaccataa
    31441 gtttttttga ggtcagttac ttttaattct ttcaacatgt tccctcctta tcttggatat
    31501 tgtatcataa aaatagtgaa cttcaggaaa atttttttgg tatttgttct ttttgtgttc
    31561 tcaaaaaaag aacaggaccg attcgatcct gttctgatat gttttgtttt ttataaaact
    31621 tgaaaatttt taccttttta ttttattgga ctcgtttttt ttgactttgc tcgaaacgca
    31681 aatcggaaag aaaagttatc agatggtttg ttcatcgtat aagattctaa caaattgata
    31741 ttcctgcgat agctgttcgc agaaaacgtc gtgatttttt cagcaacttc gtattcatca
    31801 atgatttttc tttcaagata ataacctctt agcatttttt tcgtaaacag actatcttgc
    31861 tggatcatcc acgaagaaat caaattcccc gagttcattt ttattccttc aagtttgcgt
    31921 tgctggataa aaaattgaac catcgtttca tcataaatat cagtcaagtt atctaaactt
    31981 tcaataatca actgagtatt tttttgatat gtctctctga tttcttggat atcttcatca
    32041 ctaataaatg tctgacgatc agcatatctt ctttgccaaa acattttcgg atggagaacg
    32101 attcgcatta acctacggac aacaatgaac caaaagccga taaaagacaa aatctggctt
    32161 gtgatagaat gttcgaatcg agaaagaaaa cgggagtaaa aacgataacc attagaagaa
    32221 agcttgcccg aacgataact ttcatccaat ccatcccgtt cgatcgacaa aatcagtgct
    32281 tgtatttctt gtacttcttg cttttcatca tcggttaaat cttccaagta cagatcacgt
    32341 atccgttctt gataattttc tatcactgct tcagtggcta aaaattcttt tgtattacga
    32401 tcaatttcct ttatttcttt tcgcaaaact tcaacgacct cttctaaaat catcaaagca
    32461 ttcaaatccg tgcttttcgc ttcctctgtt tccgtcagca taggcaagac aatgatccct
    32521 atgaccaaag tcactaagat gacacccgcc gtcagaaaca aaagcaaaga ccgctggtaa
    32581 aacatcatat tattgattga aaatggcaaa ataaaaatcg tagccaaact gactgttccc
    32641 tttacaccgc caaatgttaa gatcaagatc tcgttcaact gttttttgaa ctttttactg
    32701 ccatctttga atacataaaa aagactgata gaaatgaatc tgatcacaaa aagcatgaca
    32761 gagattaata cgataatcgc taataaaaga ccgtttggat ataaaccgtc tccccatact
    32821 ggcgagaaca cttgcgtcaa ttcgattcct aaaaatataa atactaatgc attcagtgta
    32881 aaagcaatag ttgtccatgt actgtgggac aagctcgaca attctgcatc aaacacagag
    32941 attttcctaa aaccagaagc ttggagaata cctgctgcca ctgcagcaat gattccagat
    33001 acatcaaatg cctctgccaa tacataggct agaaaaggaa gcaaaagttc gatcagcaga
    33061 taccctgtga catcttgtgc tgatgctttt tcgatcaaat gaattatttt ttgtttgaac
    33121 cagacaagca aaaagccgat caaagctccc ccaatactag aaactactag actcatcccc
    33181 gcgtttactg ctgaaaaaga tccggtgatc agcgcaccaa gagcaaactg gaaggcagta
    33241 acaccagatg catcattcaa aagaccttct ccttccaaaa tatgcatcgc ctttttaggg
    33301 atattcactc gtccagaaag agaactgact gctaccgcat ctgtcggacc taacgctgct
    33361 ccaaaagcaa agcaggcagc aagtgggaca cttggcaaca ggaatgataa cgtcgcacct
    33421 accccgacaa gggtcaaaat gactcctcca aatgccaaga acaaaattgt cccgaaattt
    33481 ttgagaatcg aagaaatatc tgctttttct ccttctctaa ataacaatgg tgcaataatc
    33541 ataactaaga acaattcagg ttcaaaatcg attgattgtc cccactctgt cagacctaat
    33601 accactccaa ggaaaatttg gatcagtgga gctggaatca ttggcaaaat gcggctagct
    33661 atgttcgata aggtgatcgt tactgcaaat acaataacta actcagcaaa ttccatcgtc
    33721 catccccttt attttttatt ttgtgtaagc atttttaaaa attgttcttt tttctttaaa
    33781 attttggttt cgactacttc aacctgtgtt tggttcccct ctaattcaca ctgtacacgt
    33841 tttgactcca aacgaagcag atcatgtttc agacgttcca actgacgata catcaatata
    33901 tttcttttac gcaacttaca aagtatttga cggcggtcat ccatcgaaag ggcgccatag
    33961 cgatcatgca acagtttttt ttgttgttca aaactctctc tcattttcca gatctcctat
    34021 acacaaactt ccatcattat agcaaagttc tttttggatg aaaaatattt cattgtttca
    34081 tcgtttattg ctagttagaa atttccataa atttttcaac gcctcattct attccaaata
    34141 gtatcagata tcaagtaaaa cttaagaaag ataaaagttt ctgacaaaag gatgtttact
    34201 ttcccattcc tgcctttctt gttcttttat cacaacaaaa aaatggggct gcgacaaaaa
    34261 tcttgtcaca gtcccttatt ctgaataaac ggtgtggaag agacagccta tcagtagtaa
    34321 taagacacat ctctttcaca gcctcatttt tgcgtaaaaa tcgatttatt cgaaaaattc
    34381 cccgtataca tcttctacac gttgatctgg attgatcacg atatacaagt tacctgtttt
    34441 ttcgctgatt tttgttgatt gatattgatt gtctaatcct tctgttcctt tatctagata
    34501 tgggaagtac acaagatcag gtgtgccatt tcggtaaagg atttccattc gctcgatatc
    34561 ttcgtatttc aatgcgcgat taaacatgcc catttccagt ccgcctaggt tgatatcatg
    34621 tgtcgcaact gcgtcccctt catggaagat ttcgatttta aatccagcac acggatggat
    34681 ttcaacaaat tcactgccat tgattcgtcc aaaacttgtt gtgatttgtt tgatccaaag
    34741 atcgccgata tgacggcggt tgatcgtcca ggtttctcca tttctaaaat aaaattttag
    34801 cgctgtcatt tctttactca ttaaaaagac tcctttatga tgtaatttat tcttgtacta
    34861 cctcagttta gcacaaattt atgtaagtgg tcacatctta tgttttttct tctaatgacg
    34921 tatctgccca ctttaagtag ccaacaaagc tgagcagata cagtctagtg ttttttaaat
    34981 caatttttct ttcgataaaa aatccaaaac ctgtttttta tcatttttta ttctttgttc
    35041 tacgacggct ttttcagcta attgcaaagc ttcactaacc cacttaccag gtttttttcc
    35101 agtttcttct agcaagattt taccatctac tgctaattcg cttaatgact tgataggtaa
    35161 acgatcatag gattctccta cggcatccat ctgaggtgtt tcgtcaaaat agatcatcaa
    35221 ttcttctact tgcagtgcat gttttttgcc taagaagtac aattcgaatg gttccaaagc
    35281 tccagtttct cgttgtcgta gtccttggac aagtgcttga acgtctttaa tcatttggtt
    35341 agaacacttc cacgatttca aaaaggaacg aatctcattt tcttttagat ccagttggct
    35401 gatcaataat gcccatgctt ggctctcttc tttcaatggg acggatggta attcagcaaa
    35461 acgtaaaagt gcttcgcctt cttctttcaa accgggacag caaatgtagc actctgtttc
    35521 aataaatgca tggattcccg cggcccgttc atttcccaag agcatcttga tgaattccac
    35581 atttatacgt tctacagata ttttacctaa tagctcgtgg ttttctttga tggcgtagaa
    35641 ggtttcttcc tctaatttga accctagttg actgacaaaa cgcaacccgc gcatcattcg
    35701 taaggcatct tcatggaacc gctcatgagg attgcctaca gctcgcaaga tctgttcttc
    35761 tagatcttgc agaccatcga acaaatcgat gatttctcca tcttctttca aagcaaacgc
    35821 attgatcgta aaatctcttc ttttcaaatc ttctgccaac gaacgaacga attcgacatg
    35881 atctggtcta cggtagtcct gataagttga ttccgtccga aatgtggtga tctcatattg
    35941 ttcatcattt tctaatacca acacagttcc atgttcgatc ccaatatcta tcgtttttgg
    36001 aaaaagctgt ttgatttctt ctggaaatgc gcttgttgcg atatccacat catggattgg
    36061 ctggttcagc aagatatcac gaacacttcc acctacaaaa taagcttcat atcctgcatt
    36121 ctctaatttt ttcagaactg gaattgcctt ttggtattcc agaggtaatt gttttaattt
    36181 catttttcca acctttcctg cgtgtttctt gtcctgagat aaagactcag ttgcatggtg
    36241 actgtttgac aagtccgttt cagagtcttc aaggccagga ccacttcatc ttgtttactc
    36301 gtccttacgc tcaaagcgat tatggtctct ttgattgaat ttttccatgt ttttttcaaa
    36361 aacttcagtt aagtcgatat tcaatgaatt agccatgatc atcgtaacga ataaaacatc
    36421 accaagttct tctgctaccg agttaactgg ttcagaagcc tttttgcttt tttctccata
    36481 agtgtggttg acttctcttg ccaattcacc tacttcttca gttaatcgag ccatctgagc
    36541 taatggagag aaatacccca ccttgaattg ctggatatat tcatccactt cctgctgcat
    36601 actgtataaa gaacgctttt tatccatctt tttccctcca aaactatctt accaaaactt
    36661 taacgcaaat cctagataag agcaattgag aaaatcgaat ttccatgcta catttaggaa
    36721 ggaatttcta aatggaaatt ctgtgaaaat acataattga aagctggaat tcatatggtc
    36781 aaacgaaaaa cagtaaaaga agttcttttt atcattatag gaacaagtat ttatgcattt
    36841 ggtcttgttt acttgaacat cgctaatcat ttagcagaag ggggagtctc agggatcacc
    36901 ttgatcttaa gagctttatt tggtattgat ccagcttata cgactttatt aatcaacatc
    36961 ccattgattt tactaggagg aaagatcctc ggtaaacgtt ccttggccta tactgttcta
    37021 ggtacacttt ctttgtcatt ctttttatgg atctggcaaa gggttccact agaaatcaat
    37081 ttacagcatg atctactgat cgtttctcta cttgcgggtt tagttgcagg tgtaggaagc
    37141 gggatcgtct accgcatggg tggaacaaca ggcggcagtg atattatcgc tcggatttta
    37201 gaacaaaatt acggcatcac tatggggcgt tccctattgg catttgatat catcgtctta
    37261 cttgcttcat taacctatat cgacttgaaa cgtatgatgt atacgttgat tgtttcgtat
    37321 gttttcagtc gtgtgattga ttcgatctta gatggcggat attctgcaaa aggtctactt
    37381 gtcgtctcca acaaaagcga agagattgca ccactactga tgaccggtct gcaaagaggt
    37441 gtgactttcc tttctggaga aggcgcattc tctggtaatt caaaaaaaat catctatatg
    37501 gtcgttagct ctagtgaatt aactgaagct aagaggatcg tccacgagat tgacgaaaaa
    37561 gctttccttt caatcatcaa tgtgcatgaa gtggaagggc aaggctttac ttatttgaag
    37621 ccacagacaa gattactgaa aaaactataa gaaaaaagac aaagataggc tatgaggaaa
    37681 gattccacat ccagctgacc tttgtctttt ttagtactat actattttta actaattgtt
    37741 atgttcactt acccggttac ttttcttttg tgtctgcaaa aggatctttc ggttaacttc
    37801 atcagataat ttcaaaaaag catccctatc tccatcttct agtgcttgat cgatttgagc
    37861 ataaagcatt tttagttttg cctcttcttc tttttcttga aaatatcgtt caattaattc
    37921 agtatcttct tcactgactg aagaatccca agaagaaaat ggattgtctt ctaagataga
    37981 tagatattct cttgtctgcc aagcattttc gaatatacat tccacatata aaggcttttt
    38041 ccaattcaaa cggatttcat gaaaaatctg gtctgtgtcc gtaaattctt ttccctgcaa
    38101 aaacaatttc atgggttccc cttcaacaga tagatcactg atctgcagtc ctcgatccgc
    38161 aaaattcgcc ccttcaacaa agtggacatt gcttaaaatc gcttcatgat tggcaagata
    38221 attcaagatc caaaaaactt ctcttcggct aaaagataca tgattgacca tccacgttaa
    38281 aaaattcttc ttttcccgta catcaattag catttttctc acctctctgt taggtcatct
    38341 aataatgaca gtgcctccat atctcctggt tcaagtgtta aataataagt caataattct
    38401 tgcgctcttt tcagctgccc ttcttctctt aagaaaaagg catactcttt caagaaatct
    38461 ggttcatgtg atagttcatg ataagcctgt tcatagtgta cagctgcaac ggtaaagtct
    38521 tctaattcat tataagcatg ggctaggttc cattctgcat aaggattagc agtttcttcc
    38581 atctgattga tcacttgtat cacatcttca tatctttcat catccaagta aagattactt
    38641 aaagtaagta atgtttcatc ttgtttgtct cctacttcta aagctttcag taaaagttct
    38701 tctgctcgct gtctatcatg cagacggaaa gcattttccg aggccaggtg ataaagttcg
    38761 acttggtaag gattttcttt gattccttct tctaacactg tttgtgcttc ttcaatcatt
    38821 tcttcttctt gaagagcttg tcctagatat agatataacg attgataatg cggattaact
    38881 tctcgaagtt cttccagata gttgattgcc tgctgatttt ctttcaattg aagattcgta
    38941 aaagctagct ggaacagtcg atcatctgtc ctttcttctt tcagtgcttc ttctaaaggt
    39001 acgatagctg cttcgaattc tccttgcata cttaaggatt gtcctaaacg ttcctgcatc
    39061 gagattcctg aaatttcatt tatcccgctt gccaacaatg tttcataaac cactgctgcc
    39121 tctttgaaac gatcaactga aaaatacaat tcagctagcg caaactggat caatggttca
    39181 tcaggaagca acccagctgc ttctttcaat tttgcttcac tcacttcagg gattccgata
    39241 acttggtaca aatcagctat cgccaataag ctttgcggat agtaatcact ttgcttatcc
    39301 actttttcca ggtattcaaa agctaagtcg atctcattat cttcgattgc aatttctgct
    39361 aacgggatgt tcagtccgtc attttctggg tattggttga tcagttgttc aaaaatctgt
    39421 tttgcttcga tcaaaaagcc aatagccagc aattcttcgc ctagttcagc taatatatct
    39481 gctgggtctt cttttaatgc tttttctaaa aagatttgtg cttgtgacaa gtcttcatct
    39541 gataaagctt gcaacatttt ttcgctattt gtattcattt caattccctc ttatctcact
    39601 gacgatttgt tgaaataccg tcattgttct caataattct ttttcgtttg gatgttggta
    39661 tccgtctatt atacctattt tttctaaaac aagtattttt ttgctttttt ctgccaaaaa
    39721 aagaactttt tccaaatact ccatctttga aatctgttcc agccagtgga ttggatagcc
    39781 aagcaagtcc agccacacaa aaaagttttt tagttggaaa tcaaaaggat atgcttggag
    39841 attccacacg aaatgaaaca tcattcctaa aaattttttc atactagcag aaagtagatg
    39901 gccattttcg gtcagataaa aagcttgttc aaacagttta ccataatttg acaggttttc
    39961 tgcctctaat tgataaaaat agatcatttc ttcgatcatc ccggcaaacg gccgagtcgt
    40021 taattgcttt ttatttggat agtttttata cagattttgc aaaaaacgat gatcacaaat
    40081 cattccgcaa cagataaaag tcaaaaagtc aatctgcttt ccgtccacct gccgctcgct
    40141 gattgtttga tcataaacga tgcgttctgg cagattgatg gtttgcagca ctacacgatt
    40201 tggtagctgc aaaatcgtcc gctgcggtgt taatgctcgg cttagagaac ggatcgatac
    40261 agggatcatc catagtgatg aatcaagggt tgtatgtttt tgaaaaaaac ctgccagatc
    40321 catgatccct tcatttccaa atccgatgat aaacatggat tgattttcgg gatagcgttt
    40381 gacgaaatca agcaggttct ttaattctgt taaatgattg cattgagtgt tggcacaaat
    40441 ataccagtca ttgtttgctt gatttgcaaa taacgggttg atcttatctg caaacaaatc
    40501 ataatagcgc tgattcgtta aaaataagat tggctgatca atggaaagtt ctcttagctg
    40561 actgactagc gtttctccgt aaaacacttt cgtcaacgca tcttctactt gataatcaag
    40621 ttccatacgc atccctcctc ttcttatttt tattttacca ttagtaagca aattctagaa
    40681 aagataagag caaagctcac ctcataactt gcaaataaat aaaaattaaa taaaatacat
    40741 gtgttttcta tgtatgacat acggattcgg aatctctgtc tcttatttat tccttttctg
    40801 ctaggaaata aaaagaaaag cacttgtagc cctagagcca caagcgcttt ttcaactgtc
    40861 ttatttaaca gcgtctttta atgctttacc tggtttgaat gcaggtactt tgctagctgg
    40921 aatttcgatt tcttcaccag tttgtgggtt acgacctttg cgagctgcgc gttcgcgaac
    40981 ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg ttagctaaag catcttgaat
    41041 agttgaaaat acagcatcta ctgctgcagt tgcatctttt ttagttaaat cagtagctga
    41101 agcaactttt tcgattaatt ctgctttgtt tgccatggat agattcacct cctccacaag
    41161 agatatgata gaaatatcat ttagttaaaa ctttctgagt ggtccagaag tttaaaaaga
    41221 aatattgatt aacttcaata ttctgttatc acgatatcat atgaaccgct taatagcaag
    41281 gtttttacca attttcacat gcttttttta gtcatagaaa cgtaacaaac gaaactgtct
    41341 ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct attttctcct acgagggata
    41401 attcggatgg acgtcccttc aaaagtaaat gcttttctga tttgattttc taagaaacgt
    41461 tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa cgacaaatgt aggtggtttg
    41521 acagcgactt gtgtagcata aaaaatcttc aggcgttttc ctttatctgt tggtgttgga
    41581 ttgattgcta cagcatccat aatgatatca ttcaacacag ccgaagagat acgaagattc
    41641 tggttcatac ttacttgttc gatcatttca ggcagacgtt ccaaccgttg tttagtcact
    41701 gcagaaacaa aaacgattgg agcgtaatca agataccgga attcatcacg aatctcttgt
    41761 tcgaaatcac gcattgtatt ggtttctttc tttacaagat cccatttatt cacgacgata
    41821 ataattccac gaccagcttc atgggcgtaa cccgctactc gcttgtcttg ttcccgaatc
    41881 ccttcttcag cattcagtac catcaaaacg atatcagaac ggtcaatggc acgcattgca
    41941 cgcatcacgc tgtatttttc agtattttcg tatacttttc cgcgtttacg cataccagct
    42001 gtatctatca ttaagaattt ctgaccactt tcagaaacga agtgggtgtc gattgcgtct
    42061 cgggtcgttc cttcaatatc cgaaacaatt acacgttctt ctcctaaaat cgcattgatc
    42121 aaagaggatt taccaacatt tgggcgacca atcaaactaa acttaatcgt gtcgtcgtcc
    42181 tcttcttcag tctcttcaga gaaatacttc actgcttcat ctaaaatatc accaatccct
    42241 agtccatggc ttcctgaaac aggataagga tcgcctagcc ctaaggaata aaattcatag
    42301 atatcgtttc tcatttctgg attatctact ttattgactg ctaaaatcac tggtttatta
    42361 cttctgtata aaattttcgc aaccatctca tctgcgtctg tcacgccttc gcgtccgctg
    42421 actacacaaa tgattacatc tgcttcttca atcgcgattt ctgcttgatg cttgatctga
    42481 tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt cgatcacgct gaattctcga
    42541 cctaaccatt caccttttgc ataaatacga tcacgagtta ctccaggagt atcttcgaca
    42601 atcgagatac gctcaccagc gatgcggtta aaaatcgtag acttaccgac gttcgggcgg
    42661 ccaacgattg caattgttgg atttgccata ttatttcctc ctcacaagtt tccaatctca
    42721 cttagtttaa cgaatgcatt gccaactttc aagcatagat tgtcaattaa acgaagtatt
    42781 tttattcctt attgaaatac taatcgcttt tcaaaagaaa aactgagaca aaagcagctg
    42841 ctttcatctc agtcacagat acaaaatcaa ttatttttca gaatcatcgg ttgctttttc
    42901 ttctacttct tctttcaacg catcacctaa aatgtctccc atagtaaatc cagtattttc
    42961 ttctggtaat tcataagact caacattttt tggttcttct tctgaagctg gtttttcttc
    43021 taaagctttg atactcaatg caatacgatg ttcttctgga tggatctcta atactttcac
    43081 tttcacttcg tcaccttcat gaagcacttc atgtggtgtc gcaatatgtt tatgtgaaat
    43141 ttgagagata tggactagcc cttctactcc agggaacact tcaacaaagg caccgaagct
    43201 tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact ggagcttttg tatcgatttc
    43261 tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa acacgttctt gttcaggatt
    43321 gatagacaac actttgacat ttacatcatc attgacagcc aatacatcac ttggttttgc
    43381 tacatgacta tgtgaaatct ctgagacatg gactagaccg tcaattccgc ctaggtcgac
    43441 aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg agaacatctc cctcatggat
    43501 agttgaaaga atttcttttt tcttttcttc tctttcgttt tccactaccg ctttatgcga
    43561 taagatcaaa cgattttctg atggttcgat ttcgatgatt ttaaagctca atgttttgcc
    43621 tttatagtct gaaaagtctg caacaaaatg atcttccacc attgatgcag ggacaaaacc
    43681 acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca tttgtaacag gtgcttcgat
    43741 gaccttgccc tcttggaaat ctctttcgat atcttcccaa acttttttag catctaaacg
    43801 gcgttttgaa agtagatagc tgccgttttc tttatctttg ccgatcgtac tgataacgac
    43861 taaatctaat acatcaccga cttttactac ttcgctgata tcttctaccg gcaaagttga
    43921 taattctttt gccggaacga ctccttcaac tccagcccct tcgattccga caatcacttg
    43981 tttgtcttca atgactaaaa cttcgccctt aacaacatcg ccaactttta cttcttgtac
    44041 gcttttcatt gcgtcttcca ttgactggtt gttctcttga ttttgatcaa attctgtcat
    44101 aacacttgtc ctcctaacca acattctgcc gtaagtacgg tactgttttt agtttaattg
    44161 attattcaaa aaaaataaag cgatttcgtt cgttttcctt gaaaaaacaa ttgtttttca
    44221 ttttttttct taatttaaaa aagaagataa ccttttttca aaacaacagc ttttattgct
    44281 gcaacgactt ctggaatcga tttgccagtc gtatcgatac gaacagcatc ctctgcttgc
    44341 ttcagcggag agacgtccct tgttgaatca taatggtctc tttcctgtat ctctttcgtc
    44401 agtgtttcaa gatcagtcgg aattcctttt tcttggttct ctttgaaacg tctttctgca
    44461 cgctcactta cgcttgctac taaaaagatt ttcacttctg cattcggtaa tacagctgtc
    44521 ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc ctatttttcg ttggacagca
    44581 actaattctt ctctgacttt tccatgtgca gagacttcag aaacagcttt tgtgacatct
    44641 ggttgacgga tttctaaagt cacatctttt gatgctatga atacatgctg tccatcttct
    44701 tgttgcgaaa aagagatagg atactttttg atcagcgaaa ctagcccttc ttcatctgaa
    44761 aaagatacat tgttcgtaat agctaaataa gttactgcgc gatacatggc accggtatct
    44821 gtataaatat aattcaattc ttttgctaat atcttggcaa cggtactttt cccagatgaa
    44881 gcaggcccgt cgattgcaat actgatttgt gtcatgatat aactcctttt tctcagaaaa
    44941 aaagccgcaa agcgcggcta ttattattta attcttaatt cttgaccagg gtaaagcata
    45001 aagttgtttg gatctaagcc attcaattca aacaattgat cagtggtgat ccctgcacgc
    45061 tcagccactt gttttggacc ttcgcctgct tgaacagtcg ttgttgaacc ggctgcagct
    45121 gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat cccctgcttg ataagttgcc
    45181 tcacttgagg aagtcggtgt tgtctctgct gcaatactgc tttctgacgt tacacctgta
    45241 tcttgagaca cagtagattc ttccgcgatt gtactagatt ctactgttga agtctcagtt
    45301 gactcctctt ttgttgtact tgtagaactt tctactactg atgtcgaagt ttcagaagaa
    45361 gttgctgctg ttgcattgtt gcttgatcca tttctgatcc agaaataagc acctgctgga
    45421 atagcgatac ataagaccag taaaatcaaa aatagcgaaa gaaataatgt atttcctttc
    45481 ttttgttgtc tctgagaact tcttgatgaa acttcatcat ctgtatcgta gattggttgt
    45541 tcccatggtt cttgtgtttc gttattttct gtagattgat gtctatcttt tctgctcact
    45601 gttttacctc ctaaactcta tcgtgtgtat tataccatag gaaaaagtgt cttgtcctaa
    45661 aaaatcaatg ttccatgttt tttttaaata aatttagtaa aatcgttccc caatgaagtt
    45721 gttcagtctt agaaaaaagc ttcctctttg gcagaagttc tgcttttgct ccatcaatat
    45781 cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa ataagctaag atgaattttc
    45841 tgcggcagtt ttcttcatta atatagcgaa gcatctgctg caggcggaat tctttttctt
    45901 tttcctgacg tttgagccgt tccacccaat tttctggttt ttcagactgc tggatcagct
    45961 ccatccattt tttttgcagc tcatcaaagg gagcttgctc tgtggcatat tccaaataaa
    46021 gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa gaaataatgg atacgttcat
    46081 ccccactttg ataaaaaaga attgctgcac tttcttcctg atctctgcct gctcgaccga
    46141 tttcttgtac atagttttct agactgtctg gcaaatcata atggaccaca taacggatat
    46201 cagatttatc gatccccata ccaaatgcat tcgtagcaat gagaaactgc agctgatttt
    46261 ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc aagaccaccg tgatagtatc
    46321 caactgtaaa acgttccctg aataaatgat acaatcgttc gacttctttt tttgttgcac
    46381 aataaatgat tgccgctcct tgtgctcttt ccatgaagtg ttcaagttct tgttcttttt
    46441 gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt gactgattgt ctgatctctt
    46501 ttggtggtcg atcaaatagt acatgacgta tatctcttgc tactaaatct gttgctgtcg
    46561 ccgttagtgc cagtgtgacc gggtttccta aaagctttcg gatttttcct aactgttgat
    46621 attctggacg gaaatcaact ccccattgag acacgcaatg agcctcatct actacatata
    46681 acgcaatatt ttgttttttc agatgctcta aaagagaagg attagtcaac atttcgggac
    46741 tcaaaaacaa aaatttatat tgcgatagat gacggaggac gtatcttctt tcagattgcg
    46801 aaagtaaact attgaaagca gctactcgtt tctcccctcg tttttgtaat tgcgtcactt
    46861 gatcttccat tagagaaagc agcggtgaca cgatgatgac cagaccttcc attaaatacc
    46921 ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg caaaatagcg agagtatctt
    46981 ctccatcaag caacgcctga ataatttctt cttggccggg acgaaatgaa tcaaatccaa
    47041 aatatttttt taattcattt attaacattc tgtatcgctt cctttctcaa tagatagatt
    47101 tgatacaaac gaaattctcc ataatctaat tgtccatcta cattccattc gctaaagcgc
    47161 cactctcgca cggaatcctt atttggttcc aaacgttcga ttgtttctgc agagagaatc
    47221 cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa taagatgatc tgttactgtt
    47281 cctcttttta aatgacggat tgccatgatc tggtcaattg tctgaccatt cataaacatc
    47341 tgcttggtga tcatcatgct ttgattgaaa ttttgctgta ataatgaatc gatcaaagcg
    47401 tgccaaagcg aatctggccg tttttcgatt tgtgctaaca aaaggtggat acatctgctt
    47461 tgttgcagat aaaccgctga ctcatcattg ttttttgcca gctgataagg aagcagccct
    47521 gttcgctcat ttccagataa ttggttagct aaaaaatctg ctgcttcttc tggtatctcc
    47581 gaaaacagct gcaccaaact ctcatacgca gtatcaatca aaatttcacg tggcaattgg
    47641 ctttcactaa gccatttttt tagttggaaa gtataaaaag gacttgtctc tgctggaaga
    47701 tagtcttgaa tacccgttgc cagattagaa attacttgaa cagcaaattt aatgagccgc
    47761 caactagttt cccctgttct gccatagcgg tcaaaacgca aaccagtatg ttctatttga
    47821 tgttcggaca acatatcagc ccctgcaggc gttagctttg cttcattttc atttatttcg
    47881 atccaacctt gctgcgcaat tttttgcaaa atttgataaa acttatcctg cttcaattct
    47941 ggaaaactgt tatggataaa caaaagttcg tgggtaaatc cataaataag tactgaagtc
    48001 gttcgttttc ccacaaggag ttggtaaaga gaactaatcc gcagcttgtg tcctgatgaa
    48061 aatagcgata aaataaaaac aatcatatta ttctccttaa gttaggatgg ttagaaatgt
    48121 cactactatg taaacttgta cctgaacgct gtattgcatg cggtctttgt caaatcgaag
    48181 cgccagatat tttcgattat gatgaagaag gaatcgtttt atttgcccaa gaaccagagg
    48241 ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt aaaaaaagcg tatcaaaaat
    48301 gtccagttcg agcgatttta ttagaaaata aaatataaat ataaacgaaa ctttaaaaac
    48361 gtacactaag ttctctaaac ttgaaaataa gccgggatct gtttctcttg ttccgtgaaa
    48421 tcccgactta ttttgaaaga ttcatttgct atctatagtt aattgactag ttttttcacg
    48481 gaccctatag gacccattta tattatttca actttttcag ccagttttcc aatgtattga
    48541 cgatcacagt ttgctggtct gcgttagaaa tttctgcctc tccatctcct ttttgctcac
    48601 catagctgcc aaaacctcca tgatttccgc cattgattga tacatactct gttctttccg
    48661 gtaaatactg cttagcttct tgataagctt cctgattcaa tactttatct tttgtcgccg
    48721 tgatcgataa aacaggaaca gctgattctg tcaatgttcc ttttttgtcg ggataacttg
    48781 ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt cttattgaca taacgacttg
    48841 ccattacgcc acctaatgaa tggccaccga tcacataatc ggaatttgga aaatcttctt
    48901 tgaccttatc cgctttgttt ccgcccatca ctgctagatc aagtggcatt ttgacaatag
    48961 cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat actataactt tctggctcta
    49021 cgagagcacc aggataaaaa ataagtaatg gagactttcc atgattcttt ttctctcctt
    49081 caaaaaataa gtaatcttct ttgttttttc cttcttttac caacacttgc gcttctgacg
    49141 atggttcgta cgtattttgc tgtatatagt aataaccggc tccaccgagt aatcctaaaa
    49201 tgatgactaa gccaattagt attcgtctaa atattttcac tttctcccac cttttctatc
    49261 tgagtgaccc ctttttctta atcaagtatg attatacctt aaaaaaataa gagagaaatg
    49321 caaactttcc agcatcaaat aaaaaaagac ggaatatcca tttgaatatc cgcccttttt
    49381 gtatgtttat ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc
    49441 tttatacata ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc
    49501 agggaacatc gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc
    49561 tgatacaaac ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt
    49621 cactaactca ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt
    49681 caacccatat ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt
    49741 aacagggatt ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc
    49801 aaagttcatc gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc
    49861 agttgcattt tcttgaacta attttcctaa gaaattgatt gggccaaaat caaatacttc
    49921 tgaaagagca attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg
    49981 gatactttta ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt
    50041 tttcaatgct tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac
    50101 aacattcaac gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta
    50161 agcttgcaat aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc
    50221 ttgcgtaatt ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc
    50281 atctgtacca aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc
    50341 tttgacggca tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac
    50401 taaccagccg tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc
    50461 tgctggaaca acgtccatat gacacaagat accgaataat tcatctccag agccaatctc
    50521 tgaataaccg taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat
    50581 ttccaatact ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga
    50641 atctgcttca tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg
    50701 ttcatgatgt tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa
    50761 gggtcatgtc aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct
    50821 gctccatcag caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg
    50881 gagctattga cttatccacg tgcttatcga tagccgttta tcggtttctt cattcttatg
    50941 atagaccttg tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca
    51001 cagatggcta caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca
    51061 tcgaaacgag caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt
    51121 actaaaccag atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat
    51181 tcacccatag gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc
    51241 aagaatgaca tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat
    51301 gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata
    51361 accacttgga taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca
    51421 ccagctaaaa attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg
    51481 atggacatta ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc
    51541 atgcttttac ctagaaccgt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat
    51601 tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata
    51661 ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga
    51721 cgaccgattg cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat
    51781 gattttgttg gatctttttc gatctttgat gcataacgat acacatatac aatagaaata
    51841 gctaacaaaa taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca
    51901 acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact
    51961 tgagacccta ctaatacgat tgctacagct actactgtat caaagcctac agccatcatg
    52021 actgggatca gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg
    52081 cctaatgcaa ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc
    52141 ttcacaactg aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat
    52201 ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc
    52261 tcccccgctg gtacacctag catcccattg attggcgcca tgaaaatatc ccacagccct
    52321 tgaggatttt gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca
    52381 ctatagtgcc cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat
    52441 aagaccgtat acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc
    52501 ttcctttctt ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag
    52561 tatcagaaca aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt
    52621 ccctattccc tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt
    52681 cctttttttt ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa
    52741 agaattgatt gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt
    52801 ttacattatt gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc
    52861 tttttgatat ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta
    52921 accatggaag aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga
    52981 tcaagttgaa aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct
    53041 gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga
    53101 tactcataaa aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc
    53161 ctttatgctg tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg
    53221 ataagaaact agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat
    53281 gtaatactga acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc
    53341 tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg
    53401 gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt
    53461 tctgtacacg ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt
    53521 tttgacgttt ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta
    53581 tttgatcaag cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc
    53641 ggaatttcac cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc
    53701 gggaatttca ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta
    53761 tacatatcta ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga
    53821 gtggttcagc aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag
    53881 gccttgtaaa gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac
    53941 tgcttgaagc atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat
    54001 actgtggttt gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg
    54061 tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt
    54121 tacctttgca acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa
    54181 atctccatca tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg
    54241 atagatacgt tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc
    54301 tgcaacagca gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg
    54361 ataaattggg acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc
    54421 ccttacaact tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg
    54481 acgagaagtg atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct
    54541 cctttattca ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc
    54601 atttcttgtg tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag
    54661 ccagttgttc catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc
    54721 agtttatggg cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg
    54781 atttcagctc ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct
    54841 tgagataatg tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt
    54901 ttagtcgtta atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt
    54961 tgagtgtaag attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt
    55021 aacagtccgc ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct
    55081 tcaagttcac ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga
    55141 ttcatagttt tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc
    55201 cataaaagta gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca
    55261 gccatgcgca tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt
    55321 gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt
    55381 ctgcatggca cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc
    55441 atccgctaac ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc
    55501 tttttctgtc agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac
    55561 caattcttct cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac
    55621 aggaagcaat gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc
    55681 tgcaacagct agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat
    55741 cgtagcgatc ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag
    55801 atcgagagga ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct
    55861 ttcttctttt tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg
    55921 atggagacat ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca
    55981 ccgcgataag acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag
    56041 taaataccaa taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca
    56101 taatcgtctt cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt
    56161 ttcttatgga aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt
    56221 aacatcttct caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc
    56281 attcttttct tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac
    56341 aattcctgta ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc
    56401 cgcagcgtat gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga
    56461 tttttccaga ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt
    56521 tcagatgcgt cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg
    56581 atcgcataat cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc
    56641 agtccaagag aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc
    56701 ccagtagcat acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta
    56761 tcaggtgctt caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt
    56821 gctttcttcg gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt
    56881 aaaaactgat ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt
    56941 ttttcttcat gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat
    57001 gaagcaattt tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt
    57061 tgtcgtgtat tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt
    57121 tgctctttca taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg
    57181 atagtctttt tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt
    57241 ttcttttttc tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt
    57301 ttactaaaaa atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt
    57361 ctacttcctc gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc
    57421 cttcttttct caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt
    57481 tcaattctgt caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct
    57541 tttttttcac taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa
    57601 gcacaagcag agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct
    57661 gttttgtttt tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt
    57721 ataataatta caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag
    57781 ttaagaaaaa catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt
    57841 ttcttgtgta atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt
    57901 gctgatacca aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg
    57961 ctgatctgca gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc
    58021 atatgctctt ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg
    58081 accttttatc tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc
    58141 aagatagaaa tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc
    58201 tatcacatct ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt
    58261 ttcatctgct aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa
    58321 gcgatcccct tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac
    58381 aaagtcttta tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt
    58441 aacgattcca aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt
    58501 ttcatgtttt tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc
    58561 ttctgatttg cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt
    58621 ttcatcaata ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct
    58681 ctatagtgaa aggttttctt aatacatttc tttgctatca taacataatg ttatctactt
    58741 gaatagctga gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata
    58801 ctcccctttt tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta
    58861 aaattaattg ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta
    58921 aaatggcaaa gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata
    58981 tagattataa tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc
    59041 aacgatatat ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca
    59101 cgtccatgct ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg
    59161 attcgctgag tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc
    59221 gaccgcagct tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg
    59281 ttcttcagct gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc
    59341 tttatcagtg agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc
    59401 tgtatttttc actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga
    59461 atccactact tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc
    59521 agcatattga tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt
    59581 atccgtttcg attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg
    59641 atcatcgacg atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt
    59701 agattggata tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact
    59761 ggataaatca ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg
    59821 atggtgatca tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg
    59881 tctttttgct tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca
    59941 gcttcctcca ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt
    60001 tttcttttgc gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc
    60061 agtatctggc acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc
    60121 ttctttttct atttttgctt caagatcagt accctctttg cttacagatt gattttcagc
    60181 atcggattca ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat
    60241 ttcttttttc tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt
    60301 aaatacttca gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt
    60361 acctgtggcg ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac
    60421 tttgttttcc tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc
    60481 ttttgtatct tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt
    60541 atcgacgatt tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc
    60601 ttgattcttt cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt
    60661 ctctatatta gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc
    60721 gaataattcc attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc
    60781 gtttaattct gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc
    60841 tgtatttgtt tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc
    60901 gcagtctgga cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt
    60961 catcacttag cgatcggctc ctttactagg tttacgctac catttaatct atcatattct
    61021 atacgtcacg ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt
    61081 cctatagaaa aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac
    61141 caaacagttt tactgatttc tcttctggtc aatagtccac gcttattcta agaaatcttt
    61201 taattgtttt gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg
    61261 aatacgttcc cgagtaacac caaatacttt ccctacttct tccaatgttc tcgtccggcc
    61321 atcatctaaa ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag
    61381 tacatcttcc aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc
    61441 ttcctggtct tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc
    61501 aagggaaaca ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc
    61561 catttccgca ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg
    61621 ttggatacgt atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg
    61681 tgcttggtca gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga
    61741 aaacttaaat cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc
    61801 ttcttggatc aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac
    61861 tactaaacga aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat
    61921 tttcaatgct aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt
    61981 cagatacata cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt
    62041 ttttgcttgt tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt
    62101 ttcatccacc acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc
    62161 tgcattcaaa gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg
    62221 tttgggtttg ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt
    62281 tccatttgcc ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc
    62341 gttaaattga tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg
    62401 gcttcttgct gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc
    62461 acatctagca agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg
    62521 atttccacca ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg
    62581 ttgaattcat aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca
    62641 tcatgaataa actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat
    62701 gcacgatata atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt
    62761 gcagtttctt gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc
    62821 tgatttgtcc ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac
    62881 tctgttgaca gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt
    62941 tcaactagta attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct
    63001 tgttttaaat aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct
    63061 tggaaagatg cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg
    63121 actacgacag aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt
    63181 gcttcaaaac ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc
    63241 atttgttgga tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata
    63301 ccagcctgcc atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt
    63361 ttccggatat cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt
    63421 tctgtttctg gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca
    63481 agccagcgtc ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact
    63541 ctttgataaa aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt
    63601 tctaacaatt catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca
    63661 ttaggtgcaa aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc
    63721 aaatattcta aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc
    63781 gctgcttttt catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct
    63841 atagtacgct gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc
    63901 actgcttctg gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct
    63961 ttaccacaac caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta
    64021 cgttcttcat gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca
    64081 tattgtccaa tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga
    64141 ggaatccgtt gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga
    64201 agatatttca ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa
    64261 aggacacaat tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca
    64321 tttctatctc attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc
    64381 cttccagtac aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac
    64441 gctctctccc aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata
    64501 tatccctacg atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc
    64561 ttttacaact gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc
    64621 agcatttccc catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac
    64681 aagcaaggca tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt
    64741 tcttttatct tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat
    64801 cagcgtgatt tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga
    64861 taaaataaat gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt
    64921 gaccactcgg cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa
    64981 taccagattc gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc
    65041 tgcttctctt aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc
    65101 gctttctttc aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac
    65161 cgtatagcga tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac
    65221 atacgcatca taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt
    65281 gtctccagaa atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg
    65341 agcgtttttt cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg
    65401 tttttcttcg ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt
    65461 cgcttgggct gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc
    65521 tcctacgtac acgactccca gttctggaaa ttccatcgcg ccccatttgc gatcagaaga
    65581 aaatgcgact ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc
    65641 ccgcatagtg atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat
    65701 ggctttctcg tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt
    65761 aatggtccct gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata
    65821 catgtcttga acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat
    65881 acttattagc aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat
    65941 aacagaaatt ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc
    66001 caaaggaata atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc
    66061 agaacgaata gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc
    66121 acctactcgg ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa
    66181 ttcagctcca gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc
    66241 ttctgcccgt ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt
    66301 taccaaatct cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat
    66361 cactagtact tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat
    66421 ttcttgataa attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga
    66481 atacgcacct acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt
    66541 caatatatta tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt
    66601 ttgctggcca gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt
    66661 tttctccatt ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa
    66721 gattaaaaac aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa
    66781 aagatgccaa cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt
    66841 acttatattg ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa
    66901 gaacaggaca agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca
    66961 taatcgttga agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg
    67021 agaaaatgta atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga
    67081 tcggcacaat cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc
    67141 ccatatacac caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca
    67201 atccgccggc aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag
    67261 cgccgtataa taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag
    67321 aagtatctct tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac
    67381 atccgacaaa aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa
    67441 aagaaagtag aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat
    67501 tttctttgaa attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa
    67561 agacagctgc taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca
    67621 ttcccttcac aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca
    67681 ttttttgcct tttttccact atttgcgtac tccttctttc atttaatttt attcatgatt
    67741 gaacatttcc tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc
    67801 attttgacaa tatcaggata aaacatgtta cctccattat gatagagata gccgccattg
    67861 taaagcaagg actggatccg ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt
    67921 gggctcagca catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga
    67981 ttttttacat aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa
    68041 tcgcatcctt gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca
    68101 atactgtcag ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc
    68161 atgagatctg tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg
    68221 tagttttcca tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact
    68281 cgttttaccg aacgataagt tcttcctcat aaaaacaaca ccgcaccaat aaccgtcaga
    68341 atgatcatgt aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct
    68401 agttattttg acatcttttt ttatcttact atttctttat cttcttggct atttcttcct
    68461 tgatttctgt gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga
    68521 aaatgatcgt tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt
    68581 tacacagtag gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc
    68641 gaaatttact atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac
    68701 ggcagcggcc gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat
    68761 ctagtagata gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag
    68821 ttaatggccg aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt
    68881 cttggattta gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa
    68941 gttcatcgat tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc
    69001 agagtcatca gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt
    69061 cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat
    69121 gatttaaaaa aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag
    69181 ctaaaacatt ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa
    69241 gaacaaggac atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa
    69301 tttctttctg aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat
    69361 acaatggctg aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt
    69421 cattattgtc ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga
    69481 aacgtttgcc actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc
    69541 tgtgttacct atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa
    69601 tcctcgttac ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt
    69661 cggcgggttt gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag
    69721 aaacgtgatc caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta
    69781 cagtctgctt tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata
    69841 ttggatatgg ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt
    69901 caaaaagaaa ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt
    69961 ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga
    70021 aatccggatt gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat
    70081 cgtgtctatg attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc
    70141 cgctattggt gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta
    70201 ctacatcatt ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt
    70261 tgatcagcgt tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt
    70321 ggttgtctta ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc
    70381 ttttacagag tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca
    70441 atttcctagt atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg
    70501 tgtgaaacga gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt
    70561 attaagtgat tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct
    70621 tgcttctaaa aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat
    70681 cgatggaatc attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct
    70741 gccggatttc cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa
    70801 gatttggttt tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt
    70861 tcatttcctt caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct
    70921 gaagatcctt acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa
    70981 gcggatgttt gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt
    71041 caataataaa tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc
    71101 aatcgaagct tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac
    71161 aagcgaagag attgttatga ttttgcatga attcggctac gatttcatta agatggggga
    71221 agaagcttat gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg
    71281 tgctgtcttg aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt
    71341 ttctactgag caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa
    71401 ggaaaaaggg ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc
    71461 agttgtcaaa aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac
    71521 gaacaataaa gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg
    71581 cagtatggat ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg
    71641 gttcaattta gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca
    71701 ggaaaggatt gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt
    71761 aagagaatat aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag
    71821 agatagttgg attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga
    71881 gcagacacaa ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact
    71941 gcataccgat agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt
    72001 catgcttcta caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc
    72061 ctgtttggac cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt
    72121 tcttatgctt cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt
    72181 gagaatgtcg ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg
    72241 ttgtcaccag gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca
    72301 gttgtgcgga agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct
    72361 tcttcttttg tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct
    72421 ggtttagcaa gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg
    72481 cctacgttat ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt
    72541 acaactgctt tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga
    72601 acagcaccac gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg
    72661 tcttccactg gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca
    72721 tctactgtat ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct
    72781 tttaatgcgg aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat
    72841 aactcgcgaa cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact
    72901 ttgttcaaga aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca
    72961 cgtgtttgag gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt
    73021 gctgcccctg taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg
    73081 tagtggcgtt tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca
    73141 cgttcttctg gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg
    73201 cctaatactg tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc
    73261 gtaccaatgt taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa
    73321 cctcctaaat aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt
    73381 ttttgactaa atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt
    73441 attatacgtc aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa
    73501 aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa
    73561 ctttcttttg aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac
    73621 aactttcaat agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg
    73681 aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct
    73741 tatcattatt tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg
    73801 tcagctaccc aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct
    73861 tataataaag atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta
    73921 aagatgaatg cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa
    73981 gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg
    74041 gtcttaaaat tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata
    74101 agaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
    74161 ctttactttt gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga
    74221 cacaattcat ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa
    74281 acgattcaat acttcatcca catttttgat catcacggat atcgtaccat ccggattatt
    74341 cacaaattca atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc
    74401 gtttgatagc ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt
    74461 tacaggaggt gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt
    74521 tgccgtgatg ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt
    74581 ttcttctatc gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt
    74641 ctcaaatttt tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa
    74701 agaaggtgca ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga
    74761 aaactcatat tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc
    74821 ttcatctgct ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc
    74881 gatccctgct tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa
    74941 taaagataag tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg
    75001 tgcttcttca ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc
    75061 cacaaaattt tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg
    75121 tccagttttt gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat
    75181 atattctttt gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc
    75241 aataatatgc agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt
    75301 agtttacgga aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat
    75361 ttcaagagta gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga
    75421 acgaataaaa aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat
    75481 ggttacgaaa tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct
    75541 gtttcctctg ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga
    75601 tacaagaatg aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag
    75661 atattcttga caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct
    75721 tttgaagaat cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg
    75781 ccaaaccctg ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca
    75841 tttgaagaac tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc
    75901 caccatctag atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc
    75961 cttgcaccag gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc
    76021 ctcgaagcat atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa
    76081 atcgctgaag cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac
    76141 gaattccttc ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta
    76201 tttgattttg aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc
    76261 ggcaaagcaa aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc
    76321 aatcgaacgc ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt
    76381 ctttttgatc gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca
    76441 aatcatcgaa gaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga
    76501 acatgaagtc atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa
    76561 agaatggaac ggattatgta tatatacata atccgttcca ttttttattt ttctttcaaa
    76621 aaacgggaaa atacgcaaat ttttctgttg taatttgcgt atacgcaagt ttttttgcat
    76681 tactcctttt catttggctg tttttttctt ataatcgaaa gggtatgagc gaaggagaga
    76741 acatatgaag aaaaaacaga aacaaaaatt attaaatcaa ttccgtcctt cattggatgg
    76801 tgtaagagga caattattcc gttctctaga agaagagagc ttgcgacgtt atggtttgcc
    76861 gttacaagtc atgcttgatc ccaaaaaccg tcaaatctta acgatcggat tagctgggaa
    76921 aacaaatgaa gatacacgga tcaacgtgcc aatcgatgac aacttcaata ctgtattaaa
    76981 gagaattcgt tcaggagaaa acgggatttt cgaacgattc cgtgacaatc ttcttattga
    77041 aatcgtgtct tactggaatg atcaacgatt gaaaaacgat gaaccaactg cgcaagctgt
    77101 tgcgacacca gttacagaag aaacagtcag tgtgccagaa gaaaaaacgg aaaaaccaaa
    77161 agcggacaca actgaaaata cacgctcaac tgatacgaat gccgctttaa gttttgctga
    77221 tttttcaaaa gccgttgctg agtatcctaa attttacaca gaacaaaatg aacatgaagt
    77281 agttattaat gaaaaaaatg ctgatgaacc tcgtaaatta gcaactattt ctatgactac
    77341 tgaaaatgag ttcgttatcg aaaaagcact cgaacggaaa tacaaagtca aattgacttt
    77401 gattcctttg atcgaacaat ttgcagcgac agcgattact gatcgataaa aattctattt
    77461 gtcataaaag gctgggattt aaaatcccag cctttttagt catataataa tttctacagc
    77521 aagtaaaaag aaattccttc taacatcagg ctcaaatatc agcttgacct gtccaacaaa
    77581 tcacctatga tcctacaatg ttaggcgact tctcttctac ctgaagatat cttggagttg
    77641 cctgatttct taagatgcct aaccttaact ataaaaaatt ctaataataa acatattttc
    77701 tgatagagaa aaaa
//
LOCUS       DAYYAG010000002        64887 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000002 DAYYAG010000000
VERSION     DAYYAG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.002"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1421)
                     /locus_tag="ACHARQ_000072"
     CDS             complement(<1..1421)
                     /locus_tag="ACHARQ_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8408533.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="ACHARQ_000073"
     CDS             1673..2854
                     /locus_tag="ACHARQ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8408534.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHARQ_000074"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHARQ_000074"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8408535.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="ACHARQ_000075"
     CDS             complement(4993..7704)
                     /locus_tag="ACHARQ_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8408536.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="ACHARQ_000076"
     CDS             complement(7714..8415)
                     /locus_tag="ACHARQ_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8408537.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="ACHARQ_000077"
     CDS             8601..9200
                     /locus_tag="ACHARQ_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8408538.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="ACHARQ_000078"
     CDS             9281..10294
                     /locus_tag="ACHARQ_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8408539.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHARQ_000079"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHARQ_000079"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8408540.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHARQ_000080"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHARQ_000080"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8408541.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="ACHARQ_000081"
     CDS             11742..13115
                     /locus_tag="ACHARQ_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8408542.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="ACHARQ_000082"
     CDS             13472..15616
                     /locus_tag="ACHARQ_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8408543.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="ACHARQ_000083"
     CDS             complement(15668..16708)
                     /locus_tag="ACHARQ_000083"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8408544.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="ACHARQ_000084"
     CDS             complement(16985..18787)
                     /locus_tag="ACHARQ_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8408545.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="ACHARQ_000085"
     CDS             complement(18801..19553)
                     /locus_tag="ACHARQ_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8408546.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="ACHARQ_000086"
     CDS             complement(19562..19924)
                     /locus_tag="ACHARQ_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8408547.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="ACHARQ_000087"
     CDS             complement(20100..20972)
                     /locus_tag="ACHARQ_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8408548.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="ACHARQ_000088"
     CDS             complement(21023..21511)
                     /locus_tag="ACHARQ_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8408549.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22440)
                     /locus_tag="ACHARQ_000089"
     CDS             complement(21649..22440)
                     /locus_tag="ACHARQ_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8408550.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22495..23103)
                     /locus_tag="ACHARQ_000090"
     CDS             complement(22495..23103)
                     /locus_tag="ACHARQ_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8408551.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="ACHARQ_000091"
     CDS             complement(23096..23413)
                     /locus_tag="ACHARQ_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8408552.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="ACHARQ_000092"
     CDS             complement(23569..24297)
                     /locus_tag="ACHARQ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8408553.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="ACHARQ_000093"
     CDS             24456..25337
                     /locus_tag="ACHARQ_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8408554.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="ACHARQ_000094"
     CDS             complement(25574..26617)
                     /locus_tag="ACHARQ_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8408555.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="ACHARQ_000095"
     CDS             complement(26620..27288)
                     /locus_tag="ACHARQ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8408556.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="ACHARQ_000096"
     CDS             27430..29766
                     /locus_tag="ACHARQ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8408557.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="ACHARQ_000097"
     CDS             complement(29881..30192)
                     /locus_tag="ACHARQ_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408558.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="ACHARQ_000098"
     CDS             30373..31848
                     /locus_tag="ACHARQ_000098"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8408559.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="ACHARQ_000099"
     CDS             31895..32479
                     /locus_tag="ACHARQ_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8408560.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="ACHARQ_000100"
     CDS             32481..32864
                     /locus_tag="ACHARQ_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8408561.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="ACHARQ_000101"
     CDS             complement(33181..33465)
                     /locus_tag="ACHARQ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8408562.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="ACHARQ_000102"
     CDS             complement(33817..34527)
                     /locus_tag="ACHARQ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8408563.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="ACHARQ_000103"
     CDS             complement(34524..35996)
                     /locus_tag="ACHARQ_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8408564.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="ACHARQ_000104"
     CDS             36134..36607
                     /locus_tag="ACHARQ_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8408565.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="ACHARQ_000105"
     CDS             complement(36760..37311)
                     /locus_tag="ACHARQ_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8408566.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="ACHARQ_000106"
     CDS             37416..37946
                     /locus_tag="ACHARQ_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8408567.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="ACHARQ_000107"
     CDS             complement(38429..38872)
                     /locus_tag="ACHARQ_000107"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8408568.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="ACHARQ_000108"
     CDS             complement(38888..40048)
                     /locus_tag="ACHARQ_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8408569.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="ACHARQ_000109"
     CDS             40555..42255
                     /locus_tag="ACHARQ_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408570.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="ACHARQ_000110"
     CDS             complement(42357..43190)
                     /locus_tag="ACHARQ_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8408571.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="ACHARQ_000111"
     CDS             complement(43494..45659)
                     /locus_tag="ACHARQ_000111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8408572.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="ACHARQ_000112"
     CDS             45919..48213
                     /locus_tag="ACHARQ_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8408573.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="ACHARQ_000113"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="ACHARQ_000113"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8408574.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="ACHARQ_000114"
     CDS             48864..49871
                     /locus_tag="ACHARQ_000114"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8408575.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="ACHARQ_000115"
     CDS             49893..50912
                     /locus_tag="ACHARQ_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8408576.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="ACHARQ_000116"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="ACHARQ_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8408577.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /gene="tyrP"
                     /locus_tag="ACHARQ_000117"
     CDS             complement(52669..54090)
                     /gene="tyrP"
                     /locus_tag="ACHARQ_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8408578.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="ACHARQ_000118"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="ACHARQ_000118"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8408579.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /gene="tyrS"
                     /locus_tag="ACHARQ_000119"
     CDS             complement(56501..57757)
                     /gene="tyrS"
                     /locus_tag="ACHARQ_000119"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8408580.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57869..58074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58337..59638
                     /locus_tag="ACHARQ_000120"
     CDS             58337..59638
                     /locus_tag="ACHARQ_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8408581.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="ACHARQ_000121"
     CDS             complement(59707..60306)
                     /locus_tag="ACHARQ_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8408582.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60560..61441
                     /locus_tag="ACHARQ_000122"
     CDS             60560..61441
                     /locus_tag="ACHARQ_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8408583.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61454..63064
                     /locus_tag="ACHARQ_000123"
     CDS             61454..63064
                     /locus_tag="ACHARQ_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8408584.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63141..63800)
                     /locus_tag="ACHARQ_000124"
     CDS             complement(63141..63800)
                     /locus_tag="ACHARQ_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8408585.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64083..64748)
                     /locus_tag="ACHARQ_000125"
     CDS             complement(64083..64748)
                     /locus_tag="ACHARQ_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8408586.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        19457 a        13218 c        11704 g        20508 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60361 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60421 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60481 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60541 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60601 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60661 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60721 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60781 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60841 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60901 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    60961 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61021 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61081 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61141 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag aaggaacact
    61201 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61261 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61321 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61381 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61441 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61501 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61561 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61621 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61681 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61741 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61801 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61861 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61921 agcggattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    61981 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62041 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62101 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62161 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62221 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62281 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62341 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62401 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62461 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtag tcttgatttg
    62521 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62581 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62641 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62701 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62761 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62821 atggcttcct cgaatcggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62881 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    62941 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63001 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63061 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63121 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63181 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63241 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63301 tgatccttcc ctgtcgcttt agctaagtca agcagataga gagcgactcg ttcttctact
    63361 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63421 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63481 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63541 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63601 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63661 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63721 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63781 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63841 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63901 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    63961 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64021 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64081 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64141 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64201 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64261 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64321 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64381 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64441 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64501 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64561 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64621 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64681 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64741 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64801 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64861 aactgcgatg agcatcaccg cagtttt
//
LOCUS       DAYYAG010000003        61258 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000003 DAYYAG010000000
VERSION     DAYYAG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.003"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACHARQ_000126"
     CDS             complement(122..499)
                     /locus_tag="ACHARQ_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408587.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /gene="purC"
                     /locus_tag="ACHARQ_000127"
     CDS             755..1483
                     /gene="purC"
                     /locus_tag="ACHARQ_000127"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8408588.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="ACHARQ_000128"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="ACHARQ_000128"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8408589.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="ACHARQ_000129"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="ACHARQ_000129"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8408590.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="ACHARQ_000130"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="ACHARQ_000130"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8408591.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /gene="purF"
                     /locus_tag="ACHARQ_000131"
     CDS             4618..6057
                     /gene="purF"
                     /locus_tag="ACHARQ_000131"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8408592.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /gene="purM"
                     /locus_tag="ACHARQ_000132"
     CDS             6080..7132
                     /gene="purM"
                     /locus_tag="ACHARQ_000132"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8408593.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="ACHARQ_000133"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="ACHARQ_000133"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8408594.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="ACHARQ_000134"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="ACHARQ_000134"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8408595.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="ACHARQ_000135"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="ACHARQ_000135"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8408596.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="ACHARQ_000136"
     CDS             10651..11565
                     /locus_tag="ACHARQ_000136"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8408597.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="ACHARQ_000137"
     CDS             complement(11698..13263)
                     /locus_tag="ACHARQ_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8408598.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="ACHARQ_000138"
     CDS             complement(13256..14707)
                     /locus_tag="ACHARQ_000138"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8408599.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="ACHARQ_000139"
     CDS             complement(14721..15737)
                     /locus_tag="ACHARQ_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8408600.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /gene="chvE"
                     /locus_tag="ACHARQ_000140"
     CDS             15957..17045
                     /gene="chvE"
                     /locus_tag="ACHARQ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8408601.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /gene="mmsA"
                     /locus_tag="ACHARQ_000141"
     CDS             17099..18625
                     /gene="mmsA"
                     /locus_tag="ACHARQ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8408602.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /gene="mmsB"
                     /locus_tag="ACHARQ_000142"
     CDS             18627..19832
                     /gene="mmsB"
                     /locus_tag="ACHARQ_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8408603.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="ACHARQ_000143"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="ACHARQ_000143"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8408604.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="ACHARQ_000144"
     CDS             21330..22754
                     /locus_tag="ACHARQ_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8408605.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22796..23053)
                     /locus_tag="ACHARQ_000145"
     CDS             complement(22796..23053)
                     /locus_tag="ACHARQ_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408606.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="ACHARQ_000146"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="ACHARQ_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8408607.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="ACHARQ_000147"
     CDS             24327..25304
                     /locus_tag="ACHARQ_000147"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8408608.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="ACHARQ_000148"
     CDS             25329..26972
                     /locus_tag="ACHARQ_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8408609.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="ACHARQ_000149"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="ACHARQ_000149"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8408610.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="ACHARQ_000150"
     CDS             28548..28823
                     /locus_tag="ACHARQ_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8408611.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="ACHARQ_000151"
     CDS             28829..29596
                     /locus_tag="ACHARQ_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8408612.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="ACHARQ_000152"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="ACHARQ_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8408613.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="ACHARQ_000153"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="ACHARQ_000153"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8408614.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="ACHARQ_000154"
     CDS             33740..34042
                     /locus_tag="ACHARQ_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8408615.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="ACHARQ_000155"
     CDS             34045..35208
                     /locus_tag="ACHARQ_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8408616.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="ACHARQ_000156"
     CDS             35234..38662
                     /locus_tag="ACHARQ_000156"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8408617.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="ACHARQ_000157"
     CDS             38683..39807
                     /locus_tag="ACHARQ_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8408618.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39788..40234
                     /locus_tag="ACHARQ_000158"
     CDS             39788..40234
                     /locus_tag="ACHARQ_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8408619.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="ACHARQ_000159"
     CDS             40218..40556
                     /locus_tag="ACHARQ_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8408620.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="ACHARQ_000160"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="ACHARQ_000160"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8408621.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="ACHARQ_000161"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="ACHARQ_000161"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8408622.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="ACHARQ_000162"
     CDS             41713..42756
                     /locus_tag="ACHARQ_000162"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8408623.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="ACHARQ_000163"
     CDS             42915..43643
                     /locus_tag="ACHARQ_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8408624.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="ACHARQ_000164"
     CDS             43759..44004
                     /locus_tag="ACHARQ_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8408625.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="ACHARQ_000165"
     CDS             44001..44219
                     /locus_tag="ACHARQ_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8408626.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="ACHARQ_000166"
     CDS             44355..44894
                     /locus_tag="ACHARQ_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8408627.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="ACHARQ_000167"
     CDS             44887..47160
                     /locus_tag="ACHARQ_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8408628.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="ACHARQ_000168"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="ACHARQ_000168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8408629.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /gene="rpsT"
                     /locus_tag="ACHARQ_000169"
     CDS             complement(48333..48587)
                     /gene="rpsT"
                     /locus_tag="ACHARQ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8408630.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="ACHARQ_000170"
     CDS             complement(48987..49256)
                     /locus_tag="ACHARQ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408631.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49518..49646)
                     /locus_tag="ACHARQ_000171"
     CDS             complement(49518..49646)
                     /locus_tag="ACHARQ_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408632.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49726..50181)
                     /locus_tag="ACHARQ_000172"
     CDS             complement(49726..50181)
                     /locus_tag="ACHARQ_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8408633.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="ACHARQ_000173"
     CDS             complement(50319..52247)
                     /locus_tag="ACHARQ_000173"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8408634.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52444..53277)
                     /locus_tag="ACHARQ_000174"
     CDS             complement(52444..53277)
                     /locus_tag="ACHARQ_000174"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8408635.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53469..54173)
                     /locus_tag="ACHARQ_000175"
     CDS             complement(53469..54173)
                     /locus_tag="ACHARQ_000175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8408636.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54196..54822)
                     /locus_tag="ACHARQ_000176"
     CDS             complement(54196..54822)
                     /locus_tag="ACHARQ_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8408637.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55148..57523
                     /locus_tag="ACHARQ_000177"
     CDS             55148..57523
                     /locus_tag="ACHARQ_000177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8408638.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57547..58038)
                     /locus_tag="ACHARQ_000178"
     CDS             complement(57547..58038)
                     /locus_tag="ACHARQ_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8408639.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58225..59145
                     /locus_tag="ACHARQ_000179"
     CDS             58225..59145
                     /locus_tag="ACHARQ_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8408640.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59299..59643
                     /locus_tag="ACHARQ_000180"
     CDS             59299..59643
                     /locus_tag="ACHARQ_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8408641.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59833..60333)
                     /locus_tag="ACHARQ_000181"
     CDS             complement(59833..60333)
                     /locus_tag="ACHARQ_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8408642.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60861..60944
                     /locus_tag="ACHARQ_000182"
     tRNA            60861..60944
                     /locus_tag="ACHARQ_000182"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60895..60897,aa:Leu,seq:cag)
BASE COUNT        20140 a        10570 c        13107 g        17441 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    52381 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    52441 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    52501 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    52561 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    52621 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    52681 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    52741 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    52801 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    52861 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    52921 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    52981 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    53041 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    53101 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    53161 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    53221 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    53281 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    53341 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    53401 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    53461 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    53521 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    53581 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    53641 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    53701 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    53761 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    53821 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    53881 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    53941 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    54001 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    54061 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    54121 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    54181 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    54241 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    54301 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    54361 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    54421 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    54481 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    54541 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    54601 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    54661 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    54721 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    54781 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    54841 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    54901 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    54961 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    55021 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    55081 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    55141 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    55201 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    55261 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    55321 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    55381 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    55441 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    55501 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    55561 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    55621 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    55681 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    55741 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    55801 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    55861 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    55921 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    55981 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    56041 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    56101 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    56161 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    56221 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    56281 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    56341 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    56401 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    56461 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    56521 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    56581 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    56641 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    56701 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    56761 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    56821 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    56881 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    56941 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    57001 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    57061 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    57121 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    57181 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    57241 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    57301 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    57361 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    57421 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    57481 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    57541 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    57601 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    57661 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    57721 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    57781 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    57841 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    57901 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    57961 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    58021 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    58081 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    58141 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    58201 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    58261 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    58321 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    58381 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    58441 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    58501 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    58561 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    58621 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    58681 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    58741 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    58801 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    58861 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    58921 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    58981 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    59041 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    59101 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    59161 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    59221 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    59281 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    59341 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    59401 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    59461 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    59521 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    59581 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    59641 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    59701 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    59761 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    59821 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    59881 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    59941 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    60001 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    60061 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    60121 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    60181 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    60241 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    60301 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    60361 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    60421 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    60481 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    60541 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    60601 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    60661 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    60721 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    60781 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    60841 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    60901 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    60961 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    61021 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    61081 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    61141 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    61201 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DAYYAG010000004        56537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000004 DAYYAG010000000
VERSION     DAYYAG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.004"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..1673
                     /locus_tag="ACHARQ_000183"
     CDS             42..1673
                     /locus_tag="ACHARQ_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8408643.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1735..2376)
                     /locus_tag="ACHARQ_000184"
     CDS             complement(1735..2376)
                     /locus_tag="ACHARQ_000184"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8408644.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2562..2638
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2658..2966
                     /gene="rplU"
                     /locus_tag="ACHARQ_000185"
     CDS             2658..2966
                     /gene="rplU"
                     /locus_tag="ACHARQ_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8408645.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2993..3337
                     /locus_tag="ACHARQ_000186"
     CDS             2993..3337
                     /locus_tag="ACHARQ_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8408646.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3350..3643
                     /gene="rpmA"
                     /locus_tag="ACHARQ_000187"
     CDS             3350..3643
                     /gene="rpmA"
                     /locus_tag="ACHARQ_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8408647.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4077..6884
                     /locus_tag="ACHARQ_000188"
     CDS             4077..6884
                     /locus_tag="ACHARQ_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8408648.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7075..7566
                     /locus_tag="ACHARQ_000189"
     CDS             7075..7566
                     /locus_tag="ACHARQ_000189"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8408649.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7669..8658
                     /locus_tag="ACHARQ_000190"
     CDS             7669..8658
                     /locus_tag="ACHARQ_000190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8408650.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8691..9494
                     /locus_tag="ACHARQ_000191"
     CDS             8691..9494
                     /locus_tag="ACHARQ_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8408651.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9515..10429
                     /locus_tag="ACHARQ_000192"
     CDS             9515..10429
                     /locus_tag="ACHARQ_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8408652.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10512..10886
                     /locus_tag="ACHARQ_000193"
     CDS             10512..10886
                     /locus_tag="ACHARQ_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8408653.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11025..11270
                     /locus_tag="ACHARQ_000194"
     CDS             11025..11270
                     /locus_tag="ACHARQ_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8408654.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11442..12506
                     /locus_tag="ACHARQ_000195"
     CDS             11442..12506
                     /locus_tag="ACHARQ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8408655.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12752..13177
                     /locus_tag="ACHARQ_000196"
     CDS             12752..13177
                     /locus_tag="ACHARQ_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8408656.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13170..13619
                     /gene="nusB"
                     /locus_tag="ACHARQ_000197"
     CDS             13170..13619
                     /gene="nusB"
                     /locus_tag="ACHARQ_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8408657.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13716..14570
                     /locus_tag="ACHARQ_000198"
     CDS             13716..14570
                     /locus_tag="ACHARQ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8408658.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14571..15908
                     /gene="xseA"
                     /locus_tag="ACHARQ_000199"
     CDS             14571..15908
                     /gene="xseA"
                     /locus_tag="ACHARQ_000199"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8408659.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15913..16137
                     /locus_tag="ACHARQ_000200"
     CDS             15913..16137
                     /locus_tag="ACHARQ_000200"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8408660.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16139..17029
                     /locus_tag="ACHARQ_000201"
     CDS             16139..17029
                     /locus_tag="ACHARQ_000201"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8408661.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17038..17856
                     /locus_tag="ACHARQ_000202"
     CDS             17038..17856
                     /locus_tag="ACHARQ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8408662.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17963..18412
                     /locus_tag="ACHARQ_000203"
     CDS             17963..18412
                     /locus_tag="ACHARQ_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8408663.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18430..20112
                     /gene="recN"
                     /locus_tag="ACHARQ_000204"
     CDS             18430..20112
                     /gene="recN"
                     /locus_tag="ACHARQ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8408664.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20167..20283)
                     /locus_tag="ACHARQ_000205"
     CDS             complement(20167..20283)
                     /locus_tag="ACHARQ_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8408665.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20356..21312)
                     /locus_tag="ACHARQ_000206"
     CDS             complement(20356..21312)
                     /locus_tag="ACHARQ_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8408666.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21483..21851
                     /locus_tag="ACHARQ_000207"
     CDS             21483..21851
                     /locus_tag="ACHARQ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8408667.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22021..22452
                     /gene="mraZ"
                     /locus_tag="ACHARQ_000208"
     CDS             22021..22452
                     /gene="mraZ"
                     /locus_tag="ACHARQ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8408668.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22473..23432
                     /gene="rsmH"
                     /locus_tag="ACHARQ_000209"
     CDS             22473..23432
                     /gene="rsmH"
                     /locus_tag="ACHARQ_000209"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8408669.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23437..23838
                     /gene="ftsL"
                     /locus_tag="ACHARQ_000210"
     CDS             23437..23838
                     /gene="ftsL"
                     /locus_tag="ACHARQ_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8408670.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23839..26031
                     /locus_tag="ACHARQ_000211"
     CDS             23839..26031
                     /locus_tag="ACHARQ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8408671.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26057..27019
                     /gene="mraY"
                     /locus_tag="ACHARQ_000212"
     CDS             26057..27019
                     /gene="mraY"
                     /locus_tag="ACHARQ_000212"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8408672.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27033..28394
                     /gene="murD"
                     /locus_tag="ACHARQ_000213"
     CDS             27033..28394
                     /gene="murD"
                     /locus_tag="ACHARQ_000213"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8408673.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28420..29508
                     /gene="murG"
                     /locus_tag="ACHARQ_000214"
     CDS             28420..29508
                     /gene="murG"
                     /locus_tag="ACHARQ_000214"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8408674.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29523..30743
                     /locus_tag="ACHARQ_000215"
     CDS             29523..30743
                     /locus_tag="ACHARQ_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8408675.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30899..32224
                     /gene="ftsA"
                     /locus_tag="ACHARQ_000216"
     CDS             30899..32224
                     /gene="ftsA"
                     /locus_tag="ACHARQ_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8408676.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32247..33488
                     /gene="ftsZ"
                     /locus_tag="ACHARQ_000217"
     CDS             32247..33488
                     /gene="ftsZ"
                     /locus_tag="ACHARQ_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8408677.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33505..34182
                     /locus_tag="ACHARQ_000218"
     CDS             33505..34182
                     /locus_tag="ACHARQ_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8408678.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34196..34795
                     /locus_tag="ACHARQ_000219"
     CDS             34196..34795
                     /locus_tag="ACHARQ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8408679.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34876..35097
                     /locus_tag="ACHARQ_000220"
     CDS             34876..35097
                     /locus_tag="ACHARQ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8408680.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35146..35928
                     /locus_tag="ACHARQ_000221"
     CDS             35146..35928
                     /locus_tag="ACHARQ_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8408681.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35949..36656
                     /locus_tag="ACHARQ_000222"
     CDS             35949..36656
                     /locus_tag="ACHARQ_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8408682.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36918..39716
                     /gene="ileS"
                     /locus_tag="ACHARQ_000223"
     CDS             36918..39716
                     /gene="ileS"
                     /locus_tag="ACHARQ_000223"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8408683.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39765..41291)
                     /gene="zwf"
                     /locus_tag="ACHARQ_000224"
     CDS             complement(39765..41291)
                     /gene="zwf"
                     /locus_tag="ACHARQ_000224"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8408684.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41306..41953)
                     /locus_tag="ACHARQ_000225"
     CDS             complement(41306..41953)
                     /locus_tag="ACHARQ_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8408685.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42137..42466)
                     /locus_tag="ACHARQ_000226"
     CDS             complement(42137..42466)
                     /locus_tag="ACHARQ_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8408686.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42643..43371
                     /gene="sfsA"
                     /locus_tag="ACHARQ_000227"
     CDS             42643..43371
                     /gene="sfsA"
                     /locus_tag="ACHARQ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8408687.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43387..44400
                     /locus_tag="ACHARQ_000228"
     CDS             43387..44400
                     /locus_tag="ACHARQ_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8408688.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44400..45677
                     /locus_tag="ACHARQ_000229"
     CDS             44400..45677
                     /locus_tag="ACHARQ_000229"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8408689.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45740..48442
                     /locus_tag="ACHARQ_000230"
     CDS             45740..48442
                     /locus_tag="ACHARQ_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8408690.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48594..48911
                     /locus_tag="ACHARQ_000231"
     CDS             48594..48911
                     /locus_tag="ACHARQ_000231"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8408691.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48941..49261
                     /locus_tag="ACHARQ_000232"
     CDS             48941..49261
                     /locus_tag="ACHARQ_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8408692.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49369..50829
                     /locus_tag="ACHARQ_000233"
     CDS             49369..50829
                     /locus_tag="ACHARQ_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8408693.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50897..51118
                     /locus_tag="ACHARQ_000234"
     CDS             50897..51118
                     /locus_tag="ACHARQ_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408694.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51149..51331
                     /locus_tag="ACHARQ_000235"
     CDS             51149..51331
                     /locus_tag="ACHARQ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8408695.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51331..51744
                     /locus_tag="ACHARQ_000236"
     CDS             51331..51744
                     /locus_tag="ACHARQ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8408696.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51867..53048
                     /locus_tag="ACHARQ_000237"
     CDS             51867..53048
                     /locus_tag="ACHARQ_000237"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8408697.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53119..53283)
                     /locus_tag="ACHARQ_000238"
     CDS             complement(53119..53283)
                     /locus_tag="ACHARQ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408698.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53437..53510
                     /locus_tag="ACHARQ_000239"
     tRNA            53437..53510
                     /locus_tag="ACHARQ_000239"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53471..53473,aa:Arg,seq:cct)
     gene            complement(53579..54718)
                     /locus_tag="ACHARQ_000240"
     CDS             complement(53579..54718)
                     /locus_tag="ACHARQ_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8408699.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            54890..55024
                     /locus_tag="ACHARQ_000241"
     CDS             54890..55024
                     /locus_tag="ACHARQ_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408700.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(55017..55652)
                     /locus_tag="ACHARQ_000242"
     CDS             complement(55017..55652)
                     /locus_tag="ACHARQ_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408701.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(55765..56400)
                     /locus_tag="ACHARQ_000243"
     CDS             complement(55765..56400)
                     /locus_tag="ACHARQ_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8408702.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(56434..>56537)
                     /locus_tag="ACHARQ_000244"
     CDS             complement(56434..>56537)
                     /locus_tag="ACHARQ_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8408703.1"
                     /translation="SSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        18545 a         9590 c        12029 g        16373 t
ORIGIN      
        1 aatggtattt tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt
       61 cctcactaaa aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg
      121 ctgggatcac agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg
      181 gagctgatgc ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt
      241 tatcaggcgg tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt
      301 ctattgcggc aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat
      361 ttttacttgc agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg
      421 taattacggg gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt
      481 tatttggtgt tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga
      541 taggatttcc gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt
      601 ttatctatcc aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg
      661 caacagcagt tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa
      721 cattgatcag tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag
      781 tggcagttcc agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg
      841 cttcggctgg ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag
      901 gaattggaaa tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc
      961 gtacaagcgt ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag
     1021 ttgtcttact gctctccatg cttgtttttg cgccagtgat gtcacagttt ccaatgccag
     1081 cacttgcagg tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg
     1141 agctatttac taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta
     1201 cagttatctt tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt
     1261 ttgttgtcaa aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg
     1321 ggctgccaga aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt
     1381 tttttatgtc ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg
     1441 tcattttctc catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg
     1501 aatttcaaga aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag
     1561 aagtagaaaa acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag
     1621 ttgctcatgc gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg
     1681 aaaaagaaca gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata
     1741 ttgctgtaaa agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg
     1801 attgtagaaa atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca
     1861 ttcgtaggca tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg
     1921 atttttggta accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt
     1981 cgcaattaat tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa
     2041 tacttcggaa cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt
     2101 attgatccga tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc
     2161 ttctcgtgtt aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct
     2221 aaaaggaaca tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt
     2281 aggatgaaga ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa
     2341 agaagcttct tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat
     2401 tataagaata aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa
     2461 agcagaagta ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg
     2521 gttgctgttc tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca
     2581 gaacaagtaa taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga
     2641 aggaggtgct ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg
     2701 aagtgggaca agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt
     2761 ttgacgaagt tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag
     2821 gtgcaactgt tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca
     2881 aatacaaacc taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag
     2941 tggttatcga cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa
     3001 aggaacattt aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc
     3061 tgatgctggt ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag
     3121 cactgtcaac gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga
     3181 agaaggcggt tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat
     3241 tgctgagatt ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc
     3301 agaatatatc caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc
     3361 tatgaatcta caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga
     3421 ctcagaatca aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc
     3481 aattctttac cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg
     3541 agacgacact ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa
     3601 caaaaaacaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     3661 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     3721 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     3781 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     3841 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     3901 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     3961 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     4021 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     4081 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     4141 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     4201 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     4261 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     4321 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     4381 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     4441 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     4501 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     4561 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     4621 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     4681 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     4741 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     4801 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     4861 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     4921 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     4981 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     5041 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     5101 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     5161 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     5221 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     5281 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     5341 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     5401 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     5461 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     5521 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     5581 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     5641 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     5701 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     5761 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     5821 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     5881 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     5941 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     6001 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     6061 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     6121 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     6181 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     6241 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     6301 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     6361 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     6421 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     6481 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     6541 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     6601 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     6661 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     6721 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     6781 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     6841 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     6901 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     6961 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     7021 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     7081 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     7141 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     7201 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     7261 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     7321 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     7381 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     7441 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     7501 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     7561 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
     7621 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
     7681 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
     7741 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
     7801 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
     7861 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
     7921 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
     7981 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
     8041 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
     8101 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
     8161 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
     8221 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
     8281 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
     8341 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
     8401 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
     8461 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
     8521 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
     8581 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
     8641 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
     8701 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
     8761 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
     8821 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
     8881 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
     8941 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
     9001 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
     9061 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
     9121 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
     9181 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
     9241 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
     9301 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
     9361 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
     9421 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
     9481 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
     9541 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
     9601 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
     9661 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
     9721 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
     9781 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
     9841 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
     9901 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
     9961 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
    10021 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
    10081 agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt
    10141 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
    10201 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
    10261 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
    10321 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
    10381 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
    10441 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
    10501 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
    10561 cgtttacggg tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt
    10621 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
    10681 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
    10741 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
    10801 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
    10861 ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt
    10921 tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta
    10981 aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga
    11041 cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt
    11101 cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga
    11161 ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct attttaggcg
    11221 cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa
    11281 aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta
    11341 aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc
    11401 tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa
    11461 aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca
    11521 atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa
    11581 aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt
    11641 ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag
    11701 aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt
    11761 tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag
    11821 aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg
    11881 catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc
    11941 aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg
    12001 ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag
    12061 gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac
    12121 gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag
    12181 atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg
    12241 cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag
    12301 gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc
    12361 aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg
    12421 gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt
    12481 caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag
    12541 tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt
    12601 taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga
    12661 tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat
    12721 ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa
    12781 cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg
    12841 gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt
    12901 cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga
    12961 tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc
    13021 aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc
    13081 tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga
    13141 agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc
    13201 gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag
    13261 atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag
    13321 agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat
    13381 tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa
    13441 tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag
    13501 cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac
    13561 gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag
    13621 tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta
    13681 gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag
    13741 aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg
    13801 gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg
    13861 taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc
    13921 ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta
    13981 gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc
    14041 tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac
    14101 tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg
    14161 aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg
    14221 tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt
    14281 ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag
    14341 gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag
    14401 ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc
    14461 ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc
    14521 tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag
    14581 aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt
    14641 atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc
    14701 atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag
    14761 cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta
    14821 tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg
    14881 gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc
    14941 tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca
    15001 gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg
    15061 tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca
    15121 ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg
    15181 gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc
    15241 aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt
    15301 tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt
    15361 tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac
    15421 gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc
    15481 agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat
    15541 atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc
    15601 aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc
    15661 tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg
    15721 ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca
    15781 gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt
    15841 tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag
    15901 gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt
    15961 gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga
    16021 ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg
    16081 aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat
    16141 gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt
    16201 tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc
    16261 aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc
    16321 tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt
    16381 gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa
    16441 tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc
    16501 attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca
    16561 attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg
    16621 cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca
    16681 tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt
    16741 attgctactt caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct
    16801 actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat
    16861 ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat
    16921 tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga
    16981 tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg
    17041 gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa
    17101 gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa
    17161 ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat
    17221 gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa
    17281 ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa
    17341 aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata
    17401 agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa
    17461 gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa
    17521 ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc
    17581 aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct
    17641 cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat
    17701 gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct
    17761 cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg
    17821 gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga
    17881 gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa
    17941 tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt
    18001 gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg
    18061 aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt
    18121 accggcttca gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa
    18181 tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt
    18241 tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg
    18301 ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg
    18361 aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga
    18421 gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg
    18481 ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt
    18541 attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa
    18601 ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct
    18661 gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat
    18721 atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat
    18781 ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg
    18841 ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag
    18901 aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga
    18961 aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa
    19021 gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga
    19081 gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg
    19141 acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg
    19201 atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat
    19261 ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag
    19321 agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac
    19381 ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct
    19441 tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt
    19501 ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg
    19561 gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta
    19621 cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt
    19681 tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc
    19741 gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca
    19801 agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca
    19861 gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa
    19921 gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg
    19981 caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg
    20041 gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca
    20101 aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt
    20161 ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag
    20221 cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg
    20281 cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc
    20341 cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca
    20401 aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa
    20461 cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa
    20521 agaagttaaa attttcataa tattattcag attattcgat acgattgctg aaaaagtctg
    20581 attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc
    20641 caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg
    20701 cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct
    20761 tttttggata tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc
    20821 ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac
    20881 ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt
    20941 ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt
    21001 tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact
    21061 tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt
    21121 gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg
    21181 aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata
    21241 ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa
    21301 atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt
    21361 ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg
    21421 aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt
    21481 tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt
    21541 ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc
    21601 ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga
    21661 caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg
    21721 ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc
    21781 gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt
    21841 cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt
    21901 gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg
    21961 gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca
    22021 atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca
    22081 aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt
    22141 ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg
    22201 gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa
    22261 cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca
    22321 aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg
    22381 caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat
    22441 ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa cactatacag
    22501 tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact
    22561 gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc
    22621 atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt
    22681 cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac
    22741 gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc
    22801 ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc
    22861 gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg
    22921 aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg
    22981 aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca
    23041 ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt
    23101 ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac
    23161 aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg
    23221 aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg
    23281 gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa
    23341 tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta
    23401 ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa
    23461 gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct
    23521 tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct
    23581 gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta
    23641 acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca
    23701 gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct
    23761 cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat
    23821 ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa
    23881 aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt
    23941 ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct
    24001 ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca
    24061 aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat
    24121 tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa
    24181 gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa
    24241 gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt
    24301 agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa
    24361 gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc
    24421 ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt
    24481 aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt
    24541 tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct
    24601 gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca
    24661 ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa
    24721 gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg
    24781 gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa
    24841 ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac
    24901 gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat
    24961 gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc
    25021 gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg
    25081 ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca
    25141 ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc
    25201 caaatgatga aagctttcac ttctattgcg aataacggta caatgattca acctcgatat
    25261 atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta
    25321 gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc
    25381 gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg
    25441 gcaaaaaacg gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca
    25501 gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat
    25561 ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca
    25621 ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa
    25681 acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat
    25741 gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa
    25801 tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt
    25861 gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa
    25921 atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct
    25981 ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa
    26041 taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc
    26101 aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca
    26161 ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg
    26221 cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa
    26281 acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg
    26341 ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca
    26401 gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg
    26461 ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat
    26521 tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga
    26581 tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca
    26641 tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt
    26701 cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt
    26761 gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat
    26821 tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct
    26881 tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg
    26941 gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac
    27001 attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata
    27061 agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg
    27121 aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac
    27181 aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg
    27241 atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag
    27301 caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag
    27361 cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga
    27421 caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg
    27481 ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat
    27541 tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca
    27601 atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat
    27661 ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag
    27721 aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag
    27781 tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa
    27841 tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta
    27901 tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt
    27961 tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata
    28021 atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt
    28081 ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc
    28141 cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg
    28201 atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag
    28261 ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg
    28321 ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga
    28381 accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc
    28441 ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa
    28501 gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc
    28561 cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc
    28621 atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc
    28681 ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta
    28741 gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca
    28801 ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat
    28861 gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa
    28921 gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa
    28981 acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt
    29041 tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat
    29101 tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat
    29161 atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca
    29221 acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat
    29281 gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa
    29341 atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg
    29401 ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat
    29461 gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct
    29521 tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt
    29581 tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag
    29641 agccaaaaaa agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa
    29701 cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag
    29761 agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca
    29821 atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat
    29881 tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag
    29941 ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt
    30001 cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta
    30061 aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg
    30121 atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc
    30181 cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc
    30241 ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc
    30301 agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca
    30361 ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata
    30421 atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg
    30481 tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag
    30541 aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat
    30601 caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat
    30661 catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc
    30721 cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa
    30781 aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta
    30841 aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat
    30901 ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt
    30961 ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg
    31021 gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt
    31081 acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc
    31141 aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga
    31201 aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc
    31261 tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat
    31321 caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg
    31381 acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa
    31441 cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga
    31501 ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga
    31561 taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat
    31621 ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga
    31681 tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc
    31741 agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca
    31801 gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg
    31861 cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat
    31921 cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt
    31981 tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc
    32041 aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac
    32101 aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc
    32161 aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga
    32221 ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg
    32281 gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga
    32341 ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa
    32401 aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg
    32461 ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg
    32521 aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta
    32581 caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg
    32641 tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta
    32701 tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag
    32761 aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac
    32821 gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact
    32881 ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg
    32941 ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg
    33001 aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga
    33061 cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa
    33121 atatcattct aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca
    33181 tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag
    33241 cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt
    33301 cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc
    33361 ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc
    33421 gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta
    33481 aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa
    33541 attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta
    33601 acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt
    33661 gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa
    33721 ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa
    33781 attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta
    33841 gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga
    33901 atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag
    33961 gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg
    34021 tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca
    34081 tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca
    34141 acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc
    34201 gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga
    34261 ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac
    34321 acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc
    34381 gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga
    34441 actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac
    34501 acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga
    34561 agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga attttcattt
    34621 agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact
    34681 agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga
    34741 gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga
    34801 ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc
    34861 tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca
    34921 gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg
    34981 cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta
    35041 gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg
    35101 aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta
    35161 taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa
    35221 caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat
    35281 attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat
    35341 gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa
    35401 gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat
    35461 gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc
    35521 atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct
    35581 gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa
    35641 atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta
    35701 gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa
    35761 gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta
    35821 ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag
    35881 acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg
    35941 gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc
    36001 ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg
    36061 aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg
    36121 agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg
    36181 ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa
    36241 acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg
    36301 cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga
    36361 cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc
    36421 ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct
    36481 ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca
    36541 atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat
    36601 ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca
    36661 gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg
    36721 catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg
    36781 ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg
    36841 taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata
    36901 aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc
    36961 ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc
    37021 gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg
    37081 gacctccata tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag
    37141 acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg
    37201 atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa
    37261 tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc
    37321 aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat
    37381 tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct
    37441 atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg
    37501 aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca
    37561 aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg acaacaccat
    37621 ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg
    37681 aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa
    37741 tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga
    37801 ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt
    37861 tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg
    37921 ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag
    37981 aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc
    38041 tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg
    38101 attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg
    38161 ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg
    38221 gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc
    38281 gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac
    38341 cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg
    38401 aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg
    38461 aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag
    38521 ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag
    38581 accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag
    38641 caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga
    38701 gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata
    38761 tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata
    38821 tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag
    38881 cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg
    38941 tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt
    39001 acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc
    39061 tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt
    39121 atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat
    39181 tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg
    39241 agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga
    39301 tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag
    39361 caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac
    39421 aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt
    39481 caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat
    39541 ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc
    39601 aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa
    39661 aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg
    39721 gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt
    39781 ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt
    39841 agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt
    39901 gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc
    39961 caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct
    40021 tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt
    40081 tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga
    40141 atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat
    40201 gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca
    40261 gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata
    40321 gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg
    40381 cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag
    40441 ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct
    40501 gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc
    40561 tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg
    40621 cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg
    40681 ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt
    40741 tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt
    40801 tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa
    40861 cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca
    40921 aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca
    40981 tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg
    41041 gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca
    41101 cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt
    41161 ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg
    41221 tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt
    41281 tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct
    41341 taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg
    41401 cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca
    41461 acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact
    41521 ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat
    41581 gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag
    41641 tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt
    41701 caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc
    41761 cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa
    41821 gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt
    41881 tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc
    41941 gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac
    42001 aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt
    42061 acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat
    42121 atacattggg agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt
    42181 cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga
    42241 ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa
    42301 acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg
    42361 taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa
    42421 taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca
    42481 aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt
    42541 accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac
    42601 aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg
    42661 gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac
    42721 ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca
    42781 ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg
    42841 attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg
    42901 gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg
    42961 gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg
    43021 gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt
    43081 gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc
    43141 gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg
    43201 gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa
    43261 agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag
    43321 caggccattc catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt
    43381 aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa
    43441 gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa
    43501 gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact
    43561 tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt
    43621 agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt
    43681 tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt
    43741 tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca
    43801 ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac
    43861 caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg
    43921 atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa
    43981 gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta
    44041 cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg
    44101 tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa
    44161 gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa
    44221 aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca
    44281 gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt
    44341 tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa
    44401 tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt
    44461 ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta
    44521 tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac
    44581 taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag
    44641 ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat
    44701 ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa
    44761 tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac
    44821 atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc
    44881 aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag
    44941 cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa
    45001 ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac
    45061 cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc
    45121 tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag
    45181 gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag
    45241 aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt
    45301 tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt
    45361 atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg
    45421 tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg
    45481 aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag
    45541 ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat
    45601 ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag
    45661 gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg
    45721 ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga
    45781 aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa
    45841 tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg
    45901 accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc
    45961 ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat
    46021 acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg
    46081 agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc
    46141 agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa
    46201 tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc
    46261 tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat
    46321 ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta
    46381 ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac
    46441 gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac
    46501 cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat
    46561 caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc
    46621 cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt
    46681 ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa
    46741 agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg
    46801 tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga
    46861 tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa
    46921 agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa
    46981 agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa
    47041 agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa
    47101 acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag
    47161 tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg
    47221 gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga
    47281 agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa
    47341 tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg
    47401 cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg
    47461 gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca
    47521 atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg
    47581 atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat
    47641 cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag
    47701 cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt
    47761 caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac
    47821 tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa
    47881 ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc
    47941 cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa
    48001 tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg
    48061 tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag
    48121 cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca
    48181 gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc
    48241 cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat
    48301 tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga
    48361 acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct
    48421 tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg
    48481 tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt
    48541 ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa
    48601 aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa
    48661 tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag
    48721 atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac
    48781 gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca
    48841 tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc
    48901 taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct
    48961 agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc
    49021 gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga
    49081 agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg
    49141 aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat
    49201 tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata
    49261 aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt
    49321 ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta
    49381 tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat
    49441 ttagttgctt tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc
    49501 gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct
    49561 gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat
    49621 ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg
    49681 tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct
    49741 accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt
    49801 ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc
    49861 acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat
    49921 tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc
    49981 aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc
    50041 attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt
    50101 gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta
    50161 ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg
    50221 ttctttggta ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa
    50281 gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca
    50341 gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat
    50401 gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt
    50461 ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc
    50521 aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc
    50581 ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc
    50641 ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc
    50701 ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact
    50761 ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa
    50821 caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt
    50881 tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga
    50941 attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat
    51001 caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga
    51061 actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa
    51121 aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat
    51181 tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag
    51241 tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa
    51301 aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca
    51361 gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc
    51421 ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa
    51481 aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt
    51541 acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg
    51601 acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat
    51661 gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta
    51721 gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa
    51781 tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta
    51841 gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt
    51901 cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa
    51961 ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg
    52021 aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag
    52081 agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg
    52141 ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat
    52201 cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg
    52261 ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa
    52321 aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga
    52381 tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca
    52441 agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat
    52501 atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc
    52561 atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat
    52621 ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg
    52681 cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg
    52741 aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt
    52801 atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct
    52861 caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc
    52921 tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa
    52981 aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact
    53041 atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt
    53101 ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt
    53161 ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc
    53221 ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt
    53281 cattggaaag gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga
    53341 ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt
    53401 aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga
    53461 gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac
    53521 ccctgtatcc tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact
    53581 agttatcatt tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt
    53641 caagctctcg aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta
    53701 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat
    53761 gccttagtcc atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca
    53821 aatatttgct gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat
    53881 tatgagggtt tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt
    53941 ttaagagatc aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg
    54001 tttttcccca ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt
    54061 cgaaatcgat atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac
    54121 cagcaactaa cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt
    54181 tcattaaaga tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat
    54241 cttttccttt gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg
    54301 catctttcaa cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt
    54361 ctttagcata cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata
    54421 ccgctggcat atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag
    54481 gagatactgt tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata
    54541 gatttttgcg atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat
    54601 cttttgcttc tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat
    54661 cttttttgta tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa
    54721 ttgaatcagc tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt
    54781 ttattatttt ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt
    54841 aatggtataa ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt
    54901 gatttttaaa agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt
    54961 ctggtttttt attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa
    55021 gtaagagttg agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt
    55081 ttgccgacat cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat
    55141 aatctattta agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca
    55201 gtgaaatttt cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt
    55261 aattgattca gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga
    55321 agtaaacaat tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt
    55381 tctacctcgt catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg
    55441 aaaacatctt ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag
    55501 aatttctcga tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca
    55561 acgccaccaa tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct
    55621 tttttggtta gtttttcatt gctattatcc atagttacct cctattttgt catttccttt
    55681 acaagcattg tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct
    55741 aactcttagt ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca
    55801 ttgagcatag gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac
    55861 tttatcgccc tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga
    55921 aactttaggt gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat
    55981 aatggctatt ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc
    56041 actaatatat ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact
    56101 atctaagaaa gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag
    56161 gcttttattg ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt
    56221 gttattgtca tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat
    56281 cccgataata aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat
    56341 taacatgatt aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat
    56401 taatagatcc tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt
    56461 aaaatcgaat tcttcttgat taataatccc ttcatctaac aactctttat actttttaat
    56521 ttcatctgca cttgaaa
//
LOCUS       DAYYAG010000005        52156 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000005 DAYYAG010000000
VERSION     DAYYAG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.005"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1192)
                     /locus_tag="ACHARQ_000245"
     CDS             complement(<1..1192)
                     /locus_tag="ACHARQ_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8408704.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLN"
     gene            1506..1736
                     /locus_tag="ACHARQ_000246"
     CDS             1506..1736
                     /locus_tag="ACHARQ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8408705.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            1833..3134
                     /gene="murA"
                     /locus_tag="ACHARQ_000247"
     CDS             1833..3134
                     /gene="murA"
                     /locus_tag="ACHARQ_000247"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8408706.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            3147..3323
                     /locus_tag="ACHARQ_000248"
     CDS             3147..3323
                     /locus_tag="ACHARQ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8408707.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            3458..3754
                     /gene="yidD"
                     /locus_tag="ACHARQ_000249"
     CDS             3458..3754
                     /gene="yidD"
                     /locus_tag="ACHARQ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8408708.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            3939..4406
                     /locus_tag="ACHARQ_000250"
     CDS             3939..4406
                     /locus_tag="ACHARQ_000250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8408709.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            4491..5375
                     /locus_tag="ACHARQ_000251"
     CDS             4491..5375
                     /locus_tag="ACHARQ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8408710.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            5489..5725
                     /locus_tag="ACHARQ_000252"
     CDS             5489..5725
                     /locus_tag="ACHARQ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8408711.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            5813..5893
                     /locus_tag="ACHARQ_000253"
     tRNA            5813..5893
                     /locus_tag="ACHARQ_000253"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5847..5849,aa:Tyr,seq:gta)
     gene            5900..5971
                     /locus_tag="ACHARQ_000254"
     tRNA            5900..5971
                     /locus_tag="ACHARQ_000254"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5932..5934,aa:Gln,seq:ttg)
     gene            6096..6266
                     /locus_tag="ACHARQ_000255"
     CDS             6096..6266
                     /locus_tag="ACHARQ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408712.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            6486..7193
                     /locus_tag="ACHARQ_000256"
     CDS             6486..7193
                     /locus_tag="ACHARQ_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8408713.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(7252..7896)
                     /locus_tag="ACHARQ_000257"
     CDS             complement(7252..7896)
                     /locus_tag="ACHARQ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8408714.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            8022..8309
                     /locus_tag="ACHARQ_000258"
     CDS             8022..8309
                     /locus_tag="ACHARQ_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408715.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            8417..8737
                     /locus_tag="ACHARQ_000259"
     CDS             8417..8737
                     /locus_tag="ACHARQ_000259"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8408716.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            8818..10131
                     /locus_tag="ACHARQ_000260"
     CDS             8818..10131
                     /locus_tag="ACHARQ_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8408717.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            10128..10847
                     /locus_tag="ACHARQ_000261"
     CDS             10128..10847
                     /locus_tag="ACHARQ_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8408718.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            10975..11526
                     /gene="raiA"
                     /locus_tag="ACHARQ_000262"
     CDS             10975..11526
                     /gene="raiA"
                     /locus_tag="ACHARQ_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8408719.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            11748..14282
                     /gene="secA"
                     /locus_tag="ACHARQ_000263"
     CDS             11748..14282
                     /gene="secA"
                     /locus_tag="ACHARQ_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8408720.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            14408..15509
                     /gene="prfB"
                     /locus_tag="ACHARQ_000264"
     CDS             join(14408..14479,14481..15509)
                     /gene="prfB"
                     /locus_tag="ACHARQ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8408721.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            15614..16300
                     /gene="ftsE"
                     /locus_tag="ACHARQ_000265"
     CDS             15614..16300
                     /gene="ftsE"
                     /locus_tag="ACHARQ_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8408722.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            16293..17177
                     /gene="ftsX"
                     /locus_tag="ACHARQ_000266"
     CDS             16293..17177
                     /gene="ftsX"
                     /locus_tag="ACHARQ_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8408723.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            17329..18777
                     /gene="cls"
                     /locus_tag="ACHARQ_000267"
     CDS             17329..18777
                     /gene="cls"
                     /locus_tag="ACHARQ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8408724.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            19114..19971
                     /locus_tag="ACHARQ_000268"
     CDS             19114..19971
                     /locus_tag="ACHARQ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8408725.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            20086..21006
                     /gene="pstC"
                     /locus_tag="ACHARQ_000269"
     CDS             20086..21006
                     /gene="pstC"
                     /locus_tag="ACHARQ_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8408726.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            21006..21890
                     /gene="pstA"
                     /locus_tag="ACHARQ_000270"
     CDS             21006..21890
                     /gene="pstA"
                     /locus_tag="ACHARQ_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8408727.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            21905..22711
                     /gene="pstB"
                     /locus_tag="ACHARQ_000271"
     CDS             21905..22711
                     /gene="pstB"
                     /locus_tag="ACHARQ_000271"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8408728.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            22727..23485
                     /gene="pstB"
                     /locus_tag="ACHARQ_000272"
     CDS             22727..23485
                     /gene="pstB"
                     /locus_tag="ACHARQ_000272"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8408729.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            23498..24175
                     /gene="phoU"
                     /locus_tag="ACHARQ_000273"
     CDS             23498..24175
                     /gene="phoU"
                     /locus_tag="ACHARQ_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8408730.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            24752..25444
                     /locus_tag="ACHARQ_000274"
     CDS             24752..25444
                     /locus_tag="ACHARQ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8408731.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            25441..26208
                     /locus_tag="ACHARQ_000275"
     CDS             25441..26208
                     /locus_tag="ACHARQ_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8408732.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            26308..27876
                     /gene="liaX"
                     /locus_tag="ACHARQ_000276"
     CDS             26308..27876
                     /gene="liaX"
                     /locus_tag="ACHARQ_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8408733.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            28173..28493
                     /locus_tag="ACHARQ_000277"
     CDS             28173..28493
                     /locus_tag="ACHARQ_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8408734.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            28493..28843
                     /locus_tag="ACHARQ_000278"
     CDS             28493..28843
                     /locus_tag="ACHARQ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8408735.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            29010..29948
                     /gene="hprK"
                     /locus_tag="ACHARQ_000279"
     CDS             29010..29948
                     /gene="hprK"
                     /locus_tag="ACHARQ_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8408736.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            29963..30793
                     /gene="lgt"
                     /locus_tag="ACHARQ_000280"
     CDS             29963..30793
                     /gene="lgt"
                     /locus_tag="ACHARQ_000280"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8408737.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            30837..31862
                     /locus_tag="ACHARQ_000281"
     CDS             30837..31862
                     /locus_tag="ACHARQ_000281"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8408738.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            31877..32815
                     /gene="galU"
                     /locus_tag="ACHARQ_000282"
     CDS             31877..32815
                     /gene="galU"
                     /locus_tag="ACHARQ_000282"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8408739.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            32940..33380
                     /locus_tag="ACHARQ_000283"
     CDS             32940..33380
                     /locus_tag="ACHARQ_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8408740.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            33383..33985
                     /locus_tag="ACHARQ_000284"
     CDS             33383..33985
                     /locus_tag="ACHARQ_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8408741.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(34044..35150)
                     /locus_tag="ACHARQ_000285"
     CDS             complement(34044..35150)
                     /locus_tag="ACHARQ_000285"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8408742.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            35360..36361
                     /gene="ccpA"
                     /locus_tag="ACHARQ_000286"
     CDS             35360..36361
                     /gene="ccpA"
                     /locus_tag="ACHARQ_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8408743.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(36698..39043)
                     /locus_tag="ACHARQ_000287"
     CDS             complement(36698..39043)
                     /locus_tag="ACHARQ_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8408744.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            39322..40224
                     /locus_tag="ACHARQ_000288"
     CDS             39322..40224
                     /locus_tag="ACHARQ_000288"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8408745.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            40437..42212
                     /locus_tag="ACHARQ_000289"
     CDS             40437..42212
                     /locus_tag="ACHARQ_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8408746.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(42278..43864)
                     /locus_tag="ACHARQ_000290"
     CDS             complement(42278..43864)
                     /locus_tag="ACHARQ_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8408747.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            44115..44339
                     /locus_tag="ACHARQ_000291"
     CDS             44115..44339
                     /locus_tag="ACHARQ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8408748.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            44480..46231
                     /locus_tag="ACHARQ_000292"
     CDS             44480..46231
                     /locus_tag="ACHARQ_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8408749.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            46231..48015
                     /locus_tag="ACHARQ_000293"
     CDS             46231..48015
                     /locus_tag="ACHARQ_000293"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8408750.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            48341..49192
                     /gene="licT"
                     /locus_tag="ACHARQ_000294"
     CDS             48341..49192
                     /gene="licT"
                     /locus_tag="ACHARQ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8408751.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            49288..51168
                     /locus_tag="ACHARQ_000295"
     CDS             49288..51168
                     /locus_tag="ACHARQ_000295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8408752.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(51208..52110)
                     /locus_tag="ACHARQ_000296"
     CDS             complement(51208..52110)
                     /locus_tag="ACHARQ_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8408753.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        16838 a         9117 c        11124 g        15077 t
ORIGIN      
        1 tgtttaagca ttccaatggt ccgtttgaat agctgtagtg aagcgcattt tggatctgtg
       61 gcagaaactt tttaaacgtt cgtaaagtgg ttgtataggt ttctggcaga ccgctaatgt
      121 ctctagttaa acaatcctca aatagtgact gatctctttt ctttaccgca taaagaatgt
      181 cttggtataa ctgatagcct tggcgtaagt tgggacaagc agctaacagt cgatcggcaa
      241 tctcactttc agtcaaataa tctttaaaac tggcacgcca aacccgtttt tcatagttta
      301 actttgcttc ttctttttgt aggagtttcc aatagcgctt caattttttc cctaaccctc
      361 gttgagagtg ggaatttcct ttaagtcgta ccgtggtttc tttcactcga tgattcaaga
      421 aagttctgcc aatatgttgg acgatgtgga aacgatcgat gatcaactga gcattcggga
      481 aaagcttttt caccaacgaa acataaggcg catacatatc aataacaatc aattgaacgg
      541 cttcacgagt cgctaatgaa aaccgagaaa aatagcgttc taaataaggc aagcgtcgat
      601 tttcgacaat atccaataat gcatggcttt gtccgtccat catgataaaa ctcattgcac
      661 tggcaacact tcgaaccgat ttgaattcat caaagcataa gacctttggc aaagtctgtt
      721 tgaaaggatt caatgggcgg tagaatcgct tcatgacacg atagaccgaa gaagtggaaa
      781 tatttttctg acgagcgatt tccgtcatgg aagtattctc ttttaagcga gcaatgacag
      841 accaacgaac tttttgacta atgaaacagt gtttttcagc aacagaggta tccgctacga
      901 atgttctttg gcaagactta catttaaatc gttgtttctt cagctccaga taagtgcgat
      961 actcagacac atcattcagt aacaaccgaa cggttgtcgt cccccaacga atgatcctag
     1021 aggggacgac acaaagacaa tgggaacact cttctggctt gtacagaaga gttcctttgt
     1081 aaatcaagca aatttgccct ttgtattttt ctttagttaa acaattttcc tcgaaataga
     1141 tatttaggtc tagaatattt agggtatctt tgataagctg agtatgagac atcttgaatc
     1201 ctcctatgaa ttggtagcca aattaattct aaaggatttc agggtgtctt ttctatattt
     1261 ttttatacaa aaaagatgtt ggtgaagttc accaacatca aaaagtatag agcctttatt
     1321 tggaataagg gactgcgaca agacttttgt ctcagtccct ttttttgcat atttttagca
     1381 tttatttcct aaaaaatgta aggggattcc tatattttta agtatacttg tttttccttt
     1441 tatacatttt atggtattat cattttatta gttattataa aacaatcgtt agaaaggacg
     1501 tcattatgca agtatacgga attgatgcgg tgcttcgaat cgtgagtcat ttggcgttta
     1561 tctacttagc cttctggtct ttacgttcgt tacgcattga aacttttttc agaccaatgc
     1621 ataatacgca aattagaatg gcaatcgtac ttttttcaat ttttttaggg tacactgcaa
     1681 gtaatttttt cttagaagtt atcgctttat gccgaaatct tttcgttagc ctgtaaattt
     1741 taaaaaaagg tactatttta aaagcaagga tgttataata gaaaagattt tgagtaagtg
     1801 tatgctcatt ggaatttttt ggagggaaca acatggaaga gatcatcgta agaggtggca
     1861 atcaattaaa tggaacagta agaattgaag gagctaaaaa tgctgttttg cctattttag
     1921 ctgctagttt gttagcagaa gaaggtatta cgacattaga caacgttccg attctttctg
     1981 atgtcttcac tatgaatcaa gtgattcgtc acttaaacgt agatgtagat tttgatgaac
     2041 aaaagaatca agtaacaatc gacgcttcaa gacaattaga aatcgaagcg ccttatgagt
     2101 atgttagtca aatgcgtgct tcaatcgttg tgatgggtcc gctacttgcc agaaatggac
     2161 atgcaaaagt agctatgcct ggtggatgtg cgattggtaa acgaccaatc gatttgcatt
     2221 taaaaggatt tcaagcttta ggagcaaaaa ttattcaaaa aaacggctat atcgaagcaa
     2281 ttgcggatga attgatcggt aacacgattt atttagattt ccctagtgta ggtgctactc
     2341 aaaatatcat gatggctgcc gtaaaagcaa aaggaacaac catcattgaa aacgtagctc
     2401 gcgaaccaga aatcgttgat ttagcaaaca ttttgaataa aatgggcgct caagtctatg
     2461 gagctggtac tgaaacaatg cgtattgaag gcgtggatca tttacatgca gtcaaccact
     2521 caatcgtaca agatcgtatc gaagcaggta cctttatggt tgctgctgca atgacacaag
     2581 gcaatgtgct gattgcagat gctatctctg aacataaccg cccattgatt tctaaattga
     2641 tcgaaatggg tgcagaaatc attgaagaag aaggcggagt ccgtgtcatc ggaccaaaac
     2701 atatcttacc tacagatgta aaaacgatgc ctcatcctgg tttccctaca gatatgcaag
     2761 ctcaaatgac agctatccaa ttagttgcag aaggtacaag tgtagtgact gaaacagtgt
     2821 ttgaaaatcg tttccaacat ttagaagaga tgcgccgtat gaacgctcat gtcaaaatcg
     2881 acggaaatgt agcaatcatg gatggaaacc atgaattgca aggtgcagaa gtatatgcaa
     2941 cagacttgcg ggcagcagca gcattagttt tagctggctt gaaagcaaat ggaatcacac
     3001 gtgtgcgtaa cttgaattat ctagaccgtg gatattataa tttccatatc aaactacagc
     3061 aattaggtgc agacgttgaa cgtgtagaca tggatcaaac ttctgctgaa aaaacagcac
     3121 aaacgattgc ttaaggaagt ggctgaatga gtaaccaacg atatatctta ttaactttga
     3181 taaagatttt aatggtgatc ttattgctta tccttctttt tgtggccgga acgatgatcg
     3241 gatatggtgt catcggtggc ggcaatccat ttaaagtatt tcagccaagt ttgtggattc
     3301 atattcgaga cttttttcat tgataaataa gaatcacagt ggttctagtc gatatgaagt
     3361 gagccatgaa gcagaacatg ataaaagaaa acagaaggga acgttgacgt tagtcgcgat
     3421 cccttttttt agtaaaagaa aagaaggaat ctaattgatg aagaagatat tgatttattt
     3481 agttagagga tatcagcgct ttatttcgcc tgcatttcca cctagctgcc gttattaccc
     3541 aacctgttcc aattatatga taaaagcaat tcaaacccat ggcgcgctaa aagggacggt
     3601 aatggggact gcacggatct tacgctgtca tccttttgtc aaaggcggta tcgattatgt
     3661 accattgaca ttccgattaa cgcgaaataa gaaagataaa aaagaaccag attataaaaa
     3721 ggttgtgaaa aatacgggta gcactgagcg ataaagaaac aatttggaaa atagcttctt
     3781 atattttgca aaattgtgag ttattgccga agtgttggat tttgaacacc gtcaaaaaaa
     3841 aggttgtgaa aaatacggct agcactgagc gataaagaaa aagaggaaat ttattgtcaa
     3901 agtaacaact ccgcaactac ttatatgggg gacaaaaaat gatcgaagaa ctaaacaaga
     3961 aagatttacc atggtccttg ttattagatg cagatcctga taaagaaaaa gttcaagtat
     4021 atgtttccag agggagtggc tggatatgga aagaaaaaga aaaaacaata ggtgtactga
     4081 tctatgtggc aagggaaaaa gaatacgaaa tcgtcaatgt agcagtaacc ccaacgtatc
     4141 aaggaaaggg gattggcgga aaattacttg agacagcttt tcagaagctt tcacaactcg
     4201 cttccagtca aactcagatc atcatacgaa ccggcagtac tagcagtgcg gcgttacatc
     4261 tctatcaaaa aatgggctat gtggagattg gtcgagtcaa agactatttt atccatcatt
     4321 actctgagcc catttttgaa aatggagatc aattaaggga tcaagtgatt ttagcaattc
     4381 acttacctaa aaatagcttt aactaataaa atttcaattc aagaaaatgc tttcaaatgc
     4441 taaatagata cattacaata gctttagcaa acaaaaagga gaatattttt atgggaatat
     4501 tagtttttga ttttggtgga tcggctgtca aatatggttg ttgggatggc aaagagatta
     4561 aaggtaaggg acaatttgca acgcctgaat cttgggaaga aatgaaagcg caattatttc
     4621 aagtttataa aaaaatactc gtttcttttg aagtcgaagg aattggaatc agctctcctg
     4681 gagtagtgaa taatgagaaa caggtgattg agggaatcag tgcgattcct tatattcatg
     4741 gatttaatat tttccgagat ttggaaactc tgtttcaact tccagtaacg attgaaaatg
     4801 acgccaactg tgccggaatg gctgaatttt atgaaggtgc tgcccgcgat tatcaagatg
     4861 ttgcttttgt tgtggcagga actgggattg gcggggcgtt gttcacgaaa ggacaaatca
     4921 ataaaggggc acatctttac ggtggagaat ttgggttgat gttcttaaat ggcgataaga
     4981 cattcagtaa acttggtaca gcggtacaga tggcttggcg ttattgtgaa cgcaaaggtc
     5041 tcgataaaaa tgcttatacc ggaaaagatg tctttgaatt agcagaaact ggagatgcaa
     5101 tagcaaaaga agaagttgag agtttttata cgtatcttac aaagggatta ttcagtattc
     5161 aattttcgtt tgatccagaa gtgatcgtat taggcggcgg ggtttctgca aaagaagggc
     5221 tcattgatga gatcaaaagc cgaatgaaga agctgacaga acaattcgat ctgcatgatt
     5281 tcgaaccgca gattttatta tgtgaataca gaaacgatgc taatcttgta ggtgcagcag
     5341 caaactatct cgctgtacgt caaggggaga attaagagct ttagagaagg tgcagcttgc
     5401 ataaaagaag tgaatgaaat gaataggaga tttttaccta aggaatgttt tatttgctgc
     5461 agtatgataa aatatctgag aggtgaaaat gatgtcaaaa aaaaataaag atattgaagt
     5521 gcgtatagaa gaagtgaaaa aagatatcaa aggtaagaat tatgaagtca ctcagttatt
     5581 tatagggaaa aaaatgattg gtgagatctt agcctatgga ccaaaagaat atgaaatttt
     5641 ctttggtgaa gaagattttg gaaaagaaaa aagtcttgaa aatgcgatag aaacagtgat
     5701 tcgacactgg aatctgcacg aataaaaaaa tatcaaaaaa actttcaata aagggttgca
     5761 aaaatctgga aaattaggta ttataactaa gtcaggttga tacaaacctt tcggaggagt
     5821 agcgaagtgg ctaaacgcga cggactgtaa atccgttcct tagggttcag tggttcgaat
     5881 ccactctcct ccatttcatt ggggtatagc caagcggtaa ggcaagggac tttgactccc
     5941 tcatgcgttg gttcgaatcc agctacccca gtataccaca atcgtaagct ctcgttagtt
     6001 cgagagctct tttttttgct ttgctacctt gttccttgaa gattctgctg atttaagata
     6061 gagttaagtt aactacaaga attggaggga tccacatgaa atggaaagaa aaagtagacg
     6121 aagcagctga aaagttatat gatttggtca aatcagaaaa atacaatatt gaagtgaata
     6181 ttccgaaaaa aggcggaaaa gcggtccggg taaaatcaaa acgcccaacg aatcatacaa
     6241 aaaaatggat ggctaaaaac cgctaataat aacaaaagat ccataagtcg gcattttcat
     6301 cgattttagg gtcttttgtt attttctagg ttggacatat ctaaaaaatt gtttctgaca
     6361 ataggagtag gaagaacgat gtgtgtctaa atcctctgtt gatggataga aacctttatc
     6421 tttttcagtt gtgaatcagg tatataccag ttataataga agaaacgaaa ggacgggtgt
     6481 acgttatgtc gaatcaatta cctgtatata tacagattca tgaccaatta aaaaatgaaa
     6541 tcgaacaagg tatatggaaa gtcggagacc gtcttccttc agaaagagaa ttagcagtga
     6601 aattcaatgt cagccgtatg acgttgagac aagcaattca aaacttggca gatgaaggaa
     6661 tcttagaaag aaagatcggt tcgggtacat atgttgcacg agaaaaagta caagaaaaaa
     6721 tgtctggtgc aacaagtttt acagaaatta tggaatcaca aaatcgagta ccgtctagtc
     6781 ggacaatctc ttatttcttg acttcgccaa gctcaagtga aatggaaaaa ctaaacttga
     6841 gtaaagatga cacgatcttg cgaatggaac gtattcgttt cgcagatgat gtccctattt
     6901 gttttgaagt tgccagtgtg ccacaaaagc tgatcgatgg ctacagcaaa gcagagatta
     6961 ctgaatcgtt gtatcgcacg ctagaagaaa aaggcgggaa aaagatcggt ccagcgaatc
     7021 aaacgattac tgctgtgttg gcttctgaga aaattgctga ctatttagag gtaaaacgag
     7081 gagatgcagt tcttcgtttg cgccagattt cttattttga agacggtacg ccgtttgagt
     7141 atgtgcgtac gcaatacgta ggaaatcgtt ttgagtttta tttggaaaaa taaaaaacag
     7201 gtgatccctg attccgtatt agaaataaaa gagaatcaga atcacctgtt tttatatatg
     7261 atcagttacc agttgctcgt gataagaaac aggaccctct tcaaattgga cttgtccgtt
     7321 cagcagctca atgatggatt ctttaaagtg gtcagcactt ttttcatcta cagcacagat
     7381 gacgctaact tgatcagtat attggatttc ttttataaaa aaatgttgga gttcaagata
     7441 attctgtaat tttcctagtt gcggatacgt gatcatcaca gagatctcct gttgcaaggt
     7501 accttctacg attccaatgt cagacaatgc ttgagagacc gcatgagtgt atgctcgtat
     7561 taatccgcct gtgccgagtt tcgttcctcc aaaatatcga gtgactactg cagctacatt
     7621 gatcagttga ttttttttga ggacttccaa catcggtatg cccgccgttc cacttggttc
     7681 tccatcatcg ctgcttcgct ggatttcatt tttttctcca atgatgaagg cgctgcagtt
     7741 atggttcgct ttccaatgtt ctttttttat ttcttgaatg aaactttttg cttcgtcctc
     7801 tgattctatt cgtctaagag aacagataaa gcgtgatttt ttgatctcga tctcactttg
     7861 tccattttct tttattgtac gatagcgctc gatcatcttg tcacctcttt tttcattttc
     7921 tttattcagt ataaataaag ttctaggttg tttcaacact gtaacggttg cgcaattgct
     7981 gtagtatgcg ataataaaga caaaggcagg tggatctggg gatgcaggaa cgaatgaaaa
     8041 agtacgatgc aattacgcat tatttgaaga acaatggcgg ctctcaagta acgttgacct
     8101 ttacccaatt tgacgaactg ctgtttccca gtaacggact tcccaaaaca gcaagggaaa
     8161 gtacagattg gtgggcaaat gattataaac accctgaaaa aggtgcctat ggctggataa
     8221 atgcaggcta tgaagtagtt gtcatcaatt tggacaaaga atatgtcgtg ttcaataaat
     8281 tagtgaaatc tagttggcta tttgattaaa taaaccgcgt ttagaaaacg ttgacaaatt
     8341 tctttctttt tatataatag agtaacagac gcgaagaaaa cgtttgcgta gtttgtttta
     8401 aaaaggagag aaaagtatgg ctaaaaaaac aatcatgctt gtatgttcgg ctggtatgag
     8461 cacaagttta ctagtaacaa aaatgcaaaa agctgctgaa gcaaaaggaa tcgactctga
     8521 tattttcgct gtgtccgctt ctgacgcaga taacaatctt gcaaataaag acgtggatgt
     8581 gttgttatta ggcccacaag ttcgctttat gaaagctgat tttgagaaac gtctggaacc
     8641 aaaaggcatt cctttagatg tcatcaatat ggctgattat ggtatgatga atggcgaaaa
     8701 agtgttagat caagcaatca cgctcatagg tgaataaata gaacagtata actatcgaac
     8761 aagcgatcca aaaaatagat cgcttgtttt ttcgtatatt tttttagggc gaataatatg
     8821 aatcattttt ggggtagaag gcttctgacg agagatatta aagcaataga ctgtgaggag
     8881 gtaaatttac caaattatga gaagatcgca gcagtggaac gtttgagcaa ctggattctt
     8941 tgtcatcgct gtggaatgag aacgtccgtc attgatggac agcttgcaga ttacggttat
     9001 ttttgtacac attgtctata tttagggcga tgcgacactc aacaagattt atatttgttt
     9061 gatcagcctg aagcaaaacc tcgagaagtc gtcttttttt ggacaggaca gcttactgaa
     9121 aaacaaacgg aaatctcaaa gagaatactt tgccattcag aaaaaagaca tcatcttatt
     9181 tgggctgtaa caggtgcagg gaaaacagag atgctttatc ctattttagt ggaaacttta
     9241 aaagctggag gaagagtagc gatttgtaca ccgcgagtcg atgtttgtaa tgaattgttt
     9301 ttacgctatc gttatgtttt tccagaagaa aaaatcacgt tgctccatgg gaatacagca
     9361 acggcttata cctttacttc ttttgttatc tgtacgattc atcagctata ccgtttttat
     9421 cggtcctttg atttaatagt gatcgatgag atcgatgcgt ttccttataa cggggatgca
     9481 ggattagctc atgctgtgca aacagcgatt aaaccagacg gacgattcat ctacttaagt
     9541 gccacacctg ataaaaagtt actaaaggag atcaagcaga cgtttgacat ccacaaactt
     9601 gctttacgct ttcatcggcg tttgcttcct gaacccgtct tggttttcat gaataattgg
     9661 cgacaaaaat gcaagaacaa gaagaagata aaatctttgg tgcggatcat tcatcaatta
     9721 attaaagaaa accatatcct tttattttgt ccaagtatca cgatgatgca tcgtttgaaa
     9781 gccacacttc aagaggctct tccgagatat gagatcacag atgtttcttc aagagacaat
     9841 cagcgggcgg aaaaagtcca gaaaatgcga gaagcaaaat atgacatact gttaaccacg
     9901 atgatccttg aacgtggagt cacatttgaa agaatatccg ttatcgtttt aggagcggat
     9961 catccggttt ttactaaatc ttctttggta caaatcgcag gaagagccga tcgtaaaggg
    10021 ccttatacga acagtcaagt atattttttc tatgaagaaa aaactgcagc tattcgaaaa
    10081 gcttgtcagg aaatcaaaga aatgaatgaa gaagggaaga agatactgtg aggtgcagtt
    10141 gctgcagtaa agatatcgtc caaaatttaa cattcagcga gattattggt gtttcgaaat
    10201 acagattacc tcagacttgt tcagtttgtc gcgcacaatt tcatttattg gaaacaagag
    10261 gctgttctgg ctgtcaaaaa ccaatccatt tcaatctaga gagtacgaaa aagaaagtaa
    10321 attctatccc cgtttactgc agtgactgtc aagaatggaa gaagaaatat ccgacctatg
    10381 attttcagca ccgtgccttt tttgtgtacg atgaaaatat gaaggcctgg ctcaatcaat
    10441 acaagtttat gggagatatc cgactagcag gcacctttgc atataaatgg acgcagataa
    10501 aaaaagaata cccgacatac atctattgtc caataccttt atctgaaaaa aggcgaacag
    10561 aaagaggatt caatcaagta agcgagatgt tgaaagcagc aagaattcct tttcagatgc
    10621 tactcaaaag agagaagcat ctgctggctc aagcaaaaaa cacgcgaaag gaaagattgg
    10681 agatgcctca accttttgaa cttgcagtca ccttttctaa gatacaaaat aaaaaaatat
    10741 tgattattga tgatgtctat acaacaggac agactctttt ccacgcagca gattgtttgc
    10801 agatcgcaca gccgaaaatc atccgaacta tttctttggc gagatgaatt agatgtggaa
    10861 atttgatagc gatttaattt gcaaataacc tagagttcga tataatagaa ttaagaagag
    10921 ggaaagagtg gtccttctcc ttcttgattg tggtagacga aaggggtaat ttttatgttt
    10981 agatataatg tacgcggcga aaatatcgaa gtaactgaag cgattcgcga ctacgttgag
    11041 aaaaaagtag gcaaactaga acgctatttc agtgacgcac ccgatgcaac ggcgcatgtg
    11101 aacttaaaag tttatacaga aaaaacagca aaagtggaag taacgatccc attaccttat
    11161 cttgtattac gtgccgaaga aacttctcca gatttatacg caagtatcga tttggttgtt
    11221 gacaaattgg aacgccaaat ccgcaaattc aaaacaaaaa ttaatcgtaa atcccgtgaa
    11281 acgggcatga atactgcaaa tgctgctgct ttattcaatg aagaggacga aaacgaaaat
    11341 gattctgaat tagatatcgt acgtacgaaa cgcctttcat tgaaaccaat ggatagtgaa
    11401 gaagcagtct tgcaaatgaa catgctggga cataacttct ttatttttga agatgcagaa
    11461 acaaatggta caagtatcgt ttaccgccgt aaagatggaa aatacggact gattgaaaca
    11521 gactgatcag ccttacttaa ataacaacga aaacttgcag cagctactga cagctgccgc
    11581 aagttttttt gagtttcctc ctcttttaga tgaaatatga ataaattatg aagttcttac
    11641 tttaatacgc ttaagatatt tcaattatct ataactaatg ataaaatgaa aagtaaagtc
    11701 tacatagtct ggataataca cagaaggaaa aggagattac acaacgcatg gccaatttga
    11761 ttcgttcact tatcgaaaac gataagaaag aactaaaacg tttagatagc attgcaaaaa
    11821 aagttgaagc atacgctgac caaatggcag cattaactga tgaacaatta caggcaaaaa
    11881 ctgctgaatt caagggcaga taccaaaaag gagaaacatt agatcaatta cttccggaag
    11941 cttttgcagt cgtccgagaa gcagcaaaac gtgtattagg tttatatcca taccacgtac
    12001 aattgatggg tggaatcgtg cttcacgatg gaaatatccc tgaaatgcgt actggtgaag
    12061 gtaaaacctt gaccgcaacg atgccagtat atctaaatgc tctgactggc gaaggggtac
    12121 acgtagttac cgtcaatgaa tatttagcaa cccgtgactc gacggaaatg ggcgaacttt
    12181 ataatttctt aggattaaca gtggggttga atatcaactc aaaatcttcg gatgaaaaac
    12241 gcgaggctta taattgtgat atcacttata gtacgaataa tgaattagga tttgactact
    12301 tgagagacaa tatggtcgtt taccgccatc aaatggtaca acgtccgctg aattatgcca
    12361 ttgtcgatga agttgactca atcttgattg atgaagcgag aacgccgctg atcatttcag
    12421 ggcaagcaga aaaatccaca gctttatata caagagcaga caacttcgtg aaacgtttga
    12481 aagaagaaga agattataaa atcgatgtac aatcaaaaac aatcagttta accgaagcag
    12541 ggatcgaaaa agcagaagaa acattcggtt tggataactt atacgacatt gaaaatactg
    12601 cattgaccca tcacttggat caagcattga gagccaacta tatcatgctt ttagatatcg
    12661 attatgttgt ccaagacgga aaagtactga ttgttgacca gttcactggt cggatcatgg
    12721 atggacgccg ttattcagat ggacttcacc aagcaatcga agctaaagaa ggcgtagaga
    12781 tcgaagacga aacgaaaacc atggcgacga tcactttcca gaactatttc cgtatgtaca
    12841 agaaacttgc gggaatgact ggtacagcta aaacagaaga agaagaattc agagaaattt
    12901 ataacatcca agtataccag attccgacaa atcgtccggt tatccgtgat gatcgtccag
    12961 atttgcttta cccaacatta caaagcaagt tccacgctgt cgtgcaagat atcaaagatc
    13021 gctaccgcaa aggtcaacct gtgttagtcg gaactgttgc ggttgaaaca tctgagttat
    13081 tatcagatat gctgaatcaa gaacgtattc ctcatgaagt gctgaatgcg aaaaatcact
    13141 ttaaagaagc agagatcatc atgaatgctg gtcaaaaagg tgctgtgacg attgctacca
    13201 acatggctgg tcggggaaca gatatcaagt taggacttgg ggtaagagaa cttggtggtc
    13261 tagcagttat cgggacagaa cgacatgaat ctcgtcgtat cgataaccag cttcgaggac
    13321 gtgcaggacg tcaaggagac cctggtatgt cacagttcta cttgtcattg gaagatgatt
    13381 tgatgaaacg attcggttca gaacgaatca aatctttctt agatcggatg aagatcggtg
    13441 aggaagatgc tgttatccaa agcaagatgc tgacgaaaca agtagaatct gcacaaaaac
    13501 gtgtagaagg aaataactat gacacacgta aaaacgtctt acaatacgat gatgtgatgc
    13561 gtgaacaacg tgaagtaatc tataaacagc gacaagaagt aatcatggaa gaaaaaagct
    13621 tatccaagcc actaatgaac atggtcaaac ggacaatcag tcgtgtagta gatgcgcata
    13681 cacaattaga gaaagaaaat tggaatttgg aaagtatcat cgattttgcc ggcagtactc
    13741 ttgtccatga agatagtatt actcttgcag atatcgaagg aaaaacagct caggagatca
    13801 aagagtatct aaacgaacga gcacaagaag tatttaatac caaagcggct caactaaatg
    13861 gtccagagca actgttggaa tttgaaaaag tcgtgatctt gcgtgttgtg gactctaaat
    13921 ggacagacca tattgatgca atggatcaat tacgtcagtc aatcggctta cgtgcttatg
    13981 gacagaacaa tccattagtc gaatatcaaa cagaaggcta taagatgttt gaagatatgg
    14041 taggcgccat cgagtatgaa gtcactcgaa tctttatgaa agctgagatc cgtcaaaacg
    14101 tacagcgtga acaggtttct caaggaacag cttctcactc agaagacgga gatgccaaag
    14161 aaaatagtaa tactagcgcg aaaaaacagc caatccatgt gaacaaggtc ggaagaaatg
    14221 atccttgtcc ttgtggaagc gggaaaaaat ataagaactg tcatggaaaa aatgcaaact
    14281 aatttctgac agcaggattg gtgagacaaa ttcttttttt aagaattcgt ctcaccttct
    14341 ttctgttatt tgctgattct gttagactag atataatgaa aaaagtatag tgaaagaagg
    14401 aaaaaacatg gaaatatccg aaattcgaaa tctcctgaca gaaatgcagg aaaaggtcac
    14461 aagcttcagg aggtctcttt gacttagacc agctggaaga agacattgcg gaagcagaag
    14521 caaaaatggc tgaacctggt ttttgggatg atagcgaagc tgctcaaaaa gtgatcagtg
    14581 aaaacaatgc aaataaagaa acttatgatc aattccattc tcttgctgaa gaactggaag
    14641 aaatcgaatt gatggtggag atgttgcagg aagaacctga tgcagccatg caagaagaag
    14701 cagaaacacg tatcaatgcg ctagatgaaa aaatgaagac atatgaatta tctatgttgt
    14761 tagatggtcc ttatgatcga aacaacgcca tcgttgaatt gcatccaggg gctggcggaa
    14821 cggagtccca agattggggc agtatgcttt tacgtatgta tacacgctgg gcagagcagc
    14881 atgggtatca agtagagaca ctagattatc aagcaggtga tgaagcagga atcaagagtg
    14941 tgaccctact gatcaaagga cacaacgcat ttggttatct gaaatcagaa aaaggtgttc
    15001 atcgccttgt acgtatctcg ccttttgact cagcgaaacg acgtcatact tctttttgtt
    15061 cggtagatgt gatgccagag cttgatgaaa cgatcgatat agaaatcaat ccagatgatt
    15121 tgaaaatcga tacgtaccga gcaagtggtg caggtggtca gcacatcaat aaaacagaat
    15181 ctgctgtacg gatcactcac ttgcctacag gaacagtagt agctagtcag gcacaacgtt
    15241 cacaattgaa aaaccgtgag caagcgatgg ggatgttaaa agctaagctt tatcaattag
    15301 aagtggaaaa gaaagaacaa gaagcagctg cacttcgagg agaacaaaaa gaaatcggct
    15361 ggggttcaca gatccgttct tatgttttcc atccatattc tatggtaaaa gaccatcgga
    15421 caaacgtaga gacaggaaat gtccaagcag ttatggacgg tgaccttgat ttatttattg
    15481 acggctattt aaaaatgcat ctacaataag aacccattgt taaattgaaa taaaattgta
    15541 agattttgca agcatgttga aacattgcca tgctataata gctaaagact gaaaaagata
    15601 tggagagaac gccatgattg aaatgaagga tgtaatgaag aagtactcaa atggtacgac
    15661 cgctatccgc aatctctcaa ttgtcatcga acaaggagag tttgtctatg tggttggtcc
    15721 gtctggagcc ggtaaatcta cattcatcaa attgatgtat cgagaagaaa aagcttcaaa
    15781 aggaactttg aacgtcgcag gtcatgatct aatagcaatc aaaaaccgcg aagttcctta
    15841 tctacgcaga gaaatcggcg tggtgttcca agactataaa ttattaccaa gaaaaacagt
    15901 atatgaaaat gttgcgtacg ctatgcaagt tatcggcaga aaacctcggg atatcaaaaa
    15961 acgagtaatg gaagtgctgg accttgttgg tttgaaacac aaagttcgag tctttcctag
    16021 cgaattatcc ggtggggaac agcaacgtgt ctctattgct cgggcaatag tgaatacgcc
    16081 taaagtactt atcgcggatg aaccgacagg aaatctggat ccagaaaact catgggaaat
    16141 catgaaatta ttagatcgga tcaatgccca aggaactact gtcgtgatgg ctacacataa
    16201 cagtacgatc gtaaatacga ttcggcaccg agtaattgct atcgaaaacg gccgcattat
    16261 tcgagatcaa gcggaagggg aatatggata cgatgattag aacctttttc cgccatttat
    16321 tagaaagtat caagagtttg aaacgtaacg gctggatgac agttgcttca gtcagtgcgg
    16381 taacgatcac attgacttta gtcggtgtat tcatggcagt tatcatgaac gcaactaaat
    16441 tagctcaaga tatagaagga aacgttgatg tctctgtatt cgtagatatc ggtacaaaag
    16501 acgaagagat gaaaaagcta gaaaaagaat tgaatgactt agatcatgtt aaaaaagttg
    16561 aattttcaag caaagaacag gaattgaaaa aaatccaaaa cgaaatgggt gattcctgga
    16621 aattgtttga aggtgacaat aacccgttat atgatgtgta tgtagtaagt gctactgatc
    16681 cttcttatac aaaacaagta gcagaagaag cagcagattt gaagaatgtt tctcgttctg
    16741 attacggtgg agcttcctca gatcggattt tccaaatcgc tggtgcagtt cggacatggg
    16801 gattagttgc agcggcattg ctgctgttcg tagcgatgtt tctcatttcg aacacgattc
    16861 ggatcacgat tttgtctaga cagcgtgaaa ttcaaatcat gcgtttagtc ggtgcaaaaa
    16921 atggatatat tcgctggccg tttttccttg aaggtggctg gatcggtctg atcggtgcga
    16981 ttcttccgat tttgatcatc acatttggtt atgcatggtt tttccaattg atcaatccat
    17041 cattattacg ttcccattat tcattgattc atccacaaaa tatcgtttgg aaaatcaatc
    17101 ttttaatggt cggcattggt gtgatcattg gttcacttgg ctcgatcatt tctatgagac
    17161 gtttcttgaa attctaatac cacaatataa ataaaatgag aaagttggac agtttcgttc
    17221 cagctttctt tttttattgg ctaggagaat agaaggaagc ggcttttttc cctttacttt
    17281 aaaaaacaac ttgggtattc ttaagttggt agaagaagag gtgaagccgt gaaaattatc
    17341 gttgacaatt tttttacgat ccttttgatt atgaatattt tgctttcctt tatcatcgtt
    17401 tttcgagaaa gaaaggaaac tgctcaaaca tgggcatggg tgctcgtttt gatgttcatt
    17461 ccagttgtag gatttatttt atatattttt ttaggaagag ggatttccaa agagaaaatc
    17521 tttgatttga agatccaagg gaaaatcggt aagaacctgg aaattgaaga agacaaacag
    17581 gcattgatgc gagggcttta tcctcatccg cctactggac aagtagatgt gaaacaattg
    17641 atctatatgc tcacggtatt tgaaagtacg ctctatacga ctaaaaatga aatcacttta
    17701 tttacagatg gtcgtgaaaa attcgatgcg ctgattgaag atatcaagca ggcagaagac
    17761 catatccact tccagtatta tatttatcgc agtgatgcat taggtgaaga agttcgagat
    17821 gcattgacag aggcggcaag acgaggggta aaagtcagag tccttcttga tgcatggggt
    17881 tccacacaag tttcttccag cttttttcag aatttgaaaa aagcaggcgg agaagtcgca
    17941 tttttctttc ctctatttgt tccgtatatt aatcctcgga tcaattatcg caatcatcgt
    18001 aaaatcgtag ttattgatgg gaaaatcgga tatacaggtg ggttcaatgt tggaaatgaa
    18061 tatctaggat tggtaaagaa atttggttat tggcgagaca atcatttgcg tatttacgga
    18121 gaagcggtgt ataccttgca aaaccgtttt ttgatggact ggaattctca gcatacaaaa
    18181 gaagtaggct attctcccaa attttttccg tccattcact ctactggtga tatagctgtt
    18241 caaatcgtga caagcggacc agataccgaa catgaacaaa tcaaaatgac ttatttgaaa
    18301 atgatttcgt tagccaaacg ggagatattg atccaaacgc cttattacat accggatgga
    18361 tcgattcatg aagcactgaa gttggctctt ctttcaggcg taaaagttca tatccaaata
    18421 cctaacaaac cagatcatct tctcgtttac tgggcaacgt attctttcgc tgcagagcta
    18481 atcgagtatg gagcaaggat cgaaacatat gagaatggat ttattcatgc aaagacgatg
    18541 attatcgatg gtggcattgt ttcagttggc tcagcaaata tcgatgttcg ttcatttcgc
    18601 ttggactttg aggtcaatac actggtttat gatgaacgaa tggcttcacg tgtaagaaaa
    18661 gctttttttg aagactcgaa aatttctacg catctcacaa aagaaatgta tgaaaaccga
    18721 ggaataatca tcaagatgaa agaaggatta gcaagattga tttcgccgct cctttaaaac
    18781 ataaaaagga catgagaacc atcaacagat ggagatctcg tgtccttttc taacggggcc
    18841 atttctgaga gtacgtcaga aatggagaaa gcgtctcgca tgagcgaaat taactttggg
    18901 gtatctcttg ttttttgggg aaagcaagaa ataagctaat atatgctatt aaaagagaca
    18961 catttcaaag ccagtatagc atagaaactt ttaatgagat agtttttttt acaattatga
    19021 gaatttattt tgttatttaa tgattttgat tcgttttcat caagtaatga aaatgataaa
    19081 ataaacatac tttaggatta aaggagattt ctaatgaaaa aacttaaatt attcttacca
    19141 ataataggat tagctattct tttatcaggc tgttcaaagt ggattgatcg aggagaatcc
    19201 attacggctg ttggctcttc tgctttacag cctttagtag aaacagttgc tgaaacttac
    19261 cagacagaaa atcctggaaa attcatcaat gtacaagggg gcggaagcgg aactggtttg
    19321 tctcaagtac agtcaggagc agtcgacatc ggaaactctg atttgtttgc ggaagaaaaa
    19381 tcggggatcg atgcctcagc attagtcgat catcgagtag cagttgttgg gatcactcct
    19441 attgtgaaca aggatattgg cgtaaaagac atcagtatgg aaaacctgaa aaaactattt
    19501 acaggcgaga tcaaaaactg gagtgaagtc ggtggaaaag atcagccagt agttatattg
    19561 aaccgcgctt caggaagcgg aacccgtagc acgtttgaaa aatgggtatt ggacggagaa
    19621 acagctgttc aggcacaaga acaagattca agcggaatgg tgcgccagat cgttgccgac
    19681 acacccggtg caatcagcta tgttgctttt tcttatgttt ccgatgatgt agatacgttg
    19741 aatgttaacg gtgtagaacc gacagatgaa aatgtgacaa cgaacaaatg gacgatttgg
    19801 gcatatgagc atatgtacac aagagatcat ccaaaaaaac tgacaaaaga atttttagac
    19861 tatatcttat ctgatgaagt acaagataac atcgttggag aactaggtta tatccctgtt
    19921 tctcatatga aagtcgaacg tgattggcaa ggaaatatcg ttaaagagta atctttacac
    19981 aaaatttaca aacttagcta aacttttaca tgttattaaa accaaattga tgttcaattt
    20041 gtacactgag tgagtatgtt aaacctgatt aagggggagt catctgtgga agatgtgaaa
    20101 aaagtattat taacaaaatc aaaacgttca aaaatggaac agcgtggaaa attcatcagt
    20161 tttttatgta tcgcgctgat cgtgttggtt gtattatcta ttttttattt tgtagctagt
    20221 aaaggattgg ccaccttttt tactgataag atcaatgtat ttgattttct gtttggtaca
    20281 gattggaatc caagccaaat cggagcaaat gggaaaccaa tggtaggggc attgcctatg
    20341 atcacaggtt cgtttattgt gaccttttta tctgctatcg ttgccacgcc atttgccatt
    20401 ggtgcagcag tattcatgac ggagatctct cctaaaaacg gaacgaagat cttacagccg
    20461 gtcatcgagc tgcttgtagg tattccttct gtcgtttatg gttttatcgg tttatcagtt
    20521 atcgtgcctt ttgtcagaag tatttttggc gggactgggt ttggtatctt agctggtaca
    20581 tttgtgttgt tcgttatgat cttgccgact atcacaacga tgactgttga tgcattaaag
    20641 gcagtgccac ggcactatcg ggaagcttca ttagcgcttg gtgccacaag atggcaaaca
    20701 atctacaaag tcgtgctacg agcagccgtg cccgggattt taacagcagt cgtttttggt
    20761 atggctcgtg cgtttggtga agccttagct atccaaatgg tcatcggtaa tgcagcattg
    20821 atgccaacag gtcttactac tcctgcttct acattgacgt ccatcttaac aatgggaatc
    20881 ggaaatacaa tcatgggaac agttgaaaat aatgtgttat ggtcattagc gctgatcttg
    20941 ctattgatgt cattattctt taatatcgtg atccgtatga ttgggaagaa aggagagctg
    21001 aaataatgaa cgcaaaacgc tcagataaaa ttgctacagg tgttttatat atagtttcgg
    21061 ggatcatcgt attgatttta gcagctttac tactttatat cttagttcgg ggaataccgc
    21121 acatttcatg gtccttcttg accgaacctt ccaaagccta ccaagaaggc ggtggaatcg
    21181 ggatccagct atttaactcg atttatttgc ttttgatcac gatgatcatc agttttccta
    21241 tttctttagg tgcgggtatc tatctttcag aatatgctgg aaaaaactgg ctaacagatg
    21301 tgattcgcac gtcgattgaa atcttgagct cgttgccttc tgtcgttgtc ggtttattcg
    21361 gtttcttggt tttcgttatt caattccagt atggtttctc gattatttct ggtgcattgg
    21421 ctttgactgt attcaacttg cctcttttga ctagaacagt tgaggagtct ttacaagcgg
    21481 tccattacac acaacgagaa gcaggattgg ccttaggttt gtcacgctgg gaaacagtga
    21541 taaaagtggt ggttccagaa gcgacaccag ggattttgac tggtgtgatc ctaagttctg
    21601 gaagaatttt tggtgaagca gctgcattga tctatacggc tggacaaagt gcacctgcgt
    21661 tagattttac gaactggaat ccattaagtg tttcaagccc gatcagcatt ttccgtcaag
    21721 ctgaaacatt agctgtccat atttggaaga tcaatacaga aggcacgatg cctgatggtg
    21781 tttctgtttc ggctggcgct tctgccgtgt tgattattgc tgttttactg ttcaacttcg
    21841 gcgctcgtgc gattggcaaa cgattatacc gaaaaatgac ttcagcctaa ggagaagaag
    21901 caaaatgaaa aaatataact ggaatgaaac aaatgtcatc accatgccaa aagagaaaga
    21961 agtagcacta tatacggatg atctacatgt ctggtatggc gacaatgaag caatcaaagg
    22021 agtggatctg gaatttgaaa aaaataagat cactgcactg attggtcctt ccggctgcgg
    22081 aaaatctacg tatcttcgtt cgttgaatcg aatgaatgat gggatcgcta atacgaaaat
    22141 cactgggaaa atcatgtatc aaggcgtaga tattaatgcg cctcaagtgg atgtttatga
    22201 gatgcgcaaa cggattggca tggtttttca acgtcctaat ccgttcagca agtcgattta
    22261 tgacaatatt acatttgctt tgaaacagca cggcgaaaaa aacaaaaaga aattagatga
    22321 aatcgttgaa acaagcttga aacaagctgc cttgtgggac caagtaaaag acagcttgga
    22381 caaaagtgcc ttggctttat ctggcgggca acagcaacgt ttatgtattg ctcgtgcgat
    22441 cgcgatgaaa cctgatatct tgctgctaga cgagccggca agtgctttgg accctatttc
    22501 gacaggaacg gttgaagaaa cacttgtaaa cttgaaagaa gactatacga tcatcatcgt
    22561 gacgcataac atgcaacaag cagctcgtat cagcgactat actgcctttt tctatttagg
    22621 aaaagtaatg gaatatgaca aaactaacaa gatatttacc agaccaaaaa ttcaggcaac
    22681 cgaagattat gtgtccggtc attttggtta agacggaggc ctcaacatga caaaagagat
    22741 tatttcatct aaagatcttc atctgtatta tggaaagaaa gaagcactaa aagggatcga
    22801 tctaagcttc aatcaagggg aaatcactgc gatgatcggt ccttctggtt gcggaaaatc
    22861 cacttatctg cgttctttga accggatgaa tgacctgatt ccgaatgtaa cgatcaccgg
    22921 aagtgtttta tataaagaca aagacatcta tggtccaaaa acagatgttg ttgaactaag
    22981 aaaagaaatc ggcatggtat tccagcagcc aaacccattt cctttttcta tctatgaaaa
    23041 tgtcatctat ggattgaaat taaaagggga aaaagataaa aaagtccttg atcaagtagt
    23101 tgaagaaagc ctgaaggcag cgtctgtttg ggatgatgtg aaagacaaat tgcataaaag
    23161 tgctttatct ttatccggtg ggcagcagca gcgtgtatgt atcgcacgtg ttttggcagt
    23221 taatccggaa atcatcttat tggatgaacc aacaagtgcg cttgatcctg tttctactgg
    23281 gaaaatcgaa agtatgctgt tagaattaag agagcggtat acgatgatca tcgtgaccca
    23341 taatatgtct caagcttcac gtatttcaga taagacggca ttctttttag atggtcactt
    23401 gatcgaatac aatgatacga agaagatatt catgaatcca gaaaaacaag agacagaaga
    23461 ttatatttca ggtcgatttg gataaggagg aaacggaatg ctacgtacac aatttgaaga
    23521 agaattgctg aatctccata atcagttcta cgaaatgggg atgatggtga gcagcgccgt
    23581 gcacaagtca gttcgtgcat acatcaaaca cgataaaatc ttagcccaag aagttatcga
    23641 aaacgatata aatatcaata acatggaaac acgcttggag aagaaaagtt ttgaaatgat
    23701 cgcattgcaa cagccagtta caactgattt gcgtatgatc attacggtca tgaaagctag
    23761 ctcggattta gaacgtatgg ccgatcatgc ggtgtctgtt gctaaatcca cgattcgcgt
    23821 aaaagggcag acgagaatac cggaaatcga aaaagagatc tccgatatgt ccgattatgt
    23881 gaaaaaaatg gtggacaacg tcctagttgc ttatgtcaaa acagacgaaa aagacgcacg
    23941 tacgatcgcc aatatggatc aaagagtcaa tgactatttc aatcgaatct ataatgcaac
    24001 aatcaaaaat atgcaggaaa atcctgagac agtcatcagt ggaacagact acttgaatgt
    24061 tgccagttat ctagaacgga tcggagacta tgtgacaaac atctgtgaat ggatcgtcta
    24121 cttggctaca gggaaaatca ctgagttaaa tacgaaccac aatgagattt tctaaactga
    24181 agctgtgatt atgccgtttg tttaggaaaa gaggttgtga aatcagcggt tgtgaaaaag
    24241 ctgttctgca tcaagtaata agaacagtca aacaaaaaat agctcctcat atttttcgtt
    24301 tggctggact tattaccgca gatgcaagct tttgaacacc gtttattcgg gaaagggttt
    24361 gtgacaaaac atgtcacaaa cccttttcat catttcatag aaaggataaa gaatgcaaaa
    24421 aaaaattagc atcatcacta tattttttat ttgtgtgctt acttcatggg ttattcagca
    24481 tcaagccgct ttatatgaag tacctgtcat cgagatagaa aaagtacgaa taaaagagga
    24541 tcatcaattg attacaggaa aactgatgaa cacggatcaa aaaggagaat cgatttattt
    24601 atcggttcct tatcaaaaaa atcaaataga aacagcaaat ataaaagcga gacaaatgta
    24661 tctagtccaa atgcatggag atgaatggca gctagtagca aaaaaaaatg atggctggct
    24721 tttcttcttc acaagtatct tcattggcgc catgttgctt attggtggaa aacagggtct
    24781 atttgcttta ggaagtctca tcatcaatat cgttttactt ttacttttgt tgctcatctt
    24841 tactaaaaca gaagggattt ctctattaaa tatatctatc ctgttcgtcg ttatagggat
    24901 gattattagc atattggctt tagatggatg gaatagaagc agtataatca aaatttcagc
    24961 tgcagttacg agtgtttttt tagcattttt tatttgtctt ttgacaatga atcattggaa
    25021 agaccaagga ctgcgctatg aggaactgaa ttatctgaca cgaccgtatc gttctgtttt
    25081 tctttctagc gtactgattg gaacagctgg aggaacactc gataccgtta tcacagtcat
    25141 tgcgacatta gaagaattga ctaagcgaca accagaaata acagcgaaac agttatggct
    25201 ttcaggaagg caagtgggtc gagatgtgat tggctcgatg accaatatcc ttttgttcgt
    25261 ctatatcagt gggtcagttc catcgcttct tttatattta ggaaatcaat ggtcgttcaa
    25321 agaaacgata gaacagcatt tatcattgga atttttacgc gtcattgctg gcagcttagg
    25381 aatagtatta tcgatcccta tctcggtcct cttttttctg actgtaagga ggctgaaacg
    25441 atgaatgtac ttattatact tgttgggatt ttagcaatca gcgtgctact tattggcggt
    25501 acgcaaggta tgtatattct tttaggctta ttcatcaatt tagggatctt tttcctgttg
    25561 ttattcggtt atcatcagaa gtggccgata ctggcattgt cggtcatagg atttttattg
    25621 attgctgtgg ttatcttatt ttttatcaac ggttataatc taaaaatgcg tgcagctttt
    25681 gtgagtatac tgatcttttt attttgtttc ctactattgc ttcctatcac tgatttttta
    25741 gcaatccaag gttttacatc ggtagaacta gaagaattat ctggcttaga taagacttta
    25801 gcgattgatt ttagattact tacgagatcg ttgcttctga ttagtcttag tggtgctgtt
    25861 ttagatgcga gtgtagcaat ttctagtggg acattcgaag tatatcaggc aaatccgcaa
    25921 ttatcttttt accaattgcg ccattctagt ttcgctattg ctaaaaaaat attggcgtca
    25981 acagtaatga cacttctttt tgcattcatg ggaagttctt tagcgttgat cctctggttt
    26041 attgatttag acattccttt tcagcaagtg atcaatgaaa aatcatttgt tctagaatat
    26101 acgatggcta ttcttaccac attgtccgca ctacttgttc ttcctttaac ctgcgtgact
    26161 acagcatatc tctttacaaa aaagaaagaa caactcaaga tggagtaaaa gttacaactt
    26221 aagtcctatg acatttttac tgaatcagtg cataatatgg atgtaaggaa aagcttgcaa
    26281 acaattttgc ttaaggaggc aatttccatg aaagaaagag aacgtatttt agatttagtg
    26341 aaaaaaggaa tcctttcgac tgaagaaggc ttagatttat tagaaagcat ggcaactgaa
    26401 aaagacgaaa agcaaatcaa aaaagaagca gacaaggtaa ctgctagtca taaagaaaaa
    26461 gatcaggcaa gtcaattgat cgataaactt gaaaacggcg aagaagaaat ttctgagcca
    26521 gttgatccaa aggaaagaga aagacaagat caagaaaact tagaaaagat cttagatgaa
    26581 ctagcaactg aagcgaataa aacttcggcg cgtctagatg aagtaaatgc tcaattcgct
    26641 gacaataagg cagcacgcaa tgagaaacag gaagcattga tgcagctgaa tacaaaagaa
    26701 gaattaggcg agttgacaga agaagaatta gcacagcgtc agacgttaga agcagaaatc
    26761 aaagaattag aagctagtgg cgatacactg ttagaagaac aattgaaatt agaagcagaa
    26821 ttaaaagata tccgtaagaa tcaatggtct gagaaaaaag aaacgtttac tgataagttt
    26881 gaattgccag atgattggaa agatcaagca acagatacac tgaatcaagt aggcgaaaaa
    26941 atgtcagaag ctggatcaca gttaggcaaa ttcttgaaga aaacattcca aactgtttca
    27001 gaaacagtga atgacaacat ggaatggaaa gatgtcagct tacgtgtccc tgggattgcg
    27061 acaacgaaat ttgagcatga attttactat gaagctcctg cagcaagtat ccttgatatc
    27121 aaggcagcaa acggaaacgt gacgttaaaa acgtgggaca gcaacgatgt gaaagttgaa
    27181 gctaagatca aactttacgg aaaaatgggt gctgaaccat ttgaagcatt ctctgaacgg
    27241 agccagattg aggttaatga agatcatatc tccttccaaa ttccaaataa acgtgtgcgt
    27301 gcagaccttg ttttctactt acctaaacga gtatacgacc acgcagctat taaacttcta
    27361 aatggcaaca tcatgattga aacattggaa gctaaagata tttatacaaa atctacgaat
    27421 ggtaacatca tcgtggacca attaactgca acgatgctgg aagtagaagg tgtaaacggc
    27481 aatatcgata ttcgaaacgg gaacatctta gattctatta tcgaaacagt aaatggtaca
    27541 gtgacatttg gtgcgacagc agaaaacctt tctgtgtctt tggtaaatgg tgatgtacgt
    27601 cttacgatta aagaagataa tttgaaaaaa gtagaagcaa gttcagtgaa cggaaatgtc
    27661 aaagtcgctt taccagatgg catcggtttg gaaggacatg ctaaaacaag tcttggaagt
    27721 atcaacagcc gtctgtccaa ttacgaagta gttcgtgaga aaaaagaacg gacaaaccaa
    27781 atgttgcaat tccgtcgtgt ttcagatggt gaaatcgcgc aagtgcaact ttcaacgaca
    27841 acaggaagta tctatttaaa agatacagat aaataaagag gttgtgaaag agatgttcag
    27901 ccttgagcaa taagaagcga ttttgtaaaa tagcttcccc tattttacaa aattgtgcct
    27961 tattgccgat agtctatctc ttgaacaccg tttaccaaga gattatgaac ctgccaatta
    28021 tcgccgagtc acaaggaata atttcagaaa aagctcgtca cgttttatga atatggtaat
    28081 ttgtgaccga gaattttgca gaaagacacc gattaagaag aaggttgtca caacgatttt
    28141 cttcatttaa aatagtagaa cagaggtgag acatgatgaa aaaaaagttg acaaaatcta
    28201 ataaaaatgt tgttttgaca gggactttag caggaatagg agaatacatc gggattgatc
    28261 cgactgttat ccgtgtgctt tatgtattcc tgagcttagt tgcttttgga agtccaatta
    28321 ttttatacat tttattagct ttgattatcc catttgcgcg ttctgcacga acaaactatg
    28381 gacataacaa tgcatattat gaaaaaaaca attatcgtga acaaactcgt gaacaaacaa
    28441 aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg gagtgacttt taatgtcgta
    28501 ctttcaacgt attttagtta atacgttaac attcatatca ttggctgttc tttttcctgc
    28561 gatggtccat gtgagcagta tttggacggc cgttcttgcc agcttcgtat tgtctgtgtt
    28621 gaatatgttt gtcaaaccga ttttgacgat actgtccctg ccctttacat tagtcacatt
    28681 gggattgttt acgtttgtta tcaatgcagc gattcttaaa atgacctcct tttttgtggg
    28741 agcagaacat tttagttttt cttcattcgg tgcagcgttg ttagtagcta tcttgatgtc
    28801 gcttgtcaat gtaattgtca gcgaacataa cagtagaaaa taaaagaaag ctgtgccgaa
    28861 ctatcggcgc agttttttta tttagaatca ggaatgcgtt ttctattaca ttggtaatta
    28921 aataaaaacg ttttttttaa ttggtagtac ttttggctag acaatgataa aatgatagag
    28981 aacactctaa tagaaatgag gtagcatgaa tgtcagaagt tgtaaaagtt agccaattag
    29041 tgaaaaaatt atctttggaa atcgtgacag gcgacgagaa aagcttagac cgtgtcatcg
    29101 taactgggga catctcacgc ccaggactag agttgactgg ttattttaat tattattccc
    29161 ataatcgttt acagctattt ggcagtaaag aaatcagttt tgcggaacgg atgatcccag
    29221 aagagcgttt gatcatcatg agaagattat gtagtgaaga tatgcccgca tttattattt
    29281 caagaggatt accagctcca gaagaaatga tccaggcagc aaataaaaat gggatagctg
    29341 tactgcgttc tcccatctca acttctcgtt tattaggaga attatccagt tatttagatg
    29401 gtcgccttgc ccctcgaaca agtgtccacg gtgttttagt ggatgtttac ggattaggtg
    29461 tactgattca aggtgacagt gggataggaa aaagtgaaac agcgttggag ttgatcaaac
    29521 gcggccatcg attgatcgca gacgatcgag tagatgttta tcagcaagat gaattaacgg
    29581 tgatcggaga accgcctaaa atcctggaac atctgattga aatccgagga atcgggatca
    29641 ttgatgtaat gaatcttttt ggtgcgagtg cggtacgtgg ctctatgcaa gtccagttgg
    29701 ctgtttatct ggaagcatgg gcaaaagata agaaatacga ccgtctgggc tctgaagaca
    29761 cgaccgttga aatcgcggaa gtgggcattc cacaaataaa aatccctgtc aaaacaggac
    29821 gaaacgttgc aatcatcatt gaagtagccg cgatgaattt ccgtgcacgt acaatgggct
    29881 ttgacgcaac gaaaacattt gaagaaagat taagtcgctt gattgaagaa aactcaggca
    29941 acgactagca aggagtgaca atatgttagc acaaatcaat cgagttgctt ttgagcttgt
    30001 agggattcct atctactggt atgcattgat catcgtatca ggtatcatta tcgctatgtg
    30061 gcttagcagc agagaggctg ttcgggtagg attaaaagca gatgatgtga cagattttat
    30121 gttgtgggga ttgcctatct ccattattgg cgctcgctta tattatgttt tatttgatct
    30181 tccccagtat atagctgatc caatccaaat attcaatatc cgttcaggag gcttagctat
    30241 ttatggcgga ctgattgcag gcgcgatcac gctgtatttt tttactaaat atcattttat
    30301 ttctttgtgg acttttttag atatcgctgc accaagtgta ttattagcac aggcaatcgg
    30361 tcgttgggga aatttcatga accacgaagc atatggacca gacacgacgc gaagcttctt
    30421 agaaagtctg catttaccgc aattcatcat tgataatatg tatatcgaag ggatctatcg
    30481 tcaaccaaca tttctttatg aatcactttg gagtttgatt ggatttattt tgcttttgtt
    30541 tttacgaaag aaaccacacc ttttgaaaaa aggcgaagtc cttttatgtt atctaatgtg
    30601 gtatagtttc gggcgtttct ttattgaagg attacgtaca gacagtcttt actttttagg
    30661 tgtgatccgt atttctcaat tattatctgc tgtgttattt gtcggtacat tgattctatt
    30721 tatttggcgc agaaaaaaag gaaatttacc ggattatgac cgctcattag gaaaaaatca
    30781 acaaattatc tgatttttag agcgttaaaa atgaaaaaaa tttcaggagt ggtaaaatga
    30841 attatagagt agcggtttta ggaccaggct cttggggtac tgctttggca caagtcctcg
    30901 ccgaaaacgg acatgacgtt cggatttggg gacatcgtgc ttcacaagta gaagaaatca
    30961 acacccaaca cacgaatcaa cgttatttac cagatcatta tctacccgat tccatccaag
    31021 cattcaccaa tatggaagat gcagtaaaag acgcagatgc ggtgctattc gtcattccaa
    31081 cgaaagcaat acgttctgtt gcaaaagaac tagtgcctaa attgaataca aagccagtga
    31141 tcatccatgc aagtaaagga ttagaacagg gaacacataa aaggatctct gacatcttgt
    31201 tagaggaaat cccagcagaa aaaagaaaag ctgttgttgt tctttctgga ccaagccatg
    31261 cagaagaagt agcaaaacat gacatcacta cgatcacagc tgcaagtaca gatgcaaaag
    31321 cagcaaaata tgtacaagag ctgttcatga ataattattt tcggatctat accaatcctg
    31381 atgtgatcgg tgtagagatg ggggcagctt tgaaaaatat tattgctatt ggtgctggcg
    31441 ctattcacgg cttagggttt ggcgataatg caaaggcagc aatcatgacg cgcggattag
    31501 ctgagatcag ccgtttaggt gtagcaatgg gtgccaaccc gctgaccttt atcggattaa
    31561 gcggaatagg cgatctagtc gttacttgca caagtgttca ttctaggaac tggcgcgcag
    31621 gcaacttgtt aggacaaggg cagcccctag aagaggtgct agccaatatg ggaatggtcg
    31681 ttgaaggtgt gaatactacc caagcagcga tggaactagc ccaaatgatg gaaatcgaaa
    31741 tgcccatcac tcaaacaatc tatgaagtgc tgtatgaaca aaaagaaatc aaaacagccg
    31801 caaaagaaat catgttacgt gatggaaaaa aagaaaatga attctcatta acaatttttt
    31861 gaaggagcga atgtatatga aagttaaaaa agcagttatt ccagcagcag gattaggaac
    31921 aagattttta ccagcaacaa aagctatggc aaaggaaatg ttgccaattg ttgataaacc
    31981 aacaatccag ttcatcgttg aagaagcgtt gaaatcaggt atcgaagaca tcttgatcgt
    32041 tacaggaaaa gctaaacgac caatcgaaga tcactttgat tcaaatattg aattagaaac
    32101 aaacctaagt gaaaaaggaa agaccgaatt attgaaatta gtagaagaaa cgacagatgt
    32161 caacttgcat ttcatccgtc aatctcatcc aaagggttta ggacatgccg ttcttcaagc
    32221 aagagccttt attggaaatg aaccatttgt cgttatgcta ggcgatgata taatggaaga
    32281 cgaagtacca ttgacacagc aattaatcga tgattacgaa cgtactcatg cttctacgat
    32341 cgccgttatg caagttcctc atgatgaaac ttcaaaatac ggaattatca atccaggaga
    32401 agtattggaa gatgggttat acaatgtcaa aaactttgtt gaaaaaccag atccaagcaa
    32461 agcaccaagt gatttagcta ttattggacg ttacttattg actccagaaa tttttgatgt
    32521 attgtccgag caacaaccag gagccggaaa tgaaatccaa ctaactgacg caatcgatac
    32581 attgaataaa acacaacgcg tatttgcccg taaattcaca ggtaaacgtt acgatgtcgg
    32641 tgacaaattt ggttttatga aaacaagtat tgaatatggt ctaacacatc cagaagtagg
    32701 tgcaccttta cgtgagtaca tcctgtcttt aggtaaaaca ttagaagcag aaggaagcaa
    32761 gatcgatcat aatccaaaag gaataacaga aatcaaagaa gaaaagaaaa aataaccctt
    32821 ttttctaacg agcttcagct gctaattgaa taaaaagtag ttgaagctcg tttttatttg
    32881 tttaaaaaac acgaaaatgc tatccttata gaaaagagag acaagaaggg agtttgatca
    32941 tgtcaggagg agaaattgca ggattgatcg cagcaatcgc ctttgcgata cttgtcgttt
    33001 ttatcgttcg tgtgttgttg caagtttcta aaactttaga aaaagtagat cagactgtag
    33061 cagaagcaaa tacgacgatc gaaatcgtca caaaagacgt agatcttttg tctagacaag
    33121 tagaaggctt actggtcaaa agtaatgaac tgctcttgga tatcaatgga aaagtcgcaa
    33181 cgatcgatcc attgttcaca gcagttgctg atttaagtga aagtgtatca gaacttaatc
    33241 attctagtaa aaacatcgcg acgaaagtcg gcggaatcgg taaaacgaca gctaaagcga
    33301 cggttgccgg aaaagtcggt gggacagcga tgaaattttt taaaaacaaa aaacatactg
    33361 aaacgacagg aggaaaataa aaatggctaa aaaaggtgga tttttattag gtgcagtagt
    33421 cggaggcaca gccgcagcaa tcgccgcgtt attgttagca ccaaaatctg gtaaagaact
    33481 tcgggatgaa ttagctgccc aagcggatga tttgatggat atggcttcag attacacaga
    33541 tatggcgaaa gaaaaatcga ctgagttaac aggtattgca aaagaaaaag cgcaagatct
    33601 ttctcaacaa gcaggtgatc tagcaggtag tgtcaaacaa aaagtaagtg aatcagcaga
    33661 tgaagcagga agtgaatctg acgatatctt gttaaatctt aaagaacaat cagctgactt
    33721 atctaatcgt ttcaaaaaaa cggcagaaga tatgaaaaaa cctgcagatg aattaggaag
    33781 tattgctaaa gatacttcag aagatatcat gattgatgta aaagacgctg ctggtaaagc
    33841 aggagatact gtcaatgaag gaatgtcaga agcaaaacca gttgcagatg actcaaaaga
    33901 aatgatgaaa atggcaaaag aagatgcgaa agaagctaaa aaagaaataa aagatacaat
    33961 gacagattca tctacaaatc actaaaatca caaatttata gaataaaaag gtgggagcgg
    34021 actagctctc acctttttta ctcttatttt tctaaaacaa gtaattcttt tggtgtagtc
    34081 gtgaatggca cacagccatc gcttgtcaca tatacacagt cttcgatacg tacccctact
    34141 ttttcaggaa tatagattcc tggttcaaga gagaagcaca tcccttcttc gattactaag
    34201 tcatttcctt ctacaaggga agggaattcg tggacggttg ttccgatccc gtgacctaaa
    34261 cggtgattga aatattcgcc gtagccatag ctgttgatca cgtttcgtgc gatttgatca
    34321 agttcacctg ctgtcacacc tggacggacg gcttcttgtg cggcaagctg tgcttcaaga
    34381 gtgatgttgt agattttttc ttggaagtcg gttggttttt gataagcaat tgtacgagta
    34441 gcatcactgc aatagccatt ccaaacaacg ccaagatcga ataacacaag ttcattcggt
    34501 gaaatcttcg tatttcctgg tgtaccatga gggcttgcag catttgctcc tgcaaggact
    34561 aatgtgtcga aagacatttg tgaaacacct tgttttttca attggtattc aatttctgca
    34621 atgatcgctt gttcagtcac gccttctttc actgctttaa agccaatctc aaatgcaaca
    34681 tcggcccatt taccagcttc gatcagtttg tctttttcct ctagagtttt gatcagttgt
    34741 agacgttgga tgatggaagt tagatcagtg taagaatccg ttcccgtcaa gatatcagag
    34801 atagcatcga agcgctcgac agtcaaggcg ttcttttcaa tccctacatt gccaggctga
    34861 ccatagcgag cacgaagttc ttttccgatg atctcccaag ggttttcgct gtctaaatag
    34921 ccacggacat catacgccca actactgttt tgagcatctt caacttccaa agcaggagtg
    34981 aaaaggaaag gatcttgttc aagagggata aaaagagcta gtacacgctc atgaggatca
    35041 ctttcgtatc cggaaaagta agcgatgtgg ccagggtcgc tgatataagc caattcgact
    35101 tggtgttgtg ccatccattg acgtagttca ttgattttta cgttattcat ttggcaacct
    35161 actttctaaa ttaattttgt attcaataat tgtaccactg atttattaaa agaccaacgt
    35221 tctaaaaact gaaaaaagta aaaattttca tgaaaacaga gataaaacgg ttgcaattct
    35281 tgagattatt tgatattcta ttttagaaat gtaagacgta ttttcagaaa acatataaaa
    35341 tatgaatagg agaacacaaa tggaaaagca aacaatcact atctatgatg tagcgagaga
    35401 ggcaaatgtt tcgatggcta ctgtttcacg tgtcgtaaat ggcaatccaa acgttaaacc
    35461 agcaacaaga aaaaaagtct tggaagttat cgatcgcttg gattatcgtc caaacgcagt
    35521 tgcgcgtggc ttggcaagta agaaaacaac aacagttggt gtaatcatcc ctgatgtcag
    35581 caatatgttc tttgcttctt tagcacgtgg tatcgatgat gtggcaacaa tgtacaaata
    35641 caatattatc ttagcaaatt cagatggcaa cgaccaaaaa gaagtcaatg tattgaataa
    35701 cttactggct aagcaagtgg acggcgtgat cttcatgggt caccatatca cagacgaaat
    35761 ccgtggagaa ttttcacgtt caaaaactcc tgttgtatta gctggctcta ttgatccaga
    35821 tgaacaagta ggtagcgtaa acattgatta tgtatccgca acaaaagatg cagtaaacaa
    35881 attggcaaaa agcggcaata aaaaaatcgc ttttgtcagt ggtgcgttga tcgatcccat
    35941 caatggacaa aatcgtctga aaggctataa agaagcatta agcgaaaata acctaccata
    36001 tagcgaagga ttgatctttg aagcacaata caacttcaaa gatggtctat ctctagtcga
    36061 tcggatccat aatagcggtg caacagctgt ctatgtttca gatgatgaat tagccatcgg
    36121 tattttggat ggtttattag accgtggcgt gaaagtaccg gaagaattcg aaatcatcac
    36181 aagtaacaat tcactactga cagaagtagc tcgtcctcgt ttaacaagta tcacacaacc
    36241 attatacgat ttaggggcgg tatctatgcg cctattgacg aaaatgatga ataaagaaga
    36301 agtcgaagaa aaaacgatta tcttgcctta tggaatcgaa gaaaaaggat ctacaaaata
    36361 ataaaaggac aaccattcag aggttgtgtg cctgccgttt aactctgcgt tacaaggagc
    36421 aatttcaaaa aacagcatct catgtttgat gaatattgtg actcgtgatt cggagttggc
    36481 aggagaacac cgtttatttg ggaaaagggg ttatgacaaa attttgttat aatccttttt
    36541 ttgtgtctta aaaaagagag aaagtggttg aaattacatt tatcgtatat aaaacaatcc
    36601 gtgtatagat gctgcatttt gttcaccacc atcaaaaaaa taagggactg tgacaatact
    36661 attgtcacaa tcccttatta ctgtgtattc tattaaatca atcatctttc tcatctgatt
    36721 ctttagttgc ttgattcgct cttgcataag ctgaagcggt gttccagtaa tctgtatagt
    36781 tttcatcggt accgccaacg ccaaatttaa aggcgctttt cttcggtcca tttttcgccc
    36841 atagcgattc aactgttttt cctggtgttt ggatggattt tttattgaca gtaacttttc
    36901 caggtgtttg accagtaaat ccagatactt ttttcttcat cacatcagat gataattcga
    36961 atttctcatc agtgccaaaa atgtcaggtt ttgcttggta agcttgattg atcagattgg
    37021 caagataatt agccgaacgt atccttgctt tgctgtccat tccagtattg tcatcgtgcc
    37081 ctgcccagct actgatggta atagacggag tagagacaat tagccatgaa tctcggtatt
    37141 cattggtaga accagttttt ccgacccaat ctgcttttcc tagatttccg tttaagcttg
    37201 agatggcatc tttgaatggt gtagtgattt ttgcattgat cacactacgc atcatgtcat
    37261 tcatgatcga tgccgttgct tccgaataga tccggacaga gttgctttcg tgtttataaa
    37321 ttacatttcc tgcgttatct gtgatggaat cgatgatata gccttcttca tagacgccgc
    37381 cgtttgctaa agcctgaaat ccgttggtct gttgaagcgt cgtgacatct actgtaccta
    37441 aaggagcaga ttcaacgccc caattgtcat ttgccgggta gttcatcttt gccaagtagt
    37501 gctcgtaagc atattgtttc gatccaccgg aattcaacac atcttgatat aagtgataag
    37561 ccgggatatt gttggaccat tctagtgact cacggacggt ttggaaggtg ttcgatcctt
    37621 cgttagtagc attgacgatt ttctcgccag cattttctcc ttcttgccaa gttgttgcat
    37681 aatctgatac acgggattct gatccgataa gccccatgtc gattgcaggt ccataaacaa
    37741 gaactggttt gattgatgag ccagcttgac gttctgtatc gaaggcatgg ttattttgat
    37801 tctgactgta attccgtccg ccaacgaatc cgtagatgcg gccagttctg ttatccatca
    37861 atacatttcc ggtttctact tgactgccag caccatcatc taacatgtat ccataatttt
    37921 caacgccatt ttgcaatgca gcatagacat ctttatcgat tgtcgaatgg attgtatatc
    37981 ctttattttg gatcgtttgc tgtgcctttt gataataagc gtcactgacc gatgaatcag
    38041 acatatcagc gttgtctttt tcagccaatt gttgtgcaat gattcttgtt gcttcattca
    38101 tcacagtata atacaagaat tctctgtcag tctgttctgc gatttgttgt ggtagaaagt
    38161 cttttgtaag gtcataggct tttgcttctt cgtattgttc tttcgtaatt tggccttcac
    38221 gatacatact gaataaaaca aaatcttttc tggcaaggcc ggcagaaaga tcttctttta
    38281 gcgcgcctgt gttcgtgtat ggactatacg tgatcgggct ttggggtaat ccagcgatat
    38341 aagcagcttg cggcagcgtc acatcttttg catctacacc aaagattcct tttgcagctt
    38401 cctggactcc agcaatgttt tgtcccttat tattacgacc aaatggtgag acattcaaat
    38461 acgtagcaat gatctcatct tttgaaaagt atttttctac ttgtgcagaa agaatgatct
    38521 cgtttgcttt acgtttgaat gttgtttcat ctgtaaggat ctgctgtttg actaattgct
    38581 gtgtcaaagt tgatccacca gaagagccaa taccagtagc ttcagatact aatgctcgta
    38641 aaacagcttt aggaacgtaa ccttggtgtt tatcgaagta ttcatcctct gtgctgataa
    38701 ttgcagtttt caataaaggt gaaatcttat cagatgaaat agtcgtgcgc atcagatctg
    38761 tctggatttt tgatatctct gtattatctg cataaacaat ctttgacgtt tctgtaatgt
    38821 ttcctagttc tgtctgcagt gcttttttag tcggcaattg tgtgtcttcg acgagatagg
    38881 caaaatagcc agcgcctatt ccaataccta gagctgcgaa catcaaaaga agaacagtaa
    38941 taaaaacagt caatgattga aaaactctaa aaataatctt aggaacaaac catttttctt
    39001 tttttcttct attctgtttt tttcgagtat ttttgcgtgg caaaagggtc acctcaacta
    39061 atggttttat ttcgctaatt atagcaaata atagaacaaa aaaacagaca aggctgtttt
    39121 tggcaggaat ttctttttta aaagagttaa aacgtgagaa aatgatcttt attataaaag
    39181 agttcttaag cttttcttta gagaaaattt cagttactat ggagaacagg gcaaagaaaa
    39241 atcaatcatt ttctttgtat atcatcttac caatatatag gaattgttag taacaattgc
    39301 ctttcaagga ggaatccatc catgttatta ggctcacacg tcagcatgag cggaaagaaa
    39361 atgctgttag gagcagcagc agaagcagcc agttatggat cttcgacttt tatgatctat
    39421 acaggcgccc ctcaaaacac tagaagaaaa gccatcgaag agatgaatat tgatgccgga
    39481 aaagcgtaca tggcagaaaa cggtctaaca aatatcgtcg ttcatgcccc ttatatcatt
    39541 aatttgggga atacgatcaa accagaaatg tttccttttg ccgtccaatt tctgagagaa
    39601 gagattgttc gtgcggaagc tttaggcgct actcaaatta cacttcaccc aggtgctcat
    39661 gtaggggcag gaacagaagc cggaattgct agaattattg aaggcttgaa tgaagttttg
    39721 acaaaagaac agacggcaca aatcgctttg gagacaatgg caggtaaagg tacggaaatc
    39781 ggtcgaacat ttgaggaatt agcgcaaatc atcgatggag taaaattaaa tgaaaaatta
    39841 tccgttactt ttgacacctg tcatacgaat gatgccggtt ataatattaa agaagacttt
    39901 tcaggtgtaa tggaagagtt tgataagatc atcgggttag atcggcttaa agtactccat
    39961 atcaatgatt ctaaaaatcc gcaaggttcg cataaagacc ggcacgccaa tattggtttt
    40021 ggaacaatcg gattcgacac gctgaataag atcgtccatg atccaatgtt tgacaatatt
    40081 tcgaaaatct tggaaactcc ctatgtcggc aaagataaaa agaaagctta cgcaccatat
    40141 ggaaaagaaa tcgccatgtt caaagcacaa atattcgatc cagaagtgtt tcctgagtta
    40201 ttagcagaag aagtgatact ctaagatttt ctatctgaca gcagcaaaaa aggaccaggc
    40261 acattgcctt gtcctttttt gtttaagaaa caaaggagta ctttttgcag tctttttcat
    40321 catgcatctt ctcccccctc tttttaaatt tagacaaaca aggtatactg ttagaaaagt
    40381 aaaaaaacga aaagtttcac aagcaagtgg caaaacgtga ttggaaggac gaaaagatgc
    40441 tgaacacttt aaaacccttg atcaaccaac taaaaaagaa acatacatac tttattcttg
    40501 gcatagccct attatttaca attattcgtt tgtatttata tagtaaagca atctatggaa
    40561 cagacatgtc ggcaacctat gatgaccagc tgctgattcg ctatgctcaa aatattttgg
    40621 acggtaaatg gctaggggag tatacgacta caacactttc caaaggaatc tcctacagct
    40681 tatttttagt acttgcacat aaactgcatc tttcttatgc tgttttgttc ggcatgttca
    40741 gtatttttgc cagtatattg gttgcactcg cgtttaaacc gatcgtgaag aacaagtatc
    40801 ttcttttaag tatctatttt tattttttgt tttctccgat tagttttaca catgaatatt
    40861 ccactcgaac atatcgtaat gccctcgtga ttccggctgt tgagatcatt atcgcttgtt
    40921 tactagctat ttattaccgc aaatacggtt ctagcagaaa attgctgcca tggtttttag
    40981 ggctttctgt cgcttttcct ttcttttggt tcattcgcga agattctatc tggcttcttc
    41041 cttttactgt agtagctttg ggtatcagtt tcgtccagat cttgctaggg caagactttt
    41101 ctttcaaaga agtaaagaag ttaaaaaaac gactctttct tctctcgaaa cgggaatggc
    41161 tgaaactttc attgttttta cttcctatag ttggcttttc catcgtctat cagtggatcg
    41221 aatataaaaa tgagacaaca tacggtattg caaccatcaa tgaccgttcc agtggagaat
    41281 ttggacgttt gatgaaacaa ctgatccgaa tagatgatga aacgaacttg aataaagaaa
    41341 acagtgagat atgggtctca catgctgcac taaataaagc attagccgca tctcctacat
    41401 tccatactgt ttctacccgt attgacgaat tatatacgac tcacgcgtgg actcaaggtg
    41461 gaaaagtaaa agaattaccc ggagatatca tcttttgggc attgcgagat gtggtaagcg
    41521 aatctggtta ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa aaagtcaacc
    41581 aagaattaga agaagcctat cacaagggaa ccatcaaaaa gaaaaaagag ttctatctga
    41641 tgggatcagg agatggaaag acgatgagtg atctgccaat cgtattcgat ttccttcaat
    41701 caggatacgc ttatgcatgg cgatataaag atttccatca aggaggaaat tatagtttcg
    41761 gaacgcaaga acaagtcaaa caggctcaag aattattaaa ccttccgcta atggagaatt
    41821 ggatcgatag taatgaaccg agaacaccta atttccaatt aacaaaatca gcaaaagtcg
    41881 cgaatgtcat cataaaaatt taccaagttc tggctattcc cgtgctgatt gcttcaatag
    41941 cagggatatt acttatgata ctaggaaccg tatttgcaaa agctgatcgt gcctattata
    42001 gttcctcttt gattgtggtc accggattaa tactgacaca attggttttt cttttcggtg
    42061 tctcgtggtt ttgttcttat gcgccagaaa aaaaggatta ttttttaagt gtctacactg
    42121 gcgcaggcgt gccattaacg caattcatcg tagtaacaag catgctagtt ttcagtaaat
    42181 taaatctgaa aaaggcttta tcttctaaat aaaaagaccc ttggtagcta gatttttaac
    42241 ctaactatca agggtctttg tgtggaaaaa tcagtttcta ttcgatttct tctaccgttt
    42301 cctgcaaacg ctgttcatat tttttgtttc ctttatataa atcaccaatc gtccatgcca
    42361 acaggagaag gacaagaaca atgattacat aagcgatgtg atcagccaat gctagttgag
    42421 cagaagtggc ttgttcacca aagaaacttt cgatctgtcc tggtaatcct cgtaagttca
    42481 agtaagttag actaatcaca gagaaccagc caagaagttt tacgatgaat gaatttttaa
    42541 atcggctacc catctccgct tggctgttgg tcatcatcag taaagggatc atcgaaaacg
    42601 gcaacgcaaa agccaagaac acctgggatt cattcatcaa gttattcaaa gcggtatgtt
    42661 cttcgatcgt tccttttgta cttgtaaaca aaacacaaat caatacagga atcacagaaa
    42721 gcaatcgtgt gaccagtctt cttagccaaa tcgggatacg catatggatg aagccttcca
    42781 tgatgacttg accggtcaac gtaccagtaa tagtagagtt ctggccagaa gcaagaagtg
    42841 ctacagcaaa taaagtggat aagataccag attttgctac agcaatcaag ataccattgc
    42901 tcatagctga tggatcagat aaggcttcat acagaccaaa aaaggaagga tcttggacag
    42961 aaccggtttt gaagacagcg acacccatga tcaatagcag tgcattgact agaaaagcca
    43021 tcgacaattg gatattagag tcccaagtag aaaaacgtac agcttgtgct acatcttctt
    43081 cgtcttggtg atcaatcttc cgagtttgag aaactgctga atgaagatat aagttatgcg
    43141 gcatgactgt tgcaccaata atccctaaag ctccagtcag cggagtttcg ccgccgatga
    43201 ccggtgagtc tgagaatgta tgactgttag ggataaagcc agcaaataaa ctgtgccaat
    43261 ctgggttaga caagccaact tgatagacaa aaacgaataa aatgacgaaa attaagcaga
    43321 caaccagtgc ttcgatctta cgaaacccga cttttgtcag tagtaagaga acgaaaacat
    43381 ctaatacagt aataaagacg gcaatcacca atggaatatc aaaaagtaag tagagggcga
    43441 ttgctgcacc aatgacttct gcgatgtctg ttgccataat ggctaattca gtcaagatcc
    43501 ataaaatgac acccaatgtc ttacttgtgc gtgctcggat tgcttgggcc aaatccattt
    43561 gggtcacgat tcctaatttt gctgccatat actgtaaaag catagcaatc agactagaca
    43621 tcaaaataac tgacatcaat aaatattgga aattttgtcc accagtgatc gaagtggacc
    43681 agttacctgg atccatataa ccaactgcta ctaatgcacc tggtccagag taagcaagaa
    43741 gtgttttaaa aaaacttttc ccctttggta catcaactgt tccatttact tcagctaaag
    43801 ataaaccatt tgtatgttca ataagtcgat gcttatgaaa ttgtttgttg gattgattat
    43861 tcaagaaaaa accacctttc ctatttatct acgattacat tctacacctc aaaaagaaaa
    43921 aattaaagaa aaatattcgg tatgcctaat ttattttcgt ggcgcgtctt gttagatgac
    43981 gaaagaaatt ttgttctcct ctaaaaaaac cactattttt tgcatgaaac taaagaatac
    44041 gcctttcttt tttcaataaa ctttagtaaa ataagtcatt gagagcaatt cggaaataga
    44101 aaggagagat ttatatgtta tcaacaggta ttgtcgtact tattgctatt attgctttgc
    44161 taatcggcgc agtaggtggc tttttcctag cgcgtaaata tatgaaagac tatttagaaa
    44221 aaaatcctcc cgttaatgag gacatgttgc gatcaatgat gatgtcaatg ggacaaaaac
    44281 catctgagaa gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg aaaaaatagg
    44341 ttctgaccca acgacttttt acgggctgat tttttcagtc cgttttttat tttgtaattt
    44401 gttagactat tcagattatt cagcaaaaag acaagcacag acaatcctag gctcttgata
    44461 aagacaaagg aggagtaaaa tgtcgatttt tagaaagtta ggctggtttt ttcgtcagga
    44521 aaagaaacat tatttaattg gggttttctc gctattcgca gtggccttgg tacagttagt
    44581 tcctccaaaa gtgattggga tcatcattga tgagattgcc gataaaaatt tatcagtaaa
    44641 gatcattttc gggtggatct tcctattgat tatcgctgct ttcttacaat atctttttcg
    44701 ttatatctgg cggacgaata tttggggaag tgcagctcgg ttggagaaag aacttcggca
    44761 gcagttattc aaacatttta caaaaatgga caatctcttt taccaaaaat atcggactgg
    44821 ggatttgatg gctcatgcaa cgaatgattt gaatgcgatc cagaatgtag caggagctgg
    44881 tatcttgacc tttgcagatt cagtgattac gggcggaaca acgatcattg tgatgatttt
    44941 gtttattgat tggcgcttaa cactgattgc gctgattcct ctgcctttgc tggccgtcac
    45001 ttcacgagtg ctggggtcga aactccatga tgcttttcgt gattcgcaag aagctttttc
    45061 tagtatcaat gataagacac aagaaagtat cactgggatc aaagtcatta aaacatttgg
    45121 acaagaaaaa gaagatctag cggattttga agaaaagatt gaagatgcaa ttattaaaaa
    45181 caaacgagtc aattttctag actccttatt tgatccattc attaccttga ttatcggtct
    45241 gtcttatgtt gcaacaatta ttttaggtgg aaagtatgtc ttggaaggca cgatctcgat
    45301 cgggcagatg gtctcattta ttagttatat cggtatgctt gtttggccga tgtttgcgat
    45361 tggtcgattg ttcaacgtat tggaaagagg aaatgcttct tatgatcgcg tcaatgaact
    45421 tttgcatgaa aaaacgcata tcgtggaaca actgggtgca gtgcagaccc cggtaaaagg
    45481 cgagctgaat gtagctattt cctcttttaa ttatccgaaa gaagaatcac cagcattaga
    45541 acaaatcaag tttgcaatcc aagaaggaga aacattaggt attgttggga aaactggtgc
    45601 gggaaaaaca acgatcatca aattactgat gcgtgaatac gatgattatc aagggaaaat
    45661 ccaatttggc ggacgttcga tcaaagaata ttcattgaat gctttattag gatcaatcgg
    45721 ctatgtgcct caggatcact tcttgttttc gatgaccatt cgagataata tccgtttttc
    45781 taatccaaaa ctttcagaag aagaagtaga gcatgcggct tctttagcat ttatcaacaa
    45841 tgaaatcaag gaattaccaa aaggttatga taccttagtt ggagaacgtg gagtctcttt
    45901 atctggcgga cagaaacaaa ggatttcgat tgctcgagca ttgatcaaaa atcctgaatt
    45961 attaatatta gatgatgcac tttctgcagt agatgccaaa acagaagaag ctatcttgca
    46021 taatttaaaa gaaataagaa aagagaaaac aactatcatt gcagctcacc gtttaagtag
    46081 cgtgatgcat gcaaaagaaa tcattgttgt cgatcaaggg aaaatcattg aacgaggaac
    46141 ccatcatgag ctgcttgcac taggcggctg gtatgaacaa atgtgggaga aacagcaatt
    46201 agaagcgaag atagaaggag gcgaggcctg atggatgaaa attatcaatc ggaatggaca
    46261 aaatcgattc cacttaaaga acagaaaaag atcatcaaac gactgatgaa gttcgccaaa
    46321 ccttttagaa caacgttcat cgttgctatc ttatttgcgt ttgctctgtc tgtgatcaat
    46381 gtcctattgc caaggatcat ccaaactttt atggatgatc atctggctaa acagtcggct
    46441 acgacacaag ttattttgtt ttttgctggt atgtacttat ttggagtcct agtaaaaagt
    46501 atcatttggt ttttccaatg gtatttgtat tccatggctt cattgaagac ctatcagtat
    46561 attagggtaa aattatttga aaagctgcat acactaggta tgcgttattt tgatcagaca
    46621 ccggcaggat caaccgtttc tcgagtgacg aatgacacag aaacgctatt tgaattttgg
    46681 tacgtctttt tgatggtatt gactgggatc tttgcagtcg tttcttcatt cattgccatg
    46741 tttcaaatca atggagaaat tgccttgtgg actttggtgt tcttgccgat tttaggaatt
    46801 gtcatctggt attaccagaa atttagttct cgtatttacc gaaatatgcg ggaaaaatta
    46861 agtcagctga atacaaaatt gaatgaatcg atttccggca tgcgtattat ccagcagttt
    46921 cgccaagaag ctcgtttagc taaagaattt gaacaagtga acaatgagta tttagacacg
    46981 cgctatgcga tgatcaaaac gaattcgatt ctgttaagtc cgatcatcaa ttttctatat
    47041 gcattggcga ttgcgttggc gttgacttta tttgggatcg atgcgttgca ttctcccgta
    47101 gaagttggac tgatctatgc atttacgact tatgtccagg gattttttaa tccaatgtct
    47161 caaatgatgg actttttgag catcttcaca gatgggatgg ttgctggtag tcgtatcttg
    47221 aagattatgg atacggaaga atatgcacca caacaaaacc ctggagcaga tcaaatgatt
    47281 acttcaggta agattgagtt tcgcaatgta agcttttctt atgatggaga gcacaatgta
    47341 ttaaacaata tctcttttgt tgctaatccg ggagaaaccg ttgcattagt gggacacacc
    47401 ggaagtggga aaagctcgat catcaatgtg ttgatgcggt tttacgaatt ttatgaaggt
    47461 cagatattga ttgatgatca tgacattcga gaatatccaa tggaagagtt acgtaagaaa
    47521 atggggctgg tcttgcaaga tgctttcttg ttttatgggg atatcgcagg aaatattcgg
    47581 atgatgaatc cagctatcac agacgaacag atacgccatg cggctgagtt tgttcaagca
    47641 aacaaattca tcgatgaatt accaaaagga tatcactcaa aagtgatcga acatggtgcc
    47701 agctattcta gtggacaacg acagctgatc acttttgcgc gaacaatcgt gactgatcct
    47761 aaaattctag tattggatga agcaacagca aatatcgata ctgaaacaga aggattgatc
    47821 caagaaggat tggcgaaaat gcgacaagga cggacaacga ttgcgattgc ccatcggctg
    47881 tcaacgattc gagatgcaga attgatttta gttttagaca aaggtcatat agtagaacgc
    47941 gggaatcatg aaacacttct agcgcaaaat ggtttgtatg cagatatgta caaactgcaa
    48001 agtagtggta tataaaagat gttgtgacag tagcattttg acctaagcac tagagtgaaa
    48061 ataaaacata gcttcccata tttttcatct gtgagagtta gtgtcgtagt taccagattt
    48121 aaaaaagacg acgtcagcta gtcagaagtg cctaatagcc cctaagaaag cgttgacaaa
    48181 atcgtttgct ttcgataata taaagaaaac gatacaactt ggatagtgat tgcctgatgc
    48241 aagctaaacc tgatgagtct cccatggttt catggacggc tttttgggtt tttctttttg
    48301 ttttggatac agccctatat atcatggaaa ggagcaaaac atgctgattg agaaagtact
    48361 gaataacaac gtcgtaataa caagggatga aagagatcgg gaaatgattg tgatgggaaa
    48421 aggattggca ttcaaaaaga aggtgggtga cccgattctt gactcatgca tcgacaaaaa
    48481 atttcagttg acaaatgacg gactttcaca acagttccaa gaattggcac aagcgattcc
    48541 tcttgagtac ttagaaattt cttgtgaaat cattgaatat acaaaagagg ctctgcaagt
    48601 ccatttaaat gattctgtgt atatttcatt gacggatcac ttgtatacag ctatcgaacg
    48661 tcaaaaaatg gggatctttg tgccaaatgt attgctgtat gatatcaaac aactgtttcc
    48721 taaagagtat gccattggga aaaaaacagt gaagaaaatc tcggataaat accacatcaa
    48781 gttatcagaa gacgaagccg gatttatcgc attgcattta gtcaatgcac aaggcattca
    48841 aagccagcaa gacaatacct acctgtttac cacaacaata caggatattt taaatatcgt
    48901 ccgctattac tataagatta catttgatga agattcagtc cacttttttc gattcgtcac
    48961 acacctgcgt ttttttctga cacgtgtcct agatggagcg aaatcagaag atggagaagt
    49021 ttctgaggaa ttgttccaag ttattcaaaa aaaatataag gatacgtttg gttgcgttga
    49081 gaaaatagca gactttttaa ccggggcttt cgactaccag atgtccacgg atgaaaaaat
    49141 gtatttaacg atccatactg cgcgtttagt gcatgagaat cgttgtaaat aaataaaaaa
    49201 ggaggacaaa ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct
    49261 tccactaatt aattggaggg aatagaaatg ggaaaatacc aggaattagc agaaaacatc
    49321 gtaaagaata tcggaggaaa agaaaatatc cgtagcttga cacactgtgt gacgcgactt
    49381 cgtttcaaac taaaggagga aaataaagca aaggatgatg ttttgaaaaa gatggaaggg
    49441 atagtcacca tcatgaaaag cggcgggcaa tatcaagttg taatcgggaa tcatgtgcca
    49501 gatgtctatg cagaagtact gacagtagcc gggatttccg atgaagcaga agaagaggtt
    49561 tcttcaagta gtaatctgtt caatcgatta atcgacatat taagtggctg tttccagcct
    49621 tttttaggtg ctttggctgc agcgggaatg gtcaaaggat taaatgcttt gttcttgttt
    49681 ctcaaatttt atactgaagg atcgggtacg tacatcatgc tgaatgggat aggggatgca
    49741 attttttatt tcatgccagt gatcttaggg tacacagcag cgaaaaaatt tcatctgaat
    49801 ccgatggtgg ggctcgtgat tggtgcagca ctttgttatc cgactgtaca aggagcagca
    49861 ctacaaagtg gtttccctga tggcagtcct gcgccttata gcttgctggg tcttccggct
    49921 tatgaaacat ttttgggtat cccatgggtg ggcagcagtt atactagtag cgtcgtacct
    49981 gttatcttca ttatcgcatt tgccggacaa gtccaaaaaa ttgccaagaa atggattcct
    50041 gcagtcgtcc agaccttttt ggtttctttt tctgtgttat taatcgcact tcctgctggc
    50101 tttttgatta tcggtccgat tgtcagtata ctgacagatc tattaagcac gggattccaa
    50161 gcattgatgg acttttctcc agctgtgtat ggtgcagttc tagggtttct ttggcaagta
    50221 ctggtgattt tcggacttca ttggagtatt atccctcttg cgattatgca gctagcgcaa
    50281 aatgggtgga gtcagatgtt agtaggcgtt tatggcgcga gttttgcaca gacagcagtg
    50341 gtattggcga tgtatttcaa gttcaaggat caaaaaatga aggcgctttg catgccagct
    50401 gtgatttcag gaatcttcgg cgtaacagaa ccggctattt atggaatcac cttaccgaag
    50461 aagaagccat ttatttactc gatgattggt ggtgctgttt caggaatcta tttgatggca
    50521 tctggaacaa tcagctacat aatgggcgga ttagggattt ttggggtggt aaactacatc
    50581 acggaaacag acgccagtgg tatgtttcaa gcgtttatcg gcattgctat tgcttcggta
    50641 atcgggtttt cgctgacttt tttcttttgg aaggatgaag caggtgaaac agatgctata
    50701 cagaaaaaat caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccacta
    50761 aatgcagcga aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttaatctac
    50821 cctgaaaaag gagaagtacg tgcccctttt gacggaacag ttatgaccct gttcccgacg
    50881 aaacatgcaa tcggaatggt ttctgaaaca gggctggaat tgctgattca tgtcggattg
    50941 gatactgtgc agttggaagg gaaatatttt gaaagcttag tgcagcaagg agaaagagtg
    51001 agacaaggcg atctgttgct acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt
    51061 ttagaaacac ctgtgatcgt caccaattac acggattatt tggatatttt ggaaaaccaa
    51121 ggggagacgg tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga
    51181 agaatggact aggcttaact attcttttta ttccacttct ttattttcaa aattgaaagg
    51241 ctctttcatg atggctgagc tgatagggcg tttatcgaat ttttgaccga cagaaattag
    51301 atggactaaa ttctgttgat aattttctgc aaaaacacaa ctatataaca cgtctagaag
    51361 ataacaaatt gatgtattga tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt
    51421 cgtaagtcgc aaagcggctg tactcattgc agatagcgtt gaatcaccga tactggtcaa
    51481 agctaaaatt ggaacatcat tttgtttcaa tatttctgcg gtacgaagga tcacattgtt
    51541 ttcacccgta taagaaatca gaatcccgca catatcgctc gtacaattgt acgcttcata
    51601 gaatgattca ccaaaagtgg tgacaattgt gacatccatt ttgatgcgct tcattttcag
    51661 catgaaatct tgagaaatca aagtattcga gttactggcg aagatcacga ttttttttgc
    51721 ctggagcagt aaatatttag ctttttgcag ttggtcatga tgaatgagag aaagtgtatc
    51781 atgaatcgtc atctgttcta aagaagccat tttatttgcg attgtcataa taccgtctgt
    51841 ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc ccttcaagat aggctgtttc
    51901 agctaaaaaa gcattacgaa aactgaccca gccatcaaaa cccatttttt ttgctacacg
    51961 gatgagtgta gacgggtgga cataacatcg ctcagctatc gcttggatgg tcatttcgcc
    52021 aagtgctgga gaatcgctta agagaaactg gatcacttca ttttctgaag gagaaaagga
    52081 gatttctttg attttttcac gtaataacat gtagatacct tcttcttatt tttttacgta
    52141 aattataact tttttg
//
LOCUS       DAYYAG010000006        50203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000006 DAYYAG010000000
VERSION     DAYYAG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.006"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1378)
                     /locus_tag="ACHARQ_000297"
     CDS             complement(14..1378)
                     /locus_tag="ACHARQ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8408754.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1375..2934)
                     /locus_tag="ACHARQ_000298"
     CDS             complement(1375..2934)
                     /locus_tag="ACHARQ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8408755.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2990..5470)
                     /locus_tag="ACHARQ_000299"
     CDS             complement(2990..5470)
                     /locus_tag="ACHARQ_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408756.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5454..5789)
                     /locus_tag="ACHARQ_000300"
     CDS             complement(5454..5789)
                     /locus_tag="ACHARQ_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408757.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5803..6837)
                     /locus_tag="ACHARQ_000301"
     CDS             complement(5803..6837)
                     /locus_tag="ACHARQ_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8408758.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6927..7607)
                     /locus_tag="ACHARQ_000302"
     CDS             complement(6927..7607)
                     /locus_tag="ACHARQ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8408759.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7891..8994)
                     /locus_tag="ACHARQ_000303"
     CDS             complement(7891..8994)
                     /locus_tag="ACHARQ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8408760.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9216..9686)
                     /locus_tag="ACHARQ_000304"
     CDS             complement(9216..9686)
                     /locus_tag="ACHARQ_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8408761.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            10019..11659
                     /locus_tag="ACHARQ_000305"
     CDS             10019..11659
                     /locus_tag="ACHARQ_000305"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8408762.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11813..13135
                     /locus_tag="ACHARQ_000306"
     CDS             11813..13135
                     /locus_tag="ACHARQ_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8408763.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13197..13628)
                     /locus_tag="ACHARQ_000307"
     CDS             complement(13197..13628)
                     /locus_tag="ACHARQ_000307"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8408764.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(13692..14528)
                     /locus_tag="ACHARQ_000308"
     CDS             complement(13692..14528)
                     /locus_tag="ACHARQ_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8408765.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(15270..15896)
                     /locus_tag="ACHARQ_000309"
     CDS             complement(15270..15896)
                     /locus_tag="ACHARQ_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408766.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(16191..16526)
                     /locus_tag="ACHARQ_000310"
     CDS             complement(16191..16526)
                     /locus_tag="ACHARQ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8408767.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(16513..16797)
                     /locus_tag="ACHARQ_000311"
     CDS             complement(16513..16797)
                     /locus_tag="ACHARQ_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8408768.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(16853..18376)
                     /locus_tag="ACHARQ_000312"
     CDS             complement(16853..18376)
                     /locus_tag="ACHARQ_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8408769.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(18369..19544)
                     /locus_tag="ACHARQ_000313"
     CDS             complement(18369..19544)
                     /locus_tag="ACHARQ_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8408770.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(19548..19733)
                     /locus_tag="ACHARQ_000314"
     CDS             complement(19548..19733)
                     /locus_tag="ACHARQ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408771.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(19699..21393)
                     /locus_tag="ACHARQ_000315"
     CDS             complement(19699..21393)
                     /locus_tag="ACHARQ_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8408772.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(21390..21863)
                     /locus_tag="ACHARQ_000316"
     CDS             complement(21390..21863)
                     /locus_tag="ACHARQ_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8408773.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(21932..22087)
                     /locus_tag="ACHARQ_000317"
     CDS             complement(21932..22087)
                     /locus_tag="ACHARQ_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8408774.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(22221..22601)
                     /locus_tag="ACHARQ_000318"
     CDS             complement(22221..22601)
                     /locus_tag="ACHARQ_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8408775.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(22605..22820)
                     /locus_tag="ACHARQ_000319"
     CDS             complement(22605..22820)
                     /locus_tag="ACHARQ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408776.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(22823..23230)
                     /locus_tag="ACHARQ_000320"
     CDS             complement(22823..23230)
                     /locus_tag="ACHARQ_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8408777.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(23496..24971)
                     /locus_tag="ACHARQ_000321"
     CDS             complement(23496..24971)
                     /locus_tag="ACHARQ_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8408778.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(24961..25809)
                     /locus_tag="ACHARQ_000322"
     CDS             complement(24961..25809)
                     /locus_tag="ACHARQ_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8408779.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(25846..26208)
                     /locus_tag="ACHARQ_000323"
     CDS             complement(25846..26208)
                     /locus_tag="ACHARQ_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408780.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(26209..26367)
                     /locus_tag="ACHARQ_000324"
     CDS             complement(26209..26367)
                     /locus_tag="ACHARQ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408781.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(26508..27132)
                     /locus_tag="ACHARQ_000325"
                     /pseudo
     CDS             complement(26508..27132)
                     /locus_tag="ACHARQ_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(27129..27440)
                     /locus_tag="ACHARQ_000326"
     CDS             complement(27129..27440)
                     /locus_tag="ACHARQ_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408782.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(27484..27777)
                     /locus_tag="ACHARQ_000327"
     CDS             complement(27484..27777)
                     /locus_tag="ACHARQ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408783.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            27971..28618
                     /locus_tag="ACHARQ_000328"
     CDS             27971..28618
                     /locus_tag="ACHARQ_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8408784.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            28679..29824
                     /locus_tag="ACHARQ_000329"
     CDS             28679..29824
                     /locus_tag="ACHARQ_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8408785.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(29905..30210)
                     /locus_tag="ACHARQ_000330"
     CDS             complement(29905..30210)
                     /locus_tag="ACHARQ_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8408786.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(30235..30666)
                     /gene="ruvX"
                     /locus_tag="ACHARQ_000331"
     CDS             complement(30235..30666)
                     /gene="ruvX"
                     /locus_tag="ACHARQ_000331"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8408787.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(30663..30929)
                     /locus_tag="ACHARQ_000332"
     CDS             complement(30663..30929)
                     /locus_tag="ACHARQ_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8408788.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(31184..31909)
                     /locus_tag="ACHARQ_000333"
     CDS             complement(31184..31909)
                     /locus_tag="ACHARQ_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8408789.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(32270..32340)
                     /locus_tag="ACHARQ_000334"
     tRNA            complement(32270..32340)
                     /locus_tag="ACHARQ_000334"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32306..32308),aa:Gly,seq:ccc)
     gene            32457..33209
                     /locus_tag="ACHARQ_000335"
     CDS             32457..33209
                     /locus_tag="ACHARQ_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8408790.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            33225..33704
                     /locus_tag="ACHARQ_000336"
     CDS             33225..33704
                     /locus_tag="ACHARQ_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8408791.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(33955..35535)
                     /locus_tag="ACHARQ_000337"
     CDS             complement(33955..35535)
                     /locus_tag="ACHARQ_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8408792.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKKPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(35528..36271)
                     /locus_tag="ACHARQ_000338"
     CDS             complement(35528..36271)
                     /locus_tag="ACHARQ_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8408793.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36447..37223)
                     /locus_tag="ACHARQ_000339"
     CDS             complement(36447..37223)
                     /locus_tag="ACHARQ_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8408794.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(37471..38307)
                     /locus_tag="ACHARQ_000340"
     CDS             complement(37471..38307)
                     /locus_tag="ACHARQ_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8408795.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(38318..39247)
                     /locus_tag="ACHARQ_000341"
     CDS             complement(38318..39247)
                     /locus_tag="ACHARQ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8408796.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            39439..39792
                     /locus_tag="ACHARQ_000342"
     CDS             39439..39792
                     /locus_tag="ACHARQ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8408797.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(39866..40522)
                     /locus_tag="ACHARQ_000343"
     CDS             complement(39866..40522)
                     /locus_tag="ACHARQ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8408798.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            40748..42682
                     /locus_tag="ACHARQ_000344"
     CDS             40748..42682
                     /locus_tag="ACHARQ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8408799.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(42768..43991)
                     /locus_tag="ACHARQ_000345"
     CDS             complement(42768..43991)
                     /locus_tag="ACHARQ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8408800.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(43985..45856)
                     /locus_tag="ACHARQ_000346"
     CDS             complement(43985..45856)
                     /locus_tag="ACHARQ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8408801.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(45877..46995)
                     /locus_tag="ACHARQ_000347"
     CDS             complement(45877..46995)
                     /locus_tag="ACHARQ_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8408802.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(47078..47911)
                     /locus_tag="ACHARQ_000348"
     CDS             complement(47078..47911)
                     /locus_tag="ACHARQ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8408803.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(47904..48821)
                     /locus_tag="ACHARQ_000349"
     CDS             complement(47904..48821)
                     /locus_tag="ACHARQ_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8408804.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(49048..50172)
                     /locus_tag="ACHARQ_000350"
     CDS             complement(49048..50172)
                     /locus_tag="ACHARQ_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8408805.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        14936 a        10143 c         8482 g        16642 t
ORIGIN      
        1 accgtgatat ttctcacagt cttttatcta ctctattgct atttgctctc ttagctagta
       61 ctttaaaagc taactcattg aggtagtgaa aacgagaggt gtattcagac agaaatccaa
      121 aatcctcaac tgctagctgt ggaatcgtag ttttgtgttt cacgatatcc gcaagcaggt
      181 tcaaatctga taataagaat tcttggctta tcagctgtcc gccaattatt tctaatgtat
      241 ccggatggta gatcagcttt gcatttactg ttttgttttc ttcgtcgtat tgtggaaata
      301 gatcaaattc tcctgaacac gaaccggtat tttgttccaa gaatggcgct tcatcttctg
      361 tcagccctac agttcctaaa aagtagtcta taatgactgc tgctgttatc ttttgtctga
      421 ggtcgtaagc ggttggctga ccactcaaag ttagcgctac tgctcttgcc atatgtacag
      481 ctcgagtaac taaaggtaaa aagagatgcc gtttattttt gtcaaaactg actgggacga
      541 tatctccaat cgcatagatg tccggatcac ttgtctgcag atattcattg actatcacag
      601 tctcatcctc atacaaagcc actttttctt ttaacaatgt cgtattagga cgagtttggg
      661 cagaaaaaat gaccatatct gcctttattg tttgagacag aagttgtacg gatactactt
      721 tctcttctga ttttttccaa tcgatcaaga attcatttgg ataaaaattt atttgcttag
      781 tttgcagctg attttgaaaa ctgttgatca tatccttatc gaaatatctt gataaaacat
      841 tcggcatata atcaactaga tgaataatgt atcctttatt tctcaaagca tctgctaatt
      901 ccaatccaat gtaaccagca ccgaagatca ctacttcttt tgcttctttt aaagcggcaa
      961 gtacttgctt agattgcact agatttttat aggtgtagac attttcagct tcaatttgtt
     1021 tatacaaggt gacaggagag gaacctgtcg ctacgattaa tttatcatag gttatggtct
     1081 cgttttttcc ttctttggat gcgaggatga gctgatgttt gtctggatca agttcagtta
     1141 ctctccattc ggtgatcagt tttgccccac tttcttgaag agtccgcatg ttatgggcaa
     1201 cttcagctaa actacgtatc tttcctttta aataaaggtt gattccattt gaaacatagc
     1261 ctagtccatc tttatgatct tcatcgatca gtaaaacttc tgctttcggt tgaagttttc
     1321 ttagatttag ggcagctgtg atgccttcat gggaagcacc aatgatcacg attttcatgt
     1381 tttattcatc cttaaattca atttttctaa ataacattct tttaaaaatt ggttcaaata
     1441 ggggaaatca taattatatt cgttgctagt gaatacaaaa actttagcgg acttctgtga
     1501 cgggtactct ttcaattggc tagtcaaaat gatatcatac tgcttttctt tctcgtattc
     1561 gactaaatca tatttgatcg tattcagcaa ttgttcctgg atgatttctt tcacgatatc
     1621 cgccattagc ctttcataag gaagatcata agcaatttgc agacgtaatt ctaactgctt
     1681 gatcagcgtc cgtaaaatag atgcatagcg agcaaataat tcaaagcggg cattttcctc
     1741 gtctacttca gtaaaatcaa caatagcaaa tgtttgtctg accaatcttt cacataaaac
     1801 caggaattgt ttatcttcta cttcagttaa ccgctgtttt aatccttctt ggccaaaaat
     1861 attcatccat cctctaaaat aggcaaaatg agcgtgattt tgcatcactg tcaacgcaat
     1921 cttttgcgaa atctcttctg gaaaaacata aaaagagaaa tattcttcta tcgattgttt
     1981 aaacgttcga ttcaattgag ctactacgtc gtctttgtat ttttcgctta tcaaaaactc
     2041 ctggacatgc gagttatgtg tatctaatgt aaagttactg atcatgaaaa gataaaaaac
     2101 caacgtttca ttttgattcc aaggaaaggc atattgaaag aagtaacggg tcaaaaatgt
     2161 atggaggtcc aataaaaatt gtgtcgtttg aaacaatcca acaggaagat tgctataatc
     2221 tggtttggat tttttcgtac agcgcattcg cttcttcata atggaaagcc atatttcaaa
     2281 tttcaatcgt ccttcttctg taatagcaaa actaaactct ttttgcagca gatctaaaaa
     2341 agtgtttcct gataaatcac tgtattcttt tttgttttca agatagggac gtccaaacca
     2401 aaataattgg aaataaaaat aacgaatttg actttctgga ccggtcagcc gcccgctttt
     2461 tattttcaga tgaaactcat ttaaagaatc atttaattcc ttcactctgc gagacaaagt
     2521 agccgaactg atattaagtt ccatgacaag tttctgccga tcaaatgagc cattttccaa
     2581 cagataaaga agtatctgat attttgtaga atagcgcaag taacaatgat aataaacatc
     2641 gactgctgct atatttccat gtctgattaa taaaatattc ccttcttgga taacaatttc
     2701 caattgtttg atattttcca agttagcttg taaataaaac agatcttctt tcaaggtagc
     2761 ccgatcaact gataagcggg taagtacgtt attaacggaa acctctcctt tttcctctaa
     2821 taaaagttgg aaaagtttga tttgccgttg ttcatttttt tcaagcaaac tgaaaagttc
     2881 caagtccact ccttcaagca tttctgattt taacagcaga agtttccttc tcatccttga
     2941 aaaagaggat ctgtgacagt tatcacagat ccatacaaat tctaatcact tagctggttg
     3001 gcccagctgt cagttcccat gtgaccatgc cttgatacgt tgcgccaggt tgagcattcg
     3061 tactgctatt tacctctaag agtaaaccat cggtatcggg aaagtcaacg agaacctctc
     3121 ctttagctgt tccatcactc tcataaatca acgtgttttg accttgctga atcaattgct
     3181 ttgatccaga atgattgaag tataattttg ttccgcttaa ttcttgacct tgatcattct
     3241 taaataaaga agtcaatgta cctgtcagtt gccaaggaga cgtgcttgga agccgagtat
     3301 cccgcacatc aaaagaaaaa tcgccttttg attcataata acttgtcttc aacggtttgg
     3361 ggagatcgtt accaaatgta aaatcatctg gtacattaac taatgttaat tcttgtttgg
     3421 ggacaagaat tgttttagca gatgtttgga caactgggtt aggaagctta gcaccatctg
     3481 ctaatgtaat attgctggaa agtacagctg cctttgtcag tatgctgacg gattgtaaag
     3541 aagtatctac agtatttaat tgatagttgt aagtaagatg aatggtatct cctattttgc
     3601 tcaatagatc tgctgggaca gaaactcctt gttgaatgcc ggtatctgcg atagttgtac
     3661 catttaattt taaggaacca ttgatataag tagtattttc aggaagtgag atcgtgattt
     3721 ttttgtcggt tatcgcatcc ccagattttg ttaacgtgaa tgtcacattt tcttgaatcg
     3781 tatctcctga tagagcagac aggggatctc ctgtctcagg ttgtctgcta aggtttttta
     3841 gctgactatc tgcactcaca gatggaaaat tagccactgt tatttgagga agagtggtga
     3901 ttccagcttg ttcagactgc catttgtacg caaaagacgg atacatgtta gtaagtacat
     3961 cgtccaccaa gcgataatca aaataatatt gccctgaagc caatgcaggt agtgtagaag
     4021 tagctgtatt tacacccccg ttatttgatt ggatcgtttg gaaaagttga tctgtccatc
     4081 ctgtcgctaa tttttgggtt cctttcgaat aaagcctgta atggatcgaa ccctttttta
     4141 gttcagttgc tgaccaattg gccgtgactt ggtacgatgc agaagaaagg agctttcccc
     4201 actgactatc ggctgttaag tttgttggta aaaccgttcc gtctagcttg tactgctcca
     4261 atacctttaa agcaacagga gaaagcaatc cgccttgcgc atctttccca ctgtacacga
     4321 tattggctgt ctgattcaat ccacttgtgc ttgctggagt atgagtcagt attgctcctt
     4381 gtgtaacgtt ttttcctgta aaatagttga ctacgcgatc aaagcgacta aatggcaact
     4441 gatcaaaacg tccttcagtc agccccatcg tcaatgtccg actattggta gagcctggaa
     4501 ggttctggtt atttccatcc acgtattctg tggtatcagc aaatgtatta tattttcctg
     4561 ttgaatcact tggtgagatg taactattcc acgtatttat ctctgcactt gcagaaaatg
     4621 catatgctcc tggattcaag tttccgccag ttaatttagg ataaaatcta cctgcagctg
     4681 gagctaaaat cgaattgaac atacgatctt ggataatatt attcgaatca taaaaatcaa
     4741 cagaaaaaac ggccttttta gacatagaag gttgtaattt acccttggtc aaaattttgt
     4801 ttggactcgt atcatattta tctccgtttt ctgtcatctt cgcgacatta gctgtaacat
     4861 caaattgaag cgggatatcg tccacgttga attcgcctcg cgtaataata gcaataatat
     4921 ctgtcaatag agtatttgta tcccacgacc ccatcccaaa acgcaaggta tggcttcctg
     4981 ctgataataa acttccgcta gtaaccttga tgggggcacc ggcaatatac aagtaggcgt
     5041 tgttccagtc aattgctttt aatacatcac tgcttgatac atgttccggc aaattgatga
     5101 tcgccacaaa tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca tttgttagtt
     5161 tcacatggct ttcccaatga aaacggaccg tgccaccttt ccaaggatca tttgaaccat
     5221 cgttcaaata agtagcagtc aatttaggca attggttagc cccagttagt tggacaagct
     5281 tcccgccatt gactgcacta cgtccaccat ttggaggtac gatattttca tacgtgtatg
     5341 gatcataaga aatggcagga ggcgttgctc caacagcttc tgccgaaggt gaaaagataa
     5401 atatacttaa aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa aatttatttt
     5461 gtagcaacat gttcatcctc cttttccttt cttctccata acaacaacca cgcaagaagc
     5521 cataattcta taccaatgat caaataaaga aaacggatct tttgttcatt cgtctccggg
     5581 aatacgccat gttgattaga ttctttataa atcggtagct gtgtctgctg ttttgatgcg
     5641 ccattgactg aaccacttgg atgcggaatt ggacttagat cataaaatgt aatcgtacct
     5701 gaacttttgg tatctgcttg gactggctct tgccagctga agaacagcaa acttgcaata
     5761 atccctccca ataagatcga tgttttcaac gaaatcacct catcatgttg tttctccttt
     5821 tttctttttc ttgtggaccc acaaaaacag caaaatcaac cataacacta gaactgcagc
     5881 aaccaatcct gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa cggaattttc
     5941 atttactttt tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc gccattcatt
     6001 actgtcgcct gattttgcta tgatttctgc gcggtaatcg ccggcttttg ctttatcacc
     6061 agctaaatca agattcaagt gaaaattcga atttggtgca actaaaaatg cattagacgt
     6121 attttcaata taaagctgat tttcataaaa gatcttgctc gtaaatatca aattgctgat
     6181 aatcctaggc tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt tgacctcgtc
     6241 caaatctcct gataaaagtt tcagatccgg ttctatcgtc tcttgacttt cttgtaaaac
     6301 gactgcgaga ttataagcaa tacgattttt gatttgtgct ccttctgtag cattttctgt
     6361 ttcagcttct tctttaggtg aaacatgaat acccgctaat acacgtccct taaagctttt
     6421 tgctgggatt ttcacttcta taggtacagt tgtttctgca tgtgctgata cattgatttc
     6481 ctttttcgca atgagcaaaa cgtcacggac atcaaacggc aatgtctcat ctaacgttgg
     6541 gtttttttga ctataatcga tcgttccacc atcactcgtt accgccggat tgacagagac
     6601 ctcaaatgtt cgttcttcat ctgaagaatt gctgataatt acttctagtg tctgattttg
     6661 ttccggtgcc actaataaat caaaataacc gattgcttta tctatttggt tctctggaaa
     6721 taccggtttg attccaaagt ctcctgctga atcctttgct tggctgattg acggaaaaat
     6781 gaaaaataga cttaaaatag aaaaaaacga aacaaacagt cgtttacttt gattcattgt
     6841 ccaccacatc ctttcaacaa aaactgcggt aaaaaatcct tcacctatag tattttcacc
     6901 gcagtttgtt gaatttattt catactctat gctactgggc cagccacgag gttccaagtc
     6961 aaatcagccg tgaatgttcc agctgctgct ttcggcactt tcagtgtgat atttgctgga
     7021 tcataaacag aaagccatgt tcccagacct tgatcttttg ctgctgtaaa tattggtgtc
     7081 gctgcacttg ctttactcaa cccagttaca gtttgactag ttggtccagt ggattcgtta
     7141 tctgacgtac ttttaacagt ggatgttcca aaatctaatt cagcgccttg caaaacttgt
     7201 gaaccgttta caaaatctga aaccgaaact gtcacgttcc acccttgtgc ttggccatct
     7261 gcacctacac ctcgacgatc tgatacttgg agataaggtg tatcgttttt tgaagcgtct
     7321 acttgatacg tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa ttcgggaact
     7381 acatccagtg ttaaaggacc tgtctggcca gttgctggat tttccggatc tgctggctct
     7441 actggttttg aaggatcagt agggttaaga ggctccacgg tatttgttgg ttcagtaaag
     7501 gtaatatctc cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc caagccgcct
     7561 agaagaagtg ttgaaagtaa taatgtccca atcatgttct ttttcataat aaatcactcc
     7621 tttttcttag tgacctaagt atagtagaga cagtatgcaa aaaatcctca tgaattcatc
     7681 cgcttacttg aaattatgac cctttttgta taaaagagaa aaaaacaata aaatcgttta
     7741 catgaatatt cttccttttt tattaagtat gaaaaaatat ttcaatttta ttttttcgtt
     7801 tcaaattatt ctattagtga catccttgaa ctataaaaat aggagtacgg catcatctgc
     7861 cgcactccta tttttttcgt catttgtttc ttaaataaaa cgattctcac gcaattttat
     7921 ttttttgata tcttctactg tttgtgttcc agctaattgc atgaccattt ccaattctgt
     7981 tttaaagaaa tcaaacactt gatggactcc tgtacttcct cctaaagaca atccataaat
     8041 aacgggtctg ccaatagcaa caagatctgc acctgatgcg attgctttga aaacatgctg
     8101 tccgcgtcgt acaccgctgt caaacacgat tggtacacgt ttatctactg cttctgccac
     8161 atattgcaac gaatcaaacg ctgctggacc accatctagt tgacggccac catgattaga
     8221 tacccagatt ccgccagcac cagattctaa tgaacgatag acatcttctt ctgactggac
     8281 acctttcaca tagactggca aatcagaatg agcagcaata aattctacat cttttggact
     8341 aagcttctgt ttggaagatt tgtacacagc atccatcgtt tggcctactc ctgattgata
     8401 agcctgaacg atcggcattg gcagtgggaa ggtaaaccca tttctgcggt ctgtttcacg
     8461 gtttccgcca actgttgcat ccgctgtcaa tacgattgct tttgctccat tacgcttggc
     8521 cacttcaaga atatccaaat tgatcccatc atctttgctc atataaaatt gaaaccattg
     8581 cggtgctttc tccccgcctg cttctcttat ctcttctaat gtacaagaag cgtaagaact
     8641 tgctgtatag atcgtcccaa aatctgctac accttttgca gaagctttct ccgccttcac
     8701 atgagctaag ccatgagcag caactggtgc catgatgatt ggtgcagtca gcgtttcttt
     8761 gtcaaaatgt gtagttgtat cgggtaattc tacatctttt aatacatgag gaataatcag
     8821 ctgatgattg aatgcccgtt cattttcttg ataagtaaat aagtcgccag caccactgct
     8881 gatgtaacca taaccaccag ctggaatcac ttgtgaagcg gctatttcta aatcttccat
     8941 attgataaaa tccactattc cttcatttgt acctgcttga tacgtttttt ccatttcaat
     9001 gccttccctc tataaaaaat tgaatgaata atatgatagc gtttacacac tttatattac
     9061 tcttccgtat tgatacaggc aaataatctg tttacatttc agtcattcca gttgttacta
     9121 ctctgaaaga aatgtttaga acacaaatag ataaaaaacg atgggacttt gacaaaagtc
     9181 gtgtcaaagt cccttttacg aaatactgtg gatgcctatt gaaataagtg cgggttgttt
     9241 ctttgtattt caatcgtttc ttgccattct tcgtttaatt ttggattacc gttttcttcc
     9301 ttgaacgcat aaggtcccaa tccaaaaagc tcagttagta tttgattcac gattccatat
     9361 agctcggtgt gatcggacac atgattattg aagatcatct cattatcacc acgtttgaat
     9421 ctcacattcg ggctctcatg atgaacctct atgcagtttt cccctttttt taactgacgg
     9481 atttctgaaa acatctcata aatatcttct ccaataaaac cttcagcaag cgccagactg
     9541 tccacaagac cgcctactgt caactcagag attatcgctt tgttggcacc aagctttaat
     9601 gtatagcttg ttgcttctac tacgccgcat gcattgcaac caccttctga tagttcgctt
     9661 tttctgatca attgaacttc tttcattttc tgttcctcct cttagttttc tacttatgta
     9721 ttttttatca cctgacgtaa gtgtatttta tcgtcacgat cacgtgattt attcttcaga
     9781 gattctattt atctatctaa catatccttt attcttctac ttttctttct ttagcatata
     9841 agagatacgc atagtaatca agcaaggatt gttgatataa aaagaacaaa taccgctatt
     9901 ttgatttctt ttgactctat tactcatata aataaaattt attatacctg ctctttttga
     9961 ctatatcctc atctgactcc tatacttcgt ttgtattaaa acttaggagg aatcattcat
    10021 gcttacaggg ctaaacttat taaataatcc ttttctaaat aaaggaacag cttttacaaa
    10081 agaagaacgg gcaaaatacg gaatcactgg tatgcttcca agtactgtcc aaacattgga
    10141 acaacaatct gtccaagctt acggtcaata cttaagcaaa cagaccgatt tggaaaaacg
    10201 tatcttctta atgaatctat tcaatacgaa ccggacattg ttttataaat tgatgggcga
    10261 acatttagtt gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt caatcgaaca
    10321 atacaacgaa attttcttga aaccacaaga tgctgcattt ctttcaatcg acgaaccaga
    10381 aaatatcaaa gcaagtttga aaaacgcagc agatggacgc gacattcgtt tgatcgttgt
    10441 gacagatgca gaaggaatct taggtatggg tgactggggt gtgaacggtg ttgacatcgc
    10501 tatcggtaaa ttgatggtct acactgctgc ggctggtatc aatcctgcac aagtcttgcc
    10561 tgtatcaatc gacgctggta ctaataacga agaattattg aataatccat tatacttagg
    10621 gaatcgtcat gctcgtgtag aaggcgaaac ttactacgaa ttcattgatc aattcgttca
    10681 atcagctact gaattattcc cagaattgtt attacattgg gaagattttg gacgaggaaa
    10741 tgctgcaacg atcttagaaa aatacgaaga taaaatcact actttcaatg atgatatcca
    10801 aggcacagga atcgttgtac tagctggtgt attaggtgga ttgaatatat caggtgagca
    10861 attgaaagat caaacgatct tgacatttgg tgctggtact gctggtgtag gtatcgcaaa
    10921 catcctttta gatgaaatga tccgtcaagg tgtacctgaa gaagaagcac gccaacattt
    10981 ctatcaagta gataaacagg gtttattgtt tgaagataca aaaggattga ctcctggtca
    11041 aatcccattc gctcgtaaac gctcagaatt cactaatgct gatgaattga caaacttaga
    11101 agcagttgta aaagcaatcc atccaacgat catgatcggt acttctaccc aacctggagc
    11161 attcactgaa gaaatcgtga aagaaatggc ggctcatacc ccacgtccaa tcatcatgcc
    11221 tttgtctaat ccaacaaaat tggcagaagc aaaagcaaaa gatttgattg aatggacgga
    11281 cggaaaagct ttagttggaa caggtatccc tgctgctgat gtggaatata acggtgtaac
    11341 ttaccaaatc ggtcaagcaa ataatgcgtt gatgtaccca ggtttagggc ttggtttgat
    11401 tgcttcaaca gctacacgag taaatgctga aatcatttct caagctagtc gagcattagg
    11461 cggaatcgtt gatgtaacaa aaccaggagc agctatcttg cctccagtag caaaaatcac
    11521 tgaattctct caaacgattg ctgaaactgt cgcaaaatca gttgttgcac aaaaattgaa
    11581 tcgtgaagaa atcacggata tcaaagaagc tgttgaatct gctaaatggg ttcctgaata
    11641 taaatcttta gaagattaag attgttatag taaaagaaca tacagaaact ttgtatgacg
    11701 tctaaaaaga aaaaagcgac tgcggcaaaa cagcagccag ccaactactg ctcttttgcc
    11761 gcttctcttt ggcttttttc atacgataca gaataagaaa ggatctgtaa aaatggcaca
    11821 aaaaacaaca accgcaactg cagttgagga aaagaaaaaa gaagggtttt ggtcaacaaa
    11881 aattaccggc gtaagtttgc ccctttattt gatcatggtc cttgtcctga tcattgcaat
    11941 ggctttagga aaattaccga cgaatatgat tggcccaatc gctgttttgg tcattttcgg
    12001 taatttattt cattttatcg gaacaaaaat ccctatcgtc aaaagttatt taggtggtgg
    12061 ctccgttttt gcgatctttg cctctgccgc gattgctaca tttgggatct tacctgagta
    12121 tgtagtaaaa tccacagaaa actttgtcag caatatgggc tttttagact tttatattgc
    12181 tgccttgatt actggtagca tccttggtat gaaccgtaat ctattgatga aagcttctgt
    12241 tcgttttatt cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg taggactcgt
    12301 aggtatgctt atcggaaatg gttttgcaaa ttccgtttta tatatctcat tgcccgcaat
    12361 ggctggtggt gttggagccg gagctgtacc tttatcaacg atttatgcaa atgttttagg
    12421 tacggacgct tcttctatca tttcccgcct gattcctgcg acagcaatga caaatgtcct
    12481 agctatcatt ggtgcagcgt tagttgctcg tgcgggacaa tcaatgccaa aattcaacgg
    12541 taatggtaaa ttaatggaaa aaggcgacct tggcgatgga aaacctagaa atgtaaaacc
    12601 ttccattcca caattaggtg tcggtttgat ggtatcattg actttcttca tcttaggaca
    12661 aatctgtaac tattttgttc ctaaagtcca tgcttacgca ttcatgatca ttatcgttgt
    12721 tatctgtaaa atcgcaaacc tattaccaga atattacgaa gatgcagcta tcatgttcaa
    12781 caacttgatt gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta ttgcgttatt
    12841 gaatctaaat gtattggctt ctgctttgac atggcaattt gttgtcttat gtttgacaag
    12901 tgttatcgcg atttcagtag tttcaggatt tgtcggtcgt ttgtttggtt tatatccaat
    12961 cgaatcaact attactgctg gcttatgcaa caactcgatg ggtggtacag gaaatgtagc
    13021 tgtcttatct gcagctaatc ggatggaact tattgctttt gctcaaatgg gcaatcggct
    13081 tggtggtgcc atcgttttga tcatctcagg tttcttgatg caattacttt cttagtcaaa
    13141 aaaaatacct gatcatcaag tatcggcttg atgaccaggt attttttgat aaaaatttac
    13201 aggattttca aaaatctttt ttgcatttta tcggaatgat aaccgatgcg ttcataaaat
    13261 cggtgtgctt ctactctgta ttctgcagaa ttaagtctta ttgctgatat tcctcgtctt
    13321 ttcgcttctt ctacgccagt catcaaggcc tttccgattc ctcttttttc agcatgttta
    13381 gacacagcaa gtgccataat attgaacatc ggatcgctga aaatcgattc ataggactcc
    13441 gcttgtacat agccagctgc ttttttagaa gtaggatctt catagaccaa gaaaaaattt
    13501 ttttctggct ccttcaacag tttcttcatt tgtctcaccg taacggttaa tggtacatca
    13561 tatcctaatt gttccgcatt gatttctcgt agtgcttctg catctcctaa acgcatttcc
    13621 cgcaacatcc aaatcaccag ccaattttta tttttttaaa tgaatttttt tatcacagta
    13681 tactcctttt ttcatttaga acaatatgtt tcagtgattt tgacttgcgt tagtctagat
    13741 tctcctaatg tttgtaagat gacgaaagca ttgtaaagat tgatcaaaag aaccacgagg
    13801 acaattactt gaaaaataaa ggtcactttt tttggagaca aaaccctgct aaatcttgct
    13861 ccccacaaac ctccaataac agcagcgggg ataacataaa acaacataga taaatcaaag
    13921 cgcataaagc tacttgttaa agcaatcgtt accaattttg ccagttgaga aaagaaaatc
    13981 gtagatagag aatatagtgt tgcttctttt attggcaatg caaacatcag catcaacaaa
    14041 gaaacattga ctggtccacc accaatacct aaaaaactag ctaaaaagcc cagaaccccc
    14101 ccacatataa ggtaccaagt ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg
    14161 tagaagaacg caaaaaccaa ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc
    14221 ttctcgtttt caaataactg taaaaaaact tcgaatacaa tgttgccgac tactcctcca
    14281 acaacagctc cactagatac ccaaccgaca atctgccaat tcagtgattt cccgcttgat
    14341 acttgtcgca cagtcgaaac aatggacatc gtgaataccg caactgttga gtaaaaagaa
    14401 atccctgcaa cagaatgggc gccgataaga tcaagaatcg gtttgatcaa cacgcctcca
    14461 cccatcccgg aaatagcgcc aactgtgttc gccagaacga tcaccacaaa ataaatcaga
    14521 ccaatcatct tgtatttcct ttctcttgtt tatatctttt agcgattgtt actcttttct
    14581 tttattatac agtaaaagaa acaaacgagg cttcgaaagc ttttctactt tttaaggaaa
    14641 tttagtgcta aacgaaaaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag
    14701 ccaaacaaaa atagctcctc ataattttcg tttggttgaa cttattaccg cagatgcaag
    14761 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg ttcagctccg agtcacaagg
    14821 aacaatttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
    14881 tggcaggaga acaccgttta aggaagagac tgtgaagtca acgttttgac ctgattatta
    14941 gagaaacaaa tggcaaaata gttcttcata tttcgccgtt tttcgagcga atgccgaggg
    15001 gcagctgatt gaacatcgtt tatttggaaa aagggattgt gacaagacgt ttgtcacaat
    15061 cccttttttt atatcttatt aattcattct tttgttctat tcttcttctt ccgccatgaa
    15121 tgagaaaccc atatcaaaga actataaagg ttatcaaaac agcgtttatg actaaatgaa
    15181 ttagagttat ctatcactat ttttcaagcg atttaaacca aatggcatac aatatggcat
    15241 acaaaaactt caataagccg tgggggtttt taaatttctt cttgttcttt aaaatcaatg
    15301 agtggagcta cataatttga atccactttt tcaaacgtca ctttttgcat cgacgaatgt
    15361 tttctttccc aagataacgt aaatagattt gactctggac ttataaagtc tacttcacct
    15421 tctagtatta ctttcttatg caaatattcc tctaataaat atagacttgc taaagcatac
    15481 aacacatttt ccaaatttgc ttccttataa tttgaagaac gaccatgttt tactctgtta
    15541 tatattttcc accaatctgg tgtatcttca ggccacccat ctccaaaagg ttttagtgaa
    15601 gtcaatgtta aagaattcat aaccctaaca ccattatcca aacacattaa ttcgctagat
    15661 ttaataacag ctttaaaatc agttatgctt ttattgctac tgctatcaaa tccgcaaagt
    15721 tctttgaaaa taatctcaca ctccgaacca atcgctagta gtagtttttg atattcaata
    15781 gagaatgttt tgaaattatt ctcatcgagc atcacaaaat ctgtggtatt aataaactca
    15841 ttttctaaat gtagataata cttccaataa aaatttgtaa actctgcttt gtccattaaa
    15901 tcacctcttt tgaattataa caaaaaaaac acgtccacaa cagacgtgtt ctttgatttc
    15961 tacacaatca gcttgccaaa accatccaat cagtcctagc atgcctgtat taaaattgaa
    16021 cgttcatcat ctatcatctt tcttagtcca gcatgtttgc aaccacacct acaattttca
    16081 gtccaacaat agatttcgac tagctaagtc ttgcaaaatt caggcagcta acccttcatt
    16141 tactacctta attatactaa atacccacaa cgtttacaag tgtaatcatt ctaagaaatc
    16201 tgtttagcta tgatcgttgt ataatcttta aacgccgttc cttcattgat tgaaataatt
    16261 ttataactct tgtttttcca agctactttc atatcgtttg ttagttcata atcttgatcg
    16321 tatcgaataa taaacgtcaa cgttccttca agaatggtac ctacactagc aagcacgtct
    16381 tttagcattt gtgaacgcac attagcccaa cattcaaata cagtttcttc actatccacc
    16441 atctgcccat cttcatcttt tacccgtttt gtctttttaa acttgactct ctcagtcaat
    16501 tcatttacat tcttaatgat tggcataata agcacctctc atttgttgta ctaatgcttg
    16561 taccgttact ggtatccgtt cgctgctggc ttcttgacgg ttcaaatacc aatgttgagt
    16621 aagtaatgac accgccaaat caaattgctg ataattatcc atatctttat ccgttgcgct
    16681 actatcaata gcacttttta tatattcgcc tgctgtatca atcaattgtt gaataaagtc
    16741 atcatcaata gtgtgatcga tccgcatact tttttttatg ctatccaaaa ttaccatttt
    16801 atctctccta atgaaaaaaa ggcagtggga taaaccgccg cccaatattt ttctatactt
    16861 ttggtgttac aactggcgta aactcaatat acactgaagc attttgatca attttcttat
    16921 aatcgttgcg aacgatcact gcaagtcctt gtgagtagta atcgaatttt tcccattgag
    16981 tcgttacttg atttctacga gcaacaaaga tagattgtgc aatatccccc ataatcatag
    17041 ggaatgttcc agctttagga ttggcaaaca atgtatctgc aatcaatact actggcatac
    17101 cgaataatga tttaccacta ggtgctgtca catcgggttg taaaatgtaa cgtccatctg
    17161 aatctttcaa tgtatctagg tggtaatatc cgctttggtt tagaattacc attttattta
    17221 atgctgggtc taatgccaca ttgtatagtt gtttcaaatc gtccaacgta gcagccgttt
    17281 tcttagtgaa tgtttttaat aaatccatga tatgcttatt gtcagtgttt tctaccaatt
    17341 tcgctaactg atctttgacc tcttgtacaa tattcactgc tgaatcttcc acaacctcat
    17401 ttgataaggc aatcttacca gcacgagttt ctactttata atcaactgaa gtaaacattt
    17461 cagcgtcaat atcaccaatt tcagcaagtt cagcttttgt tgctaacact gcttgttggt
    17521 tagttgctac tggatattta ccttgtccat ttgatactgt ttttactgtt gcatattgag
    17581 ctaagttata atttgaacgt ttcaaatcaa agacttcacc gattacttct tcgggaacaa
    17641 ctgccgctgc atttacagta gtgactccat cacgcacttc gccttgtgaa cggatatatt
    17701 cttcataccc acgcaattct gttttttcgt tatcgattaa agttttttcc atgttattac
    17761 tctccttttg tgctttagaa gtggattcta agctattgtt tgtttgtttt gcttggttgt
    17821 tactcataaa cgattcgtaa gaacgcttat ttacttggac atttgatgaa tcataagcgg
    17881 gaacggtcac gacactaatt tcattaagtg ctttgatttt atctattgat cgttcaattg
    17941 tgccatcttc ttttttgtcg aatgaatcta atccaagcac gaacccaaag gacatagaat
    18001 caacaacccc ttttgagata ttctcataca catcatttga ataggtcgtg tcggttagct
    18061 ctgcttcaaa atgtaatcct gtatcatcga cattcaattt caacgtacct gctttgacac
    18121 tagctagtgg ctttgaataa tcgtgtccat ataataagaa cacatttgat aaatccacct
    18181 cttttaatgc ttcgggtgta atcacttcta caaagccgcc taaatctttt gacggttgcc
    18241 cgaatttcaa cgcatagcct gaaatagttt tgccgtctgt tggttgtttt tcattttctt
    18301 tctctacttt gggagaatcg gctgttagct cagcttcttc tgtcagccgt ttttcctttt
    18361 cttcttcatt attcaactgg tgtaactcct tcctgttctt ttaattgata tttctccaac
    18421 gtatctaaat acgtaaagtt caaacttgct agtaaacgat cgccaccatc catggggggt
    18481 agccccattt tcgaccgccc ctcattaatc gtgagaagtg accctttgac ctgatccaat
    18541 acatttttta cttttgtttc aggatcagtc tctagtaact gatcggaatt aaagcggata
    18601 ttggtttcaa gtttcgtatc taattcactc acaaacacat taaagtaatg aatcagcgta
    18661 ctttggatat actggagatt actttgaacg gtgctagaat gttcattttc aacgcctaaa
    18721 cgctcaatcg gtacaccaaa cgccttggca atttgttttg tgttccaatc attggaattg
    18781 ataagcttca aaacatcggt gtttatttct aatgttttgt agtccattgt ttcatcaaga
    18841 atgatcgttc taagagcatt atctccacta cttgaaccgt tggcttcttc aaacttttca
    18901 cgaattgcgt tctttgctga tccgtctaaa tctgacttat gaaccttcaa aatacctgac
    18961 ccactgacac cacgagtaaa gaaattgtgc aatgtgcggt tgccagcttg ttgaattttc
    19021 agttcatcac gtaacgcata aagtggtggt aatcctgtta aaccatcttg ggtgaaatat
    19081 ttgaagtgca aaatatcgct agacttcaca cgtctttttt tatcaccaat ctgataagac
    19141 aacgtaccat tatctaattg agtaaccgtt acacttgagt taggtaataa gtgaatttct
    19201 tctacctttt caccgttgcg tttaatctct gcaaagctat taccgtttag caacatattg
    19261 accgccaaag caaactttaa atgccaacca tccatttctg aattaggttt ctcgtttagt
    19321 aacttcacca gttcattatc agcttgtggc atattgtttt tgaccaattg aatcggactt
    19381 gaagcaatat cactagcaat aattcgaacg gctgcaaaca catcactatt tcttaatgca
    19441 cgaacgcttg taaagctggt ggtgtaatca tccgattgga ttgataccac atgttgtagg
    19501 aaaggatctc ccgttgtatt ttgtctcgct ttaaaaaatg ccatctttta acctcccttc
    19561 tgttctgtat ttagcatgac cgctacccca ataagcacta tgcccagcgc taacaagcca
    19621 tagtagacat ttgttagtaa ggtgatagca accaacacac acaccaatcc aagaagtaaa
    19681 agaacggtat gaatgtaatt agaaagaaaa ttcttcactt gtataaaatt cattgtctgc
    19741 tttcgctcct tccgttgtgg tgaagtaatc cattgcaaac acataggcat tgattaatgc
    19801 agctattgga tcaattttgt tactgttctt tgatttatta atctgaatgc cgttgttatc
    19861 cgtttttaaa atagcgttgt tcactgcatg ggtgagaatc gtatttttat tgtgaataac
    19921 gttgccttca taaacttgtt ctctaaaagt tcgggtcgga acgtttagag taatcgttcc
    19981 ttgtcttact tctaacattg ggtagttggc tttttcagct ttactgataa gcgaattagc
    20041 gttatacggg tcataacaaa tccccataca ttccaaatca ttttcttgaa tcaaatcttg
    20101 aataaactga aatacttggt catagtccac gattccactt tctaattgag taatggaaca
    20161 ctcaccagct ttttctagtt ctcgataagg caaaccgtcg cgcttttctt tgtcttgaag
    20221 cccatatttg gtggctacaa agctatgtga atcacaataa agttcgccgt tatctagcgg
    20281 tacgatccat gaaacgctag ttaaatcatc tgttttagat aaatccactc cgatataaac
    20341 gggcttgcct gtaatatctt gtggttctac ttcggttgcc tgccagtctt cagcaatcat
    20401 atagctgtcc tcactcgctt gtctccataa attgaagttt ttcaccaata cagaattaag
    20461 gttattttgt ttcaaagcaa ggttcacatc atcttgaatg gttggaatca ttactttttt
    20521 gatttcttca ctttcaaaaa tcggattggc tttgatccat ttctcttgat cgtgaatttc
    20581 ttcgggatcg tccaattccc atatcgctat aaagtacctg tcggcttgtt cttcgccttt
    20641 tagcacacga tctaacaata agtattcctc gtacatagga acgtttaaat caagcccagc
    20701 ggtactaata acacacaata agccgttctt ttgttgggtc attcctgact taatgacgtt
    20761 gtacgtttta cgagtttttg cttcgtgcca ctcgtcaaga attgcggttg ttggtgcaaa
    20821 cccatctagc gtgctggttt cactagccag tgccatagcg aacgaattag agggcaagtg
    20881 ctgaatctta gagttcataa ttttcagttg tggtcttaaa aacttactag acttgaccac
    20941 gtttctaagt gaattagaca gcatatcata gcccaattta gcttgtttca aagcgttact
    21001 tacaaacaat acttgccttg cttctgctgg ttcttgttcc ataatcaacg cattggcagc
    21061 catgccactt gcgatatacg ttttcccatt cttacggctc atactggtaa acgctcgatt
    21121 gaatcgcctg taattccctt ctttagtacg ccagccataa agactgccta gaatgaattt
    21181 ttgaaatcct aacatcttga ttgctttacc gtccgtagat ggtagcaact caatgaattt
    21241 aatcgctttg gtggcttgtt cttcatcgaa cacataaggg aagctatctg acttagaacg
    21301 ctccaaatcg cttatatgac gctcacacgc ttggataatt ttcttgggtg caatcagctt
    21361 acctgatagg actttctcaa tgtaatcatt catgaattca tcaactcttt gaacgggtct
    21421 tcgggttcat catttttaac cttgttcaaa gctagttttg atcgtgattc taaggtcata
    21481 cctagtgagg tggcttgttt tttcaaatca ctcatagctt gtgattgaac ccgaaccgct
    21541 ggattggctt tctttactcc gttttcgcat gtgataaacg tacctgattt tcttatctcg
    21601 gcttcggctg ttttcattcg agcaaaggct aaacaatagg ctaccaatga accataatct
    21661 gcttcactca atggataatc tttttttaat gttggtacga tacgattcca ttcacttcta
    21721 gcgctcgctg ccatccagtc agggggcgtg atatttatta atggttgctg cttgaacaat
    21781 tcttctctag cgtccacacg gattgctttt tcttcacttg atatattgcc ttttgtatcg
    21841 tgtaatattt tagggtgtcc catgttgtcc tcctttcaga caaaagaaaa aggaacgtaa
    21901 gcaattaagc tcacattcct tcgtttttcg atcagaattt ttctttatct aatttatcta
    21961 tataaatgat ttttccgttc tgcatttgaa tggtcacttg accaaaatga ggtaactcaa
    22021 tggtgcgctg tttccctgtt ttatcagtaa taacaattgt atttttcaat aaatctccta
    22081 ctttcattat atactttttc acttgtgatt acaagtgtaa tcgttatttg taattaccct
    22141 tatgcctttt cacaacggtt atttgtacca aaatgagtag gcatcgattg tcagtaagtt
    22201 tcacactatg cccccttaac tcaaagggta ctcttttctc gtctttgtct ttcattggct
    22261 gtcttggaat tatggtgtgc atggcataaa gaacgaaaat tagtttcatc taaccgtcta
    22321 ttccaatcat cttttagttc aattttgtga tctacaacat cagctttcct tatcaagcct
    22381 tcttctaggc aatcctcaca tactggatga tttaaccgat acaagtatga agcctttcgc
    22441 catgttctac tttgatagaa ttgaaaatat ttaccttcgc tttgctttct ttcttcacgc
    22501 ttaattgccg tttgctttgc tttatgcttc ccacaatact tctgcctata atcaatcaat
    22561 gttttacatc cagcatgatt acattgtttt ttaactgcca tagattacca accaaagcga
    22621 tctcgttcgt cagaatcatc ttgttcccat tttgatttgt attcaatata aggcgaaact
    22681 atcttcaagc acgttccgtc tttaaaatta actttagtga ttttatcttc ctcaaaacta
    22741 ataaagtcta catcttcttg agtgtttgaa tactcaatgt gtttctcacc atcgaagtat
    22801 ctaatacttt ccatatgcat aattattcta cctccaataa attagttatc aattcatcat
    22861 ctgttagtag caaagccaag aacttatcat ggatgtttaa aaacaaatta tcctcattcg
    22921 atgtaagctc tcgttgtctc gctcgatcat ataggttaag cagacaatta aattcatcca
    22981 ttagctcacc tgtatcatca tagttgccac tatctaccaa tgatcccact actagcgatt
    23041 gacaccacgc tactgactcc tgcagccaat caggttcttc ttccattatc tttaatactt
    23101 ctggatcaaa tgtggaataa tcttctgtta ccaatcccac actaaatgcc agcgtgttca
    23161 ttagaaaccg atccatttca tctgtatatt gaaagttcgg tgctttgttt gttgaaataa
    23221 tattgagcat ttactttcct ccttgattcg taacacgtgg taacacctac gtaacaggta
    23281 cccgttacca ctcaatccca acggggacaa gggttctagc gttttggtaa caggtaacgg
    23341 gtaaaatcca gtaacattat tccccagcat attagctaac tatttcaaaa gttttaagga
    23401 aataaggtgt taccccgtta ccaaattgct gtatcccttg gggcagtaag ggtaaactag
    23461 taacgggcta ggtgttactg acccgttact acccgttact ttttgtactt tccagctcga
    23521 tttttatttt cataggcatt tgacttattg taaaatcctc gtcgtttatt tctttttggc
    23581 agttgcttcc gttcccaacc aggaatactg ctaataatca aggcaatctt tttagtttct
    23641 gcatctgatc gaccattcaa taaatccttt gcttgtttat caaaaacagc ttccaaaata
    23701 tcagcggtga gtacgctatc aactaaataa aactcttgtt gcatagtagc tccattatag
    23761 aaatgctgta aatttttctc attacattta ctgtcaggat agttctgata ataagaacgt
    23821 ctcgcccacg ctggagcttc ttcccattta tacgggattt ccatttccaa atatttagtg
    23881 attgcttctt taattggatt ctccatcttg gcatcctctt gatagtcttt tgcaatttct
    23941 aagacttctt tatcttcatc gggactaaaa tagattcgtt gtcctttatc aaaaagcacc
    24001 tttgcttcag ctaacacttg aagaaaatat tcatcctttg ttttaaacgg attcatcttt
    24061 ggcttattct tacaaggcaa aggataaaaa cggcggtttc ctgtatcatc tttcaaatac
    24121 tgcccatcat tcgacgttcc aataaataca cattgccttg cccaagctat gacattccga
    24181 ccataaggag gtctaatttt atcgatcttg gcactgatga agttttttat ctgttcaatt
    24241 ttagttttgt tcattgaaga taattctcca agttcaataa ttacattgcc ttgaagctgc
    24301 atataatcat ctttcttttc acctagcgaa tctaatgaat cggtgaaaaa atcatccgtg
    24361 taaagtaatg ctggaacggt actcttacca atcccttgcg gtccatccaa aactggaacg
    24421 atctcaaact ttattcctgg tttatagata cgtgcgactg atccgactat ccaacgtttt
    24481 gttacttctc gtgtgtaaag gttgtcgtct acccctaaaa ggtcaataaa cagtgtttcc
    24541 agtctaggct gttgatccca tttaactttt tcgatacgtt cttttactgg gttgaaaaca
    24601 ttctttctag ccacatttac tactgctttg tgtattttgt tgtcatcaaa aactacattg
    24661 tagttttctt caatatatgc cgttaaggca tcctcaaaac tgtcttccca ttctccagcg
    24721 gtacgattga tccaaggaga atcttttaat aggtgaatgt atccgctaaa ttcgttataa
    24781 gcaattgttc cagctaaatt gctatcattg gttaaaatca attctgcatt cacaacactg
    24841 ttacttttaa tctgatcgtc tttattgcat cttaatcgtg acatccaggt tttcaattca
    24901 atgatattgt cttcacgctc gctggcttcg ggaaagtcta aaatactatc taatttatgc
    24961 ttatgagcca tgagctttcc ccctcaaatc ttttttaatc atcgatcgaa taatttgatt
    25021 cacttcttta tcttttagcg gtggttcatt gtacgcattt gctgtataaa tcaattctgc
    25081 taaagtttga ttatctacac cgtgccatag taaccaaccg cataacttag ttaaatgatc
    25141 gtttctacta ccttcgccag cgccttgaac aatcatatct aatttttttg ctgtccctgt
    25201 ttttgctacg ggtcggtatt gagtctttat ttcttgcggt ttttttagca gtaggttcac
    25261 tagccaatcg ggagctggtg caatattatt tagagatacg ttttcaatag gttcatatgc
    25321 tctcccaaca cttcttgaaa taatcatagt tggcgctatt ggaactcctt tagtgataat
    25381 atcaatcccg cttaaacttt tacctttatc atcacgcccg aagatatcag caaatgcacc
    25441 tgtttcttgt tggataatta caccatcagg aattttaaag aagaaatgga ttcctttacc
    25501 tggtgtgatt tccgtataag tagaaggaaa tggatcatac attgaagcaa gttttttata
    25561 attctcaacg ccgtttcgtg tttcagaatg ttgatctagg tcaactacca ccatttgatg
    25621 atcttcaagc attaacccaa tatttctagt tgcatcttcc gtccaccatt cattaatttg
    25681 attagaatcg ttagttgcat cattccaacc actagagcct tttattggaa ttttagttcc
    25741 aggagataag ggataaattg accatcccga cttttgtaat tccgttgctg cttgataatt
    25801 atttgccaat aaacctctcc tcctttttca aaatactggt ataaatcatt cagacacctc
    25861 catgagcttc aactgctcaa gttcgtattt caacccatcc cgaacaaatt cagcaccttt
    25921 taattgattc tgatacttta cccatgtatt aaatgttttc acccttgctt cttgcatatc
    25981 agtaatgttt aaccatgcgg ttaaaatctg attttttagc tcgttgattt ctcgacaaat
    26041 tattttgtgt tctgcatgtt ttttgagtac atacaaaact gttagttttt cattgaatga
    26101 atttccaaag taggttttcc cgatattatt ttctagttca gcgatccctt gattaatttc
    26161 ctctaattca aggctatcga gttggtttaa tagttttgtt ttgtacatct acttatgccc
    26221 tccagttctt aattgatagt cgtactcgtc ataagcagtg aagagatata acgctaatcc
    26281 tgcaaagata gctatttttg caattaatgg taataagccg aaaattccgc cgattaaaat
    26341 gcttcctata attaagtttc gtttcataat ggttccctcc gattttttaa tatggtataa
    26401 tcggaacaaa gaatatcggg ttttatccca attaagttgc ctttatcctg tccgccaaga
    26461 ctgttatgga taaggctttt tctttgttct cgatcatgtt aatgctccta agttgtctgc
    26521 aattcagcag aacgattata aattttctca ctcactgaaa actgcaaccc atatttttct
    26581 ttaacagtca ttaattctac tgtctcatct aaaataggtt cacgatcaac caacatttca
    26641 ggagtcatat ctttcttttt taccattttg gaatgaccat aaatagttga tacagcttta
    26701 ttggctatca cattcgcttt aatgtagtct actggttttg gttcagtaat agcgtggctt
    26761 aatttagtca ttgcttcttt ttggtgttct ttgtctagca tgcggaatac ttgaaagcct
    26821 tcaagtcctg tggactggcg tagttctttg atgatgtcga aaatccagtc ttgaaatgcc
    26881 tcggcttctt ttttacggct tttgaatgcc atgcggtaaa tatttttttt cgttgatgat
    26941 gtttacatct tgtgttctgc ccaatgaatc gatgacctta ctagtagtaa ccccatcttt
    27001 aggcaaagag tgaatggctc tagtaacctg tttaagacct aacgcttccg caacatcttt
    27061 cgccactgcc caccattcat cattaatatc gacaaatctt ataatgtgtc cgttccaaat
    27121 ttcagttttc attcaacgtc ctccagttca agccctagca agctcgctaa caactcaact
    27181 tgctgataca aaatttcttg atactcttca atagaaagtc gttctttaaa ctctgcttgt
    27241 actaaatcgt tcaagccacg caatactccg cttaccgttg gttcttgtgg tagcaatttt
    27301 tgatagtcca tttgttcgta caaacattca aacgtttctt ctagcgcttt gccttccaag
    27361 tttgctaatt ctgccgctgt gtcttctgta agaattgttg ataatacttt ttcgattgct
    27421 ttattttgtg tcgattccat tattatttcc tcctattaat ccctttgccc ccacggcttg
    27481 cgtttatttt tggtattctt ccataaagcg atcacattca tcttgtgaaa ttcttacgac
    27541 tccatcaact ataatcaact ttaaaccgtt aggaacatat ttctgcatca tagtattgta
    27601 tgaaacgttc atatacttag cagcttgtgc cttgttcata tattttggaa actggtactt
    27661 tttttctaat aatgaaagta acgttccttg agtttgctct acttgttgaa caactcgatc
    27721 gtcaataatt ttaaaagctg gatcatcaag cgtgagtttg tcaattttta attccatatt
    27781 tatcccccct cgttagcata catttctata caaagatttt aaaaaaatgc aagaggctct
    27841 tgaatacatt tctatacctc ttataaactc aatataccat catacctttt catttgcaag
    27901 aattttaaca tacttttttg aacttttatg tttcacacat atttaaatcg tgatatagta
    27961 gagaaaatat atgaatggtg gtgaaaatgt ggctagaaaa aaataccccg aagcagtgga
    28021 gacaggacaa agaattagac tggctagaat tgaaaaggga ctctcaatgg aacaacttgg
    28081 aggtaaatta tccccaccgg ccagtaaagg tgccgttagt aattgggaaa atggctataa
    28141 tcttcccaat aacaccaggc ttaaacaact ttcaaaggta ttagatgtat ccactaccta
    28201 tctattaaac ggatcttata cattaagaga tatgcatatg atgcccgaaa ttaagcgaaa
    28261 tgtgcttgaa aagcaattga aaaaaactat tgaaaaaaat aactctataa ttgattctaa
    28321 tctttctaat gctatcaata acatcgaaga agataacctg actgtctcac agaagattta
    28381 tttaacaaac gcaataaaat atcttaaaac aggatctcga attgaatttc ctgacgggag
    28441 taccccaatt gcttatctcg cagctcttct aaatacgctt ttacgatatg actcattaat
    28501 taatgattca gaaaaaaata cagatgagaa aaaagagtat caagaatttt atgaaaatga
    28561 tcttctaaaa gagttcacag ctcttgttac tgagctaaat aaacactatt caaaataata
    28621 ataaactgcc tcctccaacc gcccccacgg cttgctacac tatggaggga aaactattat
    28681 ggcaacattc aagcaatatg aaactaaaaa aggtaaattt tggctctatg aagcctatct
    28741 tggaatgaac aaaatgactg gcaaacctga taaggttcgt agacgaggtt acaaaacaaa
    28801 aaaagaagcc cagcttgcct tgtcacgctt gcaagtggac tacgataaaa acggtattaa
    28861 aaaagcgaac aacgaaacgt ttaaagaagt ttatgacttg tggctagaaa catataaaac
    28921 cactgtccga gaagccacct tcatgaaaac tgaaatcaag tttagaaagt ggattcttcc
    28981 aaaatatgga aatcttcgtg tgaatgaagt cacggtaaaa aaagcccaac aaattgtaaa
    29041 tagctgggct gaaactactg accaatacaa ggtacttcat tcaacttcta agcgtatttt
    29101 tagctacgct atcaatcttg ggattattga gactaatcca ctagacaacg tattaatgcc
    29161 taaaagaacc accactaagc ataaagaaaa ggtcaaagtt tacagtaaag aacaactgat
    29221 caccttgttt gattacctaa acagcaaacc tgaaacttat cgaaacgact ttgataaaac
    29281 gttactacgc tttctctttt atagcggggt taggatcagt gaagcccttg cattgaattg
    29341 gtcagacatt gattttcaag aaaatacggt caccatcaat aaaacactaa gccaatccaa
    29401 aaatggctat aaaatcagtg atcctaaaac tgattgtagt gctggggttc ttccactaga
    29461 tgaaagaaca atttctatat taaaaaaatg gcagattaat cagcggaaat atatgctgac
    29521 acttggtgta actgatccga ctatgatttt ttgtggtatc tataaagaac taatcacaca
    29581 ccacgctatt tatgcaagat tgattactat ttgtgaaaaa gctatggttc catttctcgg
    29641 tgtgcatgta gcaagacaca ctcatgcttc aatgctcttg gacgctggag ctactatggt
    29701 agaagttcaa actagattac ggcatacaaa aattagtcag acgatcgatt gttacggtca
    29761 cttggcaaaa gaaacgaaag aaaagactgt tgaaaaatta gtacaacact taaatattga
    29821 ctagatggca tacaatatgg catacagtaa aaaataaaaa aaggagaaac cctatcatat
    29881 caaggtttct cctagcttta tagtctattc ttcttcttcc gccatgaatg tattgaatac
    29941 ttcttcaatc atgtcccatt ctgcgtctgt ttcgatttgt tctaactcgc cttctgttcc
    30001 atcttcgttt tcgatgtatg cataagcttg taattctaca tcttcatctt ctgaaacacc
    30061 agcaggatat aaaagaacat agttacgtcc aaattcttct tgtccgtcaa ctgttagtag
    30121 gatttcatat aatgtttcat ttccttggtc atccaccaat gtgatgtgtt cgtgtccttc
    30181 atggtcgtgg gaatggttat gttctgtcat aagattacct cttttctttt attattaaaa
    30241 taattgctta cctgagccgt ctaaataatt ttgtaagatc atcacagctg ctaatttatc
    30301 gatgactttt tttcgtttgg ctcttgaagt atttgcctgc tctactagca tacgttctgc
    30361 ctgaacagtg gttaatcgtt catcttgata atcaactggc aaggagaata gttctttgat
    30421 cttttctccg tatgacatgg aagcttctgc ccttggtccg atggaattgt tcatattttt
    30481 aggtaatcca actacaaacc ggtctacttc atattgtgct actaattcct tcaaccgatc
    30541 aaagccaaac tcttcttctg cttcattgat ccgaatgatc tctactcctt gagctgtcca
    30601 gccaaagggg tcactgatag caacacctac tgtcttagaa ccgacatcca agcccattac
    30661 tctcataaat tcactccatg atttgagagg tagtccttga caattacttc catgatctca
    30721 tcgcgttcgt gacggcgaat caaatttcga gcatcacgat aacgaggaat gtaggacggg
    30781 tctccagaaa gcaagtagcc gacaatctga ttgattggat tgtagccttt ttcttccaaa
    30841 gcccgataaa caatcgctaa tgtttcgctc acttcttttt tgcgactatc gtcaaaacca
    30901 aaacgtacgg tttcatctgt aaaacccatg attctacacc tctttcccgc aaggtaattc
    30961 cagcttacct tctacaacct tattttactc gaagaaaggg aaaactacaa acaaatattc
    31021 caaacttccg aataaattaa gaaacactat ttgtatgttt ttttcctttt ttctgtagct
    31081 ttatcaaaag tactcttttc ataaagtata cgctatattt ctttgaatgt atgtttttac
    31141 acaatttttt cataatattc ataaaaaatt cgaatagctg ctactaattt tttgtttttg
    31201 ttgcttgtcc tgtctcataa ttgatcgtgt agccatcttg aacattgtag ataaagacat
    31261 tgaattcaat ttttttgtct tcaatgcttt ctgcttccaa ttgcactccc ctggctacca
    31321 gttcattccc ctcaaaatga ggagtgacac ggtagcgtac atgatgattt gtttctttga
    31381 tatatgccgc aatatcattt tcgtgagcaa gcatgtatgg agcattcaac gaacgtgtcc
    31441 ctgtcatcaa gtttttaatg ttgttatttt gaccagtcag ctggaaacca attaaatggc
    31501 tgcgattata taaccattgc ccgtcactta atttcttttg tttccaacct gtcgggtcaa
    31561 tatacaaagg ctctctcttt tcagtaggga acaaatcttt tccaagcata gcattggctt
    31621 gtcctactct gttcaattta tcaatatttg aatagctttc ccatgttcct ttggaaaggc
    31681 taagatcctc ttttgtaaat tccggctgat tcccattgat ttctatttct tgatgttttc
    31741 catcatattc tggcacatcc gctacttgag aagcttgtgg tgtggaatca aaaatagttg
    31801 taatctgctg aaagatatct gaatcttttt gtgtggtgga aacaccgaat gcccctgcaa
    31861 tcacagcagc gacgatcccc acgatcgcct tggttgtttt tttgctcatt tctttactcc
    31921 tttactaaca gctccctaaa tcttaacata tgttccccta taaataaata ctagaaagaa
    31981 atagaaacag caaaaaataa aacctgtttt aagatggctt aaaaatacaa aaaagagacg
    32041 gattcctcgt ctctaatcag tcaccactct atctgtaagg tatttattat tattcaaaaa
    32101 ttttcttcat actcaagaca atacactgat ttcacaaccg cttggtaaac ggtgcgcaaa
    32161 agcttgcatc tgcggtaata agtccatcca agttaaaaac ataagaagtt gtttttgctt
    32221 ggctgttctt attacttgtt gcagagcagc tttttcacaa cctattttaa gcgggtgacg
    32281 ggaatcgaac ccgcgacatc agcttgggaa gctgggattt taccactaaa ctacacccgc
    32341 aatacaaaaa cagtatacgc ctcttttatt ccttcctcaa gtttttttat tctttacgct
    32401 tctggtatgt tttgtagtaa aataaaacta atcaatcaat tattggagaa gatgctatga
    32461 cgggaaaaga attagatctg ttgctggaca cttgtttact cgctgggaaa atcatgatgg
    32521 aaagcaatgc ggagatgtat cgggtagaag atacgatgag tcgaattgct ttagcttctg
    32581 gaaatttccg tttggtcagc tatgtcactc aaaccggtct ctttattggt cttgatcgta
    32641 cctcgaccat ccgaatggaa caaattacca accgttcgat taatttagaa aaagtagtaa
    32701 aagtaaatga tctttcacgt aaatatgttg cttcagagct aacattagaa gaactttact
    32761 gcgaactgca agcaatcgac cgagaccgtt ctttctttcc tgtctggttg caaatcgtta
    32821 gcgcggcagt gatcagtgga acaattatgg tgttatttgg cggaaagatg accgatttac
    32881 ctgtgacttt agtcattgga ggattgggat acgcagctta tctatatagt ttgaaatttt
    32941 ttagaatcaa atttttatcg gaatttttat cttctttatt gattggcata gccgctatct
    33001 acagcgtacg ttttggctta ggaagcaatc aggatctaat cattatcgga tgtgtgatgc
    33061 ctttagttcc tggagttcaa attacgaacg ctatccgtga tttgctagct ggacattatg
    33121 tctcaggagt ttctcgcgga acagaagcga tgatgactgc tacgatgatc ggttttgcga
    33181 ttgcttttat tttccagctt ttttattagg aaaggaacac aagtatgggg acgatattta
    33241 ttcagttttc ttttagtttt ttagcgactg cggcatttgc cgttattaca aatgtaccta
    33301 ggcgttcatt gatttgttgt ggtctttctg gtgcttttgg ctggatgatc tactggatct
    33361 gtgttcaaat aggcggatct gctgcttttg gttcattgct tggttcttta ggagtagcat
    33421 tcatcagcga tcttttctct aaacgtttga aaatgcccgt aacgattttc aatattcccg
    33481 ggatggttcc tctagtccct ggtggtttag cttatcaagc ggttcgaaat ctcgttactg
    33541 aatcctatcg agaagcggct cattatgcgg tccaagcaat tatgattgct ggagcaatcg
    33601 ctttaggtct tgtattatca gaagtcttta accataacat ccgtaatttc aaagtaaagc
    33661 gtgacgcaat catgatgaaa cgaaaaaaac ggctttttaa ataaacgaag tgtaacaaag
    33721 aaaaggttgt gaaagagacg ttcagccttg agcgatgaca aaatatctca caaacattgc
    33781 gacaagtaac cgcaggagca ataagaagta attatgagta ttaaaaaaag ttttgtaaaa
    33841 atagcttctc acatttttac ataaaattga cttaatactg agaaactgac ttttgaacac
    33901 cctctatctg taaaaaaggg gctgcgacat gatttttgtc acggcccctc ttctttattc
    33961 aaattttttc caaaacgttc gttgataata aacgatccat agagcggaaa gtattagaac
    34021 taaaccgatc acaggaatat tcactaacca aaatggataa aacaaagcca acattccgta
    34081 taagacgata agaataatac tgatgatcaa tgaacccatc atagttgcga ctaaacccaa
    34141 acttcctgaa cctctgttaa ataattggtt aatattggtc cagttcagca gcagcaaccg
    34201 ataatctcta gcgaaataac gtagtgacaa taagtagcag ccgaataaag aaccaaatag
    34261 caagctaggg ataaaaaaca aaggcagctg aaaagacagt ccgcttaata atgcgatccc
    34321 accagttaaa aaactctgca atatccatcc gaatctgaac ttttctttta aatattggtt
    34381 catagagatt ggcaaagaac ggataaacag aaaattttct tgatccaaag aaataagatt
    34441 gctgataaaa gaagtctgat taactgttaa gaaagccaat gcgatcccta ccaggaaaac
    34501 aacaccgatt aacttattat ctaaatcagt taattttatt tcgcctccta gggcaaaagc
    34561 aagaataaaa atgattggag ttaacaagga agaagaaaga acttgcataa tcaaattagg
    34621 tttttttact aattgagcat tgtaactaaa taaaagctga tgcacatttt ggttcttttt
    34681 atgggttctt tttattttga actcactgtt tgaatcagtc agttgctctg taagctttgg
    34741 tactatcagc tgttttacag caaggaaaaa tcccagagcc gtgatagcta gtattcccca
    34801 acccaataaa ccttgttgag aaagtggctg actggcagca tcatagaatg gcaataatat
    34861 cgaaatcgga tgccgatctg ttaactcatt ttcaatagtc cccgtttgat gattcatcca
    34921 catgattcca gcaaatgcga tcagcatcga gctgccaagc aagatactag aaactgtttt
    34981 tctatgtttt ttaaacactg ctgttcgagc caaaccaaaa acaatcaaac tagccagaca
    35041 aaaaagaata atcaaaaata gagaaaacaa aaggattgag ataatgatag taagcaaaat
    35101 ccctagatca gctcgccatc cggttaaaat aaacatgact agaagcggaa aaacaaaagg
    35161 aatgatggtc aaagcaacta ccaaaatctt tgctgaaaaa acctgttctt gtccaaaagg
    35221 cagaggcaaa aatacttgca ggtcatttcc ttcaaaaaag atattataaa tgactgatat
    35281 actttgagtt aaacctaaca taccaaaaag tgccacgtaa taagtaaaga aacctggcaa
    35341 tttaacaaag ttcatagcaa acatagcaaa tccgtaaatc agtaaaaaaa tcagccctga
    35401 aaaaagattt tgagacaaaa gatagatagt taatcgcttt cccttttttc ctttctctct
    35461 tgctttattc gtgagctgtg gattaacata taaaagatta actcgaatta attctaataa
    35521 ttgtcgctta tacatacggt ccatctcctg tcgcttgcct tccagccatt ttcaaataaa
    35581 tcttttctag cgtttcatca ggtgagccat tcaataattc actaactgcc ccctgataaa
    35641 tcaattcacc tttcttcaaa atagccagtt tatcgcataa ctgttgtgcg gtatccaata
    35701 catgtgtaga aaaaatgact gtttttcctt ttgcggcatg agccttcatc atttctttca
    35761 aatcaaatgc tgcttgagga tcaagtcctt gcaaaggttc atccaatacc caaatatctg
    35821 gatctggcag caacgtgccg atcaaaatcg ttttttgacg cattccatgt gaaaaacttg
    35881 cgattgtttc attttgatga ctatacatat caaaaagcgc tgtcaattct gctaaacgct
    35941 tttctttttt ttgtgtgtct aattcatagg cagctgaaat caagtcccaa tattctccag
    36001 ccgtcaattg taaaaaaata tctggggaat caggcacata accgattttt tgtttgatac
    36061 tcaatcgatt ttccgtcaat ttcattccat ctactaaaat cgttcccgat gatggttcaa
    36121 taatactaac taaactttta atcgtagttg attttccggc accattatgt cctaaaaatc
    36181 caaaaatttc cccttgtctt atcgagagag acaagtcttt taatgcttgt tttttcccgt
    36241 aacttttact tacattgatg aattcaatca tttctgtact ccttttgttt gttattactt
    36301 tttattgtac ccttcgttat tggtggagta aatgtcaaaa agagtgcata acaaaaaagg
    36361 atagaaacga ctaggctgct acttgggttg ttctcttcta caggaaaaca ctcttctaac
    36421 aggtaagata tgagtctttg ttttctttaa aatgattcat tttctatctt ttcttgcata
    36481 aaattcttta gtttatcaat acttctcttt cccatctcta gttgttctcc atttgcaaat
    36541 gttactattt cgtttactct gctaatagat gtaatgtttt cagtattaat gatataagct
    36601 tttaaatctt tacataaata ctcatattcg agtgattttt tatatttttt aattgtgccg
    36661 tttacgagaa tctcttcatc tttcaatact aggcctattt tatttttata acctggcatg
    36721 ctcattagat aaaggatttc atcgtaatgg atatagtgat cgctattacc ataacggaca
    36781 gttgtccacc tttttttcgt agcatgttga acaggatagt tttctttttc tttcaataag
    36841 taactttgga tgcttttttt atttttttca taagtgaatt cttttatcaa ataaccacta
    36901 ggcgtaatct ttcgattaat aacagttatt gctttattcg gatgagacgt tagataaaca
    36961 attttacact tttcattctt ttctctaagc atttcgccta gttcgatacc gctataatag
    37021 gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat agatttcatt atttttaatg
    37081 aattcaacaa attgtataac gttcaatccc tgaacgatct caaatttcca aggacttaat
    37141 aaaattctgg attcttccaa aatttctact agcatttttt caaaaaccaa actatcatcc
    37201 aaaataaata tacgcgttac cataaaaagt acctccactc gatattttac tatcaaaatc
    37261 atttcataca acttttcaac atacatgtta ggaataaatt gtaattctct tcacaatctt
    37321 ttccaacacc tttgaaaccg atatcaaata ctttcaggat gctgcttcta aaataaatat
    37381 caaacaaatg aatacatcat gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct
    37441 gtctgagcta tgtttattta ttttgttctt ttaaaaagaa cgatacacat actgattttt
    37501 cggagcagag actttctcag cgtttgtaac aactacagat gggatttcac gcttgaatgg
    37561 ttgataatag tctgattgga tggttccttc tattttatac cattcatcat ttttaaccgt
    37621 tactccttcc ggcatatgcg tcaacaaacc aaagactcct gaatctgcca cacaatggat
    37681 aatgccgaat cggaacaaga agatatctgt attgctatcg ttcggtgttt ggtaaacaaa
    37741 tccttcatag ctgatttttt tgccaatgaa ttcgctaggg taattataaa ttagctccat
    37801 aacttctaaa tagttttcat ctgtgatctt gatagtttgc ttatccccat acttttcttt
    37861 caactcattc atttggtcta agtagtctga tttgttgaaa taaatactgg tatccggctt
    37921 taagtattgt gtctgcatct ccggatcccc tactgattct ttacttactg ggaaattaaa
    37981 tcctttggct tctacgatcg tagtgtcgag actaactgtt ggaaacaacg tccccacgat
    38041 tactggcaaa gctagcagta cataagcgat tccacgttga taaggttttg acaacccatg
    38101 gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc acaaatacag
    38161 ctgaactaaa gccaaaagag cagataaaat cattgaaaga atagctaagt agcgataatg
    38221 gacattgata tattgatcga gtttgccaga aacttgcaag tacatcatca attcgaaata
    38281 cccagataaa atcaaaaacc gcagcatatt ttccctccta aataaataat gtgaatccaa
    38341 taacgctaag ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt
    38401 tcatcatcag tagatttttg atatcgacca ttgggccaaa gaccaaaaag gcagctacag
    38461 gtgctgtgcc gaacagactt aacaaagaag atcctataaa cgcatctgct tctgagcaaa
    38521 gagacatcgt aaaggccaat aacagcatga tgataatggc taaaatcttt gttgagccga
    38581 gcgttagcat aattctagtt ggtaaataag tctgcatcgc agctgcgatt aatccgccaa
    38641 tgatcaaata acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag
    38701 tttgatggat cagctgattt tttggttcat tcttctcact ttgttcttct tcatggacat
    38761 gatgatggtg attttctgct actttagacg tgattttttc tttcaataca gcgtcttttt
    38821 gaaaataggc aagccagaca ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc
    38881 agcttgccat ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc acgattggat
    38941 tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg aactggtacg ccttttttaa
    39001 caaattgatg aacgatcggt acgatgccgc attcacatga aggaaagaaa aatcctaaga
    39061 tcgaaccagt aaaaatagag aagatacgat tttcaggaag ccatctcttc acacgttctg
    39121 gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc taataatacg aaaggtaatg
    39181 cttcaataac gatcgataag aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag
    39241 aaaacatgag tttcctcctt tttatttcta gttcattcgc tttgcaaata tacgctttta
    39301 ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact cttatttttt accaaagcac
    39361 ttcaattgac tttttctgtt attcccatta aattaaggtc tcatacatat cacttctatc
    39421 ataaaaggga gaataaaaat gaaacaaacc actaacttga ctgaagttca agacatactg
    39481 caacagtcat ccgttgccgt catttatctt tcgatgccta attgcagtgt ttgtcacgct
    39541 gttcgtccac gactagagga acttctcaca cattatgata ttccggcact tcatttggat
    39601 gcatttgaaa caccagaagt agctagtaaa tttgaagtgc tgactgcgcc agttgtcctg
    39661 atttttcatc aaggaaaaga agtctttcgg caagcacggt tcatcgactt taaaaaaatc
    39721 cgttatctac tagatgaact gacagcagaa gatgattccc tttcttatga agaaatcttc
    39781 cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa agttttgcct
    39841 tcatttgttc aatcgctttt tatttttatt ctgctgtatt ttcggaatgc gcatttgcgt
    39901 cagaatgcaa gaaatagatc aaggtttgca attcattcgt caaatcaaca ttttggatca
    39961 atacatctga cggagcattc aatcgagcag cggtaaagtt caagattcct ttgattcctg
    40021 cttccgttaa ctgattgact acttcttgtg ctctttcagc gggtatcgtt aaaattgcta
    40081 cttcgatttg ttggatcttg atttgttctg acatatcttc cattggataa acggggatac
    40141 cgtctacgat gcgcccaacg atttcctcat tcacatcaaa agcacaactg atacgtatgc
    40201 tattactttg atggaatttg tattttagta aagcacttcc caagttccct acaccgatca
    40261 gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc ataagatctt
    40321 ctacatcata accataccca cgttttccta attcgccaaa ataagaaaaa tctcgacgga
    40381 tcgttgcact atctacttga acggcttcac ttaattcagt tgaagacact tttttctttc
    40441 cagcattatt taaaatcctt aaatagcgat aatacaaagg cagtctttta gctgtcgcct
    40501 ttggaataat atgatctttc acgaaatgac ctccacgtat tgtctaatta catctttgat
    40561 ttctccatca tagcagtatt tataggaaaa aatcaattcc attgctcaga tattgtgaaa
    40621 ttttattctt tattcattta tttatcattt ctgcaaatct cgcagtataa tggaatttat
    40681 ggtagactac taattgaaaa atagtttttt atttgaggag aattcacaaa gtagaaaaga
    40741 ggattccatg atattattac aagccaatca gattgctcgc cattttggtg cagaagtctt
    40801 atttgaaaac atacatttag aaatcagtac aggcgctcgt gtcgctttag ttggcagaaa
    40861 tggcgctgga aaatcgactt tattaaagat cattgctgga attgaagcac ctgatgaagg
    40921 aaagatcgct aaaaataaaa cagcgactct cggatacctc gctcaggaca caggacttga
    40981 ttccaatgaa acagtttgga acgagatgct gaaagctttt gaacaagtta gacaaatgga
    41041 agagcggatg cgcgagctag agattttcat tagcgaaggt acgccagaca cacctgctta
    41101 tagttcttat ttaaaagaat acgaccggct gcaacacgat ttttcagatc gaaacggcta
    41161 tggttatgaa aatgaaattc gttccgtatt gcatgggttt aaattcgatt cttcatttta
    41221 tgatcagaaa atcgatactt tatcaggcgg acagcgcaca cgtttagctt tagctcgcat
    41281 gcttttgcaa caccctgaca ttttgattct agatgaacca acgaaccatt tagatatcga
    41341 gacactttcc tggcttgaga attatctgca aagctatact ggtgcattat tgattgtttc
    41401 ccatgaccgt tattttttag ataaagtagt caatgaagtc tatgaattaa gcagaagaaa
    41461 aatgcgccat tacaaaggta actattcgaa atatctagat ttgaaagcag aacagttagc
    41521 aagtgaatgg aaggcttatg aaaagcagca aacagaaatc cataaattag aagacttcgt
    41581 tgctcggaac cttgttcgcg cctctaccac aaaacgagcg caaagccgca gaaagacatt
    41641 agaaaaaatg gaccggctgg atcgtcctca aggggatgaa aaacaagcaa actttctatt
    41701 cagtatagaa aagccttctg gaaatgtagt cttgcaaatc gaagatgccg ctatcggata
    41761 tgataatgtc accttatctg aaccaatcaa tctagatatc cgtcgtcaag aagcgattgc
    41821 tctagtaggt ccgaatggta tcgggaaatc gacgttgctg aaatcaatca ttcaccagat
    41881 cccttttatc cgtggaaaag aaacgctagg gacaaatgtc accatcggct attacgatca
    41941 aggtcaagct gatctgcata caaacaagac gatcctagca gaattgtggg atgagcatcc
    42001 taccactcca gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt ctggcgaaga
    42061 tgtagaaaaa ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg ctttagccaa
    42121 gttagcgatg aacaaagaaa attttttgat tttggatgaa ccaacaaacc atttagatat
    42181 tgacaacaaa gaagtattag aaaatgcatt gatcgattat gacggcacac tgctttttgt
    42241 ttcccatgac cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac tctctgaaaa
    42301 tggcagcaaa gtctatctag gagactatga ttattattta gagaaaaagc aggaagaaga
    42361 agaaatcgcc gaattgttgg cacaagatga gccagaatct gctcctgctc ccacgaacaa
    42421 atctgattat taccaaagca aagaacagca aaagcttatc cgtagccttc aacgaaaaat
    42481 tactcaggtt gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt tggaacaaga
    42541 aatggtcgcg ccagaaaacc tggaagatca cgtaaaactc aatgagctga accaagattt
    42601 agaagctgct agacaagatc aagaaacaaa attgacggaa tgggaagaac taagcttgga
    42661 attagaagaa tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat gctgttttgg
    42721 tttttctttt tttattttat caaacaaaaa ttccatattt acctttttca ccaataagaa
    42781 gaccaattac tagtcattct cactaaagct tctaaataat agtaatcacc ccaaatacaa
    42841 ggtgtattta ccccattatt tttgggctta ttataaacag actgtgttaa aaagccatct
    42901 tcttcttccg ttgtagcata tctttctttc aattgtcgca taattttcca aacagcagac
    42961 tggtataagt tcttttctgt tttgtctaat agacctgatt ctattaattc taataatcca
    43021 caaaccatga tcaccatact agatgtatct ttttcttctc tgctagaatc atcaaagatc
    43081 aaatcccaat aacaaatggt atcttctggc aaccgattca taaaataatt ggctaatatt
    43141 ttgcttaaat caaaatattt ttgttctttc aaataacgat aacttaatgc aaaaccgtaa
    43201 atcccccatg cttggcctct agcccaacag gaattatcac tataaccttg atgcgtactt
    43261 ccatatcttg gttttccatc tttgacatcc atataaaatg tatgataagt tgaaccatct
    43321 tctcttacta aataatctgc tgcattttga atatgagcct gagcagcttt tttatatttt
    43381 tgatttccag tttcttcact cgcccaatac aataatggaa gattcatgca acaatccatg
    43441 atcattctac cttgttgtga aggatcacta ggatctcccc aagcttgtat aactcctgcc
    43501 ttctcccaaa aacgctgaag taatgtttct gcagcatcaa ttgaagcttc cttcattttt
    43561 cgatttcccg ttattttata atctgctacg gttgataaag aatacaaaaa accaagatcg
    43621 tgatgattag tgataatttg acagtcaatc ctgtggataa aatctatatc tgctttttca
    43681 gctgtttgtt gaaataaagg atcacccgtc ttctcaaata acaaccaatt aattcctgtc
    43741 caaaatcctt ctgtccaacc aatattgtct cttaatggat aacgtccttt tataggacac
    43801 tcgcttggga attgatactg actgttactg atacgtttga ttaacctctc cgtgtgtctg
    43861 cgacaacgct cgatctcatt attccattct ttttccatta attctttata acattcattt
    43921 atccattttt ctggatgtat tatctcttca aacatcactt tatcaagcat ttccccacca
    43981 gcctttagca taattttagt ctttttttat tgtttttatt ttggatagtt agcccggttg
    44041 tcccatcaaa atcaattttg ggacgattgt tccatacttc taaactataa tcccgatctt
    44101 gatgagcaac cactcctgtt ccgagccaat gggttccttt tccaaatgta ccctctaatc
    44161 caggaataaa agtgttttcc catgtcatca agtttgtatt gggcaaaccc ataatagctg
    44221 tggattttcg tttaatagta tcgtcactta aacagataat acctgaaaag ccagcttcac
    44281 tcaccctata gttttccttg tcttcttcta tgattattcc ttctttctca ggagaacatt
    44341 caatagcaaa ccctgtctca tatagaacat attctgtatc taattctact ttgtgtacgc
    44401 gaatctgcca gccttcaaaa ggaataagcc aagttctgat ttttgcctgt aaagtaggtt
    44461 cccatgtaga ggcaagtacc tctttcttac atacaatgtc tcgtacatta tttcgaacga
    44521 tgaactgtcc ataggttttg atgctctcat tcttaaaact ggtcgtgata tatgtgtccg
    44581 ctcgctgaat ccctagcatt gaatccattg ccatttctgc tttcaattga ttcgctcgcg
    44641 gtatcgaaaa accaaattga ctagaataag caaattttaa atatttttca ttggtcaatg
    44701 tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca ttatcatgtt
    44761 gtatgagcaa attggcttct gaaagaagtt tagttccttc acgaataggc ataggcattt
    44821 ctttagccgt ccaatactca tggtcttcag gtagcgccaa caataaaaaa atcttattca
    44881 gccataatgg agataacgta ctattatagg gttcagtcaa tgtcaattga gggtaggtat
    44941 aaccaatatt caggatacct ttttcatcaa atatttcttt ttgcatccac catcttatat
    45001 tccgattgac taaccccttg atttcaccta atttaaaagg caaaagatcg gcccacaaca
    45061 tagctgacca aaagcttgaa actgcaaaac gataaatcat gcttcgacca aaaggcacat
    45121 ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga aaaatcaata gctcggttct
    45181 tatatatctg gctaatgggc ttttctggtt ctagtacgct ataaatcaaa ccataataat
    45241 gaaatgcaaa aggattataa taatcttggc gacctcttga actgtctcga taccagccat
    45301 tttgaagata acaattttct atattatcaa atgcttcttt cagtgctgat tctttaactg
    45361 gataaccaaa tttttggata attgttccga caattatctt aaaaaattgc caattaccat
    45421 ctgcacattc atattcaaaa atttgcgaga accaatgaat aatctggctt ttttctcctg
    45481 tatctaattt tttccataat aattgttcat gatgatataa cgctaataac attgcgggca
    45541 tttctaccat acgctggtct ctttcatgta tttctcccca ataatcggga tgggaaggat
    45601 ctgttccagc ttttattcct gatagaatta atttcaacca tcgttcatct aattggtcaa
    45661 ttgcgggccc cgctccccat aaaacccgag agaatccttc cattcctgca attttatttc
    45721 catatcctac cccttgatgt atataatcaa tttgtgcgcc acttgctcga taaaacggct
    45781 ggagtggttg atttatcttt ttaaataagt caaagtaatc ctgtctggtc cttaatagtg
    45841 gtactggatg tttcattgta ttttctcctc tttttactac caaatagcta attccgactg
    45901 ttctaatcga agtaagcctt caataaaaaa ataatcagaa tagatcagtg gcacattata
    45961 agtcagttct ctatggtatg cttctgctcc gcctccaatg atcccatctt catccatatt
    46021 ccaatttgca tagtgctttt ctgtcttttg gagtatattg atagcagctt catgatacat
    46081 ttttgatcgt acagaggaaa catatcctgc taattccaga agaccgcatg cagcgcatac
    46141 tccagcactg gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag gaatataacc
    46201 agttttttcg attttctgaa taaaatttac tgctatcttt tctgctatag ctagatagta
    46261 cggatttttt gtgtaacgat aacttaacgc gaatccatat aaagcccatg catttcctct
    46321 gctccaacaa gaattctcat tgtatccttg cccgccaagt aaccgaatat atttccctga
    46381 ttctgcatca aattctgcga tatgcccggt tgatccatct ttcctcacta gatacttagc
    46441 tgttgtatct gcatgctgcc gagcaatttt agagaacctc ggatcaccag tttccctcga
    46501 agcccaatac agtaatggta aattcatcat actatcaata atcatccaac caggtttatg
    46561 gtcattccat gctactaaat agttccctga aatattatag cgtccagcaa gtaccgtagc
    46621 tgcatgtaac cctagttgtc gactatgttt gttttgagac tgttgataat gtgcaatagc
    46681 cgatggtaag aataaaaaac ctacatcatg atgaacgtct gaaaaatggt acagggcaga
    46741 ttctaataat tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt ttgtcttgtg
    46801 aaagagctgc cataaaatac cactccaaaa accattggtc caccagtcga ttcctctgtc
    46861 ctttattacg tctgtatatt ttccgttgta agaaatatat ggaattttat tccccattct
    46921 gtctaattcc gcatctatct tttcagaagc tacattcaaa atttttcttg accattcctt
    46981 cgtctgttca ttcatggata tcccgccatt cttttaattt ttatgaatgt ggagttgctc
    47041 ctagaacgaa ctagatggtt aacattcgtt atttttttta cttatttgca gctccaatgt
    47101 cattctcctt aaatttagac tgcgcccaca cataaatcaa gaccattggg atcactgaag
    47161 ttgctaaaat tagcataatt gcgccataat taattggctc acctgtacta gaaaccgtca
    47221 tatttttgat aattaaggga accgtatatt tatctttatc tgaaagcatg actaatggaa
    47281 atagatattg attccaactt cccataaaga taagtaaacc actagtaata atcgtaggtg
    47341 ataaaatcgg tacagcgatt ttcgtaaaaa tcacccactc attggcacca tcgattctgg
    47401 cagcctccat cagtgctgta ggaaattgac tgctatattg tcgcatcata aacactgaag
    47461 ttgtactggc aatcgaaggg atgatcacac ctgcataagt attcgataat cctaaacgac
    47521 tcataatgta aaataaagga atcaatgtgg taaaaaaagg caccatccta gatagcataa
    47581 ctgtattgaa caacagttta ttccccataa aacgatactt tgctaatgca taacccgcca
    47641 ttgtagaact ggctgtagct aaaactgtac tgattacagc aataacaatc gaattccaaa
    47701 taatcaaggg ataattatat ctcgccatga tggcttgcca gttttttaat agcatatccc
    47761 caaacttgaa attgattccg ccacttaaaa tttcttgatt actttgcgtt gaagctacaa
    47821 tcatatataa atatggcatc aaaaatatta aggcaccaaa cacaataatg acgtacgtaa
    47881 aaattctgct ttgtatcttt tgtttagtca tttttatcac ctccgaattt aaattgaata
    47941 gccgataata taacagatac aaacaccatc gtccaggcaa tagcagaggc ttttccaaaa
    48001 tcaaccattt caaaacttgt tttatataaa ttgactccta cgaccaaagc tgaattttct
    48061 ggtccattgg aagtcccaaa gaatatattt gggatattga aattacccaa gccattgata
    48121 gtggctaaaa ctgtggccat taaaatgatt ggctttagca tggggatcgt taataaaaag
    48181 aattgccgaa atttcgatgc tccatctatt tcagcacttt cgtatatctc tttagaaatc
    48241 ccttgaagac cagctaaaaa taaaatagtg ttatatccta cactaagcca aatcatcgtg
    48301 atgatcattg atattcgtat ccaaaaagga tttgttaacc actgtacacc gttaattcct
    48361 agcgcattca aaaatccatt gaataaccca gtcggattga aaaaagtcat gaatacggaa
    48421 gctacagcca ccgttgatgt aatggttgga atataataga cagttctaaa aaatcctttt
    48481 ccttttaact gtgctgaatt caataaaaca gcaaagtaaa tagctaatag catcgttatt
    48541 ggaattgatc ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa caatggatct
    48601 cggaagatag ctagataatt gtcaaatcca acaaattcaa atgagtagcc aattttcata
    48661 aaactaaaag caaacgagat aatgatggga atcaaaatcg tcacaataaa gaaaataaca
    48721 aaaggtgtga ccaatccata agcacttttt ttatgagacg tatctgttgc ttttttcttg
    48781 ctgttcttta ttgtcttgct tggtaacgaa gattgcatca tcgttaagtc ctcctaatat
    48841 tttttaatca ccagttaatt ttacgccgta cttatgagca aaattttctg cttcttttag
    48901 atatgaattt tcaaaatcac ctgtttgcca atagttatat gcagcggtag tcaaataaga
    48961 cgtagcatct gaataagtgg ctggtaaaac agtatttgat gtattttcat tgattttatt
    49021 caatagttca tagtattttt gtcctcctta gtagtcaaac tttttctcag ctgcttctgt
    49081 ttgataggcc aatgagttag atgtagccac gcccagctcc agtgcgttgg ctaagcattc
    49141 ttcatctgtc agcataaatg taagaaattg ttgtgccact tgtggatttt ttgcatcttt
    49201 agggacataa aaacttgatc ctcctgatac tgcacctttt cctggatttt cttttgaaaa
    49261 aggaatcagt ggagccatgc gccatttgtc agatgcatca ggaaaattca aagacatatt
    49321 cgtagcatac cagccgccag caacaaacat tgcagattct tgaaatgctt tctcagcatc
    49381 ttgtgcaccg tagaaatgta caatatctga gtcgatcatt tctttgatta actttgcagc
    49441 atgtttcgct tctttagtat tcagacggat atttccttct tcatcaagca aagattgatt
    49501 ctgctgggct agcatgttga taaacaattc ttggtcccca gattctggca aggcaattcc
    49561 aaaactacca gttttttctt ttaatgcttt tgccacctgg atatattctt cccaagaact
    49621 gatttcttca aaatctacac ctatttcagc aaattttttt ggttgataat aacaaagtgc
    49681 tggggtaaaa tcactagccc aaggaataat cgtttcatta ctcgtttgtt gacgcaaaat
    49741 attcaatttt tgatcataaa actgatcgcc atatttgtca taaaatccat attcatcagc
    49801 actataaaaa gcatcttgaa atttttccag cacacctgct gctgaagtgt ctgccatgga
    49861 gataatatcc ggcatatctt cgtgtgttac caattttggg gtgatcattt gtactgttgc
    49921 tgttcccgaa gctacattca caatttttaa gtcaatattt gctccagttt tgcggttata
    49981 gatttttgca gcattttcaa catagtttgt cagtttgccg tttgcgacat atacttctaa
    50041 tgtagtggct ttttttatca ttggatctgc gatgtttgct gttacttctt cgttagtttt
    50101 tccacatgca cttaagctga ataatagggt aatgtttagc aaagctatca ccaatttgta
    50161 tctttttttc atgtctatcc cttctttctt ttcaagttct tat
//
LOCUS       DAYYAG010000007        48299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000007 DAYYAG010000000
VERSION     DAYYAG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.007"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHARQ_000351"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHARQ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8408806.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHARQ_000352"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHARQ_000352"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8408807.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHARQ_000353"
     CDS             complement(2298..2960)
                     /locus_tag="ACHARQ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8408808.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHARQ_000354"
     CDS             3144..4790
                     /locus_tag="ACHARQ_000354"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8408809.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHARQ_000355"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHARQ_000355"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8408810.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7524)
                     /locus_tag="ACHARQ_000356"
                     /pseudo
     CDS             complement(5971..7524)
                     /locus_tag="ACHARQ_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7672..8813
                     /locus_tag="ACHARQ_000357"
                     /pseudo
     CDS             7672..8813
                     /locus_tag="ACHARQ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8939..10018)
                     /locus_tag="ACHARQ_000358"
     CDS             complement(8939..10018)
                     /locus_tag="ACHARQ_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8408811.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10121..10561)
                     /locus_tag="ACHARQ_000359"
     CDS             complement(10121..10561)
                     /locus_tag="ACHARQ_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8408812.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10618..11028)
                     /locus_tag="ACHARQ_000360"
     CDS             complement(10618..11028)
                     /locus_tag="ACHARQ_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8408813.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11208..11642)
                     /locus_tag="ACHARQ_000361"
     CDS             complement(11208..11642)
                     /locus_tag="ACHARQ_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8408814.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11742..12590)
                     /locus_tag="ACHARQ_000362"
     CDS             complement(11742..12590)
                     /locus_tag="ACHARQ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8408815.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12979..14745
                     /locus_tag="ACHARQ_000363"
     CDS             12979..14745
                     /locus_tag="ACHARQ_000363"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8408816.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14847..16106
                     /locus_tag="ACHARQ_000364"
     CDS             14847..16106
                     /locus_tag="ACHARQ_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8408817.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16253..17554
                     /locus_tag="ACHARQ_000365"
     CDS             16253..17554
                     /locus_tag="ACHARQ_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8408818.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17557..18408
                     /locus_tag="ACHARQ_000366"
     CDS             17557..18408
                     /locus_tag="ACHARQ_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8408819.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18421..19236
                     /locus_tag="ACHARQ_000367"
     CDS             18421..19236
                     /locus_tag="ACHARQ_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8408820.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19387..20553
                     /locus_tag="ACHARQ_000368"
     CDS             19387..20553
                     /locus_tag="ACHARQ_000368"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8408821.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20783..21430
                     /locus_tag="ACHARQ_000369"
     CDS             20783..21430
                     /locus_tag="ACHARQ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8408822.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21514..22458
                     /locus_tag="ACHARQ_000370"
     CDS             21514..22458
                     /locus_tag="ACHARQ_000370"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8408823.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22593..24044
                     /locus_tag="ACHARQ_000371"
     CDS             22593..24044
                     /locus_tag="ACHARQ_000371"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8408824.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24065..24901
                     /locus_tag="ACHARQ_000372"
     CDS             24065..24901
                     /locus_tag="ACHARQ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8408825.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24901..25560
                     /locus_tag="ACHARQ_000373"
     CDS             24901..25560
                     /locus_tag="ACHARQ_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8408826.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            25686..26375
                     /locus_tag="ACHARQ_000374"
     CDS             25686..26375
                     /locus_tag="ACHARQ_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408827.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26381..27337
                     /locus_tag="ACHARQ_000375"
     CDS             26381..27337
                     /locus_tag="ACHARQ_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8408828.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27381..27893
                     /locus_tag="ACHARQ_000376"
     CDS             27381..27893
                     /locus_tag="ACHARQ_000376"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8408829.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28149..28697)
                     /locus_tag="ACHARQ_000377"
     CDS             complement(28149..28697)
                     /locus_tag="ACHARQ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8408830.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28786..29967)
                     /locus_tag="ACHARQ_000378"
     CDS             complement(28786..29967)
                     /locus_tag="ACHARQ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8408831.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30203..31711)
                     /locus_tag="ACHARQ_000379"
     CDS             complement(30203..31711)
                     /locus_tag="ACHARQ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8408832.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31870..32061
                     /locus_tag="ACHARQ_000380"
     CDS             31870..32061
                     /locus_tag="ACHARQ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408833.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32264..33181
                     /locus_tag="ACHARQ_000381"
     CDS             32264..33181
                     /locus_tag="ACHARQ_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8408834.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33174..34247
                     /locus_tag="ACHARQ_000382"
     CDS             33174..34247
                     /locus_tag="ACHARQ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408835.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34395..34913
                     /locus_tag="ACHARQ_000383"
     CDS             34395..34913
                     /locus_tag="ACHARQ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408836.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34894..35286
                     /locus_tag="ACHARQ_000384"
     CDS             34894..35286
                     /locus_tag="ACHARQ_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8408837.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35362..36297
                     /locus_tag="ACHARQ_000385"
     CDS             35362..36297
                     /locus_tag="ACHARQ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8408838.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36430..37209
                     /locus_tag="ACHARQ_000386"
     CDS             36430..37209
                     /locus_tag="ACHARQ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8408839.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37202..37519
                     /locus_tag="ACHARQ_000387"
     CDS             37202..37519
                     /locus_tag="ACHARQ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8408840.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37763..39373
                     /locus_tag="ACHARQ_000388"
     CDS             37763..39373
                     /locus_tag="ACHARQ_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8408841.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39530..40378
                     /locus_tag="ACHARQ_000389"
     CDS             39530..40378
                     /locus_tag="ACHARQ_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8408842.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            40609..41088
                     /locus_tag="ACHARQ_000390"
     CDS             40609..41088
                     /locus_tag="ACHARQ_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8408843.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            41121..43349
                     /locus_tag="ACHARQ_000391"
     CDS             41121..43349
                     /locus_tag="ACHARQ_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8408844.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            43478..45121
                     /locus_tag="ACHARQ_000392"
     CDS             43478..45121
                     /locus_tag="ACHARQ_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8408845.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45194..45739)
                     /locus_tag="ACHARQ_000393"
     CDS             complement(45194..45739)
                     /locus_tag="ACHARQ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8408846.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(45762..46247)
                     /locus_tag="ACHARQ_000394"
     CDS             complement(45762..46247)
                     /locus_tag="ACHARQ_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8408847.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(46237..47049)
                     /locus_tag="ACHARQ_000395"
     CDS             complement(46237..47049)
                     /locus_tag="ACHARQ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8408848.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(47188..>48299)
                     /locus_tag="ACHARQ_000396"
     CDS             complement(47188..>48299)
                     /locus_tag="ACHARQ_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8408849.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        15897 a         8765 c         9362 g        14275 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt
     7561 gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa
     7621 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
     7681 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
     7741 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
     7801 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
     7861 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
     7921 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
     7981 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
     8041 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
     8101 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
     8161 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
     8221 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
     8281 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
     8341 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
     8401 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
     8461 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
     8521 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
     8581 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     8641 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     8701 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     8761 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     8821 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     8881 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     8941 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     9001 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     9061 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     9121 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     9181 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     9241 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     9301 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     9361 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     9421 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     9481 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     9541 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     9601 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     9661 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     9721 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     9781 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     9841 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     9901 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     9961 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    10021 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    10081 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    10141 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    10201 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    10261 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    10321 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    10381 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    10441 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    10501 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    10561 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    10621 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    10681 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    10741 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    10801 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    10861 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    10921 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    10981 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
    11041 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    11101 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    11161 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    11221 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    11281 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    11341 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    11401 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    11461 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    11521 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    11581 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    11641 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    11701 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    11761 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    11821 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    11881 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    11941 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    12001 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    12061 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    12121 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    12181 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    12241 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    12301 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    12361 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    12421 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    12481 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    12541 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    12601 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
    12661 ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac
    12721 ttctacgcca aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt
    12781 aagataaatt caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa
    12841 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
    12901 acatttttga aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa
    12961 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
    13021 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
    13081 aaaaaagtat atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa
    13141 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
    13201 gtgacgagcg aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg
    13261 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
    13321 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
    13381 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
    13441 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc
    13501 gggggagatt tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat
    13561 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
    13621 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
    13681 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
    13741 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
    13801 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
    13861 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
    13921 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
    13981 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
    14041 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
    14101 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg
    14161 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
    14221 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
    14281 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
    14341 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
    14401 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
    14461 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
    14521 attatttctg aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat
    14581 ttcacacgga aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
    14641 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
    14701 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
    14761 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
    14821 cgttgaatac aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
    14881 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
    14941 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
    15001 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
    15061 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
    15121 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
    15181 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
    15241 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
    15301 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
    15361 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
    15421 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
    15481 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
    15541 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
    15601 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
    15661 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
    15721 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
    15781 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
    15841 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
    15901 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
    15961 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
    16021 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
    16081 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
    16141 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
    16201 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
    16261 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
    16321 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
    16381 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
    16441 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
    16501 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
    16561 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
    16621 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
    16681 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
    16741 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
    16801 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
    16861 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
    16921 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
    16981 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
    17041 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
    17101 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
    17161 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
    17221 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
    17281 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
    17341 catgatctat ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac
    17401 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
    17461 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
    17521 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
    17581 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
    17641 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
    17701 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
    17761 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
    17821 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
    17881 cgcaaatcaa gtctgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
    17941 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
    18001 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
    18061 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
    18121 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
    18181 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
    18241 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
    18301 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
    18361 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
    18421 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
    18481 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
    18541 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
    18601 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
    18661 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
    18721 gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
    18781 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
    18841 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
    18901 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
    18961 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
    19021 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
    19081 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
    19141 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
    19201 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
    19261 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
    19321 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
    19381 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
    19441 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
    19501 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct
    19561 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
    19621 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
    19681 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
    19741 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
    19801 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
    19861 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
    19921 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
    19981 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
    20041 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
    20101 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
    20161 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
    20221 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
    20281 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
    20341 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
    20401 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
    20461 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
    20521 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
    20581 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta
    20641 ggattgtctc taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct
    20701 ttaattttta aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt
    20761 tataacaaag gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc
    20821 tttgttttta gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc
    20881 cagtagcgaa cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc
    20941 gtctagccag acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc
    21001 agctcgtgcg atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga
    21061 attagatagt gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga
    21121 atacgaagca agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct
    21181 agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt
    21241 gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac
    21301 agattgggat ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg
    21361 caatcaaagt acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca
    21421 agatgattaa gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact
    21481 agacaaaata acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat
    21541 catatctttg ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa
    21601 ttagatgaac tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat
    21661 gaacgaaaag gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct
    21721 atgggacttc caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa
    21781 gtgcaggaaa accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa
    21841 aacttggaaa tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat
    21901 ttatcttgtc ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat
    21961 gaaacactga aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct
    22021 tattttgatt ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc
    22081 tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca
    22141 gtagtcatca aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact
    22201 gctttggcga atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt
    22261 acaggtggga ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg
    22321 ttacagattg gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa
    22381 gaattaacgc agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa
    22441 ttacgcacca tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata
    22501 aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt
    22561 gggaaaataa cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag
    22621 agcttaaaga aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa
    22681 agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc
    22741 acgagctatt gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa
    22801 aaatgctcgg tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa
    22861 cacaccgagc ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc
    22921 cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct
    22981 atggcccagc aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag
    23041 ctgctgctgc agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt
    23101 ctattagaat acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga
    23161 tgccagttgg tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga
    23221 cagtttctat gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg
    23281 cgccatatca agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga
    23341 tagctttttg tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg
    23401 taaaaaaagc tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc
    23461 cactagatgg tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag
    23521 caatgatccg ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac
    23581 ccatttcttg gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt
    23641 ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc
    23701 tattcctttc accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg
    23761 atgcgataag agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg
    23821 ttgtttcttc catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc
    23881 tgactggaca accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag
    23941 gaatccaatt tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat
    24001 ttgaagcaaa tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag
    24061 aagaatgaag aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc
    24121 ttgcggtaca gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa
    24181 agaattagta gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa
    24241 tgaattagtt ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa
    24301 aatctccttt aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc
    24361 agattttgct gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc
    24421 agagactttc aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat
    24481 ccactcactt gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat
    24541 gcgtttagcg gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca
    24601 gtatttaagc gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca
    24661 gaaaatggcc gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt
    24721 caatgaaaac gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat
    24781 cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata
    24841 ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg
    24901 atgtactttc ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa
    24961 ggcttggtat acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg
    25021 ttgcttttga cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt
    25081 tatttatcgt tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg
    25141 ttaccttatc aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt
    25201 gcatttattg gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac
    25261 gctggtttag cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa
    25321 gcttttcgaa tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact
    25381 tcgttagcag caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt
    25441 cggacattcg attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt
    25501 atcgggatcg aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga
    25561 aaaatagaaa tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc
    25621 tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag
    25681 aaaacatgaa gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg
    25741 agcaaggcgg cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata
    25801 cgcttagctg cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag
    25861 tgacaaaagc agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt
    25921 ttgttttacc aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg
    25981 ttttagtgta tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga
    26041 ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct
    26101 tcatggcttc tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat
    26161 attttccaac agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat
    26221 tttctaacga acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg
    26281 agttgcaaga agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc
    26341 agatccgtcg gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag
    26401 aggaaaacaa tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa
    26461 taaaatccaa ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga
    26521 attatttcaa aaaaagcacc atcgaatcgc tgtgatccaa aatccccaag taggacaatt
    26581 caaattattc gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc
    26641 tgcacagatc ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct
    26701 tgttttcgat ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt
    26761 tgattcgaaa tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa
    26821 aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac
    26881 agatgacttt tttagtgatg actatctttt tttccacgca gacggatata ttggattttc
    26941 tgatttcacg atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc
    27001 tatccatcta acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga
    27061 cagtaacgac tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa
    27121 aaaatggatc atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt
    27181 ggctctttac cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat
    27241 gcatcatttg gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta
    27301 ttcaaaagtt caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg
    27361 aatagaaaag aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag
    27421 tggtagaaat cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc
    27481 aaaacgggca gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct
    27541 atgtattaat agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc
    27601 ccgtttacac aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc
    27661 atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac
    27721 atttgttgac gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag
    27781 ctaatgaacc gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg
    27841 aatttccttt tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa
    27901 atcatctgag caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca
    27961 agaggttgta acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc
    28021 tcaaattttt gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc
    28081 atcgagaggt cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt
    28141 cgatacattt atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca
    28201 tcgtttgaca cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct
    28261 tctacataaa ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg
    28321 cgcgattgca atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa
    28381 tactcatata aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc
    28441 agtttctttt taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac
    28501 tctgcaaact catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa
    28561 tcctcttgat ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt
    28621 tccggctgaa aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat
    28681 acatagcaat aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg
    28741 aaaatatttc caaagctttt aaaagtaact ttgctttttt gtatattata ccagcaagcc
    28801 agtcacaata gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg
    28861 ttttgcgaca agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc
    28921 gatagaagag aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc
    28981 agtaaaatgg aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag
    29041 tttagtcgcc atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa
    29101 ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg
    29161 gaagatagca ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt
    29221 gatcgccaca tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc
    29281 tttgttttct tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat
    29341 tgaagccaga atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga
    29401 atcgatcagt gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac
    29461 actcatggtg aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa
    29521 aacttccgat tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat
    29581 cccgttgatt ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat
    29641 atagcgtaga ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa
    29701 gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc
    29761 aaaagcaaga agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt
    29821 tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt
    29881 caatttgatt gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa
    29941 accaataata cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt
    30001 tacctgtttt gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg
    30061 aaacgcattc attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta
    30121 acgtcttagt aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc
    30181 aacatttata agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg
    30241 cgcaggtaat atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca
    30301 cagtagagtg ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga
    30361 ttggattcgt tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat
    30421 ccaaagagga atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg
    30481 acaataaagg aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg
    30541 ccgctgacgc atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt
    30601 cgattgcaat cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat
    30661 agtcatgggc agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa
    30721 tcagtttgat tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg
    30781 tttggaaata acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca
    30841 cgatcccaaa tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt
    30901 taccagtaat tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat
    30961 agttcacagc tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt
    31021 ttagatcgac tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc
    31081 atactgggaa agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat
    31141 acagttggtt caaggaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa
    31201 cgattggttt agttgtttca atatactcat tttttccaaa accatcttct gagacgattt
    31261 tttctacgtt gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc
    31321 tttctagttt attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga
    31381 cactgttgat taataagcca taaccacgta attcatcgat taaccgcaag atatccatat
    31441 cataagtaat cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc
    31501 aaatcagtat ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt
    31561 caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta
    31621 aatataattt gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat
    31681 atttttctga atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt
    31741 ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa
    31801 aatggtttaa accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga
    31861 aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg
    31921 atttttaccg gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact
    31981 tatctaaacg aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca
    32041 agagatctgc ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat
    32101 cagatggaaa gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt
    32161 tatggagcat tgcccgctct tttttataac aattttttac agaaaatggt actattgaag
    32221 tattaataag tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac
    32281 aaatgcaaat tatgagccag acgaactaga ggagacgaat gcggatcagg atacaggaca
    32341 agaatcgtct tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt
    32401 agaagactta caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg
    32461 tttatacaat ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat
    32521 cgatcaattg ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga
    32581 acaagtagaa aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga
    32641 tcggcccacg gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga
    32701 tgaagagtgg gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga
    32761 gctagacgca aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa
    32821 agaaatcaat cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa
    32881 aatcgaggaa gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt
    32941 aggctggctt ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa
    33001 gcaagaagca agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat
    33061 cagacgctat ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa
    33121 ttatcttggc aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg
    33181 aacacgatga agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga
    33241 acacttcatt ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc
    33301 tagaggaaac aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg aatactgcag
    33361 aaaacaccca tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag
    33421 atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta
    33481 tgagaaaaga tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta
    33541 tattgaatga agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt
    33601 atgaattact gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca
    33661 catggaaaac aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct
    33721 tttcctttgt attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa
    33781 acgtatcacc agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc
    33841 gacactggtc agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat
    33901 tgaatcaatt attagcccat gcaagaacac ttggttttac cgttaatcag acattactag
    33961 acaatacgaa gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat
    34021 tggatgatat ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg
    34081 aaactactta tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa
    34141 aagatcaaac agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct
    34201 ttatcaatta cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg
    34261 attaccagat gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata
    34321 attgtcacaa gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag
    34381 ggatttcatt aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt
    34441 gtgtagcagg taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt
    34501 ttttatttat agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta
    34561 ttgcgatcag cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct
    34621 atactgttgt tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc
    34681 tatggttaaa gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg
    34741 tttttgatac gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt
    34801 cgtttgattt gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg
    34861 tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt
    34921 gcaatgagtg tcagtatact cattttgaca agttgcagta caacctatac acaatcggct
    34981 tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat
    35041 ggtcaaaact tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt actgagtatt
    35101 ttgaaacgaa attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac
    35161 tcacaggatg agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact
    35221 actggtgcca aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa
    35281 ctataagttt cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt
    35341 tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt
    35401 gctgctctag gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt
    35461 ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt
    35521 ttgctgttat tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt
    35581 gaaggacgag caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt
    35641 ggggcacttg gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt
    35701 aatccgatca ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta
    35761 cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt
    35821 ttgcgcgaaa gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta
    35881 atcggtttgg tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca
    35941 tttgatgcga tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta
    36001 gcaaaagaat tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa
    36061 atcaagttag cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat
    36121 ttaagtccaa cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat
    36181 gaagcttact cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag
    36241 aagatgtata tatacattga aacagataaa tatgacccaa attattctcg taaataaaac
    36301 gtttattcat gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg
    36361 ctacaattta gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga
    36421 gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg
    36481 ccactttgta taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt
    36541 ggatttgatc ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag
    36601 tttttagttg catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt
    36661 ttctttttag aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa
    36721 gaaaagcgga cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg
    36781 aactacttaa agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat
    36841 tggttctcaa tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg
    36901 attcgagtag atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt
    36961 atcatgcaga tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg
    37021 gtcgtattca tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct
    37081 tcatttgccg gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga
    37141 gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac
    37201 catgactaaa ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc
    37261 gcgcttgttc ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg
    37321 gatgaaatat gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac
    37381 tcatgaacat ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg
    37441 cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt
    37501 tttgctgtct atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt
    37561 gttcaaactg atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc
    37621 gtagggataa aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt
    37681 agagagggat ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga
    37741 aagaatagtt gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac
    37801 ctttgctaat cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat
    37861 gatggggaac aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc
    37921 ttatggattg ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt
    37981 agctgtagcc acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat
    38041 caatatcatg tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt
    38101 actagccgga atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt
    38161 atgcccatct ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa
    38221 aaatgatctg cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt
    38281 tatcttggct ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt
    38341 attgatcagt acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat
    38401 cgtcggacgc agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt
    38461 cgaaagagaa aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt
    38521 tgtcggctca gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct
    38581 tcactttttc cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc
    38641 tgtcaatcca aataaattga cattgatcct gatctccatg gtaggaacag tagctataag
    38701 cagcttgcca atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt
    38761 aggagatagt ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt
    38821 attagctgga ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt
    38881 ccaaatggct ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct
    38941 tcaatctttg aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa
    39001 agtcatcttc caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa
    39061 tggcttagca ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg
    39121 aatagcacct ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat
    39181 tatgcttgca gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc
    39241 aaaaagctat gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt
    39301 attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag
    39361 aagcaaagaa taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat
    39421 gattacgctt gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga
    39481 tatagttata aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc
    39541 agtagttaca gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt
    39601 cgttattcca atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga
    39661 agcggatgaa tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc
    39721 tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat
    39781 tttaagtatc catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa
    39841 agatgacatc agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat
    39901 gggacacatg gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat
    39961 acttgcacat atcgatcgaa tccgagataa tacctacgct tatctgattg tagatgattt
    40021 gaacaattta gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt
    40081 aaaaatcaaa ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg
    40141 ttcaagtaaa aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat
    40201 acaaccgcca gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga
    40261 gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc
    40321 agtcattggt acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt
    40381 ccttttattc aaaaaacata ccaaaaatat tccaacatca tcttggactt tctacatgtt
    40441 actaatgagc gtgtaaaaga acgagatggt gtttttattt ctatcaatga agctcttttt
    40501 ctttgcaatc ctcacttcaa tcctctataa tgtcgcatat agtttagtaa atgagtaaac
    40561 tactaattta cttatttact aaaaataaaa ggatgaggag ttaaaataat ggaaatacca
    40621 acaaatttaa aacataaacc agtaatcgtt gtagaagact atgataaagt tgatggaaga
    40681 aatgcattaa atacggatgc aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt
    40741 ggaaaagtcg atatctcagg caaggtatgg cgacatactg gagaaaaatg gtcacgacag
    40801 tcggaagaat taccaatcac acgtatttta gatttagcga tcttagctgc acaaggaagt
    40861 ttatacttcc aagatgctta tcgttatgaa aaattttatg atccagaaaa tccagtcgta
    40921 gacatcattg gattacaagg gaaccgaatg acggtagaag tagataccaa aaatccaaag
    40981 atcgatgaag acatcatttt gtattacgat acactacaaa aagacggaga actgattggg
    41041 gaacgattcc gtattttaaa acgtctttta gatgagctag gatattaata gacagagatg
    41101 atcaaaaggg gaagagaagc atggagaatt tctcactaac agaaaaccga accttttcac
    41161 ggctagaaaa gcaaatgatc tggaagaaac cgaaaagcca tcggacaagt gcagaagaat
    41221 ggcggatcgc ctccgaaatc aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg
    41281 tacttttgga aggagatgcc gggtcgggga agacacaatt agccaaagca ctatctgctg
    41341 atctgcaact tccctataca aaagtgacct gttttgcaga tatggacaaa tcagatgttt
    41401 tcggagcctt attacctgta gtagattcta ataaggaaca agaccaagaa ttactagaag
    41461 ctatttacca aacagataca cttgaagctg tactgacact gatccagaac cattacggca
    41521 ttgatccttt ttctgctaaa gaaaaattag cagagctagt tcaaaggatc gaagctgatt
    41581 atccagatgc aatccattac agctattacc cttctgaaat cgttcgagcg attgaaaaag
    41641 gatatttatt agagattcaa gagccgactg tcatccgaga tgcctctgtt cttgtggcac
    41701 ttaactcagc gctcgaacca aatgggatgt tgaatttacc gacaggaatc attcgtcgcc
    41761 atccagattg cgtcattgtg atcactacaa atcgaaatta ccaaggaaat cgcccattaa
    41821 atgaatcttt aagagatcgg atgcaacatg cggaaaaaat ggatcttcct gccttagaag
    41881 tcatggcaga acgtgcgatg gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga
    41941 tggcagagat tatccgtttg ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag
    42001 caggaatgcg ttcttatttt tattgggcaa atacctataa gcaaggacaa gatttactcc
    42061 agtcgattta tccaaaagtt ttgtataaat tatccactga ttcagatgaa ttggcaattc
    42121 ttgaacaagc tttgtctgac agtggattgc tactggaact tcaggacata ttgagaaaag
    42181 aaaaatgggg tcatcctcaa tctgagcata caaaaggaag aaccattagc gccgaagaag
    42241 caaatgaacg aaacatcact gaaaatgctg aagaaattct gcgcttagat gaaaagaaat
    42301 cgttgtctgc tgaagaacca aatgagggag aagagaaaca agaagaaaca atagaaaaaa
    42361 acgagattcc tcctcaaatg caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa
    42421 aagaacaaca aacaggagat gattctcaag aaatgtctgc ttctgacatg gaagcacatg
    42481 ataaggaaat caagaagcaa ctgaataaag aagcgcgaca aatcatgaag gaaacgattc
    42541 atgaaaaaga aggtctcatc gttcaccgac cagctttcca aggaaaaacg ccggaagcac
    42601 gtgagctaca catgcaagta atgccaatag tagaatcact tagccggcaa attttagagt
    42661 tactggataa tgaacaaaca gagacgtacc aaaaaggaaa atacgaaggt cagcggttca
    42721 atgcaagcaa agtagcatat ggtgatttga gaaattttga caaaaagaac ccgccacatg
    42781 agcaaccttc tttagcggta gctgtacgga tcgatgaatc tgggtcaatg atacgggatg
    42841 atcggatcga agcggcaaaa aaagcagcga ttgccattgc cgaattttct aagaaagtcg
    42901 atatcccatt gttgatttac ggagatacag cagaccgttc gtcaagagaa aagacttctt
    42961 tgttttctta taaagaattt gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta
    43021 tgaaaccaag gcaaaacaat cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa
    43081 acagacaatc cgcaacaacc aaactgttgc ttaatattag tgacggtcag ccaaaagcgt
    43141 tgccggatta tacaggcaaa aaagcaaaag aagatatcca gtctgttttg aaagaatatg
    43201 aacgtcaagg aatcctattt gtctccgcag ctattggcca agacaaagaa gaaatcaagt
    43261 cgatctacgg tgaaagtcgt tttgtagata ttactgactt gaatgaattt cccaagcaaa
    43321 tgattcaact gattgcccgc tatctatgat aaaataaagc tcaacgaacg cacttagcag
    43381 attcgttgag cttttcgttt ctagttaccg gtaacaagga tacttctaaa atagaatcgg
    43441 ttataatcaa gagggaccgt taaaggagtg aaacgatatg atacaagaac aaaaatggtg
    43501 gaaagaagca gtaggctatc aaatctatcc tgctagtttc aaagatagca atggcgatgg
    43561 tcaaggagac atcaatggga tccgtgaaaa acttaattat ttgaaagatc ttggtatcgg
    43621 ctttatctgg atcaatccga tctatcaatc tccatttgtc gataatggtt atgacgtaag
    43681 tgactatcaa gatatagaag agagattcgg aacgatggaa gattttgatt tattgctaaa
    43741 agaagcacat gaattaggga tcaaagtgat tatggatctt gtcatcaacc atagttcgga
    43801 tcagcatcaa tggtttgaag aatcgaaaaa atctaaagaa agcccgtatc gcgattatta
    43861 catttgggtc gacggggtag atggcaaaga accgaataat tggacatcga ttttcggcgg
    43921 ttctgcttgg gagtattctc atgaaactgg acagtattat ctgcatgttt ttgcaaaaga
    43981 acaaccagac ttaaattggg aaagcgagaa attgaaagaa gaactattca atatgatccg
    44041 atggtggttg gataaaggga tagatggatt tcgtttagat gcgatttctc atgtaaaaaa
    44101 agatgaatat tcagtaaaag ccacagagaa ccctttctca ccctttcaaa atgtctcagg
    44161 aatcgaagaa catttgaccg atttgaaaca tgtttttgaa gagtacgata tcatgactgt
    44221 cggagaagca agtggtgtca ctgcagaaga aggaccacaa tgggtaggga aagacggtta
    44281 tttcgatatg atattcgaat ttgatcatat ccatatctgg cagcaagaaa aagaagggca
    44341 attagatgtc cttaaattga aacttgcatt aagcgcttgg caaacttcat tggatggtat
    44401 cggatggaac gctttatata tggaaaacca tgatgttcct cgtgccgttt cagtgtttgg
    44461 agatactcgt cccgattttt gggctatgtc tgcaaaagca atcgctatga tgtatttctt
    44521 tttgcaaggt acgccattta tttatcaagg acaggaaatc ggaatgacga atatgccctt
    44581 tgaatcgatc gatcaagttg atgcagtaga ttcgaaaaga ctttataaaa ggctgcttgc
    44641 ggaaggtaag acaagagaag aagcattgga tatcattcgt gaaacaacga gagacaacag
    44701 caggacaccc atgcaatgga cttcggaaca atatgctggt ttttctacac atgaaccttg
    44761 gctgatcact aatccaaata ccaaaacgat caatgtagaa caacaagaat atgaaccaga
    44821 gtcggtcctg caattctata agaacatgat ccgcattaga caaaccaata aaggtctgat
    44881 atacggttct tataaagaat atcttcatga acatccacaa ctttatgtct atgaaagata
    44941 tttagaagat gaacaatatc ttattatggt aaacttaaca gaatcacttg ctgactatga
    45001 acttccaaaa gaagcagatc agtcctggac attactcttg tcaaattcct ctagcggaga
    45061 atttgaagct aaaggcatac tagcacctta tgaagctcgg ttatataaaa caaacaagta
    45121 aaacttcctc gttaaagttc attagttgga ctttgcgagg aagtttttca gattgctctg
    45181 tcaattactc atttcaataa ttcgctctag gaccaccgat atatttagga cgagagcgaa
    45241 gggctgtcac gtgagcttgt ccaagggttt tcaaacgagg tctgacaggt acttggacat
    45301 gtttgatatg cataccgatt gatgtgtcac cgatatcgat accggcttgt ccaactacgt
    45361 gttccacttc gacaggatca ttgaactggt caaaagcagc aacagaacaa gcacctccag
    45421 catgtagtgc aggaacaaca gagacaattt cgaaattttt tgcttctgcc actgttcgtt
    45481 ctactactaa tgctcggttc aaatgttcac aaccttggac agctagcgca atttctttag
    45541 gatccaacaa ctcttttaac gtacggatga tccattgacc aacttccgca ctggaatttt
    45601 ttccaatatg tcctccaacg acttcgcttg tgctgcatcc taaaacaaaa agatcacctt
    45661 ttttcaagtg tgcctcagcc aatatgtcat taactatttc agtcaattgg tcttttaatt
    45721 ctttttctga caacttcatt ttctaaaacc ttctttttca attaatattg cttccgtgct
    45781 tgaatacgtt gaagacttgc agttatagga aaggcaagtc ccattccaat cagattttgg
    45841 aagatgtttc ctggtataga agccactcca gccggccaat catataaaat ccaaccggcg
    45901 ataccatagc cgatgatcat aactaagctt cctaataaaa gccctaatcc ccacatacga
    45961 gtagaagatt tgctgaaaga accagcaaca tatccttgta acccgtggat cactaatgaa
    46021 aaaactgccc actgcggata tcctgaaatc aaatcgatca atagaccact ggaagcacca
    46081 acaactaatc cacccgttgg accataaaga aaagcagcgg tatagatccc ggcctcgcat
    46141 aatgtcacaa atccattcgt agccggtaca gggatcaaaa ttgttaatga aagggcaact
    46201 gtcaacgcaa tcagcatagc atagatcgtt aattgcttag ttttcatttc tcattcctcc
    46261 tttttgaata ccgccagacc agacacttcc ggtatgatcg ttcatcagca cgccattaga
    46321 aatacaatgg gcaacaaatt ttttactttc tgctactgct ttggtaaaat ctgcccctaa
    46381 cacaagattc gctgttattg cagaagaaaa ggcacaaccg gccccgttta ctgttgtagt
    46441 gtcgattttt ttatgagaaa atacctcata atgatcaatt gaatagagaa gatccacagc
    46501 ttcatctcca gccatccttg tgccgccttt gatcacaatg ttttttgttc ctgaagcagt
    46561 gagttcttta gcgagatgga tcatttctgt taacgaagag ggaagagctt gaccagaaag
    46621 taaagcagct tctgccaaat taggcgtaat catcgtaaca agtggaaaaa gttcttcgat
    46681 gagctgcgaa atatacaatt gattgatcac agcttcagtt tctttgaatg ccaaaacagg
    46741 gtctaaaatg attgggatat tttgttttgt ttttatgaag tctcgaacaa ccatgattgc
    46801 ttcacttgaa ttcaataacc caattttgat accatctaat gaagtcattt tatcaatcga
    46861 aactaactgt cgtttaagca attccaaaga tatagattcc agcataaatc gctcattaat
    46921 atctagtgcc cctatagaag tcaaggcgct tagtccgaat agaccaaaat tctcaaaagt
    46981 ttttaaatct gtttggattc ctccaccagc aaatggatca gaaccgccaa ttgtcaagat
    47041 tcttttcata acattttcct ccatgaataa gagtataacg aaattttcaa aaaagaaaaa
    47101 cagccaattg gcatattttt atctcatcca atttaaaaaa acaaaaagaa aagaaagacc
    47161 aaaatggatc tttctttcct taaatagtta ggacttttgt gattttaatt ctttctcgat
    47221 ttgaagccgt tgcactttca ttgtagctgt tcgcgggatt gcatcaaaat cacggatgat
    47281 agggacgttc aagcgaggaa gttcatggac ttgctcccac catcgattcc aatccatttc
    47341 tttatcagga gctaaagcaa taatcggttg tggagaatga tttttatccc ttacaatcac
    47401 tacttctgcc aaaaaatcta acgaatctaa taaaaagtct tcaatagcta atgtactatt
    47461 gatcgtttcg atcaaatcca cttgccgatc tttcaaaaat aggtggccat gcttatccat
    47521 aaaaccgtaa tctccgctat cccaccaatc gccataaaca ttttcctgga accgtgcatc
    47581 ttccttgtaa taagtcaaag cccgtccttt agacagtaat tgtatatgac catctgtgtt
    47641 ttcaggtagg acattcccaa ctgaatctgt aatccgagcg cttgtcatgt cttccagtcc
    47701 aacgcccata tctcttgcat cacttgtttt caaactctct agtgtatgag ccttaagaat
    47761 cattggtccg cattcacttt ggccatatac ttgaagaaat accgcatcct gtgccatcga
    47821 cgttcgcaaa aatgtcttca ttgtctggtt gtttatagca tcaaaagtgg agtgatagta
    47881 tcggataccc gaaaaagctt ctggatgctc cttagcagta aaacgccact gaacaaagtt
    47941 attcggatgg gtctctagtg caattggccg gtgagcttcc aacatatgaa caacttttga
    48001 actttgtgcg tttgctaaag gcatcatagg aaatcccatg gacatcaaag aagagatacc
    48061 aatattgaat ctggaatgca ctggagagat gtgaaaagcg attaatttct tttccgaaat
    48121 ttttgtgaaa actgtttttt gccatttcgt ccgccatccc atagaatgag cagaatgaca
    48181 aataagttta gggataccag ttgtaccaga agtatgggtc atataagaaa tttcattttt
    48241 gtctaaaaat gattgagaaa cagacaaagc aggttgttcc attaaataaa gaataggaa
//
LOCUS       DAYYAG010000008        40312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000008 DAYYAG010000000
VERSION     DAYYAG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.008"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1647)
                     /locus_tag="ACHARQ_000397"
     CDS             complement(88..1647)
                     /locus_tag="ACHARQ_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8408850.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1802..4036
                     /gene="pcrA"
                     /locus_tag="ACHARQ_000398"
     CDS             1802..4036
                     /gene="pcrA"
                     /locus_tag="ACHARQ_000398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8408851.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4053..6089
                     /gene="ligA"
                     /locus_tag="ACHARQ_000399"
     CDS             4053..6089
                     /gene="ligA"
                     /locus_tag="ACHARQ_000399"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8408852.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6195..6500
                     /gene="gatC"
                     /locus_tag="ACHARQ_000400"
     CDS             6195..6500
                     /gene="gatC"
                     /locus_tag="ACHARQ_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8408853.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6500..7972
                     /gene="gatA"
                     /locus_tag="ACHARQ_000401"
     CDS             6500..7972
                     /gene="gatA"
                     /locus_tag="ACHARQ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8408854.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7969..9399
                     /gene="gatB"
                     /locus_tag="ACHARQ_000402"
     CDS             7969..9399
                     /gene="gatB"
                     /locus_tag="ACHARQ_000402"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8408855.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9409..10464
                     /locus_tag="ACHARQ_000403"
     CDS             9409..10464
                     /locus_tag="ACHARQ_000403"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8408856.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10575..11951
                     /gene="rlmD"
                     /locus_tag="ACHARQ_000404"
     CDS             10575..11951
                     /gene="rlmD"
                     /locus_tag="ACHARQ_000404"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8408857.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            12160..12996
                     /locus_tag="ACHARQ_000405"
     CDS             12160..12996
                     /locus_tag="ACHARQ_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8408858.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            13145..13366
                     /locus_tag="ACHARQ_000406"
     CDS             13145..13366
                     /locus_tag="ACHARQ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8408859.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            13367..16084
                     /locus_tag="ACHARQ_000407"
     CDS             13367..16084
                     /locus_tag="ACHARQ_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM8408860.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            16077..18032
                     /locus_tag="ACHARQ_000408"
     CDS             16077..18032
                     /locus_tag="ACHARQ_000408"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8408861.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            18022..19158
                     /locus_tag="ACHARQ_000409"
     CDS             18022..19158
                     /locus_tag="ACHARQ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8408862.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(19209..19412)
                     /locus_tag="ACHARQ_000410"
     CDS             complement(19209..19412)
                     /locus_tag="ACHARQ_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8408863.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(19428..21260)
                     /locus_tag="ACHARQ_000411"
     CDS             complement(19428..21260)
                     /locus_tag="ACHARQ_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8408864.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(21263..21709)
                     /locus_tag="ACHARQ_000412"
     CDS             complement(21263..21709)
                     /locus_tag="ACHARQ_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM8408865.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(21702..22652)
                     /locus_tag="ACHARQ_000413"
     CDS             complement(21702..22652)
                     /locus_tag="ACHARQ_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM8408866.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            23047..23664
                     /locus_tag="ACHARQ_000414"
     CDS             23047..23664
                     /locus_tag="ACHARQ_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408867.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            23761..23967
                     /locus_tag="ACHARQ_000415"
     CDS             23761..23967
                     /locus_tag="ACHARQ_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408868.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            23972..24286
                     /locus_tag="ACHARQ_000416"
     CDS             23972..24286
                     /locus_tag="ACHARQ_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM8408869.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            24287..25429
                     /locus_tag="ACHARQ_000417"
     CDS             24287..25429
                     /locus_tag="ACHARQ_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM8408870.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            25431..25973
                     /locus_tag="ACHARQ_000418"
     CDS             25431..25973
                     /locus_tag="ACHARQ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM8408871.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            26034..27971
                     /locus_tag="ACHARQ_000419"
     CDS             26034..27971
                     /locus_tag="ACHARQ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM8408872.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            28182..28952
                     /locus_tag="ACHARQ_000420"
     CDS             28182..28952
                     /locus_tag="ACHARQ_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8408873.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            28945..29289
                     /locus_tag="ACHARQ_000421"
     CDS             28945..29289
                     /locus_tag="ACHARQ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM8408874.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            29282..31525
                     /locus_tag="ACHARQ_000422"
     CDS             29282..31525
                     /locus_tag="ACHARQ_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8408875.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            31663..31872
                     /locus_tag="ACHARQ_000423"
     CDS             31663..31872
                     /locus_tag="ACHARQ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408876.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            31869..32153
                     /locus_tag="ACHARQ_000424"
     CDS             31869..32153
                     /locus_tag="ACHARQ_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8408877.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            32153..33505
                     /locus_tag="ACHARQ_000425"
     CDS             32153..33505
                     /locus_tag="ACHARQ_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM8408878.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            33502..33930
                     /locus_tag="ACHARQ_000426"
     CDS             33502..33930
                     /locus_tag="ACHARQ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM8408879.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            34098..34463
                     /locus_tag="ACHARQ_000427"
     CDS             34098..34463
                     /locus_tag="ACHARQ_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8408880.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            34597..35169
                     /locus_tag="ACHARQ_000428"
     CDS             34597..35169
                     /locus_tag="ACHARQ_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8408881.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            35184..35966
                     /locus_tag="ACHARQ_000429"
     CDS             35184..35966
                     /locus_tag="ACHARQ_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM8408882.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            35966..37201
                     /locus_tag="ACHARQ_000430"
     CDS             35966..37201
                     /locus_tag="ACHARQ_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM8408883.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            37275..37652
                     /locus_tag="ACHARQ_000431"
     CDS             37275..37652
                     /locus_tag="ACHARQ_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM8408884.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            37654..37848
                     /locus_tag="ACHARQ_000432"
     CDS             37654..37848
                     /locus_tag="ACHARQ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408885.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            37832..38008
                     /locus_tag="ACHARQ_000433"
     CDS             37832..38008
                     /locus_tag="ACHARQ_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM8408886.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            38005..38148
                     /locus_tag="ACHARQ_000434"
     CDS             38005..38148
                     /locus_tag="ACHARQ_000434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408887.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            38231..39796
                     /locus_tag="ACHARQ_000435"
     CDS             38231..39796
                     /locus_tag="ACHARQ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8408888.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            39852..>40312
                     /locus_tag="ACHARQ_000436"
     CDS             39852..>40312
                     /locus_tag="ACHARQ_000436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408889.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSS"
BASE COUNT        12967 a         7304 c         9050 g        10991 t
ORIGIN      
        1 tttcccagtc tcttcttttt ttatagaatc atatagaatc aagtagtgta gatgaatagt
       61 tgttgtaaaa tagtttttcc atgttcgtta aaatgattgc ttcaaatttg tgatagaaga
      121 tttagcctcg atttcctgct tgattcttgg caagtttgct tcatcagtca agataaccaa
      181 aatatctcct acattcatga tcgtatcacc gtgagtcaag atttcctgat ttcctctgcg
      241 tatagagatt agcagcatat ttttaggcca attgaagtct cgtaccatta atccgtctaa
      301 agaactctct gccgttactg gaaaatcaaa agttgtcctt gcacctttta gtgaggcggt
      361 ctgagtattt ccaagtagac gttccaataa tgattcgtaa attggattac cgccgagaag
      421 gtcattgacg atataagcaa caagagcagc caatcctaaa ggcatcagat gattaagaga
      481 gccaaccatt tctgtcacta aaataattgc tgtcagcggt gcttttccaa tcgctgtgaa
      541 ataaccagcc atggcaataa cgacaaaatc cttgatgtag attggatcca tcccgaaatc
      601 aaccataatg tttccataaa agctaccgat taaagccccc aatgtcaata tcggaaggaa
      661 aatcccccct ggaagaccac caccgtaaga aatcatggaa aaaacgaatc gaagaacaaa
      721 caaaccgata atcataatga ttggcaagga agcatgtcca aaagctaaaa cgatttgatt
      781 gcctccgcct agagtactag gccataaata accaattggc aaaatcagca agaaaggaac
      841 gaacccgtga aaataagctg gtaaaggcaa tcggttgtaa aattttggca aagccagcaa
      901 gaccttctga taaataaaac ctaagatgcc tagaagtact cctaagatca ctaaggaacc
      961 ataatatttc aacggcaaag aaggaaggtc acctagatat aaaacaggtc tcaagccgaa
     1021 aaagtaaaga gagacgaagt tagcagtgat tgctgaagca aatgatgtca gccaaacaag
     1081 tggagaaaag gagtgatgga tttcttctaa aacaaagagt aatcctgcga taggagcatt
     1141 gaatgctgct gcaagtccgc tacttgcacc gctagaaatt aaaatctttt cttctgaaga
     1201 tggactccga aacaattgtc cagctccttg tccaatggat gcgccaagct gtatagaagg
     1261 tccttctcta cctagaaaaa ggcccgcacc aactgaaagt acaccgccaa tgaacttttt
     1321 ccataaaaca gagaaccagt tcattgataa ttccccgcga agctgtccct ctacttgtgg
     1381 aatcccgctt cccttgatat ccggttcatt tttgaccaga taacccacga aaaatgctgc
     1441 aaggatgcag caaatagcta ggccaattaa gtaaatcggc tgttcccgac tcatttcata
     1501 gatacttcga actaaatcgc ttagcttttc gatagataat cgaaacaggc taacgacgat
     1561 tcctgtcagc agtccaacaa atattccttt tagaataaaa tatatctgag tcttatctaa
     1621 tttcttgatt tttccttttt tttccattca aacgcctcgc tttcttttaa tttagcgcga
     1681 aatcaacgaa ttgacaagga aaaatccata aaaagaaaca aatgattttg aactagctag
     1741 tttaatgaaa gccaagaaaa tgctataatt aacaagattg ataaaagaaa ggatgttgtt
     1801 aatgtcatcc aaagctgaat tattaaacgg gatgaacccc cgtcagaaag aagctgttct
     1861 tcacactgac ggaccattac tgctaatggc aggtgcagga agtggaaaga cgagagtgct
     1921 gacacaccgc attgcctatt tgattgagga aaaagaagtc aatccttgga acatcctagc
     1981 gattactttt accaataagg ctgcaaaaga aatgaaagaa cgtgtcaatg cgattctcgc
     2041 atctggaggc gaagatgtct gggtatcgac ttttcactcg atgtgtgtgc gtattttaag
     2101 aagagatgtt gattttatcg ggtataaccg gaactttaca atcatcgatt cttctgaaca
     2161 gctgaccttg atgaaacgga tactaaaaga actgaacatt gatccgaaaa aatatgatcc
     2221 tcgaagtata ttaggaacaa tctctcaagc aaaaaacagc ctgcagaccc ctcaggattt
     2281 tgcaaaaatg caaggcagtt attacgaaga gattgcggca aaatgttatg ctgcttacca
     2341 aaaagagctt caatataatc aatgtatgga ctttgacgac ctgatcatga atacgattcg
     2401 attatttgaa gaacatcctg attcactcac atattatcaa aacaaattcc attatattca
     2461 tgtagatgag taccaagata caaatcacgc acagtatacg ctagtcaatt tattggcagg
     2521 tagatttagg aacctgtgcg tcgtgggaga tgcagatcaa agtatttacg gctggcgcgg
     2581 tgcagatatg cagaacatct tggattttga aaaagattat ccagatgctg cagtaatttt
     2641 gctagaacaa aactatcgct ccacgaaaaa catccttagc gcagcaaacc aagtcattga
     2701 aaacaatagc aatcgaaaac caaaaaactt gtggacagaa aacaaagaag gaaacaagat
     2761 cacttactac cgtgcagata atgaacgaga cgaaacccgt tttatcgtag atcggatgca
     2821 agaagagatt cgaagcaatc atcggaatta cggcgacttt gccatcttgt accggacgaa
     2881 cgcacaatct cgtgtaatgg aagaaactct gttgaaagca aatattcctt ataaaatggt
     2941 tggagggcac aagttctacg atcgtaaaga aatcaaagat attttagcgt atttgaatgt
     3001 gctggcaaat cctcaagatt caatcagttt tgagcgtatc gttaattcgc ccaaacgagg
     3061 catcggacca ggatctatcg aaaaactacg cagttttgct tctcttcatg aatggccact
     3121 gcttgaagca gcgcaaaatg tggatctggc aaatatcggt ggaaaagcag gacagcaatt
     3181 aggtgcattt ggagagatga tccaagaagt cactcagatg atcccatact tgacagtgac
     3241 agaattgacg aaagaggttt tggatcgtag cggatatcta gaagacttaa aaatccaaaa
     3301 tacattagaa gcacaagcac gcatcgaaaa cttggaggaa tttttaacgg tcacacaaga
     3361 attcgacaaa caattcgaac agcaaaatga agaagacgct gatgcaccag aagaaaaact
     3421 aacggtcttc ttaaatgatc tggcacttgt ttccgatatt gacaatttag aagaagatgc
     3481 ttcccaagtg acattgatga ctttgcatgc agccaaagga ttagaattcc cagttgtatt
     3541 cctgattggt ctagaagagg gggtttttcc tttatctcga gcattgatgg aagaaagcga
     3601 actggaagaa gaacgccgtc tcgcatatgt ggggatcacg cgagcagaag aagcgttgta
     3661 tttaacaaat gctttctcca gaacattata tggacggacg caatataacc gaccttctcg
     3721 ttttgtagaa gaaatcgatc aggagttact ggaaatcgaa ggcatgcgtc ctacgcctaa
     3781 aaaaacacct gtattcgcta aaaaaacagc atatagctat aaacagccag agacagcggt
     3841 tgttccctct aaaagtgcta ctggagggga aaataacagc tggaaacctg gagataaagt
     3901 caaacacaaa aaatggggtc taggaacagt cgtccgagta agcgggacgt cgaaggatct
     3961 agaactagat gtggctttcc caagtcaagg agtaaaacgt cttttggctg cctttgcgcc
     4021 aattgaaaaa gcttagtagt ggagggtaaa tgatggaaca tgaaatgacg attgacgaag
     4081 ctgctaaacg agcagaagaa cttcgtacaa gactgaacca atggtctcgc gaatactacg
     4141 tagaagataa accaacagtg gaagactatg tttatgataa agaatacgca gaattagtag
     4201 caattgagga acaatatcct gatttgatca cgtctgattc tcccactcaa cgagtaggcg
     4261 gaaaagtatt agaaggtttt gaaaaagtca ctcatgatat tccgctttac agtttgaacg
     4321 acgttttcag caaagaagag ctgatagctt ttgatcaacg tgtacaaaaa gcagtgggac
     4381 gtgtagttga ctattgctgt gaactaaaaa tagatggact ttccgtttct ttgcgttatg
     4441 aagatggaaa tttcgtgaga ggtgcgactc gcggggatgg aactgtagga gaaaacatca
     4501 ctgaaaacct aaaaactgtc cggtcggtcc caatcaaatt aaaagaacca atgaacatcg
     4561 aagtaagagg cgaatgtttc atgccaaaac ggtcatttgt ccaattgaac caagacagag
     4621 aagcagaggg aaaagatatc tttgcgaatc ctcggaacgc cgcagctggt tctttgcgtc
     4681 agttagattc taaaatcaca gcgaaaagaa atctggacac ttttttatac acagtagctg
     4741 atttcggtcc tatgcaagca aaaacccagt atgatgcgct agaagagcta gaaaaaattg
     4801 gattccacac gaatcgggaa aaacgtctat gtcattcgat tgatgaagtg tggtcataca
     4861 ttgaagaata tcatgacaaa cgtgtggatt tgccctatga aatcgatgga atcgtcatca
     4921 aagtgaatga attctcgcta caagatcaac ttggttttac tgtcaaagca ccgagatggg
     4981 cagcagctta caagtttccg ccagaagaag ttgaaacgct aatcgaaaac atcgaatgga
     5041 cagtcggtcg aacaggggta gtaacgccaa cagctatcat gacaccagtc cgagtagcag
     5101 gaacgacagt aagccgtgcc agcctgcata acggtgatta tattaagttg aaggatatcc
     5161 gcttgaaaga tacagttctg atttataaag cgggagacat catcccagaa gtttcgcaag
     5221 tcgtgttaga taaacgacct aaagacagcg aagaatacca attgcctaca cattgtccag
     5281 tttgtggaag tgaacttgtc catttggatg aagaagtagc cttacggtgc atcaatccta
     5341 aatgccctgc tcaaatgaaa gaaggtttga accactttgt ttcaagaaat gcgatgaata
     5401 tcgatgggtt ggggccgcgc gtactagaac agatgtatga taaaaaacta gtagcagatg
     5461 ttgctgatct ttacaaattg acagaagaag agttattgac gttggacaaa ataaaagaaa
     5521 aatcagcaaa taatattttg acagcaatcg acaacagcaa agataattcg gtcgagcggt
     5581 tgattttcgg gttaggaatc cgacatgtcg gagcgaaagc agcaaaaatt ttagcagaac
     5641 atttcggtga tctagaaaca ttgagtaaaa gtgactatga gtcgattatt gcgcttgata
     5701 cgatcggtga tatcattgcc gacagtgtag tcacttattt cagtaacgaa gaagtccatg
     5761 aattgatgaa tgaattaaaa caagcaggcg tcaatttcga atacaaaggg cttcgaaatg
     5821 cccaattgca agaagtcgag tctcctttta aagaaaaaac agtagtcctt actggtaaac
     5881 taacacgatt tactcgtgaa gaagcaaaag agacgatcga aaatcttggc gggaaagtga
     5941 ctggtagcgt atcgaaaaaa acggatatcg tggtagcagg agaagatgct ggcagcaagc
     6001 tgaccaaagc acaagaatta ggaatcgaag tttggacaga agatcaaatg gcagatgcat
     6061 tggctaaaag tcgctcagta gaagaatgaa aaatgagttt tttcagacaa atttcaataa
     6121 acaatttcag acacggcact cttgtgttaa aatagagagc aaagttttta actcgtttag
     6181 aaagaagggg tagtatggcg attagtgaag aacaagtgaa acacgttgcc aaattagcaa
     6241 aacttgcgtt cgcagatgaa gaacttgcaa gttttaccga ccaattaggt aaaatcatcg
     6301 atatggttga aatgctggaa gaagtagata cagaaggtgt tccatttacg tcgaatgtgg
     6361 ttgaaacagt aaacgtgatg cgacaagatc gggcgcaagc tggatggaac cgcgatgaat
     6421 tattgaaaaa tgtgccggaa acagaagacg gctttatcaa agtacccgca atcatcgata
     6481 atggggaggc tggcgcataa tgacagaatt acacaatcgt tctctagaag aactgcatca
     6541 tatgcttgta tcgaaagaaa tcactgtaca agatttgaca aaagcaacat ttgaacgaat
     6601 caaagaaaca gagcctgagg tcgaagcatt cattacattg aacgaagaaa aagcattgga
     6661 acaggcaaaa gctttagatg aaaaagggat tgatgaaagc aacgtccttg ctggtattcc
     6721 aattggtatc aaggataata tcgtaacaaa agatatcttg acaaccgctg catctaagat
     6781 cctttacaat tttgaaccaa tttatgatgc aacggtcatg gataaaatct atcaagcaga
     6841 tatgatccct gttggtaagc taaacatgga tgaatttgct atgggtggaa gtacagaaac
     6901 ttcttatttc aagaaaacaa aaaatgcttg ggacccaaca aaagtacctg gtggctcttc
     6961 tggtggttct gcagcagcag ttgcagctgg acaaattcca gtttctttag gtagtgatac
     7021 tgggggaagt atccgccaac cagcagcttt taacggcatc gtagggatga aacctactta
     7081 tggccgtgtc tcacgttttg gtttgattgc ctttgcttca tctcttgacc aaatcggacc
     7141 aatgacccgt acggtgaaag ataatgcatt agcattgaca gctatcagcg gttatgacga
     7201 aaaagacggt acttcttcag gcgtttcagt tcctgatttt gcagagggac tgacaggcga
     7261 catcaaagga atgaagattg ctttacctaa agaatactta ggcgaaggtg tggcccctgg
     7321 cgtaaaagaa gctgtattga aagcagcaga aacattccgt tcattaggtg ctgttgtgga
     7381 agaagtcagc ttgcctcatt caaaatacgg tgtggctgtt tattatatca ttgcctcatc
     7441 agaagcaagt tccaacttgc aacgattcga tggtatccgc tacggttatc gctcagaaaa
     7501 tgtgaaatca ttagatgatg tatatgtcaa ctcacgttca gaaggattcg gtgaagaagt
     7561 aaaacgccgt atcatgttag gtacattctc cttaagtgct ggttattatg acgcttactt
     7621 caaaaaagcg ggacaagtcc gtacattgat caaacgagat tttgaaaaag tctttgcaga
     7681 ttatgacttg atcatcgggc catcttcacc aacagttgca ttcggaatcg gtgagaacat
     7741 taacgatcca atcacaatgt acatgagtga catcttaacg atccctgtca acttggctgg
     7801 acttccaggt atgtctgtac cggcaggttt atcagaagga ttaccagttg gattgcagat
     7861 catcggtaat tattttgatg aaaaaacgat gtatcaagcg gcatatgcgt ttgaacaagc
     7921 aacagaattc cataaacaac aaccagctat tttaggaggg aaagacgaat gaattttgaa
     7981 acagtcattg gactagaagt ccacgtcgag ttaaaaacca actcaaaaat tttctctcca
     8041 gcgccagctc attttggtgc agaaccaaac agcaatacaa acgtgatcga ctggggttat
     8101 ccaggcgtgc tgccagtaat gaataaagct gcgctggaat tcgggatgaa agcagctttg
     8161 gcattaaact gtgaaatctc taaagatact cattttgacc ggaaaaatta cttttaccca
     8221 gataatccaa aagcatatca aatttcccaa tttgatcaac caatcggtca tgacggatgg
     8281 atcgaaatcg aagtagaagg taaaaagaaa aaaatccgga tcgaacgtgt ccatttagaa
     8341 gaagatgctg gaaaaaacat gcatggtatt ggcggttatt cttacgttga tttgaaccga
     8401 caaggcacgc ctttgatcga aatcgtttct gaagctgata tgcgttcacc agaagaagca
     8461 tacgcttatc tagaagcatt gcgatcaatt atccaattta cagaagtcag cgatgtgaaa
     8521 atggaagaag gctctatgcg ctgtgatgcc aatatttctc ttcgccctta tggacaagaa
     8581 gagttcggta cgaaagcaga attgaaaaac ttgaactctt tatctttcgt gaaaaaaggt
     8641 ttagcttttg aagaaaaacg tcaagcaaaa gttcttcttt caggaggaga aatccaacaa
     8701 gaaacacgcc gttttgacga aacaacgaat aaaacagttt tgatgcgtgt caaagaaggg
     8761 tctagtgatt atcgttactt cccagagcct gatatcccga aattcgtgat cgatgacgaa
     8821 tggattcaaa aagtaaaagc tagtttgcct gagatgccag cttctcgtcg cgaacgctat
     8881 atccgtgagt ttggacttcc agagtatgac gcaatggtat tgacacttac aaaagaaatg
     8941 tctgattttt ttgaagctgt tttggaaaat ggtgcagatg ccaagcaagc atctaactgg
     9001 ttgatgggtg aagtttctgc ttacttgaac agtgagaaac tggaattagc tgagacaaaa
     9061 ttgacacctg agaacttagc aggaatgatt cgtttgatcg ccgatggaac gatcagctca
     9121 aaaatcgcca aaaaagtctt ccgtgaattg atcacaaatg gcggagaagc ccaagaagta
     9181 gttgaaaaaa atggttgggt gcaattatct gatccgaaca aactactgcc aatcattaat
     9241 gaaattttgg ataacaacca acaatctgtc gatgatttca aaaatggaaa agatcgtgcg
     9301 gttggcttcc tagtcggtca aatcatgaaa gcaactaaag gacaagcaaa tcctggtgtc
     9361 gtgaataagc tacttaaaga tgaattagcg aaacgataga ggctgaaaat gaaaaaagca
     9421 cgcgtaattt ataaccccac ctcgggaaaa gaattattga aaaaaaattt ggcggatatc
     9481 ctacaggcat tagaagaagc aggatttgaa gccagcgcct atgcaactac accagaacca
     9541 gattcagcca agaatgaagc atgtagagtc gcagaactag gttttgatct agtagtggca
     9601 gctggtggcg atggcacaat caacgaagtg gttaatggaa tcgctccgct tgaacaccgt
     9661 cctaaaatgg ccatcatccc agctggtaca acgaacgact atgctcgagc attaaaaatt
     9721 cctagagata atattaaagc agcagcagaa gtgataaaaa aacaccaaac cgtaaaaatg
     9781 gacatcggcc tttctgataa cagttacttc atcaatatcg ctgcaggtgg ctatttgaca
     9841 gaattgactt atgaagttcc atcagaacta aagagtattt ttggttatct tgcttatctt
     9901 gcgaaaggag cagagatgct ccctcgagta aagccaatca aaatgcgctt gaagtatgat
     9961 gaaggggaat atatcggaaa tgcttcgatg tttttcctag ggctgacgaa ctctgttgga
    10021 ggattcgaac agatcgcgcc agatgccaaa ttggatgatg ggaagttttc tttaatcatt
    10081 gtgaagacag caaatatctt tgagatcttg catattgcag cactcatgct gaatggcggt
    10141 cgacatgtcg atgatccaag aataatctat accaaaacaa gcagtttgtt cgttgaaaca
    10201 gaagatgata tcaatcgacc agtaatgatc aacttagatg gtgaatacgg cggggaagca
    10261 ccgatgcatt tccaaaatct tcatcaacac attgagttct ttgccaatac cgatcagatt
    10321 cctgatgaag caatcacagg tacagatgaa gaagaattag aggttgtcag caaagaattc
    10381 gtaaaagaag tcgaacgttt aacggatgaa gatatcgacg gtgacggaaa aatagcagac
    10441 gaaacaccta atcaaaagaa ttaatcaaaa gccggtgctg aacgtagtaa ggtcagtgcc
    10501 ggttttagtt gtgtaattag ctgatttata gtagagttca gtaggattga aaaataggct
    10561 taaaggagca aaatatgaca gaacagcaaa tagtaaagaa aaacgaacga tatacagtcg
    10621 aaatcgtcga cttgagttat gaaggaatgg gggtagcaaa aatcgatggt tatccattat
    10681 ttatcgaaaa tgcactccct ggagaagaag tagaaatact agtagtaaaa gcaggcaata
    10741 agtttggata tggaaaagtc gaaacattta ttacacaatc acctgatcgt cagcctgtag
    10801 aaaacaatgc attacttcgc ataggtatcg cacctttagc ccatttgaag tatgaacaac
    10861 agcttgtctt caaacaaaaa caagtagagc atgtaatggc gaagattgct aagatgccag
    10921 aagttcctgt acttccaacg gtcggaatgg cagaaccatt cggctaccgt aataaagcac
    10981 agatccctgt tcgccgtatc gatggacagt tagccaccgg tttttacaaa aagaatagcc
    11041 atgatctagt ccctatcgaa gatttttata tccaagatcc tgtcatcgat caagcaatca
    11101 aagtagtcag agatatcttg caacggttcc aagtacgagg atataatgaa gaaaaaaatg
    11161 aaggacagct tcgtcacatc attatccgtc gtggtcatta ttcccatgaa atgatggtcg
    11221 ttttagttac tcgtaaagaa aaattcttta aatccaagga aattgctgaa gctattcatg
    11281 aagaattgcc agaagtggtt tcggtcatcc aaaatatcaa tgaagaaaaa acaaatgtga
    11341 ttttaggaga caaagaaaaa gttttatatg gcagagacta tatcgaagat cagctattag
    11401 gaaaaaccta tcgaatctca gcaaagtcat tttatcaagt aaatactcaa caagcagagt
    11461 atctgtacca aacagccatc gagtttgccg agttagataa aaaagacatt gtgatcgacg
    11521 cttattcagg aataggtacg attggcttgt ctttagcaga cagagtggat aaggtgtatg
    11581 ggatggaagt tattccagaa gcaattgaag atgcacagtt caatgcttta aataataaaa
    11641 tcgaaaatgt tcattatgaa gtcggaaaag cagaaaaagt tatgaaaaaa tggcaagaaa
    11701 aagggatcaa accatctgtg atcgtagttg accctccacg aaaaggatta gatcatcatt
    11761 ttattgaatc agcaatcgaa atgtcaccag aacgaatcgt ctatatctca tgtaatccgg
    11821 caacttttgc aagagatgct aagttatttg cagaagcagg ttatgccgtg aagaaagttc
    11881 agcctgtaga tcttttcccg caaacccatc acatcgagtg tgtaggactg cttacacgtg
    11941 tggaacgttg atatgacaag gattagagcg attcgggatg gtttccggat tgctcttttt
    12001 tcgtgttgaa atgacgattt ttgggtgagc gtagagttga tggaaatgct atggagcttt
    12061 gagttgatag gagttacacc gtagacttga taggttagat gattttgata acggttacgt
    12121 gatataatat attatcgagt tttcaaggga gacaaactta tgaagcaaat ttttatattt
    12181 tcagatacac agaaagataa tatttcttat tttgaacgcg agccagcaat cttggtttca
    12241 atgagtcgta gtgatttgcc gtatttgaag gaacaggatt tttcaaatta tccagctgtt
    12301 tatgttttga ttggtggtaa taagcgttac gtgggtcagg cagcagggca atcaatttca
    12361 cttcgtttat ctcaacactt tctcaaggaa tataaagctt gggttgaatc agttttattc
    12421 tttacaagag cagatgggaa aatgtcaaaa gcagatacag attatcttga aaaacgactt
    12481 atccaagatt tccaagaaaa atctgactat gaaatgatga attcaactgc tggtaatact
    12541 tcgtatattg ataaacttca aaaagcaaaa tcggatcagc tatatgacac agtgtttgaa
    12601 attatagatg aaattgcaaa tatcgatttg tttggtactt ccgaagacag tgaaacccaa
    12661 atttatgaat ttagttcatc tggacaatat gaaattgagt atgatgggaa aaagatatct
    12721 tcgaaatctg ctagaggttt attggttgat tttgtttctc agctatttgc ggatggaaac
    12781 tacaacaatc aactgcaaga attaattgtc gatgagacgc cgtcgttcgc tataatttta
    12841 ggacgtaaac catccatgta taacggaaga ccaaattcag ccagggtagc tgatggagta
    12901 tgggtttatg caaatttcaa caaaaagaat gtcagaatca aaattgataa actagctcgt
    12961 caattgggga ttccggttaa aataaagtgg aattaatatt tgttatctcg cctttagtaa
    13021 atctatgctg aaggctttta ttttagaaaa atagtttgcg ttcgcgaaaa tatttcatgt
    13081 ttcgtataat aatgatagct catagatgat ataattgaag ataccagtag aatagaggtg
    13141 tagtatgtca atttcttata ataaattgtg gaagttattg attgatttga atatgaacaa
    13201 aactcaactt cgtgaaatat ctggcgtcag tcccaatgta atagcaaaac tcggtaaaaa
    13261 tgagttggtt gcaatggagt cattattgaa aattgctgct gccttaaatg ttgatgttag
    13321 tgaaataatt agttcagatg ttatagatgc ggaggatgtt aaataaatgg caaaaaataa
    13381 agttttaaac atcactgaaa ttgaagatgg tgtaaaaaaa atactttcaa ataggggcga
    13441 tgatttcatc actcaatttt tgtccttgta cgatatcccc aaaacgtcga ttacacgtgc
    13501 aaaatcaaaa ttcgataatg gtgaaccatt tgtgattaag aacaaacttc attatgaaga
    13561 aattgaaggc aacgttgtca ttgcgattga tgcgattgct caatcaatca aagagcaaaa
    13621 atctaaacca cgatatatct tggtaaacga ttatttagat attgctgcaa ttgatacgaa
    13681 aactcatgaa actttgaata ttccaattga agagctaccg gctaatgctg actttttctt
    13741 agcgtggaat ggcattgaga aggctgatta tcaagctgaa aatccagctg accgaaaagc
    13801 tgctgaacgt tttgcaaaat tgtatgatgt ggtggctaaa gataatcctg atgccaacga
    13861 gcatgcattt aacttgttct tgattcgagt gctgttcttg ttgtttgcag aagatacagg
    13921 aattatgaat aagggttcct ttacaaatgt tctaaaaaca agaacggctg aagatggttc
    13981 gaattttaac gaagtaatca aagaactctt tgcaatcttg gatattaatg agttagctcg
    14041 caatggcaag gatgagtggc tacttgaatt tccatatgtt aatggaaaac tgtttggtga
    14101 gccacatgtg gatttgacct tttcaaaagt ttcaagagag ctgcttattg aagcagggga
    14161 attattgaat tggaatgaaa tcaatcctga tatccttggc gcaatgattc aatcagttgc
    14221 ttcagcagaa gaccgtcacg ttgccggtat gcattataca agcgtgccga atatcatgaa
    14281 agtcatcaag ccgttattct tagatgattt gacagagtca tttgaaactt taaagcttcg
    14341 cagtgaagaa aacgagctga aggacattac tgaaaaaaca agaaacgata ataagagaac
    14401 aatcatcaat gatctaagtg ctttgttgac gcgaatttct aatattaagt ttttggatcc
    14461 cgccagtggt tctggtaatt ttctgatcat tgcttataag gaaatccgta gacttgaaat
    14521 taagattttg caattgttgg gcgacttaca aaataatgat tccatgccac tatcagtgat
    14581 tcatcttggt aattttaatg ggattgaatt ggatgatttt gcgcatgaag tagcaagact
    14641 ttcattatgg attgctgaac atcaaatgaa taaagagctt gaagaagcaa taccaggtac
    14701 cattgctgag ttgttaccgt taaaagatgc tgggaatata gtgtgtgcca attctcttcg
    14761 aattgattgg aatgaggtgt tgcgactcaa agaaaatcat gaaatatata taatgggaaa
    14821 tccaccgtat ctgggcaaaa gtcttcaaaa tgctgaacaa cgtgaagata tggaaaaact
    14881 tgttggcgat attcgaaaat ataaattgct tgattatatt tttgggtggt tttggaaagc
    14941 taaacaatat attgcaacat ctcatggctc gttcgcattt gttactacta attcaatttg
    15001 tcaaggtgaa caagttagta tcctttggcc gtacctttta aaagatgagg taagaatttc
    15061 atttgcatat cagagcttta agtggaacaa caatgcaaag aataatgccg gtgtgactgt
    15121 tgtcattatt ggtatgacaa ataatgtgaa tattcatcct aagttatatt tagagaatgg
    15181 gtcagtaatg agtgtttcaa atattaatgc atatttagct aatggtgaaa atgtatttgt
    15241 caacgaatca agaaatagta tttcaaattt accctctttg aaatttggca gtatggcaaa
    15301 tgatggtggt aatctagttt tttcagtggc agaatactgg aatttgattc aaaaatatcc
    15361 agatattaaa catttattta agaaatattt aggatcaaca ggattaataa atggagactt
    15421 aagatatact ttatggttga ccgaggaaga tgcaactaga tacaaacaaa attctcttat
    15481 taaagaaaca ataaaaaatg taaaaagtta tagggaaaaa agcaagagaa aagaaactcg
    15541 agcactagct gagaagccat ggtcatttgg tgaagttcgt tatgatgaaa ccagtgagaa
    15601 tagtattcta attcctcgtg tgtcatcaga aaaaagagat tatgttccta tgggtatagt
    15661 tgggagcgat actataattt cagattcggc tatggcaatc tataacgctc caatgtggct
    15721 gcttggtctg ttagaatccc gaatgcatat ggtgtggctg cgctctgtcg gtgggaaatt
    15781 agaaactcgg taccgttatt cagccgggtt agtctataat acttttccga ttcaggattt
    15841 atccgcccag cgtaaaaatg aaatgactcg cgtgatgaca gaaattcttg atttacgaga
    15901 atacgaaggc ggcacgttag ccgatttgta taataaagac agtatgccag aatcattacg
    15961 taagaaacac caggaactgg atgggattgt tgatagagct tatcagcaac gcccgtttga
    16021 atctgacgaa gatcgacttg gaacattact aaaactctat caggagatga caatcaatga
    16081 ctagtaattt atttgaaatt aattttgata aaaatcaatc aaaaacaaca aatgacctcg
    16141 gtatgcgtga aatgcaagaa cgtgtttatg aaaagcgtgc gtcacagtat ttgttggtaa
    16201 aatcaccacc agcttcagga aaatcacgtg cattgatgtt tgttggcttg gataaactcc
    16261 ataatcaagg aattaaaaaa gtggttattg cagttcctga acgctcaatt ggtaaatcat
    16321 ttaaagatac tgaattatcg aagtttggtt tttattggga ttgggaagta aagccaagtg
    16381 acaacttaac tattgggggc ggtgaaaaat caaaggttca acactttgtt aaattcatga
    16441 actcgactga cccaagtgat aatatcctga ttgccacaca tgctacgttg cgatttgctt
    16501 ttgaagagtt ggacgattca ttatttgaca atacgctatt agcgattgat gagttccatc
    16561 atgtgagccg tgatgataaa tctgtgttag gtaatgcctt acgtagcatc atgtcaaact
    16621 ctaatgcgca tatcttagca atgactggtt catatttccg tggcgactct gttcagattc
    16681 tggaaccagc cgacgaagat aaatttgata aggtcactta cacttattat gagcaactgg
    16741 aaggatataa atatctgaaa tcctttgcaa tggggtatag tttctatcgt ggaaaatata
    16801 cagatgcttt ggataaagta attgatgtca acaaaaaatc aattatccat attcctaatg
    16861 ttaattctgg tgagtctact aaagataaat acgcggaagt tgatgaaata cttgatttat
    16921 tgacggacgg tggtaatatc acacagaacg aggatggtat ttatgaaatc gaacgtccag
    16981 atggtaaaaa actattagtg gccgaccttg tgaatgaaga aggacgtgaa aaagtctcag
    17041 catatctagc aagtattacg gatgacatta aagatcttga taagcttgat atcatcattg
    17101 cacttggaat ggcaaaagaa ggcttcgatt ggccatttgc ggaatatgca ctgacaatcg
    17161 gttatcgtaa ctcattaact gaagtgattc aaatcattgg tcgcgtaact cgtgacagcc
    17221 ccaataagac tcatgcacag tttacaaatc ttattgcgca gccagatgca caggatgacg
    17281 aagtattatt cgcggttaac agtattatga aggcaatttc tgcatctcta cttatggaac
    17341 aagtgttggc gccagtttac aagtttaaac caaaagatgg taagccagac aaaccaggtg
    17401 atctttcaat cagaggactg aaacctgctg atacgacgga acgtacaaag aaaatcatcg
    17461 aaaatgatat ggaagatttg aaatctagca ttttacagag ccaagatatt caaaatgcga
    17521 ttgtttcagg tgctgatgcg aagatgatta ataagacgtt gattccacgt gtcatcattg
    17581 atcgttatcc tgatttgacg aacgaagagt tagaaaccgt tcgtcaacat acagttgccg
    17641 atataaatat tgcggctgga cggacaacaa cttcagaaga tggtagccgc gaaattatta
    17701 agatggccga tcgtttcatt aacattgatg aattgaatat tgatttaatc gatcaagtga
    17761 atccgttcca acgtgcgtat gaagtgatta gtcgtgatat tgatgcaccg actcttcggt
    17821 ttattcagga ttatttgtca tcaaataagt atgattttac tgacgaacaa ttggttgcgg
    17881 cctaccaagc tgctaagaag ttccgtgcag aaaatggtcg aaatcctgat cgaaatagta
    17941 aagatgatgg tgaaaaatac ttggcctttg ctttgctacg acttgctgag ataaaacgtg
    18001 aaagagaggc agcgaaagaa gatggcgaat agtttagata gcatctttga agatgacgct
    18061 tttgatgatt taaccaagca agttgaaaaa aagaaagttg taaaacaaga cccagaagtt
    18121 gctaaatttc aggagattat agactttgtg aatgaaaacg gacgagagcc agaaaaaact
    18181 cctgaatggt ctagtgaacg cgcattgtgg gctcgtctta gcggatttcg aggaaaaccc
    18241 gaaaggtctg agaaggttaa gaagtatgac actcttggtt tattggagaa agaattcgaa
    18301 ccggatggag agtttctcgc tgattcagtt gaaatcgatg acgagttgaa tttagatgat
    18361 atcttaggct cagatgatat gattgatgat ttttcagact tgttggatgt ttcccgctat
    18421 cgtagaactg tgaacgctgc agataaaatt ggccgcagaa aacatgcaag tcattttgaa
    18481 aagtataaga gcttgttcga tcaagtgcat gcagatatcg catctggcag acgaaaaatt
    18541 atcccgtttg agcagtatga tattcatgaa aatagattct acatccaaaa cggcgtaatg
    18601 atgtatatcg tttccatttc gaaggatacc tttatcgctg ataatgggaa aacaaatgcc
    18661 agaatgcatg ttgtttatga gaatgggaca gagaataaga atctgttgtt acaatcttta
    18721 gcttcgtctc tatactcgac tgaacgtcat ggaaggatgg tgacagagcc ggtagatgag
    18781 gaaacactgg ctgaaagctt tggagaagag tttacgacag gttatatcta cgtgctgaag
    18841 tcattaagca cgaatccgga tattagcaag tggaataacc tgtataagat tggtttcact
    18901 cgaaatgatg ttgaaagtag aattgccaat gcggaaaacg acgtgaccta cttaaatgca
    18961 ccagtgaagg tcatgctttc tgctgaagta caaaatgtca acgctcagct acttgagcgc
    19021 actctccatc atgtattcca ggacaaacaa gttgtctttc aggatggaaa cttcaaaaaa
    19081 gctactgaat ggtatatggt tccattgaat gatattgaat cgaaaatcaa tgaagtaatc
    19141 gctggattac aaaaataaat atgaaaatgc actgtactca tgaatcgaat acagtgcatt
    19201 tttgatattt aatcgcgaac gatttcaaca atatccgata aatcacaatc aagtgcttca
    19261 cagatgcgaa gtaagatatc tgttgttaca ttttcgcctt tatttagttt tgagagtgtt
    19321 gctgaactga tatcagtcat ttttctgagt tgtgctttat tcagtcctct gtcgattagg
    19381 agtttccaaa gcggattgta gctaaacttc atgtaatcat tcctgttcta gttataaata
    19441 tcgtatagat atccatagcg tccattgttt ttatcttcag taaagacaat ctgatttact
    19501 tgtttatcat catagtttag tgctggcaag ttcgttgtat tctcaacgat gataagttgt
    19561 ccttcgtttt gatgatccat aaagtatttg aaaagtcctt ctttcatatt gtcatcgcca
    19621 tcttgttttt tgcgttcatc taatccaaga agaggggaat cgataatgaa cacagaaggt
    19681 gtgtacttag cgtgattata caagagatgt cgatatgcca ttaccactac tgaattgata
    19741 aacgctgtat atccatgtcc gttttcggtc cattttagat ttccattgat tttgatgtcg
    19801 aaaatatcct tggcgaaaaa tgcgttacga aattcattag gtttataatg catattacgt
    19861 aacatggagt ccagtaactc agacatttct ttgtagaatt ctgcatcaaa tttctcttta
    19921 ggtttataaa ggatacgttc gtcttctggc tcattctcga ttttttcaag atcactttta
    19981 agcttatttt gcattttttc catcgagatt agctgttgtt taatatccct gtaagtttcg
    20041 attttattga ttcgatcctt taacgtctca gctttcggtt ttagttcatc gttgattgct
    20101 gcatcaagcg atttctttct tgttttaagc tcatcaagct gttccattaa ctcttgcagt
    20161 tcaacttcgg tatcatgaga ttcttccgtc aaatctgata attgagagag caatgaatgt
    20221 aattctgacc tagcagtatc gatgtaggtt gtttggacag gtttacttag ttcgccgtta
    20281 cagaatggac agctatgtga agtatcatgt tcattatgat catttaataa tcgctcacca
    20341 tctacgatta gtgttagtcg ttcaatatcc gattgatatt gataatgtaa ctgtttgtac
    20401 ctattcaaaa gaagttgagt ttcatgaaga tactgttcct tttgataaat ctcttttatc
    20461 aagattttgc tgctggctaa cgcatcattg atttgctttt caattgtttc aagttcagcg
    20521 aacattccat cgacaatttg ttgaaaatca tcagagggag cctcaggtaa ttctgattta
    20581 gccacttcgt agttttcata taattcagaa aggcttgaat tgatgtagtt aatcaccgag
    20641 ttccttctaa tttttttatg ttcttttgtt tcgttttctt ccgagttcgc aaaatctgaa
    20701 ccgtatagca gaagtaacaa cgatgataaa aaagctgtgc gttgggttgt ttccccagga
    20761 aaaagaacag aggcgctggt attgactcta tcttgtggaa taagtgtgct aggcgctaat
    20821 gtccgccaag ttaatttttg acgtttgaaa tcctcattct tgataatctg ataatcatca
    20881 tcgatgccta aaagttgtaa ccaaaaatgg ctaattggat ttttcccaga tcgtgcatta
    20941 tatttttcat aatctacata ttcacctgaa ctcgttattt tggataccaa aatggagttg
    21001 ctatcaaatt ctctagttaa tgaatatttg ccattagtcg attgaataat cagttcaact
    21061 ttactgtatc cggttgcatc ggttgaaaag ggagcagcgt cttttttcgc gccatagaga
    21121 aaatcgattg ccgtaacaat gctagttttt ccggtatttg aagggccaga aattatgttt
    21181 agtccattgg tgaacttgac agttgcatct tcacgatctt tgccttttat ggtaacacgt
    21241 tcaatataat aattttgcat tatcattgct ctcctttaaa ttcctttgcg cgttggttta
    21301 tttgtcgcag aagctcaacg tcacttttat cttgaatgta tttaacagat tcgtcagcta
    21361 tttgtaagta tttttctgca tataccgata cgaattgtga tatcaacttt aggccatctt
    21421 ctgataaagc atacttaaaa ccttctttgc tttgcgatgg ttggataaca tgttgcagta
    21481 caagattttt gactgcatct ttaactaatt cacgtcgcgc aataaattca ctgaatctat
    21541 agctgttatc accatttaag ttgtaatcgg aaatttcaaa gtcgcgacca tacgtggtga
    21601 tgaagtctgt gattaaaatt ctatcagcgc tgacaggctc tttaaattca gagagcagta
    21661 acattactcg aaccgaaatt tcaaatggag tattaaacaa actattcatc ttcagtcacc
    21721 catttgatct ttccgtcatt aaccagcatg tggcaaatgc cttttttagc agaattttgc
    21781 agcaatcccg gtataaacga taagattgat ccgtttaatg ttataacaga cgactgctgt
    21841 agaacggctt tgagtctctc aacgccatca tcgtagtctt cttcatagac ttcctcgatg
    21901 ccgtcgtaca tatcttcttt taaagcttca aagtgctcag ttccttcctc ttgtgtaaag
    21961 ttatcacgga ccccacgacg tatgctttca gctttatagt aatttcttcg ctgtctcttg
    22021 aaatccttta gccgcgaatt tggcaggtcg tttgtatgct cgtatacttc gtatccatcg
    22081 gcatctgcat atgctgaaaa aagagctatg acatacgtca tctcctcagc tctaatatca
    22141 aggggaactg gcaaatcgtc aaagaatttt ttcacttcat cattataaga gaaatcacca
    22201 ttagtgttga ttcttaaact tgcgagctct aattcgctaa gcttcggctt ggcctttttt
    22261 gtttttactt ttgtttgaga agcaattgag gtgatttcat caagtaaaag atgggccact
    22321 atttcagaaa cgtctgtatc tttggggatt accacacctt tatcaatcaa ttgttgctta
    22381 aggacggttg gagcatcatc tataagacgt tcataaataa aagtcgtgca tttgtcgatg
    22441 ttgaattttc cagcaacccg aactgcatac tcttgtgtta atggtcgagt accattgtaa
    22501 aatcttcgta ggttatcatc agaaaaaccg aaaatggtgg cctcgtcatc atttgaaaca
    22561 ctatccagta aagcaattaa aaaatcctgt ggcttcacgt actcgcttcc cataaacgga
    22621 taaagcgttt gagtaatttc acttatttcc aaaatacaac ctcctttgtt acgtccgccg
    22681 ttgaccgccg tttgccgccg atgactgacg aaaacaaggg aggccttttt ctataattaa
    22741 aacatagtag tttaactatt taattttatc atttatggtt cataaacact acattatgtt
    22801 tcaagaaagt gaacgaattt atcttaaaag cgaatagtcc tactaataaa atataacaat
    22861 gcctgagttg caatagggca aggttacaaa tactgaaaag aactggttat ccagaaaatt
    22921 tttcagatat tgtatgcctt actttattgc gctctttttt cgtgatgagg gacggtgtac
    22981 gtattggcac ggtccctgtt tgttttcctt gctcaaaagc tctttcaggc aagaaagagg
    23041 gatctaatga gtagatttat gaaaacacac caaaacgacc gcagcactta caagtacgtg
    23101 tttgagcgta ctgatgccaa tggacgtatc caaaaagaag tggttgagat tcgcccaggc
    23161 gaagacggcg tcactgagct taacattcag gagctgcatc gcgctgacga ccgtgcgatt
    23221 tacaactttt ataaaaacgc acgtcctgaa cgcacagaag aagaaaaaga aaagatcaag
    23281 gcttggaaag agaagttccg tgaagacttt aataatgagc acggctatta tccaatggac
    23341 gaatacgtgg atggtgaagc caacgattac ttcaaacgta actacaattt gtcgctcaat
    23401 gaatttgatg atgaatgcga tgagggggac aaccccatcc aatcattggt ttacgatgct
    23461 taccaagata atgaagaaga tcctcgtatc gatcgtttgc gtgaagtcgt gacgcaatta
    23521 ttaccgtacc accaagaaat ctatcacatg ctcgtcactg aaaacatgtc tcagactgaa
    23581 gtggcggaca agcttgacaa gaaaaaacag tcaatcaatc gcacctttgg aatcatcaaa
    23641 gacaagatca aagaattatt ttagaaaaaa gttacggggg agtttacttt ccccgttttc
    23701 cttcgcctgt attgtgtaag ggagtagatc ctaaacaaat aaagatgaag gaggaccatc
    23761 atgaacctaa aacacaaaat ccgagtaaac gtgactgatg acacgggtaa caaagaaact
    23821 gtcctacaag gtggtgtgcg tcatttgcct cgctggtttg cacggtggct gttcggtaag
    23881 ggcaacatgg acaaaattct cgtgctcaca ccaggggaca caatcgaatc agtcgaaatc
    23941 cgtgaagtta acaagaaggg agcgtaatga cgtgagtaaa acaaaactat ttcttcaggt
    24001 cgttgaggac gtccaagcgt tagctgatag cttacaagag ttggctgtgg cgatgaaaac
    24061 caatgaacct gaaccacaag ttgaaacacc agaaccgaca atggaaacaa aatcagaagt
    24121 caaacaaccg acgcttgaag aagtccgtgg tttgttggca cgtaaatctc aagaaggtaa
    24181 gtcaactgaa gtgaaagcac tgattgagaa atatggtgcc agtcgcttga gtgatgtgcc
    24241 gacagaaaac tacgcggctt tggttgcaga tgcggaggga ttgtagatgc caacacaaca
    24301 cgcagtccta tccgcatctt caagtaatcg gtggattcac tgtccgccaa gtgttcgctt
    24361 gtcagagcac tttgaaaaca agtcgagccc gtatgcccaa caaggcactg atgctcatac
    24421 cttatgtgag tacaaactga ataagttcat tggtaatgac gtcacagacc caacatctac
    24481 gcttgaattc tacgacgagg agatggagca gtgtgctgaa agttatgcga ccttcgtcat
    24541 ggaagaagtc gctaaggcga aacagacaac agttgactct attgtaatag tagaacagcg
    24601 gctcgacttc tcaaggtacg tcccagacgg ctttgggacc ggcgactgtc tcgtcattgc
    24661 ggatggaacc ctatctgtta ttgacatgaa atacggtcta ggaattttgg tagatgcgta
    24721 tcagaaccca cagatgatgt gttatgcctt gggagcgttg gagcttttcg atgttattta
    24781 tgacattcaa gaagtcaaga tgaccatttt ccagccaagg cgtgagaatg tgagtacgta
    24841 cacactgctg aaagaagaat tacttcagtg ggcaaaagat gtgttgtcgc ctgccgcaga
    24901 tttagctttc aaaggtgagg gtgagtacca atgcgggaaa tggtgtggat tctgtccggc
    24961 gaagaatagc tgtcgtgcac gagcagacca taacttgaaa ctggcgcaat atgagttcaa
    25021 accaccagag cttcttagtg atgatgaaat cgaagaaatc atcggtaaag tggatgacct
    25081 ggtttcttgg agtaacgata tcaaggactg tgctttgaaa cttgcactcg gtggcaaaca
    25141 atggactcat cacaaattag tcgagggtcg ctctactcgt aagtattcta atgacaatga
    25201 tgtcgctgcg gcggtcatca aggctggata cgacccatat gataaaaagt tacttggtgt
    25261 cacagcaatg acgaaggcac tcggtaaaga gaaattcaat gaaatcttgg gcgaatacat
    25321 catcaaacca aaaggcaagc tgacgctagt cgatggttca gataaacgac aagcggtcac
    25381 agtaaacaac ttaaacgaag aatttaaacc attaacggag gaacaataat atgtctaaaa
    25441 caagcaaagt aatcactggt acaaacacac gtctctcata cttccacggt tgggaaccag
    25501 catccatcaa cgggggcgca gagaaatatt caacagcagt cttgattcca aaatcagata
    25561 agagaacgat tgataccatt gaaaaagcaa tcgatgcagc aatcgaagag ggacttgcga
    25621 aattcggtgg gaagaaacca aacaaagcag aaatcaagct gccattacgt gatggggata
    25681 tcgaacgcga cgatgaagca taccaaggcc attacttcat taatgcgaac tcaaccactg
    25741 caccacaaat cgtggatcag cacgttcaac caatcttgga tcgttcacaa gtgtatagtg
    25801 gttgctacgc tcgtgtgtcg attaatttct atgcctttaa cgcaaatgga aatcgcgggg
    25861 tagcggcagg gcttgggaat atccagtttg tacgtgacgg cgaaccactc ggctcacaag
    25921 tatctgcaag cgatgagttt gaaaccttgg cggatgatga tttcttggct taatcattaa
    25981 aacaaaaacg gccatggcag aagtgctgtc gcctgtttga ttggaggaag aaaatgaaac
    26041 atttgtcatt ggatttggaa acgtatagta gtgtcgattt atcgaaatgt ggtgtctaca
    26101 aatattcaga gtcccccgat tttgaaatac tgctatttgc atacagtgta gatggtggtc
    26161 ccgtcgaagt ggttgatctg aagatggacg aaactatccc ggctgaaata ttaacttcgc
    26221 tatcagatga aacagtgatt aaacacgcct tcaatgcgag ttttgaacgt gtgtgtttgt
    26281 cacgctttct ggggttggat ggctttttga atccacgagg gtggcaatgc acaatggtgt
    26341 ggggtgcgac attaggtttg ccgttgtcac ttgtaggagt tggtgctgtt cttggtttgg
    26401 ataaacaaaa gctcacagaa ggaaaagacc tcatccgata cttttgcgtg ccatgcaatc
    26461 cgaccaaagt taatggtgga cgcacgagga acttaccaca ccatgcgcca actaaatggt
    26521 cagcatttaa gacatacaat agtcgtgatg tggaagttga aatagctatc caaggaaaac
    26581 tttccaattt tccagtgctt gattctgttt ggaaagaata ctggctggat caggaaatca
    26641 acgaccgagg aattgagatt gataaggact ttgtggaagc tgccgttcag ttggatgaat
    26701 tatcaaaagc tggattgaat caacaattgc aagaattaac tggactagaa aatccaaaca
    26761 gcgtgcttca aatgcgggga tggttactag accacggact tgaaaccgag tcactcggta
    26821 aaaaggcagt ggccgaacta ctcgaacaac ctattgatga aaatttaaag caagtcctgg
    26881 tcttacgtca gcaactcgcc aagtcatccg tgaaaaagta tcaagccatg caaaactttg
    26941 cgtgttctga taatcgttct cgtggtttat tccaattcta tggagccaac cgtaccggac
    27001 gctttgcagg acgtggcatc cagcttcaaa acttgcctca gaaccatatg tcagatttat
    27061 ctgaagcgcg tagtttagta aaacaaagca attttgaaat gcttaacatt ctctacggca
    27121 atgtcccgaa cgtactctca gaactcctcc ggacagcgtt tgtaccacgt gaagggatga
    27181 aatttatcgt atctgacttc tcagcgattg aagcacgtgt gattgcttgg ctgtcgggtg
    27241 agaagtggcg tcaagaagta ttcaaaaatg gtggcgatat ttactgtgcg tctgccagtc
    27301 agatgttcgg tgtgccagtt gaaaagcatg gcgtgaatgg gcatctacgt caaaaaggga
    27361 aaattgctga gcttgctcta gggtacggag gatcggtggg tgcattgaaa gcaatggatg
    27421 ctcttgaaat gggactaact gaggatgaat tacagccgct tgttaattct tggcgagact
    27481 caaacattaa cataacggag ctttggtggg ctgttgataa ggcagtaaag acttgtgtga
    27541 aagaacgacg gagcacagcg acgcatggca tcaagtttga atacaagtcg ggttttctgt
    27601 tcatccaact cttgtcagga cgtaaattag tttatgtaaa accaaagatt ggtgagaaca
    27661 aattcggcgg tgaagctgtt acctatgaag gtgtcggtgc taccaagaaa tgggaacgtc
    27721 ttgaaagcta tggtcccaag tttgtcgaaa acatcgtgca ggcaatagct agagacattc
    27781 tctgtttcgc aatggagaat cttcgtaagt atcgcattgt cgggcacgtc cacgacgaag
    27841 tgattatcga agtgccaatg gaatcgaagg ttgaagacat ctgtcagttg atgagtcaaa
    27901 cgcctaagtg ggctgaaggc ttgctactga acgctgatgg atacgagtgt gaattctatc
    27961 aaaaggatta gtcaatgtgg agtcagaaat ggctccatat ttttttcaaa tttttttatc
    28021 aaaaataagg ttcgaattgg gttctttctt cgcttataga cgaaggcaaa aaaatcaaaa
    28081 ataaattcga gtcagagttt actttcgacc attttcttcg cctgtaagtt agaaggggca
    28141 aaatgcctct cctgaaaatt ctattacagg aggttcacaa gatgaacgaa ttgaaagttt
    28201 ttgaaaatgc ggagtttggc tctgtacgca cggtgatgat tggcgatgtc cctcactttg
    28261 ttggtaaaga tgtagcatca attcttggct acacaaatcc acaaaaagca ttacgagatc
    28321 atgttgatga ggaagataag acggtgaacg aatcgttcag cgttaatggg acaatggcta
    28381 tccttatcaa cgaaagtgga ttgtactcac tcatcatttc tagcaagatg ccgaacgcga
    28441 agaaattcaa gcgttgggtg acaaatgagg tcttgccgtc tatccgccaa cacggtgtgt
    28501 acgcgacaga agatttcatc acaaagtcaa tcgaagatcc agcatgggcc atttcagtgt
    28561 tgaagcagct acaagagaaa aaagaaatgg tggcgatgca acaacaaatg attttggaga
    28621 tgaaaccaaa agttagctac tacgatttga ttcttcaaaa caattctgtg atgtcgattt
    28681 ctaaaatctc gaaggactac ggtatgtcac cacaggcaat gaacaagttg cttcatgaac
    28741 tgggaattca gtacaagcaa ggcaagatgt ggttgcttta tcagaagtat gccaatcaag
    28801 gctacacaca atcaaaaact catgcgatag atgcagagcg cagcgtgatg catacctact
    28861 ggacgcagaa aggtcgtttg ttcttgtacg acttgctgaa atcgaagaag aacatcttac
    28921 cagtgattga gcgaggtgat aataatgcct agacgtaaag attacagaac atcagtctat
    28981 gtctgctcgc cgttctcagg aaatgttgtc gtcaaccata aaaacactaa gcgttactgc
    29041 cgtattgctc ttgaccgtgg ctacatgcca gttgcggcac atctgatgta tccacaattc
    29101 atggacgacg caacggagcg tgatgtggca atgcacatgt cacttgttct catgggcaaa
    29161 tgtgaggaag tctgggtggt gggtgacacc atctcccaag gcatggcagt tgaaattgaa
    29221 caagcggagt attggaagaa acatatcagg tacttcgatg atgagatgaa ggaggtctca
    29281 aatgcttaag atgacgttat tcacatccaa tcaggtgggt aattccaaga atggattgtt
    29341 cccaaccaag caagtggtga cagataaaga aacacttgtt gccacggtca agctggatca
    29401 tgtgatggct gagtacaaag gcaactatcg caaaggtgac aacttcatcc agtctgatgt
    29461 gattccactt gattgcgaca atgaccactc agacaatcca gatgattggg tagccccttt
    29521 cgaagtggcg atgcagtttc ctgatgtgac gtttgcggtc agctattcac gtaacaacat
    29581 gaaagacaaa ggtggcaaat cagcacgtcc acgttttcac gtatatttcc cgattcctgc
    29641 cacaactgat aaagatgaat atgtggaact caagaagcag attcaaagtg agttcccata
    29701 ctttgacaac aatgcgatgg atgcggcacg tttgcttttc ggaacaccaa actctgaagt
    29761 tgaaatctat gaaggtagta agttgatcac tgattttctc gaagatgatc tgtttgctga
    29821 ttgggataat gcgcagtcag agattagcga aggcagccgt aattcaagca tgagtcacat
    29881 cgcaggaaag ctcatcaaac gctacggtgc aacggaagat acgtatcaca agtatctcga
    29941 acaatcccaa aaatgtaatc cgccactgcc ggaggaggag cttaaagcta tctggaatag
    30001 cgcggtcaac tttgggaaga aagtgaaaca acaagaaggc tacattcctc cagagcagta
    30061 caactctgat ttatcacttg agccaaccga ctactcggac ttaggtcaag caacggtctt
    30121 agctcgcgag tatgaatgta agcttcatta ctcaccgtca actggttacc ttgtctacaa
    30181 cggcagctat tgggaagagt cgaaaccaaa agcgcaaggt gtgatccacg cattgacaga
    30241 acgtcaactg gaagaatctg aaaccgaaat tgagaagcgt accaaggaaa tggttagtaa
    30301 tggcgcgttc gatgtacttg ctagtgttgg tccgaaaaag gcagtcacca tgttcaacac
    30361 agcgcaacgt catagttttg atttgtacca acacgcacaa gagtataaga agtttgctgt
    30421 caaacgccgc gacagcaagt atctctcgtc agcattaacc gaggcaaagc ccatgcttga
    30481 aatcgagcaa cggttactag atgtgaacga gttcttgctg aacacaccaa cagcaacctt
    30541 tgatttgcga acaggcaaat cacaagacca caactcagaa gattacatca cgaagcaaac
    30601 ggaatgtgcc ccgagtgacg ccaatcagca gatttggttg gacgctctga acaccatttt
    30661 cgtaggtgac caagagttga ttgattatgt ccagatgatt gtaggacttg cagctattgg
    30721 taaagtttac gttgaagcgc ttatcatctc gtatggtgaa gggcgtaacg ggaagtccac
    30781 gttctggaat gtcatcagcc gtgtgctcgg taattactct ggcagcatct cagctgatat
    30841 tcttacaagt caaatccgac gcaatgtgaa acctgaactt gcggaagcga aggggaaacg
    30901 cctactcatc gcagccgagc ttgaagaggg gatgcgactc aacacatcta acattaagca
    30961 gctctgctcg acggatgaaa ttgcggctga gaagaaatac aaagacccat tcaagtatgt
    31021 cccaacgcat actttggtgc tctataccaa ccacctccca aaagttggag cgattgataa
    31081 aggaacctgg cgtcgtttaa ttgtgattcc attccttgct acgattgacg gcaataagga
    31141 catcaagaat tatgcggact acctttttga aaatgccggc ggagccattt tgcaatggat
    31201 ccttgacggt gcgaaaaaag tcatcgccgc agactttcat ttaacggttc cacaagttgt
    31261 cgctaatgca atttctgaat ataagacggc gaatgattgg cttggccatt tcttagacga
    31321 gtgctgcgag actgctgttg actttgaaga aaaatctggt gagttgtatg cagagtatcg
    31381 tgcgttttgt acgcgaactg gtgagtacat tcgtagttca gccgattttt acacggctct
    31441 ggaggcgggc ggatttaagc gccggcgaac taataaagga aacaagattg ttggtttgca
    31501 actcaaatcc gagttcttgg aatagttgat tttaaaggaa agtgtaacct gtgtaggtct
    31561 tatatagaac ttttcttaag ggataaaaaa ttaccaccta tataaagtta tgtattgcgt
    31621 attcacgggt tacaccatac ttgcagattg gagaatatct aaatgaaatt taaacagtgg
    31681 ttacaaattg gaatcggtaa acgtgattta cgatttgatg agttttacga gtttgccatg
    31741 tcggatgtca cgtatccttg gaagaaatca ttcgataagc aagtcgcaca tctgatgatt
    31801 cactgcgcag aaaaggaata cttggaaatc ttgcgtgatg cgtatttcga gtatttgacg
    31861 gtgtggctat gagagaaaag caggttgagc aagcactagt gaaagccgtc aaaagggtag
    31921 gtggtatttg cccgaagttt acatcaccgg ggcttgctgg tgtgcctgac cgattggtac
    31981 ttatgccaaa tgggaaactt ggatttgtcg aagtgaaggc gccagggaag aagccacggt
    32041 ctttacagct ttttcgtatg aaacaactga cagatttagg gtttcagtgt ttcgtgcttg
    32101 atgagattga tcagatacct gagttactag aaagaatagg aggtgatgtc tgatgaagtt
    32161 cgtaccacat aactaccaga aatatgcgat tgattttatc aaagatcata aaactgccgc
    32221 ccttctattg gacatgggct tgggtaaaac ggtgacaacg ctgacagcta ttaaagactt
    32281 gatgcatgat gactttacaa tacaacgtgt cttaattatt gcaccgttgc gcgtgacgca
    32341 atcaacatgg ccaactgaaa ttcaaaagtg ggatcatttg aaagatttat cgtattccgt
    32401 ggttcttgga acaccaagtc agcgaatggc agcattacaa aataatgcca acatctatct
    32461 gatcaatcgt gagaacttgg actggctgat taacaaatcg ggttttacgt ttgattttga
    32521 tatggtggtg attgacgaac tgtcgtcctt caaaaactta aaagctaaac ggttcactag
    32581 ctttatgaaa gtcagacaca aggttgaccg aattgttgga ttgaccggaa caccttcaag
    32641 taatggtttg atggatttgt ttgcggagtt caaggttctg gatatgggcg agagattggg
    32701 ctattatatt tcacgttatc gagataaata ctttctacct gacaagcaaa atggcatgca
    32761 gatttattct tggaaaccaa gggaagatgc agaacaagaa atctacgaca agatttcaga
    32821 tatcacgatt tcaatgaagt cagtcgattt cttggatatg ccagagcttg tgatgaatga
    32881 agtgccagtt gatatgggac tcaaagaaaa gcaaaagtac gatgaaatta aggcagactt
    32941 ggtgctacaa ttgaaagaag ccaccattga tgcagccaat acagcggtgc tttcgaataa
    33001 actgctacaa atggcgaatg gagcaatcta tgatgagttt aatgtgagtc atcacatcca
    33061 tgaccagaag ttagatgctt tggaagattt gattgaaggt acaaatggca aaccgattct
    33121 gattgcctat tggttccagc atgatctgga gcgtatcaag gaacgattca acgttcgaca
    33181 gatcaaaacg tctcaggaca ttattgattg gaacgaaggt agcatcccca tcgctgtcat
    33241 tcatccggct agtgcaggac atggattgaa tttacaagct ggtggttcaa cacttgtatg
    33301 gtttggtctt acgtggagtt tggaacttta ccaacaaacc aatgcaaggc tgtggcgaca
    33361 agggcaaagt gatactgtgg tcattcatca cattattgcg aaagatacga ttgatgaaga
    33421 tgtgatgttg gctttgaaac gaaaagataa aacacaatca tgtttgattg atgcagtgaa
    33481 agctagattg gagggaagca agtgacacca aaagaatact tgaaacaagc gtaccgtttg
    33541 aataaacgaa ttgacgaccg gattaaagag aaggcggagc tctatcaact ggcggtgaca
    33601 gtttcttcca caacattcga agaaaagatt tcaagtggca cacggaatac tgaggcacca
    33661 tttgttcgta accttgagaa gtgttaccaa ttggaagctg aaattgatgc agagattgat
    33721 aagtttgtag acctcaaacg acaaatgcgt ggcgtgattg atgaactcac aaacgtggac
    33781 gaaaagattg tgttaatcaa tcgatacctg ttggaagaga gctgggacaa gattgctgaa
    33841 gatcttcacg ctgacgtgcg gacagtcttt cgttggcata gcaaagctct tgagaacttg
    33901 aagctaccag atgaacccat agtcatttga aatgtgtcag tgaatgtcag tagcctttag
    33961 tgatatcatt aagatgtcaa aaaagacaaa cgatactcaa gcaataccag caatagaagt
    34021 atcgaacaaa tactcgaagc ctttgtggga aaaatccttc aagggctttt cttttgcccg
    34081 aaaggatgtg accccacttg ccaatgaaac cagctaaccc ctgtaagttc ccaggttgtc
    34141 cgaaccgtac gcatgatact tattgtgatg aacataaaac acttgcgagg aaacgttacg
    34201 agaaatacga gcgtgatccg gcaatcaaca aacgttacgg cagagcgtgg aagaaaatac
    34261 gggcaaggta cgtggcagca catcccctat gtgagatgtg tctgaaggaa ggacgcttta
    34321 cttcggttga gattgtgcat cacatcaagg aattaagtga tggtggtacg catgatgaaa
    34381 ataatttgat gagtgtctgc aaatcatgtc actcgaaaat tcacatgact cgacgaaaca
    34441 caaaagataa aacaatcatg tgaaaaataa caaggggggg aggggcggta attatcccta
    34501 aacccttcta aactgtacag cgcgctaggc ccttacgcga aaaaagttca gttcaaacgg
    34561 gggatagccc caaacaagaa aaagaggtga taaattttgg caaaagatgg aacacaacgc
    34621 ggtggcagac gacctcgttc aggggaacgc tcacagccac tcgttgataa agttgctgct
    34681 ggaaagaaag catcgcggat ggaatttgac ttaccggatg ttgaattatt aattggtgaa
    34741 gatattggtg agggtgtaga gcttgagggc aatgattatc ctgaaccaag tgcttacctt
    34801 tctgcaaaac agaaggatgg cactgtgcta ggcgctgatg aaatttataa ctcaacgttc
    34861 caatggttga aagaccgcgg atgcgaaacg ttgatagccc cacgaatggt tgagtcctac
    34921 gctcagaact ttgcgcgttt tgttcaatgt gaaaatgctc tgagtcaata tggtttgctc
    34981 ggaaaacacc cgactaccaa ggcagtcatg gcctccccat ttgtggcgat gtcgcagaac
    35041 ttccaaaagc aagcaaatgt gacgtggtat gagattcaac agattgtaaa agagaatgca
    35101 tcagtggaat acacaggaga tccgaatacg gatgcgatgg aacgattgtt acgttctaag
    35161 aatggttgaa aggaacagaa tttatgatag agaaggttaa tccgcaacat ccagataaag
    35221 ttgcagatcg tattgccgga gccattgtgg acttggcata cgcagaagaa aataatccaa
    35281 agatagcagt tgaggtttta attggtcatg gcatctgtca tgtgattatt gaaacttcaa
    35341 ctgctttaaa tttgaatgaa atcagccaag cgattaatcg tatcgcaggc aaaattaaaa
    35401 aagatgtggt gattgtgccc caagataaag agttggctga caatcaagcg actgaaattc
    35461 gttgtggcga taatggaatc tttcgtggga tgccattgac tgatgaacag ttacagctct
    35521 caaaaattgc gcgtgactta tatcaggacc atccatttga tggaaagtac attcttgatg
    35581 gtgaacgctt gataatttgt cagagccacg caaagacaaa tgagttaaaa caaaaatatc
    35641 caaacgctac agtcaaccct ctaggtgatt ggactggcgg cccggatgtg gatacaggtg
    35701 cgactaatcg caagcttggt tccgatatgg cagacggagt aacaggtggc ggtatccatg
    35761 ggaaagactt atcaaaagct gatgtgtcag tgaacatcta cgcattcttg aaagctcaag
    35821 aaactggtat gcccgttgag ttaagctgtg cgattggtga cactgagatt gatggcgtgc
    35881 cttattctga aatcgtggaa gttgcgcgtg attatgtgaa tgagcttggc gggtttgaga
    35941 aactagcaga atgggggttg ttctaatgaa cacaacagaa cgttttgaga aagttgatat
    36001 tgataagctg gtcccttatg ccagaaatgc ccgtacccac agtaaggatc aagtgaaaca
    36061 gattcaagct tctatgcgtg agtttgggtt cataaatccg gtgattgttg ataaggatta
    36121 taacttgatt gcaggtcacg gacgtattct tgcggctaaa gaagaaggta tcaagcaggt
    36181 accgtgcgtg tttgtggaac acctaaccga ggcacaaaag cgtgcgtaca tacttgctga
    36241 taaccgacta gcaatgaacg ccggttggga tgatgaaatg ttggcggttg agttatcaga
    36301 cctgcaaggt gaagagtttg atttggattt gcttggtttt gaaactgatg aacttgataa
    36361 attactcaac ggtaatgatg atgcccaaga tgatgacttt gatgtagatg aggaacttga
    36421 aaagccagcc ttcagtaaaa caggggactt gtggttgctt ggtcgtcacc gtttagtttg
    36481 tggtgactca acaaaacctg aaacttatga gaaactcatg gacggcaaac tagcaaatct
    36541 tgtggtgaca gatccacctt atggggtctc atatcaaggt aaagcgggaa cgattaaaaa
    36601 tgataatctg aaggacgagg cattttacaa cttcttgtat gatgcctttt cttgtatgga
    36661 aagtgtgatg gccaacgacg ccagtattta tgttttccat gcggatacgg aaggcttgaa
    36721 cttcagaaaa gcattcgtgg atgcagggtt ctatttatca ggtacgtgca tctggaagaa
    36781 acagagtcta gtgcttggtc gttcgcctta ccaatggcag catgagccgg tgctctatgg
    36841 ctggaagaag aaaggcaaac atcaatggta caccggacgt aaggaatcga ccatctggga
    36901 atttgataaa ccaaagaaga atggtgacca cccaacgatg aaaccaattc cgctgttggc
    36961 atatccgatt accaattcgt cactcagcaa ctgcatcgtg ttggatagtt ttggtggcag
    37021 cggatcaact ttgattgctt gcgaacagac cgaccggatt tgccatacga tcgaactgga
    37081 tgaaaagttc tgtgatgtga ttgttaagcg ttatatcgag caggctggaa gcgatgcaga
    37141 cgtttcagtg attcgagatg gaaagactat cgcatttaat gacctagaaa atcctgaata
    37201 aataacgtat cacttgctat aaaatccctt tagagtgata tatagactac caaaagaata
    37261 cggaagacaa agttatgttt ccaactcatg aacaaatcga gggtatgaaa aaacgttacc
    37321 ctgaaggtag tcgtgtagaa ctcatcaaga tgaacgaccc acatgcccca gcgaagggca
    37381 cacaaggcac agtaaccggt gttgacgaca ttggttcaat ccttatccaa tgggacaacg
    37441 gttcaacact gaacgcggta tacggcgaag atgtggtgca catcatcaaa ccagacttcg
    37501 aattggtctt tcaaaacggt agaagagaag gatttgaaac cttcaatgat gcttggcaat
    37561 acgtgacgga catgatattg aaccacgatt tggtctgggt tgaccttgag tcaaaaacag
    37621 aagataccat ctacatcaga aaggggcttt aagatgaaag tgagtaaacg taagatttac
    37681 aacatcgcaa agaattacat cgttcagttg cctgaacgtg gcgacctgaa ggcccacaac
    37741 agcgaccgtg aagacttctt ggacattgcg gtttggtgtt tggaagatgc cttaattgcg
    37801 gcctatgagg caggcagaaa ggacggtcaa aatgaacgca aagattaagc aacaaatcat
    37861 tgagattgca gaaagtggtc tgacaaatat gtttgatgcg attgcagtgc agcgaattgc
    37921 acatgaccgg cacttttacg agttggtgat tttcattgaa gaacacaaag atgagtacag
    37981 ccgctttatc ttggagggtg ataaatgatg gattatcaaa tttacatgcc agataatgaa
    38041 gatggcattg aagaaggata ctacgattgg caagaactgg tgcaattgct gcgggataac
    38101 aagaacaacc ctgaagccat tcagttcatt gccgatatga tggaataaag agattaaggc
    38161 agctgacaac agctgctttt tgcgtagaag ggagtgagtt ccaatcgata attacaaacc
    38221 aactgaattt atggacaaag actcccatta tgataagtca cgtgcagatt atgccgtggc
    38281 ctttattgag ttgttgaaac acacgaaagg tcagtggttc gggaaaccgt tcaaactgat
    38341 gccttggcaa gaaaagatta tccgtgactt attcggaacg attaaggcaa atggctacag
    38401 acaattcaat acggcttatg ttgaaatacc taagaagaat ggtaagagtg agttagcggc
    38461 tgccgtggct ttgcttttga cttgcggtga tggggaagaa cgcgctgagg tatatggctg
    38521 tgctgctgat cgagcgcagg cttcaatcgt atttgacgta gctgttgata tgattcgcca
    38581 gaacccagcg ttaagcaaac gagtgaaaat cctgacttca caaaaacgaa ttatttatca
    38641 gccaacgaat agtttctatc aagtattatc agctgaagca tactctaaac acggattaaa
    38701 cagttctgcg gttgtatttg atgagcttca cgcacaacct aacagaaagc tgtttgatgt
    38761 catgacgaaa ggggcagggg atgctagaac gcaaccgcta ttctttttga ttacaacggc
    38821 agggaatgat accaattcaa tttgttatga gcagcatcaa aaagctctcg acattattga
    38881 aggtcgaaag catgataaaa cattctatcc gttaatttac ggtatcaagc aagatgatga
    38941 ttggactgat ccagaagttt ggaagaaagc caatccgtca cttgggataa cgattgcaga
    39001 agaaaaggtg gtggctgctt gtgattcggc taaacaaaat ccagctgaag aaaactcatt
    39061 cagacagctt cgcctgaacc aatgggtgaa acaagcagtg cgttggatgc caatggataa
    39121 atgggacgct tgttcattca atgcagatga agaaagtttg agaggtcgag tttgttacgg
    39181 aggtctcgac ctttccagca cgaccgacct tacatccttc gttttggtgt ttccacctga
    39241 agatgaggat gacaagtttg tgatactgcc atattactgg gtgccagaag ataccttgga
    39301 cttacgagtt aaacgcgacc atgtgaatta tgatttgtgg gccaagcagg ggtacataca
    39361 aaccactgag ggaaacgtag tccactatgg tttcattgaa aaatttattg aggaactggg
    39421 tgagaaatac aatatccggg agattgcctt tgaccgttgg ggagcagttc aaatggttca
    39481 aaacttagaa ggtatgggat ttactgtagt tcctttcggt caaggtttca aggatatgag
    39541 cccggctact aaagagttga tgaagttgac tcttgaacag aaattagccc atggcggtca
    39601 tccggttctt cgttggaata tggacaacat tttcgtccga actgacccgg caggtaatat
    39661 caaagctgat aaagaaaaat ccactgagaa gattgatggt gccatcgcga caattatggc
    39721 tctggatcgt gcaattcgtt gtggaaatga taatggtgcc agtgtttatg atgatagggg
    39781 attgttgttt ctgtgaaata tgagctttta ttacaatcca ttgatataat tgtctttgga
    39841 ggtgtatgtg tatgaatgaa tatttgattc aagtagcacc agataaaata tcttcagacc
    39901 cggacaacac attaatttct agactgtttt atagaaaatg gtggggaaaa attctaactg
    39961 aagatttgac aaagtatgct gaaggaattg ggaattatag gggagaaacg attatttctt
    40021 tcaacacaat tgctggtgca acgatacggt tattgaaatg ctataatcaa aagaactttt
    40081 caatgccaga tagtcattat gggcgtttga aagtcatttt atattccgat gatatcaccg
    40141 aagagatgaa agtgaaattt gtaaaattct atgatttaag tcatactctt gcaaatttta
    40201 tgccactata tgtatcaaaa ccttctctca atggtgttaa aggtaaaggg ccgtatcatg
    40261 actttcctga tttattttat tctgatgtaa gagataaacg atatggttca tc
//
LOCUS       DAYYAG010000009        39226 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000009 DAYYAG010000000
VERSION     DAYYAG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.009"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1144
                     /locus_tag="ACHARQ_000437"
     CDS             128..1144
                     /locus_tag="ACHARQ_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8408890.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1285..2586
                     /locus_tag="ACHARQ_000438"
     CDS             1285..2586
                     /locus_tag="ACHARQ_000438"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8408891.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2632..3294
                     /gene="deoC"
                     /locus_tag="ACHARQ_000439"
     CDS             2632..3294
                     /gene="deoC"
                     /locus_tag="ACHARQ_000439"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8408892.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3315..3713
                     /locus_tag="ACHARQ_000440"
     CDS             3315..3713
                     /locus_tag="ACHARQ_000440"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8408893.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3779..4861
                     /locus_tag="ACHARQ_000441"
     CDS             3779..4861
                     /locus_tag="ACHARQ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8408894.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5073..6635
                     /locus_tag="ACHARQ_000442"
     CDS             5073..6635
                     /locus_tag="ACHARQ_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8408895.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6628..7761
                     /locus_tag="ACHARQ_000443"
     CDS             6628..7761
                     /locus_tag="ACHARQ_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8408896.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7761..8717
                     /locus_tag="ACHARQ_000444"
     CDS             7761..8717
                     /locus_tag="ACHARQ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8408897.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8816..9982
                     /gene="deoB"
                     /locus_tag="ACHARQ_000445"
     CDS             8816..9982
                     /gene="deoB"
                     /locus_tag="ACHARQ_000445"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8408898.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10006..10824
                     /locus_tag="ACHARQ_000446"
     CDS             10006..10824
                     /locus_tag="ACHARQ_000446"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8408899.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10841..11548
                     /gene="deoD"
                     /locus_tag="ACHARQ_000447"
     CDS             10841..11548
                     /gene="deoD"
                     /locus_tag="ACHARQ_000447"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8408900.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(11846..12532)
                     /gene="gpmA"
                     /locus_tag="ACHARQ_000448"
     CDS             complement(11846..12532)
                     /gene="gpmA"
                     /locus_tag="ACHARQ_000448"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8408901.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(12704..13285)
                     /locus_tag="ACHARQ_000449"
     CDS             complement(12704..13285)
                     /locus_tag="ACHARQ_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8408902.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(13418..14101)
                     /gene="rpiA"
                     /locus_tag="ACHARQ_000450"
     CDS             complement(13418..14101)
                     /gene="rpiA"
                     /locus_tag="ACHARQ_000450"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8408903.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            14420..14833
                     /gene="rpsL"
                     /locus_tag="ACHARQ_000451"
     CDS             14420..14833
                     /gene="rpsL"
                     /locus_tag="ACHARQ_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8408904.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            14917..15387
                     /gene="rpsG"
                     /locus_tag="ACHARQ_000452"
     CDS             14917..15387
                     /gene="rpsG"
                     /locus_tag="ACHARQ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8408905.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            15481..17568
                     /gene="fusA"
                     /locus_tag="ACHARQ_000453"
     CDS             15481..17568
                     /gene="fusA"
                     /locus_tag="ACHARQ_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8408906.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            17740..18927
                     /gene="tuf"
                     /locus_tag="ACHARQ_000454"
     CDS             17740..18927
                     /gene="tuf"
                     /locus_tag="ACHARQ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8408907.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            19334..19642
                     /gene="rpsJ"
                     /locus_tag="ACHARQ_000455"
     CDS             19334..19642
                     /gene="rpsJ"
                     /locus_tag="ACHARQ_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8408908.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            19673..20302
                     /gene="rplC"
                     /locus_tag="ACHARQ_000456"
     CDS             19673..20302
                     /gene="rplC"
                     /locus_tag="ACHARQ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8408909.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            20330..20953
                     /gene="rplD"
                     /locus_tag="ACHARQ_000457"
     CDS             20330..20953
                     /gene="rplD"
                     /locus_tag="ACHARQ_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8408910.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            20953..21243
                     /gene="rplW"
                     /locus_tag="ACHARQ_000458"
     CDS             20953..21243
                     /gene="rplW"
                     /locus_tag="ACHARQ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8408911.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            21282..22115
                     /gene="rplB"
                     /locus_tag="ACHARQ_000459"
     CDS             21282..22115
                     /gene="rplB"
                     /locus_tag="ACHARQ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8408912.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            22158..22436
                     /gene="rpsS"
                     /locus_tag="ACHARQ_000460"
     CDS             22158..22436
                     /gene="rpsS"
                     /locus_tag="ACHARQ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8408913.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            22459..22806
                     /gene="rplV"
                     /locus_tag="ACHARQ_000461"
     CDS             22459..22806
                     /gene="rplV"
                     /locus_tag="ACHARQ_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8408914.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            22820..23476
                     /gene="rpsC"
                     /locus_tag="ACHARQ_000462"
     CDS             22820..23476
                     /gene="rpsC"
                     /locus_tag="ACHARQ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8408915.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            23479..23913
                     /gene="rplP"
                     /locus_tag="ACHARQ_000463"
     CDS             23479..23913
                     /gene="rplP"
                     /locus_tag="ACHARQ_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8408916.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            23903..24091
                     /gene="rpmC"
                     /locus_tag="ACHARQ_000464"
     CDS             23903..24091
                     /gene="rpmC"
                     /locus_tag="ACHARQ_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8408917.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            24116..24382
                     /gene="rpsQ"
                     /locus_tag="ACHARQ_000465"
     CDS             24116..24382
                     /gene="rpsQ"
                     /locus_tag="ACHARQ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8408918.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            24440..24808
                     /gene="rplN"
                     /locus_tag="ACHARQ_000466"
     CDS             24440..24808
                     /gene="rplN"
                     /locus_tag="ACHARQ_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8408919.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            24845..25153
                     /gene="rplX"
                     /locus_tag="ACHARQ_000467"
     CDS             24845..25153
                     /gene="rplX"
                     /locus_tag="ACHARQ_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8408920.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            25181..25720
                     /gene="rplE"
                     /locus_tag="ACHARQ_000468"
     CDS             25181..25720
                     /gene="rplE"
                     /locus_tag="ACHARQ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8408921.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            25739..25924
                     /locus_tag="ACHARQ_000469"
     CDS             25739..25924
                     /locus_tag="ACHARQ_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8408922.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            25960..26358
                     /gene="rpsH"
                     /locus_tag="ACHARQ_000470"
     CDS             25960..26358
                     /gene="rpsH"
                     /locus_tag="ACHARQ_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8408923.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            26390..26926
                     /gene="rplF"
                     /locus_tag="ACHARQ_000471"
     CDS             26390..26926
                     /gene="rplF"
                     /locus_tag="ACHARQ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8408924.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            27087..27443
                     /gene="rplR"
                     /locus_tag="ACHARQ_000472"
     CDS             27087..27443
                     /gene="rplR"
                     /locus_tag="ACHARQ_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8408925.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            27464..27964
                     /gene="rpsE"
                     /locus_tag="ACHARQ_000473"
     CDS             27464..27964
                     /gene="rpsE"
                     /locus_tag="ACHARQ_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8408926.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            27979..28158
                     /gene="rpmD"
                     /locus_tag="ACHARQ_000474"
     CDS             27979..28158
                     /gene="rpmD"
                     /locus_tag="ACHARQ_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8408927.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            28202..28642
                     /gene="rplO"
                     /locus_tag="ACHARQ_000475"
     CDS             28202..28642
                     /gene="rplO"
                     /locus_tag="ACHARQ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8408928.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            28642..29937
                     /gene="secY"
                     /locus_tag="ACHARQ_000476"
     CDS             28642..29937
                     /gene="secY"
                     /locus_tag="ACHARQ_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8408929.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            29999..30646
                     /locus_tag="ACHARQ_000477"
     CDS             29999..30646
                     /locus_tag="ACHARQ_000477"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8408930.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            30837..31055
                     /gene="infA"
                     /locus_tag="ACHARQ_000478"
     CDS             30837..31055
                     /gene="infA"
                     /locus_tag="ACHARQ_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8408931.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            31088..31204
                     /gene="rpmJ"
                     /locus_tag="ACHARQ_000479"
     CDS             31088..31204
                     /gene="rpmJ"
                     /locus_tag="ACHARQ_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8408932.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            31222..31587
                     /gene="rpsM"
                     /locus_tag="ACHARQ_000480"
     CDS             31222..31587
                     /gene="rpsM"
                     /locus_tag="ACHARQ_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8408933.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            31615..32004
                     /gene="rpsK"
                     /locus_tag="ACHARQ_000481"
     CDS             31615..32004
                     /gene="rpsK"
                     /locus_tag="ACHARQ_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8408934.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32084..33022
                     /locus_tag="ACHARQ_000482"
     CDS             32084..33022
                     /locus_tag="ACHARQ_000482"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8408935.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            33050..33430
                     /gene="rplQ"
                     /locus_tag="ACHARQ_000483"
     CDS             33050..33430
                     /gene="rplQ"
                     /locus_tag="ACHARQ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8408936.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            33702..34541
                     /locus_tag="ACHARQ_000484"
     CDS             33702..34541
                     /locus_tag="ACHARQ_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8408937.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            34517..35386
                     /locus_tag="ACHARQ_000485"
     CDS             34517..35386
                     /locus_tag="ACHARQ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8408938.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            35376..36173
                     /locus_tag="ACHARQ_000486"
     CDS             35376..36173
                     /locus_tag="ACHARQ_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8408939.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            36376..37122
                     /gene="truA"
                     /locus_tag="ACHARQ_000487"
     CDS             36376..37122
                     /gene="truA"
                     /locus_tag="ACHARQ_000487"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8408940.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            37786..37980
                     /locus_tag="ACHARQ_000488"
                     /pseudo
     CDS             37786..37980
                     /locus_tag="ACHARQ_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(38168..38944)
                     /locus_tag="ACHARQ_000489"
     CDS             complement(38168..38944)
                     /locus_tag="ACHARQ_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8408941.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            39067..>39226
                     /locus_tag="ACHARQ_000490"
     CDS             39067..>39226
                     /locus_tag="ACHARQ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8408942.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTV"
BASE COUNT        12630 a         7146 c         8553 g        10897 t
ORIGIN      
        1 tagaaggctg ccaagtcggt agataaagga ggtttttatc aaaaaacata ctttcctttt
       61 ttctctattc ttttcttggg ggttttggta cactgatagg gaatttagat agaagggagc
      121 caataggatg aagctgacca tcagagatat cgcagaaatg gcaggcgtat cagtaacaac
      181 tgtctcacag attttgaata acaaaggaag cagatttagt gataagacaa gaaaaaaagt
      241 gttgtcgatc gtgaatgaac atcattataa gcccgattat ttcgcttcaa atgtgatcaa
      301 tcgacattca aagaccatcg gaatgatcgt ccctgatgtc actgatttct tcttctccaa
      361 actgatcgaa ggtgtagaga gctatttgaa tcctttgggc tatgtgatca tgttatgcaa
      421 ttctcgtcat agccaagaaa atgagatcaa gtatttacaa gagctttccc accgctcagt
      481 ggacggaatc ttgctggcca caccaaatat tttacctgag aaatatgcat tagacagcgg
      541 tttttatcaa aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc gggataacgg
      601 acggcttatc gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa
      661 tggccatacg aagatcggca tgctgaagga aaccactgga tactatcaat tagaagaacg
      721 tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt
      781 tgaacatgga gagttgactg tccaaggtgg atatactgca tcaaaaaaat tactcaaaca
      841 taaagatatc acggctattt tttgcgggaa tgatgccatg gcgatcggat gctatcaggc
      901 gatcgatgaa ttgggcaaaa aaatcccaga agatatctca gtcataggat tcgatggatt
      961 aaagctgtca gaatacatga ttccgcaatt gacgacagtg cagcagccaa gttttgatat
     1021 tggattttat gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc
     1081 gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat tgacaaaggg
     1141 ctaactcggc ggtatattgc ttatgtaagc atttacaaag gctagttagt gcttttttta
     1201 tttttagtaa accagtttac taaagtggtt tacctttcct aaaaataatg tataataccg
     1261 aatgaagaca aggaggatca ggcaatgcga atggtggatc tgattgaaaa aaagagggat
     1321 ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac gaatggaaag
     1381 attccagatt atcaagtcag tgcattattg atggcaattt tttatcaaga catgacaaat
     1441 gaagaaatca cgaacttaac attggcgatt gctaattctg gtgatgtcat cgatttgagt
     1501 tctcttgaag gaataaaggt agataagcat tcgacaggtg gcgttggtga cacgaccaca
     1561 ttgattttag cccccctagt agctagtgtc ggagtgacag tggctaaaat gtctggacgc
     1621 ggtctaggtt acacaggtgg tacactagac aagctagaag caattcccgg atttcagatc
     1681 gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt
     1741 caatcaggaa acttggcacc ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg
     1801 accgttgatt ctcttccgct gattgccagt tcgattatga gtaaaaaaat cgcggcagga
     1861 gcagatgcaa tcgttttgga tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa
     1921 gatgcaagac gtctggcaaa gaccatgacg agcattggga aactggctaa ccgagaaaca
     1981 gtggcggtta tctcagatat gtcagaacct cttggcgaag ctatcggaaa tagtctagaa
     2041 gttgtcgagg caatcgaaac attacaagga aatggtccag aagatctagt agaaatgtgc
     2101 tacgccttag gaagccaaat ggttgttctt gctggcaaag ctaagacaac cgatgaagca
     2161 agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg
     2221 atccaaaatc aagggggaga cccagcgatc gtggaacatc cagaacgaat tttgactgca
     2281 cgctacacaa tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat
     2341 gaattaggaa ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga agaagatatt
     2401 gatcacgctg ttggattgaa actgcataaa aaaattggtg acactgtgac aaaaggtgag
     2461 tcactattga cgatctatag taacgacgaa gaaatcactt ctgtcattga attattatat
     2521 aaaaatattg aaattggtga atcggcaatg aagccaacgt tgattcatga catcatcaca
     2581 gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta
     2641 aaccgaatga tcgaccatac tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa
     2701 attatcgaag aagcaaaaaa ataccatttt tattctgtct gcatcaatcc agcatgggtt
     2761 gcgctagcga aagaacaatt acaaggtgag ccagtagccg tatgtacagt aatcgggttt
     2821 cctctaggag caaacacacc cgagacaaaa gcatttgaaa caacagatgc catcaataac
     2881 ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa
     2941 caagtccaaa aagatatcga agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa
     3001 gttattattg aaacatcgtt attagataga gaagaaatca tccgagcttg cgaaatagca
     3061 aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag
     3121 gtagaggatg tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc
     3181 ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg aagctggtgc tacaagaatc
     3241 ggaacaagcg caggagtagc aatcgtatct ggttcaactg gtgaaggcta ttaatttttt
     3301 caaaggagaa gctcatggca gaactcaaac aagaatggat caacacagct attgaagcgt
     3361 tagataaagc ctatgttcct tattcccatt ttccggtagg cgcttgtctc gtaacggaat
     3421 caggcaagat ttatcaaggc atcaacattg agaatgcatc atttggatta acgaattgtg
     3481 ccgagcgcac agcttttttc aaagcagtat ctgaaggaga aaaaacattt acgcatctag
     3541 tcgtagcggg gcacaccccc gatcctatct ctccttgtgg tgcatgtcga caagtaatgg
     3601 cagaattctg ttcccctgat atgccggtta cgctagtagg agacaatggt gtgacgaagg
     3661 caacgaccgt tcgtgcactg ctgccttatg catttactga aaaagatcta taactcatgt
     3721 tcattcagtc agctgactgt tgaaaaataa aacaattatt ttaggaggct ttacagaaat
     3781 gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta gcagcagggt tatttttagg
     3841 tgcatgcggc aacaacggct ctactgattc atccggtggc aaaagctcat cagacacaac
     3901 aactgcagca ttgatcacag acacaggtgg tgtagacgac cgttcattca accagtcagc
     3961 ttgggaagga ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa atgatggctt
     4021 ccaatatttc caatcttcaa atgaatcaga ttacatccca aatattgacc aagcattgaa
     4081 tgctggattc aaaacgattt ttggtattgg ttataaacta aaaccagcga tccaagaaca
     4141 agcaacaaac aatacaggta cgaactttgt tattatcgat gatgtcatcg atggattaga
     4201 taacgttgta tctgcaactt tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc
     4261 agcttatacg actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat
     4321 cgatcgtttt gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa
     4381 agaaatcaaa gtattgaatc aatatgctgg tgacttctct gctccagata aaggacgttc
     4441 aattgctcaa ggtatgtatg cacaaaacgc agatatcatc ttccatgctt caggcggtac
     4501 aggtaacggt gtattccaag aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg
     4561 ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa tacacattaa acggcgaaaa
     4621 gaaaaacttc acattgacat ctacactgaa agcagttgga acagtagtag aagatttggc
     4681 acaaaaatca gcggacggta aattccctgg cggcgaacac actgtttatg gattgaaaga
     4741 agacggtgtt ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag cagttgatga
     4801 agcaaaagaa aaaatcattt caggtgacgt aaaagttcca gaaactcctg aagaaaacta
     4861 aaaccatttc tatagccttg ttagacacat gtttgacaag gctatggttt gattagaaca
     4921 agaattgttc atttatccaa gctgccttgt gacagtttgg ataagggaac aactccctga
     4981 agtaatgttt gtttcaaaag aagaacgctc actaagcgtg aatcttagtc aatcgttgcc
     5041 ctagatagaa atcgttagga tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa
     5101 tgcagaatat taccaaagaa tttggaacat tcaaggcaaa tgacaacatc aatttacaag
     5161 taaaggcagg agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa tctacattga
     5221 tgaacgtgct ttccggatta ttagagccga catcagggaa aattttgatg cgtgggaaag
     5281 aagtaaagat cacaagtccg accaaagcca accaattagg gattgggatg gtccatcagc
     5341 actttatgct tgttgatgcc tttactgtaa cagaaaacat cgtgttggga agcgagccta
     5401 gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac
     5461 agtatggatt gtcagtcaac ccagatgctt acgttcgtga tatttcagtt ggaatggaac
     5521 aacgggtaga aattttaaaa acactttacc gaggagcaga tgtactgatt tttgatgagc
     5581 cgacagctgt attgacccct caagaaattg atgaattaat cgtgatcatg aaggaattag
     5641 tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc aaagcagtag
     5701 ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg
     5761 taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc aaaacgatga
     5821 aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa
     5881 atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc gaagtacttg
     5941 gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg actggtttgc
     6001 gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat aaaaaacccc
     6061 gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa tacgggttag
     6121 tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa aaaccttaca
     6181 gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag
     6241 aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca ggcggaaatc
     6301 agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg attgttgcaa
     6361 atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac
     6421 aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa attttaaatg
     6481 tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt gatccgaaag
     6541 aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa gaagcacgaa
     6601 aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta
     6661 ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt gatggctgct
     6721 tttggttttc agcccatcca aggttattct tctatgctag atgcagccct tggttcgcaa
     6781 agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc attaggtttt
     6841 tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc actttgcggg
     6901 tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt tgttttgctt
     6961 ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt tccaggttta
     7021 cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta
     7081 ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg
     7141 gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt aacacaagct
     7201 tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct tattttcgtt
     7261 tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg attgaatcct
     7321 tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc gatggtcatt
     7381 tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta tcaaaacttc
     7441 tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc cttgcttggt
     7501 tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt gaagatcggt
     7561 ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct
     7621 ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc
     7681 caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac
     7741 catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc cttacttcta
     7801 cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt tctgaacgaa
     7861 gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt agttccatcg
     7921 ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc gctattttag
     7981 tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca gtcagtttgc
     8041 gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct ttaggtgttt
     8101 tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag aatctggggt
     8161 atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc tttaaaaata
     8221 cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg atcatcttca
     8281 agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct gccgacacat
     8341 taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc ttgttaggcg
     8401 gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct ggtacgatcg
     8461 ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat cctttagggg
     8521 caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt ggcgcacaat
     8581 taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat ttgctgacaa
     8641 tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac gggaagaact
     8701 atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga agtgtttttc
     8761 gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa taatcatgtt
     8821 taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac cagatgccga
     8881 taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag caggattgaa
     8941 cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag gtgtggaatc
     9001 agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag ggaaagatac
     9061 gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc gtgtgtttcc
     9121 taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt
     9181 atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac accaaatgaa
     9241 aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg cggcacatga
     9301 agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg atataacaaa
     9361 agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga
     9421 cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac acacagtcct
     9481 tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca acgatatttt
     9541 caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg gcgttgacca
     9601 gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt tagtagattt
     9661 tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt
     9721 tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc taatgattac
     9781 agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag aatatgtacc
     9841 gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag gatattattc
     9901 agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa atggcgaaag
     9961 cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac caagttaagc
    10021 gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga aattgatttt
    10081 ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg
    10141 atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca tgcaggaaag
    10201 ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag attccactac
    10261 tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc cggactaggg
    10321 gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt tgtaccagga
    10381 gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt gatcggtccg
    10441 aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac gcctgactat
    10501 caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat
    10561 atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc tcggacaata
    10621 ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga
    10681 ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca agcgaatctg
    10741 agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa agcattggtt
    10801 aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc atattgaagc
    10861 gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc gagctaaata
    10921 tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg gtatgttagg
    10981 ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc
    11041 atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga aattgattcg
    11101 agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca
    11161 aggtgcagcg accagttctt cgatgatcga aaaaaatttc caagcctttc attttcctcc
    11221 aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa agggctatac
    11281 ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt tgacagagac
    11341 gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg ctctttatta
    11401 cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg accatttgat
    11461 caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt
    11521 aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc tgcattaagt
    11581 aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg acttattatt
    11641 gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaatctgccg tttaactctg
    11701 agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt gtgacttgtg
    11761 accgagaagt tggcaggaga aaaccgttta tacgaaaaaa gggactgtga caaatcgtgt
    11821 cacagtccct tttttatagt gttttttata ggtagtattt tttagcaact gttaagtcat
    11881 catttaattc ataaacaagt ggttgacctg ttggaatttc aagatccatg atgtcttcgt
    11941 cagagatacc ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt gcagctacta
    12001 aaacagtttt gttgtctagt aatgctggag caatttcatc ttgccagaat ggcaaagcgc
    12061 gttctaatgt cactttcaag ttttctccac caggaatatc gcgtttgtct aacattgcgt
    12121 aacgacggtc atttgctgca gaaccttcgt cagtttcttc cattaatgga ggaagagtat
    12181 cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta tttttcagct gtttcttttt
    12241 tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa gcgccatgat ttgatttgtg
    12301 gtacccaaag ttgatctgaa tattctaaga tcaagttaca tgttttgatt gcacgagtta
    12361 aaacagaagt gtaagcaaca tcaaattcga ttccagcttc tttgattttg cgtccgcctt
    12421 cttttgcttc ttcgattcct tctggtgcta gatttacatc agcccaacca gtaaattggt
    12481 tcaatgcatt ccattcacta agaccgtgac gagaaaaaac taattttggc atgtttagct
    12541 ctccttttaa gataggttta tatttctgaa attttcagtt ctgttttatt gtacaatttt
    12601 ttggaagaga tttccatcga aaaattagat aagattctat gagaaaacaa gtagagaata
    12661 taaaaaaacg aacagtgccc acgcaactgt tcgtttttag gtattagatg cataattgct
    12721 cgatttcttt ttcaggtaaa ttgatatatt tataacatgt tccaactgaa acaccgcatt
    12781 ttgaagaaat aaattggatc gtttcttttt tttcataata gagacgtcgg atttttttga
    12841 tcgtttttgc atcgattttt ggtcttccgc cgatttttcc ttttttacga gcttcatcta
    12901 atccaactaa tgtacgttct ttgatcaatg cgcactccat gtttgctagg ttttccatta
    12961 attggaaata gatttttccc atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt
    13021 gcagcccgcg atctttgaat aattgcgtga gttctgttaa ttgccgcgta gatttgccta
    13081 atcgatgaag ttggcaaatg actagtgcat cgccttgttg catgtcagcg atcactgact
    13141 ccaaatgaac agcagtagaa tcattttgtt ccacttcata tgtctcatgg aaaatttctt
    13201 gacaaccaaa gtcagataat tgatcaacct gagttgttaa atcaacgtca tttatggttg
    13261 tgcgtgcata accaataatt ttcatggtga ttcctttcta gcagtgattt tctaactgga
    13321 tataacagta aaaatgtagc ataaagtacg tcaaaaagat agttttttcg caaattttcc
    13381 gctgtctttt tttattggat gatcaagcag tttttcttta tttacgacgg attgcgtcaa
    13441 gaatctcaac gcctttttct gagccgacaa ctactttaga aaccatgttt aggaaaagcc
    13501 catgttctac taccccaaca agttcatcta aataggtagc taagctttct ggctgttcga
    13561 tgacttctag atgcagatca atgatgtaat gaccaccatc tgtggttaag acttctccgt
    13621 tttcgtctgt acgcaagacg ggttggaatc ttttttcttc aaaaatatga agtagctgtt
    13681 gggagccata agggatcact tcaactggga gtgggaactt cccaagttta tcgactaatt
    13741 tagaagaatc aacgatccaa atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa
    13801 gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc actgatttca tcagcaccgt
    13861 ctattgtcaa atcgactacg gggacttcat caatactctt taacggtatt cctaattttt
    13921 ccgcttgttc ttttgtcgca tttgaagtag taactcctgt gatacgtaaa ccttcttcac
    13981 gcatacggcg accaatctct tctaccatat aataggcggt tgaaccggtc ccgagaccta
    14041 cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc agctaattgt tttaaattca
    14101 tctttgcgtc cacctttttt tagtttgagc atcgtgcttt cctcactatt ttagcgtgat
    14161 ttacggtaaa aagataccat tcttttttat tccaataaag ctttttcaaa aaaaattgca
    14221 tctcttgttg acatgttttt tcattcgtga tattctaaca aaggtgcttt atctacaggt
    14281 ttctgtgaag aaacgtgctg actgtttatc aaagcatatt tgataaggat gcagacattg
    14341 catttatttt ttatcacaaa aaaagaacca cctggatgtg tggaactaga cgcaaatcgt
    14401 gggaaggagg aaacaaggaa tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc
    14461 aaaggtggaa aaatctaatt cacctgcttt gaacaaagga tataacagct ttaagaaaac
    14521 tcaaactaac gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac
    14581 acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt caaacttaat
    14641 tgaagttact gcttatatcc cagggattgg tcataactta caagaacaca gtgttgtatt
    14701 attacgcggt ggtcgtgtaa aagacttacc aggggtacgt taccatatcg ttcgcggtgc
    14761 tttagatact gctggtgtta acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa
    14821 acctaaagct taattttaat tattaaacaa atgaaacttg cttctactat atagaagtaa
    14881 ttactatgaa caatatttcg gaaggaggag ttacggatgc cacgtaaagg tcctgttgca
    14941 aaacgtgatg ttttaccaga tcctatttat aactcaaaat tagtaactcg cttgatcaac
    15001 cgtgtaatgg ttgacggaaa acgcggtatt gcagctaaca ttatctataa tgcctttgac
    15061 ttgatcaaag aatctacagg taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac
    15121 gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta ccaagtacca
    15181 gttgaagttc gccctgaacg ccgcgttact ctaggtcttc gttgggtagt aaactacgct
    15241 cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa aagaaatcat ggatgctgca
    15301 aacaacacag gtgcttctgt taaaaaacgt gaagacactc ataaaatggc tgatgccaac
    15361 cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt tggtcggctt ttataagtcg
    15421 acctcagata gtgtccttat ttttagcaac tagatagtaa agagaggaga aacaaggaga
    15481 atgaccagag aattttcttt acaaaacact cgtaatatcg gtatcatggc ccacatcgat
    15541 gctggtaaaa cgactgctac tgaacgtatc ttgtactata ctggtaaaat ccataaaatt
    15601 ggtgaaactc atgaaggagc ttcacaaatg gactggatgg aacaagaaca agaacgtggt
    15661 attacgatca cttctgctgc tactacagca caatggaagg gtcaccgaat caacatcatc
    15721 gacacaccag gacacgtaga cttcacagtt gaagttgaac gttcattgcg tgtacttgat
    15781 ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg
    15841 cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg caaacaaaat ggacaagatc
    15901 ggtgctgact tcttatattc agtatcaact ttacatgacc gtttgcaagc aaacgctcac
    15961 ccaatccaat taccaattgg tgcggaagac gacttcacag gaattatcga ccttgtaaca
    16021 atgaaggctg aaatgtatac aaacgatctt ggaacagaga tcgaagaaac agaaattcct
    16081 gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa
    16141 actgatgaag aacttacttt gaaatactta gaaggtgaag aaatcactga agctgagtta
    16201 aaagaaggta tccgccgcgc aacagttaac gtagaattct acccagtact atgtggttca
    16261 gcatttaaga acaaaggggt tcaattacta ttggacgctg ttcttgatta cttgccatca
    16321 ccgttagata tcccagctat caaagggatt gaccctaaaa ctgatgaaga agtagaacgt
    16381 cctgcagacg acagcgctcc tttctcagct ttagcattta aagttatgac tgacccattc
    16441 gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta
    16501 caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta tcctacagat gcatgccaac
    16561 tctcgttcag aaatctctga agtttacgct ggagatatcg ctgctgctgt tggtttgaaa
    16621 gacacaacta ctggggacac actatgtgat gaaaagagtc tagttattct tgaatccatg
    16681 gaattcccag aaccagttat ccaagtcgct attgaaccta aatcaaaagc tgaccaagat
    16741 aaaatgggtg tggctttaca aaaactatct gaagaagatc ctactttccg tgctgaaaca
    16801 aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg aacttcactt agatatcatc
    16861 gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc tcaagtatct
    16921 taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta agtttgtacg tcagtctggt
    16981 ggtaaaggtc aatacggtca cgtatggatt gaatttacac caaatgaaga aggtgctggt
    17041 ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat tccagctgtt
    17101 gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc attagtagat
    17161 atcaaagcta aactttacga tggttcatac catgatgtcg attcaaatga aactgccttc
    17221 cgtgttgctg cttctatggc attacgtgca gcagctaaga aagctaatcc agtaatcctt
    17281 gaaccaatca tggctgtaga agttgttatc cctgaagatt atttaggtga tgttatgggt
    17341 cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa
    17401 gttgttcgtg cgatggttcc acttgctgaa atgtttggtt atgctacaac tcttcgttct
    17461 gctactcaag gtcgtggtac atttacaatg acatttgacc actacgaaga tgtaccaaaa
    17521 tcagtacaag aagaaatcat taagaaaaac ggcggaaaag ctgaataatt tcttgaaacc
    17581 ggatagtcct gcaaagagat tatccaaaga gactatattt ttaatatagt ttcgtgtaaa
    17641 ataattatcg atgatatggg catgtggtgt accttgtgta catcatatac ctatcagaca
    17701 aataaaaaat atattgtatt tttaggagga cattttaaaa tggcaaaaga aaaatttgac
    17761 cgttctaaac cacacgttaa cattggtact atcggacacg ttgaccatgg taaaactaca
    17821 ttaacagctg caatcacaac tgtactatct aagaaaaacg gcggccaagc tatggcttac
    17881 gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa tcacaatctc aacagctcac
    17941 gttgagtatg aaacagatac acgccactac gctcacgttg actgcccagg acacgcggat
    18001 tacgttaaaa acatgatcac tggtgctgct caaatggacg gagctatctt ggtagtttct
    18061 gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg tcaagttggt
    18121 gttccttaca tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga agaattacta
    18181 gaattagttg aaatggaagt tcgtgaccta ttaacagaat acgaattccc tggtgacgat
    18241 gttcctgtag ttgctggatc agctttgaaa gctctagaag gcgacgcttc atacgaagaa
    18301 aaaattcttg aattaatggc tgcagttgac gaatacatcc caactccaga acgtgacaac
    18361 gacaaaccat tcatgatgcc agttgaagac gtgttctcaa ttactggacg tggtactgtt
    18421 gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg acgaagttga agttgttggt
    18481 attgctgaag aaacttcaaa aacaacagtt actggtgttg aaatgttccg taaattgtta
    18541 gactacgctg aagctggaga caacattggt gctttactac gtggtgttgc acgtgaagac
    18601 atccaacgtg gacaagtttt agctaaacca ggtacaatca cacctcatac aaaattctct
    18661 gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcactaac
    18721 taccgtccac aattctactt ccgtacaact gacgtaacag gtgttgttga attaccagaa
    18781 ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg aagttgaatt aatccaccca
    18841 atcgctatcg aaaacggtac taaattctca atccgtgaag gcggacgtac agttggtgcc
    18901 ggtgtcgtta cagaaatcaa agcttaattc acttaagttt taaagtaaag caacatttat
    18961 tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg ttccgagctt ttttatttat
    19021 tgcaggatca ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa ataaaagagt
    19081 cagtattttc taaatgaaac tttatgaaaa caaaatgttt tttacaagaa aaagccttgc
    19141 tttgtgagtt tatatatatt ataatgacaa aggtgcgttt actttagtga attgcacaga
    19201 cgtaatcaga atgatttcgt cggctaagaa acgagaggtt gcgacacgcc cggacgcttt
    19261 gccacgaacg agcgtgacgg aaattttcgt ggagctatgt ctacttttca aaataggcga
    19321 aggagggaac aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg
    19381 tattttagat caatcagcgg ataaaatcgt ggaaactgca aaaagaactg gagctagcgt
    19441 gtctggtcca attccattac caactgaacg cagtctttac acaatcatcc gtgcgactca
    19501 taaatacaaa gattcacgcg aacaattcga aatgcgtaca cacaaacgtc ttatcgacat
    19561 tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag ctagacttac catctggtgt
    19621 aaacatcgaa atcaaattat aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa
    19681 aggaatctta gggaaaaaag tgggaatgac acaaatcttc actgaatctg gtgaattaat
    19741 tccagtaact gttgtagaag ctactccaaa cgtagtacta caagtgaaaa caatggaaac
    19801 ggatggctac gaagctattc aagttggtta ccaagatatg cgtgaagttt tatcaaacaa
    19861 acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt
    19921 caaaaatgtt gagctaggag aatatgaagt aggaaaagaa attacagtag atgttttcca
    19981 agcaggagac attattgatg taacaggtac tacgaaaggt aaaggattcc aaggggttat
    20041 caaacgccac ggacaaagcc gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc
    20101 tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg
    20161 tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag
    20221 aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa ttactatcaa
    20281 atcagctgtg aaagctaaat aattgtagga agaaaggagg aactaaggaa tgccgaatgt
    20341 agcattattc aaacaagatg gaagccaaaa cggtgaaatc acacttaacg aagaaatctt
    20401 cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt
    20461 aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg gacgtaaacc
    20521 atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct atccgctcac cacaatggcg
    20581 tggaggtggc gtagtctttg gaccaacacc acgttcttac agctacaaac ttcctaaaaa
    20641 agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt
    20701 agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc
    20761 taacttatct atcgactcta aagtattggt tgttctagaa aaaggtaacg actttgctgc
    20821 tttatcagca cgtaacttac caaacgtttc tgtagtaact tctgataatg tgagtgtgct
    20881 agacgttgta tcaaatacaa aagtgttagc aacacaaact gctcttactc aaattgagga
    20941 ggtgcttgca taatgaactt actagacgta atcaaacgcc cagtgatcac tgaaaaatca
    21001 atgcttgcca tggatgacaa gaaatacact tttgaagtgg acactcgcgc aaacaaaact
    21061 ttagtaaaac aagcggtaga agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg
    21121 aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc
    21181 aaagcgatcg ttacattgac tgaagattca aaagaaatcc aattattcga agctgctgaa
    21241 taagcactaa tagataactc attaaattag gagggaaaaa cgtggcgatt aaaaagtaca
    21301 aacctacctc aaatggtcgt cgtaacatga caggttctga ttttgctgaa atcacaacat
    21361 caacacctga aaaatcattg ttacaaccat tgaaaaacca tgctggacgt aacaacaacg
    21421 gccgcatcac tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc gtgatcgact
    21481 tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc
    21541 gttcagctaa catcgcgtta gtacactatg aagatggagt gaaagcttac atcttagcac
    21601 ctaaaggact agaagtgggt atgaaacttg tttcaggtcc agaagcagat atcaaagtcg
    21661 gaaatgctct tccattagaa aacattccag ttggtactgt tatccacaac attgaaatga
    21721 aacctggtaa aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa gtacttggta
    21781 aagaaggcaa atacgtattg atccgcttga actctggtga agttcgtatg atcttagctg
    21841 catgccgtgc tacaatcggt tctgtaggta acgaacaaca cgaattaatc aacatcggta
    21901 aagctggacg ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc
    21961 ctaatgatca cccacacggt ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag
    22021 tttctccttg gggtcaacca gctcttggtt acaaaactcg taacaaaaaa gctaaatcag
    22081 acaaacttat tgttcgtcgt cgcaaaaata aataattact ttgaccatgt gtcgcacatt
    22141 ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg accttttgtc gatgagcatt
    22201 taatgaaaaa agttgaagct caagcagggg ttgaaaagaa aaaagttatc aaaacttggt
    22261 cacgccgttc aacagttttc ccaagttttg ttggatatac catcgcagtt tatgatggac
    22321 gtaaacatgt accagtttac atccaagaag acatggtagg acacaaatta ggtgaatttg
    22381 cacctacaag aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg
    22441 aggggaggat aaatccaaat ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca
    22501 gttcgcactt cacctcgaaa agcccgttta gtaattgacc ttatcagagg taaaagtgtc
    22561 gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa
    22621 gtattgatgt cagcagttgc gaatgcagaa aataactttg acttagatgt tgaaaacttg
    22681 gtagtaagcg aagcttttgt taacgaagga cctacaatga aacgtttccg tcctcgtgca
    22741 aaaggatcag cttctccaat caacaaacgt acaagtcata tcacggtagt cgtatcagaa
    22801 aaataaggag ggataatcag tgggtcaaaa agtacatcca attggaatgc gtgtaggcat
    22861 catccgcgac tgggatgcta aatggtatgc tgaaaaagag tatgctgagt tcttacacga
    22921 agatttaaga atccgtaaat ttatttcaac taaacttgct gatgctgctg tatcaacagt
    22981 tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta aaccaggtat
    23041 ggttattggt aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa acaaattaac
    23101 tggtaaaaga atccatatca atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt
    23161 agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa
    23221 acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg
    23281 tcgtctaaac ggagcagata tcgctcgttc agaaggatac tcagaaggaa ctgttcctct
    23341 tcatacttta cgtgcggaca ttgattacgc atgggaagaa gcagatacaa catacggaaa
    23401 actaggagtt aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa aaaacactga
    23461 gaaaggaggg aaataatcat gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc
    23521 ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag cattcggtga atacggtttg
    23581 caagctgttg attcacattg gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg
    23641 actcgttaca tgaaacgtgg tgggaaagta tggattaaaa ttttccctca caaatcttat
    23701 actgccaaag caattggggt acgtatgggt tctggtaaag gggcacctga aggatgggtt
    23761 gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga agaagtagct
    23821 cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt
    23881 gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc agagaattaa ccactgccga
    23941 aatgcttgat caagaaaaac aattaaaaga agaattgttt aatcttagat tccaattagc
    24001 aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat
    24061 caaaactgtg ttgcgtgaac aagctaagta attatggaag gaggccatta agcgtatgac
    24121 tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg gtttcagaca aaatggataa
    24181 aacaattacc gttgctgtcg aaacaaagaa aaaccaccct atctatggca aacgcatgaa
    24241 ctattctaag aaatacaaag tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt
    24301 gagaattatg gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt tggaagtagt
    24361 cgaagaagca gttattatct aataacacga gattttccta tatagattga atgcaaaatc
    24421 tgaaaggagg atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc
    24481 aggcgcacgt gaaattttga cgatcaaagt ccttggtgga tctggtcgta aaactgctaa
    24541 catcggtgat gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa
    24601 aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg
    24661 ttcatatatt aaatttgatg aaaatgctgc agtgattatt cgtgatgata aaagccctcg
    24721 tggaacacgt atctttgggc cagttgcacg tgaactacgt gaaaacaact tcatgaagat
    24781 cgtttcacta gcaccagaag ttttataatc ttgagacact tatcaaagga ggtgcgaaac
    24841 agttatgttt gttaaaaaag gcgataaagt taaagttatc actggaaaag acaaaaacaa
    24901 agaaggcgtt gtactagcag cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa
    24961 tgtcgttaaa aaacaccaaa aaccttctca agctgctcct caaggtggaa ttgttgagat
    25021 ggaagcgcca attcatgttt ctaacgtgat ggtggttgac tctactggtg tagctggccg
    25081 agttggctac aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt
    25141 tttagataaa taaaagttta aggaaggagg gtcttactga atgaaccgcc taaaagaaaa
    25201 atatcttaaa gaagtaactc catcattgat ggaaaaattt aactatagct ctgttatgca
    25261 aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc
    25321 taaaaactta gataaagcag ttgaagaatt aactttgatc actggtcaaa aaccaatgat
    25381 tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc caattggtgc
    25441 aaaagttaca ttgcgcggag aaagaatgta cgaattctta gacaaattgg tttctgtttc
    25501 actaccacgt gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa
    25561 ctatacttta ggtattaaag aacaattgat cttcccagaa gtagattatg atttagtaga
    25621 taaagtacgt ggtatggaca tcgttattgt aacaacagca aactctgatg aagaatctcg
    25681 tgagttgttg acacaattag gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt
    25741 ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa cacaagctta
    25801 tactcgttgc gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc atctttgccg
    25861 tatttgcttc cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga aagctagctg
    25921 gtaaaaagta aattcgcata agaaggaggt aaaagatcaa tggtcatgac agatccaatt
    25981 gcagattttc taactcgtat ccgtaacgca aacatggtta aacatgagtc tttagaagta
    26041 cctgcatcaa aaattaaacg tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt
    26101 gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt
    26161 aaaaacggcg aacgtgttat cacaaactta aaacgtatct ctaaaccagg tttgcgtgct
    26221 tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt taggtatcgc aatcatttcg
    26281 acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt
    26341 atcgcttacg tatggtaatc aagtagaatc tacaaggagg tgtctttaag tgagccgtat
    26401 cggaaataaa gtcgttgtta tccctgaagg agtaacagtt actcaagacg gaaacaacgt
    26461 tacagttaaa ggaccaaaag gtgaattgac tcgtactttt tcagcagata tcaaaatgaa
    26521 tatcgaagga aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat
    26581 tcatggaaca actcgtgcga acttcaacaa catggttgta ggtgtcagcg aaggtttcca
    26641 aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa ttacaaggat caaaacttgt
    26701 attgaacgtt ggttactcac atccagtaga aatcacacca ccagctggag taactgttga
    26761 agtaccagct aacacacaag taattgttaa aggtgcgaac aaagaagaag ttggcgaatt
    26821 agctgcaaac attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta
    26881 tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt aaataatcgt ttagaaaaat
    26941 tgcatagaag ccagcagcag gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac
    27001 atttttactt gtgccgcagc ttctattttt cgaaacgtat ttactgcttg aaatagtagt
    27061 acaataaaaa taaagaggtg acaattgtga ttacaaaacc agataagaac aaaacacgtc
    27121 aaaagagaca tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc ccacgcttga
    27181 acgtttttcg ttctaacaaa aacatctacg ctcaaattat tgatgacgta gcgggtgtaa
    27241 cgctagcaag tgcctctacc ttggataaag aaatttcagg tggaacaaaa acagaagcag
    27301 cacaagctgt cggtaaacta gttgctgaac gtgcagctga aaaaggcatt aaagtagtag
    27361 tctttgaccg tggtggatac ctttaccatg gccgtgtgca agctttagct gaagctgctc
    27421 gcgaaaatgg actagaattt tagaagaagg aggaatacca ttcatggttt atatcgatcc
    27481 aaaacatttg gaattagaag accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa
    27541 aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt
    27601 agggtttggt acaggtaaag ctcaagaagt tcctgaagca atccgtaaag caattgaaga
    27661 tgcgaagaaa aacttagttg aagtacctat ggttggttca acaatcccac acgaagtaat
    27721 cggtgctttt agtggtggta gaatcctaat gaaacctgct gtagaaggtt ctggggttgc
    27781 tgctggtgga ccagttcgtg ccgtattaga attagctggg gtagcagata tcacttctaa
    27841 atctttaggc tcaaatacac caatcaacgt tgttcgcgca acagttgaag gtttgaaaca
    27901 attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg
    27961 ataaggagga caacaataat ggctgaatta aaagttactt taaaacgcag tattatcgga
    28021 cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc ttacaaagat taacagttca
    28081 gtggttaagc cttctaatga agcaattaaa ggcatgatca atactgtttc tcacctagtg
    28141 gacgttgaag aaatttaatt tagaaaaatc ataactagaa cattaaggag gtgccaaacc
    28201 tatgaaactt catgaattaa aacctgctga aggttcacgc caagtacgta accgtgttgg
    28261 tcgtggtact tcatctggta acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc
    28321 tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga caaacaccat tgttccgtcg
    28381 tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga
    28441 tgtattgaac cgctttgaag acggagctga agtaactcct gttgctttag ttgaagctgg
    28501 aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct aatggggaac taaacaagaa
    28561 attaactgtg aaagcagcaa aattctctaa agcagctcaa gaagcaattg aagcggctgg
    28621 tggatcaatc gaggtgatct aatgtttaga ctattaaaag atgcttttaa agtaaaagac
    28681 attagatcga aaatcctatt tactgtattt gtgttgtttg tatttcgcct aggtgcacat
    28741 attactgtac caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt gccatttttc
    28801 aacatgttga acttggtaag tggtagtgcg atgcaaaact tttccatttt ctcaatgggg
    28861 gtttcaccat acattactgc ttctatcgtc attcagcttc ttcaaatgga catcgtgcct
    28921 cgatttgttg aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa tcaagcgact
    28981 cgatatctga cattagtatt ggcatttatc caatctgtcg gtatcactgc tggattcaat
    29041 caatacgctc aattaggttt cgtgaataat cctaacgtag caacttacgt aacgatcggt
    29101 ttgattctaa cagccggtac gatgtttgtt acttggctag gagaacagat cacagaaaaa
    29161 ggtatcggaa atggcgtatc aatgatcatt tttgcaggta tcatttctag attgcctgaa
    29221 agtatcagag aaatttatga agattacttt ataaatattg atccttcaga catatggaaa
    29281 tctgctattt ttgtggccat tttactagta gcggtcttgt tgatcgtgac attcgtaaca
    29341 tttttccaac aagctgaacg caagatcccg atccaatata cgaaacgcgt agctggcgcg
    29401 cctacaagca gctatctgcc gttgaaagta aatgcagctg gagttattcc agttatcttt
    29461 gctagttcgt ttattgctac gccaaatgct gttttacaag cattgagcgg tagattcggt
    29521 tctgaaaact ggtacaaagt gatgatggag atctttaact ataatacagc acctggtgct
    29581 acgatttata cgattctgat cgttgccttc actttcttct acgcctttgt tcaagtaaac
    29641 cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt atataccaag cgtacgccct
    29701 ggtaagggga ctgaagagta tgtctcaaga ttactgatgc gtttaagtac agttggtgca
    29761 gtcttccttg gtttagtggc attgcttcca atcattgctc aaatggtttg ggatcttcca
    29821 caatcaatcg gattaggcgg tacaagctta ctgatcgtta tcggtgtagc tttagaaact
    29881 gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg ttggattcat tgagtaagaa
    29941 agcgtgttga ggtttcccct caacacacgt tctcacacat ttattaggag ggaaaacaat
    30001 gaacctcatt ttaatggggc tgccaggcgc aggtaaagga acacaagctg aaaaaatcat
    30061 tgatgcttat ggtattccgc acatctcaac aggagatatg ttccgcgcag cgatgcaaaa
    30121 cgaaacagct cttggcctgg aagcaaagtc ttatatggat aaaggtgcat tagttcctga
    30181 tgaagtaaca aatggaatcg tgaaagagcg actagctgaa ccagatacag agaaaggctt
    30241 cttattagat ggctttccac gtacgctaga tcaagcaaaa gctctggacg caatgctaaa
    30301 agatttgaat aaaaaaattg atgctgtcat cgatatccat gttggtgaag aaatcttggt
    30361 cgaacgtcta gccggccgat ttatctgcag caattgtgga gcaacttacc ataaaatttt
    30421 caaccctaca aaagttgaag acacatgtga tcgttgcggt gggcatgaat tctatcaaag
    30481 agaagatgat aaacctgaga cggttaaaaa tcgtctggct atcaacatca aaaatagtga
    30541 accaatcttg gcttattata aagaacaagg tttgctaaac actattgatg gtgctcgtga
    30601 aatcgatgct gtatttgcag atgtacaaaa aatcattgaa aaatagcacg attgcccgta
    30661 gccgacttgc attatgcagt tactcttgtc aagttcaata aattatgata gaatatgtca
    30721 gtccgacttc tagagaatcc tctagatgat ccattttgag aattttgagg tgggaactgc
    30781 agtttccgcc attttatcgt gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg
    30841 caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt
    30901 ttaaagttga actagaaaac ggacaccaag ttcttgctac tgtttcaggt aaaattcgta
    30961 tgcattacat ccgtatctta cctggtgaca aagtaacggt tgaattgtct ccatacgatc
    31021 ttaaccgtgg ccgtattact tatcgcttta aataattgta ctccgtaaaa ctacaggagg
    31081 tattatcatg aaagtaagac catcagtaaa accaatgtgc gaacattgta aagtaattcg
    31141 tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca aaacataaac aacgtcaagg
    31201 ataattggag gtgctataaa tatggctcgt attgcaggag tagatattcc tcgtgataaa
    31261 cgtgtagtag tttcacttac ttatatctat ggtattggta acactactgc taaaaagatc
    31321 ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca
    31381 gatgctatcc gtgcagaagt agataaatta aaagttgaag gggatcttcg tcgtgaagtg
    31441 aacttgaaca tcaaacgttt gatggaaatc ggttcttacc gtggaatccg tcatcgccgt
    31501 ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa aggcccagct
    31561 cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa cttcatggca
    31621 gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg tattgcacat
    31681 atccattcta cattcaataa cactatcatc atgatcactg atactcacgg aaatgcatta
    31741 gcgtggtcat cagctggttc attaggtttc aaaggaagca aaaaatcaac tccttttgcc
    31801 gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg aacacggatt aaagactgtt
    31861 gaagtaacag ttaaaggacc tggttctgga cgtgaagcag caattcgttc attacaagct
    31921 acaggtttag aagtgactgc aattcgtgac gtgactccag ttcctcataa tggtgcccgc
    31981 cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat cagtaacgtc
    32041 attgaacaag acacttttcg ttttgaaagg ggtaaagata agaatgattg agttcgaaaa
    32101 accaagaatc gcaaaaattg atgaagaaaa agattatggc aagttcatcg ttgaacctct
    32161 tgaaagaggg tatgggacta ctttagggaa ttccctacgt cgtattttat tatcttcttt
    32221 gccaggtgct gccatcacta atattcaaat cgatggtgta ctgcatgaat tctctaccat
    32281 caaaggtgtt agagaagacg tcactcaaat catcttgaac attaaaggct tagcgctgaa
    32341 aatgtatggc caagaagaaa aaacccttga aatcgatatt accggacctg ctacagtaac
    32401 tgctggcgat attatcgttg atagtgatgt agaaattctt aacaaagata tgtacatctg
    32461 tagtgtctct gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta
    32521 tgttcaagca gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc
    32581 aatctacaca cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg ttggacaccg
    32641 tgaagatttt gataaattga cgatggagat ttggacagat ggttcaattg aaccgttgga
    32701 agcaatgagt ttgtctgcga aaatcatgac tgaacatttg gatatttttg tgaacttaac
    32761 tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat
    32821 gttggaaatg acgatcgaag agttagactt gtctgttcgc tcttacaatt gtttgaaacg
    32881 tgcaggaatc aatactgtac aagaacttac aaataaatct gagcctgaaa tgatcaaagt
    32941 ccgtaactta ggacgtaaat cattagaaga agttaaagca aaattgcacg acttaggttt
    33001 aggtttacgt aaagaagatt aaaacctttt acgaaggagg gaaaccaacg tgagttatcg
    33061 taaactagga cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa caactgattt
    33121 attaatcaac gaacgtattg tgactactga agctcgtgcg aaagaagttc gctcaactgc
    33181 agaaaaaatg atcactttag gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc
    33241 tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc
    33301 agctttacaa aaactgttca acgatattgc acctcgttac gcagaacgtc aaggcggata
    33361 cacacgcatt ttgaagacag aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga
    33421 acttgtctaa aatcacacta ataacatcac tttatagatg attcttttag agtgttatga
    33481 tgttggaaga tatttttccg agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg
    33541 ggagtaagca ctcatcataa agtgtttttt tataccttca attacctgtc atgacagata
    33601 tgtttgaagg cataaaaaaa cattttaagc gagaaataat tgaaaatttt gtcttttttt
    33661 gatagtgtta gtaggaactt taggatgaag aaggagtaat catggagcca attattgaat
    33721 tagaaaagat aaattataaa taccagccag atgatcttcg ccccgcattg aaagatgtct
    33781 cctttacaat tgataaaggt gagtggatcg ctattattgg acacaatggt tcaggaaaat
    33841 cgacacttgc caaaacgatc aatggattgc ttctgccaga atcagggatc gtgaaagtgg
    33901 gaaatcaaat attggatgaa gaaaatattt ggacgatccg acagatggtt ggaatggttt
    33961 ttcaaaatcc agacaatcag tttgttggtt cgacagtaga agatgacgtt gcttttggtc
    34021 ttgaaaatca agggattccc agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga
    34081 aagttagaat ggctgagttt gcttcacgcg agcctgctcg tttatcagga gggcaaaaac
    34141 aacgtgttgc cattgccggc gtggttgcct tacgacctga tatcattatt ttagatgaag
    34201 caaccagtat gctagatcca gaaggtcgtg aagaagtgat ctctacgatc aagaagatca
    34261 aagaagaaag tcaattgacc gttatatcta tcactcatga tattgatgaa gcagctaatg
    34321 ccaatcggat actagtgatg agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa
    34381 ttttttctgc tggtcctgaa ctgatcgact taggtctaga tctaccattt cctgaaaaac
    34441 tgaagagcgc actgaaagaa cgtggggttg atgtgcccag tgaatatatg acagaagaaa
    34501 ggatggtgga ttggttatgg acatccgttt tgaacaagta gacttcactt atcagccgaa
    34561 tacaccattt gaacaaagag ccttatttga catcaatatg acgatcaaag aaaacagtta
    34621 tactgcatta gtagggcata ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc
    34681 gcttgttaag ccaacaagcg gtacagtcca tattggagaa cgagatattc agccggatac
    34741 ggataataag aatttaaagc ccatccgaaa aaaagttggc atcgtctttc agtttccaga
    34801 agcacagcta ttcgaagaaa cggtagcaaa agacattgct ttcggtccta aaaactttgg
    34861 tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca ttagaacaag ttgggctgga
    34921 tgaaagctat ttggaacgtt cgccatttga actttcagga gggcaaatgc gtagagtagc
    34981 aatcgctggt gtgcttgcca tgaggccgga agtacttgta ttggatgaac caacggcagg
    35041 acttgatccc cagggtagaa aagaaatgat ggagatgttt tggcgtcttc ataaggaaca
    35101 gcagatcact attgtattag ttacgcattt aatggatgat gtagccaatt ttgcagatta
    35161 tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg gagccgcaag aggtctttca
    35221 aaacattgaa tggctaaaag aaaaacaact aggcgtacct actgccaccg agtttgcaga
    35281 agaattaatg gctaaaggga tgaactttgc taccttgcct ttgacagctg aagaattagc
    35341 agacgctatc gtccattatg ctggaggaaa tgcccatgat gaataaattg atatttggtc
    35401 gttatatgcc tggagattcc ttgatccatc gattggatcc ccgagctaaa ttattagcca
    35461 gtttttattt tatcggaatc atttttttag caaataattg ggaaacgtac cttttgttgg
    35521 catttttcac attactttct gtcttcttgt caaaagtaga tttgaaattt tttatccgag
    35581 gcattcgccc attgatctgg ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa
    35641 gtggcggaga aacttattgg agttggggtg ttttccatat cactgaattt ggtgtgatga
    35701 acggcctctt tattttctgt cgattcgtgt tgatcatttt tatgtcgact ctactcacgc
    35761 ttactacacc gcctttggaa ttatcggatg cgattgagta cttgcttcgt ccattgaaag
    35821 ccgtacggtt cccagtccac gaaatttcat tgatgctgtc gattgctctt cgttttgtgc
    35881 ctacattgat ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt
    35941 ttggtgaagg aaacctagtt cagaaaatga aagcagtcat tcctttgctg attcctttat
    36001 ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc tatggaagca agaggctaca
    36061 aaggtgggga aggaagaacg aaatatcgtg tattgcattg gcataataga gacacgatgg
    36121 ttatcattgc ttttgtatta ttaactgtgc tactgatctt tttgagagga taaacgttca
    36181 gtgggccgct atctattctt ttcttgctga tctgtttgca agaaaggatg cgagcagtgt
    36241 atgacaagaa atattagggt ctgtggcaga ctaaggtcac aggcctcttt cttttatctg
    36301 aaaagaaaga acgaaaaaat tttataaaaa aatcattatt ccagataacc ttttaggaac
    36361 aaaggagaga aaaacatggt acgttacaaa gcaatcattg catacgacgg aacaaatttt
    36421 aatggattcc aaaaacagcc aaatgggcgt actgttcaag aagaagtaga aaaaacatta
    36481 caaaaaatgg caaatggaaa agaaatcacg gtctttggtt cgggaaggac agatgccgga
    36541 gtacatgcga ttgggcaagt aatccatttt gattatccag aggagcgtcc gttagaacga
    36601 atgcgtttcg cgctagatac ccagtcacca gaggatattg ctgtaagaca agtagaaatt
    36661 gtttcagaag acttccacgc aagatatttg gtcaaagaaa agacgtatca atttcgagtg
    36721 gatataggga aaccacgcag tccttttcga cgccattatg caagttattt tccttaccct
    36781 cttgatttgg aaaaaattca gcgggcgctt cctgatttat tagggactca cgatttcacc
    36841 tctttttgtg catcaggaag ctcgatagag gataaagtac gtacgatcta cgaggctaag
    36901 atggaggtaa atgaaacaaa agacgaattg ttgtttacat ttaggggaaa tggcttcttg
    36961 tataagatga tacggatctt agtaggaacg cttttgaaaa ttggcaatgg ccgattgcct
    37021 gaagatagta tcccagatat tattgcgaaa aaagatcgta atgcagcagg acctacagca
    37081 catcctgaag gactttattt gtatgaagtg aaatatgaat aagaggttgt gacagaagtg
    37141 gtcagcttca agaaataaga aggaatttac gaaaattact tctcaaaatt ttgcaaaatt
    37201 tcggcttatt ttcgaaggag ctacttctgg aacaccgtga atcagaggtt gtgaaaaagc
    37261 tgccctgcat caagtaataa gaacaaccaa acaaaaatag ctcgtcatat ttttcgtttg
    37321 gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa
    37381 tcagcgtttt gacctgagta ttagagaaac aaacggaaaa atagttcctc atattttgcc
    37441 gtttgttgag ctaataccga aggtcagctg attgaacacc gtttaagaaa gaggtcgtga
    37501 gcctgtcatt taactctgag tcacaagtaa caatttcaaa aaacagcttc tcatctttga
    37561 tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg ttacctattt
    37621 tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttaatgag gacagtcgga
    37681 acaggtttgt catttttata tttaaatggc acaaattttt ctaattattt gtcatttagc
    37741 gctgaaaatt tttgctattt atctaaaaaa gtgataacta cttaagatga agcagaatat
    37801 ttacgccata cggcatacaa ccgcgaaaaa tataaaaaaa agaaaacgat tgaacgcgtt
    37861 tttgcagtcg caaaggaaaa gcatggaatg cgctacatca agtatcgaga cttggaaaac
    37921 aatcaggtgt atacgctgat tgtttttgct gcaatgaatc tgaaaaagtt gtccacttgg
    37981 cagttggaac atatgtcttc atatctttta ttatacaaaa atagcaaaag caccagtcta
    38041 cactcttagt aaagaggaaa ctgatccttt tgtctacatt cttaaagtac ttaaactaag
    38101 tactttattt tactctttag gattgataca gtagcatcta tccctttatt aataatcttt
    38161 tattttttta aataaagatg ttgtctccat cttcaggcac taaaactttt tcggaaattc
    38221 ctttttcttt attgaaagac tttaattctt ctcttgacag tccccaatgg tttacagctt
    38281 ccatatgaac ggcaataatt gtggaatctg gtattgcttt atgcaattca tataaatcgt
    38341 ctttccccat aataagagat tctccaacta aaaaatgatt atctccagca tttgctacta
    38401 tgatttctgg attatactga tcaatagttt gtttaacagc ttcataccaa actgtatctc
    38461 ctgtaaagta tagtgtcttc tcagtcgaat gtttgaaaac aataccgcaa acattaccta
    38521 cataattcaa gatttctcct ctgccatgtt tgccttttgt tttaaccaat tgtatagatt
    38581 caaaaactgt gtcttctcgt agtacctcca catttttaaa accatatagt cgaatttcat
    38641 tcgcatcatt ttcatcttgc acaaataatt ttatttcttt aggtaggacc tctttcgcta
    38701 tatcgtccca atgatctaga tgtaaatgtg ttacaataac agcatcaata ccgttgataa
    38761 tatcttcgac tggcattggc aaatcaacta gaggattcat ttgatctttt cttggtgatg
    38821 gaaaaggacc tttcgaaata aatggtggaa gtgttccttt ttccgctaac attggatcaa
    38881 ttaaaaattt tttacctcca tagtctaaga tagctgttgc attacgaatg tgatgtatat
    38941 tcataaacgt tgtctccttt ttgttgatat gaaatatttt tagtataatt caattccata
    39001 caataaataa accgaacaaa aaaagtcttt tactattttt acttaattta tgaaaggttg
    39061 tttaccatgt tagaccaaac agatataaat atcattgagg aactatcaaa aaatagtcga
    39121 atcacaatga aagaacttgg agaaaaagta catttgactg gacccgctgt ctcggcgaga
    39181 gtgacaaaac tagaggaaag tggagttatt gaaaattata cagtaa
//
LOCUS       DAYYAG010000010        37318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000010 DAYYAG010000000
VERSION     DAYYAG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.010"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHARQ_000491"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHARQ_000491"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8408943.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHARQ_000492"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHARQ_000492"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8408944.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHARQ_000493"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHARQ_000493"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8408945.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHARQ_000494"
     CDS             2179..2499
                     /locus_tag="ACHARQ_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8408946.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHARQ_000495"
     CDS             complement(2611..3468)
                     /locus_tag="ACHARQ_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8408947.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHARQ_000496"
     CDS             complement(3576..4880)
                     /locus_tag="ACHARQ_000496"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8408948.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHARQ_000497"
     CDS             complement(4877..5386)
                     /locus_tag="ACHARQ_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408949.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHARQ_000498"
     CDS             complement(5561..7696)
                     /locus_tag="ACHARQ_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8408950.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHARQ_000499"
     CDS             complement(7779..9434)
                     /locus_tag="ACHARQ_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8408951.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHARQ_000500"
     CDS             complement(9436..9750)
                     /locus_tag="ACHARQ_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8408952.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHARQ_000501"
     CDS             9920..10180
                     /locus_tag="ACHARQ_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8408953.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHARQ_000502"
     CDS             10194..10508
                     /locus_tag="ACHARQ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8408954.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHARQ_000503"
     CDS             complement(11238..11792)
                     /locus_tag="ACHARQ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8408955.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHARQ_000504"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHARQ_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHARQ_000505"
     CDS             complement(14153..14869)
                     /locus_tag="ACHARQ_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8408956.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHARQ_000506"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHARQ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8408957.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHARQ_000507"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHARQ_000507"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8408958.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHARQ_000508"
     CDS             complement(18339..19799)
                     /locus_tag="ACHARQ_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8408959.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHARQ_000509"
     CDS             complement(19789..21084)
                     /locus_tag="ACHARQ_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8408960.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHARQ_000510"
     CDS             complement(21416..22093)
                     /locus_tag="ACHARQ_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8408961.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHARQ_000511"
     CDS             complement(22123..23214)
                     /locus_tag="ACHARQ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8408962.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHARQ_000512"
     CDS             complement(23315..23749)
                     /locus_tag="ACHARQ_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8408963.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHARQ_000513"
     CDS             complement(24333..25790)
                     /locus_tag="ACHARQ_000513"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8408964.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHARQ_000514"
     CDS             complement(25787..27682)
                     /locus_tag="ACHARQ_000514"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8408965.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHARQ_000515"
     CDS             complement(27740..29515)
                     /locus_tag="ACHARQ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8408966.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHARQ_000516"
     CDS             complement(29565..31061)
                     /locus_tag="ACHARQ_000516"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8408967.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHARQ_000517"
     CDS             complement(31255..32877)
                     /locus_tag="ACHARQ_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8408968.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHARQ_000518"
     CDS             complement(33205..34518)
                     /locus_tag="ACHARQ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8408969.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACHARQ_000519"
     CDS             complement(34763..34987)
                     /locus_tag="ACHARQ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408970.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACHARQ_000520"
     CDS             complement(35007..35429)
                     /locus_tag="ACHARQ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8408971.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACHARQ_000521"
     CDS             complement(35571..36281)
                     /locus_tag="ACHARQ_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8408972.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACHARQ_000522"
     CDS             complement(36299..36796)
                     /locus_tag="ACHARQ_000522"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8408973.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACHARQ_000523"
     CDS             complement(36833..37285)
                     /locus_tag="ACHARQ_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8408974.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT        10524 a         8032 c         6279 g        12483 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactag
//
LOCUS       DAYYAG010000011        35780 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000011 DAYYAG010000000
VERSION     DAYYAG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.011"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..1184
                     /locus_tag="ACHARQ_000524"
     CDS             114..1184
                     /locus_tag="ACHARQ_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8408975.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1199..1867
                     /locus_tag="ACHARQ_000525"
     CDS             1199..1867
                     /locus_tag="ACHARQ_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8408976.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1887..2465
                     /locus_tag="ACHARQ_000526"
     CDS             1887..2465
                     /locus_tag="ACHARQ_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8408977.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2866..3777
                     /locus_tag="ACHARQ_000527"
     CDS             2866..3777
                     /locus_tag="ACHARQ_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8408978.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4155..5072)
                     /gene="rnhC"
                     /locus_tag="ACHARQ_000528"
     CDS             complement(4155..5072)
                     /gene="rnhC"
                     /locus_tag="ACHARQ_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8408979.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5274..5711
                     /gene="zapA"
                     /locus_tag="ACHARQ_000529"
     CDS             5274..5711
                     /gene="zapA"
                     /locus_tag="ACHARQ_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8408980.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5769..6317
                     /locus_tag="ACHARQ_000530"
     CDS             5769..6317
                     /locus_tag="ACHARQ_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8408981.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6432..8792
                     /locus_tag="ACHARQ_000531"
     CDS             6432..8792
                     /locus_tag="ACHARQ_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8408982.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8879..9193
                     /gene="trxA"
                     /locus_tag="ACHARQ_000532"
     CDS             8879..9193
                     /gene="trxA"
                     /locus_tag="ACHARQ_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8408983.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9375..11153
                     /gene="uvrC"
                     /locus_tag="ACHARQ_000533"
     CDS             9375..11153
                     /gene="uvrC"
                     /locus_tag="ACHARQ_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8408984.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11298..12197
                     /locus_tag="ACHARQ_000534"
     CDS             11298..12197
                     /locus_tag="ACHARQ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8408985.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12190..13452
                     /locus_tag="ACHARQ_000535"
     CDS             12190..13452
                     /locus_tag="ACHARQ_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8408986.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13613..15511
                     /locus_tag="ACHARQ_000536"
     CDS             13613..15511
                     /locus_tag="ACHARQ_000536"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8408987.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15841..16560)
                     /locus_tag="ACHARQ_000537"
     CDS             complement(15841..16560)
                     /locus_tag="ACHARQ_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8408988.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16750..16797
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16897..17229
                     /locus_tag="ACHARQ_000538"
     CDS             16897..17229
                     /locus_tag="ACHARQ_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8408989.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17216..19219
                     /locus_tag="ACHARQ_000539"
     CDS             17216..19219
                     /locus_tag="ACHARQ_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8408990.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19238..19708
                     /locus_tag="ACHARQ_000540"
     CDS             19238..19708
                     /locus_tag="ACHARQ_000540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8408991.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19729..20313
                     /locus_tag="ACHARQ_000541"
     CDS             19729..20313
                     /locus_tag="ACHARQ_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8408992.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20328..21314
                     /locus_tag="ACHARQ_000542"
     CDS             20328..21314
                     /locus_tag="ACHARQ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8408993.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21304..21615
                     /locus_tag="ACHARQ_000543"
     CDS             21304..21615
                     /locus_tag="ACHARQ_000543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8408994.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21637..23418
                     /locus_tag="ACHARQ_000544"
     CDS             21637..23418
                     /locus_tag="ACHARQ_000544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8408995.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23411..24787
                     /locus_tag="ACHARQ_000545"
     CDS             23411..24787
                     /locus_tag="ACHARQ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8408996.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24794..25429
                     /locus_tag="ACHARQ_000546"
     CDS             24794..25429
                     /locus_tag="ACHARQ_000546"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8408997.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25459..26814
                     /locus_tag="ACHARQ_000547"
     CDS             25459..26814
                     /locus_tag="ACHARQ_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8408998.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(27006..28400)
                     /locus_tag="ACHARQ_000548"
     CDS             complement(27006..28400)
                     /locus_tag="ACHARQ_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8408999.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28492..29463
                     /locus_tag="ACHARQ_000549"
     CDS             28492..29463
                     /locus_tag="ACHARQ_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8409000.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29460..30242
                     /locus_tag="ACHARQ_000550"
     CDS             29460..30242
                     /locus_tag="ACHARQ_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8409001.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30411..30782)
                     /locus_tag="ACHARQ_000551"
     CDS             complement(30411..30782)
                     /locus_tag="ACHARQ_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8409002.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30916..31359
                     /locus_tag="ACHARQ_000552"
     CDS             30916..31359
                     /locus_tag="ACHARQ_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8409003.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31402..32211)
                     /gene="yidA"
                     /locus_tag="ACHARQ_000553"
     CDS             complement(31402..32211)
                     /gene="yidA"
                     /locus_tag="ACHARQ_000553"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8409004.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32230..33597)
                     /locus_tag="ACHARQ_000554"
     CDS             complement(32230..33597)
                     /locus_tag="ACHARQ_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8409005.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33581..34411)
                     /locus_tag="ACHARQ_000555"
     CDS             complement(33581..34411)
                     /locus_tag="ACHARQ_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8409006.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34574..35011
                     /locus_tag="ACHARQ_000556"
     CDS             34574..35011
                     /locus_tag="ACHARQ_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8409007.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            35087..35707
                     /gene="rpoE"
                     /locus_tag="ACHARQ_000557"
     CDS             35087..35707
                     /gene="rpoE"
                     /locus_tag="ACHARQ_000557"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8409008.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        11690 a         6066 c         7689 g        10335 t
ORIGIN      
        1 aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc gccaaaaatt
       61 tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt taaatgtttt
      121 tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca ggggtgatgt
      181 ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta gctcaagata
      241 atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa gatgcaaatt
      301 caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa ggtgggaaag
      361 tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca gaagaaggaa
      421 aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc aatcttgttg
      481 aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg aaagaagaat
      541 atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg tttaaactaa
      601 ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca accatcaatg
      661 cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg attaacgcaa
      721 tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc aaaatttcta
      781 aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga tttatgatcg
      841 gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta ctgacgatgc
      901 aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga tttattgcgc
      961 gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt ggtctattag
     1021 gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc aattggctgt
     1081 tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca ctattttctg
     1141 ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga ggaagaacat
     1201 gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc aagtagttaa
     1261 agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa tcatcggtcc
     1321 ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga cgcctagcag
     1381 tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg aacgcgttaa
     1441 actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc cttttctgac
     1501 agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc aaaaagaagc
     1561 aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc cagaagaact
     1621 atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg atccgtccat
     1681 tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag aagtagtcga
     1741 tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta ctcacgatac
     1801 acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat tgaaagaaaa
     1861 agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat acacagcaaa
     1921 aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc acttcaacaa
     1981 gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat ttcggtgata
     2041 aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga aaagctttga
     2101 gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa atggcgcagt
     2161 ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt gaaagcaatc
     2221 tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat cgtgaacctt
     2281 ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca aaagaaatcg
     2341 cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa agacctgtgg
     2401 aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta ttaggagaag
     2461 cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat gacgaagatc
     2521 gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt tattctctca
     2581 tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt tttaatttaa
     2641 taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta gatgaatcac
     2701 cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc attttagcag
     2761 taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact taagctgatt
     2821 ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa aaaaataatt
     2881 ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga tgtttttggt
     2941 caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac aaagacaaag
     3001 gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa tgaccgtaca
     3061 ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg agagctggcg
     3121 gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg ctatactgga
     3181 tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa accggaagag
     3241 ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac tggggcatta
     3301 agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat aaaagtaaaa
     3361 aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga tgaattagta
     3421 cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt tatcagttta
     3481 cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata ttattactat
     3541 ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct gcctctggat
     3601 tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca accttctatt
     3661 ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt aacagacagt
     3721 tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt tcagtaataa
     3781 ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg cttattatcg
     3841 cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg ttcaactctg
     3901 agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
     3961 accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca acgttttgtt
     4021 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
     4081 ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt gacatgactt
     4141 ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt ggcaaaatga
     4201 agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt tgccgctact
     4261 tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc tgatgctttg
     4321 tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac agctaaatga
     4381 tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt ttctaaacgc
     4441 gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc ttctggtttt
     4501 tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc
     4561 cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa gacaagcagt
     4621 ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct atcttttaac
     4681 tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg tttcacataa
     4741 gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc actacctaaa
     4801 acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg cttggcttgt
     4861 ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc ttgaaacatc
     4921 acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc aaagctagta
     4981 taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg actaagttgg
     5041 tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt atacataaaa
     5101 ctataccgca actcacgact tttcgttttc taagagattg cgagacatag cattttttta
     5161 aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg tgaaagaacc
     5221 aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt cgtatggcgc
     5281 acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt atcggacggg
     5341 aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta gcagaattga
     5401 aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct gtcaatgcgc
     5461 tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa acagcagaat
     5521 taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa
     5581 tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat accattaaaa
     5641 atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt aaacaaaaat
     5701 cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag aagagaaagg
     5761 aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt ttttctggag
     5821 cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg tcatttattg
     5881 ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt ccatacccag
     5941 ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt cacctagatg
     6001 aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg ctgcttaccc
     6061 gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga caggtagact
     6121 ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt ttgattttgc
     6181 agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag catagtttcg
     6241 ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag ctttgggtaa
     6301 cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg tacagagtag
     6361 tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat agtgaaagat
     6421 aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa gtcaaacaga
     6481 tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa ttggtaccag
     6541 tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga ttgaaagtgc
     6601 aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc catatgaaac
     6661 ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc agagtattgt
     6721 ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa atcgaatttg
     6781 cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc cgccgtttaa
     6841 aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag ttaaagagca
     6901 taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat gggatcgtcc
     6961 gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat gaccgctacg
     7021 ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac gatcaaagtg
     7081 cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat aatcgtttgc
     7141 gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa ctttctgctg
     7201 aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt aaaatggatc
     7261 tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg ggaatcagtg
     7321 aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag gaaaaagtag
     7381 tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca ggacctaaca
     7441 caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg gggcaagcag
     7501 gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa gtatttgcgg
     7561 atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct catatgacga
     7621 acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt gacgagctcg
     7681 gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta gatgatcttg
     7741 gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa gtttatggat
     7801 ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca ttaagtccaa
     7861 cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc tctagccgat
     7921 taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat gagagccaag
     7981 atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag accgaatatc
     8041 tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg aaagaagctt
     8101 acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa aaagcaaatg
     8161 aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt cgtaaaatgc
     8221 aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca aagacccaat
     8281 tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag aaggcgaaag
     8341 aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc caaagaggga
     8401 cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta aaaatgaatg
     8461 tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa cgagttatcc
     8521 atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt cgaggaaaac
     8581 gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt ctagctggtt
     8641 atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag ggaattactg
     8701 attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca aaccaaggcg
     8761 gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc agatggttcg
     8821 ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg tctttgatat
     8881 gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac ttgtattgat
     8941 tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt tggatcagtt
     9001 ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg atgaaaatcc
     9061 agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga aaaaagatgg
     9121 tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc aaatgatcga
     9181 tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa tccagttccg
     9241 tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt gagtataatt
     9301 acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta attgaatatg
     9361 aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt cctgatcagc
     9421 ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc aaagccaaaa
     9481 tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa acggaacggc
     9541 ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc gaagcattgc
     9601 tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg ttaaaggatg
     9661 ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg gtgattactc
     9721 gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc ggtgcggcga
     9781 atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa cctagtcaga
     9841 cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat tatttcgacg
     9901 tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta aacggaggac
     9961 acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa aacatggagt
    10021 ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc atgactcgcc
    10081 aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg attgataaag
    10141 gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa cgagatgttt
    10201 caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc ggtcagtttt
    10261 atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg atcgacaagc
    10321 ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga gaaaagaaaa
    10381 aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg tttgatctga
    10441 ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac gcgatgaata
    10501 tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg acgaatcctg
    10561 tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat cggaaatata
    10621 aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc atctatcgca
    10681 gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg atcgatggag
    10741 gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga atcgatattc
    10801 ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta ttcggtacag
    10861 atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc cagcgaatcc
    10921 aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt aaaaacagtt
    10981 ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa atgctgctta
    11041 aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta caagaaatcg
    11101 gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa tgaggaaagg
    11161 gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa gagcgtttac
    11221 gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat gtgatttctt
    11281 gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt gggaactaca
    11341 cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg ctaattggcc
    11401 aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg acacaagacc
    11461 aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat gtcatcggtt
    11521 atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa ctgctttatt
    11581 ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac ttttggatgg
    11641 aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct aaaggaaatc
    11701 agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc attttggatg
    11761 agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga atattggaat
    11821 tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat gtagaaaaaa
    11881 tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga aaagtacatg
    11941 agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta agccaacagg
    12001 aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat acgttagaga
    12061 ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact cagttcggct
    12121 acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa ttgaaagcag
    12181 gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa aaatgttaag
    12241 tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat catttatgta
    12301 gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt ggctgataat
    12361 gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt caaagcagtt
    12421 gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc gtatttagta
    12481 gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc attaggacaa
    12541 agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct tcgagcgagt
    12601 caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag tttgactcga
    12661 caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa ctcgacagtc
    12721 caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt gacgtatgcg
    12781 cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga agtgatcctg
    12841 tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt acttgttgca
    12901 ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca attcaaagat
    12961 atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat tggaccattc
    13021 ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt tttagcagct
    13081 ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt gccagtgact
    13141 tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga tccaaacaat
    13201 atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat gccgattcgc
    13261 ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt acttgtagcc
    13321 gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt actcgtttat
    13381 agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag gaatgaacga
    13441 aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt gttcccagct
    13501 tctttttgta tagacctatt ttatttattt tactatattg gatatatatt cattgaaaat
    13561 agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt taatggaccg
    13621 ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca cggaaatcat
    13681 caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga gtgatataca
    13741 tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa
    13801 gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat tcactatttt
    13861 tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag ataaatcgca
    13921 gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata cccggtcaaa
    13981 agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga
    14041 tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac ttgttttgat
    14101 gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat ctgtcatcga
    14161 tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca aagtgatgga
    14221 tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata ttttgtggat
    14281 tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg cagcacgcta
    14341 taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat tgttttcttt
    14401 tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta aaaatctatc
    14461 cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc ttcaattcaa
    14521 atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc gtaaattgct
    14581 ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct atgagttgaa
    14641 aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg aagaggaaca
    14701 aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc aaaaacatat
    14761 gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tgctgtttca
    14821 tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag accaatctta
    14881 tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg caaaagatct
    14941 gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg ggccattttc
    15001 gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca cagagcctca
    15061 tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt ggttcagtga
    15121 aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta atgggcacac
    15181 gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag tatttgtcat
    15241 tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt attcactatt
    15301 atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta ttgaacaaca
    15361 atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc aactaccacg
    15421 caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta cccagctcaa
    15481 aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat tgttccatag
    15541 actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac ggtgttcttc
    15601 tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg ttttttgaaa
    15661 ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat aaacggtgtt
    15721 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa gccattttca
    15781 caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc agaaagtcta
    15841 ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta cacggtgggt
    15901 aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc tttgaaccgc
    15961 ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa gatctttgac
    16021 tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc ttaacaaact
    16081 gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct gttgttcatg
    16141 ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt ccaaaacgac
    16201 attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt tcaatgcttc
    16261 caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta gctttggatg
    16321 aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa taagtttgtc
    16381 gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa taagaggcaa
    16441 tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca cttgatttcc
    16501 ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga ttggtttcat
    16561 ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt aaactatgta
    16621 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaaatttcgt
    16681 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
    16741 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
    16801 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
    16861 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
    16921 gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact gcggcagcag
    16981 ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac aaactatcga
    17041 caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga attagaaaaa
    17101 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agatcaatat
    17161 caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga aacttatggc
    17221 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
    17281 acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa atagaacaaa
    17341 caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag aagcaaaatt
    17401 aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca tacaacacca
    17461 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagattt
    17521 ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc ttgatttaaa
    17581 agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg aagaatggct
    17641 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
    17701 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac tgagtaagct
    17761 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
    17821 tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg ggttcaataa
    17881 agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca agcagcaatt
    17941 atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt gcagtggcta
    18001 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gagaaaaaat
    18061 aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg tagatgcaga
    18121 agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag atatatacgt
    18181 cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
    18241 ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
    18301 agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
    18361 cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc aaaaactact
    18421 tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct cgataccatc
    18481 gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa aagaactgtt
    18541 tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
    18601 tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg cagcaaagga
    18661 aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct ggcaagggat
    18721 cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata cagcatttgt
    18781 ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg ttccgattat
    18841 ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt acggactgac
    18901 cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta tttcaggcgg
    18961 aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg ctcgattcag
    19021 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgctaag
    19081 tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt tttatacagg
    19141 aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcacaa
    19201 gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact taattagtca
    19261 aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt tttcagggat
    19321 cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga caacaagtca
    19381 acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc aaggtttgta
    19441 tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt cagttgtaca
    19501 aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt tttcaggtat
    19561 tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac ctgaacatgc
    19621 tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag gtttcgttat
    19681 ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
    19741 gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc gttagaacaa
    19801 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt tgaaacagaa
    19861 catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg tcagctaagg
    19921 aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttaaga
    19981 cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga acaaatccag
    20041 tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt cattgctgga
    20101 gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa tgaacttccc
    20161 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
    20221 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
    20281 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
    20341 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
    20401 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
    20461 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
    20521 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
    20581 cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg acaaatcaaa
    20641 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
    20701 ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata cgagaagtac
    20761 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
    20821 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
    20881 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
    20941 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
    21001 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
    21061 atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta aagcttgaac
    21121 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
    21181 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
    21241 tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg gtgagacaag
    21301 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
    21361 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
    21421 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
    21481 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
    21541 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
    21601 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
    21661 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
    21721 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
    21781 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
    21841 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
    21901 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
    21961 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
    22021 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
    22081 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
    22141 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
    22201 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
    22261 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
    22321 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
    22381 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
    22441 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
    22501 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
    22561 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
    22621 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
    22681 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
    22741 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
    22801 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
    22861 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
    22921 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
    22981 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
    23041 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
    23101 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
    23161 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
    23221 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
    23281 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
    23341 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
    23401 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
    23461 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
    23521 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    23581 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    23641 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    23701 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    23761 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    23821 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    23881 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    23941 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    24001 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    24061 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    24121 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    24181 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    24241 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    24301 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    24361 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    24421 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    24481 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    24541 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    24601 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    24661 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    24721 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    24781 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    24841 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    24901 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    24961 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    25021 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    25081 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    25141 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    25201 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    25261 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    25321 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    25381 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    25441 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    25501 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    25561 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    25621 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    25681 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    25741 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    25801 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    25861 aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    25921 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    25981 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    26041 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    26101 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    26161 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    26221 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    26281 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    26341 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    26401 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    26461 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    26521 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    26581 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    26641 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    26701 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    26761 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    26821 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    26881 ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc
    26941 tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct ttatttatca
    27001 ataggttata attcatattt ttttattttt tcttttacga tacttagtcc agaagctaaa
    27061 atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg cgcacgtgta
    27121 gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt ctcaattatc
    27181 ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg attcgttggt
    27241 ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag acttaattta
    27301 gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac ttccacatct
    27361 aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct ttggacatgt
    27421 actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt cattccagct
    27481 aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt atgttgatag
    27541 taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc tttttcaatc
    27601 acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg attattacaa
    27661 gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat caatatatca
    27721 ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata taactgagcc
    27781 aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc tggagagaaa
    27841 agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga tagagattct
    27901 aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa ttgaaaattt
    27961 gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt actagttgat
    28021 tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag accaaaatca
    28081 atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac attgggagaa
    28141 ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc atatttataa
    28201 gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct ttgggagggt
    28261 aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg ttgaatcaac
    28321 gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa cgggtaattg
    28381 tcataagaat taataggcat tttctccact ccttaaaatt gtataccata caattttacc
    28441 attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc tatgaaaatt
    28501 attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca agtaatgaaa
    28561 aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt
    28621 ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt gggggagaac
    28681 agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta tcatttacct
    28741 tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa agcattgata
    28801 aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc aacagattac
    28861 ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca agcattgtcg
    28921 catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta cgatatttta
    28981 gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa aataacgttt
    29041 ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg tacgttgacc
    29101 tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct
    29161 gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga aacttctcaa
    29221 atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct tatagccatg
    29281 gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt agtttctgag
    29341 aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt tggtctagct
    29401 attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg ggctttaaaa
    29461 tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag
    29521 cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa attgccattg
    29581 aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt gagcttagat
    29641 ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca acagcatttg
    29701 ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact caaagcggta
    29761 atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt attatgccga
    29821 aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt aaagaagcct
    29881 acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct aaattgcagc
    29941 cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg acagcagaaa
    30001 gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc aatgaagatc
    30061 cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga atgaataaaa
    30121 caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt gaattaaaag
    30181 aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc tcgtttattt
    30241 gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta tagctacaat
    30301 ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt ctcattttaa
    30361 ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa ctatcctcgg
    30421 atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg cgcagtgcca
    30481 ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc gtgtaatttt
    30541 tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc atacgcacca
    30601 tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt caaataaaca
    30661 agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc aaatgccttt
    30721 tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg agccattttc
    30781 atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta aggaaatagt
    30841 tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat aaaactgtta
    30901 aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt tgctgattat
    30961 caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc agtaatcgag
    31021 gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga gacgaaagaa
    31081 gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat ttcttcttat
    31141 ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta ttataatcca
    31201 ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga aaagaccaat
    31261 aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg tagccaccga
    31321 tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag tagatcagct
    31381 ttctctcagt ttttttattt attacagtac atattttttg attgcttcag ctacaccgtg
    31441 ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa cggcatttcc
    31501 cattgctact cctagaccag catattcaat catggataag tcgttctcgt tatctccgat
    31561 agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa ctgccgcacc
    31621 tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga ccgttgtgta
    31681 tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt ctggttcgtc
    31741 gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg ttcgatattt
    31801 tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat ctcgattagg
    31861 cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc gtgccattac
    31921 ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt tacctgattt
    31981 cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt gaaaaaggtt
    32041 caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata aaacgatcga
    32101 aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta cttctctttt
    32161 ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca taatttcctc
    32221 caatatattt taatttattt caatcaatgc gccattacgg atatggctgg caaattcttg
    32281 ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca tttcttttgg
    32341 aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa gctgactaac
    32401 ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg tgcgatgtct
    32461 gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca cagcagtgta
    32521 ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga ctaattttga
    32581 atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa aacgtttagc
    32641 taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag tgcccaagac
    32701 cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa atggcagaag
    32761 taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt gtgcgcgatg
    32821 aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt acacttgcac
    32881 atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac tcatggaaaa
    32941 agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat caaatgttcc
    33001 atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat ctgcatcgat
    33061 ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga tcacactagc
    33121 taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt ctgtttctgg
    33181 agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat gttcaaatgt
    33241 atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag tcaccagccg
    33301 ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc gagaaaagat
    33361 atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac ggctatgttc
    33421 tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta gacgttggac
    33481 ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat agttatggac
    33541 tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg ttgtcataat
    33601 gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc cattgctcca
    33661 gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca gaatacagct
    33721 tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt tggtcaagca
    33781 gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt gtaccaaagt
    33841 cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt tcctgattac
    33901 cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc tgagcgatac
    33961 cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa gaatgggtga
    34021 tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg agcatttgct
    34081 cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc aaagaaatat
    34141 tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc actggagcgt
    34201 aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga acatccttca
    34261 tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc tggtgttctt
    34321 tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt ttttgataaa
    34381 tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat aaattcatat
    34441 tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt tttctgtaaa
    34501 aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta tgagaagtaa
    34561 aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat tgcgtacaaa
    34621 agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc aaatggtgac
    34681 aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg gcgatgagat
    34741 tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg caggagaaat
    34801 gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc gaacaccata
    34861 cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga aagaccgtcc
    34921 cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa aaatcggcga
    34981 atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga ttttttgtgt
    35041 ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg aaattagtgt
    35101 atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg cgatcttaga
    35161 acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa actatcttgg
    35221 gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact tgaatattga
    35281 cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt atgctatcga
    35341 ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata ctccacgtcg
    35401 ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga tcgattacaa
    35461 tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc tgtttgatga
    35521 cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag aaatcggagc
    35581 agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag atttaagtat
    35641 catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg actctgaaaa
    35701 agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct atcagaataa
    35761 ggaggtgtga cacgattttt
//
LOCUS       DAYYAG010000012        34960 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000012 DAYYAG010000000
VERSION     DAYYAG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.012"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHARQ_000558"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHARQ_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8409009.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHARQ_000559"
     CDS             complement(2020..2922)
                     /locus_tag="ACHARQ_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8409010.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHARQ_000560"
     CDS             complement(2919..3611)
                     /locus_tag="ACHARQ_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8409011.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHARQ_000561"
     CDS             complement(3654..4295)
                     /locus_tag="ACHARQ_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8409012.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHARQ_000562"
     CDS             complement(4333..4647)
                     /locus_tag="ACHARQ_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409013.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHARQ_000563"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHARQ_000563"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8409014.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHARQ_000564"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHARQ_000564"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8409015.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHARQ_000565"
     CDS             complement(5984..6916)
                     /locus_tag="ACHARQ_000565"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8409016.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHARQ_000566"
     CDS             complement(6913..7689)
                     /locus_tag="ACHARQ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8409017.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHARQ_000567"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHARQ_000567"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8409018.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKVIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHARQ_000568"
     CDS             complement(10933..12009)
                     /locus_tag="ACHARQ_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8409019.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHARQ_000569"
     CDS             complement(12014..13294)
                     /locus_tag="ACHARQ_000569"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8409020.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHARQ_000570"
     CDS             complement(13313..14239)
                     /locus_tag="ACHARQ_000570"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8409021.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHARQ_000571"
     CDS             complement(14252..15535)
                     /locus_tag="ACHARQ_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8409022.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHARQ_000572"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHARQ_000572"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8409023.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHARQ_000573"
     CDS             complement(16323..17234)
                     /locus_tag="ACHARQ_000573"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8409024.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHARQ_000574"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHARQ_000574"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8409025.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHARQ_000575"
     CDS             complement(17700..18398)
                     /locus_tag="ACHARQ_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8409026.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHARQ_000576"
     CDS             complement(18385..18771)
                     /locus_tag="ACHARQ_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409027.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHARQ_000577"
     CDS             complement(18918..20588)
                     /locus_tag="ACHARQ_000577"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8409028.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHARQ_000578"
     CDS             20771..21004
                     /locus_tag="ACHARQ_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8409029.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHARQ_000579"
     CDS             complement(21103..21534)
                     /locus_tag="ACHARQ_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8409030.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHARQ_000580"
     CDS             21613..22026
                     /locus_tag="ACHARQ_000580"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8409031.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHARQ_000581"
     CDS             complement(22064..23212)
                     /locus_tag="ACHARQ_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8409032.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHARQ_000582"
     CDS             complement(23334..23930)
                     /locus_tag="ACHARQ_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8409033.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHARQ_000583"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHARQ_000583"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8409034.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHARQ_000584"
     CDS             27160..27591
                     /locus_tag="ACHARQ_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8409035.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHARQ_000585"
     CDS             complement(27636..28985)
                     /locus_tag="ACHARQ_000585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8409036.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHARQ_000586"
     CDS             complement(29055..30218)
                     /locus_tag="ACHARQ_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8409037.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHARQ_000587"
     CDS             30347..30670
                     /locus_tag="ACHARQ_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409038.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHARQ_000588"
     CDS             complement(30853..32202)
                     /locus_tag="ACHARQ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8409039.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHARQ_000589"
     CDS             complement(32271..33224)
                     /locus_tag="ACHARQ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8409040.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHARQ_000590"
     CDS             complement(33326..34516)
                     /locus_tag="ACHARQ_000590"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8409041.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34960)
                     /locus_tag="ACHARQ_000591"
     CDS             complement(34716..>34960)
                     /locus_tag="ACHARQ_000591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409042.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9952 a         7641 c         6119 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggatta ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa
//
LOCUS       DAYYAG010000013        34896 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000013 DAYYAG010000000
VERSION     DAYYAG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.013"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..1617)
                     /locus_tag="ACHARQ_000592"
     CDS             complement(49..1617)
                     /locus_tag="ACHARQ_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8409043.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1621..2031)
                     /locus_tag="ACHARQ_000593"
     CDS             complement(1621..2031)
                     /locus_tag="ACHARQ_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8409044.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2028..2951)
                     /locus_tag="ACHARQ_000594"
     CDS             complement(2028..2951)
                     /locus_tag="ACHARQ_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8409045.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2948..3826)
                     /locus_tag="ACHARQ_000595"
     CDS             complement(2948..3826)
                     /locus_tag="ACHARQ_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8409046.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3813..5150)
                     /locus_tag="ACHARQ_000596"
     CDS             complement(3813..5150)
                     /locus_tag="ACHARQ_000596"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8409047.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5153..6256)
                     /locus_tag="ACHARQ_000597"
     CDS             complement(5153..6256)
                     /locus_tag="ACHARQ_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8409048.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6330..7838)
                     /locus_tag="ACHARQ_000598"
     CDS             complement(6330..7838)
                     /locus_tag="ACHARQ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8409049.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7804..8496)
                     /locus_tag="ACHARQ_000599"
     CDS             complement(7804..8496)
                     /locus_tag="ACHARQ_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8409050.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8518..9492)
                     /locus_tag="ACHARQ_000600"
     CDS             complement(8518..9492)
                     /locus_tag="ACHARQ_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409051.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9792..10664
                     /locus_tag="ACHARQ_000601"
     CDS             9792..10664
                     /locus_tag="ACHARQ_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409052.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11122..13320)
                     /locus_tag="ACHARQ_000602"
     CDS             complement(11122..13320)
                     /locus_tag="ACHARQ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409053.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13552..14544
                     /locus_tag="ACHARQ_000603"
     CDS             13552..14544
                     /locus_tag="ACHARQ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8409054.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14604..16025
                     /locus_tag="ACHARQ_000604"
     CDS             14604..16025
                     /locus_tag="ACHARQ_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8409055.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16018..17517
                     /locus_tag="ACHARQ_000605"
     CDS             16018..17517
                     /locus_tag="ACHARQ_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8409056.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKLIVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17556..18950
                     /gene="uxaC"
                     /locus_tag="ACHARQ_000606"
     CDS             17556..18950
                     /gene="uxaC"
                     /locus_tag="ACHARQ_000606"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8409057.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19109..19792
                     /locus_tag="ACHARQ_000607"
     CDS             19109..19792
                     /locus_tag="ACHARQ_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8409058.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19794..20630
                     /locus_tag="ACHARQ_000608"
     CDS             19794..20630
                     /locus_tag="ACHARQ_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8409059.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20641..22395
                     /locus_tag="ACHARQ_000609"
     CDS             20641..22395
                     /locus_tag="ACHARQ_000609"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409060.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22671..23012)
                     /locus_tag="ACHARQ_000610"
     CDS             complement(22671..23012)
                     /locus_tag="ACHARQ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8409061.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23080..25251)
                     /locus_tag="ACHARQ_000611"
     CDS             complement(23080..25251)
                     /locus_tag="ACHARQ_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8409062.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25462..26319
                     /locus_tag="ACHARQ_000612"
     CDS             25462..26319
                     /locus_tag="ACHARQ_000612"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8409063.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26390..27247)
                     /locus_tag="ACHARQ_000613"
     CDS             complement(26390..27247)
                     /locus_tag="ACHARQ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8409064.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27232..29934)
                     /locus_tag="ACHARQ_000614"
     CDS             complement(27232..29934)
                     /locus_tag="ACHARQ_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8409065.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29947..31236)
                     /locus_tag="ACHARQ_000615"
     CDS             complement(29947..31236)
                     /locus_tag="ACHARQ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8409066.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31386..32432
                     /locus_tag="ACHARQ_000616"
     CDS             31386..32432
                     /locus_tag="ACHARQ_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8409067.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32434..34587
                     /locus_tag="ACHARQ_000617"
     CDS             32434..34587
                     /locus_tag="ACHARQ_000617"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8409068.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        10242 a         7182 c         6293 g        11179 t
ORIGIN      
        1 ctaactagtg aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc
       61 ttttccatca tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc
      121 atgcactgct atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt
      181 ttgcggacct actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt
      241 ctctttcttt acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc
      301 ttctatttgt gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc
      361 attccaagta acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc
      421 catctgatta aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc
      481 cgtttgccga atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat
      541 cagacgtaaa aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg
      601 tctggcattc cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt
      661 ttcttgttgt acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct
      721 atctttaagt cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct
      781 tcccaaaata gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc
      841 tgacggggtt tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc
      901 actagctgtc agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc
      961 tcggcaaact gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata
     1021 atacccgtta cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa
     1081 tattttatag atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat
     1141 accgctggat ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt
     1201 ttttcccgtt tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat
     1261 tggttcggac caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt
     1321 aaacggacgg cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata
     1381 actgatttgt ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt
     1441 ggtcaaaata tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat
     1501 tcctggaagc cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat
     1561 attaggatct ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat
     1621 tcaggctcct tttctcattc ttactcgata taaactgaat gccccagctg cgaaataaaa
     1681 aaatgcaaaa ggaaacgcaa taaactgagt gccaatcaac aatatgaatc ctgttataaa
     1741 atacaaacca gtggcagttg ttaagtattt tcctttagca gtcaaaaaca atgctactgc
     1801 taaaaaagca agtgaaataa acaacacaac ggcaaacaga ccagtcatgt cctgtaataa
     1861 tccaaatgta ttttctaaag acaaagttga atgatctaca gcaagcgact gataactttt
     1921 tttaaactct gacgcagaca tattacttaa agtaactgct aacccgccaa agaaaatcaa
     1981 agatagactg ccaccgacca ttcctagcaa tagttcccat tttcttttca tttctgaacc
     2041 ccctgctttt ctaaatgtat ccattcaggt cttttgatcg atgtttcata attgttatta
     2101 cgttcagaga atttgacagt ctgttcattt ttcttgtcat cccaattatc ccaaccagtt
     2161 gggtgaacat gcccacctat cttacacgct tcaaataaga catttgcata cggacgccac
     2221 ggtcttccta aatagacgtt tctagtatca ggttctgcag tcaaggtaca gctttcaaaa
     2281 cgataccctg ttttttgatt tttcggcgta gatgctgctg tgatgtatcc aggattttta
     2341 ctggctaagc tatggatatg acactgatga aaaacggcat ctgccccacc aaatatgaaa
     2401 tccaccgttc cttcaatcca gcaatattca taggtctgac gacaatactc aggattggca
     2461 gaagccggtg tacaaaaagg agtaccatct ttttgcaaat caggcaacgg acccgtgcat
     2521 aaggtatcct gataaccaga tagtcgacag ttttttaaag atacgcgatc tcctgaattg
     2581 aataaagcaa cagcctgtcc taccttttct cctggacctg ctgtattttc gatgtgaaga
     2641 ttttctaata cgacattcga tccttctaag aaaaaagtag cacttcggaa agtccctaac
     2701 tggctaccgt caagatactc ctgttttgca aaacgattac cggagataac tactgttcct
     2761 gctcccacaa gtttaaaatt ggataatctg ctttctatat attcatcata tcttccttct
     2821 tttactatca agacctttgg atggctatcc ggcaggcctg ccaaataatc gattccttct
     2881 tggatagttt caaaatccgt cggggtatct atagcattct ttccaattat cagtttgtct
     2941 ttcatcctca taaatgctcc cttatgaatt gtttccaaat atttctcatt tcttgtgttt
     3001 ccatatgacc gcctgttaaa gaaaaagaag aaaagttttc tggactaccc attgattgat
     3061 actttttgtc cagccctttt ctcagcttca gtacgccttc gtccaagcac atcgtatcgt
     3121 tttttccaac taagctaaga cgagggcgcg gagcgatttt ttcttggatt gctaacgttg
     3181 tataagctgt caatagattt ggcacatagt aataaaaccc atgatgatct aggcggcgat
     3241 gtagcaaaag tatttcgatg tccacctgtg ccgctagatc aatacaaact ttgacacgag
     3301 aatccaatgc tgtcacccac cagcttaata agcctcccat agaaagacca atcgttgcga
     3361 gccgttgagt atctgtgtct tcccttgtct ccaaataatc gatcaagctg atcacatcat
     3421 aaatacgcat tccccataac gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt
     3481 cagaaatacc tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca gcatatccca
     3541 ttcctgtcag tacttctgca aaagatggag aagctaagta aggagcacca tggatcaatt
     3601 cacttttccc gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg
     3661 gtccttcttt ttctggataa gcatagaggg ctggtactgt ttccaattca tttaagtaca
     3721 attgatagta ttcaagcata aaaccgtttt gtttttctcg gtataatcgt tttccactag
     3781 gggtatgtct gctagatgga gcacctaaaa aatcattcaa ctgcataagc aaagttttta
     3841 cgaacaacca gttcatccat attttctgat tgatttgatt ggaatttctc aatactgatt
     3901 tctttagtat tttctaatag aaatgctggt ccctcgtgat tttcaatcga catctggtta
     3961 aaggaaacct tacccgcaaa gccaatataa aatccttgtc tgctcatttc ctctattcct
     4021 gtcatcatag caggaacacc gggaactgca tcttgggcca tcgagacatc gatattgtca
     4081 aatgtgattt cttgtatgaa ttgttccgct aatccataaa taaaacctgc agaagcatgg
     4141 acattccttg ccgtgatatt cgaaaaatgg atccttctga atgctggtgt ccgttcatct
     4201 atcggataag cctttttttc ccatacatac ggttctttcc ctcgcggacc acagaaataa
     4261 taaagattca gtataaatgg acacatcaca ttatccatga ctatattgga tactcgaata
     4321 tcttcaacga tcccgcctcg acctcgtcta gatttcaatc gtattcctcg atcagtttct
     4381 tggaagatac aatttgaaat ggtaatatta cgaatactac cactcatttc acttcctaat
     4441 acgacaccgc catgtccatg gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg
     4501 atcctttcgt acgtatcttc agtccctgcc ttgattgcaa tacagtcatc tcccacgtca
     4561 atatggcaat tactaatacg cacatttttg catgattctg gatcaattcc gtctgtattt
     4621 ggtgaatccg ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc
     4681 gggttgatcg tccaacttgg tgactggatc aatttaatat ctttcacagt aattcgatga
     4741 cagttatgga aactcatcaa tttaggacga ggataagcga gattatctgg ttcatttcta
     4801 aaagtatgcc accatttttt tccatttcca tctaatgtac cgaaaccagt gacagagatg
     4861 ttctctacat tttgagcata gatacaggaa gcataaactt ttcgatggac tccttcccaa
     4921 cgtgaatgaa caacaggata atcttttgga tcgtcagaga atttcagaac cgctcctgcg
     4981 cttaaatgaa gttcgacatt gctttttaaa aataaagctc ctgttagaaa ttcacctgct
     5041 ggaacaacta cggtcccgcc tccgtcactt gctgcagcat caatggcttg ttgtattgct
     5101 tcagtattca attcatcaat tgatgcgcca aatgtcaaaa tatcatacat atttataatg
     5161 cctccacttc aatcaatgct tgcaagaatg caccaacgcc tttttgatca ttacaaataa
     5221 ttggttcact gatgtagtaa gcatatgaac catctcgctg atctgctccg cctaaccctg
     5281 ctacttgaca gtttttattc aagttcagcc agccttcttt tgtttccaga acaaattcat
     5341 caagtaatcc ttgatgtgat ttcttcactt gtggaagcca ttcttctttg ttcagcagtc
     5401 ccaaacgtat tcctttagcc attgcgtaaa caaacatact actgccggaa gcttccagat
     5461 aatttccttt ccgttttgtt tctgttgtta cttgatacca tgtcccgctg ttttcttctt
     5521 gatatggaag taatgcagcc agtgtctttt ctagaatttc tttcagcaaa gaacaatctg
     5581 tttcttcatg aagtaattca taggtatctg ctgcagccat caaataccag ccaatcgatc
     5641 ggctccaaaa atgatgggat agacctgttt ttggatcagc ccatggttgg accttctttt
     5701 catcccatgc atgaatcagc aaatttgttt ttggatcgaa tagatgttta taactgattt
     5761 caaactgtct caaaacatct tggtaatctt tttcttcact aaaattctgg ataaattcag
     5821 cataaaaagg agaacccata tataagccat ccaaccaaat ttgataagga taaatttctt
     5881 tatgccaaaa agctccttcg gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt
     5941 ctgctgcttt tttatatttt tcttcgcctg tttctttgta caataaaaag aaaagctttc
     6001 ctgtatttac atggtcgata ttgtattctt caacgctgta gccacggatc gttccatctt
     6061 cttgcacgaa atagtcgata ttgtctttta tgaaatcaaa ataaattttt tcttgggttc
     6121 gttcccaaag taattggaac cctttaagta cgacgccata gtcataagac cattttcctt
     6181 tataccactt ttcttcatat aagcgaggtg ttctttccat gatacttttt gctaatttct
     6241 ttgaccagtt ttccattatt tgctctcctt taaaaattaa aataggattt gggacaaata
     6301 ccaagaagta tttgtcccaa tcgtctatat tactgtgccg ctgctttgta tgcttcctcg
     6361 tactcttgtg tgattcgatc gcctccttgt gctttccagt cttctacagc ttttttgaat
     6421 ccatcttcgt caagctgtcc catgatgtat tgcaccgtag catcaatgac gattttttca
     6481 atttctgatc ctacttcatt gttcgtcggt gaatccaatg gcacagaagg atccattaca
     6541 gcaaattctt cattttcttt gatcaattga ttagagaatt ctttttcagg gtctgtatct
     6601 ttgaaagaga acgtcacttc actaggacga gagcttgata acggttgcac ctcttgttgc
     6661 caaagatcca tatcgataaa ttcgatagaa ccatcatcat tcaattcata atgcgttcct
     6721 tcgataccgc cagtcatcaa tttatacaca tcttcttcta aaagatcatc aacaaattga
     6781 agtaatttct tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc tagcaagccg
     6841 ccaataccat ttccttctga ccaaacatga taaccgccgc cttcaccatc tgagatcttg
     6901 tttacaggca ctaattccat atcatcttga ataccttgac ccatcgtctt cagattcttg
     6961 atatccacca ttccagtgta gattccagtt cttccttgtg caaattgttc ttgctgatca
     7021 gtcttttggg taactgcaaa gtcttgtgcc aaatagccat tctcaaatag atctcttgaa
     7081 aaattcaaag ctttcatata ctcttctgta tcaaattctg gagtgaattt gccattatcg
     7141 tctacagccc agccatttgg tgctccaaaa taagaagtga aaagtttaaa gctggtataa
     7201 cgaatatcac taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc atttccatca
     7261 ggatcgtttt ctgtaaattg tttagctact tcataaagct catccatggt agttggaaca
     7321 tctagtccta gattatccaa ccagtctttt cgaatgacta agccagcacg agcgtagtct
     7381 ttttgtatcg gtacgccgta caaggcaccg tcgatactcg cagcttcgat tctatcttca
     7441 gaaatttgac ttaaattttt atagtctttt aaatattctg atacatccca gaatacgcct
     7501 gattttaacg cattgcggac agaagagttt ttcaacatcg caatttgaag tgtaacgata
     7561 tctcctagtt catcagaagc caatgcagtt gttaatcttt cttcttttga agcatcagga
     7621 atccagttga atgtcagttc cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct
     7681 gtaggcggtg aagcatgatg caaaatattt aaccaagtga ttttagcttt tccatcttct
     7741 gtctctgaag aactgttact tgaactgctg ccgcatgcgg caagtgctag aattcctgca
     7801 aaactaagac ctaattttac gagcgttgct tttttcataa attttctcct tcactgtttt
     7861 aataatattt ttgttttctt ctgttttttc tgtatatgag ttcttccatt ccaaatggca
     7921 aaatatagaa tttgctgtcc acgtgactgc ggaaaagaag agactgccac cgataaaggt
     7981 taaaacacct ggcattgttt ggatgatcat cagataaatc cctaccatac ttaatatcaa
     8041 cactaatgag taatgaagat gtgagaatcc gaataaaaat gccattttca ttttgaaaaa
     8101 atatcctttc caatcataat gagccatgat tgggaaaata tagagtgctg agagcacata
     8161 tatgaacgca gcaatataca aagcgatcct aagataccaa ttgtgtatga aagaaagatc
     8221 cacaagtaaa acaatcccga tggcgccata tatccagctc atcagcaagg tttcacgata
     8281 gttttcttta aaatatgtga agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag
     8341 ccattttcta atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa
     8401 acctgcgatg actaaaccgc cccacagaaa attcaaataa accaactgta aaatacgatt
     8461 tagccttccg tttattttat ctaatgactg cagcatatct ttccctctct ttctatctta
     8521 gtaaacgccg tcttctccta gctttttggc aatcttatta gctaaaatta ccataaacaa
     8581 tcctacgacc cctttaaaca atcccattgc tgtactgaaa cttaactgtc cattcttcaa
     8641 tccagctgta taaatatagg tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa
     8701 taacaagtac atatgctcga accctaagtc taatgtctga ccgattttta gaatcagcaa
     8761 ggtaatgatt actggacgaa ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc
     8821 tgctccatcc atttctgcag cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag
     8881 gtagatgatc gtagaccagc ccaactcttt ccagatcact tgtccgatat atactgtgcg
     8941 tgtccattca ggggatgtta gaaaactgat tttttcaaat cccattgttg caaggaattc
     9001 attcaatacc cctccgtcaa cattcaaaaa tacataagta atcgaaacaa tgattaccca
     9061 agacatgaag tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt
     9121 tacttcattt aatagtaaag acaaaatgat tggcatcgga aaaaacacga agatatttaa
     9181 tccgaacaag atcaacgtat ttcttaataa tgtaaagaat gtcggttctg taaacaaacg
     9241 aatgaaatgt ttgaaaccga cccaaggact tcccattatt cctaaaaatg gttgataatc
     9301 ttgaaaagca ataacgagtc cccccatagg aagataccta aaaatcaaga aataaaggac
     9361 tcccggcaga atcatcacat ataaaaattt attcgttttg attcctgcaa ttattttttt
     9421 tcgtcttctg agcttttctt cttttttcga tagcgtcagt tgaaatggtt cttgtttctt
     9481 tacactttcc ataagcccct ccttagtttt gtgaatgttt gctgcttatg acttcatgat
     9541 agataactat ctgaattacg tatataatca tttttaccgt aagtaaaaac gcttacatag
     9601 caatgatttt tgtgctatac aaaacgataa agtgtatttt ttcacaaata aactacgcat
     9661 agcaaacctt ctattttttt ctatctgaca cttagcatag gaaatgatta tatacgtaat
     9721 ctttcaccga tgataaattg aacatataga aacagaaaca tttaatttat tttgaggagg
     9781 agcctatcta tatgaaaaag aaagtctcta taggagaaag aagttttaca cttttcaaca
     9841 atacgtttct aattttgcta gctttgatct gtatcgttcc ttttttaaat atcatcgcta
     9901 catcttttgc ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt cctactacct
     9961 tttctttaga cgcgtatcgc tacattcttt ctacgccgac catttttaga gcacttgctg
    10021 tttcgattgg tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg
    10081 cctacggtct ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat
    10141 tttctatgtt gttcagcggg gggatgattc caacgttctt agttgttcgt tctctagggc
    10201 tggtcaattc ttactgggcc atgatcctgc ctgtggctgt taatgcaatg aatatgatca
    10261 ttatgagaaa ctttttccaa gccttgccgg atagtttgga agaatcagca aaaatggatg
    10321 gttgcacaga ttttggtgta tttttcaaaa ttatgctgcc attagcgtta ccgtctattg
    10381 ctactatctc gcttttttat gctgttactt actggaacac ttatatgact gctattttgt
    10441 atatcaacga ttcatctaaa tggccgatac agattctttt acgccaaatc gtcattgtat
    10501 caagtgggat gcaagcagaa agtagtgccg ttgatgtgat tccaccagca caaacagtta
    10561 aaatggctgt tatcgttatt gcaacagtgc ctatgctgat tgtttatcct tttgttcaga
    10621 aatattttgt caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca
    10681 aaatggtagt tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt tacgaaaatt
    10741 gcttcttaaa tttttgtgaa atttcggctt atttttcgaa gaatctactc ctggaacacc
    10801 gtgtatcaga ggttgtgaaa gagacgttca gccttgagcg atgataaaaa ctctcacaaa
    10861 cattgcgacg agtaacccac aggagcaata agaagcgatt ttgaaaaata gcttcccata
    10921 tttcacaaaa ttatgcctta ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa
    10981 aaagaactgt gacaagactt ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa
    11041 ctatccataa atacaaatac tagggttatt ctaacttctt ttattgcttg aatcagtcca
    11101 caagaaaaac agcctcttga ttcacaaata tttttttcga tattctcccg gcgtaatcga
    11161 ttccttcttt ttaaagaaac gaataaaatt ttgcggattt cgatattgca atctttctgc
    11221 aatctctttt acggagaggt tagtctcttt caaccatttt tttgctacct ctaaacgatg
    11281 attttgaaca aaatcaccaa atttttctcc tgtttccttt ttaaatacat tacttaaata
    11341 gttagggtta taatgcaacc tatcagcgat cacatctaaa gacagatcac gatcatattc
    11401 tgtgtagacg atatgcataa tattctccga caaattttta aattcatgat cagtgattgc
    11461 ttgagagcat tctgtgattg gcagaactaa ttgttctaaa acaagttttt ctagttcttt
    11521 gggatgataa ttgttcagtg cttttacata aatttgtttc atgttatcaa agttttttga
    11581 atctactccc aataactgtc ctaattgaac gagttcattg atcaagcgga ccgaaactac
    11641 ttcccgactc aaaggatttt tattttgtgc aaacaatgca tcaatcaatt catgtactaa
    11701 attcttcact tcctgatttc cagaacgaat ggcttcaaac aatcgatttt gttcttctat
    11761 cggatatcta ataagtgttt tttcatgagc agcaggaacg atttcctgat aaaaaataat
    11821 tgaacttgga ccagtattta cacgataata cagggcttct tttgcccgat caaccgattg
    11881 cttgctttca ttcagcgttt cgaaccggtt actgatgcct atactgatag ttaaattcaa
    11941 gtattctttt acacgttttt gaatgagtcg gcaatactcc atgacttttt tgcttgcttc
    12001 tgcatcatct ttatccattc gataaatagt ggcttgcatt tcttcattca aaacgatcgg
    12061 cagcattcga ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag
    12121 caaatcacgc tccccaccgt gactatcttc taacagatcg atttgaacca aagctgtata
    12181 atagcagtca ttttgagaac tataaccaaa ttgctgcaac cgctgatcta gttctcgcct
    12241 ttctactcga tttctaaata aactaagtac aaatagcgtt tccagctgtg gcttttgggt
    12301 aactaagtgt gcacttaacg cttctttttc tcctatgatc cgactgactg attgagcgac
    12361 caaatctagt tctttcccac tgaatccttt tgatttaaga ttcagacgtt cctgtatttg
    12421 agaaatcgga cgggcaaatc tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac
    12481 cagtgttaaa gacactgcaa ctagcccgat tctcagattg cggatcattg cgccaattgc
    12541 actagagtca acttctaatc tgtaaatcca attattataa tctgatttca gtactatata
    12601 cgttttatct ttttctctta tcactttcag ctgattgtaa tctagatcca cgtccgttaa
    12661 tacagttggt tgctctttat tttttactgt ctgagtagta aaaaggttgc caacttcgtt
    12721 ttcaatagaa agtatgtctt ctccactttc accaacaact tgttctattg tatgtttctc
    12781 tatcgtagca attcccaatg catatttttc tttttcaaac attggcaaag aaatcaccat
    12841 ttcgattcct ttgtttttct tttgccaaaa taaattattt ttgttattta tgtacttctg
    12901 ttgatacttt tcaacttcct cttcggtcag ttgactcaac gatccattga ttactcccca
    12961 tttaccagcc aaactgacta aatcataatt attatcatcc ataacaatcg tttctataaa
    13021 atttagttct ttcctgattt cagaataagt ggagtaatct gtatatgtca gcggatgctt
    13081 gaatatgtcc gagaaatttt tggtcgaacc atacgtagaa aacgaatatt caatcgttct
    13141 aattttccat tcaacgttag ttttaacttg cctgagcaaa ttctttttgt ctgttaaata
    13201 actcgattcg atcgattcat aggtattctg gtaaatgaat aaactaaatc ctatgacaag
    13261 tacggctcct aataaaaaca tcggtaaaaa tattttataa aatactgtac ttttcttcat
    13321 tttcttcctc tcctcctttt aaaataaaag gctttcattt ttctgtaaaa ttgatactca
    13381 tctaattaaa tgagcattcc ccgctttcaa gaatagcata attaaaaata aaaatccaag
    13441 taatttttaa cgtgtgcaat cttttcatat caaagatcta ttgacttatc gtagttatcg
    13501 taatatgatg aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg
    13561 atcaaagata tcgctaaagc agctggcgtt tctcatacga cagtttctcg tgctttgaat
    13621 aacagcagtt taataaaaaa tgaaacaaaa caaaaaatac gacaactagc taaggaaatg
    13681 aattattcgc caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga
    13741 ctatttttct caagtttgca taaaggaact tcatcaaatt tcctagcaga ttccattgca
    13801 ggcatccatt cagtattaga cacaaattac agtttatctg ttgaaagtat cgatgttgtt
    13861 gatttaacag aaatcaacct gcaacgctac gatggactga tcgttatgag tcaaagtgat
    13921 gatgatcagc ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat
    13981 cgtcacatca atgatccagc ggttatcaac gtcactgccg atgatgcggc cggtgttatg
    14041 caggcagtag attacggtat tggatgcgga catacgaaga tcgcttatat tggcggaaag
    14101 aaaaattttc gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg
    14161 agcatccctg tcaatccagc ctatttttta acaggagatt acagcttaga aagcggcctc
    14221 caaaacatgg aatacctatt atcactttct gacctgccta ctctggtttt ttgcggcaac
    14281 gacgacatag cgattggtgc attgagagct gtacatttag ctggcctccg tgttccccag
    14341 aacatttctc tgatcgggtt cgatgattct ccagtcgtct cctatctcaa tccgccgctt
    14401 accaccgtac gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct tttactagat
    14461 ttgatcgaag gcaggacgat cgaacaatct aaaattgagc tctccacctt gttgatcctt
    14521 cgtgagtcga tcaattactt gtaaactaca agtaatttta tataaaaatg ttaacgtgtg
    14581 cataacacag aaaggaagtt tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg
    14641 aagcacctgt aaaagttctt cagtttggtg aaggcaattt tatgcgtgga tttattgatt
    14701 ggcagattca gcagctgaat aaccaacaat tgtttaaagg taatgttgca atcattcaac
    14761 ctttagaaca aggtcttgga aatatgatga accagcaaga taatttatat acggtcattt
    14821 tacaaggact cagcaatggc gaaatcgttg atacttccga gattatcacc gtagtagaac
    14881 aaatgatcaa tccttatgat gaatgggatg cttatctatc tctagcagaa aatgacgact
    14941 tggcctatat cgtctccaat acaacagagg caggaatcca attcgtggag actgataatt
    15001 taacagatac tcccccacag agcttcccgg gcaaactgac agccttttta taccgacgtt
    15061 tcaaattaaa caaagctggc tttaccatca ttccttgcga actgattgac agaaacggcg
    15121 aacgtctaaa agaaatcatt ttacgctacg cagaaaaatg ggatttagaa cctgcttttg
    15181 ttgagtggtt agaaaaagaa aatgttttct gttgtagttt agttgatcgc atcgtcccag
    15241 gttatcctcg cgaagatgca ggtgttttca atcaacggca taactatgaa gatcaattga
    15301 tggtcaaagc agaaccattc atgctttggg tgatcgaagg gcctaaagaa ttggagaatc
    15361 aactaccatt caaacaggct ggcttgaatg tcatcatcac agatgatatg actccttatc
    15421 gtgaacgtaa ggtgcatttg cttaatggac cgcatacagc gatggtccct cttgctttgt
    15481 tggcggaatt ggaaactgta gaagaagtaa tgaaagatcc tttattctcc gcatatgttg
    15541 atcagttatt caatctagaa ttgattccta tgctttcttt acctaaagat gaattagcca
    15601 tttatgctga ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac aaattagaag
    15661 cgatttcatt aaatagtgtt tcaaagttca gcacccgtct gcttccagtg ttcaagaagt
    15721 atatcgaaga acaaaatcag gtaccaccgt taatcactgt ttcactagct gcgctacttc
    15781 ttatgtatcg aggcgatcaa gtaaagccac atgatgatga aaagacgata agcgaattta
    15841 ctgatgcttg gtctgataat ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt
    15901 ggggagaaga tttgtctcaa ataccgaatg tgacagatac agttcaagaa atagctgcaa
    15961 agatcgatca aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg atggtccatg
    16021 cttgaaaccc tgcaaccaac aatgaaatta aatcgacgag acagtgtggt cgtcgcatta
    16081 acccctattc caaaacaaac acaactgacg attgatcagc aaacgatcac cactcttgaa
    16141 gatatcccgc aaggtcataa gattgcattg ggtgatttga aagctggaga caacgtgatc
    16201 aaatatggct atccaatcgg tcatgtgacg acagacgtaa ctgccggtca atggctgcac
    16261 actcataatg taaaaaccaa tctttctggt gaattggatt atcgatatga aaaagatgtc
    16321 catccaagtc attacctttt tgagaaccga acatttcaag gttacttgcg aaaaaatgga
    16381 aaagtaggga ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt gaatggcatt
    16441 gctgaactga tcgtcaaaca gttcaaggaa aaacatccag atctgggaca gtttgaccat
    16501 attacgattt taaagcatcc ttatggctgt tcgcagcttg gaaaagatca tcaaaatact
    16561 cgagaaattt tagcggacgc agtaaatcat cccaatgccg gaggagtttt agtatttgga
    16621 ttaggttgtg agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa
    16681 gaacgagtga aattaatagt ggctcaagat gtttacgatg agatcgaaca aggtgtggct
    16741 ctattagaag agcttttaac agcggcggaa aatgaccagc gcatatcagt cccgctctct
    16801 aagttgaatg tcggtttgaa atgcggcggt tctgatggtc tatcgggaat aactgctaat
    16861 cctttacttg gtgcattttc tgactacttg attgctcaag gaggcagcac gatcttaaca
    16921 gaagtgccag aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa
    16981 gtttttgact caatcgttga cttgatcaat gacttcaaac agtatttctt gtcttacggg
    17041 gaaccagttt acgaaaaccc atctcctggt aataaagcag gtgggatcac tacattagaa
    17101 gataagtctt taggctgcac gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa
    17161 tatggcgaaa aaatcagaaa accagggctt agtttattgc aagctcccgg gaatgatctt
    17221 gtagctgcct ctgctcttgc ttcatccgat tgccagttgg tcttgtttac cacaggaaga
    17281 gggactccgt ttggctcata tgtcccaacg gtcaaggtat ctacgaatac aactcttttt
    17341 gaacgcaaag gtcattggat ggattttaat gcaggcgtct tattagaaca accaatggaa
    17401 gtaatcttag aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa
    17461 aatgaacaaa atgacgttcg agaaattgcc atttttaaaa acggagtaac actttgattt
    17521 tcataaaaca gtataaaaag aaagaaggaa gatttatgtt tttatctgat gattttttat
    17581 taaaagacga ttgggcgaaa aagctttacc attcctacgc aaaaaacatg cccattattg
    17641 attatcactg tcacttagat ccaaaagaaa tttacgaaaa caacaatttc agcaatctga
    17701 cagaagcttg gttaggaggc gatcattaca aatggcgctt gatgcgagct tgcggcgtgc
    17761 ctgaagaaaa aatcacagga aatgcttctg attttgaaaa atttttagct tggtgtcaaa
    17821 ccgtaccaaa aattatcgga aatccgcttt acagttggtc acatcttgaa ttgaagcgtt
    17881 tcttcggtat tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa
    17941 atgaaatttt ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag
    18001 aagtgatttg tacaacagat gatcccgcag acgacttgca ctatcatcag ttgcttgctc
    18061 atgaagaacc agatttgaag gttctacctt cattccgtcc agataaagca ctcaatattg
    18121 aaaaagaagg atttaatgca tgggtcacac tactggaaga agcagctgac caaacgatca
    18181 ccacctatca agaactgata gaagtcttag ggcagcggat agaatacttc catcaacatg
    18241 gatgtcgtgt ttccgaccac gctttagaca tcttacgcta taaagaagcg gatgaaacag
    18301 tattagaaga gatttttcaa aaagcacgag ctaacaaact attatcttca gatgaaatcg
    18361 atgcttatcg tacagaaaca ctgatacatt tgacccactt ctatcattct cataattgga
    18421 cgatgcagct gcacatccat gcttatcgta actgtaatac acaaatgttg gaaaaattag
    18481 ggcccgatac cggctatgat ggaatgaatg atctttcttt gactattcct ctgcaaaaat
    18541 tattgaaccg tgcagaagaa acggatcagt tgccaaagac gatcctttat tcattgaatc
    18601 cgaatgacta tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa cagaatggaa
    18661 aactgcagtt aggttctgga tggtggtaca acgatactcg agctggtatg cgcgatcaat
    18721 tgacacaatt tgctgatggt agtgctttgg ggaattttgt cggcatgctg acagattcaa
    18781 gaagtttcct gtcctacact agacatgaat atttccgtcg tgttctttgt gaatttttag
    18841 gcgaaatggt catgcgagga gaagcacctg aagatgaaca acttttagga agtctagttc
    18901 aagcgattag ttatacaaat gctaaaaatt actttggctt ttttgcctaa ttgattaata
    18961 gtaacagtaa cgaatcaaaa acaggatagt gaaataacgg aatccgttta tccacgtttt
    19021 agagaagaag acggaccaaa tttgctccgt cttcttctct tgctgttctg cttcattgcc
    19081 tataaacctt tatgaaagaa gagataacat gcctactatc catattgccg gtgattctac
    19141 tgcggcagaa aaagatgaaa acaagcgtcc cgagacaggt tggggagaaa aaatctctgc
    19201 ttatttttca cccgagatcc agatcaataa ccaagcaaag aatggacgca gcaccaaatc
    19261 atttatccag gaaggaaggt tagcccgtct agcttcacag tttcagccga atgatttctt
    19321 atttgtacaa tttggacata acgaccagaa aatccaagaa gaaagaggta cgcttccctt
    19381 cggtagctat ttaaaaaatc tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca
    19441 aatggtctta ctgacatctg tgacgcgacg agattactta gaaaacggaa cattgaatcc
    19501 agatatactt ggagactatc caaaagcgat gcgtgcattt gctgagaaac accatattcc
    19561 tcttttagat gtgttctcaa gatcacaaga attgttccag accttctcaa aagaagagac
    19621 aaaaaaattc tacctgcatc ttatacctga tacttgtaaa aattacccag aaggactaaa
    19681 agacaatacc cactttagtc cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt
    19741 gataaaagaa tcccgtctgc ctcttgctaa ttatttacaa aaaggagttt gagatgtttc
    19801 cattaaattt aggcatccgt gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc
    19861 aaaaagtatc acagcttcac ttgtcccata tccaatttgc accacaaaaa gcatttcctg
    19921 aacaacttaa attaactgac atgacacttg ggagtgctgc ctattttggt gaatacttcc
    19981 aaaaaaacaa tatcgaactt tccattttag gatgctatat caacctatcc agcaaaaatc
    20041 aagaagttcg tgcacaagca ttggcaactt tcaaacagca aattgctctg tgctctgcct
    20101 atcaagccaa gatgattgct acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa
    20161 attttactga agaagcttat cagatcgcca gaaattctat cattgaatta gtcgctcatg
    20221 cagaaaattt tggcgtaact gttgctgttg aagcagggat caatcatcca atctataact
    20281 atcagttagc taaacgttta atcgatgaag tagcttcacc aaatttaaaa atcattttag
    20341 attgtgcgaa tttgatgcat aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg
    20401 ccttggacaa tttacatggt tatattacag cgcttcatct caaagactat atcatggaaa
    20461 atggaaagat ccgcatggta cctgttggca agggctgttt agatttccgc ccggtactgc
    20521 attatatcaa atatgagcgt cctctaatgt acgccactct tgaagctact cctgagattt
    20581 atgttcctga agcaatagaa cacttgcagt ttatttacca agattgttaa ggagaagatt
    20641 atggaggaac gaatacagaa aagttatcaa aaatatcatg caaatgcttt tcagacattg
    20701 ctgcatcttt atcagggaaa ttattggcgt tttgccatgt caggactctt ttttattatc
    20761 aaacatcttc catcttggtt aatgccgatc gctattgcca atgtaatcaa tgcagcttca
    20821 tcgaaagatt cttctcagtt gcaggccatt tatttcaacg cttttttgat ggcttgtctg
    20881 attggtcaaa atatattgac gaattatttt catgtaaaat accatagcac ttcgatccgt
    20941 caagtagaag ctggactaag agcgaccctt atcaagaaaa tccaagaact atctattcct
    21001 tatcaaaaag aattgcaatc tggacgaatc cagtcaaaga tcatccgtga tgtagaagct
    21061 atccaaacgt tgtcctctca agtatttgtc agcggattaa atatcgctgt caatttattc
    21121 gtagctttag gtatcacgct ttataaaagt cctgttgttt tcatgttttt cttattgact
    21181 gttcctctcg ccgcattcgt tactctcttt ttccgtaaaa ggatcagaca gtccaactca
    21241 gacttcaggc aagaaatgga agagacttct gcaaaagtga tcgaagtgat cgaattagca
    21301 ccgattgctc gagctcatgg gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt
    21361 actcaaatct atgaaaaagg attccactta gatatcctgc aatctgtttt cggatcagtc
    21421 agctgggcta gttttcagat atttcagctg atttgtttga ttttcactgg gatattagct
    21481 ttacaagggc gcatccaacc cggcgatgtc gtgatgtatc agacgtattt cacgacgatc
    21541 gtcaattccg tttctggaat gatcacgctg attcctacga tttcaaaagg catggaatct
    21601 atcacttcta ttggcgaact gttgattgct ggcgatgtag aaacttatca cggtaagaaa
    21661 aaaatccaac atttggatgg aaattttcgt tttgaggatg tttcttttct ttatccggac
    21721 tcactcaaac cgattatcga tcatttttct ctagaggtga aagctggaga aacaattgct
    21781 tttgttgggc catctggctc tggaaaatct acactcctta acttattgat tggatttatc
    21841 cagccgactt caggaaaaat ttttctagat gatcagcctt tggatgaact ggatatgcga
    21901 agtgtgcgaa attatttagc cgttgttcct caaaccactt tgttgttctc cgcttcgatc
    21961 aaagagaaca tcacttatgg cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc
    22021 gaagctgcgc aactttcttc actgattgac cagcttcctg atggtctgga taccttagta
    22081 ggcgaacatg gcaacaagct gtctggtggc caaaagcaac gtatctccat tgcacgagcg
    22141 ttgatacgtc agccaaaaat cattttgcta gatgaagcaa cctcagcttt agataaccag
    22201 tcagagaaaa aaatccagca agccctgaat tacctgacag aaacacccac tacttttatt
    22261 gtggctcatc ggctttcaac gatcaaagag gcagataaaa tcgttgtgat cgatgaagga
    22321 aaaatcgttg aaataggtac ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc
    22381 ttgaaaaatg aatgacaaaa aataggagct atgacaagac ttttgtcgta gctcctattt
    22441 ctgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca
    22501 tgagaagctg ttttttgaaa ttgttccttg taactcagag ttaaacggta ggctcacgac
    22561 ctcttggtaa atggtgttca agagacagcc tatcggcaat aagaagcaat tttgtcatcg
    22621 ctcaaggccg aacgtctctt tcacaccctc tgttgtttca taaaatagtt ttattcgctg
    22681 taaaggtcgc gaactggtgt catgatgata cggttcaagt cattgatgat tgtactgaag
    22741 ccttgctctt tttgcatcaa gtcattgatt acttcttctg attggatttt cacagccatt
    22801 tcttgtgctt tcattgcatc ttcgtcgctg aactcttgtc cactcatttg tttttcttgt
    22861 aattcgattt gcatcgcttg gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt
    22921 actttctcgt acgcttcttt caacgctttg aattctggta attcacggat ctcacgttcg
    22981 atttgattcg cgctatcgta gatgtttgac atgttatgcc ctcttttctt ttagtagtta
    23041 ctctctattg taacatgggg ttattcgttt tcaaaggggc tactctcctt gatatttccg
    23101 ccagaggtct ttagccgctt caccaattcc ctgaacgcct tctttcacca tatcgccgaa
    23161 gtttttcagc ccttcttttg cttgttcacc atattgattc aagttatctt gccaatttcc
    23221 agactctcca ttagaatttc ctgtgtttcc aacttgatct gcagctacca cttgtcctcc
    23281 tgtggcatag gcatctgcta cactaaatcc tgtttttgaa gtatacggaa ggatgccttc
    23341 tgccgctgat ttgaagactt tcccgacaac atttccactt gtcccttcca aatagtgcag
    23401 cttgctggat tcttcgtaac ccatccatgt cgaaatcact acgtctggcg tgtaaccgat
    23461 catccattgg tcatttgctt ttgtggcatc gaaattagtt tctgtggttc ctgttttccc
    23521 tgctatcgta taacctgcag gattcgcagc agccgctgtt ccatttgaaa atgtaccaag
    23581 aagcatgctg gtcatttcat cggctgtttc ctttgatatg acctgttttg gttttttgtt
    23641 tgtgttatcg acgatcaccg ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt
    23701 atacatctga ccatcattgg caaatgcact gtaagcaccc gccatgatca atggagatac
    23761 tcctttttcc agtccaccaa gggctaagcc ataatactta tcagattttg ctaaaggcag
    23821 gccgaattct tctgctttat catatccttt ttgtaaaccg atctcatgca aaagccatac
    23881 agtaggcaaa ttcaaacttt gtgcaacagc ttgatacatc ggtacttctc cttggtatgt
    23941 cccgtcaaaa ttatgcgcat cgtaataact ttgcggttcg tctttcaaga tactggaagg
    24001 cttgtatcca gcttccagtg ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg
    24061 tgaacgacgc atctgtgtag caaagctgaa tcctctaaag acatgatctc ctcttcgtcc
    24121 tactagtgct tgtacaccac ctgtattcgg atctaaagct actgaagcag attggaccat
    24181 ggcaccatct gaagcatttg ctgggaataa tgcactgttt tgataagtag cttctaattg
    24241 cttttgataa tcctgatcta aagctgtata gattttatag cctttattca tgacatcttc
    24301 ttcatcaaga ccatactcgt taactgcttc atcgatcact gcatcaaaat aataaggata
    24361 acgataacca gaatctgaat tatcgtatgt gtcattaaga tacgcgctga tatctgtctg
    24421 gctttctgct tgttcttgtt cttttgtgat ttttccgttt tcttccatta cacttagtac
    24481 tgtgtttttc cgatcattcg cattttttgg gtgatcgatc ggattataaa taccaggacc
    24541 tttcagcatt cctgccaaag tagcagcctc agacagcgta acattcgttg catctactcc
    24601 aaaatacttt ctggaagcgt cttggacacc ccataccccg ttgccaaaat aggcattgtt
    24661 aagatacatg gtcaagattt cttctttgct gtacttcttt tcgatctcga ttgccaaaaa
    24721 taattccttc gcttttcgat caaacgtttg gtctaatgtc agatacgcat ttttggcaag
    24781 ctgctgagtg atcgtacttc ctccgccccc tgatgttccg ccagacaaga ccattcggac
    24841 tgctgcacgg gcaatccctt tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc
    24901 tgtcgagatt actgcatctt ggatataggg agaaatcgcg tttaattcta catatgttcc
    24961 tttttgggcg tacagtgtgc cagcttcttc gtttttctta tcataaatga ctgtcgattg
    25021 gcttaatcct gattttaacg tttcgacatt tgcttgtttt gctaaaataa agaggtagat
    25081 acttgtcact aagacacaga tcaaaccaat cagcaaaatc aatttgttca catgatattt
    25141 tttccagatg cgctttcgcc atcgatggaa ttgtccgaga tatggtctga tccaacgcca
    25201 aaaactgatc aaagcagctt ttatcttttc taaaatgctt tgaaaatcca tttgcttctt
    25261 ctcctgtcta tttgattcaa tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg
    25321 cttaagttgt agttcaatat gtttttttac gttagtgcaa gtagagtaac ataagaatga
    25381 acagaaaaat gtaagttttt tcttaaatat gttttaaaaa tggtacaatt agtctgcgat
    25441 tgcactgaaa ggagtacact tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa
    25501 acaacaattc gcgacttgct tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt
    25561 ttgcgtatac gtaaaaacgt ttggatcaat caacaacctg ctttgttcca tcatgaagta
    25621 aagggcaatg accagatcac attgttattc gaagaaacag attataatta tcaagaagtt
    25681 caattaggag atgcgaagca catccgtgtt ttatatgaag atgagcattt gattatagtg
    25741 gataagcctg caggaatgaa gactcatcca aatgaaccga cagaaaacag taccttactc
    25801 aatcatttag ctgcttatct cgacactaaa cagcaacgac cttacgttgt ccaccgcttg
    25861 gacaaagaaa caagcggtgc cgttttattt gcaaaaaatc catttgtctt gccgatactt
    25921 ggaagaatgt tggaacaaaa aatgatctat cggagatatc aagccatcgt atggggagaa
    25981 attgccaaag atatgaccat caccaataaa attggacgag atcgccatga tcggagaaaa
    26041 agggtcgtag atgataaaaa tggtcagagc gccttgacac atgtcactgt caacaaagta
    26101 ctttctgaac aaacacaagt ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga
    26161 gttcatttag ctcataaggg acatccgatc gttggagacc ctctctacca aaatagaaca
    26221 gctgaacgac tgatgctcca tgcattagaa ctgcacatga tccatccatt tactcaagaa
    26281 aaaatagtcg gtaaagcttt acctggatta tgggaataga aataaaaaag aacagagtct
    26341 tatcagcaga taatgataag actctgttct ttttgtgtaa tttgtctgct tacttttgat
    26401 gttcgtgttc tgctaacagc aaactaccga taatcccact ttcattcggc agtccccaac
    26461 gaacgatata ttcacttact tgaggtgttt ccatataacc acccatcaat tcaacaaatt
    26521 gctgacggat tttttgaagg agatgatcct gattcattac cccaccgcct aaaataatct
    26581 tttcaggtgc taacgtcaac gtatagttga tcagtgcttg agcaatataa taagcctgaa
    26641 tagcccaaac aggatgatct tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa
    26701 tagacggtcc tgctgctaat ccttctaaac aatctttatg ataaggacaa gtaccttcat
    26761 aatggtcatc tggatggcgt ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg
    26821 aaaaaatttc ttcattgatc acgacacctg caccgattcc tgttcccacg gtaaggtaaa
    26881 tacaactatt ttttccttgt gctgctcctt gcttcaattc cccatatgca gcagcattga
    26941 catccgttgt ccaagcagca ggaatattgt agcgttcctt gattgtgcca agaaaatcga
    27001 attgtgccca cccttttttg ggagttgcca aaacgtatcc gtaattatct tttgttttgt
    27061 cgataccaat cgggccgaat gagccaatcc cgattccctt caattcataa cgatcaaaaa
    27121 attcaaatac ctgatccaat gtttcttctg gtgttgtggt agggatactt aatttttcta
    27181 aaatttctcc accatctgaa accgcacaca caaattttgt tccgcctgct tctattccgc
    27241 catacattcg ttttcctcct cgaatccgat tgtccgaatc tcataaggtt ggattattgg
    27301 gacaaccgct tgcttcgttg gttcttcaag cagattcaat atgacagttt ttccattgtc
    27361 tttagttatt tccaattttt ctaaatgact agacatattg aaacctctta ctactacttt
    27421 atcactaaac ttgctgcgtt tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa
    27481 atattgttgt tttgaaatca atgttccttc atgatgtttg atttgttgag tggaaaaagg
    27541 aatctgtgct gcgtatgcat gcaaataagt agagaatttt tcttctggtc catgtagttc
    27601 aatgctgtaa ttgaaccgat gctcacctag acactgtgct tctggtgttg gaaaatatcc
    27661 ccaatcgcct agttctccca cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt
    27721 tacttcatat tcattcaaac caaagtttcc gactgttaca ccgtactctt catcatgaat
    27781 attgacaaat gcttgctgat gttgcggatt cgtggggttt tcccaacttt tagacacatg
    27841 gtttgggcgc ttcaccactt caaaaatact atctgcttcg tgatgttcca catgtaactt
    27901 tgtaggaaac aagacacgta agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa
    27961 gtcgattttt ttactatctt ttctgattgt catgattgtt tttatttgaa ggattcgttt
    28021 ttctttggat cgttctgctt ttcgataacg aaattccaca accatctgct gttctttttc
    28081 taaacgctcg tctgcagaaa cagggatttc catttcctgt agaatactta tttttgctat
    28141 ttccggtgta tctactaata cttttacttc agagttttct ttgttactgg ataggattgg
    28201 ctgatcacag aaaggttgtt taaagatata ctcatttcct atatcgccga tatcttcaaa
    28261 aatatgcaga ttattcaact ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt
    28321 tttttcaatc gtcaaatgta gagggccgtt ttcgatgatt cgtccactct gatgaatcat
    28381 tgtttccttt tcttctgctt tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa
    28441 tgctggcatg ttttctaatg gtaaagtcac tttgatcatt cttgccatat atggcacacg
    28501 gaaacggtct ttcggcaaat cataaccaaa ttggacagtt tcttcagata aaatagctgg
    28561 tatagtggcc ccactttttg tttctacatg atacttacgt ttaggcaaat tttctaactc
    28621 ttgatatagt tgttcaggta ttccttcagc gaatcttttt ctttctaatt cgatggttat
    28681 ttctgcttca ccagttttat ccattcctgc agtatttaca atgacaaatg gaaagctgtc
    28741 ttttgggaat ccggtcgtat cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag
    28801 atactttcca acttcatctg ctttttcaaa acgaggtatc atttcccgat ggacagaatc
    28861 gactgaacaa ccgcaaatac tgtcgtgagg atgattttgc attaatgtct tccaagcata
    28921 atctaattga tcatggggat agtttccact aacttcataa gccatcgtcg caagtggttc
    28981 tgtaatattt tctagctgtc gctggacctt ggtattccat tgttttaagt aaacccgagc
    29041 agatgctgta ttggctaaag tataccagcc atccgtttct tgacttgtca attctccttc
    29101 tactgagcct aagtcttctg gcacatcttt ttgaactgct tccagatagt cagtaaaatt
    29161 agaatgaata aactcgtaat ctggaaacag ttcatttgcg agatggattg cttttgatat
    29221 atctttttgt accggttgat gatcaacacc attcatcatc aataagtggt tcgtagaggc
    29281 atattgttct gcatccgcta atttttgctt ccaaaaggca agagctgctt ctttttctac
    29341 tggaatctca ttcccgttgc tataccaatt ggcaaatagc agaccaaaga ttgctgtttg
    29401 atcagggcct ttccaccaca tctctgagta ttgggaagaa tagttttctg cttccaaaac
    29461 ttgattatca aagccaattg gttttacacc acggccaaac gctgctgctg aaataccagc
    29521 ttgtttcatt aattgaggcg tctgtcccat atttccaaat gtatcgggaa aatagcctaa
    29581 catgacaggg gttccccatt tacggctttc ttccatgcca atcagcatat ttctcacgtt
    29641 cgattccgag ctgataagaa aatcatcttg caagatataa aatggtccaa ttctcaattt
    29701 tccttcattg attgcttgtt ggattgcctg tcttttttct ggccttactt gaagatagtc
    29761 atctaaaata atcgtttgcc catccaaatg aaaactgttg aaggacggat caacttcaaa
    29821 tagctctagt agatcgtcta tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat
    29881 gtaccattca cgatcccagt ggctatgaga aatgatatat actttctttt tcatttacga
    29941 tacctcctac ttttctactc taatatcaaa gtaatccatg actaattcgc aaaacatcat
    30001 atttgcccaa gaaaaccatt ctctcgtata attttcagga ttgttcacat cgaatccttc
    30061 gtgcataagg tgtgttcctg cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc
    30121 agatttgtct gacgttgtca ttccttccat agctaatgca atcggccaaa cgtatttttc
    30181 tggcgtatgg gaggatccaa ttccttttgc gtactttcct tcatagaaat atggattttc
    30241 tttactcaac aatgttcttc ttgtttgcag ataatcttcc tcttgttcag aacaatatcc
    30301 caagtaaggt gcggcgatca gattgggcac attactgtca tccatgactg tggcgtttcc
    30361 cattccatct acttcatatg cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg
    30421 attctgaatc ccctcttgga tctctttctt caatttctgt gtttgctgta caatttcttt
    30481 atcatcgagt atcgtttgaa aaatttcttc cacataacct aacacgacca ccgcaaacat
    30541 attggatggt accagatatc cataacggca agcatcatcg ctaggacgga aaccagacca
    30601 tgtcattcct gtaaaagctg tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt
    30661 tcttgtcgtg tctcgtacaa atgtataagg agagtgttca tgatcttgct ctgttttgaa
    30721 cacgtccaag atttttttga taccttcaat aaaagattga ttgaattgat cagtttttcc
    30781 tgtatttttg taaagaagat aggccaactg tattgggtag cataaagaat caatttcata
    30841 ttttctttcc cagatccaat cgttcatttt tgtatgatct gtttggtggc cagcaccgtt
    30901 agcttcttcg ttgaaggcat tcgcatatgg atcgatattg atataaaaaa attgcttttt
    30961 gactaacccg ctgatcatgt cagctaattc ctcatcttct tttgcaatca ctaaataagg
    31021 acgaacttgt gcagtagagt ctcttaacca catcgctgga atgtctcccg tcaataaaaa
    31081 ggtggttccg tcattttgct ttttgactgt tgtcaacaga gtattggcaa atgctgctgt
    31141 aaaattcttt gcccagtccg catggatatc cccacatttt tgtgtgatat cttccataaa
    31201 tttctgcacc gattttggta tatctttata aaccactaat aaaaccttct ttctatttga
    31261 tatatcttta aagattatac ttcatacagc caattatgtc tacgctttca aaaaatatct
    31321 ttttatttac tttttcatcc attatggtat tatttgaata tgatatatca ttaagaggtg
    31381 tttatatgac tcaaccattg taccagacaa tatttaatga tttgcaaacg gctattttaa
    31441 ctggtgaatt acctgtagac agccaactac caactgaaaa agaactttca cgtacctata
    31501 aagtaagcag aatcacttcc aaacgtgcat tgactgaatt agagcaatta ggacttattt
    31561 atcgcgttcg cgggaaaggc agtttcgtta aatccgcttc tccggaacag attcttgctt
    31621 ctcctacaaa aacacgacgg attctgtttc ttcttccata tgtcgctgat ttgtctgttg
    31681 gtgattttag caagggattg aaccctgtga tgcaagataa acagattgat gtcatgatga
    31741 cgacgcttga ctttctacaa aataaaacag caaaagagct aatcaatgaa tttgacggat
    31801 tgatttatta tgctcttgat acagaacaac atcttgactt actttttgag ctttcattga
    31861 aagaattccc tgtcgtcata ttagataaaa aaatctatga gttacctttt cctaccgttc
    31921 tttccgataa tttccaaggc ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt
    31981 caaaaatcgc ttatttgttt ggtacgcaga ctcatccaca atccgttcga cagcgttacc
    32041 ttggttacct tgaagcattg gacaaagctg gtttgacttt ccacacttca ctggatgaca
    32101 aaagcgcgac attagatgaa ttgctttctt atatcactaa gtatgaagta actgcttttg
    32161 tttgtgaaaa cgatgttgtc gctatccaga ccatgaatct cttgagacag catggttatc
    32221 atatccctga agatttttca attatcggat ttgatgatat ccaagcagca gctcttgttg
    32281 acccgccact gacaaccatc gcacaagatt ttgaacagtt aggtaagctt gcgggagatg
    32341 ctttggtcac ttggttagag acaaaagatc gaccatccga tagtaaattt ccagtttcat
    32401 tcatcaaaag acaatcaaca aaggagaaat aaaatgaata tcggtcaaat agatactaga
    32461 catgcaacag cgaatcagcc aaatttctct aatgggaact gccagcctta tactggcgtt
    32521 ccttttggga tgaattattt tgcaccgcaa actacggatc aaaacggcag ctggtggttt
    32581 catcctgatg accgcgtttt tcaaggctac cgtcttaccc atcaaccaag tccttggatg
    32641 ggtgatttca gtcatatgct gtttacgccg atcaatggaa aactacagga gaacaccctt
    32701 ttccatgctc aaagttctta ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg
    32761 atctgtcaat tacgttatgg catctgttct acattgatac ctagcatgta tggcggaatc
    32821 ttatctattg attacagcaa aaatgacagc ggtttacttt tatcctttcc gggacgccat
    32881 cagctctttg ttatagatcc ttacacagtg gaaggaaaaa tcacacattt tgctggttca
    32941 gaagatcctg atttttcttt ttattttata ttgagatttg aatatccttt aacaattgat
    33001 gagttagctg tttccaacaa aaatagcgac tcgatcccgt tgtattttga acagacaaaa
    33061 aaacaaacta tccgatttgg gacatctttt atcagctcgg aacaagcagc ttataacctg
    33121 gaactagaac aaaaccaaag caaagagact taccttgctc acagtcaaga acaatggcag
    33181 tcctattttg atcgtataca gctcacacac catgatcaac aacaattgcg gacgttttat
    33241 cataatttat atcgggtgtt tttatttcca caaacatttt atgaaattga tcctagtggg
    33301 caaaaaatcc actatgatac gactagtcga tctataaaac ctggtgttct ctatacaaat
    33361 aatgggtttt gggatacgta taaaacggtt tatcctcttt attctttgat tgctgtagaa
    33421 aaatacgaag aaatgctgga aggttttttg aacagctata gagaaagtgg ctatttgcca
    33481 aaatggctgt ctcctgatga acgtggactt atgcctggga cattgatcga tgcggtcatt
    33541 gcagatgctg ctgtaaaaga tatccgacga gacttgatgc ctgaatttct aacggcaatg
    33601 gaaaaaggtg ctacggttca aagcaaacat ccaaattatg gacgccaagg aacagaagat
    33661 tacttgacat atggctatgt tcctaatcac taccacgaat cagtcaatca cacattagac
    33721 tatgcttata gcgatttttg tattagccaa gttgccaagg tactagaaga agaggaaatg
    33781 acgaagaaat atcagcagca atcattgaac tatcaaaata tctttgacta tgagtcagga
    33841 tttatgcgtt caaaagataa agatggcaag ttcagagaac cattttcagc taatcgttgg
    33901 ggacaagatt atgcagaagg aagtgcttgg caaagcagct ttgctgttta tcacgatttt
    33961 tcaggactga tcggaaaata tggtggcagt gagagatata aagaaaaact cattgagctg
    34021 tgcaatcagc ccccaacgtt tgacccagga ggatatggtt ttgagatcca cgaaatgagt
    34081 gagatggccg cgatagaatt tggacaatta gctatttcaa accagccaag ttttcactat
    34141 ccttacttgt tcagttatat aggacaacct gaaatggcac agccactgct taaacagttg
    34201 atgactcaga ccttcgacga ttctccaatg ggttaccctg gagatgagga caatggaagt
    34261 atggccgctt ggtatatctt caatagtctg ggattttatc ctgtcacgcc tggaaccggt
    34321 gaatatgtta tcggtatgcc attaattgac aaggctgttt tgcttttgtc aaatggaaag
    34381 gaattgacga ttgaagcaac gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa
    34441 cggatggaca gagaatatac gaaattatat tttacccatc aagatttgct gaaaggcggc
    34501 acgatctctt accaattagg gatcatccct caccctaaga aatatcaagc aaaagatttg
    34561 cctttttcct tatcaaacta taattaacaa gaggttgtga aaaagctgtc ctgcatcaag
    34621 taataagaac agccaagcaa aaatagatcc tcatattttt cgtttggttg gtcttattac
    34681 cgcagatgca agcttttgaa caccgtttat tgagaggttg tgaaatcagc gttctgacct
    34741 gagtattaga gagacaaatg gcaaaatagt tcctcatatt ttgccatttt ttgagctaat
    34801 accgagggtc agctgattga acaccattta gtggagaggt cgtgaaaaag ctgtttagtt
    34861 tcgagtatta agaaaaattt tgtgaaaatg gctttc
//
LOCUS       DAYYAG010000014        34588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000014 DAYYAG010000000
VERSION     DAYYAG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.014"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHARQ_000618"
     CDS             212..604
                     /locus_tag="ACHARQ_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8409069.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHARQ_000619"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHARQ_000619"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8409070.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHARQ_000620"
     CDS             2698..2943
                     /locus_tag="ACHARQ_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409071.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHARQ_000621"
     CDS             3165..4775
                     /locus_tag="ACHARQ_000621"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8409072.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHARQ_000622"
     CDS             complement(4752..4964)
                     /locus_tag="ACHARQ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409073.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHARQ_000623"
     CDS             5067..5936
                     /locus_tag="ACHARQ_000623"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8409074.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHARQ_000624"
     CDS             6101..7372
                     /locus_tag="ACHARQ_000624"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8409075.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHARQ_000625"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHARQ_000625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8409076.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHARQ_000626"
     CDS             8763..9026
                     /locus_tag="ACHARQ_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8409077.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHARQ_000627"
     CDS             9471..9920
                     /locus_tag="ACHARQ_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8409078.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHARQ_000628"
     CDS             9977..11395
                     /locus_tag="ACHARQ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8409079.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHARQ_000629"
     CDS             11395..12255
                     /locus_tag="ACHARQ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8409080.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHARQ_000630"
     CDS             12429..13202
                     /locus_tag="ACHARQ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8409081.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHARQ_000631"
     CDS             13349..13714
                     /locus_tag="ACHARQ_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8409082.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHARQ_000632"
     CDS             complement(13862..15070)
                     /locus_tag="ACHARQ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8409083.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHARQ_000633"
     CDS             complement(15089..15367)
                     /locus_tag="ACHARQ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409084.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHARQ_000634"
     CDS             15560..16216
                     /locus_tag="ACHARQ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8409085.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHARQ_000635"
     CDS             complement(16658..17455)
                     /locus_tag="ACHARQ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8409086.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHARQ_000636"
     CDS             complement(17452..18636)
                     /locus_tag="ACHARQ_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8409087.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHARQ_000637"
     CDS             complement(18661..19908)
                     /locus_tag="ACHARQ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8409088.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHARQ_000638"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHARQ_000638"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8409089.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHARQ_000639"
     CDS             21888..22202
                     /locus_tag="ACHARQ_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8409090.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHARQ_000640"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHARQ_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8409091.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHARQ_000641"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHARQ_000641"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8409092.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHARQ_000642"
     CDS             23546..23875
                     /locus_tag="ACHARQ_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8409093.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHARQ_000643"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHARQ_000643"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8409094.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHARQ_000644"
     CDS             24863..25687
                     /locus_tag="ACHARQ_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8409095.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHARQ_000645"
     CDS             25680..26027
                     /locus_tag="ACHARQ_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8409096.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHARQ_000646"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHARQ_000646"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8409097.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHARQ_000647"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHARQ_000647"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8409098.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHARQ_000648"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHARQ_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8409099.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHARQ_000649"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHARQ_000649"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8409100.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHARQ_000650"
     CDS             31684..32451
                     /locus_tag="ACHARQ_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409101.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHARQ_000651"
     CDS             32441..34483
                     /locus_tag="ACHARQ_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409102.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11119 a         6130 c         7259 g        10080 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttat
//
LOCUS       DAYYAG010000015        34365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000015 DAYYAG010000000
VERSION     DAYYAG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.015"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(259..996)
                     /locus_tag="ACHARQ_000652"
     CDS             complement(259..996)
                     /locus_tag="ACHARQ_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8409103.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(998..2500)
                     /locus_tag="ACHARQ_000653"
     CDS             complement(998..2500)
                     /locus_tag="ACHARQ_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8409104.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHARQ_000654"
     CDS             2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHARQ_000654"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8409105.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHARQ_000655"
     CDS             4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHARQ_000655"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8409106.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            7842..9107
                     /locus_tag="ACHARQ_000656"
     CDS             7842..9107
                     /locus_tag="ACHARQ_000656"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8409107.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9111..9842
                     /locus_tag="ACHARQ_000657"
     CDS             9111..9842
                     /locus_tag="ACHARQ_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8409108.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            9977..10861
                     /gene="rapZ"
                     /locus_tag="ACHARQ_000658"
     CDS             9977..10861
                     /gene="rapZ"
                     /locus_tag="ACHARQ_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8409109.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            10858..11856
                     /gene="yvcK"
                     /locus_tag="ACHARQ_000659"
     CDS             10858..11856
                     /gene="yvcK"
                     /locus_tag="ACHARQ_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8409110.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            11890..12825
                     /gene="whiA"
                     /locus_tag="ACHARQ_000660"
     CDS             11890..12825
                     /gene="whiA"
                     /locus_tag="ACHARQ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8409111.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            13393..14049
                     /locus_tag="ACHARQ_000661"
     CDS             13393..14049
                     /locus_tag="ACHARQ_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409112.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            14228..14793
                     /locus_tag="ACHARQ_000662"
                     /pseudo
     CDS             14228..14793
                     /locus_tag="ACHARQ_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15492..16013)
                     /locus_tag="ACHARQ_000663"
     CDS             complement(15492..16013)
                     /locus_tag="ACHARQ_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8409113.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            16211..19891
                     /gene="nifJ"
                     /locus_tag="ACHARQ_000664"
     CDS             16211..19891
                     /gene="nifJ"
                     /locus_tag="ACHARQ_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8409114.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            20080..20967
                     /gene="cdaA"
                     /locus_tag="ACHARQ_000665"
     CDS             20080..20967
                     /gene="cdaA"
                     /locus_tag="ACHARQ_000665"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8409115.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            20964..22133
                     /locus_tag="ACHARQ_000666"
     CDS             20964..22133
                     /locus_tag="ACHARQ_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8409116.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            22157..23512
                     /gene="glmM"
                     /locus_tag="ACHARQ_000667"
     CDS             22157..23512
                     /gene="glmM"
                     /locus_tag="ACHARQ_000667"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8409117.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="ACHARQ_000668"
     CDS             complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="ACHARQ_000668"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8409118.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            25799..26089
                     /locus_tag="ACHARQ_000669"
     CDS             25799..26089
                     /locus_tag="ACHARQ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409119.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26102..27127
                     /gene="sppA"
                     /locus_tag="ACHARQ_000670"
     CDS             26102..27127
                     /gene="sppA"
                     /locus_tag="ACHARQ_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8409120.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            27130..27789
                     /locus_tag="ACHARQ_000671"
     CDS             27130..27789
                     /locus_tag="ACHARQ_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8409121.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            27946..28878
                     /locus_tag="ACHARQ_000672"
     CDS             27946..28878
                     /locus_tag="ACHARQ_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409122.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            29032..30321
                     /gene="tig"
                     /locus_tag="ACHARQ_000673"
     CDS             29032..30321
                     /gene="tig"
                     /locus_tag="ACHARQ_000673"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8409123.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            30568..31818
                     /gene="clpX"
                     /locus_tag="ACHARQ_000674"
     CDS             30568..31818
                     /gene="clpX"
                     /locus_tag="ACHARQ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8409124.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            31901..32488
                     /gene="yihA"
                     /locus_tag="ACHARQ_000675"
     CDS             31901..32488
                     /gene="yihA"
                     /locus_tag="ACHARQ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8409125.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(32562..32717)
                     /locus_tag="ACHARQ_000676"
     CDS             complement(32562..32717)
                     /locus_tag="ACHARQ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8409126.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            32853..34181
                     /locus_tag="ACHARQ_000677"
     CDS             32853..34181
                     /locus_tag="ACHARQ_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409127.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        11210 a         5943 c         7431 g         9781 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
       61 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
      121 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
      181 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
      241 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
      301 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
      361 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
      421 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
      481 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
      541 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
      601 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
      661 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
      721 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
      781 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
      841 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
      901 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
      961 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
     1021 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
     1081 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
     1141 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
     1201 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
     1261 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
     1321 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
     1381 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
     1441 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
     1501 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
     1561 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
     1621 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
     1681 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
     1741 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
     1801 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
     1861 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
     1921 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
     1981 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
     2041 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
     2101 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
     2161 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
     2221 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
     2281 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
     2341 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
     2401 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
     2461 ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc
     2521 aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa
     2581 tgaaaaaaat gcgagtttct aacattttat gttataaaac gtaacatgca taataagata
     2641 aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt
     2701 tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat
     2761 gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga
     2821 tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat
     2881 cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt
     2941 gaataaacca acactaatta ttgcccataa caaaacattg gctggtcagc tgtacgggga
     3001 attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta
     3061 tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa
     3121 tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt
     3181 gatcgtcatt gcatccgttt catgtatttt tggattaggt tcgccttttg agtaccaaaa
     3241 acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt
     3301 agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg
     3361 cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt
     3421 ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg
     3481 agaaacggaa cacgtggcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat
     3541 ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa
     3601 tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat
     3661 gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg
     3721 taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt
     3781 ggcggacgaa tcgcacgtaa ccatgcctca agtcagagga atgtataatg gtgaccgagc
     3841 gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc
     3901 acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc
     3961 aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg
     4021 gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg
     4081 cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa
     4141 aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca
     4201 tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt
     4261 tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct
     4321 tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca
     4381 aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt
     4441 aacagattct atgcgtctag cgatggatga aacatcaaga cgccgtacga ttcagcagaa
     4501 gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat
     4561 ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtatgaaga
     4621 aatgacgaaa gaagaaaaag atacgttatt gatgaaatta gaaaaagaaa tgaaaaatgc
     4681 ggcgaaagca ttggattttg aaacagccgc taatgtgcga gatatgattt tagaattaaa
     4741 agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcacg gtgcacgtgc
     4801 ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg
     4861 tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg
     4921 gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc
     4981 ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag
     5041 taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt
     5101 gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc
     5161 agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt
     5221 ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa
     5281 agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga
     5341 gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga
     5401 agggattcgt tctcgtctat ttgattcttt agaggcggct ctgcgattgg cagacggcta
     5461 tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc
     5521 ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt
     5581 tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt
     5641 gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag
     5701 ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga
     5761 tacaccattc gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga
     5821 tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt
     5881 cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg
     5941 tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt
     6001 gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt
     6061 gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt
     6121 aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg
     6181 actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg
     6241 aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga
     6301 accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat
     6361 gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc
     6421 agattatctg attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc
     6481 aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg
     6541 gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat
     6601 cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt
     6661 tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa
     6721 gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat
     6781 ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac
     6841 gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc
     6901 tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa
     6961 aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt
     7021 gcctgatgtc tatgttcctt gtgaagtatg tcatggcaaa cgatataact ctgaaacatt
     7081 agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaagatgc
     7141 tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt
     7201 tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca
     7261 acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact
     7321 agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca
     7381 aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa
     7441 aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct
     7501 tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt
     7561 gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa
     7621 tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc
     7681 tacactgatt ggtatagaca aaaacttcct ataagttcaa tccgttcaaa aaagaattga
     7741 gcaatcggag gagtttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag
     7801 ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa
     7861 atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt
     7921 cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta
     7981 cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtgt
     8041 ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc
     8101 ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt
     8161 cagaatattt tatctgtcca tatattgatt attctttatt tgaaagactg atcaataaag
     8221 taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca
     8281 gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg
     8341 ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag
     8401 cttttatttt agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg
     8461 gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg
     8521 tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgtctt ggtcatccac
     8581 tgttagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata
     8641 acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg
     8701 ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt
     8761 tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgtttcg
     8821 tcacagaaga tcgagtgttt aacaaaccct ttgttgaaac gacctatgga acgaatcaat
     8881 cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg
     8941 aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg
     9001 tgttttatga aaaagaccgg tcaatcaagc gctggttgtt gatgaaatat atgttccata
     9061 actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt
     9121 ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca
     9181 accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg
     9241 caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt
     9301 ttctgctgga tgatattgag aaacaaaatt tattgcagcc gaacttcatt gtgttgactt
     9361 gtaacaccgc acactacttt tttgaagaat tacaagcggc aacggatatt cctatccttc
     9421 atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga
     9481 ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg
     9541 gattcgaaac agtgattcct gatactgttc ttcaagaaaa aatcaattat ctgatctatc
     9601 atgagatcaa ggaatcggat tatttgaatc aagagctata ttacgaaatc ttagaagaag
     9661 cggtagagcg cctaaactgt gaaaaagtta tcttaggttg cacagaattg tcattgatgc
     9721 atgaatttgc tgaagataac cattatccag tgatcgatgc ccaatctatt ttagcagatc
     9781 ggacgattga acgagcgcta gctgaaagaa gcaaggcatt ggatacagca tcggaaaagt
     9841 aaaaaaattg gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa
     9901 agaaaaagaa aactggtaat gatacgcccg gaatgatata attttactat gcacaatgtg
     9961 agaggagttc gtaattatga cagataattt gcaattagtc atcataacag gtatgagcgg
    10021 tgcagggaaa acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa
    10081 tatgccgcct cgattgattc caaaattctg ggaattaatc aaagaatcag gcaaagtcac
    10141 aaaaatggct ttaggagtgg atttacgttc tcgttccttc tttgaagaaa tccaaaacat
    10201 gctgatcgaa atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc
    10261 agacgaagaa ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga
    10321 cggtttgatc actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca
    10381 agcttccgtg atcattgata cgacgacatt gacaccacga caattacgtg aaaaaatcaa
    10441 tgagtcattc aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt
    10501 taaatacggg ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc
    10561 tcattatatc ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt
    10621 gatgtccttt gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt
    10681 attgcctgga tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg
    10741 cggtcaacac cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta
    10801 taaagtcaat gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg
    10861 aaaatgaaaa cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt
    10921 ctgccagtta tcctgaaaag tttgcgaaat caaggagcag atatcacagc agtagtgact
    10981 gttgcagatg atgggggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct
    11041 ggtgacttga gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt
    11101 ttccaatacc gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg
    11161 atcattgccg cagtttccga gatgcgggga agcacttatg aggcgattca attattagca
    11221 aaaatgatgc atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat
    11281 gctgtcttta aagacggaac gactgcagta ggagaatcta aaatcgcgat cgaccgtaaa
    11341 accattgacc gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga
    11401 aaagtcgttt cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc
    11461 accagtatcc ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact
    11521 gctgaaaccg tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca
    11581 gatgccgatc atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta
    11641 ttggtcaata cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat
    11701 ctagtacaag tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc
    11761 accgactttt tggaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa
    11821 gaattgttcc gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag
    11881 gagaatagga tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat
    11941 cgagaacatg cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt
    12001 gtgaaccagc agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc
    12061 tattctcttt tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg
    12121 aaattaaaaa agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg
    12181 atggatcttg atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg
    12241 ggaaattcgc aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc
    12301 aataatccgg aaacgagccg ttatcatttg gaaatatttt ctatttatga acagcataac
    12361 caagacattt gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc
    12421 aatggctata tttcttattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt
    12481 ggtgcaacca actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat
    12541 tcggtcaatc gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct
    12601 tctaaacaaa tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct
    12661 gaaaaacttc aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa
    12721 ttaggggaaa tgattccctc aggtgcgata tcaaaatcgg gaattaatca ccgaattcgc
    12781 aaaataaatg aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact
    12841 gatacatgaa gcaggagatg cgaatgtatc agtttttttt aaaagttata cttgcttttc
    12901 ttccgtcaaa ctataattcc atctttactg gagtaacata atttggaaag gcttctattg
    12961 tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttgtac
    13021 ataaattttt gcttaatata gaagtcctga tatagataag atcaagtatt tatctataac
    13081 tggcctgatt aattaaaaaa ctggaaggaa ctgctcgatc atccttgaga actgctcttc
    13141 ttacttctgg tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat
    13201 tagctagtcg aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg
    13261 atttattcag tgataatcat aagttcataa atagtaattg agatgtatgg ttggaatacg
    13321 ttatacctga acttgaacta ccaagatttg gtttagaatt tgagaatcga tgtttataaa
    13381 gggagggcaa aaatgcgttt ttttacaact tttactatca ttatgattgc agtacttttt
    13441 atttttctag atatagcaaa gcgaaataca gcatttttat tatatcgggt tttacttcga
    13501 gcaggtctca taacatttat atcgatcgtt ggattttttc tattcacagt tattgtattt
    13561 atttggagaa ctccagcacc accattgcca gagattacat atggagaatt tccttttaga
    13621 cttgagtatg aattgaatga agaattacat gtgatagaag acacgttaat agttgaattt
    13681 gatggatttg gaatgaacga aggcataggg agatatcgta gatggacaag tcgtttagcc
    13741 agcggagaag atttagtgtt gctgttagaa gttagtgata ataagcaaat attttatttt
    13801 ccaggacctg ctaattatta catgggagat aggttgaatg gttataatca tacttttcct
    13861 agtgcttctt tcattgaaag ggaaagaggc attactagaa gggatatttt acatgacaaa
    13921 gaattattag aacaatttgg tccgcttgat caaaatacaa taaatgaaga agaattgtta
    13981 aatcaatata atataaggct agtgaattgg gaaatatctg aaccgattgt taacaatttt
    14041 ggtgattaga aaacaattac gataaataga aacaagccaa atcatcagca agtaaaagct
    14101 gatgatttgg cctttcttat acgagtagta ggaattcata gcgaatatta ttacgatcag
    14161 tagatatttt gatattaaag gagtattacg aactagtatg tgagaatgta gaggtgattt
    14221 gcgaataatg agaaatttat caaatagaaa caaaatatta atcattattg ttgttatagc
    14281 tgtgtttcat ctgggaacga atgcggtttt aagtagaata atattgggtc ctaagccacc
    14341 aagacctgcg attacccgag gggagtttga ttttagatta gaatatgaag tagatgggga
    14401 aaggattgtg atagaagata caattgttgc attatttgat ggattttctg cagatgcagg
    14461 tagcatggca tggtatcgca cgtggcgttt gcatttggcg agtgacaaga gggcacaaca
    14521 attattgtta catgagttag atgatggtcg aaaaatatat tatcattttg gaggagctgg
    14581 attttatatg gggaagttag tagcagtgta actgtaacaa ggaaagattt gccaggtctt
    14641 ggcattcgaa actccatatt ttatatcgag cgtgggagag atacatctat tgggaacagg
    14701 aaagggttaa ctcttgaaga gttatataat gactttggaa tccgtcttgt tagttgggaa
    14761 cacaatccac caatagaaaa tcgctttgaa taatcgaaat ctattaatcc atttcatcag
    14821 ccagtgaatg ttgtaatgga acaatttggt ttagtgttca aaaccattga aatactaggc
    14881 tgtgatcagc aaaaatgtat ctgaataaaa aagagagcaa ctattaaatg agaggagtta
    14941 ggagttgtta gctttatacg aatcaaagtg gcaaatacag atgcgtatgc atttgtgttt
    15001 gccatcttgc gtttggtaga cgagataggt ataagctaaa gaaacaactt gtttaaaata
    15061 aacgacctag ctcaacaaac gaccatctaa tcaactaaca gcatacataa aagcaactat
    15121 ttttgaagta gaattgaaat cgggataact taaaaagtga ttgggatact agtgtgaatc
    15181 accgtacata aatattactc gttaagggaa tttgagaaat tttgtatgaa aatttaagaa
    15241 ttagttagta tgcgcaagtc aaaaatatga ataaattatt tggtggagat aatttgctca
    15301 gcttgtcata agaaatgaga taatctggca gatgcaagct tttggacacc atttattcgg
    15361 aataaggagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct
    15421 tccgccgttt ataggtgttt agagcgtgag acaaaagtgt tctttacttt tgtcctacgc
    15481 tccttatttt tttattttgt ttggatcaat tcttttaatt cctctattga aatagattca
    15541 tcaaagattt ctttacccaa gatgacagtc ggaacaaatc gaatatgtgc ttgctcagct
    15601 tcgttgatga cagcttggct cagctgttcg tctttttgtt ctgtcagctt tagtttatct
    15661 actgcaaaat ccgctacttc ttggagagaa agatgtttcc actcgtcttg agtatcgaat
    15721 atttgtttga tctctttgat cgctttttga ccatcgctga ttgtgagata ccggtgcatc
    15781 acgtttcctc gctgcaagct ttctttttct ttatcgaaca atttgatcac tcgctgcaat
    15841 tgcccgcttt ggactgcttc ttctaatagc tcataagatt cttcaaacca ttgtcggcaa
    15901 tacggacaag ataggttgat gaattcgacc atttttttag gagcattcga tgaaccataa
    15961 aggattcctt tttgtgtatt tgttttcgtt gcatcaatca ctgatatatc catgttaaaa
    16021 cacctctttc gcttatagtt tatacatgtc tgacaaaaaa aggcaacgat ttagactgtt
    16081 tgtctaaaaa ttagatgacc aattcaaata gacatatgtg aaatattttt ataaataaaa
    16141 gaaaaaatgg aatgttttac gttataatgg aacagataat aagagagaat atgaacaagg
    16201 atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat acggcagccg catatatttc
    16261 ttatgcattt accgagattg ctgcaatata tccaataaca cccagttcga ccatggctga
    16321 attagtagat caatggtcag cagcaggaaa agaaaatgta ttcggtcaac cagtaaaagt
    16381 agtagaaatg caatccgaag caggagcagc tggtgtggtc catggatcat tgaaaactgg
    16441 tgcactgaca actacatata ccgcttctca agggctgctt ttgatgatac cgaatatgta
    16501 caagattgct ggggaactac tgccaagtgt tttccatgtt gctagtcggg cagtgacgac
    16561 aaatgcgttg aatattttcg gagatcacgg agacgtgatg gctgcaagac aaacaggatt
    16621 tgctatgtta gctgaaagca gtgtacaaga agtgatggat ttagcacctg ttgcccatct
    16681 tgcttctatt gaaggagaag tacctttttt gaatttcttt gatggattcc gtacgagtca
    16741 cgagattcaa aaaatcgaag ttttggaata tgaagaatta gctcctctga tcaatcagga
    16801 gaaattagct tcatttcgta aacgagggat gaatcccaat catccgactg ttagtggtac
    16861 gaaccagaat ccagacatcc attttcaaca acgggaaacg atcaacccct attatgataa
    16921 attacctgaa atcgttcaaa aatatatgaa agagatcaac cgattacgag gaacttctta
    16981 tgacttggtg acttattacg gagcagacga tgcggaagaa gtcattgttt ctatgggatc
    17041 agttgcacag acgattgaac aaacgattga ttacttgaat gcaaacggac ggaaaacagg
    17101 ttttttgaat atccatcttt atcgtccttt tccagtggaa acattcgtgc aaaagttgcc
    17161 tcatacagta aaaacaattg ctgttcttga tcgaagtaaa gaaccagggg caggtggaga
    17221 acctctctta ttagacgttc aaagtgcgtt atatgattct gaacttcgcc cggctgttat
    17281 tggtggtcgc tacggattgg ggtctaaaga tgtgactccc gaccaaatct cagcagtttt
    17341 tgatgaattg aaaaaagacc caagcatacg caaaaaacgg tttaccatag ggatcgtgga
    17401 tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg gatttgacag aaccgcaaac
    17461 attccaggcc aagttttggg gatttggatc tgacggaaca gttggagcaa ataaatcagc
    17521 tatcaagatt attggagatc acaccgataa atatgcgcaa ggctattttt attatgattc
    17581 gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt ggagatacgc cgatacgttc
    17641 agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac acaccagctt acctgcatag
    17701 ctatgacttg gtcaaaggat tgaagccagg tggcattttt ttattgaata ctctttggag
    17761 cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa cgttacttgg ctgaaaataa
    17821 cattagattc tatacgatca atgccatgag attggcccaa gaagtcggtt tgggacgccg
    17881 gatcaatacg gcaatggaaa cggctttttt taaattagct gacatcattc cttttgatga
    17941 ggtgcttccg ctgctaaaag aagaagccct gaaaagctat ggacataaat caatggctat
    18001 cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa ctgcttcatc aagtcgcgat
    18061 cccagaccat tggagaacgt tggagatgcc tgaaaaaatc aataaaagga acactggtta
    18121 tatacaagaa atacttgaac cagtgaatgc acaggaagga aatcaactct ctgttggtac
    18181 acttgtagca aatggaatga caactggcga aatgccttta ggaacggctt ctatggaaaa
    18241 gcggggcgtc gctttagaag tacctgagtg gattagtgat cgctgtacga tgtgtaatga
    18301 atgtgccttt gtttgtcctc atgctgccat ccgtccattt ttagcagatg aagaggaaat
    18361 ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga ggcgcagacg gcgtaaaata
    18421 ccgtatccaa gtttcagtgg aagactgtac aggttgtggt ctgtgtgtag aggcttgtcc
    18481 cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa cagaaagaac aagctgtgaa
    18541 ttgggctttt gcaatgacat taagacaaaa agcaaatcca gtcaaaggaa agaatactgt
    18601 cttgtctaca caattcaatc aaccgcttct tgaattctcg ggcgcttgct caggctgtgg
    18661 cgaaacacct tatgtaaaat tattgacaca aatgttcggt gatcgaatga tgatcgcgaa
    18721 tgcgacaggc tgttcttcta tttggggcgg tgcttctcct gcttctcctt atacgaccaa
    18781 tgaatgtggt caagggcctg catggtcaaa ttctttattg gaagataatg ctgaatttgg
    18841 ttatggtatg cttctagcgg cacagactcg tcgcgaggct ttggcaagtt caatcaaaga
    18901 agtattgcca aaagtatcag aagaactacg agaattgatg gaagactggt tggtacacat
    18961 gtttgagagc gaaggaacgc agcaaagagc agcaaaactg caagctgcga tcgaaagcga
    19021 acgatcaatt gtccctgaac tggaggatat atacaagaga aaggatctat tcgtaaaaaa
    19081 cagccaatgg atgattggtg gagacggatg ggcttacgac attggttttg gcgggatcga
    19141 tcatgtactt gccagcggag cagacgtgaa tattcttgtt ttggataacg aagtctattc
    19201 caatactggc ggtcaaacct caaaagcaac accagcttcg gcaatcgcta aattttcggc
    19261 tagtggaaag tatgcttcaa agaaagatct aggaatgatg gcgatgacat atggaaatgt
    19321 ttatgtggcg caaattgctt caggtgccaa tcaaatgcag acgataaagg cttttgaaga
    19381 agcagaaaag tatcctggac cttctttaat cattgcctat acgccttgta tcacacatgg
    19441 tttgcttggt ggaatgaaag aatcgcttaa agaagcaaaa gaagctgttt cttctggcta
    19501 ttggtcatta tatcgatata accctgcaat ggtcgaaaaa aacaagaacc caatgacttt
    19561 ggactacaaa aaacctgatt tctctgctat gcctgatttc atgaggaaac aagttcgctt
    19621 ttcctcgcta gagaacgcaa atccaaatct tgcaaaagct ttatttgaaa agactatcca
    19681 agacgctaaa acacgttttt acaactatgc tgcactgacg ggagaattag aaaaaatcaa
    19741 gaaacgactt gaaccagagg aatctatcga tgtacccgtt aagactagaa aagaaaaaac
    19801 gacggacctt gaagcagaag aaagaagagc tgccagagca gaacgacgta aaaaaatgga
    19861 acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat aattctctga
    19921 agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt tcctgaaaat
    19981 tatgcgtttt tctcaagaaa aatacgagca gatcagcctc taaaattaaa acatatgata
    20041 tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca aatcggggaa
    20101 ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc tctgaatttt
    20161 ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact gatccaactg
    20221 ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat tgttatccga
    20281 atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca agtcattacg
    20341 tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg actggaacat
    20401 cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga tgagaagatg
    20461 atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg tgcgctaatt
    20521 actatcgaac ggcatactgg attagatgag tatattgaga caggaattgc attagatgca
    20581 gatatcactg gagagctgct gatcaatatc tttattccta atacaccgct gcatgacggt
    20641 gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc tctatcagaa
    20701 agtatgttga ttccaaaaga attcgggact cgacaccggg cagctgtcgg tataagcgaa
    20761 gtcagcgacg cgattaccat cgttgtctct gaagaaactg gggatgtcag cattacatta
    20821 gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatttt gcgtagagaa
    20881 ctgattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt
    20941 atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc attctttaca
    21001 gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc
    21061 caaatacgct ttctggaaat gaagcgtata atcaaacagt tactggggtg ccaatcaaga
    21121 tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg actgtcaaac
    21181 tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca cggatgttcc
    21241 gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaggttccg ctaaaaattc
    21301 aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca gtaacggttg
    21361 agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat
    21421 ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc actacaggtg
    21481 acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg atggtcacag
    21541 atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa
    21601 gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag ccaagcaagt
    21661 cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa agctatgatt
    21721 tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt gcatcgatcg
    21781 gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa
    21841 ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt gtggagatta
    21901 cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg acacaagaaa
    21961 atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca tcgacaacaa
    22021 ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca gaagaaacat
    22081 cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat taagttaaag
    22141 gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa
    22201 caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag
    22261 ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata caagaatctc
    22321 aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta tcgaagtgtt
    22381 ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc aaaaagcaag
    22441 cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa tcaaattttt
    22501 cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag cattattaga
    22561 tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg tagaagaatt
    22621 tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg gtgacctttc
    22681 cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt
    22741 gtttgccgat cttgaaacag atttttatac catgggcaca aatccagacg ggttaaatat
    22801 caatgatggc gtaggctcta cccatccaga acgtttagct gagatggtcg ttgaaaaagg
    22861 agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg tggatgaact
    22921 tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctagatact tggcagaaaa
    22981 aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag gattccacaa
    23041 agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc gttacgttgt
    23101 agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc acatgatttt
    23161 cttagatttt aacacaactg gagatgggat gctttcagga attcagttac tgaatatcat
    23221 gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct atccgcaaaa
    23281 attagtgaat atccgagtaa gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa
    23341 agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag ttcgtccttc
    23401 cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga aagttaatta
    23461 ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt aagagatatt
    23521 ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt ccttgttctt
    23581 ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt cgaatccccg
    23641 taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta gagtagttag
    23701 ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc cctctgagac
    23761 aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc gagtatgcgc
    23821 accagtcact tcttcactga tgattccaat cactggtgtt ccttcttcga tcaaagcaat
    23881 cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac tgatttcttt
    23941 caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg ccacgtccaa tgtaaaaggc
    24001 atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt tttgggcgat
    24061 gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga atacaagaga
    24121 agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta cagctatttg
    24181 tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca atagcagtgt
    24241 aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa gtgaagggta
    24301 gccgagacgg ttgattttta ccagaacttg gcggctatct gctgtttcgc cactttgact
    24361 caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa attcactggc
    24421 taaatgaacc tcaacaggga tctgtgtcaa tttttccaga gtctgtttac ctgctaaccc
    24481 cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag acaatgtatc
    24541 gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt gagcgatacg
    24601 acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat aggtaccttt
    24661 ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa cgttctacga tgtcacctgc
    24721 aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat cttcaatctc
    24781 aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca cattgaatcc
    24841 atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa catcaggtgc
    24901 tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag cttttaaaaa
    24961 cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga taatctcagt
    25021 atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt cttcaaagtt
    25081 ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt tgggaagtat gtggatgggc
    25141 gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta tgaccaatac ctatatttcc
    25201 tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg ctttgaccag
    25261 ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac ggtattctaa
    25321 tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca ttccaacaat
    25381 tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct tccagctgca
    25441 ggaaaatcct tttgttttat ctttgcagat attttttgtg gattttctgt agagtgcagt
    25501 ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg tttacacaga
    25561 cctggcgcta tcctaaaagg tataactatc ctcctctttt gttttttatc tggaaacatt
    25621 taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa aataaattgg
    25681 tatatatcaa atgctttgtc aaatgctctt tttatgtcaa aaaataatgg gtttttatct
    25741 atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag gagtaaaaat
    25801 ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt ccaaattcga
    25861 agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct gggatagcat
    25921 ccaagaaatc gcacctgatg ccttggaagt tgctgaagct cgtgagttta cggtagaaga
    25981 tgaattgaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag agcggttcga
    26041 cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga aaggagaaag
    26101 aatgaataag agacgttggg cagcagttct gattgctgca ggattatttg tggtttcgtt
    26161 agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat tgaacagttg
    26221 gttatatggg gatgaagaac tagcttcaaa tgtgttggaa gagggagaca gtacgcaaaa
    26281 aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag gtttatttgc
    26341 ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg aagaagatcc
    26401 aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct acgaaagtgc
    26461 ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa tgtatgtcag
    26521 catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag ataaaatctt
    26581 tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat tgaattattc
    26641 aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag cgttgaaaga
    26701 tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc aagcatatat
    26761 cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata aatctgaaga
    26821 agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa aagaagtagg
    26881 tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatgcgaa aggaacaaca
    26941 gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg ctagtacatg
    27001 gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta gtcagatctt
    27061 atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt attacggagg
    27121 tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga taagcaacag
    27181 cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc ttttgaaaga
    27241 gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa gtatacggac
    27301 acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc aggttttttt
    27361 atccgaatgt ttgctttctt ggtcgatctt ttgtgtatcg gagctattac gcgtttaaca
    27421 cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct gagtatctac
    27481 ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa attcaatcat
    27541 ggtcaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga tgaagaagaa
    27601 cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct aaaatttcct
    27661 ttgctgatgt tagggtatct tccagcaatc tttaatcaga aaaagcaaca tctaggcgat
    27721 tttttcacta atacaagtgt tgttacgatc aatctgatca aagcattcaa ccaggaagtc
    27781 aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa agtcagatct
    27841 aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt ttttgttttg
    27901 ctactattga acatgagctg aacatgagca agaggaggac actgcgtgaa gccgattgat
    27961 tttccagaaa aatatgagaa tttaatgcga gtagcacaac aagcactagc aaaccagcaa
    28021 tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag ctttgaggct
    28081 aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc attgaagcaa
    28141 gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt ttattttgac
    28201 ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag tacagatttt
    28261 tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga attatccgga
    28321 caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact tccttcgttg
    28381 gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta tcatgagttt
    28441 atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc aagggcaaaa
    28501 ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt gacgattgaa
    28561 gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca ggcaagttac
    28621 cagcaattat gtgtattttc agatcgttac ggtaatgaag atgcgctgct tagttccgtg
    28681 ttaaatgaag aattcacgtt gcaatctgca attgtttatc ctgtctatga tacatacatc
    28741 gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa aacgatgtat
    28801 gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat tttgcagcaa
    28861 atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg aaaaacaagg
    28921 attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt aaaggtatgc
    28981 atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta aatgactgca
    29041 acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc gcaagacgaa
    29101 atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa cgtacctgga
    29161 ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga agaagcttta
    29221 tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt caaagaagct
    29281 ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa aggtcaagac
    29341 tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca atataaagat
    29401 ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc acgtatcaaa
    29461 cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc tgaaaatggc
    29521 gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga tggcggaaaa
    29581 ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt tgaagatcaa
    29641 ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc agaagattat
    29701 caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca tgaagtaaaa
    29761 gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga cgaagtatca
    29821 acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa agaaaaagca
    29881 gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc agaaatcgtt
    29941 gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga attcttgaac
    30001 aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg ttctacagaa
    30061 gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac aaatcttgtg
    30121 atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat caatgcagaa
    30181 atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg cgtgttgaca
    30241 gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat cacaggtaca
    30301 gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa gctacttcta
    30361 gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt ccttattttt
    30421 tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa cactttttct
    30481 ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt ttcttgcata
    30541 ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa tgagaccgta
    30601 cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc tggtccaggg
    30661 gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga agaattttat
    30721 gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct tgaagtattg
    30781 aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc cgtatacaat
    30841 cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga actgcaaaaa
    30901 agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc acaaacatta
    30961 gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac agaagctggt
    31021 tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc tgattacaat
    31081 gtggaacgtg cagaaaaagg gatcatctat attgatgaga tcgataaaat tgcgcgtaaa
    31141 agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca agccttgctg
    31201 aaaattctag aaggaactgt tgcaagcgtg ccacctcagg gaggacgtaa acatccacat
    31261 caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg tgcatttgat
    31321 ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt tggtacacaa
    31381 aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga agatttacta
    31441 aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc tttagagaaa
    31501 ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct agtcaaacaa
    31561 tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga agcgttgaga
    31621 gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg ttccatcatt
    31681 gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat cgaaaaagtg
    31741 attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta taaagattca
    31801 gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc ttttgcgaca
    31861 gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc atcaagctga
    31921 aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc cagaaatagc
    31981 tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga ttgatcgaaa
    32041 gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt tttatttgat
    32101 cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag tatccaaaac
    32161 tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag accagctacg
    32221 tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata tccagatgta
    32281 tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg cagataagat
    32341 ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg attttgatcg
    32401 caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag aagcgtgggc
    32461 agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca gtcaaactct
    32521 ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg tttcttgtct
    32581 tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc ttttggtgca
    32641 actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt atctgtacgt
    32701 tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc tatcatgtaa
    32761 tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcgtt ggttataatt
    32821 aaattataaa aagattttaa agaggtgaga aaatgattgg cgaagataaa ttattgatga
    32881 ctatccagct cacagactta aatgaagaag aaaaaaaaca ggaaataagc gattgggcta
    32941 ttttgacacg tctgttgatc cagccaacat ttatccgctc tttcacacaa caagcggatc
    33001 cgtatgatct tcgaaagctt caagaaaaaa taatgctagc atcggtaaga gacgaaagtt
    33061 ggttagttgt aggaaatgac gagaacgaat gtagttttcg cttggaagat aatcaattat
    33121 tgataaaaaa tgtattgtct atctctgttt tcaaagaaaa gcaatttttg attcgagact
    33181 tcattcagaa aaaaatgatt gctcatgggg tgttcgccta tatgcgagct tatagcgagt
    33241 ttatctatca caatacaaaa cagatctctc aacggctatt gtttgagaaa aaggatgaaa
    33301 tcgaacattt acctaaaatg aaacaacaaa acggggaagt agtagtcgat tgcaaccaat
    33361 ttccaggata cgatcttttt taccaaggtt tatgtttcac ttcgtgttgg gaaatgtatt
    33421 atagtcgcta ttatcatcaa atcatcccta aaccgatttt tctcgacatc caacaagtag
    33481 aaaaagtgaa ggaattagaa aacgaagtca tatgtatcca gctttatcga gaccccttta
    33541 actggcaaaa gccaaacaat caaatgtttc aatcctattt tagggaccag cttgggtttg
    33601 atcatcttgc ttgggacaat ggtgtcggtt tattgaaacc gccattcgtt gaatacattt
    33661 acactgacca tacgatccaa tctgtccagt atcagaatgc acaaatgcaa cctgttccta
    33721 aaaagaatgc ttcatttttt gtaacaaaaa gttatgatat tcaacaagga gattataaag
    33781 aaaggcgtgt tcgcggaaca ctaaatgctc aggcgtattt tccttgggtt gacgagaatc
    33841 gctcgcgcat gatgtgctat aaagttatcg atccgactgt tgctttggat aatggaatcg
    33901 aagcttattg ttattatatt agggaatatc tagaagtaga ggtaacagat gaaaaatatc
    33961 aagagtatct tttatcctta agaatctatg ttccttcaga atccctgtca gaattaccgt
    34021 taaaggaaat caaacataga ctctctgatg ttaccttcaa acgaataaag aaaaagcggc
    34081 gaagtatcca attcgatgtc aagaaaggtg agcagcatct gcgtgtgcaa tttttagatt
    34141 accgagaatt agaacaattg attactttac aaaaaatatg aggtgaagaa aatggataaa
    34201 caactctatt atcgaataat ggttccggca tcattattat ccgtctgtct cttttttgtt
    34261 cctaattttc ctattcgatc agtcatcggt tttttggtca tgtttggcgg gatgggactt
    34321 tattattatc ttcttcataa ggaaagaaaa cctgcttctg tttat
//
LOCUS       DAYYAG010000016        33405 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000016 DAYYAG010000000
VERSION     DAYYAG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.016"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            263..1819
                     /gene="rny"
                     /locus_tag="ACHARQ_000678"
     CDS             263..1819
                     /gene="rny"
                     /locus_tag="ACHARQ_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8409128.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1898..2704)
                     /gene="proC"
                     /locus_tag="ACHARQ_000679"
     CDS             complement(1898..2704)
                     /gene="proC"
                     /locus_tag="ACHARQ_000679"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8409129.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2845..5418
                     /gene="mprF"
                     /locus_tag="ACHARQ_000680"
     CDS             2845..5418
                     /gene="mprF"
                     /locus_tag="ACHARQ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8409130.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5572..6423
                     /gene="ispE"
                     /locus_tag="ACHARQ_000681"
     CDS             5572..6423
                     /gene="ispE"
                     /locus_tag="ACHARQ_000681"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8409131.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            6668..7606
                     /locus_tag="ACHARQ_000682"
     CDS             6668..7606
                     /locus_tag="ACHARQ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8409132.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7629..8309
                     /locus_tag="ACHARQ_000683"
     CDS             7629..8309
                     /locus_tag="ACHARQ_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8409133.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8282..9106
                     /locus_tag="ACHARQ_000684"
     CDS             8282..9106
                     /locus_tag="ACHARQ_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8409134.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9273..10091
                     /gene="purR"
                     /locus_tag="ACHARQ_000685"
     CDS             9273..10091
                     /gene="purR"
                     /locus_tag="ACHARQ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8409135.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10594..11967
                     /gene="glmU"
                     /locus_tag="ACHARQ_000686"
     CDS             10594..11967
                     /gene="glmU"
                     /locus_tag="ACHARQ_000686"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8409136.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            12061..13032
                     /locus_tag="ACHARQ_000687"
     CDS             12061..13032
                     /locus_tag="ACHARQ_000687"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8409137.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      13038..13140
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13206..13775
                     /gene="thiT"
                     /locus_tag="ACHARQ_000688"
     CDS             13206..13775
                     /gene="thiT"
                     /locus_tag="ACHARQ_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8409138.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13856..14629)
                     /locus_tag="ACHARQ_000689"
     CDS             complement(13856..14629)
                     /locus_tag="ACHARQ_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409139.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14896..16596
                     /locus_tag="ACHARQ_000690"
     CDS             14896..16596
                     /locus_tag="ACHARQ_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8409140.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16566..16784
                     /locus_tag="ACHARQ_000691"
     CDS             16566..16784
                     /locus_tag="ACHARQ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8409141.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16804..17694
                     /locus_tag="ACHARQ_000692"
     CDS             16804..17694
                     /locus_tag="ACHARQ_000692"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8409142.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17712..17996
                     /locus_tag="ACHARQ_000693"
     CDS             17712..17996
                     /locus_tag="ACHARQ_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409143.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18483..19097
                     /gene="gmk"
                     /locus_tag="ACHARQ_000694"
     CDS             18483..19097
                     /gene="gmk"
                     /locus_tag="ACHARQ_000694"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8409144.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            19102..19407
                     /gene="rpoZ"
                     /locus_tag="ACHARQ_000695"
     CDS             19102..19407
                     /gene="rpoZ"
                     /locus_tag="ACHARQ_000695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8409145.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19660..22068
                     /gene="priA"
                     /locus_tag="ACHARQ_000696"
     CDS             19660..22068
                     /gene="priA"
                     /locus_tag="ACHARQ_000696"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8409146.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            22083..22574
                     /gene="def"
                     /locus_tag="ACHARQ_000697"
     CDS             22083..22574
                     /gene="def"
                     /locus_tag="ACHARQ_000697"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8409147.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22567..23505
                     /gene="fmt"
                     /locus_tag="ACHARQ_000698"
     CDS             22567..23505
                     /gene="fmt"
                     /locus_tag="ACHARQ_000698"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8409148.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23505..24863
                     /gene="rsmB"
                     /locus_tag="ACHARQ_000699"
     CDS             23505..24863
                     /gene="rsmB"
                     /locus_tag="ACHARQ_000699"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8409149.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24876..25616
                     /locus_tag="ACHARQ_000700"
     CDS             24876..25616
                     /locus_tag="ACHARQ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8409150.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25613..27682
                     /gene="pknB"
                     /locus_tag="ACHARQ_000701"
     CDS             25613..27682
                     /gene="pknB"
                     /locus_tag="ACHARQ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8409151.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            27847..28746
                     /gene="rsgA"
                     /locus_tag="ACHARQ_000702"
     CDS             27847..28746
                     /gene="rsgA"
                     /locus_tag="ACHARQ_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8409152.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            28759..29409
                     /gene="rpe"
                     /locus_tag="ACHARQ_000703"
     CDS             28759..29409
                     /gene="rpe"
                     /locus_tag="ACHARQ_000703"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8409153.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            29410..30048
                     /locus_tag="ACHARQ_000704"
     CDS             29410..30048
                     /locus_tag="ACHARQ_000704"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8409154.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            30203..31057
                     /gene="mreC"
                     /locus_tag="ACHARQ_000705"
     CDS             30203..31057
                     /gene="mreC"
                     /locus_tag="ACHARQ_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8409155.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            31061..31570
                     /gene="mreD"
                     /locus_tag="ACHARQ_000706"
     CDS             31061..31570
                     /gene="mreD"
                     /locus_tag="ACHARQ_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8409156.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            31825..33399
                     /locus_tag="ACHARQ_000707"
     CDS             31825..33399
                     /locus_tag="ACHARQ_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8409157.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        11012 a         5973 c         7225 g         9195 t
ORIGIN      
        1 taatagaaaa gtttctcaat ttaaaggttt acctatatga aactagtgtc atttatttta
       61 aaaaccatcg tttaatgaat aagtagaaag ccgtttcttt gtttatttaa agttgacact
      121 acggggtaca aacattagaa taaagttgtg catgtttttt gtacacatat atgcgaagat
      181 gcatattgac gagtttagaa taatttatca tagaagctgt atagatgata aaaatgaaaa
      241 agatagtatg gaggtggatt ttatggtatt caacattctc ctcgctatca tcggtttaat
      301 tgtcggtctt ggtttagggt ttatggtcgc aaagtcacgt cacgagaaag aaatagctgg
      361 tgcacaatca tcagctgctg gaatcatcga tagtgcaaaa aaagaagcag aaactctaaa
      421 aaaagaagca ttgttagaag ctaaggaaga aaatcagaaa taccgttctg aagttgaaag
      481 tgagctgaga gaaagtaagt tagaattaaa atctcaagag aatcgtttga ttcaacgcga
      541 acaaacgctg aatcgtaaag atgactcgtt agaaaaacgc gagaactcac ttgaaagtaa
      601 agaagaagca cttagttcaa aacagcaatt aattgaagag cgagaaaaag acgttgaaaa
      661 aatgattgaa gatcaacaaa aagaactaga aaaaattgct ggtttatcaa aagaggacgc
      721 acgagatgtc atcatgaaat cgactgaaga agagctgaat catgagttaa ctctaatggt
      781 aaaagaatct gaacagcgtg ccaaagaaga agcagatcgc aaagcgaaaa acttattatc
      841 attagcgatt caacgctgcg cagcagatca agtttcagaa acgactgttt cagtcgttac
      901 ccttccgaat gatgaaatga aaggtcgtat cattggacga gaaggtcgta atattcgaac
      961 gcttgagacg ctgactggta tcgatttaat catcgatgat acaccggaag cagttgttct
     1021 ttccggattc gatccaatcc gaagagaaat cgcacggatg accttggaaa aactgataca
     1081 agatggacga atccatccag cgcgtatcga agaaatggtc gagaaatctc gtaaagaaat
     1141 ggatgaacgt attcgcgaat acggggaaga ggcagcgttt gaagtcggtg ctcatacact
     1201 tcatccagat ctaatcaaga tcatcggacg tctgcgattc agaactagtt atggacaaaa
     1261 cgtcttgaag cattcggtcg aggttgcaaa attagctggt atcttagcag cagagcttgg
     1321 cgaagatatc caattagcaa aacgtgccgg gttgctccat gatattggga aagcacttga
     1381 ccatgagatt gaaggatctc acgttgagat cggtgcggaa ttagctgcca agtacaaaga
     1441 aaacccagta gtaatcaacg cgatcgcttc tcatcatgga gatgtggaag ctacttctgt
     1501 tatctctgtc cttgtagctg cagcagatgc gctctcagca gcaagacctg gtgcgcgaag
     1561 tgaatcatta gaaaactaca tccgtcgttt aagaagtctg gaaaatattt cgaacagctt
     1621 cgccggtgta gaatcaagtt acgcagttca agctggacgc gaagttcggg taatggtcaa
     1681 gccagaagaa atctcggatt tagacgctgt tcgtttagta cgtgatattc gtaaacgaat
     1741 cgaagaagaa ctggattatc ctggacacat caaagtaatc gttattcggg aaactcgtgc
     1801 aactgattat gctaaataat aaaaaataca aaagaagagc cgcggcaaaa gccacagctc
     1861 ttcttttttt gtaaggcacg aaaaaacatt gctatcatta ttttttattc aacatctcaa
     1921 gatctttctc gatcgtcgta ttgattcctt tgatgattgc tgggatgaat cctgcttctt
     1981 ctaaggcaac tacacctgca acagttgtac cgccaggaga agaaacttgg tctacaagtt
     2041 cccatggatt tttatcactt gaaaggaatt tttgacaaga acccataatt gcttttgcag
     2101 caatctcagt ggctgtctgt ttggacaaac cattcagtac accagtccgt cccatcgcat
     2161 cgatcagcat gtaaacgaat gcgggtgagc tgccagcaag gccaatgaat gtactgaaat
     2221 ctgattctgc taaaggatag acgctgccga ttgcttcaaa aatcatcgta gcttgctgaa
     2281 gctgttcctc cgtggcagct tcatttttgc aaatcgcaga aacgcctgct cctacgctga
     2341 cattgacatt cggcatcaca cgtacgatgg aggcctgagg atcttcaagc gctgtttcta
     2401 aatctattaa tgattttcct gaagcaatag aaatgaataa gactttctgg ctatattctc
     2461 ttagttcttc caatactttt tcaatgattt ttggtttcac ggctaaaaga acgatatctg
     2521 attgtgatgc aacttcctga ttacttttgc atgcatggac gccagtttca gcagcaaatt
     2581 tttgtgcatt ttcaaaatgt ccactggaaa cgaacacctc ttctttcgag actgttcctt
     2641 tttttaataa accatcaata atagcttgtg ccatattccc tgtgccgata aatcctactt
     2701 tcatgttatc ctctccttta gcattaggat atctttcttt ttctatcatc gcaagcaaag
     2761 aacaggtcta aagtggaaaa attaaaataa ataagataac attggggtat gacacaaaag
     2821 tatacaagtt aggaatggga aaatgtgaaa aataaacgat acttgatgct gcaatggata
     2881 aaggaacata gtctgatttt aaaactgatt tttttcggct ccgtgttagt ctttgtggca
     2941 aatcaagtcg caaatattgc taatggcatg agttggcaag atatttggca aaggatggga
     3001 caacaaagcc gcatgacgat cctgctcatg attctggctg gactgctggg tgtcactccg
     3061 atgttgttgt atgattgggt gacgatcaga gtcttagaaa aacaaggaaa accaaaaatg
     3121 gaaagaaaag agtttcttct tgctgcgtgg gtaacaaaca cgatcaacaa tctcgccggt
     3181 tttggtggtg tggtaggagc aagtctgcgg gctagttttt atggaaagca gacaaataga
     3241 aaaatggttt tagccactgt atcaaaagtc gcactattta tgctgactgg tttgtctgta
     3301 tggtcgtttc ttacatttat tgatgtcttt tttattcagt cagaaagtat ttttcgaagc
     3361 tattggatct ggcttttcgg cggtagtttt ttagcaccag tcattttctt ttttgcttat
     3421 ctaaagagaa agacattgtt cttcgagttt tatccaaaag gcttgcttgg tctgctactg
     3481 gcatcctttg gacaatggac gggagccctt gcggtatttt tgatcatcgg caagttgatg
     3541 agaatagata tttcgatgct ttcgatctat cctatgttca tgattgctac attgatagga
     3601 atgctgacga tggtcccagg tggaatgggg acttttgacg ttttgatgat cttaggaatg
     3661 agtcaattgt ctgttcggca agatgtagca gtagtttggc tattatatta tcggttgttt
     3721 tattatgttc taccttttat gacgggactt ctattgttca tcacacatac aggagtcaaa
     3781 atcaatcgat ttttagataa cattccgcga ctgtttttcc aaaaggttgc gcatatcgtg
     3841 ctcgtaagtg ctgtctattt tgcggggatc atgatggtac ttctatcaac aatcactaat
     3901 ctgtctgcaa tcagtcggct atttcaagta ttattgccgt tctcattcaa ttttttagat
     3961 cagacagtga atctattggt tggcttttta ttactcggcc ttgctagagc actttcagta
     4021 aaagtcaaaa aggcatttct gcctacgatt gctttactat cttttggtat tttaaataca
     4081 atcacacgta cgttatcttg gcagttgata ttggtatatg tcttgattct gttagctgtg
     4141 tggttatcca gaaaagaatt ttatcgaaca aaattcgttt attcttgggg agctataatc
     4201 tttgatgcta gcttgttcgg ctttttgttt atcgtttacg caattgcagg ctatcacagc
     4261 ggacgctttt tagtcggtcg aataacagga aatcatttta ttttgtttcc ttcagatgat
     4321 gtgtggttct cagggcttat tggtttgggc atctcattgc tagctttagt ggcgctttat
     4381 caatatttag ctgaagatac tacgaagctt ggcagtgagt acgaaaaaga ccgttttgac
     4441 cgtttattgg aaacatacgg aggcacagct acaagccatc agcttcgctt gtcaggttac
     4501 agttattttt attaccaaga agacgaccaa gatcaagtcg tttttggtta tcagatcaaa
     4561 ggaaacaaat gctttgtttt aggagatcca gttggcaatc ctaaaatgat gcgggcagca
     4621 acgaatcagt ttatgaaaca agcagattgt ttaggctatc agcttgcgtt ttataagata
     4681 tcagaaagct acgtagtgat gcttcatgaa ttgggttttc acttcacgaa agtaggtgaa
     4741 gcagggattg ttgatctagc aacagaagac ttgtctcggc tgagtcagca acttgaatat
     4801 caacgtttga tgaaagaagg ataccatttt acttggtacg atcaactgcc ggaagaatta
     4861 catcaagctg taaatgaagt ttcacaaaaa tggcttcaag gtgcacgaga aaaatacttc
     4921 tctgtgggac gatttgataa atcatatgta ttatctagcg gtgtcggtat tgtccgtaaa
     4981 gatcaaaaag tcgttggctt catcaccgct cagccgatga cgaagaagca ggcgggatat
     5041 gatttgttgc gggtcttacc taaagaatct gaggaattag ctgactatct gttagccaat
     5101 ctgtttattg tatacgagaa aatgggctac tcggaagcca atctaggctt agctccatta
     5161 gctaatgttg gagaaacaga tttttctttc tttcaagaaa aagtcatgca tatcgtatat
     5221 aattatggta attttttcta ttccttccag tctgcttatg aaaagaaatt aaaatatgtc
     5281 gatcattggg aaggacgtta ttttgcttat aggaaaggaa gtaatttcgg atttgctacc
     5341 atgcagctac tcagtctgat aggaaaagaa aaaggaaatg ttccgagttt ggcagaagaa
     5401 gtgctcacag aattataaga atcactagaa cttgtttgga ttatgaagag aaaattctgt
     5461 gaaagctgtt gaaacgatga caaaatcaac cactaggcag acataagcta tacttttatt
     5521 tttccctatg atagaatact tgcgacttag acttttcgaa tgggtgagag gatggaattg
     5581 attgaaaaag cgccagccaa gataaattta ggactggatg tattatatag acggcaagat
     5641 gggtatcatg agttggaaat ggtgatgtcg agcgtagact tggcagatca tctatttttt
     5701 gaagaaataa cagaagacaa aatcattgta gaaacaaata aagcattttt accggtagat
     5761 gaacggaaca atgtttatca agctgcttta ttgctaaaaa ataaatacaa tatcaaaacc
     5821 ggtgtcaaga tctttgttaa gaagaatatt ccagtagcag ctggattagg cggaggaagt
     5881 acagactgtg cagctgctct aagagggatc aaccaattat ggaatctggg ctgttcgtta
     5941 gaagaactgg cagaaatcgg tttggaagtc ggtacagatg ttccttactg tgtctacgga
     6001 agtacggcat ttgtcggtgg caaaggggaa gaaatccaag tattaccttc tatgccacag
     6061 tgctgggttg tgttggtcaa accaagaatg agtgtctcta cacgtacagt gtttagagaa
     6121 gtagatacgc aagccatcca acatcaagac atccacggac ttgctgaagc catccgagag
     6181 caagattatc agcagatgat caaaaaaatg ggaaacagtt tagaagatat aacgggaaag
     6241 cggcatccag tcatccatca aatcaaagag cgtatgatca agtacggagc agatgctgca
     6301 ttgatgagtg gaagcggacc aaccgttttt gccttgtgtc aacattattc aagagcgcag
     6361 agagtatata atgggttaaa aggtttttgt gaggaagttt atttagtacg aaccctgcaa
     6421 taaaatataa gaagtaaaat aggtgatgaa aaactgagga atcctaaaat ggtattcttc
     6481 agttttttat tgtagtgaaa tggctagata tgagcagcaa ctcatccatt ttacgattta
     6541 atcaagaata tacatgctta aatcagaaat tgatggattt ctattgactg tgttttttta
     6601 ttttgataca ttagttcacg ttaataaatc gtaaaattcc gatttgttca ttgaaaggag
     6661 catataaatg aaaaaatatc ttttgacagc tacggtaatg ataggtgctt tactgtttgc
     6721 agcatgtgga aatacgaaca aggaagcaga caaaaaagag gatttgacga tcgttacgac
     6781 tttttatccg atgtatgatt ttacaaaaga gatcgtaggg gatgaaggaa acgtgaaact
     6841 tttgatccct gctggaacgg agccgcatga tttcgaacca agtgcaaaag aaagagcaga
     6901 aatttccgat gcagatgttt tcgtgtataa tagttcagac atggaatttt ttgtggattc
     6961 gttaaaggat tcagtggata gcaaacagac cttgatgatc gaagcagcta aaggaatcga
     7021 ccgtttagaa tcgcaagaag cagacgaaca tgaagaatca gaagaagggc atggtcattc
     7081 acatgaatac gatccacatg tttggctcga tccggtatta gctataaaag aagtcagaac
     7141 gattgctgaa gaattaggcg aaaaatatcc agataaaaag gaaactttta cgaagaatgc
     7201 cgatgcttat atcaaaaagc ttgaagcact ggatcaaaaa tactccgatg agctaaaaga
     7261 tgcaacaaac cgcacatttg tcactcagca tgctgctttt gcttatttag cgaatcaata
     7321 cgacttaaaa caagtagcga tttcgggcgt ttcaccagat caggaaccca caccaagtcg
     7381 cttagctgag ctaaaagaat ttgtgaagaa aaacaacatc aaagtgattt attttgaaga
     7441 aaatgcctct tcgaaggtag ctgagacatt gtcgaatgaa acaggagtga agctagaagt
     7501 gttgaatcct ttagaaagct tgactaatga acaaatcaaa gcaggcgaaa attatatttc
     7561 tgtcatggaa aaaaatctga aagctttaaa agaaagtatc aattaagttt tagaatggag
     7621 gttgtaaggt gcgttatatc gaaatatctg atttgacctt ttactatgat gatgaacctg
     7681 tactagaaga tgtctcttat catgtagatg cgggtgaatt tgtcatttta actggagaaa
     7741 atggagcagc caagtcaacc ttggtgaaag catcgttagg attactgaaa ccttcaaaag
     7801 gaatgataaa gattgctgag aaaaatcaag aaggaaaaaa attaagtatc ggttatattc
     7861 ctcaacaagt tgcatctttc aatgcaggtt ttcctagtac ggtgatcgaa ttagtccgtt
     7921 caggaagata tccaagaggc cgctggttca agcgtctctc tgaaaaagat catcaacatg
     7981 tacagcgtgc tttagaagct gtagggatgt gggatatgcg aaatcggcga ataggtgaat
     8041 tgtctggcgg acaaaagcag cgaatcagtt tagcgcgtat ttttgctact gaccctgact
     8101 tatttgttct tgatgaacca acaacaggta tggatgaaga atccagaaat gaattttatc
     8161 gtcttctccg acatagtgcg catgtccatg ggaaatcagt tctaatgatc acccatgatc
     8221 atgaagatat caaacagtat gcggacagac agatccgact ggtgagaaag gaggattctc
     8281 aatggcgttg ctttcatatg aattcatgag aagagcgttt cttgccgcta tcttcattgc
     8341 cggaatcgcc ccgatgctag gtgtgttttt agtgatcaga agacaatcgc tgatggctga
     8401 tacgctttct catgtctctt tagcaggggt agcgttagga ttctttttga accttaatcc
     8461 tacacttacg accatgctag tcgttgtatt agcagctgtg atattggagt atctgcgaac
     8521 gatctaccga acctattcag aaatctccat tgccatttta atggctggtg gtttggcgct
     8581 agcgttagta ttgatgaatt tgagcggagg gaattcagca acaagtatcc aatcctatct
     8641 gtttggttcg atcgtgacca ttacatggga gcaagtcatc tttttaggaa tcttattcgt
     8701 tgccatcggt atattgttct ttttattcaa acggccgatg tatgttctga cttttgatga
     8761 agataccgcc cacgtggatg ggctacccgt tcatttgatg tcgatgatgt tcaatgtgat
     8821 tactggtgtg gctattgctg tgatgatccc gattgccggt gcacttttga tatccgcaat
     8881 catggttctt cccgcagcaa tcggtatgcg attaggcaaa agcttcaatg tcgtgattgc
     8941 tatcagtgtt tgtgtaggat ttctagggat gctgtcagga ctaactagct cgttttatct
     9001 agatacacct ccaggagcaa caatcacatt agtctttatt gctttgttcc tgattgtaaa
     9061 tatcatcaaa cgaatattgg ttgccttcag aagaaaaaat aactaaatta taaagaaaag
     9121 aagccttata aaaacgaaaa attttttata ggcttctttt tattttaata aataccgaac
     9181 tattttctta ttttttcata aaatgctcga atttttgaag aaaaacttat ataatgtaaa
     9241 acgataaaat ttcttaaaag gagttggaat aggtgaaagt acgtcgcagt gaacgtctag
     9301 ttgacatgac acagtattta ttgaatcacc cgcatgaatt gatttcctta acaagctttg
     9361 ccgaacgtta tgaatctgct aaatcttcta tcagtgaaga tttgggaatc atcaagaaaa
     9421 catttaaaga acgagggtat ggaaccttgg aaacaatccc gggtgctgca ggtggtgtat
     9481 tgttcatccc tgaaatttca tatgaagagg cgaaaaaata tattgaatct ctagcagaac
     9541 gtctatcaga acaagaccgt ctgcttccag gaggatatgt ttatctctct gatttgttag
     9601 gagaacctga actgctgcgt caagttggaa gaatcattgc ttctaaatac cttggtgaaa
     9661 aaatcgatgc tgtaatgact gtagccacaa aaggcgtacc tattgcccaa gctgtgtcct
     9721 attatctgaa tgcgccattc gttattgtac gccgggactc aaaaataaca gaaggatcaa
     9781 cagtaagtgt caactatgtg tctggatctt ctgaaagaat tgaaaagatg gaactgtcta
     9841 agagaagttt gaaacgtggt tcacgtgtcc ttgtagtgga tgatttcatg aaaggcggcg
     9901 gaacagtcaa cggaatgaag agcttgattg aagaattcga atcagaactt gttggcatca
     9961 ctgtatttgc cgaatcaaaa ttcaacggtc atcgcgccat cgatgattat acgtcactgc
    10021 tttatgtcaa agatgtagat actcaaacga aaaatattac ggttgtgcca ggaaattatt
    10081 tttcagaata agaggttgtg aaaaagccgc ttagtttcga gtagtaagaa aaattttgtg
    10141 aaaatggctt tccacatttt ttcataaatt tggcttaata ccgagaaact ggcttttgaa
    10201 caccgtttat ctagaggttg tgaaatcagc gtcctgtcct gagtattagc tcaataaacg
    10261 gaaaaattgc tcctcatatt ttgccgttta ttgagctaat accgaaggtc agctgattga
    10321 aaaacgttta atgaagaggt tgtaagccta ccgtttaact ctaagtcaca agaaacgatt
    10381 tcaaaaaaca gcttttcatg ttttatgagt agtgtgactt gtgaccgagg agttggcagg
    10441 agagcaccgg ttaccaaata gataggcccg ttatggtcta tctatttttt tatccagaaa
    10501 acgctgggat aaaagaaaat cacttgtttt gttgaaaaaa tgggtatact gggataaact
    10561 gaaatcagca aactatattt aggagtgacg cagttggacg cacgttatgc aattattttg
    10621 gcggcaggga aaggtacccg catgaagtca aaattatata aagttttaca tcccgtttct
    10681 ggtaaaccta tggtagaaca tatcattaat cgggtcagtg aaacgaaacc agatgaagtg
    10741 attacgatcg ttggacacgg agcagaacaa gttaaagcac aactaggaga acgcagtaaa
    10801 tatgcgttac aagcagaaca attgggaaca ggacatgctg ttttgcaagc tgcttctttc
    10861 ttggaaggaa aaaaaggaac aacgttagtt atcagtggcg atacaccatt attgactact
    10921 gaaacgctga acaatctttt tgaatatcac caaggaaaaa atgcaagtgc aacgatcttg
    10981 acggcacaag cagaaaaccc aacaggatat ggacgaatca tccgtgacca tattgggatc
    11041 gtagaaaaaa tcgtggaaca aaaagatgca acaccagaag aagctcttgt acaagaaatc
    11101 aatacaggaa cttactgttt tgacaacgaa gctttgttcg atgcgttaag caaagttgga
    11161 acaaacaatg ctcaaggtga atactatcta acagatatca tcgaaatctt aaaagaagaa
    11221 ggccatacag ttgctgctta tcaaacggat gattttgaag aatcaatggg agtcaatgat
    11281 cggatcgcgt tagctaaagc aaatgagatc atgagaaagc gaattaacca gatgcatatg
    11341 gtcaatggcg taagctttgt cgattcagca acgacataca tcgatgcagg cgtagaaatc
    11401 ggtccagaca cattgatcga agcaggtgta caaatccaag gaaatacagt cattggttct
    11461 gattgtgtca tcgggtcaca ttcaaaaatc gtagacagta ggattgagga tcacgtcgtc
    11521 attgaaaact cagtcattga aagcagccat gtcaagaaac atgcagatgt gggaccttat
    11581 gcacatttac gaccaaaagc tgaaattggc gaaaacgtcc atatcggaaa ctttgtggaa
    11641 gtgaaaaatg ctcaaatcgg caaaggaact aaagtcggtc acttgactta tgttggtgat
    11701 gctacgctgg gagaagaaat caatgtcgga tgtggtgttg tcttcgtcaa ctatgatgga
    11761 aagaacaaac atcatacgac tgtcggcgac cacagcttta ttggttctag cacgaatatc
    11821 atcggacctg tcgaggtagc taaaaacagt tcgattgctg ccgggtcaac aattacagac
    11881 aatattccag aatatgcgct ggcaattgcg agagcaagac aagtcaataa agaaggatat
    11941 gcaaaaaaac tcccttatct aaactaagac ttttgcaaaa tcacttgatt tatttaaaaa
    12001 atatgactac tatttattag gtagaattct agagttgtga aaaagaaagt ggaggtcccc
    12061 atgtcaaaac attactttga cccaagatta aaaatttttg cgttaaattc caatcgccca
    12121 ttagcagaga aaattgcagc agcagtcggc gtcgagttag gaaaatcatc tgttactcaa
    12181 tttagtgatg gagaaattca agtaaacatt gaagaaagta ttcgtggatc gcatgtctat
    12241 gttattcaat caacgagcag cccagtcaat gataacttga tggagttgct gatcatgatt
    12301 gacgcactga aacgagcaag tgcaaaaaca atcaacgtag tcatgcctta ttatggctat
    12361 gcgcgtcaag accgtaaagc acgtgcacgg gaaccaatca cagcgaaatt agtagcaaac
    12421 atgattcaaa aagctggtgc aacaagaatg ctgactttgg accttcatgc agtccaaatc
    12481 caaggtttct ttgatattcc agtcgatcat ttgatgggtg cgccacttat tgccaattat
    12541 ttcttagaaa aaggaatcaa aggcgatgat gttgtcgttg tttcaccaga ccatggtggt
    12601 gtcacacggg cacgtaaatt agcggaattt ttgaaatctc cgattgcgat catagacaaa
    12661 cgccgtccaa aagcaaatgt tgcagaagta atgaatatca ttggacaagt caaaggcaaa
    12721 acttgtgtac tgatcgatga tatgatcgat acagcaggta cgatcacatt ggctgccaat
    12781 gctttgaaag aagctggtgc aactagtgtg tatgcttcat gtacacatcc agtattatcc
    12841 ggccctgctt tacaaagaat agaagattca gctatcgagc gtctagttgt tacagactcg
    12901 atctatcttc cagaagatcg taaaatcgac aaaatcgatg aagtaagtgt tggcggacta
    12961 atgggagatg caatcaaacg tatccatgaa aacaaaccag ttagtccatt gtttgaaaca
    13021 aaaaataaat aataaataaa ctcacaaagg ggagtccaat tgggctgaga ttgaatctat
    13081 ttctaaaccc ttcgtacctg tatcggttat gcgagcgtag gaattgtgaa taaacagctg
    13141 tttgactatt ttcgaaagct gcttcctcct ttgtgacttt tttattcaaa ggaggaagtt
    13201 tttttatggg aaaaaataga gtttgggtag aaggaacgat tgttgcagcg ttagcgatgg
    13261 tgttgtcgtt gattccgatc cagatcggca gcagtttttc tatttcatta gggcagattc
    13321 cattgactct ttttgcgtta agaagaggga caaaggcagg gatgctggca ggtttcattt
    13381 ggggcatcct acattttccg ttgggacaag tttattattt aagtgtcgtt caagtgttga
    13441 ttgagtaccc gattgcctat acttttgcag gcgcagcagg gctcatggct tcaaatgtcc
    13501 aaaaaggcta tgtagacgat aatacgaaac aaattagaaa aagcatcgtg tttggtgcat
    13561 tgattggcgc actttccaga tatttttggc attttattgc aggagtggtt ttctggggag
    13621 cgtatgcact atgggggatg aatccttggc tcttttcttt cgtaatgaat ggtgccagcg
    13681 ggttgagtac agctattgta acgatcatcg ttttattagg gatccaacaa acgactcctt
    13741 cgatttttgt gccagataaa cgacaggagc tgtaaataaa aaagaagcaa gaaacttgag
    13801 acagaatcat gtcctagttt cttgtttcct ttttattttt tgataagaaa tagatctact
    13861 tcaatggcag ataagaaaga tcggcatctt ctaccaattg ccagtgtcga tcggtgacag
    13921 agtagattaa agtagcgatg atttcctctt ctcgataccg ggtgcctttt aatttccaaa
    13981 tataatcttc aatcacatag attgggatac gatatttacc atcgatcaaa ccaacttgat
    14041 caaaagaaaa aatgatccct tcctgcatca aattacgcat ttctttcaat aattccacac
    14101 tgattttttc aacaggtgtt tcttttttga aaaaagattt ttttacattg atcttatctg
    14161 acaagatcga acttaattta ctagcaaaaa tcctataaaa ttcatctaaa tagcgataat
    14221 aaatccgatt cagctgatca ttaagatcga agtatacaaa attattctgt agtttataaa
    14281 aaaacggcga atgaagatgt gttttcatat gaccaaaata taataattct gaaatttcta
    14341 aaggtgtcaa ttctttcagc atcgttaaat cagtaaagtc gatccacttc aattcatcga
    14401 atccacgcct gttacgagaa gaggaaaaga aacgctgaat attttctgta ccacggatta
    14461 ctttcagccc tgtatgggat tcatattctc cagcagaagc agatggatca agcaagagca
    14521 agttttgagg aaaatgcacg atactgtttg caaagtcagt agcagtcagc ccttttgtta
    14581 aaacagcatt acttgtggta tcaatatgta cataaactga ctcagtcatc atgttcctcc
    14641 tcttatttcc tcttaatctc ttttatttta caacatttgc attaaaaaag cttgtttctt
    14701 ttattttaaa attccattac aaaatcacgt tttcattgaa aacactctca ttttcaatta
    14761 atttttgtga tttttgtatt tttttattta aaggaggttt tatgaattgt tttcttatgg
    14821 aatctacaaa gtcgaatacg agactcatga cctttttatg acgtactggt atacttgaag
    14881 aaaaaggaga gcgcgatgga gataaaaaaa gtttactttt cacagaaaat gtcgctgtat
    14941 tcgcctatcc agcaagcaaa acaacccctt ccattttttc aaagtcatca agaaagtaga
    15001 gacgcttttt ggcgttctgc aaaaaagcaa tgtgctttta aagcagtatc gaaaaaatat
    15061 accgtctatt ttactattgc taaaagtaga ggagatgtta tttttaataa tctgttgatt
    15121 gaaggtgaaa cttttgaatg gaaaaaattt tttcggtata tggaagcttg cgctcgtttt
    15181 tttattaaag aaaactgttg ttttttattt caatctcctc tatcagaaga atggttgcgt
    15241 atttttcata aaaacggcta tcaaggaacc acacaattaa ataaaaagct ggtctatcat
    15301 acagcacttg ttttaggtag aggaggagcg cacggtgctt atcagattgg tgtgtggcag
    15361 gcgctgaagg aacatgatat caactttgag attatcacag gtacctcggt aggtgcgttg
    15421 aacggcgcat tgattttaca aggagatatg aaaaaagctg tgaatttatg gaaaaagctg
    15481 tcgacaagac aagtgcttgc gttaccagaa atggctgcag ttgaagacct tcgtgaacgc
    15541 ttctatcgag aaagacgcca aatgacgaaa acagcactaa ttgaaggcgg ggtttcttct
    15601 gctccattgg aaaagatggt caaagacaat ttggatttgt ccttactcaa acataatccc
    15661 aaaatacgtt tatatacggt gtctactaaa ctgccagaac tagcggaagt agtgactgat
    15721 atccaacaga caaaaacaga agaaatacca gattggatac tcgcctctgc ttcattttat
    15781 cccgctatgg cttatcgaaa gatcggaaaa aataaatatg cagacggtgg gtatcgcaac
    15841 aaaataccta ttgatattgc gattaacaaa ggagcaacag aggcatttgt cgtagatgtg
    15901 caagggccag gcccagcgaa aaaaattcgt atgccagaca tttttatcca ttggaaatgt
    15961 caaacattgt ggacactagg cagctttctg ctttttgata gccaaaggaa ccaattaaat
    16021 ttacaattag gctatctgga aatgaaaaaa cgattaggct attactatgg gaactggtat
    16081 acatttcatt ctgttaaacc tgcaagaaca tactggcgag gatttttgtc ttacttaaca
    16141 aaagaaattc agttagaatt atcttttttc aaaagcataa aattttggca aaaactgcgt
    16201 aatttatata agaacagggt cgtaccagag acatgcggat tggcgatgct agaactttta
    16261 gcaaaaaaac attttttact gcccaatgaa atctatcaag tagacatgat gattcaaatg
    16321 atctgccaac aagatataaa aagtatgccg gatgatttat tggcacaaat tggtcaatta
    16381 agtactgaag aatggcgaag gtatcgaaaa taccagcaaa aaatacaaaa tgagcgcacg
    16441 aaaactgttt attttgatta tctgctacaa ggaaaacgca aggatcgttt gcagcatgaa
    16501 ttgcttaagc aaccagttga cacattactg atcctatatt tatattatct caaggaggaa
    16561 caaccatggc acaagaattt tcatatgaaa tcgtagaaga aatcgctata ttgtcagaaa
    16621 acaacaaggg gtggcgtaaa gaactgaacc tagtcagctg gaatggtcgc ccgccaaaat
    16681 tcgatcttcg agattgggcg ccagaccatg aaaaaatggg gaaaggcctg acattgacga
    16741 acgaagagtt tgaacaactg caaaaagcaa tagaaaatat gtaatgagga aggaccctgt
    16801 cgaatgaaaa gtatgactgg ttttggcaaa gccatccgcg aaacaaaagc gtatcagtta
    16861 gatattgaaa taaaaagtgt caaccagcgt tttctagatg ttcaaatacg aagtcctaaa
    16921 atcctgaact ttgttgaaaa cgattttcgt cagcttatta aacagcagtt aagccgcggt
    16981 cgaatcgaag tatttgtgaa tctagcttat attggcaaaa acgaaaaaca gatcgtcatc
    17041 aactgggaat tgattgattc acttatcact caattgacag acggaattca acaacgatac
    17101 ggtagtcaaa atcaagtgtc aattagtcgg atggcggaac gcttcgtgct ggacgaaaac
    17161 ttcgtaacgg tagaagaaaa accggaagag agcatggaag aattggcaca gttagctcta
    17221 gatacattga aaagcgcact tgattctatt gaggcgagtc gagaaaaaga aggtcaagct
    17281 ttagctttag tgttgaaaaa gaatactgaa gaattcaaag aagtactaaa aaaactcgat
    17341 agttttgtag aaatctatga aaaggaattt caaataaggt atcaaaaaaa gctggaggaa
    17401 tttcttggag cgaaagtcga tgagcaacgg ttgttgacgg agctagccat cttgttggaa
    17461 cgcggagata ttcacgagga actggatcgt ctggcgatcc atgtacaaaa actagatgaa
    17521 cttatcgaag cacaatgtcc tgtaggtcgg gaattagatt tcctgatcca agaaatgaat
    17581 cgggaaatca acacgatcgg ttccaaatca aatgcaatcg aaattaaaaa tcaagtcgtc
    17641 cagtctaaga cgatcttaga aaaaatcaga gaacaagtac aaaatattga atgacgaaag
    17701 aggtagagat aatgaaatat tatgacgtaa cgttccacga attaagtgga aaaactgtgg
    17761 ttaaacggga tatcccttct gaaaaaaacg gctttgacgt atggaaagat gcctgtgcag
    17821 actataatga gaacgaactg tttattttga tcaacgacgg tgcttacgtg acgatgaatc
    17881 ggaaatttat tgttcgaatt gacacagaag aggtagaaga tccgacagaa aaagcacgtt
    17941 ctcgaaaaga tgagatcatg ggtgtagtga atactttgtc caatatgggc ttttagcaag
    18001 gatgctgaac aaagaaatac aataaataaa cagaattgga tttcttcgct tagcttcttt
    18061 gctctaggaa tacatttaaa ggttgtgaca aaattcgtgt cacaatcttt ttttattttg
    18121 aataagtgat gttcaaaaac cattttctcg gtattaagcc aaatttatgc aaaaatgtga
    18181 gaaactattt tcacaaaatt tttcttcata ctcgaaacta atcggcttct tcacgacctc
    18241 ttatacacgg cgttcaaaag cttgcatctg cgataataac cccaaccaaa cgaaaaacat
    18301 gagaagctgt ttatgtttgg ctgttcttat tacttgatgc aggacagctt ttttacgacc
    18361 tcttggtaca gggtagcttt tttaccaatt tctagctata attaaagata accttgaaag
    18421 ttgcagaaaa aaagtgcaaa ataaacgata agtacaaaaa gactagttga aaggacgtcg
    18481 tcatgtcaga gcgtggatta ttaatcgtgt tgtctggtcc atccggtgta ggaaaaggca
    18541 cagtacgaaa agcaattttt gagagtgagg ataatgattt tcagtattcg atctcaatga
    18601 ctacccgtaa aatgcgtgaa ggggaggtcg aaggcgttga ttatttcttc cgaagcaaag
    18661 aagagtttga atcaatgatc gaagctggag agatgctgga atacgcagaa tatgtcggga
    18721 attattatgg aactcctttg tcatatgtca acaagacatt ggatgaagga aaagatgttt
    18781 tcttggagat tgaagtccaa ggagcaaaac aagtcaaaga aaaagtgccc gatggtgtat
    18841 tcatcttttt gactccgcca gatttagcgg aattaaaatc acggatcgta ggccgaggaa
    18901 cagatgcaga tgaagtaatc gaaaaacgaa tgaaagttgc aaaagaagaa atcgaaatga
    18961 tggctcttta cgactatgct gttgtaaatg atgaagtgcc gctagcagtt gaacgaatca
    19021 aaaacattat tgccagtgaa cattttcgag tagatcgcgt aatcggtaaa tatattaaaa
    19081 tgttaaagga gatgtagtct tatgatgtta aaaccttcaa ttgactcact attagatcgt
    19141 gtaaattcaa aatattctct agtgatttta gcaagtaaac gcgcgcatga attggatgca
    19201 agtgcacagc ctactctaga ttcttttgat tcagtaaaaa gcgtcggtca agctttagaa
    19261 gaaatcgatg caggaaatgt agtcaatgat ccacatcctg aattaaaacg tgaacgtttg
    19321 aagatggaag aagaggaacg tcaagcacaa aaagagcgtg aacaacacga attggaatca
    19381 cgtatccgtg aagagcaaaa aatgtaataa agaaaaatag agcgaacatc taaaaagaaa
    19441 agcaaaccca gctgtttcct tttcaaacat tcgccactta ttgtctggta gaaaaggctg
    19501 tgtctgaact ttttgacaca gttctttttt ttcttatctg ctgttaattt tttgttagga
    19561 tttggcagaa gataagacaa atccatcgat ctctagtaaa ataggcaagg aactttttgt
    19621 cgcttgataa gaaaagacaa aaaaaggagg aaacgaacca tgacattaac agctgaagtc
    19681 attgtggacg tacctactat gcagaccgat cagccattca cttatttggt tccctctgaa
    19741 atggaaacag ctttgcaagt cgggatgcga gtagaagtcc cttttggtaa tggcaatcgt
    19801 catgtccaag gatttgttat ggctatccaa aaatcagaag aatcagctaa tccatctttg
    19861 aaggcagtta ttcgtttgct agaccttgct ccggtggtca atgaagaatt actttcatta
    19921 gctgattata tgaaaaaaat tacttacgca ttcaaaatca cttgtctaca aacgatgctt
    19981 ccgagtgtga tgaaagcaga atatgataaa ttgatctatc ctttggcaga cacaccagaa
    20041 gttcaggtat tgtttgatca gcgagaagtg atttcctgga aggaagcaga agaagaaggc
    20101 agcctttctc aattgattcg ctggcgccaa gagcaattag tagatatcaa atatgaagtc
    20161 catacacgaa ataaagtaaa aacgattcgt ttggtccgtt ctttgttaac tgaaaagcaa
    20221 atcgaagaag aatgggcaaa attacggcaa aatgccaaga aacaaaaaga acttcttttg
    20281 tgtttatctg aactgagcca agaagaacca attgcttatt tcaaaaataa aggaatcagt
    20341 actgctgtct tgaatcaagg aaaagaaaag ggctggctgg agtttataga atcagaacgt
    20401 tatcgcgacc cttataaaga ccgagtattt gaccaaacga cggcattgga actgaatgca
    20461 gaacaaaaaa atgctgtcga acaaatcatc acagcaggtc agcagcaaaa agatcaggtc
    20521 tttttgctgg agggaatcac tggcagcggg aaaacagaag tatatcttca ggcaatcgct
    20581 gatgttttgt cagaaaataa aacggctatt atgttagtgc ctgaaatcgc attgactccg
    20641 caaatggtcg agcgattcaa aggacgcttc ggagaatcag ttgcggtctt acatagcgga
    20701 ctttcccaag gtgaaaaata tgatgaatgg cgcaagattg agcgggaaga agcgcaagta
    20761 gtagtaggcg cacgatctgc tatttttgcc ccgctgaaaa atatcgggct gatcattatc
    20821 gatgaagaac atgaatcaag ttacaaacaa gatgagacac cgcgttatca tgcgagagat
    20881 ctagcaattt ggcgcagtaa atatcatcat tgcccaatag ttctaggaag cgcgacgccc
    20941 tctttagaat caagagcacg agctcaaaaa ggcgtttatc agttattgca gttaaaccac
    21001 cgagccaaag cagctgcaca gcttccagct attgagatcg tagatatgcg tgaagagttt
    21061 cagaatcatc ggacctctac tttttctgct aatttgcagg aaaaaataca aaatcgatta
    21121 gacaaaaaag aacaaactgt cctactgctc aatcgtcgag gatattcgtc ttttgtgatg
    21181 tgtagggact gtggatttgt tcttccatgt cctaattgcg atatctctct tacactgcat
    21241 atggatacac gttcaatgcg ctgccattat tgcggacatg aagaacctat tcccaatcgc
    21301 tgtcctaatt gtggcggcaa taaaatccgc tactatggaa caggtactca aaaagtagaa
    21361 gaggaactcc gagaacttta tccgcaagct aggattttgc gtatggatgt agataccact
    21421 cgaagaaaag gggcacacga acagatttta caaaaattcg gtgcaaaaga agcagatata
    21481 ttattaggga cacaaatgat tgctaaaggt ttggactttc cagaagtgac attagttggg
    21541 gtgttgaatg ctgatacctc cctgaattta ccggattttc gttctagtga gcatacattc
    21601 caattgctta cccaagtttc aggacgagca gggcgagcgg aaaaagcagg cgaagtcgtg
    21661 atccaaacat tcaatcctca acattacgcc atagaattgg ctaaaaaaca aaattatgag
    21721 cagttttatc agcaggaaat gcatgtacgg catcgtggag gctatcctcc ttattacttt
    21781 actgtgaaaa taaccgccag ccatcctgaa gaacaagtag cggcaaagaa aatctttcag
    21841 attgccaatc agctaaaaga agtgttaagc cctcaaagct tgctattagg gccaacgccc
    21901 agcatgattt tgagagtgaa aaaccgatat tattatcaac taatcatcaa atataaacat
    21961 gaaccgaatc ttcctcatgt tttggatgaa attttgaatg gaagccagaa agaacagcga
    22021 caaggtcttt ttgtggcaat tgacaacgaa ccattaaact ttatttaaga aagggccaaa
    22081 ttatgcgcta tcctatattg atccacccta atgataaatt aaaaagaact gctcagccaa
    22141 tcgatgtaat cacggacgag acaatcgctt tattagacaa tttatatgag acaatgattg
    22201 cgaatgacgg aatagggatc gctgctcctc aagtaggaca aaacaagaga attgctgtga
    22261 tcgaagtaga tgaaggcgag aaatttgaac tgattaaccc agaaatcatc gaagcaaaag
    22321 gtgaaagcct tgacgtagaa ggttgtctca gtattccgca tgtctatgga acggtcaagc
    22381 gagcagatga agtgaccgtg cgttattatg atcgagacgg agaagagata gaagtgacgg
    22441 cttttggcta tttagcccgt gccttccagc atgaaatcga tcacttagac ggtatcttgt
    22501 tcattgagaa aatgatacag cagatcccgg aagaagaact tgaagaatat atggaggaac
    22561 atcttgatga ctaagctagt atttatgggc acacctgctt tttctgtgcc gattcttgaa
    22621 gggctgatag aatcaggtta cgagatccag gcagtggtaa cacagcccga ccgtccagtt
    22681 gggcggaaac gagtaattac gccgacaccg gtaaaaaaag cggctctgaa gcacggtatc
    22741 cgtgtgctgc agccagagaa aatctccggt tcgccagaaa tggaagaaat cattgaattg
    22801 gtgcctgatg tgatcgtcac tgcggcattt ggccaattct tgcctgaaaa gttattgcaa
    22861 gtaccaaaat taggggcaat caatgtccat gcttctttac ttcctaaata tcggggaggt
    22921 gcgcctgttc attatgccat catgaatggg gagaaagaaa ctggtgtaac gatcatggaa
    22981 atgatcaaaa aaatggatgc aggcggaatc tatgcgcaag aaagcatacc aatcactaaa
    23041 caagatgatg tagggacgat gtttgaaaag cttagccttt taggaagaaa gctattgcta
    23101 gaaacattgc ccaatatttt ggatggacaa aaaccagtac ctcaagacga gtcagaagca
    23161 actttttcgc caaacatcac acgggaacaa gaagcaatcg attggcataa aactgcagaa
    23221 gaaattgaca accaagtacg cggaatgcgt ccttggccaa ttgcattcac gacctatgag
    23281 ggcatacgct ggaaattatt atcggttgaa gtattggcag aaactacaga caaagcacct
    23341 ggtacgatca tccggaaaga caaaaaaaat ctatggattg cttgcggtaa acagaccgtc
    23401 ttagccatca atgaattaca gccagctggt aaaggaaaac aggccgtaca tgatttcctt
    23461 aacggaagcg gtcagcaagt tcagattggt cagcaggtga aataatgaaa aaaatcccta
    23521 aaaaaatgaa aaaatcagta cgatatatgg cattactgac attagagcga gtgaacaagg
    23581 gtggcgccta ctcgaatctt ttgttgaatg agatgatcaa acaaagcgag ctaaatcaaa
    23641 aagatgtggg tttatttaca gaattgatct atggtacgat tagccatcag cgactattgg
    23701 aattttacgt tgcaccattg atcgctaaag caaaaaaagt ggatgactgg gtcaagatgc
    23761 ttctttatct ttctgtatac caattagaat ttttagataa agtccctgca catgctattt
    23821 taaacgaggc ggtcgaaatc gcaaaagtaa aaggaaatcc tgggacaggg aaatttgtaa
    23881 atggtgtctt gaggaattat cagcgtcaag gagcaccaga tctgcaaggc attgctgatc
    23941 ctatcgaaag actttctgtc aaaatcagcc tgcctctatg gatgacgaaa cgactggtgg
    24001 atcaaatcgg atacgaagaa actgaaaagc tcggtttatc gctatatcag cctagccatg
    24061 ctagtgcccg agtagataca cgccgacttt ccagagatca ggccatcact gttttgcaag
    24121 aagaagagat cacagctgaa aaaagccaag tcagtccata cggtatagta gcgaaaaaag
    24181 ggcatttagc ttctagctca ttgttccacg atggcgtcat gacgatccaa gatgaaagtt
    24241 cgatgcttgt agcgccgtct atgcaaatcg agcctgaaca tcatgtctta gatgcctgtg
    24301 cagcacccgg aggtaagacg acgcatattg cttctttttt agatccgtca tgcggtggag
    24361 aagtggtcgc tttagatgta cacgaacaca aagtcaaact cattgaagat aatgcagcac
    24421 gtctaggtgt ctcttctgct gtctacgctc aaaagatgga tgctagagaa gtcagtaaac
    24481 agttcgaagc agagcagttt gatcggattt tagtagacgc gccttgttca ggtcttgggc
    24541 tgcttcggcg aaaaccagat attcgttata aaaaacagcc ggatgaatta gcgaatttac
    24601 caaaaatcca attggaaatt ttagaaagct gcgcaccaac tttaaagtct tccggtattc
    24661 tgacttatag cacttgtacg atcttaaaag aagaaaacca agacgttgtt tctgctttct
    24721 tggaaagaca ccctgatttt gagaggattg atgttttgac caatcctatg ctacaatcat
    24781 caatcgaaga gaaaatgttg actttgtatc cgcatcagtt ttacacagat ggatttttca
    24841 tttgttgttt gcgcaaaaag taatgaggtg aaaaaatgca gattgaatat caatcagatg
    24901 taggaagacg acgtaacaca aatcaagact atgccagtgt atttaccagc cgttcaggtc
    24961 tcaagcttgc tgtattagca gacgggatgg gtggtcatcg tgccggagac attgccagtc
    25021 agatggcggt aacgaatcta ggcgaagctt gggaaaaaca agaagctgtt gacgatgaaa
    25081 aaatcgccca atggttcatt caatcgattc aagaagaaaa tatgctgatt tatcagcgtg
    25141 gacaagaaca gccggaatat aacggtatgg gaacaacaat cgtagcggcc gctctgtcag
    25201 atgactggtt cacgattgcc aatgttgggg atagcagagc ttatttgatt cgtgaagata
    25261 aaatcatgca attgacggag gatcattctt tagtcaatga actagtcaaa tcaggagaaa
    25321 tcagcgaaga aatggcagtg aaccatccaa ggaaaaatat tttaacacgt tctgtcggca
    25381 tgcctggaac agtcgaagta gatgtttcta ctcatctttg gcaactaaaa gatcgactgc
    25441 tgttatgttc agatggcttg acgaacatgc tgtctgaaga agaaatagga acgatcatca
    25501 atcaagaagg cccattgtct cataaagtaa ctgagctgat tgatcgtgcc aatgaagcag
    25561 gcggagcgga taatatcacc gttcttctta tcgaatataa ggaggacagt gtatgatcga
    25621 gttgggcaaa aaattaaatg gccgttatca aatcactgga aatatcggta gcggtggtat
    25681 ggcaaatgta tttttggcgc acgatttaat cttggatcgt gatgtagcag ttaaagtctt
    25741 acgatttgac tttcaaaatg accagactgc gattcgacga tttcagagag aagctttagc
    25801 agcaacagaa ctcgttcatc caaacattgt tagtgtttac gatgttggtg aagaagacaa
    25861 catgcagtat ctggtgatgg aatacgtcaa aggaatggac ttgaaacgat atatccaaac
    25921 acattatcct gttccttatg aaacagcagt aaatattatg cagcagatcc tttctgctat
    25981 ttctttagct cataaccatc aaatcatcca ccgagatttg aaaccccaaa acgtactgat
    26041 cgataatgaa ggtgttgtaa aaatcacgga tttcgggatt gctatcgctt tatctgaaac
    26101 atcgatcacg cagacgaata cgatgctagg atctgttcat tatctgtccc ctgagcaggc
    26161 cagaggaagt atggcaacga agcagtcaga tatttatgct ttagggatca tcctttatga
    26221 gatgttgacg ggtagcgtac cgtttgatgg tgaatctgct gtaacaattg ctttaaaaca
    26281 ttttcaagat gatcttccat ctataaaagc acttgatcca aatgtgccac aagcactgga
    26341 aaacgtgatt ttacgggcaa cggctaaaga acctgcagac cgttataagt cagccgagga
    26401 aatgtctgat gatctttcta cagtattatc accagctcgt gcaaacgaag aaaaatggca
    26461 gcctcatgtg atggataatg aaacaaaagt tatcacaccc cttacggaag atgcaccaat
    26521 gcctgattca ttcaaatcta tgcctctgcc aaaagacaag ccagaaaacg aaaccttacc
    26581 agaggaggaa gaagttctgc ctgatcctaa aaagaaagga cagaaaaaat ggtggctgat
    26641 cctattagct attcttgctg ttttaggaat cggaacagct gtattctttg cttctggcgg
    26701 gcgaggagaa gttgttgttc ctgatgtttc tgatctttct gaagcttctg ctagagaaac
    26761 actcgcaaaa gcgaaattag aagtagcaac aaaaacagaa gaaattgcag atgacaaaat
    26821 cgaagaaggt aatgtagtga agacagaccc ggcagcggga acgacagtca agcaaaaacg
    26881 cgaagtcact ctgtatatta gtagcggcac gaaaaaaatc aaattagatg attacagtgg
    26941 taaaagttat gaagatgccg aagcaagttt gaaagaatta gggttttctg ctgacttgat
    27001 tcaaaaagaa gaggaattca gttcagaggt agaaagcgga atgatcattt cgcaatctcc
    27061 atcagaagat acagaagtcg atcctaaaac agacacgatt actttcacag tcagcaaagg
    27121 accagaaaca gtgactttga gtagtcatgt aggagaaaac tacgaaactg ttcgtaattg
    27181 gcttgttaca caaggattca atatcaataa tattagtgct acttatgact atagcgacac
    27241 tgtaccatca ggttcgatca tcagtcagga tccagcctcg ggtgaagttg tagcagaaga
    27301 aacttacatc aattttgtcg taagccaagg aacagagcct aaattgaatg atatctccgg
    27361 ctatacaaaa agcgaagcac aaagttattt agctagtgtc ggtgcagaat atatcgggca
    27421 tgagacttcc gaatattcaa acacggtgga taaagataaa gtgattcgta cagtaccagg
    27481 ggcaggaacg acaattacca aaggaatggt tgtgaacgta atttactcta aaggaccaga
    27541 tccagcgtct tctagttctg aaaaagactc ttcagacagt tctagcagct caagtagctc
    27601 cagtagcaca agcaactctt caaaagaaga atcttccagc tccacaagtt catcagctag
    27661 cacctcagaa agttcaaaat aagtgctgaa taaaatatat cagaagggaa tgcgacattg
    27721 atttgttgca ttctcttttt tgtgtttcta tctcttttga tcaattttca agccatatac
    27781 tttgagaagt ttgcatggaa taacacctac tttcctcttc gtatggtaaa ataaacaaaa
    27841 acggaggtgg cacttatcaa aggacaaatc agaaaagcaa tcagcggttt ttactatgta
    27901 tacgcaaacg gagagaccta tcaaacaaga ggaagaggaa atttccggaa tcggaaaatc
    27961 acgccgcttg tcggagatca ggtgatcttt gagagtgaaa atcaaacaga cggctatctt
    28021 ctggaaatct tgccgagaaa aaatcaatta gttcgcccac cagtcacaaa tgttgatctt
    28081 ggaatcattg tcacaagtat cgtagagcct gcattttctt ataatctact tgaccgcttt
    28141 ctagttacct tggaatacga gtcgatcgaa ccgattattt ttttgacaaa gaccgacttg
    28201 tcagacgacc cagaaaaaat cgaagagatc aagcaaacct atactaaaat aggctatccg
    28261 gtgattgtac ccaatcatac gggtgatacg acagaactga tccgttactt cccagaacgg
    28321 ctatcggtct ttatgggtca gtctggagca ggaaaatcga cgctactgaa tcaaatatca
    28381 ccggaactga atttagcaac aggagagatt tctgatgcac ttggaagagg acggcataca
    28441 acacgtcatg tggaactgat tcctatctat ggcgggttag tagcagatac accaggattt
    28501 agctcgattg actttttgac aattgaagca ggagaactgc cgagacaatt tcctgaattt
    28561 gtagaagctt ctgtccattg caaatttaga gagtgcatgc atgctaaaga acctggctgc
    28621 gaagtaaaac atagagtaga aacaggtggt atcgcacaga cacgttacga caattatttg
    28681 caattcttac aagaaatcga aaaaagaaaa ccaatgtatg caaaaaaaga aggtaaacga
    28741 aaataggagg aaacaagaat gaagatagca ccatccattt tgagtgcaga ttttgccaat
    28801 ttacaaagag acattgagtt agtggaaaaa ggaggcgcag attacatcca cgtagatgta
    28861 atggatggcc gatttgtccc aaatatcaca ttagggccaa atattgttca ggcaatccgt
    28921 cctgttacta aattaccgtt agacgtccat ctgatgatcg ttgatccaga aaactacatt
    28981 ccagcatttg caaaagctgg agcagatatc attactgtcc acgtcgaagc aactcctcat
    29041 attcatcggg cacttcaaat gacaaaaagc ttaggagtta aagctggagt agttatcaat
    29101 ccaggaacac cagtctccat gatcaaacat gttttgcctt tggctgatca agtactagtc
    29161 atgactgtca atcctgggtt cggcggacaa agctttattg aagaaacagt agaaaaaatt
    29221 acggaactcg ctgatctacg tagagagaac aattggactt atgcaatcga agtagatggc
    29281 ggaatcgttc cagaaacagc aaaaatctgt aaagatgcag gggcggatgt cttcgttgca
    29341 ggatcatata tctatggagc cgaaaatcca gtcggacaaa tcgaacgatt aaaagaggca
    29401 ttagactaaa tgaaaatttt attagctgct ggaggtaatc cgcaaaactg gccgcagtct
    29461 ctgctgtcca aatacgatgc ctatgtcggg atagaccgtg gtgcgctgta cctgcttcaa
    29521 gcaggctacc cagtagatct ggcagtgggt gattttgatt cattgtcgaa cgaagagcag
    29581 aaggaagtat ttgcaaaagc caaagagtat acccgttccc ctgcagaaaa agacgataca
    29641 gatacacagt taggtatctt aaaaacattt gaacgttacc cgcaagcgga agtcactttg
    29701 attggagcta cgggcggaag attggatcat cttttagcca atctctggtt aggtatggaa
    29761 cctcgcttca aaccctttgc ccatcagctt attatcagag attgtcaaaa ttatttgtct
    29821 tattatttgc ctggagagca tatagtaaga cagataccag atatgaagta tttagcttat
    29881 tgctgcttga ctcccgtctc gaatcttaca ttaaaaggca gcaagtatgt attggaaaat
    29941 aaagaagtag aatatccgac atcttatgca agtaacgaat ttctttcaga taccgcagaa
    30001 ttttcttttt cagatggtat gattgcagtt attcaaagca aagattgatc cataaacaaa
    30061 taaaaaaatt tgtctttcag gctaaaaatg acagaatcta ttatttgcta aaaaaaagct
    30121 taagaggagc aacttacctt ttgtttcacg gcatttagtg ataaaattga acacaagagt
    30181 ctaattaatt cgaggtgtct ttgtgaagaa gttcaatcct aataaaaata ttattattac
    30241 cttggttatt gttatcatcg tggtgacgat actaagtgtg accgctgcgc agcgagcgaa
    30301 tgaaggaaag cccaatttct tccaatccat cgttaatgac agtgttgcaa tggttgataa
    30361 aacaatctct gccccagtac ggtgggtacg aaatggagta gattctgtcc ataatctttt
    30421 ttcaacttat gccgaaaatg agcggctaaa agaaaaaatc gatagctatg atgacgtttc
    30481 acagcaaaac aagaattacg agagagaaat cgaagcgttg aaaaaagaac ttgagttgaa
    30541 tcagacgctg actagttacg aaaaagtaac ggcaaatgtc attactcgtt cgcctgacag
    30601 ttggcaggac atgctgatcg tagacaaggg ttctaaagac ggtatcgaag caaatatggc
    30661 agtcatgtcg caaaaaggat tgattggccg agtgatcgag gtcaatacgg cttcgtctaa
    30721 aatcgaatta ctgtcatcct ctaatgaaag ctccaatcat tttccagtac gggtatcttc
    30781 ggctaatggc gaagcgtttg gtttgcttaa aaactatgat gaaaagctcc atgccttagt
    30841 ggtgacccaa ttaactggtg atacggatat caaagaaggg gatgttgtcc agacatccgg
    30901 tcttggaggg aattctccag ctaacttgcc gatcggtacg gttattaaaa cgaaaccaga
    30961 tagttatggg ctggatcggg aagtttatgt gaaaccttat gcagaaatgt atgacgtgtc
    31021 agttgtgacg attgtccaac ggctagcgga ggattagtcc atgttgaaga aagaaacaat
    31081 gaagtactat ctgccaatcg ttttgttctt tttgatgttg atagatggtc atttaacaag
    31141 aatgctaggg gagtggtcga aaggcaccta tatgtcaaat gcccactttc tgatattggc
    31201 attattatgt tgcagtatgg cgtttgaaaa acgttattta ctgattacca cgattgttct
    31261 cggggctatc tatgacgctt actatattgg cgttatcggt atctatgcag tagctctccc
    31321 tttaattgta tggttgatgt atgtaatgaa agacgttatc catgtcaaca tctttactga
    31381 atttttcagt atgatcatct ttgtcacggg ttatgaattg tttacgatgg tggtccagtt
    31441 gatttttaaa ttagcagtag taaataacac gtattttatt acaaggtttt taggacctac
    31501 actgctgttg aacatgatta tatttgtatt attcattttt ccctttaaga aattattcag
    31561 tgatgaataa tttctttttt tatttttagg tcgaaagcga aaaagaatcg ttgttatctt
    31621 aagaaatgct agacttaacg cggctttttc atgatcattt tctgaaacga acagttttgt
    31681 catttttaaa agttgttttg aaacattttt gtaacataac tattatatcg ttgacacact
    31741 gctatggtac aatttttata tcgttaagaa agacttaaat atatttagat atttattaaa
    31801 ttaagccagt cggaggaatg aagagtgaaa aagagtttaa tatcagcagt aatggtatgt
    31861 tcaatgacct tgactgccgt agcatcaccg atcgctgccg cggcggacga ttttgattct
    31921 cagatacaac agcaagatca aaagattgca gacttgaaaa atcaacaagc agatgcacaa
    31981 tcacaaatcg atgccttaga aagccaagtt tctgaaatca acacacaagc acaagaccta
    32041 ttagcaaaac aagatacatt gcgtcaagaa tctgcacaat tagtaaaaga cattgcagat
    32101 ttacaagaac gtattgaaaa acgtgaagat acaatccaaa aacaagcacg cgaagcacaa
    32161 gtaagcaaca caagctcaaa ctacatcgat gctgtattga atgctgattc attagcagat
    32221 gctattggac gcgttcaagc aatgacaacg atggtaaaag caaacaacga cttgatggaa
    32281 caacaaaaac aagataaaaa agctgtagaa gacaaaaaag ctgaaaacga tgctaaacta
    32341 aaagaattag ctgaaaacca agctgctttg gaatctcaaa aaggcgatct tttatctaaa
    32401 caagctgatt tgaacgtact gaaaacttca ttagctgctg aacaagcaac tgctgaagac
    32461 aagaaagctg acttgaaccg tcaaaaagca gaagctgaag cagagcaagc tcgtattcgt
    32521 gaacaacaac gtctagcaga acaagctcgt caacaagctg cacaagaaaa agctgaaaaa
    32581 gaagcacgcg aacaagcaga ggctgaagca caagctactc aagcttcatc tactgcacaa
    32641 agctcagcaa cagaagaatc atcagctaca caatcttcaa tgactgaaga atcatcatca
    32701 gctacacaat cttcagcaac agaagaatca acaacacctg aatcttcaac agaagaatca
    32761 acagcgcctg aatcttcagc aacagaagaa tcaacaacag cgcctgaatc ttcagcaaca
    32821 gaagaatcaa caacagtgcc tgaatcttca gcaacagaag aatcaacaac agtgcctgaa
    32881 tcttcaacaa cagaagaatc aacaacacct gcgcctacaa caccatcaac agatcaaagt
    32941 gttgatacag gaaacggcac aggaagtagt actccggctc caacgccaac accaacacct
    33001 gaacaaccaa aacctgtaac acctgctcca gctccttctg gaagtgtgaa tggtgcagca
    33061 atcgtagcag aagcttacaa atatattggt actccatatg tttggggcgg taaagatcca
    33121 agtggctttg actgctcagg gttcacacgc tatgtttaca tgcaagtaac tggtcgtgac
    33181 atcggtggtt ggacagttcc tcaagaatca gctggaacaa aaatttcagt ttctcaagca
    33241 aaagctggag acttgttatt ctgggggtca caaggcggaa cttaccacgt agcgattgct
    33301 ttaggcggag gacaatatat ccatgctcct caaccaggcg aaagcgtaaa agttggttca
    33361 gttcaatggt ttgcccctga ctttgctgtc agcatgtaag aatga
//
LOCUS       DAYYAG010000017        32800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000017 DAYYAG010000000
VERSION     DAYYAG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.017"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..1474
                     /locus_tag="ACHARQ_000708"
     CDS             200..1474
                     /locus_tag="ACHARQ_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM8409158.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1857..2585)
                     /locus_tag="ACHARQ_000709"
     CDS             complement(1857..2585)
                     /locus_tag="ACHARQ_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409159.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2563..4137)
                     /locus_tag="ACHARQ_000710"
     CDS             complement(2563..4137)
                     /locus_tag="ACHARQ_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8409160.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4366..5304
                     /locus_tag="ACHARQ_000711"
     CDS             4366..5304
                     /locus_tag="ACHARQ_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409161.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5316..6227
                     /locus_tag="ACHARQ_000712"
     CDS             5316..6227
                     /locus_tag="ACHARQ_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409162.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            6251..7723
                     /locus_tag="ACHARQ_000713"
     CDS             6251..7723
                     /locus_tag="ACHARQ_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8409163.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7774..8385
                     /locus_tag="ACHARQ_000714"
     CDS             7774..8385
                     /locus_tag="ACHARQ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8409164.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8415..10328
                     /locus_tag="ACHARQ_000715"
     CDS             8415..10328
                     /locus_tag="ACHARQ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8409165.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10456..13437
                     /locus_tag="ACHARQ_000716"
     CDS             10456..13437
                     /locus_tag="ACHARQ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8409166.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13434..15095
                     /locus_tag="ACHARQ_000717"
     CDS             13434..15095
                     /locus_tag="ACHARQ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8409167.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            15135..16067
                     /locus_tag="ACHARQ_000718"
     CDS             15135..16067
                     /locus_tag="ACHARQ_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8409168.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(16459..18006)
                     /locus_tag="ACHARQ_000719"
     CDS             complement(16459..18006)
                     /locus_tag="ACHARQ_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8409169.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(18108..18461)
                     /gene="tnpB"
                     /locus_tag="ACHARQ_000720"
     CDS             complement(18108..18461)
                     /gene="tnpB"
                     /locus_tag="ACHARQ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8409170.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(18451..18645)
                     /locus_tag="ACHARQ_000721"
     CDS             complement(18451..18645)
                     /locus_tag="ACHARQ_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409171.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(18871..20073)
                     /locus_tag="ACHARQ_000722"
     CDS             complement(18871..20073)
                     /locus_tag="ACHARQ_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8409172.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(20076..20270)
                     /locus_tag="ACHARQ_000723"
     CDS             complement(20076..20270)
                     /locus_tag="ACHARQ_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8409173.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(20317..21165)
                     /locus_tag="ACHARQ_000724"
     CDS             complement(20317..21165)
                     /locus_tag="ACHARQ_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8409174.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(21370..21720)
                     /locus_tag="ACHARQ_000725"
     CDS             complement(21370..21720)
                     /locus_tag="ACHARQ_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409175.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            21898..22761
                     /locus_tag="ACHARQ_000726"
     CDS             21898..22761
                     /locus_tag="ACHARQ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8409176.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            23385..24377
                     /locus_tag="ACHARQ_000727"
     CDS             23385..24377
                     /locus_tag="ACHARQ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409177.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            24393..25277
                     /locus_tag="ACHARQ_000728"
     CDS             24393..25277
                     /locus_tag="ACHARQ_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409178.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            25326..26933
                     /locus_tag="ACHARQ_000729"
     CDS             25326..26933
                     /locus_tag="ACHARQ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8409179.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            26934..28418
                     /locus_tag="ACHARQ_000730"
     CDS             26934..28418
                     /locus_tag="ACHARQ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8409180.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            28595..29587
                     /locus_tag="ACHARQ_000731"
     CDS             28595..29587
                     /locus_tag="ACHARQ_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8409181.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            29639..30508
                     /locus_tag="ACHARQ_000732"
     CDS             29639..30508
                     /locus_tag="ACHARQ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8409182.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(30928..32541)
                     /gene="guaA"
                     /locus_tag="ACHARQ_000733"
     CDS             complement(30928..32541)
                     /gene="guaA"
                     /locus_tag="ACHARQ_000733"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8409183.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
     gene            complement(32548..>32800)
                     /locus_tag="ACHARQ_000734"
     CDS             complement(32548..>32800)
                     /locus_tag="ACHARQ_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8409184.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT        10280 a         5523 c         6605 g        10392 t
ORIGIN      
        1 gctctttgtc aaataggact gatagagtga tataagaaca tctaaggtag aattgatttt
       61 gcttctataa tattttgtgt tggtggaaaa tcgaaggcga ttttctacta acactttttc
      121 ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacgtcatc
      181 ctaccaaagg agtctctcaa tggataatca tactagaaaa ttactcggat taacagataa
      241 acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca
      301 gtttattaaa ggaaaattgg atgactctcc ttcctattgt gaacactgtc gatcggttcg
      361 tattatccgc aacggttcct atacgacacg cactcagata ttgaaagtca aagaaaagtt
      421 aactatttta gaattaaaac gtacccgttt tctctgccat gattgtggaa ggaccttttc
      481 tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcaat
      541 tctcatggaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc
      601 ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagt cacgtatgaa
      661 cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg tttctggttc
      721 catgagtttt atttgtgttg atgggacaac ccatcagcta tttacgatcc ttgaagatcg
      781 cagactttac aaactaactc aatacttcct gcactttcct agaaaagccc gtttaaaggt
      841 caagtaccta gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa cagtctttcc
      901 aaatgcggag atcgtaactg accgctttca tattgtccaa cacatcaatc gcagcttcaa
      961 tcaattacgt gtccagatca tgaatcgttt tcgaacctca cactctgaaa atcagaagaa
     1021 gtaccgtcgg ctcaagcgat attggaaact cttactcaaa gattccacga catgggagcc
     1081 actcaaacgg cattatcatc gactgtttaa acgaccaatt agtcaaacag aaatcgttga
     1141 tgagctgtta agctacaacg aagaattgcg aactgcgtat catttttgtc aattattacg
     1201 ctattatttc gttaaacgag gtacggaagg ctttcaagaa acccttaaaa caatttcatc
     1261 tacgttacct cagtcgttta aaaagaaatt cacgatcttc cattgctacc aatcgggtat
     1321 ttcaaatgcc tttaaggtgt cccactcaaa cggcgtcact gaggggctga ataacaagat
     1381 caagctgatt aaacgaattg cgtttggtta tcgcaacttt tataactttc gggcacggat
     1441 ctatttacaa caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca cagaacgcgc
     1501 cgcatatcgg actaagtttt attcaaattt gtcttcacca acacgatttg acgaagagcc
     1561 tatttaaata actacatcca tttattctat cttctaaaat agcctttttc atttcagtta
     1621 aatagttcag cgccattttt gcactcgact tgcatagtag ttttttccat attcctcaac
     1681 ctcagcgggg gttgaagaca taaggaaaat cctatgcgtt atgctcaact ggatctgatt
     1741 aaatattcta atgtattttt ctctaccatc attaaatggt taatagccaa aaatcaccac
     1801 ttttcaatgg ctccttgttg tataagtata actagaagat gaacacgtct gagaaattat
     1861 agaagctttt tacggtaatt actggggctg tgtccagtag tttttttgaa gagaacagaa
     1921 aaatagctca cgtgatctat tcctactttt tgactaattt catcaataga taagaatgtg
     1981 tctttcagta gagcacttgc tcttttgatt cgaaagtcta gcaagtaatc atgaaaggaa
     2041 taacctgttt cttttttgaa ggcccgtcct aaataagcgg gatggtaatg gaattgtgta
     2101 gctacatctt tgagcgtaag cttttcacta taattgtttt gaacgtaatc aatcacttgt
     2161 tgaattgact cattaaaaga atcatcagtt agagagtttt gattagctgc tgatttacgc
     2221 attttttctt cttgtactaa cttaactaaa taaggaaaat caatgggttt cgttatgtaa
     2281 cttttaatag ataaatcaat cccctttttt gcatactgaa aattttggtg ggcactaatg
     2341 acaatactag taaagtagcg attggatgtt tgttgcaaag attcgattaa ttcgagtcca
     2401 gaaattttcc ccatcgtaat atctgtgata agtaaatcaa agcgctggtt ttgcaggttt
     2461 ttaaatgctt cgtctccgtc tgtgtagcag tgatcgataa tgatgtcggg gactttcttc
     2521 aaaagataat gccttatgcc ttctacaatt gtacaatcat cgtcaacaat tgcaattctc
     2581 aacatgcata ttcccccctt tttgtatttt gatagtaatc gttgttccga cgtgtagttg
     2641 gctttctata acaaaacgtg tatctccagg atagagatgc tttatacgtc gaaaagaatt
     2701 tgagatgccg atattctcac tataaggaat atcatcatta agtttttgtt gaatatgctc
     2761 cagcttgtct tggtccattc ctttcccatt ttcaacgatt tttaacacat aatggttggt
     2821 tgtttcagtt gcatagacag tcaaacgatt ttcctttttt gatgaatcga agccgtgttt
     2881 aatataattt tcaacaaatg gctgtaaaga aagttttggt aacattaatt gatcaatatg
     2941 atcaatcagc tgatagtcaa agttatgtcg ataacgacgt tgatgtagtt gcaaggagag
     3001 gtcgatatta attaattcct ctttcaaatc aattatgtcg ggctgatatg tattattgcg
     3061 atacaacttg ctcaataaat atatactgtc gctgatttct tcgtcatcat tcaatatggc
     3121 agtcattcga ataaattcca aattattaaa tagaaaatga ggctcgattt gatattgaag
     3181 tgctgcatac tttgcttctt gcttcaacca attattttca taaagtgttt tttgatatgt
     3241 agcattatct aaataaaact gattgatatg catagcgatt tctttcaaat caccgacttt
     3301 gttttcgata ttgattgact tgtagtctcc tgtagatgaa tgatttaagg aagtaatgat
     3361 ttcctgatac tgctggatat agttacgaaa acttctattt aataattgat gaatagcaag
     3421 aataaacaaa ctcattaata agaaaatcat gattgccaga cctaataagg tttgtgacga
     3481 ataatttgga ttttcatata cttgtaatgt gtggttaccg atagctatct gttgcgtttt
     3541 tttccattta gaaggcgaga tgtcttcaga taaattgacg gaatcaatac cattagacaa
     3601 agaataattg taatgtgttg gaaagagact cttttttagc gcgttgatcg aaacgtctag
     3661 aatcaatgta gcatcgggca tgtcctcact aatgaaactt aatgaacgaa taaaagaaat
     3721 tttttcgttt tttgtaggtg cgtaggcact taagtatttt tcgtaatcta atgagataga
     3781 tatattttta acttcatcat cttgcacaag tttttttaag ttatctacta aataggtagg
     3841 ggagcgatcc tttgacaaaa tatcttccat gtattcagca ggtgatgaga ggtagtagtc
     3901 attaaggttt tcttggaaat cataattttg atagatctct gtcacgatac cggagagaat
     3961 acgattatat gtttccatat agtgggtgat ttgctgaatg ttttgcaggt tttgattttt
     4021 ttctttttca atcacattga tataggaaaa gaacaatgtg aaacaggcaa gtaaagtaat
     4081 aaaaaaaatc aatatgtatg taaaacgcat aataactttt gataaatcgt ttttcattca
     4141 aagtcctcat tttgataatc tctaatttta tgaatcatta tagcacatat tacaagtgat
     4201 gtaaacgctt tgcaaacagg gctacttatg attgtgaata cagtttccgt ttaaaaacta
     4261 ttctattgta gaggtttaat aaactaaaaa taatgtataa aaaattaatg taagcgatta
     4321 accaagtggg tataatcaag gaaacgctga gagaggagag acaaaatgaa gctaaagaaa
     4381 gggttattag gaaatctaaa aaaaaaccga gcattgttta ttatgctttt accaggtgcg
     4441 ttatggttat ttatattttg ctatatacca atgtttggag ttgtaatcgc ttttaaagat
     4501 ttcagaattt catcaagggg attctttaca agtttgttca acagtccttg ggttggctgg
     4561 gaaaatttca aatatctctt tgcttcgcaa gatgcttgga tcattactcg caatacggtt
     4621 gtttataacg ttgtaggtat tttgctaagt actttttgta cagtgtcatt agcaatcatt
     4681 ctgagccaaa tggcgaacaa acggttattg aaaaagatgc agacatttat gatttttcct
     4741 aattttttat catgggtagt catcagttac tttgtttacg catttttgag tacggataaa
     4801 ggattaatta atattttact aagcaatttt ggtgtggaac ccataaattg gtatagtgaa
     4861 ccgaagtact ggccttttat tattgttttt acgagtttat ggaaaggcgt aggctatgga
     4921 tccatcattt attacgcgtc aattatgggg atcgatacta gttattatga agctgcttta
     4981 gttgatggtg ccagtaaatg gcaacagata aggaatgtag tcttgccaca tttaagatcg
     5041 attattgtca ttatgttgat tttgtctgtt ggtagtattt ttcgagctga ttttggattg
     5101 ttttatcaat tgccaagaaa ttcgggggct ctttatgatg tgacgaatgt attagatact
     5161 tatgtctacc gtggattaat gaatagcgga gatatcggaa tgtctacggc agcgggatta
     5221 tatcagtcat cagttggctt tgtcttgatt ttgattacta ataagatagt caacaagtta
     5281 gatcctagtt cagcactatt ttaggaggaa tcaaaatgat atcaaaatta aaacggcaga
     5341 tgtacttttc gctgccagtc gaacggtttt tcacaatatt actaatctta tttactctta
     5401 gctgtatttt tccctttatt ttcgtaatcg tcatttcatt aacagatgaa aaagcattac
     5461 aactcaacgg ttacgaatta ttgccggctc aatggagcac tgatgcatat caatacttaa
     5521 tacaagatgg cggacagtta cttcggtcat taggcgttac aattatgatt acagtgattg
     5581 gaacgttgat tactgttttt atgacgggaa cctatgctta tgttttatca agagcctctt
     5641 tcccttaccg gaagttcttt actttttatc ttttttttac catgttattt gcgggaggga
     5701 tggttccttc ttacttagtg atgacgaaga tgcttggctt aaaaaataca atttgggctt
     5761 tgattctacc tcttgctttt agcccataca atgtcattat tttaaggaca ttctttaaga
     5821 agtcgattcc agaatcgatt atcgaatccg ctaaaatgga tggctgtagt gagtttcgag
     5881 tttttttcca aatcgttttg cctttagcga tcccaggggt tgcaacaata ggtttatttt
     5941 caagcttagg ttattggaat gactggttca acgcattgct gtatattgac accaacaaat
     6001 tgattccatt gcaatatttg ttaatgaaga ttcaaaattc aatggaattc ttagcaaata
     6061 ataatgacat tacattagcg cagcaacaag caattcaaaa tagccttcct caagaatcaa
     6121 cacgaatggc aatggtcgta gtagctactt taccgattgc aattgtttat ccgttcttcc
     6181 aaaaatactt tgttcaagga ttgacgatcg gtggagtgaa agaataaaaa agaataggag
     6241 atgttaaaag atgaaaaaga acatgcagaa atatttgatt ggtggtgctt ctgtactaac
     6301 ccttggccta ttaggtgctt gtggtagcag taatggcgaa gcaaataatg aaacaggaac
     6361 cacaaaagtt acttggtatc aggttggaga agaacccaaa gattttgatg aggtcatgac
     6421 aaaagtcaac gaaaaattaa aggaagaaat caatgtagag ttggatatgc ggctgattcc
     6481 ggacggggat tatcagcaaa agcttggtgt gatgatcaat tcaggagaag aatatgatat
     6541 ttgtttcgtc aatggcacag attatgtgaa ttatgggaac aaaggtgctt tcattagtct
     6601 caacgatcat ttggataaag agttgaaaga ttatgcagct gaattgaatg aaggatttat
     6661 tgaaggtggg gcaattaatg gcgaaactta tggattgcca gtcaataacg atattgccac
     6721 aacggaatat tggaccttta ataaagattt ggtagatcaa taccaattgg atgtagagaa
     6781 tgttgaaggt gtagaaacat tggctccagt attagaaaag tttgcagaaa aaaatactga
     6841 tacgaatatt acgccaatcg gtatgacccg taccagtgtc tgtattcctt atgacaatat
     6901 tatcggtggt ggtgtaccgc tagcggttgc atttgaaggc gatacacaaa cagttgttaa
     6961 tacttatgag actccagaaa tgatgactta tttgaaggct atgcatgagt tttataaaaa
     7021 aggatatatt ttgaaagatg cagccactgt tacgacagat ccatttcgaa ttgatagtaa
     7081 aaactggttt ttaggtcgta actcaagtgg ttatcaacca ttagcacaaa aaaaattaga
     7141 agctctagcg ggacatggat taattttaaa accagctgga aaagctttta aaacgactgg
     7201 atttgcaaga gtggcaatgc atgcggtatc tgcttcaagt aaacatccgg aagcagcctt
     7261 gaaattgttg aattatgtga ataccaatca agaagtagcg gatttaatgg tttatggaat
     7321 tgaaggaaaa cactatgatc gcaatgaaga tggttcgatt actttaacaa actctgatga
     7381 ttattatcaa ggcgcttggg cattggtaaa tacttatcta gtttcaaaaa cttcaccgat
     7441 tacagaagaa agtaaaaaac tagaagaaga aagtaaaaag tttcaaaatg aagcaccgat
     7501 gtctcctatt ttaggtttct cttttgacgc ttctgatttt cgaacagaaa ttgcaagtgt
     7561 acaaaatatc gtgtcacaat atgaagtact tttaagctcg ggaacagttg accctgaaca
     7621 atatgtacca gagttgttag aaaaactaga aaaagctgga ttggataaaa ttcaggcatc
     7681 tgctcaagaa caatacgatg aatggcgaaa atctaacaaa taatttttta ctaaatgaaa
     7741 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     7801 tttaattggg tttttcaatt gttgttacta aactttactt attttatact aatttgttta
     7861 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     7921 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     7981 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     8041 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     8101 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     8161 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     8221 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     8281 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     8341 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     8401 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     8461 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     8521 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atttgcttgt
     8581 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     8641 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     8701 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     8761 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     8821 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     8881 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     8941 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     9001 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     9061 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     9121 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     9181 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     9241 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     9301 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     9361 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     9421 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     9481 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     9541 tgttaactac ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg
     9601 cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct
     9661 acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg
     9721 aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt
     9781 ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca
     9841 ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt
     9901 ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa
     9961 ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc
    10021 ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat
    10081 tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat
    10141 tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa
    10201 atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc
    10261 aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct
    10321 acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg
    10381 ttcgttttct attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt
    10441 aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat
    10501 acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag
    10561 ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat
    10621 tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagctat
    10681 gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc
    10741 aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt
    10801 gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa
    10861 ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg
    10921 agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt
    10981 caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga
    11041 atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt
    11101 tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt
    11161 caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat
    11221 ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa
    11281 gattgccttt tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat
    11341 ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta
    11401 ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa
    11461 ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta
    11521 aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg
    11581 gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat
    11641 ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg
    11701 attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg
    11761 tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca
    11821 cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac
    11881 caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat
    11941 gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc
    12001 cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat
    12061 gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact
    12121 ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat
    12181 ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac
    12241 cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc
    12301 ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat
    12361 atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa
    12421 agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt
    12481 tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa
    12541 gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa
    12601 ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct
    12661 gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca
    12721 atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga
    12781 tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc
    12841 ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac
    12901 atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat
    12961 ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg
    13021 aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg
    13081 atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa
    13141 gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc
    13201 atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc
    13261 gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat
    13321 aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt
    13381 atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag
    13441 aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa
    13501 accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc
    13561 gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac
    13621 agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc
    13681 atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat
    13741 cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg
    13801 taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc
    13861 gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt
    13921 tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat
    13981 tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc
    14041 ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg
    14101 atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga
    14161 ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag
    14221 gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag
    14281 tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat
    14341 atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat
    14401 taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc
    14461 aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg
    14521 attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc
    14581 tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag
    14641 cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag cgttttgatc
    14701 ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt
    14761 atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag
    14821 aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag
    14881 cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt
    14941 ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc
    15001 caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag
    15061 taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga
    15121 aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa
    15181 tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg
    15241 aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag
    15301 catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg
    15361 gactaccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga
    15421 ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc
    15481 ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg
    15541 aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag
    15601 gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt
    15661 cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata
    15721 aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag
    15781 ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat
    15841 atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat
    15901 tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg
    15961 ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag
    16021 gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa
    16081 aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc
    16141 actttaaaaa agggactgtg acagaagtga aaatagacaa aaaatatccg aacaagagac
    16201 taggatagca gaaaaactat cgttttgcaa cgaggaccaa ctggaccgta ggagcaattc
    16261 tcttattgtt cggatatttc gcttgttcaa ggacttatgt ctgtagtgcc cttttttggt
    16321 aggaaaaatg gtagggaacc atatcatttt taatgaaaat caagcatttc aaaagttgaa
    16381 aagtaagcgc cccatagaac agtacctgta cgtcaaataa cccgacctat agaatagaat
    16441 cgggaatgtt tggcgttttt agttacgaga aaaatgttgt tgtacatcct tgttccaagg
    16501 caacagttga tctaaagttt ctgctgaaag tacgggaaga tttggtatct tatcaaatag
    16561 aaactctaga tactttacag gattcaatcc gttaagttct gctgttttat atacactcag
    16621 aatgattcct gaagttcgtg ctccttcaag acttgtggaa tgcaaccaat ttttccgtcc
    16681 tatcaccagc tctttgatat ttcgctccgc acgattattt gtgagttcta aacgaccatc
    16741 ttcaaaaata ggttcaaaac cacttcgtag tttacacgca tattgaacgg ctgcatccaa
    16801 cttggatttt gcaacaggat ctatcgttgc catccactca taaaaagcaa taaataatgg
    16861 acgcagttct tgttgccgac agcttaatcg cttctcagga gaaaagtctt tccatttctt
    16921 ttctaggaca aaccatttat ccaattgttt gactgcttga gctgcgggaa tatccgtgtt
    16981 tttctttcct tttggaattg cttcgaaaaa tttccgccga acatgcgcga aacaatagac
    17041 aagtcgtaca ctttccacgg cattataccc gctatagccg tcacagtgta aataccctgt
    17101 aaactccttg agaaaagttt tcggaacatc tccagcacga ctttcgtcgt ggtgataata
    17161 aacgatggga tgctgactgt cttttcctgt acaaaactgc cagtaatagg tatctgtttt
    17221 ggcactttca atcacacgat aacttgtttc atccccgtgt agaatctctt cttgattaag
    17281 atagactctt aatcgttctc ctaaagggtc taacgcgttt tgcactgctt taatatgcca
    17341 attggtgata ttatctctcg aaatatccaa cccatgtgtt tgtttccaat agttctcttg
    17401 ccgataggca ggaactttct gaccaaattt catccggatc gtttccgcca ctaaactatt
    17461 ggaacctaag ctattagaaa tcaaaggttt aggaacgatc gcttttttaa aagaagaaag
    17521 tccttctttt tcgcagcttt tacaacaata ggtatgttga taatgggtga gacaatccaa
    17581 catagcctga tggaaacgaa tttcttctcg tacttttgtc cgtccgatat ctttcatctg
    17641 tgaaccgcaa tgttcacaaa attgttcttc caaaggaagg agatgttctt tctctgttat
    17701 aggtaagtgt ttgattttgt ctgctttcgt tcctacaact cgttgatgtc cttttactgg
    17761 aataatggtg gcagctgctt ctggaacact tacagaaatt tcttcatcac taaaaagatt
    17821 caattgccca ttcgtttcct caaagagagt ctctttagag cgtccaaata atttgttggt
    17881 taaataattg accttttcat tcaatagttg gatttcagtt gtaagattct gaatggtttg
    17941 ttgttgtgct tgattggttt tatgggcttc ttgaagcaac tgaagcaacg tatctgtagt
    18001 ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt tacagaatac cacaagaaac
    18061 gcacaagaaa taggggttca acacattttt tgtgttaacc ccccagctta aaaaactcct
    18121 ttttttcctg gcaaaatagc tcgaggttga tcgatactta atccttctaa caaccatcga
    18181 acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc gaggccattt taacttgccg
    18241 ttttctatcc gcttataaca gagaagaaaa ccatcaccgt cccaatagag aagtttgaaa
    18301 cggtcttgac ggttcccgca aaataaaaaa acggcatctt cgtaaagctc gagttgatac
    18361 tcttgttgaa taagactggc aagaccatca attccttttc gcaaatctgt ttttccgcac
    18421 acaatataaa tatgtttggt ttgaagtaaa tcataaagca ttggacaaca gttctttcaa
    18481 aatagcttga agaatataat tgttaatacc gttatagatc aaaacttcag aaccattctc
    18541 tcttttgatg cgcatcgcta acttggcagc cgcgggttta cctttttctg gtgcactctt
    18601 ttcgtttcct aattggacag gaacaatctc atttaattta tacatagaaa acctccagac
    18661 ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg ttctatgggg
    18721 cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata tttacgtctt
    18781 attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct ttttcagcct
    18841 attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat ttccattaaa
    18901 gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca tttgttctgc
    18961 aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga tttcagggct
    19021 agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat tgattaggta
    19081 gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag ctagtttggc
    19141 ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag tgtagctaag
    19201 aataaattgt tctacaccat gatttttttg aataactttc cactttttta gcacttcaat
    19261 tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt aaggtttccg
    19321 aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact ttttacgttt
    19381 gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac cagtcatgta
    19441 atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat aaatttttgc
    19501 caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc cttttggaat
    19561 tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg catagtctaa
    19621 tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc cgctatcgtt
    19681 caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa gcttcttatc
    19741 accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag tacttttttg
    19801 tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt aagttaaatc
    19861 ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga cttctttgtg
    19921 tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt tcttctggat
    19981 cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc gaccattttt
    20041 atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga aagaggaaca
    20101 cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt attataatat
    20161 ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt agcttgatgt
    20221 gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat tgtataaact
    20281 cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt ttgtataatg
    20341 aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat ttaaagctaa
    20401 agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta tatcttgatt
    20461 cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt tgagattccg
    20521 ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat cgaaatattt
    20581 gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga tcttaacgaa
    20641 accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat caaacaagat
    20701 aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat actttaaatt
    20761 tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt taaattcatt
    20821 atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa atttcgtttg
    20881 attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt gataatgttt
    20941 ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat agttttttct
    21001 tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc gttccagatt
    21061 tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat cataaatttc
    21121 aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc atcactcctt
    21181 gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc tataaatcaa
    21241 gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg gcggtcttaa
    21301 cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac ccaacactcc
    21361 atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag ttgccgtatc
    21421 agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc aaggtacaat
    21481 caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt ccggcgtgat
    21541 cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt ttggtaggat
    21601 acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga cattgacgac
    21661 ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt ttattggcat
    21721 cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata tagatggcca
    21781 tctacgactt gtattatata cgagaactta tacgtagtca agagattttc taaaaaaata
    21841 tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa tttgaatatg
    21901 tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga aataaaagac
    21961 ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa gccgacgatc
    22021 agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca attatctagt
    22081 atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata tatccgagag
    22141 tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat cattgagaaa
    22201 ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa tgagctaggc
    22261 tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga aatcatgaag
    22321 gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct aaacaaacta
    22381 tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat tgactatatc
    22441 gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat ggtgaaagaa
    22501 tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta tttgattgga
    22561 aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc ttctttatca
    22621 atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt agtcgaaata
    22681 tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc agatgaattt
    22741 tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt ggtgaaaaaa
    22801 tttttttcag cataacatca gtaatctata aaggggttgt tgattatact gtagaagtta
    22861 agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt tcactagaaa
    22921 atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt taaaaatgag
    22981 aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag attaaactta
    23041 ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta ttggatttta
    23101 taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa tctgtatata
    23161 ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt gtttaaaata
    23221 ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat attgacttaa
    23281 aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa atgcatcttc
    23341 gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat tgggaggaga
    23401 ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa cgggataaaa
    23461 aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag tggatgtact
    23521 tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca atgtacggtg
    23581 ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt ccttgggtag
    23641 gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg ctgagcaaca
    23701 cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt attttagcac
    23761 tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg attctatatg
    23821 cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc ctatccgcaa
    23881 atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg ttcatgtcta
    23941 atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga atcggctggt
    24001 catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat gaggcagcaa
    24061 atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct gcattgaggc
    24121 ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta ggatatgaaa
    24181 aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt tccacttatg
    24241 tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt ggtctgttca
    24301 atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag cggctaaacg
    24361 atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca aaaatcgacc
    24421 gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc accattgttc
    24481 cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc aataaaggta
    24541 taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt gcagatgatt
    24601 ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc ttattgactg
    24661 tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg gggaaaaaga
    24721 ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata cctacctatt
    24781 tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta ccaggtgcta
    24841 taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca aaagaacttt
    24901 ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa gtcatgcttc
    24961 cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg caatggaact
    25021 catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg caactcgtat
    25081 taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc aatactgcca
    25141 tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc atttctagtc
    25201 tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt gttttagttg
    25261 gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat aaaggagatt
    25321 ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta attttggggg
    25381 cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta gaaaatgtct
    25441 cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc cctttggctc
    25501 ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc aatctccata
    25561 ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat atcgctagtg
    25621 gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta ttaaactggg
    25681 cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg ccaaatctga
    25741 agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct gatggtcaca
    25801 tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt catacagtaa
    25861 atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa atgccgcaaa
    25921 ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca aatggtaatg
    25981 gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa gacttaaaat
    26041 ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt gataacgatg
    26101 gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa tatttcaatg
    26161 aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg aatacgtatg
    26221 ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa gcaaatatca
    26281 cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat ggaacgaaac
    26341 atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt actagtgcga
    26401 ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg ttacagtctg
    26461 tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt gaattaaagg
    26521 atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta agacaaaaaa
    26581 ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc actactatgc
    26641 cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca tatattacag
    26701 aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga ttagcaaaaa
    26761 tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc accggcggta
    26821 aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt ttggaagaat
    26881 ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa taagtgttag
    26941 tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg cgaggaaaag
    27001 ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg ataaatgacc
    27061 ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac tatccttacg
    27121 atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg gtccattggg
    27181 aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg tgtttctcag
    27241 gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt caagaaattg
    27301 atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt cattttagca
    27361 aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg ctaatacaag
    27421 aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta gtagctacta
    27481 aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg tatacttggc
    27541 acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg gaatgtcctg
    27601 atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa atcaagaaga
    27661 acggactgga atatcataat atcagttcta ccatggcttg tattggtgag gtaaactggg
    27721 aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg gatttttatg
    27781 caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg atgcagatgt
    27841 gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct atgagtttgc
    27901 cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca actgtatata
    27961 gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca tcagtcgtat
    28021 ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg gagaaagctg
    28081 aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta gcctatgaca
    28141 ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt acaggggcgg
    28201 aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat cagctcgttc
    28261 ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta gaagttatga
    28321 cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg atcggagaaa
    28381 gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc ttaggcgatg
    28441 aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag atagctagat
    28501 ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca aaattcatta
    28561 tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga aagatgtcgc
    28621 aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg gttctgttaa
    28681 agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc aaccagattt
    28741 ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc tacccacggt
    28801 atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag aaagacaaaa
    28861 ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag aatatatcca
    28921 gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag atatcgatga
    28981 atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg aagaggtgat
    29041 ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac tttatcgccg
    29101 aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg aaacgaagag
    29161 tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata aagcgttaga
    29221 tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt tagagaatcc
    29281 cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca ttgatatgct
    29341 tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg atggtcaaaa
    29401 gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa agcaaatggc
    29461 ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag aaaaacgtac
    29521 cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga gaaacgattt
    29581 tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg tagataaaat
    29641 gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg gtaatgaaca
    29701 attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa cgatggagca
    29761 agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg taggagcatt
    29821 tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca acacaccgaa
    29881 gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt cggttcccat
    29941 ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag ggtatggaaa
    30001 aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg gtgctgtagt
    30061 ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc tcttacaacg
    30121 tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt tggaagggtt
    30181 agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta tctcatttga
    30241 tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata acacaacgtt
    30301 actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc cgcaactact
    30361 cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata cgcctactct
    30421 cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg gctgcctagc
    30481 tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt cataacatac
    30541 cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt ctttcttgag
    30601 aatctgagta gagactttta ttctgtttca aattaatata attacgaaat gatattgagt
    30661 acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat atatttatag
    30721 tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt agtgaataaa
    30781 agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa tggtaggaaa
    30841 ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg atttataggt
    30901 attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag gtggtttgct
    30961 ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac ggacactgat
    31021 tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt caatagaagt
    31081 gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa ccccaacaga
    31141 gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac cggcattggc
    31201 gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt cttctgtcaa
    31261 ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc aaacgatgct
    31321 gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca atgtgttcaa
    31381 tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt gatgtgattt
    31441 gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg ttccttgtgc
    31501 aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga agacatatac
    31561 aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag caagttttga
    31621 taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc cgccaagact
    31681 ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga agatacatgt
    31741 taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat ctactccgcc
    31801 tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg cgatctccat
    31861 gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta gtgtaaaatg
    31921 gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt ggattccgta
    31981 cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg tagcaacgac
    32041 atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa cggtttgttt
    32101 ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt ctgcttttcc
    32161 gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa tcaactgcat
    32221 accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat caataccaaa
    32281 ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt tagggttcat
    32341 tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa caccgaactc
    32401 acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta cgataatttt
    32461 tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag tccgatatgc
    32521 ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacctt gttgtaaata
    32581 gatccgtgcc cgaaagttat aaaagttgcg ataaccaaac gcaattcgtt taatcagctt
    32641 gatcttgtta ttcagcccct cagtgacgcc gtttgagtgg gacaccttaa aggcatttga
    32701 aatacccgat tggtagcgat ggaagatcgt gaatttcttt ttaaacgact gaggtaacgt
    32761 agatgaaatt gttttaaggg tttcttgaaa gccttccgta
//
LOCUS       DAYYAG010000018        32065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000018 DAYYAG010000000
VERSION     DAYYAG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.018"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..661
                     /locus_tag="ACHARQ_000735"
     CDS             <1..661
                     /locus_tag="ACHARQ_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409185.1"
                     /translation="ADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFENFGLTAF
                     IDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNMIDSTEC
                     LFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMSTEDVRS
                     IIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYKNTIFTN
                     L"
     gene            694..1371
                     /locus_tag="ACHARQ_000736"
     CDS             694..1371
                     /locus_tag="ACHARQ_000736"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8409186.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            1859..2824
                     /locus_tag="ACHARQ_000737"
     CDS             1859..2824
                     /locus_tag="ACHARQ_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8409187.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            2846..3058
                     /locus_tag="ACHARQ_000738"
     CDS             2846..3058
                     /locus_tag="ACHARQ_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8409188.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3133..3609
                     /locus_tag="ACHARQ_000739"
     CDS             3133..3609
                     /locus_tag="ACHARQ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8409189.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            3606..5414
                     /locus_tag="ACHARQ_000740"
     CDS             3606..5414
                     /locus_tag="ACHARQ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8409190.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            5491..5706
                     /locus_tag="ACHARQ_000741"
     CDS             5491..5706
                     /locus_tag="ACHARQ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8409191.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            5798..6661
                     /locus_tag="ACHARQ_000742"
     CDS             5798..6661
                     /locus_tag="ACHARQ_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8409192.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6698..7234
                     /locus_tag="ACHARQ_000743"
     CDS             6698..7234
                     /locus_tag="ACHARQ_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8409193.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            7248..7670
                     /locus_tag="ACHARQ_000744"
     CDS             7248..7670
                     /locus_tag="ACHARQ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8409194.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            7600..9999
                     /locus_tag="ACHARQ_000745"
     CDS             7600..9999
                     /locus_tag="ACHARQ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8409195.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            10031..12022
                     /locus_tag="ACHARQ_000746"
     CDS             10031..12022
                     /locus_tag="ACHARQ_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8409196.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            12045..12296
                     /locus_tag="ACHARQ_000747"
     CDS             12045..12296
                     /locus_tag="ACHARQ_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8409197.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12286..13515
                     /locus_tag="ACHARQ_000748"
     CDS             12286..13515
                     /locus_tag="ACHARQ_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8409198.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            13512..15593
                     /locus_tag="ACHARQ_000749"
     CDS             13512..15593
                     /locus_tag="ACHARQ_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8409199.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            15742..19662
                     /locus_tag="ACHARQ_000750"
     CDS             15742..19662
                     /locus_tag="ACHARQ_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8409200.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19663..20607
                     /locus_tag="ACHARQ_000751"
     CDS             19663..20607
                     /locus_tag="ACHARQ_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409201.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            20611..20814
                     /locus_tag="ACHARQ_000752"
     CDS             20611..20814
                     /locus_tag="ACHARQ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8409202.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(21114..21350)
                     /locus_tag="ACHARQ_000753"
     CDS             complement(21114..21350)
                     /locus_tag="ACHARQ_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409203.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(21533..21904)
                     /locus_tag="ACHARQ_000754"
     CDS             complement(21533..21904)
                     /locus_tag="ACHARQ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8409204.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(21901..23229)
                     /locus_tag="ACHARQ_000755"
     CDS             complement(21901..23229)
                     /locus_tag="ACHARQ_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8409205.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(23190..23519)
                     /gene="mobC"
                     /locus_tag="ACHARQ_000756"
     CDS             complement(23190..23519)
                     /gene="mobC"
                     /locus_tag="ACHARQ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8409206.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(23777..24147)
                     /locus_tag="ACHARQ_000757"
                     /pseudo
     CDS             complement(23777..24147)
                     /locus_tag="ACHARQ_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            24292..24477
                     /locus_tag="ACHARQ_000758"
     CDS             24292..24477
                     /locus_tag="ACHARQ_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8409207.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            24635..24802
                     /locus_tag="ACHARQ_000759"
     CDS             24635..24802
                     /locus_tag="ACHARQ_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409208.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            25420..26079
                     /gene="vanR-B"
                     /locus_tag="ACHARQ_000760"
     CDS             25420..26079
                     /gene="vanR-B"
                     /locus_tag="ACHARQ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8409209.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            26082..27443
                     /gene="vanS-B"
                     /locus_tag="ACHARQ_000761"
     CDS             26082..27443
                     /gene="vanS-B"
                     /locus_tag="ACHARQ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8409210.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            27598..28404
                     /locus_tag="ACHARQ_000762"
     CDS             27598..28404
                     /locus_tag="ACHARQ_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8409211.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            28422..29129
                     /locus_tag="ACHARQ_000763"
     CDS             28422..29129
                     /locus_tag="ACHARQ_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8409212.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            29126..30097
                     /locus_tag="ACHARQ_000764"
     CDS             29126..30097
                     /locus_tag="ACHARQ_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8409213.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            30090..31118
                     /gene="vanB"
                     /locus_tag="ACHARQ_000765"
     CDS             30090..31118
                     /gene="vanB"
                     /locus_tag="ACHARQ_000765"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8409214.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            31124..31732
                     /locus_tag="ACHARQ_000766"
     CDS             31124..31732
                     /locus_tag="ACHARQ_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8409215.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(31798..31971)
                     /locus_tag="ACHARQ_000767"
     CDS             complement(31798..31971)
                     /locus_tag="ACHARQ_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409216.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         8258 a         8100 c         8614 g         7093 t
ORIGIN      
        1 tgcagacgaa attattaaag attggtttcc aactataaaa gcagatattt ttatatctca
       61 ctctcatgct gatgaaaaat tagctatacg ttttgcatca tggctatttg aaaattttgg
      121 tcttactgca tttattgatt cttcggtttg gggatacagt tctgatttat taaaaaaaat
      181 agaccaaaaa tattgctata aggaacaaac aaaaacatat gattatgata aaagaaatgt
      241 aacaactagt catgttcata tgatgctttc tacagcttta aataatatga ttgatagtac
      301 agaatgtctg ttttttctta atacacctaa ttcaattagt ttgagcaatg aaatcactaa
      361 tgaacagaaa tttacgtatt cgccatggct atatagcgaa ctgacgacag cttcaatagt
      421 ggaaaaaaag aatccacgat tggaaagtaa tcctcaaatg agcaccgagg atgttagaag
      481 cattataaag cactattctt cgcaagaaaa tctgcaaata aaatattctt taaatttaag
      541 tgaattaact tatttacgaa aagctgattt gaaaaaatgg gctttagatg ctgtggaatg
      601 cactaaagga agtaatacat tagatatttt atataaaaac accattttca ctaatctcta
      661 atcaatttac tgaatcgaaa ggaaccgggt agaatggata agaaagtttt aataaagagg
      721 ttctctgaag ctattgaaaa aggaaatgca gcaatatttg caggtgcagg attatcgatg
      781 tcacaaggat atgtaagttg gccggaacta cttaatgatc ctgcaacaga aatcgggttg
      841 gattctaaaa aagaaacaga cttagttact ctggctcaat attataaaaa tgaaaatggt
      901 ggatcaagag gaattttgaa tcagatactt atggataatt ttggtgaaga attagaaata
      961 tctgaaaatc atcgaatact agcaagttta cctattgaaa cttattggac aactaattat
     1021 gatcatctaa tagaaaaatc aatccgagaa gcttataaaa accctcaggt taagaaaaat
     1081 tatactcagt tagcaacaac taatccaaat gtggatacaa ttgtttataa aatgcatggt
     1141 gatatagatg atgtcagttc gacagtaata acaagagatg attatgaaaa atatgatgat
     1201 tcgtacgcgt tatttaaaga aactttaaaa ggagatttgt taacaaaaac ttttctcttt
     1261 ttaggattta gttttacaga ccctaattta gaaagaattt tatcagatat aagatgggtt
     1321 ttgagagaaa accaaagacc tcattattgt ataatgagaa aaatattata gaaaaaattt
     1381 taggttatcg ctgaaagccc cggaaatacg ggcatttcgc aggatatgag aactcaattt
     1441 actaccaatt tactactttc ggattgagcc gcatatatgc tgaaccatac tgctaatacc
     1501 cgccacccca aatacggcac atcataaatt gaatacgaca ccgtaaacgc ggcgttcctc
     1561 tttcccgtca tgggagaaca ggacgccgct tttttatgcc caaatagaga aaggaggcga
     1621 cgccatgcta tttacttccg gccctgaccg ggcgtttgaa cggctgatgc aggaaaagcc
     1681 cggtttcgat cactacgacg gcggcggggc ggacgcgccg gaggattgcg gcacctgccg
     1741 attttaccgc ccggattgga aatatcaatt ctgccagttt gcagagtgtc cctatcagcc
     1801 gggcaagctg accgactatg cagcggtcaa attcacagtg aaaggagcga acaacgctat
     1861 ggcagttttc cgtgtggaaa agaacaaagg ctatacggtg atgtcaaatc accacctgcg
     1921 gaataagaac ctgaccttaa aggccaaggg cctgctgtcg cagatgttgt ccttgccgga
     1981 ggattgggac tataccctaa aaggactgtc ctatatcaac cgggaaagca tcgacgcgat
     2041 caggacagcg gtatgggagc ttgaaaaagc tggatatatc cggcgtgagc agggccgtga
     2101 cgcaaaaggc aaaatggccg atatggtcta taccatctac gaacagccgg tattggaaaa
     2161 cccggtattg gaaaatccaa catcggataa cccggtgttg gaaaatccga catcggataa
     2221 tccggcgtcg gaaaatccaa cgcaattaaa taaagaagta caaagaactg acttatcaaa
     2281 aaaagaaaaa ataattacag atggacaaag tacccattcc attcctatcc catccccgac
     2341 ccctgcccct atggagggcg aggcagcggc tacgccgccg gaacggaaaa ggacaggaaa
     2401 ggatgaggct gtacggattt atcgggaaat cattttggag aacatcgagt atgcctatct
     2461 cattcaggac aggagcattg accgtgaaca gcttgacgaa atcgtagacc tgatgctgga
     2521 aaccgtctgc accgcccgaa agacaatccg tgtcgccggg gacgactacc ctgccgagct
     2581 ggtgaaatcc aagtacatga agctgaccgg cgaacatatc cgctttgtcc ttgactgtct
     2641 gcgggagaac acaacgaaag tccgcaatat caaacaatac ctgcgggcta tgctgttcaa
     2701 cgcccccagc accatttcca actactacac ggcccttgtc gcgcacgaca tggcacagcc
     2761 cgattgggga aagcctaaat ccggcctgcc ggactattcc tgcggcgagg gcgaaagcct
     2821 gtaacgagaa aaggaggacg accctatgaa gcagggcgca ttgatttttg acgagcgaac
     2881 agaccgttac gacatccgct ttgatctggc cgactattac ggcggcctgc attgcgggga
     2941 gtgtttcgac gtgatgatcg gcggcaaatg gaggcccacc cgcattgaaa tggccgaaaa
     3001 ctggtatctt gtcggcatcc gggccgacga cctttccggc ctgcgggtgc ggatttgacc
     3061 tatatctacc tgcggcgtct ggtggttccg ccttagacgc cgcttttgac tttctgacaa
     3121 ggagggatgc gattgcagga agaagttact caaaagacca ttgccctgtc catgaaaacg
     3181 ggcaagctca cggctcaagt gctgcaagcg gcgctgaaaa agtacctgca acaccgggcc
     3241 aagggcaaaa acacgctgca ccacggccag cagagcctaa agcagcttaa aaagcatggg
     3301 gctgatctct ccaacattga gatcaccgag gccaacattg gagcgttcaa gccatgcgcc
     3361 aagaaatacg gcgtggattt taccttacga aaagataaga ccacccagcc gccccactac
     3421 gttgtgattt tcaaggccaa ggatgcagat aaccttgatc tggcgtttcg ggagtttacc
     3481 gccaagacgc tttccaagga gcagcggccc tctatccgca aggttttgtc cgctgtgaag
     3541 caaaaggcgg cggcccagac caagcagcgg gccaaggaga aaatcaagga aagggggctg
     3601 gaactgtgaa gcctgacgta aagaaattgc tgatcctgaa cctcccgtat ctgatttttg
     3661 tgtacctgtt tgcgaaatgc ggggaggcgt accggctggc cgctggtgct gacctgtcgg
     3721 ggaagctcct gcattttgcg gatggttttg cggcggcttt tgccaaccct ctgccaagcc
     3781 tgcacctgtt tgacctgtgt atcggtatcg ccggtgccgt gatcgtgcgg ctggtggtgt
     3841 actgcaagag caagaacgtg aagaaatacc gcaaaggcat ggagtatggc agcgcccgct
     3901 gggggacggc caaagacata gcgccttaca tcgaccccaa gtttgaaaat aatatcctgc
     3961 tcacccaaac ggaacggctt accatgaccg gcaggcccaa agaccccaag acagcgcgga
     4021 ataaaaacgt gctggtgatc ggtggcagcg gttcagggaa aacgagattt ttcgtgaaac
     4081 ccaacctgat gcaatgcttt ccgaccacgg actatccgac ttcgtttgtg gtgacagatc
     4141 cgaaaggcac tttggttctg gaaaccggca ggatgttcca gcaggcgggc taccgcatta
     4201 aaatcctgaa taccattaac ttttccaagt ccatgaagta caaccccttt gtctacatcc
     4261 attcggaaaa ggacgtgcta aagctggtaa ataccctgat ttccaacacc aagggcgagg
     4321 gggaaaagtc agcggaggat ttttgggtga agtcggaacg gttgttttat tcggccctga
     4381 tcggctatat ctggtacgag gccccagcgg aggaaatgaa cttctcaacg ctgctggaaa
     4441 tgataaacgc cagtgaagcc cgcgaggacg acccggagtt tcaaagcccc gtcgatctca
     4501 tgtttgagcg tttggagcag aaagaccccg accattttgc ggtgcgccag tacaagaaat
     4561 tcctgctgtc cgcaggcaag acccgttctt ccatcctgat ttcatgcggt gccagacttg
     4621 cgccctttga catccgggag gtacgcgagc tgatggagga cgacgaaatg gagctggaca
     4681 ccatcgggga cgaaaagacg gtgctgtatc tcattatgag cgatacggac acgaccttta
     4741 atttcattct ggctatgctc caaagccagc taatcaacct gctttgtgac cgggctgatg
     4801 atgtgtacgg cgggcggctg cctgtccatg tacgcctgat tttggatgaa tttgccaaca
     4861 ttggccaaat acccaatttc gataaactca ttgccaccat tcgaagccgt gaaatatcgg
     4921 cctctattat tttgcagagc cagtcgcagc taaaagcgat ttacaaggac gccgccgaga
     4981 tcatttcgga taactgcgat agtgtgcttt tccttagtgg gcgcggcaaa aatgccaagg
     5041 agatttccga tgcgctgggg aaagaaacga ttgacagttt caacaccagc gagaaccggg
     5101 gaagccaaac gtcccacgga ttgaactatc aaaaattagg aaaggagttg atgtcccaag
     5161 acgaaattgc cgtgatggac ggcggcaagt gtatcttgca gctacggggt gtgcgcccgt
     5221 ttttatcgga gaaattcgat attaccaagc atccccggta caaatacctt gccgacgccg
     5281 acaagaagaa tacctttgac gtggacagat ttttgactat cacgcgccgg aaacggcagc
     5341 aagtggtaac gcaggatgaa gtattcgacc tgtatgaaat cgacctgtcg gacgaggacg
     5401 ccgccgctga ataaacagcg gcttttttca ttccaacaac aaaatcaaaa aatcattatg
     5461 agccggaaac cggcagaaag aggtaaattt atggcatttt ttaattccgc agttactact
     5521 ttgcagacta tcgtgatcgg actgggcggc gctctgtgcg tatggggcgg catcaatctg
     5581 ttggagggat acgggcagga caatccgggg gcaaaatcgc agggtgtcaa gcagcttgtc
     5641 gctggcggcg gcgtggccct gatcggtgtc actctggttc ccctgctgtc cgggctgctg
     5701 ggctgatcct ggcctttgtc cctgtccaaa cccttaacta atcgcctccg cgtcctcccg
     5761 gccagaccgg gagggcgcgg gaaaggaggt gctacccgtg attagtgaaa tcattgaaga
     5821 atggataaaa ggcatcctca tggacggtat cacgggcaac ctgtccgggc tgttcgatac
     5881 ggtaaatgcc aaagtcggag aaatcgccgc cgacgtggga cgaaccccgc aggcatggaa
     5941 cagcggtatt ttcaatatgc tgcacagcct gtcggaaacc gtgatcgtcc ccatagccgg
     6001 ggccatactc gcccttgtca tgtgctatga gcttatccag atgataacgg aaaagaacaa
     6061 tatgcacgac tttgacagct ccatgttctt ccgctggata ttcaaatccg cgtttgcaat
     6121 cctgatcgta acgaacacat ggaacatcgt catgggcgta ttcgacgcca cccaaagcgt
     6181 cgtcaatcaa agcgccggtg tgattatcgg gcagacatcc attgatttta acaccctgat
     6241 accagacctt gaaagccaat tggaggctat gagcgtgggc gctcttttgg ggctgtggtt
     6301 ccaaaccctt gttgtgggcc tcaccatgaa tatcctttcc atctgcattt tccttgtgac
     6361 ctatggccgc atggtggaaa tctatgttgt cacgtcgctg gggcctatcc cccttgccac
     6421 aatgggcaac gcggaatggc gcggcatggg ccaagggtat ctgaaatccc tgctggcgct
     6481 gggcttccaa gcctttttaa tcatggttgt ggtgggtatc tacgcggtgc tgatacagaa
     6541 tatcggttcc gcttccgaca tatccggcgc aatctggggc tgtatgggct atacggtgct
     6601 gctgtgcttc tgcctgttca agaccggcag tatatcaaag gccgtattcg gggcgcattg
     6661 atcccaaaat ccaagcatac acaggaggaa cttgtttatt tcaacgtaca acatcattta
     6721 cgctgacccg ccgtggcggt atgactgcaa aaacgtacag ggcgcagcgg aaaatcacta
     6781 ttccaccatg agcattgacg agctgtgcgc cctgccggtg gaacggctgg cgtccaaaga
     6841 ctgcctgctg tttttgtggg ccacattccc catgctgccg gaagctctgc ggttaatcaa
     6901 agcgtggggc ttttcattca agaccgtggc ttttgtatgg ctgaaacaga accgtaaaag
     6961 ccttacatgg ttttacgggc tgggccgctg gacaagggga aacgcggaaa tctgcctgct
     7021 ggcaacacac ggcaagccga agcgtcgttc tgcctctgtc catcagttta ttatcagccc
     7081 gattgaacaa cacagcaaaa agccggatgt gacccgtgaa aaaatcgtag agctggcggg
     7141 cgatttgccc cgcgtggagc tgtttgccag acagaaaacc cccggctggg acgtgtgggg
     7201 gaacgaggtg gaatgtgacc ttatcctgac gtgaaaggag gtgccaaatg gcttatgtaa
     7261 ccgtccccaa ggacttaacc catgtgaaaa gcaaggttgt gtttgggctt accaaacggc
     7321 aactgatctg tttttccggg gcgctggtga tcggcgtccc cttatatttt ctcacccgtg
     7381 actatcttac caacagcgcg gcggccctgc tcatggtatt tgccatgctg ccgggctttt
     7441 tgcttgccat gtacgagcgt cacggccagc cccttgaagt ggtgattatg cagatcatcc
     7501 agtgctgctt catccagcca aaggagcgtc cctatcagac cgacaacacc tacaccgccc
     7561 ttgtgcggca ataccagatg gaacaggagg taaaggccat tgtccaaaaa ggaaatccca
     7621 agagaaaccg aaaaaacaaa gctgacccgc gcccagcgaa aagaaattga cgcggtaatc
     7681 cgtaaatata agggcgacgg caggccccac acggcgcagc agagcatccc ctacgaggtc
     7741 atgtacccgg acggcgtgtg ccgggtatcg cccggcgtct tttccaagtg cattgagttt
     7801 gcggacatca gctatcagct tgcccagccg gacacgcaga cggctatctt tgaaaaactg
     7861 tgcgacctgt ataactatgt ggacgcctcc attcatatcc agttttcttt cctcaatcgg
     7921 aaagttgacc cggtgcagta cgccaagagc tttgagatcg cgccgcaggg ggacgacttc
     7981 gacgacatcc gggccgagta caccggcatt ctgcaaaagc agcttgcaaa cggcaacaac
     8041 ggcatggtaa agaccaaata cctgacattc accatcgagg ccgaaagcgt gaaagcggcg
     8101 cgagccagat tgaagcgcat cggctttgac ctgttaggct atttcaagag catgggggcc
     8161 gtagcccatg tgatggacgg ctgggaacgg ctgaacctgc tgcatggcgt gtaccacccg
     8221 gacggcgaga tattcaattt tgactggaag tggctggccc cgtccggcct ctccaccaag
     8281 gattttattg ccccgtcctc cctgtgcttc ggcaatgcaa agacattcgg catgggcggc
     8341 aaatacggcg cggtaagttt cctgcaaatc ctgtccccgg agctgtcgga cgatatgctg
     8401 gccgacttcc tcaatacgga aagcggtgtt ttagtcaatc tccatgtgca ggccattgaa
     8461 cagaccaagg cgatcaagac catcaagcgc aaaattaccg accttgacgc tatgaagatt
     8521 gcggagcaga aaaaggccgt ccgcagcggt tacgatatgg acattcttcc cagcgacctt
     8581 gccacctacg gcgaggacgc caagaagctg ctgacaaagt tgcagacccg caatgaacgg
     8641 ctgttccagt tgacgttcct tgtcctcaac gtggccgaca ccaagcaaaa gctgaataac
     8701 gacgtgttcc aagccgcagg cgtggcccaa aagcataact gtccccttgt ccggctggac
     8761 taccagcagg aacagggcct tgcaagcagc ctgccgctgg gcgtcaatca gatcaagata
     8821 cagcgcagcc ttacgacttc cagcgtggcc gtgttcgtcc cctttgtgac gcaggagctt
     8881 tttcagggcg gcgcggccat gtattacggc atcaacgcca agtcccgcaa catgattatg
     8941 ctggacagga agcaggcccg ttgccccaat gccttgaagc tcggcacccc cggcagcggc
     9001 aaatccatga gctgtaaatc cgagatcgtc agtgtgttct taacgacgcc tgacgatatt
     9061 tttatttcag acccggaggc cgaatattac ccccttgtca aacggctcca cggacaggta
     9121 atccggctgt cgcctaccag caaggacttt gtgaacccgc tggacatcaa cctgaattat
     9181 tcggaggacg ataacccgct ggccctgaaa tccgactttg tgctgtcgtt ctgtgagctt
     9241 gtcatgggcg gcaagaacgg gctggaagcc attgaaaaga ccgtgataga ccgggctgtg
     9301 cgcgtgatct accggcccta tctggcagac ccccgcccgg agaatatgcc gatcctgtcc
     9361 gacctccaca aagcgttgtt agatcagcat gtcccggagg ctgaccgggt ggcgcaggcc
     9421 cttgacctgt atgtgtccgg ctccctgaac gtatttaacc accgtaccaa cgtggacatc
     9481 ggcaaccggc ttgtgtcctt tgacattaag gagctgggca agcagctcaa aaagctgggg
     9541 atgctcattg tccaagacca gatatggggg cgcgtcacgg ccaaccgaag ccaaggaaag
     9601 gccacatggt attttgccga cgagttccat ttgcttttga aagaggaaca gacggcggcc
     9661 tattctgcgg agatttggaa gcggtttagg aaatggggcg gcattccaac aggtgccacc
     9721 cagaacgtca aagacctgct ttcctccccg gaaattgaaa atatccttga aaacagcgac
     9781 ttcattacgc tgctcaatca ggcgtccggg gatagaaaaa tccttgcgga acgcttgaat
     9841 ttgtccaccg aacagcagaa gtacatcgac aattccgaac ccggcgaggg ccttttgatc
     9901 tttgaaaacg tggtgctgcc ctttaccaac cccatcccgc acaacaccca gctttacaag
     9961 attatgacga cccgcttaaa cgaggtggcg ggagtatgac ccggcgacgc cacaaaggag
    10021 gtgaaccacc atgaaagagc tgaaagccaa ggcgaaagtg acgcaggcca tgacccgcga
    10081 cgggctgacg ctggaaaatc aggccaccgg cgaggtggag aacatttcca gccgggaggc
    10141 cgaacaggat ttgtccgcgc cggggccggg cggcaccgcc gataagctgc tggaacgggc
    10201 cgacgccatc catgacggcc ataaggccaa gaaagccgcg aagcaggccg gggctgtggc
    10261 cgccgagggg gacgctgccc tgcaccggcc atccgcccgg ctccaactgt cggaggaaga
    10321 acgggccgac ccggatttac agccctatat ccgaaaatcg gacaaacggg ccgataagct
    10381 ggataaggcc cgcgccgcca tccccaagaa gcgcgtcccg gtcaaggaaa ccgtctttga
    10441 cgaggcaagc ggcaaagcaa agaccgtcct gcactttgac aaacaggaca aggggcctcc
    10501 ctccctgaaa cccaatccgg caggccgtcc tgtttccgag gtttttctat tcggccacgg
    10561 gaaaatccat gaagtcgagc atgaaaacgt gggtgtggag ggcggccata agggggagga
    10621 acttgccgag cggcaggccg gaaaagccat ccgcagcacc atccggcacc acaggctgaa
    10681 accctactgg gacgcggaaa aggccgagcg caggctattg tctgccaacg cggagtattt
    10741 ctaccagaaa tctttgcggg ataacccgga gatcgcacag gcggccagca accccatttc
    10801 ccggttctgg cagaaacagc agatcaagcg ccagtacgcc aaggccgccc ggacagcggg
    10861 ccagaccgca ggcgcggcgg cacagggcgc ggccaagaca gccggggcct ccacgaaagc
    10921 ggccaaggcc gcagccaaag aaagtgaacg ggcggcctcc tttgtggccc gccactggaa
    10981 aggggcgctg gccgtccttg tgttggggct tttgatgctg cttatcatcg gcggcttaca
    11041 gtcctgcacc gccatgttcg gcagcgccgg aagcggcatc acggcgtcct cttacttttc
    11101 cgaggatagc gatatgttgg ccgccgagga cgcctacgcg gagctggaag ccgagctgca
    11161 agactatctg gacaactacg aggccaccca tgactatgac gaataccatt ttgaccttga
    11221 cgagatcagc catgacccct atgtgctgat ttccattctg tcggcgctcc atgacgggat
    11281 atttaccatc ggggaggtac agggtgattt gtccatgctc tttgacaagc aatatatcct
    11341 gacagaaaac gtcgtggtag aaacccgtta ccgcaccgaa accgatacat ggacggacgc
    11401 ggagggcaac acccatacag acacctacca agtgccgtat gactattaca tctgctacgt
    11461 cacgctggaa aacttcgacc tgtcccatgt gccagtctat atcatggacg aggaaacgct
    11521 aagtttctat tccctgtata tgtccacgct aggaaaccgg cctgatctgt tttccggcag
    11581 ccagtacccc aacgcctcca ccctgacgcc gccgacctac tacgacgtac ccccggaggc
    11641 tttggaggat gccacctttg ccgccatgct caaagaggcg gaaaaatata tcggttatcc
    11701 ctatgtgtgg ggcggcagct ctcccagcac cagctttgat tgcagcggct tcatttcgtg
    11761 ggtaatcaat catagtggat ggaacgtggg acggctgggg gcgcagggcc tatataacat
    11821 ctgcacccct gtttccagtt cgcaggccaa gcccggcgac cttgtatttt tcaagggaac
    11881 ctacgacacc cccggcgtgt cccacgtcgg cctgtatgtg ggcaacaatg tgatgctgca
    11941 ttgcgggaac ccgatttcct acacgaacct tacttcaaac tactggcaat cccattttta
    12001 cagttacggg cgtttacctt aacgccagaa aggagctttc aattatggca accaaacttg
    12061 accgcatcga aaaggacatc cagaaaacca agtctaagat cgcggagttt cagaaacagc
    12121 ttcgggagct ggaaacgcag aaaaccgaac aggagaattt gcagatcatc cagcttgtgc
    12181 gcggcatgaa catgaagccg gaggaatttg ccgcgttctt acgcagcggc gcaatgcagg
    12241 ccgcccctgc cgcaacccct taccatgaac aggaggacaa tgcccatgaa gaataagacc
    12301 agacggattt cccgtattct ccccgcactt ctggccgtga tgctctgcgt cacggccttt
    12361 cccgttaccg cccttgcagg cggcaatgac ccggcaccgg ccccgctgcc ggaggctaca
    12421 accgaacccg ctacgggcgg tttggagcct gaaacagacg aaaccggcac cgccacggac
    12481 gggacagaaa ccgaggccga aaaagatacc ggccctctga ccggcaagga catcaccgac
    12541 ctgttttccg cgctgttcgg ttccaaggtg agcattgccg ccacggacga cggcatccag
    12601 atcaccaccg gcaccgacaa aaaagaacct gaacagaccg gcaccgtcac caccaacggc
    12661 ggcaggctga atgtccgcac cggcgcgggg cttgacaaga ccgcgttcac ccagcttccc
    12721 aatgggacga cggttgaggt agtcggcaca gacggcgact ggattaaaat tctcttgccg
    12781 gagcgcatcg gctatgtcca ttccgactat atgaccgtca gcgaaaagga agtagcggct
    12841 accggcgagg gcggtttttc cctctccatt gacccggagg aaattgcttc tctgctggaa
    12901 ctgttcaatg gcggcggggc cagcgccgcc ctgtccccgg acgggaacct gtcactgatt
    12961 gacgacatcg gcagcgccgc gaaaaatggc aagcagttta tcaccgtgga aaccaaaaac
    13021 ggcaatgtgt tttatctgat tattgaccgg gacgacgagg gaaaagaaac tgtccatttc
    13081 ttaaatcagg tggatgaagc cgatctgctg acgctgatgg aggacgggga aaccgtcacc
    13141 cccgctgctg tctgctcctg taccacgaaa tgcaaggccg gggctgtgaa cacgaattgc
    13201 ccggtatgta aaaccaacct gaccgagtgc agcggcccgg agccgcagga accccagccg
    13261 gaggaacccg aagccccgga ggaagaaccc aagggcggcg cgggcggcct gatcgtgttc
    13321 ctgctggtgg cgctggcggg cggcggtgcg gccctttact atttcaagtt caaaaagccc
    13381 aaggctgaaa ccaccggcag cgacgatttg ggcgagtatg attttgggga ggacgaggat
    13441 ttggaggacg aggaacccga aaccgagttt gacccggaag ctgattttgt gagtgaacag
    13501 gaggacgagg aatgacatat aaacttgtaa tcgcggaaaa acccagcgtg gcccgcagca
    13561 ttgcgggcgt gatcggggcc acggagaaac acgacggcta tttggagggg aacggctatc
    13621 tggtgagctg gtgcatcgga catctggtgt cctttgccga cgccggacgc tatgacgagc
    13681 gtttcaaaaa atggcgctat gaggatttgc ccattctccc ggaaacatgg cagtacatcg
    13741 tccccgacga caagaaacag cagtttgacg ttctctgttc gctgatgcag cgccccgacg
    13801 tgaccggcct tgtgtgcgcc accgacgccg ggcgcgaggg ggaattgatt ttccgtttcg
    13861 tctaccagat ggcgggatgc caaaagccct ttgagcgcct ttggatttcc tctatggagg
    13921 acagtgctat taaggacggc tttgcccacc tgaaaccggg cggcgactat gatccgctgt
    13981 atcaatcggc cctgtgccgg gccaaggccg actggctgat cggcattaac gccacccggc
    14041 ttttttccgt cctgtaccac aagaacctga ctgtgggccg cgtccagacg ccgaccttaa
    14101 aaatgctggt ggacagggac gccaagatta ccgatttcaa gaaagagaaa tatcacatcg
    14161 tccatatcac ggcgggcggc gcggacgcgg taagcagccg cttttccgac gctgccgagg
    14221 caaacaccgt caaggcggct tgtgcaaaag cgcaggccgt ttgcgtttcc gtgacgcggg
    14281 agaaaaagac ggaacagccg cccaagctct atgaccttac caccttgcag cgggaggcta
    14341 accggctttt cggctatacc gccaagcaga cccttgacta tgcccaggca ctgtatctaa
    14401 agaagctgct gacctatccg agaacggata gtcaatatct taccgacgat atgctgcccg
    14461 ccgtggaaag ccttgtgtcc ggcctgtggg agctggtgcc gtttgccaag ggcctgaacc
    14521 tttccccgca gtttgaccgc atcctgaaca gcaaaaaggt gtccgaccac cacgccatta
    14581 tccccaccgc tgaatttgtg aagcagggct ttggcactct ggccgagagt gaaaaaaagc
    14641 tgctgtccct gatctgctgc aagctcttat gtgccgtggc cgggccgcat atctatgagg
    14701 ccgtcaccgc cacatttacc tgtgccgggc aggagtttac cgccaagggc aagaccgtcc
    14761 tttcccccgg ctggaaagag attgaacgcc ggttccgttc ttccttaaag accgacgccg
    14821 acgaggacgc cgaggcggtg cgggagctgc cggagctttg cgagggccag acctttgcag
    14881 acgtggccgc ctctgtcacc gagcatttca ccacgccgcc caagccctac accgaagata
    14941 cgcttttgtc cgctatggaa cgggccgggg ccgaggatat gccggaggat gccgagcgca
    15001 agggattagg cacaccggcc acccgcgccg ccattctgga aaagctggtg cagatgggct
    15061 ttgtccagag aaaaggcaag cagctcattc ccaccaagga cggcatcaat ctggctgtgg
    15121 tgctgccgga ggcgcttact tctccccagc ttactgccga atgggaaaac cggctgaccg
    15181 agatcgccaa ggggcaggcc gatcctgacg aatttatgga cggcatcgag acgcaggcgc
    15241 gggagctggt gcagacctat tcctgtatca gtgaggaaaa gcagaaattg tttcaggccg
    15301 agcgtgttcc tatcgggacg tgtccccgct gtggtgaggc tgtctatgag ggcaagaaaa
    15361 actactactg cggaaaccgg gcctgccagt tcgtcatgtg gaagaatgac cgattttttg
    15421 aggaacgcaa gaaagcgttc accccgaaga tcgccgctgc gctgctcaaa accggcaaag
    15481 tcaaggtaaa gggcctctat tccgtaaaga ccggcaaaac ctacgacggg acggtgcttt
    15541 tggctgatac cgggggcaag tacgtcaact accgcattga acacaggagc taaaggacag
    15601 taacacagca agcataggga gtgtggctac actcccggta aaatcccccg gccgcgtccc
    15661 tgccgggccg gggatttttg cttgttgggg aagtccccaa ccccctattt tttgaaacag
    15721 aacagagaaa ggagtgaccg cgtgaacgaa gtattttcta tccaagtcca taaccggcag
    15781 tttttggaac agggcaaaca aggcgtctgg ctggatttgc ccaccacaac cgaaacgctg
    15841 caagccgccc tgcgggatgt tggcattact gccgacaatc cgcaggattt ttttatcaac
    15901 gggtattcct gcccggagga cagacacctt gccattccct atgacatggt gctggccgcc
    15961 gacgtggacg agctgaattt ccttgccgcc cggctgggcc agcttgacgc cgccgagatc
    16021 gcagagctga acgccgccct gcaaaatccc cgtggcggct ttgagaatat cggccagatc
    16081 atcgactaca ccgaaaatgt ggattactat gtgcatcttc ccgacgttag gggggatgga
    16141 cagttgggcg actactacct gaaccgttcc ggcatggtgg atatgccgga ggaatggaaa
    16201 gccgggattg atacggcgca gtttggacgc cacattgccc agcaggaaca aggcgttttt
    16261 acccagtacg gctatctggt gagaagcggc gacgaatggc agcgcgtcca tgagggccag
    16321 ccggtgccgg aggaataccg ggtgatgcac tacccccagc cggaagctga ccgggaggaa
    16381 ccgcaggccc agcagccgga ggcatccgaa agccccgaca aagcgccgcc cccggttatc
    16441 ccgatcatgc tggacggcat gaacagcaaa gaacgcatga aagtgattac tgaccgtctg
    16501 gaaaccggcg tacaggagct ttttgaaagt agccgctata tggcttactt atccgctatg
    16561 tccaagatgc acgattacag tctgaacaat acgctgctga tcgccatgca gggcggttcg
    16621 attgtggcgg gctataacag gtggcaggat gaatttgacc gccatgtgaa gcggggcgag
    16681 aaaggcatta agatactggc cccggccccg ttcaaggtta agcagcagat ggagaaaacc
    16741 gacccggcca cccagcagcc cgtgatcggc gcggacggca agcccgtgac cgaagaacag
    16801 gaagtgacgg ttccggcctt taaggtggtg acagtctttg acatcagcca gaccgagggc
    16861 aaggattttc ccatgcccta tatcagtgaa cttaccggcg acgtgcagca gtatcaggat
    16921 ttctttgccg cgctggaacg gacatcaccc tatcccataa gcattgagcc gatttcagac
    16981 ggggccaagg gccgctgctt ctacgcaaaa cagcgcattg ccatcaacga gggcatgagc
    17041 gaattgcaga ccctaaagac cgccatccat gagattgccc atgccaagct ccacgccatt
    17101 gaccgggagg cccccgccga ggaacaggcg gcccggccag accgcagcac ccgcgaggta
    17161 caggccgaaa gcgtggccta taccgtctgc cagtattacg ggctggacac gtcggactat
    17221 tcttttggct atgtggccga ttggagcagc ggcagggagt tatccgagct gaaagcctcc
    17281 cttgaaacca tccgggccac ggccagcgaa ttgataaccg agatcgacgg ccattttgcc
    17341 cagcttcagc aggagcgcga ggcccggcag gaacaggagg ccgtcacggt tgacgttacc
    17401 ccggaggcag cggccccggc gcaggccgag gaagaacagg ccccggccct tgatccggca
    17461 gccgagccgc ttgtgactgt gctatggagc gaaagccccc agctaaagga cggccagcaa
    17521 atgccgttgc atgaggccga cgcgctgttt gcccgtctgg atgcagcata tccggcagag
    17581 gcaggctatg acaaaaccgc gttccgcatt gactgtatct ttcagggcga gccggacagc
    17641 tacgaggggc ggcaggattt tggagatagg gacggttccc tgatcgaaca tatccagagc
    17701 taccaccaat tttacgcgca ggacgaaaac tggaaaaagc atgtaatcgc tacaaccggc
    17761 atagagggct gggagcagga caaggcccat cgggatatgc tgttaggcga gtttgtcccc
    17821 tatatgaagc tgcattgcag ccttgcccaa atggaacaga cggcgcaggg ccgcttacag
    17881 tccggggaaa ccctgacgcc ggaggaaacg gcctatttgg gggcgcttct ctcttatgtg
    17941 caggagtgcc gccccatgct caatcagggc cagtatcagt tgccggagcc gccgaaactg
    18001 gctgacttcg accagagctt gcaggactac aaagcacagg tacaggccga gatcgcgcag
    18061 gaggccgccg cagccggtat gaccgtggag gaatacgcgg cggctggcta tgaagcaccc
    18121 caacaggaca ccttttccat ctaccagctt cgaggcgggg aggaaacccg cgactatcgg
    18181 tttgagccat acgagcgttt acaggccgca gggctggccg tggacagtgc caactatgac
    18241 cttgtttata cggcaccgct ggataaaagc acgtctttgg aggatattta ccgcacattc
    18301 aacatcgacc acccggcaga ttttagggga cacagccttt ccgtttccga cgtggtggtg
    18361 ctgcaccaga atggacagga caccgcccac tatgtagaca gcttcggcta tatggaagtg
    18421 ccggagtttt tgcgggaaaa tcccctgcgt accgccgaac tgtctacgga gcagaacgaa
    18481 aacatgattg acggcattat caacaatacg ccgcccgaac cgcagccgga ggaacccgaa
    18541 cagctatctt ttgaggatgt ggcagttgct tccggccaga cacaggagga acccccggag
    18601 gctgcgcccc cgcaaacggc tgtccgctat tatcccatca atgagggagc cgcccgccgc
    18661 gccaaggaag ccatgagctt ttctgactac cggcccggca gcgccacggc ggaataccgg
    18721 cactatgtcg atcaggccgt ggaaattgcg gagcgccaga aaaagcgtgt ggagccagag
    18781 tatcacgaaa agatagacag cctccttgac acctacgccc gcaagctggc cgagaacatg
    18841 aataagggct atgagattac cgcccgcgtc ccctctgtcc tgattgccgg gccgtccaat
    18901 ttcccggtac gggcgaaaga aaagcagaac gccgccagtg accgcaacat ggaggaattt
    18961 cagtacattc agggcttgct tgacaaaatc cgcagcaccg gcatgggtgg tatcagcgcc
    19021 gacgatccgc aggccgtttc caagctggaa aagaaactgg aaaagctgga agcgtcgcag
    19081 gagttgatga aggccgtcaa tgcctactac cgcaagcatg ggacgctgga cggatgcccc
    19141 catctgatcg aaagaggcat tgaaaacctg aaagcggata tggcgtcggg ctggcactat
    19201 gagaaaaaac cgttccagtc ctggcagctc tccaacaata acgccgagat acgccggtta
    19261 aagggccgca ttgaggaatt gacccgccaa aaggaggcgg cctatgtggg atgggagttt
    19321 gacggcggca ctgtggagat caaccgggtg gccaaccgcc tgcaaatctt ctttgagggg
    19381 aagccggatg ctgctgtacg cgacgagctg aaaagcaacg gtttccgctg gtcgcccaag
    19441 gccggggcat ggcagcggca gttaaacgac accacaatcc gcgtcgctga ccgtatcaag
    19501 tgcatccagc ccctttccgg cgaaaagccc agcgccttac agcttgcggc ccgcagggag
    19561 aaagaaaagc cctctatccg ggcgcagctc aacgcggcca agaccgaaca ggccaagcag
    19621 ccaccggcgc gggaaaaatc aaagggattg gaggtagagt aaatgcagaa gtttgaaaat
    19681 gtggacgttc tggccgcgct ggaacagatc atgcggcaga acaccgcgtt ttatcaaagt
    19741 gactttggca ttgacaaaga cattatcagg cgggatgccg ccagcgatca ggccgcagac
    19801 aagaccctgc tctggatgtc ccggccctcc gggacgtact gtttccggga acgtgatgtt
    19861 ttcctgaaag acacccgaca gtacaacaca tggaaatttt acggggagca gacccgtgac
    19921 aagatactgg cctacgcggt ggagctgacc ggcacacagg gcggcactat ccggggcaat
    19981 ctctatgagc tggactatcc gcagcatttc cggcacgtca ttgaaaccgc ccagccggtg
    20041 agtgtgaacc ggctctttta tgagcatggc acccgtgaca tcccggagag ccagcgtttt
    20101 gacggaagcc ctgaccgggt attgggaaac tttttgtact atgaggccca gccccatgac
    20161 ccggctgtgc tgcaggaggc gctacggacg gagcagcaca gccgggggcg ggccgtcccc
    20221 ggcgatttca aagtccacgt caccgctttg catgacagcc ggattgaaac cgaggcccgg
    20281 cgtattgtgg acagcctgaa agatctgtcc gcgcctaaca gccccaataa aacgcatttc
    20341 atggtggaga tctccccgca ctttgcggcg attgcttcca ccaaggataa tgaccggctc
    20401 tttgccatgc tgccctataa atccctgtgt tttaccggga tgaaaggcag gcatggcgtg
    20461 tatgccgtga ttaaccggga cgagcgccgg gatgtgagta ttcgcaggcc ccggccctct
    20521 atccgcaagc agctatccga cagcaaacag gccgccagcc caaaaaaggc ggcggccaga
    20581 accaaaaaga atgaattgga ggtataagga atgatgcact ttaccgtgga ggaagaaaac
    20641 ctgatctgta tgtaccacaa tgcagaccgg cgcagaacga taaacaggct caccgccgtc
    20701 atgccggtta tggatgggga tatgcgggcg caggcccagc agaccctttc caagctggaa
    20761 cgcatgacgg atgccgacta tgacggccag aagttccact tcacagacga ataacgaaca
    20821 gcagacgccg ggcgcggcgg ccatgtggtc gcttgcgtcc ggcgtctttt ctttttgcct
    20881 attcctacgc atttagaacc cggtattgtc ggggatagga tgaagtattc gcttgcccct
    20941 gcaaacatgc gggagaagcc ttgcagcggg cggttttcaa tcccttaaat gcgtaggttg
    21001 cagctataat tagacccctg aaacagatat agccgcgcag cgggtgtatc tgtttcaggg
    21061 gtgtgcaggt gatagccgca aagcggcgta agagggtgca gccctcttgc cgtctatctc
    21121 tgttccagcg ggaagctcgg caatgcttcc agcagtttca gggaaatgcg ctgccgtata
    21181 tcttcgtcaa tgactttctt ctttttgccg tttgcctgtc tgatctccac cgtggaaagc
    21241 tcgttgatgt agtcagcata atgggccaac actttttccg tggcccacct ctcaccggca
    21301 acggcggctt tgatcgtttc atagtccagc agcttttctt gtccgtccat cctgataccc
    21361 ccgtcagact gattttttct atgataccat attcggaagc atggaaccag agaaaacggc
    21421 gcttttctgc tggtaaattc tacgcattta gaacagcact tcggcgggga taggataaaa
    21481 cacccacata ccccgctgac catgccggaa aagccttgaa atataagggc ttttacagct
    21541 ctaagtgcgt aggtttccag tatcttttcc gctggcgctc ggcctctttg cggcggcgca
    21601 tccgtttgga gcaggccgcg cagtatttgg cccggttaga cttcggcgtg aacgtggccc
    21661 cgcactcaca gcagcgtttc acttcgtccc ggtctttggt gatctcggca tacagggccg
    21721 catccagcgg gaggacagcg gccttaaacc agcggcacag aagcgaatag gaaatgctct
    21781 gtacgcaaat acattcttcg ccgtcgtcca gcaacaggca gttgccgtca caatagttgc
    21841 agcactcatg cgtcagcttc cgggccttgc gatattgagg gtaattcatt cgtggaatac
    21901 tcatagctct tgcgtccatt cctttcccgg cgtcatacgc aaaatgccgt ccacattctg
    21961 cttgatgatg ccgtattcct gcatctgctt tttcgcttcg ccgtacttct catacagccg
    22021 ttccttttcc tcggccaatc gcttgtactc ggctttcagg gcgtacagat cggggagctt
    22081 tttcacaccc tgtcccttta gggcctttgc cgcagcttca tacaaaatca acgtgctttc
    22141 atgctcacgc cggaattttg ccttatccgc tgcattgcgg tattccatag ccacgggcca
    22201 gagctgcttg taggtagaaa tattctttat cagcaccgcc atatccgcaa gccggtgttc
    22261 catagctttg agggtagcgg cggcctcgtc gctggcggcg gtgatctcgg ccactttgct
    22321 ttccaaatcc gggtaggtgt ctatcccatg ttcagtcagg aaattcaaag tctttgcggc
    22381 ctgcttcaaa ttgtggagct tcgcccacct ctcatagcct gccgactgct gggccttaat
    22441 gttgttttcc agatcaatcc gcaaagaaat ccccttgcgc tctttgggcg ctctggtgat
    22501 aacgcggcct ttgatacgct ctttgatggc ttcctcggtg taggcttccc ccaatgtctt
    22561 gcagcgggta aagcgttcct gtccgggagc gcggaaagaa ataaactttc ccggcttgat
    22621 ctcatagccc tgcgcctgca tgagccgcag gaaatcatca aaatcttttg cctgcgggat
    22681 agcggcgtca atggctgctt tgagcttcgc tttccagctc gtccctttcc gctgggcgtc
    22741 ccactcggca tagctcttgc ccttactctg gccgggcttc accacggaca atccgttttc
    22801 cttgcacagc ctgtcgctgg tgcgccggat ataggcatag ctctgtttgt tggaaatgta
    22861 cttgcgctga tttaccatat ccacggcaca gaaaataatg tgattgtgga cgtggccctt
    22921 atcaatatgg gtagtcagca catagggata tttcccttgc agcacttcct cggcaagctg
    22981 cttcccgatc tcatgggcct gttccggcgt ggcttcgccg ggggcaaagg attgtatcag
    23041 gtggtggccc agattattgc ccttttggta tgcctgcgcc agcagcattt caaattcaat
    23101 gtccgctgtt tcataagagc aggcaaagga ggccaccagc agcttgccgt cagttttgtc
    23161 cgggttctcg atgtagtcaa gggctttttt cagcgttgac ttgataggat gtatctttgt
    23221 aactgccata tctgcgccag cgcccccttt atatcctcga tgtcctgtgc gtacaccggc
    23281 cccataccgt tcagcctctt tgcaatctga ttgacgttga cgccgatctt ctgtatctcg
    23341 gcggtctgcg cccggatgtc gctggtgtcc atgttgacga tatagccgtc aatgagcatc
    23401 ttccgggcgt aggctgaaaa gttcttcgta tgcagcagcg ccattttctg ttcaatcata
    23461 gcccgttcct ccggcgtcac agggacgcgc agcacgattt ttcgctttct gtttgccatg
    23521 cttcaccgtc ctttccattc aggggtttgg ggatttcccc aacaagcaaa acggacgcgg
    23581 cccggcaggg acggggcagg ccgtttttcc gggagtggct acactcccta tgcttgctac
    23641 gttactgttt ctccactaac aagacgtttc caaagcccca aactacccgc aaaatagccc
    23701 tctggaaaga aaaaagtgca aaaaaataga agctacgctg tgcagctccc gctaaaagta
    23761 aaacagagga tgccagttac acatcctccg tttcattctt cttcggcttt tcaattagta
    23821 tggcgctgtc aatcgcgccc tcgatgatcg gcaggtattc ttcggggcag agtttcagct
    23881 tgtggctgac cctttgccgc agttcgcttt cctcccgcat gatctccggg ttaaagtagc
    23941 gttccacagg aagcccgcag gctttaataa gctgtatgac aaccggcaga ctgggaatgg
    24001 cccgtcgttt tcgatgttgg caagatagcg ccagtcaata tttattttct ctgccattgc
    24061 tttgcgcgaa acgcgctttg cttgccgggc cgctttcaca tccgcgccga aagtttcaaa
    24121 gccgggacaa tcttcaatat tcgccatatc gcacctccca cttacattgt atatttcata
    24181 ctctgcccgt ggaatgttgg tatatgcacc tttcttaaag tttatatttc acattttgtt
    24241 tcttttattc ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac tatggactac
    24301 atgacgttga aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat aaactactat
    24361 tgtgctgcgg gacgcatccc cggcgctgta aagatggcga cgatctggct cattcccaaa
    24421 actgcggaaa agccgattga caggcgtaca aagaaaggaa aggcggttac tgaatagtat
    24481 aatgaaaccg gcgcattttg gatatggttt tgaagagaga gaaagcatgg tattttgtca
    24541 cggaaaaact ataagatgat agatggcagg ctgctccaga ccgataaaaa gtattctcat
    24601 ctgaaacaga aagaaaagat ttacacatgg atgtatgaag aagcgaaact ttatcatgac
    24661 agaaatggaa aatgcccgaa acaaagacga ggaagctgct gctgtatcag ccgtatatgg
    24721 tagaattgaa aaggccggca tctggattct ttatggtgaa gtctccagac attggataac
    24781 tgctggtcta atgcgccttt gagtatgggg tgattgattt taataccggg gaggtacggc
    24841 tttttgagga aaatattctg ggatttacat ttggcgatta ttacatttcc tgtaactgca
    24901 aggacacgga tgatgaagat atgaggaata tagacttgct gattttctac tgtcttgaac
    24961 ggtattagga ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg gagtacacac
    25021 aggccctgcc tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca
    25081 ttttgctgag gcttggggtg tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt
    25141 ggtggatcag aatatccgta aaggatattc tgatccattg cttgccagta ttatcgggat
    25201 gttttgacaa ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta attattatta
    25261 tcttgattta attcgtttaa accaataata tagatacata caccatgaaa agaagcgagt
    25321 atcccaatga cggatgatta aaatccgaaa agaaatcgct ttgaaactac agggaaacta
    25381 cagactgtta tgttatcttc ttaaaatgga gggattttta tgtcgatacg aattctactt
    25441 gtcgaggatg atgatcatat ctgtaataca gtaagggcgt ttctggctga ggcaggatat
    25501 caggtggatg cctgcacaga tggaaatgag gcatacacca agttttacga aaacacttat
    25561 caactggtta ttcttgatat tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa
    25621 tttcgtgcga aaaatgatac tcccattctg atgatgacag ccctgtcgga tgacgaaaac
    25681 caaatccggg cgtttgatgc agaggcagac gactatgtaa caaagccatt caagatgcag
    25741 attttactaa agcgggtgga agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc
    25801 cgtgtcggca ggctgacact tctgccggag gatttcacgg tactttgtga cggtacagag
    25861 ctgcccctga cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa caaaggcaga
    25921 accttaacgc atgaaatcat tttgtcacgt atatggggat atgactttga gggtgatggc
    25981 agcacagtcc acactcatat caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa
    26041 accattcgcg gtgtaggata ccgattggag gaatcataat aatggaaaga aaagggattt
    26101 tcattaaggt tttttcctat acgatcattg tcctgctact gcttgtcggt gtaacagcaa
    26161 cactgtttgc acagcaattt gtgtcttatt tcagagtgat ggaattacag caaacagtaa
    26221 aatcctatca gccattggtg gaactgattc agaatagcga taggcttgat attcaagagg
    26281 tggcagggct gtttcactac aataaccaat cctttgagtt ttatattgaa gataaagagg
    26341 gaagcgtact ctatgccaca ccaaatgcca atacatcaaa tagttttaga cccaactttc
    26401 tttatgtggt acatagagat gataatattt cgattgttgc tcaaagcaag gcaggtgtgg
    26461 gattgcttta tcaagggctg acaattcggg gaattgttat gattgcgata atggttgtat
    26521 tcagcctttt atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc aaagccttag
    26581 cggacagtgc gaataaaatg gcaaacctga aagatgtacc gccgccgctg gagcgaaagg
    26641 atgagcttgg cgcactggct catgatatgc attccatgta tgtcaggctg aaagaaacca
    26701 tcgcaaggct ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt
    26761 tctttgcggc agcttctcat gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg
    26821 agggaatgct tgaaaatatc ggtgactaca aagatcattc taagtatctg cgcgaatgca
    26881 tcaaaatgat ggataggcag ggcaaaatca tttccgaaat actggagctt gtcagcctga
    26941 atgatgggag aatcgtaccc atagctgaac cgttggacat agggcgcacg gttgccgagt
    27001 tgctgcccga ttttcaaacc ttggcagagg caaacaacca gcggttcgtc acagatattc
    27061 cagccgggca aattgtcctg tccgatccga ggctgctcca aaaggcacta tccaatgtca
    27121 tattgaatgc agttcagaac acgccgcagg gaggcgaggt acggatatgg agtgagcctg
    27181 gtgctgaaaa atgccgcctt tttgttttga acatgggcgt tcacattgat gatactgcgc
    27241 ttccaaggct gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc
    27301 gaagcagaaa aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg
    27361 ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt
    27421 taccgctcac atcaacattg taaatattta aaacttaaat gatttccctg ccggtctttt
    27481 tttgctgcta caggaaaact acagactggc tacagggaaa gtacagatgc gcttgccata
    27541 ataacaatcg taccagccac aaatcgtagt tttattgcaa aggaggcatt cgatcaaatg
    27601 gaaaaaagca actatcattc caatgcggat catcacaaac ggcatatgaa acaatccgtg
    27661 gaaaagcggg cttttctatg tgcgttcatt atttcgttca cagtctgcac gctgtttttg
    27721 gggtggagac tggcttccgt attggaggca acacagatac cgcccatccc tgcaactcat
    27781 acaggcagca gcactgacgt agtggagaat ttggaggaaa acgctcttgc caccgccaaa
    27841 gaacagggag atgaacagga atggagcctg attttagtga acaggcagaa ccccatcccc
    27901 gcacagtacg atgtggagct tgagcaacta tcaaatggtg agcggataga tattcggatt
    27961 tctccctatc ttcaagattt gtttgatgcc gcaagaactg atggagttta cccgattgtc
    28021 gcatccggat accgaacaac agaaaaacag caagaaatta tggatgaaaa aattgccgaa
    28081 tataaggcga aaggctacac ctctgcacag gctaaagcgg aagcagaaac ttgggtggcc
    28141 gtgccgggaa cgagcgagca tcagcttggt cttgctgtgg atatcaatgc ggacggaatt
    28201 cattcaacag gcaacgaggt ttatagatgg ctggatgaaa acagctatcg ttttggtttt
    28261 attcgtcgct acccaccaga caagacagag ataaccggtg tgagcaacga gccgtggcat
    28321 taccgatatg ttggcatcga agctgccaca gagatgtaca accaaggggt ttgccttgag
    28381 gaatatttaa aaccagaaaa atgagaaaag gatgttatgt tatggacaga aaaagattga
    28441 cacagcgttt cccgttcctg cttccaatga gacgggcgca gagaaaaatg tgcttttatg
    28501 cgggaatgag atttgacggc tgtcgctatg cgcagacgat aggggaaaaa tcgctttccc
    28561 atttgctctt tgaaacggat tgcgcattat acaaccacaa taccggattt gacatgatat
    28621 accaggaaaa caaggtgttc aacttaaagc tggcagcaaa gaccttaaac ggcttattga
    28681 taagaccggg ggaaaccttt tctttctggt tgttcctgca tacgccattg acaattatcc
    28741 agcgcagagg tcacgaagta aaggagtttc cagagccaaa cagtgacgag attaaagggg
    28801 tggatgcaac catttcagag ggctggattg atttaaaagt gcgaaacgat accgactgca
    28861 cctaccaaat atgggtgacc ttagatgatg agaaaatcat cggtcaggtg ttcgccgaca
    28921 aagagcccca agcattatac aaaattacaa atggcagtat ccagtatgtc cgtgaaagtg
    28981 gcgggattta tgaatatgcc caggttaaac ggatgcaagt tgccttaggt accggggaaa
    29041 taatagattg caagctgctt tatacaaaca aatgcaaaat ctgttatccc ctcccggaaa
    29101 gtgtggatat tcaggaggag aaccaatgag aaaaagtgtg ggcattactg tttttggatg
    29161 cgaacaggat gaggcaaatg ctttccgcgc tttatcgccg gattttcata ttatccctac
    29221 gctgattagc gatgcgatat cggcagacaa cgcaaaattg gccgctggca atcaatgcgt
    29281 tagcgtaagc cataagtccg aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg
    29341 ggtaaaatac atttctaccc gcagcatcgg ctgcgatcac attgatacga ctgccgccga
    29401 gagaatggga atctcggttg gcacggttgc gtattcgccg gacagcgttg cggattatgc
    29461 tttgatgctg atgctgatgg ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca
    29521 acaagatttc agattggatc gtatccgggg gaaagaactg ggggatatga ctgtgggagt
    29581 tattggaacc ggccatatcg ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca
    29641 tgtgctggcc tatgataaca gccgaaaaat ggatgcagat tatgtccagc ttgatgagct
    29701 tctaaaaaac agcgatattg ttacgctcca tgtgccgctt tgtgcggata cccgccatct
    29761 gatcggtcag aagcaaattg gagagatgaa gcaaggcgca tttttaatca acaccgggcg
    29821 cggagcgctt gtcgataccg gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg
    29881 tgcggcactg gatgtgttgg agggcgagga tcagtttgtt tataccgatt gctcgcagaa
    29941 agtgcttgac catccctttt tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc
    30001 ccatacggcg tactacactg agcgtgtgct gcaggatact acagaaaaaa caatcaggaa
    30061 ttgtctcaat tttgaaagga gtttacagca tgaatagaat aaaagtcgca atcatcttcg
    30121 gcggttgctc ggaggaacat gatgtgtcgg taaaagccgc aatagaaatt gctgcgaaca
    30181 ttgatacgga aaaattcgat ccgcactaca tcggaattac aaaaaacggt gtatggaagc
    30241 tatgcaagaa gccatgtacg gaatgggaag ccgacagtct ccccgccata ctctccccgg
    30301 ataggaaaac gcatgggctg cttgtcatga aagaaagcga atacgaaaca cggcgtattg
    30361 atgtggcttt cccggttttg catggcaaat gcggggagga tggtgcgata caggggctgt
    30421 ttgtattgtc tggtatcccc tatgtgggct gtgatattca aagctccgca gcttgcatgg
    30481 acaaatcact ggcctacatt cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa
    30541 tgattgataa aggtgacaag ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc
    30601 cggcacggtc aggttcgtcc tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg
    30661 ctgcgataga agcggcagga caatatgatg gaaaaatctt aattgagcaa gcgatttcgg
    30721 gctgtgaggt cgggtgtgcg gtcatgggga acgaggatga tttgattgtc ggcgaagtgg
    30781 atcaaatccg gctgagccac ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag
    30841 gctcagaaaa tgcgatgatt acagttcccg cagacattcc ggtcgaggaa cgaaatcggg
    30901 tgcaggaaac ggcaaagaaa gtatatcggg tgcttggatg cagagggctt gcccgtgttg
    30961 atcttttttt gcaggaggat ggcggcatcg ttctaaatga ggtcaatacc ctgcccggtt
    31021 ttacatcgta cagccgctac ccacgtatga tggccgccgc aggaatcacg cttcctgcac
    31081 tgattgacag cctgattaca ttggcgttaa agaggtgata agtatggaaa atggtttttt
    31141 gtttttagat gaaatgttgc atggcgtccg ttgggatgcc aagtacgcca catgggataa
    31201 cttcacagga aaaccggtgg atgggtatga ggtgaatcgc atcatcggca caaaggccgt
    31261 ggcgtttgct ctgcgcgagg cacaaatcca tgcggctgcg cttggctatg gcttgctttt
    31321 atgggatgga tatcggccaa gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc
    31381 ggaggacaat ctcacaaaag aaaaatttta ccccaatata gagcgagccg agttgattac
    31441 aaagggttat gtggcctcac aatccagcca tagccgtgga agcgcaattg atcttacgct
    31501 ctaccacctg gatacagggg aacttgtttc aatgggaagt aacttcgatt ttatggacga
    31561 acggtcgcat catacagcaa aagggatagg ggatgcagag gcacaaaatc gaagatgctt
    31621 gcgtaaaatc atggaaagca gcggatttca gtcttatcgc tttgaatggt ggcactataa
    31681 gttgattgat gagccatacc ccgataccta ttttaatttt gccgtttcat aatgaaagta
    31741 ttggttttgt atcagatttt ggatttgata aaatctatgc tggtcatgga caatctttta
    31801 ctggaaagtg gttaaagcat cttttgtaat acattgcaaa ttatagtatt caatctgccc
    31861 agcggcagaa aagaaaaaaa ccgctgttgg gcagaccttt cccacgccca ttttgtattt
    31921 tgctggcggc ggttttgaga aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg
    31981 cggcccatag gaggatgccg ctatgctgtt ttgctattgc catgctctaa tccacttcca
    32041 ccactcaaaa aaagccttgc ccctc
//
LOCUS       DAYYAG010000019        31905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000019 DAYYAG010000000
VERSION     DAYYAG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.019"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACHARQ_000768"
     CDS             3..371
                     /locus_tag="ACHARQ_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8409217.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACHARQ_000769"
     CDS             551..1492
                     /locus_tag="ACHARQ_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8409218.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACHARQ_000770"
     CDS             1561..2292
                     /locus_tag="ACHARQ_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8409219.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACHARQ_000771"
     CDS             2436..4616
                     /locus_tag="ACHARQ_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8409220.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACHARQ_000772"
     CDS             4636..5118
                     /locus_tag="ACHARQ_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8409221.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACHARQ_000773"
     CDS             5134..5895
                     /locus_tag="ACHARQ_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8409222.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACHARQ_000774"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACHARQ_000774"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8409223.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACHARQ_000775"
     CDS             7667..8152
                     /locus_tag="ACHARQ_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8409224.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACHARQ_000776"
     CDS             8145..8828
                     /locus_tag="ACHARQ_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8409225.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACHARQ_000777"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACHARQ_000777"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8409226.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACHARQ_000778"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACHARQ_000778"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8409227.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACHARQ_000779"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACHARQ_000779"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8409228.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACHARQ_000780"
     CDS             13125..13457
                     /locus_tag="ACHARQ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8409229.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHARQ_000781"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHARQ_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8409230.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACHARQ_000782"
     CDS             14283..15656
                     /locus_tag="ACHARQ_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8409231.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHARQ_000783"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHARQ_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8409232.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACHARQ_000784"
     CDS             16711..17151
                     /locus_tag="ACHARQ_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8409233.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACHARQ_000785"
     CDS             17295..17996
                     /locus_tag="ACHARQ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8409234.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACHARQ_000786"
     CDS             17993..19114
                     /locus_tag="ACHARQ_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8409235.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACHARQ_000787"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACHARQ_000787"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8409236.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACHARQ_000788"
     CDS             20493..21401
                     /locus_tag="ACHARQ_000788"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8409237.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACHARQ_000789"
     CDS             21436..21708
                     /locus_tag="ACHARQ_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8409238.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACHARQ_000790"
     CDS             21719..22765
                     /locus_tag="ACHARQ_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8409239.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACHARQ_000791"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACHARQ_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8409240.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACHARQ_000792"
     CDS             complement(25776..26462)
                     /locus_tag="ACHARQ_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409241.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACHARQ_000793"
     CDS             complement(26440..26934)
                     /locus_tag="ACHARQ_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8409242.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACHARQ_000794"
     CDS             complement(26954..28174)
                     /locus_tag="ACHARQ_000794"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8409243.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACHARQ_000795"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACHARQ_000795"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8409244.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="ACHARQ_000796"
     CDS             30352..31263
                     /locus_tag="ACHARQ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409245.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        10776 a         5570 c         6854 g         8705 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaat
//
LOCUS       DAYYAG010000020        31262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000020 DAYYAG010000000
VERSION     DAYYAG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.020"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..525)
                     /gene="yajC"
                     /locus_tag="ACHARQ_000797"
     CDS             complement(145..525)
                     /gene="yajC"
                     /locus_tag="ACHARQ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8409246.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(622..1767)
                     /gene="tgt"
                     /locus_tag="ACHARQ_000798"
     CDS             complement(622..1767)
                     /gene="tgt"
                     /locus_tag="ACHARQ_000798"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8409247.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1985..2683
                     /locus_tag="ACHARQ_000799"
     CDS             1985..2683
                     /locus_tag="ACHARQ_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8409248.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2851..5490)
                     /locus_tag="ACHARQ_000800"
     CDS             complement(2851..5490)
                     /locus_tag="ACHARQ_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8409249.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6109..7140)
                     /gene="queA"
                     /locus_tag="ACHARQ_000801"
     CDS             complement(6109..7140)
                     /gene="queA"
                     /locus_tag="ACHARQ_000801"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8409250.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7333..8508
                     /locus_tag="ACHARQ_000802"
     CDS             7333..8508
                     /locus_tag="ACHARQ_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8409251.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8512..9153
                     /locus_tag="ACHARQ_000803"
     CDS             8512..9153
                     /locus_tag="ACHARQ_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409252.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9150..10067
                     /locus_tag="ACHARQ_000804"
     CDS             9150..10067
                     /locus_tag="ACHARQ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8409253.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10064..10735
                     /locus_tag="ACHARQ_000805"
     CDS             10064..10735
                     /locus_tag="ACHARQ_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409254.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11276..11764)
                     /locus_tag="ACHARQ_000806"
     CDS             complement(11276..11764)
                     /locus_tag="ACHARQ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8409255.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11965..12231)
                     /locus_tag="ACHARQ_000807"
     CDS             complement(11965..12231)
                     /locus_tag="ACHARQ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8409256.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12231..12494)
                     /locus_tag="ACHARQ_000808"
     CDS             complement(12231..12494)
                     /locus_tag="ACHARQ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8409257.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13015..13155
                     /locus_tag="ACHARQ_000809"
                     /pseudo
     CDS             13015..13155
                     /locus_tag="ACHARQ_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13297..15120)
                     /locus_tag="ACHARQ_000810"
     CDS             complement(13297..15120)
                     /locus_tag="ACHARQ_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8409258.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15718..18423)
                     /locus_tag="ACHARQ_000811"
     CDS             complement(15718..18423)
                     /locus_tag="ACHARQ_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8409259.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18729..19676)
                     /locus_tag="ACHARQ_000812"
     CDS             complement(18729..19676)
                     /locus_tag="ACHARQ_000812"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8409260.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19866..20135
                     /gene="rpsN"
                     /locus_tag="ACHARQ_000813"
     CDS             19866..20135
                     /gene="rpsN"
                     /locus_tag="ACHARQ_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8409261.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20311..20673)
                     /locus_tag="ACHARQ_000814"
     CDS             complement(20311..20673)
                     /locus_tag="ACHARQ_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8409262.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20684..21805)
                     /gene="alr"
                     /locus_tag="ACHARQ_000815"
     CDS             complement(20684..21805)
                     /gene="alr"
                     /locus_tag="ACHARQ_000815"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8409263.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21821..22171)
                     /gene="acpS"
                     /locus_tag="ACHARQ_000816"
     CDS             complement(21821..22171)
                     /gene="acpS"
                     /locus_tag="ACHARQ_000816"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8409264.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22301..23812)
                     /locus_tag="ACHARQ_000817"
     CDS             complement(22301..23812)
                     /locus_tag="ACHARQ_000817"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8409265.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24134..25486)
                     /gene="murF"
                     /locus_tag="ACHARQ_000818"
     CDS             complement(24134..25486)
                     /gene="murF"
                     /locus_tag="ACHARQ_000818"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8409266.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25639..26715)
                     /locus_tag="ACHARQ_000819"
     CDS             complement(25639..26715)
                     /locus_tag="ACHARQ_000819"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8409267.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26911..28656
                     /locus_tag="ACHARQ_000820"
     CDS             26911..28656
                     /locus_tag="ACHARQ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8409268.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28936..29373)
                     /locus_tag="ACHARQ_000821"
     CDS             complement(28936..29373)
                     /locus_tag="ACHARQ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8409269.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29357..30730)
                     /locus_tag="ACHARQ_000822"
     CDS             complement(29357..30730)
                     /locus_tag="ACHARQ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409270.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31052..>31262)
                     /locus_tag="ACHARQ_000823"
     CDS             complement(31052..>31262)
                     /locus_tag="ACHARQ_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8409271.1"
                     /translation="QGLTSSVFRWYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFE
                     ITEELISDIGQGFQMVDYKMKKALA"
BASE COUNT         8900 a         6670 c         5809 g         9883 t
ORIGIN      
        1 aagttattta ttattattta tatgacacac ccgattattc aatattgagc aatatatccc
       61 atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt ctgccctacc
      121 tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct cttccagcgt
      181 ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg tttcaacagt
      241 agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg ctgcacgatc
      301 gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat tgtcgatttc
      361 tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt tcatgctatc
      421 taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa aaaaccacat
      481 tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt tcacctcact
      541 aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta gttttttact
      601 taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga acccgtattc
      661 ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg
      721 tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt
      781 ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca
      841 atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag
      901 acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc
      961 aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat
     1021 caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat
     1081 ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc
     1141 gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc
     1201 aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga
     1261 cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc
     1321 aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga
     1381 taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt
     1441 ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc
     1501 ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag
     1561 ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg
     1621 ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt
     1681 tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa
     1741 acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga
     1801 caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta
     1861 ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat
     1921 tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa
     1981 aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg
     2041 gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct
     2101 tattatgatt cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc
     2161 agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat
     2221 cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca
     2281 aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg
     2341 cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt
     2401 agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga
     2461 tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa
     2521 attgatggat ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca
     2581 tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc
     2641 aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt
     2701 gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc
     2761 cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag
     2821 taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg
     2881 atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa
     2941 ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc
     3001 aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc
     3061 taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga
     3121 ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg
     3181 gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac
     3241 ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg
     3301 aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg
     3361 aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca
     3421 tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa
     3481 gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat
     3541 cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc
     3601 tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc
     3661 atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt
     3721 gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc
     3781 gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc
     3841 taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt
     3901 gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat
     3961 cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg
     4021 cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa
     4081 gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac
     4141 atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat
     4201 catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc
     4261 tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc
     4321 tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat
     4381 tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc
     4441 tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga
     4501 gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg
     4561 tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt
     4621 ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc
     4681 tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat
     4741 tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt
     4801 caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt
     4861 cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga
     4921 ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa
     4981 cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc
     5041 atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt
     5101 cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag
     5161 tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc
     5221 gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga
     5281 aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt
     5341 tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg
     5401 ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac
     5461 ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa
     5521 aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa agggcttatc
     5581 gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg
     5641 atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga
     5701 tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta
     5761 gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac
     5821 aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac
     5881 aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa
     5941 attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat
     6001 ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa
     6061 tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa
     6121 catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga
     6181 taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa
     6241 atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat
     6301 atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa
     6361 tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt
     6421 caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc
     6481 aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac
     6541 taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc
     6601 tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc
     6661 ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc
     6721 tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt
     6781 cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt
     6841 cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc
     6901 taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt
     6961 attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc
     7021 ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa
     7081 tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat
     7141 atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc
     7201 aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt
     7261 gtattttttc attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa
     7321 agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc
     7381 gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg
     7441 agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa
     7501 gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt
     7561 cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat
     7621 atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa
     7681 aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta
     7741 tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc
     7801 atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac
     7861 cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg
     7921 gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg tgtgatccaa
     7981 tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga agagttgatt
     8041 ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga agttatgcta
     8101 aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa actgatgagg
     8161 gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa aggatttatc
     8221 gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga cattttgaat
     8281 aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca acgcatttta
     8341 aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg
     8401 actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga tcccgcaact
     8461 actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc tatgcagcag
     8521 ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct ttatatttca
     8581 ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt actgacacgt
     8641 tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac aatcccttct
     8701 cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc tgcgattatc
     8761 gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg gatgaaaaat
     8821 gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt
     8881 ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg tttagctgcc
     8941 gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg tttaggcgat
     9001 ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg tacgatccct
     9061 gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca ccggctgaca
     9121 ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg accatggttc
     9181 ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc aatacagatg
     9241 aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta gcaagtctag
     9301 ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac aatctggcta
     9361 ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca gcggctcgct
     9421 acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat ccgaaaaaag
     9481 cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct
     9541 atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa aaataccatc
     9601 tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt gtagatactt
     9661 cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat ggcttctcct
     9721 tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa
     9781 tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat ctagtcatgt
     9841 tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta gacaacaaga
     9901 tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat accatcagta
     9961 cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa ccatcagtag
    10021 tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc aattggataa
    10081 aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct atatctttgc
    10141 acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag cagttgtcgg
    10201 gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg tgattcgttt
    10261 ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa tcattggatt
    10321 aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct taccgatcat
    10381 caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg tcggcaaagg
    10441 catggggatg actgctagac aacgactatt catggtagag ttaccactct ctgtttctgt
    10501 tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg cgatcggtgc
    10561 attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg caacagaggg
    10621 cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca tcacggactg
    10681 gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac gctgatgtta
    10741 ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt
    10801 catttttaag gcaataatcc actctgattc ttccctcctt atgggcaaag agttgctttt
    10861 cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt tcatatctgc
    10921 atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca ttttcccatc
    10981 actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt tctttgaatt
    11041 gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc atcagtcctc
    11101 gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc ggtaacttcg
    11161 atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa catatctagc
    11221 cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac gattcttatg
    11281 gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga agagcacccc
    11341 cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa aagatatcga
    11401 tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc acgactttaa
    11461 cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta
    11521 aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac catgccaacg
    11581 aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt ttcaacatac
    11641 ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt tggcgataat
    11701 cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg attttccttg
    11761 tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct aaactcaaac
    11821 aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca ataaatccaa
    11881 gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt tgttaactat
    11941 ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat aaatttctac
    12001 aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca gcctgcgtga
    12061 gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca tatatggatc
    12121 ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt
    12181 tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca tcaaagagct
    12241 tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact
    12301 ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc
    12361 agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat ttcagtatga
    12421 ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct aggattcatc
    12481 gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta cttgattaag
    12541 tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct tttttacata
    12601 gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta ctcctttacg
    12661 actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag caaaggtcga
    12721 atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt
    12781 gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta acggttattc
    12841 aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct ctttcgtctc
    12901 ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt tgtgaatcac
    12961 caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga ggctgagatt
    13021 cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata
    13081 ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat caaaatcaaa
    13141 atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc atctttaatg
    13201 gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag cttcaattaa
    13261 gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat ggtagctata
    13321 ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca aacttgtttg
    13381 gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca acacagtagt
    13441 tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga taattggatt
    13501 ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga aatgcttttt
    13561 aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa catgcatcgc
    13621 aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta cccgtgtcac
    13681 attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg catcattttc
    13741 taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa aaatgaacat
    13801 aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga caaacaaaat
    13861 cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca
    13921 tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag caaatggaat
    13981 gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga aaataaacag
    14041 caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta gtcgatcccc
    14101 acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg caagaactgg
    14161 aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag caaattgaag
    14221 gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca aaacagtgac
    14281 ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca cgaagaagaa
    14341 acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc ttggcttctt
    14401 aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag aaccagaaga
    14461 aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg gaagtgcagt
    14521 agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga tcagtaacga
    14581 gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt ccctcagccc
    14641 acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga cttgatgacc
    14701 gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag atactgctac
    14761 tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat tctttccaag
    14821 attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga tgatttgacg
    14881 atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg gtagcgaata
    14941 ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg taccatatgc
    15001 aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg ttaatttgtg
    15061 ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta agtaatccaa
    15121 tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa tttatagtaa
    15181 acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag aaaataattg
    15241 gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca aaaatctaac
    15301 atttttttat gggctttttc tttgaattct ataataaaag gatagaactt gacttagaaa
    15361 atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag gctgctttct
    15421 acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg ataaacggtg
    15481 tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa gaagctgttt
    15541 ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc ttggtacacg
    15601 gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat gaggaactat
    15661 ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc tcttttttta
    15721 atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt cttcgatgaa
    15781 cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca tgatccagat
    15841 tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca agtttaccgc
    15901 atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga cttggatcgg
    15961 atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga tccctttacc
    16021 gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta aggcgatcaa
    16081 gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta agaaaatatt
    16141 ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca gcatacgagc
    16201 aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat agtattctgt
    16261 cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag tataaaacgg
    16321 ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta gctcaacagg
    16381 ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga tgacttcgcc
    16441 tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga taccagcttt
    16501 gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg tttcgacttc
    16561 tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta gtgctggcca
    16621 atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg gtaattcatt
    16681 gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat ttccattcag
    16741 cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt gatatttttc
    16801 taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg aatcaaagtc
    16861 gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga tgacttggtc
    16921 tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa tgatgccatt
    16981 gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct tcgtgtcttc
    17041 attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta gcaagggttt
    17101 taattgattt agctgatcaa taattggctg taatttcgta tttccagcac ttgtttgcaa
    17161 tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata ttccgtcaat
    17221 aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct taccatcttc
    17281 aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt gtccaattga
    17341 cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca gctgagaggc
    17401 acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt tcacatctgg
    17461 caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat cgattccttg
    17521 gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt gcttaccggc
    17581 attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt gatctttcaa
    17641 ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta ctttagggat
    17701 gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta gttgactttg
    17761 taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat ctgttgataa
    17821 aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt aacctatctc
    17881 attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg tgatttgcga
    17941 ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga ttttttcatt
    18001 taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca gcagatcttc
    18061 tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga ctccgtcttc
    18121 taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat gaagcgattc
    18181 gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct tgtctgcact
    18241 gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg ctttgatgtt
    18301 aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg ttgcaatcgg
    18361 gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt tgattgactg
    18421 catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat ttcatacttt
    18481 tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca attgtctcat
    18541 tgtaatctct tacatattga gaaataatta aggattacag cctttacttt atcatgaaat
    18601 aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac gcaaaaaagc
    18661 taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt tttttattcc
    18721 tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg tccttcaact
    18781 agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga aataaaaaca
    18841 tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc atctagatat
    18901 tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa atctagatct
    18961 gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat gccggtttct
    19021 cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc aaattgcaga
    19081 cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt taccttttcc
    19141 tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag aagctggatc
    19201 agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt cggctggatt
    19261 tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat tccggctgct
    19321 tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa cacgacttct
    19381 tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg aacagttgtg
    19441 acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac ataagggaat
    19501 ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata aggattcaca
    19561 ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg atgagcatcg
    19621 atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc
    19681 accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa aaaaatgtct
    19741 tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa
    19801 ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat tggaggaatt
    19861 tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa acgattgaac
    19921 gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac
    19981 ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat ggacgccctc
    20041 gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta gcacataaag
    20101 gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt aatgtaaagg
    20161 aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct tcttctagca
    20221 gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata
    20281 tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat
    20341 acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca
    20401 gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga
    20461 gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt
    20521 agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt
    20581 attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag
    20641 atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct tgatattctc
    20701 ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg
    20761 ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc
    20821 cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg
    20881 tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat
    20941 ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc
    21001 catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct
    21061 caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt
    21121 acattgctac tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg
    21181 cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa
    21241 agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg
    21301 taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta
    21361 cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg
    21421 aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc
    21481 gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa
    21541 ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat
    21601 ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa
    21661 tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa
    21721 gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg
    21781 ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt
    21841 gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg
    21901 aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa
    21961 cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc
    22021 ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg
    22081 tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt
    22141 cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa
    22201 cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa
    22261 tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga
    22321 ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat
    22381 ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac
    22441 ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg
    22501 ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta
    22561 gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca
    22621 ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta
    22681 attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg
    22741 tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca
    22801 taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt
    22861 gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa
    22921 cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc
    22981 gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc
    23041 cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg
    23101 tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat
    23161 agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat
    23221 ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta
    23281 atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca
    23341 tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca agtttcaatg
    23401 tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat
    23461 ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta
    23521 cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg
    23581 ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg
    23641 gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc
    23701 cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc
    23761 gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc
    23821 tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc
    23881 aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg
    23941 tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg
    24001 ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag
    24061 tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca
    24121 agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc
    24181 tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg atagcatcaa
    24241 tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca
    24301 attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag
    24361 gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca
    24421 tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata
    24481 ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc
    24541 ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta
    24601 tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta
    24661 cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt
    24721 gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg
    24781 tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa
    24841 cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt
    24901 ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg
    24961 gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa
    25021 cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt
    25081 tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat
    25141 ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt
    25201 ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg
    25261 gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg
    25321 tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac
    25381 ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc
    25441 attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta
    25501 aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca
    25561 agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag
    25621 cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg
    25681 attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc
    25741 ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag
    25801 gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa
    25861 catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc
    25921 ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc
    25981 tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc
    26041 aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata
    26101 ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact
    26161 agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc
    26221 aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg
    26281 cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact
    26341 ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt
    26401 tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat
    26461 ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca
    26521 tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac ctttgaccca
    26581 ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat aaatggcatt
    26641 taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc cgccatatag
    26701 taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga tttactctca
    26761 tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg agccagcttc
    26821 taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt tttttataaa
    26881 aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg cagccgcttg
    26941 gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt acagcaaaaa
    27001 aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat caagatagga
    27061 cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac attcaaaaca
    27121 ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat cgaaacagaa
    27181 ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt tgcctctgct
    27241 tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt cgttaagatc
    27301 caacacccta acatcgccga agagatccga cttgatcttc aactatttga acgagcgatc
    27361 cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt actccaagaa
    27421 gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa
    27481 ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta tgacgctttt
    27541 tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa tcatttaatg
    27601 aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca gctgaaacaa
    27661 gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga tggttttttc
    27721 catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga agaacagaca
    27781 caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt tgcttttcga
    27841 gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat cgattttggt
    27901 atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt tttagcgctg
    27961 tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc
    28021 tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat
    28081 tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac gatttgtcat
    28141 caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt tgggacatta
    28201 gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt
    28261 gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca aagcggatta
    28321 gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca ccatctatta
    28381 gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca agacaaatta
    28441 ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt agctgcattg
    28501 atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc
    28561 ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt ggcaatcgat
    28621 gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc
    28681 agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt gtgaaaattc
    28741 ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg
    28801 ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt atcctgtttc
    28861 cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc
    28921 tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag acatatgatc
    28981 tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact
    29041 atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg
    29101 atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat
    29161 caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg
    29221 gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact
    29281 ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac
    29341 tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat
    29401 aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca
    29461 gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg
    29521 gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca
    29581 cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg
    29641 tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa
    29701 gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca
    29761 gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga
    29821 ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt
    29881 ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca
    29941 ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt
    30001 gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa
    30061 aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata
    30121 tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca
    30181 gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa
    30241 tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa
    30301 atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct
    30361 tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca
    30421 aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac
    30481 cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta
    30541 gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa
    30601 tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa
    30661 ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt
    30721 tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag
    30781 cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa aatacagaaa
    30841 gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt
    30901 ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat
    30961 tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc
    31021 gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc attttataat
    31081 cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt
    31141 catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc
    31201 tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt
    31261 ga
//
LOCUS       DAYYAG010000021        29334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000021 DAYYAG010000000
VERSION     DAYYAG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.021"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..293)
                     /locus_tag="ACHARQ_000824"
     CDS             complement(<1..293)
                     /locus_tag="ACHARQ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8409272.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGAD"
     gene            complement(326..1411)
                     /locus_tag="ACHARQ_000825"
     CDS             complement(326..1411)
                     /locus_tag="ACHARQ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8409273.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            complement(1430..2470)
                     /locus_tag="ACHARQ_000826"
     CDS             complement(1430..2470)
                     /locus_tag="ACHARQ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8409274.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(2486..3877)
                     /locus_tag="ACHARQ_000827"
     CDS             complement(2486..3877)
                     /locus_tag="ACHARQ_000827"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8409275.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            4255..5238
                     /locus_tag="ACHARQ_000828"
     CDS             4255..5238
                     /locus_tag="ACHARQ_000828"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8409276.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(5606..7744)
                     /locus_tag="ACHARQ_000829"
     CDS             complement(5606..7744)
                     /locus_tag="ACHARQ_000829"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8409277.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(7783..9369)
                     /locus_tag="ACHARQ_000830"
     CDS             complement(7783..9369)
                     /locus_tag="ACHARQ_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8409278.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(9359..10579)
                     /locus_tag="ACHARQ_000831"
     CDS             complement(9359..10579)
                     /locus_tag="ACHARQ_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409279.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(10591..11397)
                     /locus_tag="ACHARQ_000832"
     CDS             complement(10591..11397)
                     /locus_tag="ACHARQ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409280.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(11607..12887)
                     /locus_tag="ACHARQ_000833"
     CDS             complement(11607..12887)
                     /locus_tag="ACHARQ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8409281.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(12931..14964)
                     /locus_tag="ACHARQ_000834"
     CDS             complement(12931..14964)
                     /locus_tag="ACHARQ_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409282.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(14998..15849)
                     /gene="rfbD"
                     /locus_tag="ACHARQ_000835"
     CDS             complement(14998..15849)
                     /gene="rfbD"
                     /locus_tag="ACHARQ_000835"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8409283.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(15947..16975)
                     /gene="rfbB"
                     /locus_tag="ACHARQ_000836"
     CDS             complement(15947..16975)
                     /gene="rfbB"
                     /locus_tag="ACHARQ_000836"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8409284.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(16997..17569)
                     /gene="rfbC"
                     /locus_tag="ACHARQ_000837"
     CDS             complement(16997..17569)
                     /gene="rfbC"
                     /locus_tag="ACHARQ_000837"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8409285.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(17583..18449)
                     /gene="rfbA"
                     /locus_tag="ACHARQ_000838"
     CDS             complement(17583..18449)
                     /gene="rfbA"
                     /locus_tag="ACHARQ_000838"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8409286.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(18549..19256)
                     /locus_tag="ACHARQ_000839"
     CDS             complement(18549..19256)
                     /locus_tag="ACHARQ_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8409287.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(19261..20088)
                     /locus_tag="ACHARQ_000840"
     CDS             complement(19261..20088)
                     /locus_tag="ACHARQ_000840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8409288.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(20088..20888)
                     /locus_tag="ACHARQ_000841"
     CDS             complement(20088..20888)
                     /locus_tag="ACHARQ_000841"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8409289.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(20881..22020)
                     /locus_tag="ACHARQ_000842"
     CDS             complement(20881..22020)
                     /locus_tag="ACHARQ_000842"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8409290.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(22225..23418)
                     /locus_tag="ACHARQ_000843"
     CDS             complement(22225..23418)
                     /locus_tag="ACHARQ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8409291.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(23497..23703)
                     /locus_tag="ACHARQ_000844"
     CDS             complement(23497..23703)
                     /locus_tag="ACHARQ_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8409292.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(23696..24838)
                     /locus_tag="ACHARQ_000845"
     CDS             complement(23696..24838)
                     /locus_tag="ACHARQ_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8409293.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            25350..25985
                     /locus_tag="ACHARQ_000846"
     CDS             25350..25985
                     /locus_tag="ACHARQ_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8409294.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            26121..26213
                     /locus_tag="ACHARQ_000847"
                     /pseudo
     CDS             26121..26213
                     /locus_tag="ACHARQ_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            26327..26962
                     /locus_tag="ACHARQ_000848"
     CDS             26327..26962
                     /locus_tag="ACHARQ_000848"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8409295.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            27069..28307
                     /gene="mef(H)"
                     /locus_tag="ACHARQ_000849"
                     /gene_synonym="mefH"
     CDS             27069..28307
                     /gene="mef(H)"
                     /locus_tag="ACHARQ_000849"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8409296.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            28534..28953
                     /locus_tag="ACHARQ_000850"
     CDS             28534..28953
                     /locus_tag="ACHARQ_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8409297.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            28958..29197
                     /locus_tag="ACHARQ_000851"
     CDS             28958..29197
                     /locus_tag="ACHARQ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409298.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         8982 a         5788 c         4960 g         9604 t
ORIGIN      
        1 taatcagcac cagataataa tacatatttt aaatatttct cattttgtaa tgctctcttt
       61 actaaattga tttctgcttg tacaatggaa aaaccacccc aataaatttt aacacggttt
      121 ttattacata aaaattcaac attatcatat gatttaaatg cttcaataaa tggttctata
      181 tcagtttttt tatctatatg aataataaaa actgcatttt cgttcaatcg attgaccaat
      241 ctctttaagt tgccaatatc attgtatgcc aatatcaaat aacatatttt caaaaataat
      301 cactcctaaa atatcttgaa catttttaat gatctctact atatatactc tccatttgtt
      361 cagtagtgta cgaaacatca taactcttta gttcttgtgt tattttatta gcatacggct
      421 cttttattac tttttttgcc cattcttgtg gtgtttcatc taatgataca aaatccacat
      481 tatttgttaa ctttgtatct ttggaaatag tatctgctaa gactaatttt aattttgctg
      541 cttgtgcttc taatccaacc attggcagac cttcataaag agaaggcatt acaaaaacat
      601 ccatcacaga taataactcg ggaatatcgt ttctttctcc taaaaatttt actcttttct
      661 ctatatccaa ttctactgct ttggctttca gtttatttct aagtgaccct tcacctacta
      721 aaatcaagta agaatcagaa ttcattttta gtatttctaa aaaaatctct aacaaataag
      781 attgattttt ttgtttctct aatcgtccaa tgtttcccac aacaaaagta ctcattgaga
      841 tatccaattc ttttctctta ttaaccttag cttctgaatt tttactataa agctctaaat
      901 caataccatt atgaactatc tcaaaggggt ggttttgaaa cagactttca ccagcttctt
      961 gactaacggc aaggtagtct gttgcatttt gtaacaacaa tttggttaaa aatttacgca
     1021 atacattcat tacaatattt tgagattttc catcttcagt tgcatggcta tgagcaattc
     1081 tctgtctgat tccattcttt ttggctaacc ataaattaat tcccccgcca aaataaacct
     1141 gattgtgaac tatagaaaaa gattcttttt ttagtaactt attcacttct attaaatatt
     1201 taaaaatatt tttcttagga ttaggtacaa caaaaatacg gcctcctaat ctttcaatct
     1261 ctgaatgata gtcttgaaca tctgcataat cttcataaat caaaaaatca aattgatagt
     1321 ttgtacgatc aattttacga tataaattca tcatgaaaga ctctgctcca cctaaaccca
     1381 ttcgtccctg aaaatgcaaa attcttttca ttatattttc ctccattaac taatatactc
     1441 gcttcaaagc agccattaac cctggcattt tcacttcaac gaagcttcca acttttacag
     1501 cagataaaaa tagtggagct ggcatattgt aaagcaactt tgttattttt tttgctgaag
     1561 aatccgtctt agcaatttgc ttggcttctc ggatcactgg atcactcaat atctgctttg
     1621 cttcgtcttt cttctctttt gaagttcttg cagaggtact ataaatagaa aaaataactg
     1681 aagttgtccc ttttaaaaga agcgccgcca taccgtcttt attaatatcg tctgttacag
     1741 tatttgctaa tgaatagaat tctttaaatg tttcgaactt gttaggtcgg tattgattag
     1801 tgatcccctt cccaccttca aattcataaa tatattctac ttttgggttc atgatcactt
     1861 gatgacagta ttcataataa tgtaagttaa atattcgatc ctcacaacta ttataagttt
     1921 taaatagaat ttggttattt ttcagaatat cgctacgata gcatttgttc catacaacat
     1981 acatcaactg ttcattcatt aactgataca tgtcttttat aaactcttcg tgagttagat
     2041 agtgacattc tgtaactaag ttttcttttt cggaaacgat tcttccctcg tccattgtac
     2101 gaaaaacaaa cgaactaaca acaagatccg cttttggact cttttctagc aaatgatcat
     2161 aatctgacaa gatcgttttt actggcgtgt catctgcatc gaagaataag agatattctc
     2221 cttttgactg tcttattcct tcgtttcttg ctaatccggg cccttgattg gtcgtagtaa
     2281 tcaattcaaa acgttcatca ttttcaatca gttcttgaat gatttcctgt gtttgatctg
     2341 ttgatccatc atttactaaa acgacttcgt actcttcata atccaattct tttagaatat
     2401 caatcgttcg accaatagtt gcttctgcat tgtatactgg tacgattatg ctgaatttcg
     2461 gattgttcat ttttacgcct cccaactagc gaaagatttt ttcattcgga aaagcaattt
     2521 ctgccaatga gatcacattt tcgtcttctt caagtccttg agaggacatt ttatcaaata
     2581 ggatcttgat ggtttgcagc aagatcttta aatcaagcat caatgaatat tttttgatat
     2641 aaagtaaatc aaaacgtaat ttactgttgt aatcagagac atattttcca tagacttgtg
     2701 catacccagt gataccagca cgaacattat gccgtaagta gtaataaggg ttttctgcat
     2761 tgaactgatc gacgaagaaa ggacgttcag gacgtggtcc tacgatcgac atatcgcctt
     2821 tcagcacatt gaagatttgt ggtaattcat caaagcggac agctcgaata aatttaccta
     2881 caggcgttac tcgtgcatca ttgctcttag ctaaaactgg tccagatttt gcttcggctg
     2941 tggcggtcat cgagcggaat ttatagattg aaaattcctt ttgatttttt gtgatccgca
     3001 cttgtttata gatgattggt cccggtgatg tcactttgac taaaattgct gtgaataaca
     3061 taaatggtga agcaataact aataaaacaa gtgaaacgat gatatcaaac aaacgtttga
     3121 atagttcata ttcaggttgg attctaaatc cagatgcttc aataatactt tcatcttcga
     3181 aattcataat atttggattg atcaatgtca agttttcaaa tgttgtattc aaaaacaatt
     3241 ttttttcatt tttagtaact aattgataga ttttgatttt ttcagtttct tcgatatgag
     3301 aagcaagata gacaatatcg atgtctttaa tcatattttt gatgtttgca taatagttat
     3361 caagaatgac tgcttcgact ttatgtttgt tgtttttctt tgaaccaaaa ttttggattg
     3421 ctggtgcgac atccttttct aacccaataa tgacgacctt tttatctcga cttacgcgta
     3481 aataaagttt atatacgaga atacgataga tacttaagaa aagcgtaccg agaacaaatg
     3541 aaagtaagat aacaagacgt ggaaaagcaa accagcggcc cgcaaaagtc actaccatga
     3601 tccctagtgt catcaatacc tgaccgatga ctgtgacaaa cacgatgtca ctgatcattc
     3661 gattataaaa aacatatgca cccaacaaaa tgttcaacgc aataaaaatc actgaaataa
     3721 taattgctga gtgttggaaa gtttctaaat ttcgtaatgg gatctctcca cgaaacttca
     3781 gtaagaaact actatatatt gctgcattgt atacaataac atccattaat ataatgaata
     3841 tccgtcgtgc agcactccat tctccatttt tattcatata tacactccta atcacaaaat
     3901 aatatctcta ttatcttacc atagtcacct ttaaaaacct agtttataag caaattgtaa
     3961 tggaatttaa aaatgtctaa taaaaagctt tatacacctc taatcaagac aaggactcta
     4021 aattacaaat ctccagatga atcgattttc actaatgttg gtaaattaaa gaagtcgaag
     4081 tatttgctgc tttttttatc cactcataaa gtgaaccttt atatttttca gagaatccat
     4141 cttcccaaag cgctgtctag tccataagaa tacctgtatt acgtgaagaa tcgaaaaaat
     4201 tgaaatcgat ttttaatgac attatgctta tagaagaaag tgaggtaata tcttatgaat
     4261 aaacaagagt tgcatgattt tattggtttt cgacaaccaa atatatgtca aattgtcgca
     4321 tataaggatg gggtagaagt ttatacagat gaatggaatg gttataaaaa agacgatact
     4381 tgtcatatca tgtcagcaac gaaaagtatt gtatctttgc tgattggcat tgctttagag
     4441 caaggattaa tcaaaagtat cgatgataaa gtattagatt attttccaga gtataaagtg
     4501 aaacgcgggg agaaaactat ttacgctatt acaatcaagc acttgctaac aatgaaagca
     4561 ccctacaaat gtaaaggcga tccatggaca aaagtctgtt caagtgaaga ttggacaaaa
     4621 acatcactag atttattggg aggaagaaaa ggtataaccg gcgaattcaa ctatcaaact
     4681 gtctgtttac acatattaac tggcttgttg tatcagacaa gtaagctaac aactgttgat
     4741 tttgccaata agtatctatt tgatcctata gggataccaa accatgtcaa ttattatgct
     4801 gaaacagctg aagaacataa gcagtttaca atggataagt ctcctaaaac caatgtctgg
     4861 ttttgtgatc cactaaatat cgggacggca ggatatggtt tgtgtttatc ggcaagtgat
     4921 ctggcaaaaa ttggtgtatt atgcctaaat aaagggatgt ataaaggaaa acaaatcgta
     4981 ccacccatgt ggataaacga gatgttcaaa cctagaacca gtgctttaga agacttacaa
     5041 aatatgagat atggctattt atggtgggta ttagattctg agaacgaaat atattcagca
     5101 attggtaata gtggaaatgt tctttatatt gacgctaaga acaatctggt tatctccatc
     5161 acttcttatt ttaaacccat gataattgat agagtggctt ttgtagagga ataccttaaa
     5221 ccatttattt taaaatagcc tatattaaaa taaaatatcg attctttgac agctagggtt
     5281 cgtgaaactt tggctatgat tttgtatcta ttggcgctct gagcatgaat aagagcgtgg
     5341 gacaaaagta agaaacactt ttgtctcatt ctttaaaaac gaataaacgg tgggagcaga
     5401 agcaacttct tcttaaataa gccgaaattc acaaaaattt gaaaagcaat tttcgtgaat
     5461 ttcttcttat ttctcggagt taaacacttc tgtcccaacc tcgacttatt tttttgagtc
     5521 ggcaccttct gccttttttt gacaaaaaac tataaaaagc cgttatgtgc gagcgaatca
     5581 caacataaca gctttttatt atagtttaat ctggtctcaa cgaaaagtcg ccgattcctt
     5641 cttttgttaa attatcattg taaaatggat catgcgcaat atatgctggc catttatctt
     5701 gcattttttt gatttcacca gcaaaacgtc tttgtttttc tggagtatct tcataacctc
     5761 ttgatttaga ttcgaaatgg tacaattctg cttgatgtgc atagacattg taacgaccca
     5821 gttcataaac tttcaagcac aagtcaacat cgttgaatgc tacttctaat gtttcatcaa
     5881 acccgttaac agcattgaag tcagctgcct tgaccatcat acaagctgca gtgactgcta
     5941 agtaattgac gtcaatgacc aatcggccga aataaccaca atgtgttcga tcatagttat
     6001 tcaatgcatg ccctgcaaca ccaccgattc ctaataatac tcctgcatgc tgcgttgtat
     6061 catctggata aaacagtttt gctcctacac agccgattcg atcaaactgt gcataagaaa
     6121 ccatcaccgt catccaatct ggctcaatca cttctgtatc gttattcaag aataaaaagt
     6181 atttgccgtt tgctttttct gctgctaaat tgttaattcg agaataattg aatggaatgt
     6241 caatcaattc tacgataaag cggtctttca gctgatgctt gtattctgca aataattctt
     6301 gcatttttgg atctgtactt ccgttatccg caataatgat ttcataattt gaataagacg
     6361 ttttttcgat gatcgaatcc acacaagttt ttagatcttc atagccattc ttcgttggaa
     6421 tgatcacact gaccaaatcc tcttgcaaga cgtcataagc aatctcataa aatccagaaa
     6481 ttctgccagg acgtacgctt cctttgatgt ttcttctaga cagggcatct gtcaatgctt
     6541 tcactccgga atcatagata taacttttgg cttcgccatt acttgcagtt gatcctggga
     6601 tcgtccgcca atgatacaag actcggtcaa tgtggtaaat atgatctgca gggatttgtt
     6661 ctgtcacacg aaggaccaaa tcataatctt gagaaccttc atagccttta cggaaaccgc
     6721 caagttcttt aacaatgctt gttcgatata cgcctaagtg agagatataa ttatttccca
     6781 tcaatgtatc aggcgaccag tctgctttga aatgcggatc aaagcgattg ccatctgcat
     6841 cgattttatc ttcgtcactg tagatcacat ccagttctgg atgtacgttt agcacttttg
     6901 ccacttcata taatgcaaat ggcggtaatt catcatcatt atctaagagt gctataaatt
     6961 ctccttctgc tatttccaat gcagaattag ttgccagact gatgtgtcca ttttcttttc
     7021 ggaaaacgac tttgattcta tcatcttttg cttgatagga ttccagacac tttcggatag
     7081 ctggatcagt agaagcatca tcactgatac atagttccca atgatcatac gtttgatcca
     7141 gtaccgaatc aatgcatttt tcgagccatt tgatttcaac gttatagaca ggcataagta
     7201 tggaaatcaa tggacggtac gcaaatcctt gtgcttcttt cctttgctct ttcaaatctg
     7261 gtttctcatg acgactgatc cactctgcat agtcgatcga tcctggcttc aattccaatt
     7321 tgacccgacg gatcgctttt tttacgccat gagtccttgc ataatttatc ccttttttca
     7381 gtaatctttt ttttctttcc catgaagatt caatgccgtc atcataaggg tatttaccgt
     7441 ttaatttcac agctatgcca ttttgatgtc tcgctgtttg gaagtctaag atggcttttc
     7501 ctctcatttt ccctgataaa cgtaccttaa aaccgctttg ggtcttaaca tcaagattat
     7561 ataaatgatt gatatccaat cttacgacat gtgttgcttc agcaatcaca ttttcttttt
     7621 ctacctttat cgtagggatt tctttggtca cttcatcaat tgcccagccc acaataagca
     7681 aatcgccagt ggcccgttca cgagtgattt gatcgataaa tacggcaatt tctttttttt
     7741 gcacgattcg tttactcctt atctttataa gttaatcatt atttacattt caatgatcca
     7801 tgattggcca ttttgagcta ctgcttccac taagaatgaa cgttcttgct cgcaaaggat
     7861 tcgtaactca aatcccgctt ctgttccttt tgcgagctgt tctgcttgat tgacttcttc
     7921 tcgtgaaaca ggaattgctt cgaaaaatgg cgattggtca gctgataact tataggacaa
     7981 gggctggcga tccgaattag cgattcccca tccgatgatt tttaatattt ttgtttctgc
     8041 atcccagatt ttctcgtcaa tacatataga aagttctttt tcttggattt ttttcgaagt
     8101 ttctttctta cgtcgtccta gtctatgttt tattgtccat tcatcacttt ccaacaatgt
     8161 gcaatagcga tttgtggttg tttttaacgt atggtctagt cttgtatttt cctcaagaag
     8221 atgatgattt tctctttcta attcgtcatt tttctcagaa agctgctttg tgacttcagc
     8281 catgggacga atcgtttcca acaattcttt cattgttggt gtcaattccc cgataaagcg
     8341 atagtgacaa tggatagtca cttctttccc agaaatatcc gttagaacga actctggtaa
     8401 ttctttccca tcaaacaaat aacaatcatt tactgttttg actacagcat tcgaatcgat
     8461 aaatgttagt ttttctccgg cagcttctgc gctgaattcg ataaagctag gttgttgcgc
     8521 gaagcgaaac accattcttt ctgttgtttc tgatacgggg aaacttagtg tctcattttc
     8581 ccctgtgaaa ttattgaatg gtatttgttg gattgtttca tcttgtttcg tttgctgggt
     8641 tacttctaac acttttacat aattagaatt ttgtggctct gcaatggaac tttgtgctac
     8701 tggatgtttc atcaatgaga aaaagtattg gtagacttca ccaaatggtc gcattttgaa
     8761 atcatagcgt acagcttccg gcaattcttc ataagttgat tttaattctg tatcacctac
     8821 agctaagtac aaataatctt caatattaat gaataatccc gctttttcaa aaactttttg
     8881 gaagccatca tgagtataaa aagaattatg ggtttcatct aggagaccat aagaagccca
     8941 cggcaattgg ttattgaaca aatcgatcat gacagaatta tgcgccaagt tagggaatgt
     9001 aatcaggatc tctccttctt cattcaaaaa ttctctgact ttcttcaatg tttttccagg
     9061 atctaccaaa tgttctaaaa catccgcaaa caagacgtaa tcaaatgtct gtccagcata
     9121 atagtttgcc cactcaaaac tttcgatatc accataaaat ccatcttgag caaattcgct
     9181 gacaaaatcg aacaattcct tatctaactc aacaatgctc acttcacatt gttttgctcc
     9241 gattaaatgt ttcgttaatc ggccgtttcc gggaccaaac tctaaaactt tacttcctgg
     9301 tttgatctgt gcggcaattt ttcccacact gctttgttca tctaggtcca tttcaaaatc
     9361 atatttcatt gctatgagtc ccttcttccc atttatggtc taagaccacg atgccttctc
     9421 cgatataaga accgctgaca aacaagttca tgtactttgt cttatatacc aaaggaaccg
     9481 ttgcattttc ttcaaagacc gcaacgtcaa aataatattc accagctaac aaacctaact
     9541 tttcatactc cagataaaag atattctctc cttttttcca aggaatcttg ataccatcta
     9601 gtagagtatt aagtccgcat acataaaaat tgtcgactgt tcgtagcgca attcctaaaa
     9661 ccggattttt gattgtatca tcttttactt cataagttac tttaacgtat agtttttctc
     9721 cttgttccag catatccacc ggatttttgt ctttatccaa caattccgct ttcgtcacgt
     9781 cgacgatatc tggagcggcg aaccgttcta cttggttttt attccgatcc actgttttaa
     9841 ctgatttttt ctttaaaaag ttttcataat tcgtcgttac ttccatcgta tcgccatatt
     9901 cgacgacttc tccgtttttc aaccaatagg aacgatcaca aaaacgacga atcgaactga
     9961 tatcatgaga aacgaataaa atcgtctttc ccgagttctt gatttctgta aatttttcca
    10021 tacattttag ttgaaattca agatcgccta ctgccaatgc ttcatccaca atcaaaatat
    10081 cgggattcac attgattgct acagcaaaag ctagacgaac gaacatcccg cttgaataag
    10141 tttttacagg ttgataaata tggtctccga tgtcagcaaa ttcaatgata tcatcgacca
    10201 tttcatcgac ttcttctctt gaaaatccta gaaccattcc atttaaatag atattttcat
    10261 agccagaata ctcaggattg aatcctgatc ctagttctag caaagctgag atttttcctt
    10321 cgatttccat cgtgccggat gttggtgtca gtactcccgt aatgattttc aacattgtag
    10381 attttcccga tccgttttct cccacgaaac cgatcatctc accttttttt atttctacat
    10441 tcacgttttt caacgcataa aatacatcat gataggattt cttaaaagga ttgagggctt
    10501 cacgaaatcg atccgaaggt tttgcgtaca tattataaga ttttgtgata tcttttagtc
    10561 taattgcata ttctgtcata gctattctcc ttacagcaca tctgaaaaat gtggtttcaa
    10621 tctacggaac actgcagagc cgattacgaa cattactaac gtgaaaaccc agaaatacaa
    10681 agtatatgtt ccatgctccc agaaccaagc ttcacttaaa aatgactcac gataaccttg
    10741 aacaacatag tacagcggat ttaatttcaa tatgcttaca agacctgaat caaactgatt
    10801 agggctccat aatatcggta atgtccacat gatcaactgc agcacgatcg taattaattg
    10861 catgatatca ggtaaaaatg gttggatcga tgccgtcagc caagtcaatc ctgtcaagaa
    10921 agcacacatg caaaataaat aataaatcag ctgtaaagac attgctgaag gatatatacc
    10981 atttataagc gcgacagcta tgccaatcag aatgaagaat aagtgggtgt acagattgga
    11041 taggattttc gtcgtgggca agatacgaat attgaatacg actttcttta ctaaataagt
    11101 atattcccga aaaacaaccg tagttgatgc aaatgcatct gagaagaaga accagggaat
    11161 catcccggta actaaatata cgataaatgg gtattttgca tcgatcattt gtgctttcag
    11221 tccaacacca aataccagcc aataaatcaa tacagtaaat agcggattga taaacgccca
    11281 taagattcct aaaaacgatc ctgcatatct tgatttgaag tcattgatcg aaaactgtag
    11341 caataatttt ctgttctgat aaatgtcctt cataaataaa tacaaatctt taaacatttg
    11401 tgttcctcca agcgattttc agtgaattac agtcaagtgc cattttacca tatttttggt
    11461 accgagctgc gttcttattt ctttcttaat tttctatgat acaagatttt ctctaacgtt
    11521 tctattttac agaagatgct agacaattgc aataaaccaa aaaaggctgc gacggttgtc
    11581 acagcctctc tcgtattttg tgcctatcaa tgatagcgtt ggatcctgga agaatgagcg
    11641 ctatctggtt gttcctcata agatgtatca tcataagtca tatcgtcata tgcctcttcc
    11701 ataacataga attcctccgc aacttctggt tcatcatatc gatgaacggc catagccaac
    11761 aggatataaa taatcggaac ccatgggtat gaagtcaatg tatcctccat aaatgactgc
    11821 atcatcaccg caaaaaaggc ccagataaaa gtcatctgcc cgttggataa atctgctttc
    11881 aacgttaagc gaccaataaa aaggataagc agcaaagcag caatcccaaa tcctatcaat
    11941 ccatagatat tcaaaataga attataacta ttgtggtttc ctaaaaattg gtttcctcgt
    12001 tggaagataa atgttttcat tcctagtaaa atttgttcag atttttctcc tagcgtctga
    12061 tagaatttat gccaaatatc ttctcgaccg gtaaacaaat tgactttttc agagacagcc
    12121 gccagataag aaatcggcaa agctaaaaga atcgtaaccg tgtaagcaat aaatggcgca
    12181 cgaatgacac gcatcaacga tttaggtaaa agatccaata tcgcaaaaac aatcaacgcg
    12241 actaatgcac ctctagattg gcagacccaa atcccgagaa aagctgcaac aactgctact
    12301 gcaccgatga ttcgtacata ccatcgttca aaagaactgg caaatccact gatcaataaa
    12361 gcaagagtca tcaaagaact tccgattata ttcgtattga tccaaatatc agaaagcgaa
    12421 ttgcctggag cccagatgct ttgtccttct ggtacttgga tctctgtaaa tacacgatat
    12481 aacgtgattc cgaggaagac cgaaaagaca atcagataaa taccgcgatc ccattttgtc
    12541 cactcgatcc atttaattac taaacaaaat aaaataatca aaggaaacag taagtctcca
    12601 aataaagact catttcttaa ttcccgaaag taagaaaaga aaatgaaaag ggcaagcgca
    12661 acacctagat aaagaaaatc gacgcctttt gctttataaa catttaaaat gagcaaaata
    12721 gccacactaa aagcgataag taatgaacta ttgttatata taaacaaagt tgctggaaaa
    12781 cctaaccctg ctgtccaaac aaaaaagact agcgtcagta acaaaagcaa gaaaccgaca
    12841 ctcattttgt tgacaaattt ttgtacgatt gcttgaatta tctccactct cctttctact
    12901 aacatttcag gattattctc agctagccta ttactgtacg actagctgat tttcctgagt
    12961 ttgtgtttgt ggtacatctg gataatccgg atattctact ttaaaaatca tataaccgtc
    13021 tttatccggt ccatatagct gcgtgaactg cgcttcatat aattggttgt aatcagttaa
    13081 tggtcgttta ctgatcacat agtcgatccc taatttttct gcgtcttcaa aattcaacgt
    13141 gaccgaaaac atatctggac gatctaaaat gaactttgta tcttccttag cgataaaagc
    13201 tcttgtatga gcataccgat tataatactt ctcgtattct tctttcggat cgattttctt
    13261 ccatgcagct atatctggat aaaagcgtac tgtattgaag gatttcactc ccaatgttgg
    13321 ggtgaagtta tataattcat cttcagacaa ccaaacttta ccaggatctt gttgatcgat
    13381 tttttggatt tcttttgcca gtgttttttc aaaaatcgct cctgtacctt ctacgactgg
    13441 attgacgaaa aagccagata cgataactaa ggcggcaaaa gcgaggtaaa acaagcgtct
    13501 ccagccaaat aatgcaaaag ctaaaatcac tgttcccaac acagcaactg cgatggtttc
    13561 aaaatccgaa aaataattat ccattttcga atgcgtaatc gcatagaaat aagaccatgc
    13621 atttactgcc aacaaaatga atttatacca aataggaata acatcttctc tccaaatata
    13681 agcgataaac cagatcgata ataaacaagc cgcaaaacta aaagtaagga tggctcttaa
    13741 tggaggaaca taagacagca gagtgatttc tgctagccat tttggcaacc caacggtaat
    13801 ccaaaagata gaaaacagac aaaaaatcat caaaatacgt cccagtaact tttgttcgct
    13861 atcttttttg ccaaataaca caaatggact tgctaggaat acagttggaa aaaagtgata
    13921 gaacagggcc acttcagagt tattcgaata gttgatatct tcaaaaggaa ttttccaatt
    13981 tgtaaggaaa gaaaaaaatt cgccaatcgt ccactttcct ccagtactta cccgttttcc
    14041 tggatataag gtattcaaag aagctaacaa tgcgtctttt gacgttagcc agaaatgaac
    14101 catgactccg ccaataaata aaactgcccc tccaatcaac actacatcca acagatccca
    14161 tgaaactttc ttgctatagt aaataagcag gccaacaaaa taaaaaatca gcaagtacgc
    14221 cagcatcact tgatgggctg ggtaaatcac taaaataaac ccaataccga aaataaccac
    14281 caaaagcatc atcaacgttc gtagccattt tttatcatga ttgtagaaat aatgataaaa
    14341 agcagtcatc agtcccaaag taaagaaaat caaatcgccc acatgttgca tgaaccacca
    14401 ctggactgct ggagaaaatg tcagcgcaaa tgcaccgatt agcgatagag ctttttttct
    14461 cttcgttaat atcatcagta cttcgaatcc aagcaagacc atgacgacta atttcatcgc
    14521 ccaataaaag gaaagtcctc gatctcttcc gaggaaaaag aatccccagt tgaatggttt
    14581 tccgataacc gtaatatcct ttacaggaga attatacgca atgatcatgt tttgtccgtt
    14641 taatgaataa tcctcgttca ccaacggaaa gtcctcttca gcttgtgaga ggtaaaaagg
    14701 tgtctgcacc agccattcgt ctgaccgcac ttcccgattc tgtccaaaaa tcacatcagt
    14761 ttttttccca tcgtctcttt gcgacacata tttatcccaa acaccaagtg aacttccgtt
    14821 taaattaaaa gtaacgatga ccacgatgac taaaacgcca ataagatagc ggagttttag
    14881 aaacaggtca agcaccaaat tcgaatctgc ctggttcgct tttggctttt cttttcgtcg
    14941 tgcacttctt gtttgttttt ccatatcatg ctccaaattc tatttgataa aaggaaatta
    15001 cttattcatt tttttggcag cttccagcat ttgcgcaagt gcttcttgcc aagttggtat
    15061 gacaaatcct aactcttctg tctttttcag gctcatgact gaatactgcg gacgtgttgc
    15121 tttttgcgga aattcttctg acgtaacagg tgcaacttct acatctgtat ctttcaatat
    15181 ttcttttgcg aattcatacc aagaacagct attttcgttc gacaaatgat aaataccaaa
    15241 tggtgctttt tcttcgatga catataccat aaattctgcc aaagtacgtg tccaagttgg
    15301 tcgcccaaac tggtcattta ctacagtcag tttgtcgcgg gtttccgcta ggcgttgcat
    15361 cgtgaagacg aaattgtgtc catagatccc gaaaacccaa gaagttcgta tgatataata
    15421 gtcttctaaa atctcttcta ctgctttttc gcctaataat ttcgtacgac catattcatt
    15481 tagtggattc gtttggtcgt ctacttcgta ttctacattt ttgttttttc catcgaaaac
    15541 atagtctgta ctgatataga cgaattgagc attcacagct tttgctgcaa gtgctacatt
    15601 tcttgtacca tcaacattga tcttctcatc taattctttt gcttcttctt ctgctttatc
    15661 tactgctgtg taagctgcac aatggaaaat aacatcaggt tttaattctg tgatgtaagc
    15721 cattgttttc tcggcatctg tgatatccaa gtcttttgca tctgttgaaa catattgcag
    15781 atttttttca tctaataaat ggcgcaattc tgtacctagt tgcccatttc ctccagttaa
    15841 taaaaacata tgattatccg ctccttgatt tttattttaa aaaagagaca ggcgagcgcc
    15901 tgcctcacat gtgtttttcc attaatacag tgacattttc tctgtattat tgtccatttt
    15961 tggcatatgc tgcttcaacg gcctcttttt cttttttcca ccagtcttca tgttcttcgt
    16021 accatttgat cgtgtcagcc aacccatcac ggaagttcgt aaactcaggc ttccatccta
    16081 actcttcccg taattttgtc gaatcgattg cataacgcaa gtcgtgtcct gcacggtcgt
    16141 ttacatggtc gtacgcatct tttggttgac ccatcatttc taagatcaat tccatcacgg
    16201 tcttattatc ttcttcaccg tctgcaccga tcagataggt ttcgccaatt ctgcctttcg
    16261 tcaaaatcag ccacactgct gaagaatgat cattcgtatg gatccagtca cgaacattct
    16321 ttccttcgcc atataatttt ggacgaatac cgcttaagat attcgttact tggcgaggaa
    16381 taaatttttc aatatgctga taaggaccat agttgtttga acagtttgaa atggttgcac
    16441 gtaagccaaa agaacgcacc catgctttta ctaacagatc tgaaccagct tttgtagaag
    16501 aatatggact tgaaggatta tatggtgttt ccgcagtgaa tttttctcct tcgccttccc
    16561 catgtcctgg caggtcttca cgtaatggaa gatcgccgta aacctcgtca gtagatacgt
    16621 gatgatagcg tacatcatac ttgcggcaag cttctaaaag tgtatacgta ccaatgatat
    16681 tcgtttgtac aaatgggaaa ggatctttta atgaattatc gttatgggat tctgccgcgt
    16741 aatgaacaac tgcgtccgct tcttttacta gacggtccac taattctgca tctgcaacat
    16801 ctcctacgac caattctacg cgatcgctag gaagtccagc caagttctct ttgtttcctg
    16861 cataagtcag tttatccaaa acagtgacat gcacttctgg atgattgttt accacgtaat
    16921 gaacgaaatt agagccgatg aatccggcac ctccggtaac aatgatgttt ttcataagaa
    16981 aatttctcct taattcttag atttctccgt aaacaaatgg attttctgct tcaaattctt
    17041 ttaatgtcgg atgacggtga tctttttccg acataatcgc tcttgatgga tcaattggcc
    17101 aatcaatcgc taaatctgga tcatcaaatg cgatgccccc atctgcttct gcattatagt
    17161 aattatcaca cttataaagg aagttcacat ttggtgtcaa agtaacgaat ccatgggcat
    17221 atcctttagg tacaagcaac tgtcgatgat tatgttcaga taagataaat ccgtcccatt
    17281 gaccataagt cgggctacct ttgcggatat ccactactac atctaaaact gctccagtga
    17341 ctacccgaat caattttgtc tgtgcagctt ctcctttttg gaaatgaagc ccccgaagga
    17401 caccagcttc agctgatagt gaatggttgt cttgaacaaa gtcatatttc aatccatgtt
    17461 ctgcaaattt tgcttttgaa taactttctg tgaagaaacc gcgatgatcg ccaaagacgt
    17521 ccatttctac gattttgaca tctgttaatt ttgtatctgt tactttcatc tgtaaggact
    17581 ccttattcag cggccaaatt caataaatat tgaccgtatt gatttttttt caatggctgt
    17641 gcaagggcca gcaattgttc cttatcgatg tagcccatac ggtaagcaat ttcttccaaa
    17701 caagccactt ttaagttctg acgtttttca attgtttcga taaaggtaga tgcttcaagc
    17761 aatgattcat gtgttcctgt atcaagccaa gcaaatccgc gtcccataag ttctacggat
    17821 agttttcctt tatccaaata tgctttattg acatccgtaa tctctagttc tccacgttct
    17881 gaaggtttga tacctttagc gatagaaatt acctcattgt cataaaaata aaggccagtt
    17941 acagcgtaat ttgattttgg ctgagcaggc ttttcttcaa tcgacaaagc ttgcatattc
    18001 tcatcaaatt ctactacacc aaagcgttct ggatcattga catgatagcc aaatactgtc
    18061 gcgcctgtat cttttgaagc tgctcgttgc aacattttag acagaccgcc accatagtaa
    18121 atattgtccc ctaataccaa gcagacacta tcatctccaa taaattcttc tccaatgata
    18181 aacgcttgtg ccaatccatc tggactttct tgtactgcat attcgatctg cagtcctaaa
    18241 tcatttccgt ttccaaataa ttccttgaaa cgtggtgtat cttgtggcgt tgaaataatc
    18301 agaatttctt tgattcccgc caacatcaga gtagacattg gataataaat cattggtttg
    18361 tcataaatcg gcatcaattg tttcgatgtt gcctttgtta aagggtacag acgtgttccg
    18421 ctgcccccag caagaatgat tcccttcatg gatatctgct ctcctttttg atttcaaatt
    18481 tcactttgtt attatctcat caattgttat atttgtcatg gaaatattaa ggatttaaac
    18541 atcttttatc aacgtttaaa ccattttcgt ttgatcaacg ccgtaatcag atgaacacgc
    18601 caagaaattt tttgccgtaa tttagaatgt gtttcaatct cactcgcgtt taaatcatgt
    18661 cttcggtaca gagttaacgg ttctcttagg aacccagtct tctttcctcc cagcaaacca
    18721 atccacatat catgcatcgg cacttctgac gggatcggca aaatatcggg ttttaaagaa
    18781 gaccggaaag ccataccagc accaatatag tgacttctta tcacattatt aagccatcct
    18841 tctttggaat ttcgaaacga aaaatacgaa tcatggatct cttgcagctc gctgtccaca
    18901 atgattaaat cagaaactac tactagctga tccggattct gctcaaaata gtcagtgatc
    18961 tttttcactt ttgtctccaa ccaaacatca tcttggtctg ccaaaaagat tagatctcct
    19021 ttcgtctgct ggatcgcata accaaaatta gcgatcaatc cttgacgagg ccccgaaaaa
    19081 agctggattc gcttatcttt ttctgcgtat tcagttaata tatcccatgt atcatctact
    19141 gacccatcat cagaaatgac tacttcatct tctgctgaaa gctgagataa aatactatct
    19201 aactgctctc ttaaatatct gcctccatta aaggtagcta tacaaaccga acgcacgaat
    19261 tcacaccttc caattcttaa catatgagcg caaagtcatc cgccagatat gccggttttt
    19321 gacggtcaag aactgtttag ctgttcttaa caataattgt cgtcggtgac tggataaatg
    19381 cctttgatca tattcatgat aaaatcgatt ttctgcttgt aaaatagaac ggtatcgatt
    19441 acttgaaact ttttcatagt ctaaaaccga aagatcatgt tccattcgta caggaaggat
    19501 ctccacagtt ttttccaact gatccacttt ccaaaacagc caatgatcca aaaaatctaa
    19561 agaaaaagca ggattaaacc cgccaatctt ttttagaaat ttgacagacc aggcaacacc
    19621 tgaattgatt gccatgattc gttcagctgt tgggccttca acttctactg cttgagctgt
    19681 acgattgata taacgatctg aatatagggg agaaacttgt cggctcccag aaaaaatcat
    19741 cggaactgca gcaacacatt catctgtaaa ggttacttta cgcaatagtt caaaataatc
    19801 gtcgactagc ctcgtatcct gatccagagt gacgagacaa cgtgttcctt catctgcttg
    19861 agacaacgca aaattataag ccaccgctaa accaggattt tgcggatcat gatgataaac
    19921 gatattttct ttttcaaaca gctcattttg ctgcctttga ggactattat cgtaaataat
    19981 aagctgaatt tgcttttcgc taagtgcttt gcttaatggt ccataagctg gagattgact
    20041 gaatagttgc tgataaagca caacgactac taatatttct ggtttcatca gaaaaaacgc
    20101 cacccccact tattgaaaaa tttcatcata gaagtcaaaa acacgcgaaa cagttttgca
    20161 tttcgatgcg cacccttccc gtaaaaatgg ataactgcat aaaagggtgt gtacaataac
    20221 ttgtaacctg ctttttcaaa actcaaacac aagtcgttgt cttcaaagta catgaagaaa
    20281 cgctcatcaa acccctttat ctctttaaat ttatcaatat cagctaccat aaaactacct
    20341 gagatcatcc gtacataact attagcatca gtaggcaatc ctcggcattc aaacacagcc
    20401 agtcgtttgt caaacttttt tttgacccat ttgaaaggca caaagcgcaa aacgtaatca
    20461 aaaacagcca gctgctcacg gatgaggtgc tgagttgttc catcttcatt aagaatctta
    20521 ggtgctaaca tcgcacattc cggatgttct ttcaataaca agaccaatgc tgtaactgtc
    20581 tcttcatcca ctaaaatatc tgggttacaa ataatgccat agcgagaatc gctagctatc
    20641 agattgtgat tatgtccata accaaatccg gaattttcat catgaaaata gatatcgatg
    20701 tagggatatt tcctatactc tgttagtctt tttttgtact cgggaagtga attgttgtca
    20761 aaaattcgga tttttactgc tgtttcatct tttacagttg aaataaaatt atctagtgtc
    20821 cgaaaaattt tatcactgtt tgccgtcaca atactaataa ggatcggctg gtttaatttg
    20881 ttattcatct atatgacggt tccatttctt caaatatttc tgttttactt gcgtacggta
    20941 ctctttattc cctaaaaatc gcaatgtttt taaaataaac tgatgtttct cattgatcag
    21001 accaatcaat tcaaataata aaattgcagc aaacaacatg gcaataatca atatgatcgt
    21061 tccgattctt ccagtataat tcatgaccat agctgtgaaa gaaaacatca aagccatcgc
    21121 ataaatcgtc aggacagcac ctttatgagt aaagcctaaa ctgattagtc ggtgatgaag
    21181 atgtttttta tctgctgtag aaaaagaaac acggtttgct tttctcctaa tgatagcaaa
    21241 aaacgtgtct gttaccggca ctcccatgac gatcagcaaa gaaatcgatg aaacaaaagt
    21301 cacgttcttc agcccttgta gtgaaaggac ggccatcata aaccctagga atagagctcc
    21361 tgtatcgcct aaataaatct ttgccggata aaaattgtaa ggaaaaaaac cgataatact
    21421 tgctacaaga cagaaaataa caactggaat atacactgta gaagcatgga ggaaaaaata
    21481 tccaatgatt cctatcgtaa ttaaaccgat caatgaaatc cctgccgcca gcccatccaa
    21541 accgtcaatc agattgaccg cattcgtgat accgaagatc caaaaaatcg ttacgggaaa
    21601 actaagccag cctagttgga tatatccaat aaaaggaagc gtgaaggaat caatacgtat
    21661 atctgctaca aaataaataa taagagaagc aatcaagatc cctatacttt tttgtttagg
    21721 tgtcagctca tatttatcat cgagcactcc tgtgactatg attactaaag aagcaatcaa
    21781 gatcggtaca atatacgatt gagggataat tttacggaat aagaataaag tcgatataac
    21841 aaatgaaata aagataggca gtccgcccgc tgaaggcatt ggaactttat ttattcttct
    21901 ttcatttggt ttgtcgacca tacctgtttg tactgaaatg attttgaata caggtgttaa
    21961 tatcagtgat aataaaattg tcagaaaaaa tcttataacc atactaaaag aaacaagcat
    22021 atgggcacct actttaatta agagtcataa ttattataaa ttatatatgt ctttttgact
    22081 atctttttat atcttctcaa atgtccgcaa agccttattt tatcggctct acgggcattt
    22141 tgcttttgtg gtaaacctcg catatctagg tctatcttct tatattttcc ctgtcaatcg
    22201 tggttaaaat cgtggtaaat acttctatgc gattagacgc tgaacctctg attttgcggt
    22261 atctatggac gcatgagcgt accagttcat tgtgatactg atgtttgaat gccccatgat
    22321 atactgtaaa tctttcgggt tcatgttctt gcttgccagc cttgtgcaga atgtatggcg
    22381 tagcgtatgc ggtgtaatat gtggcaaggg attgtccttg tggtgcttgt tgtatttctt
    22441 taccatacgg ataaataagg cgttataatc aatggcaact tttggattac ctttcggatt
    22501 gataaacaca aaaccgctgt gtccgtctac ctctatcgac tttatctttg gtcggctgtt
    22561 gataacccgt tgaaatgcct gtatcgtttc tttgcttaat ggcacttgcc ttatcccgct
    22621 tttcgtctta ggcgtttcaa tataatagcc ctgttcctta ctctttagta actggtggtc
    22681 gataatcaca acttcattct tgaaatcaat gtcggctact gtcagtccgc acagttccga
    22741 gatacgaagt cctgtcttta acagtatcag cacatcatca taatgcttgt gatacacgtt
    22801 gtccgtctta ataaatgaca ataaggcttg ctcctgttcc tctgtcaatg cgactttctc
    22861 tttggtatca ttttctatga cttcacttag cttgaaatca aaagggtttt tcctcacata
    22921 atcatcttgt atggctatgt agaatgacgc ttttaacgag cgtttatggt tgttaatggt
    22981 attgtaggaa aagcctttgt ctttcatgcg taacgcccat tctttagcgt cagagggttt
    23041 tatcgtatca atgctcctag cacctaactt gtcctctttt aataaccgca tgagttgttc
    23101 ccgttgtttc tgtgtgcttt tcttcacatt tgtcctctgt gcgttctgtt tggcatagag
    23161 ttggcaaagc gtcattttct tgcctgtgct gtcgataccg tcctcaatat cccgtcttat
    23221 ctgctgttcc agttctcgaa gtgagggttt ttcccgtttt ccctttggtg tcgggtctgt
    23281 gggtgtcaat ctccaagcat acacatattt tgtgtttccc aatgcgtcta catatttata
    23341 taagtatttt ccgtctgttc gttggctctc tccagtatgc aggatacgac ctttgttatc
    23401 ccgtcttttt tctttcatgg tgtctgctcc tttccttgtt ggaaagagcc ttgatatgac
    23461 ttgtgttcat cataacacat acaaggctca tttgcattag attgcgtcca atttatcaat
    23521 aaccttttca aactgtcggc gtttaatctg tatgcggttg ccattcataa tgagccagcc
    23581 agcgtccttg ttttcctctg ccaatcgtct taacttgttt tctccaatgc ggaaatactt
    23641 tgacgcttct tcaatggtaa gggtgtattt ttcccatacg ggaatatcct gtgtattact
    23701 cataaaacac cccctcactt tcgattacgt ctgatatgtc cgtacacatc tgttccacaa
    23761 gcatttcctg tttttcagaa aggtgggtac attcatcaat cattttcatg tagtttgaac
    23821 ctgtcagcct gtcgatttcc tctatcatca cgattgacgg catgacctgt ctttccagcc
    23881 attgcagagt gcgttccata ttgacaggta cagggttcat ttcaaaaggt aaactgtcat
    23941 gggaaatgaa tatctcccac aatggatagt caggaaattc cacaaaatcg gtaatgagat
    24001 aaaacaccag cccatgaggg ttatacgtca ataacagttc ttcaactgcc tgtaccgcct
    24061 ttttgtcacg cagacgaatt tcaaaacggt tgataatatc ggtatgtttc tttttcgttg
    24121 cctgttcctt ttgtttttca tacaggcaga aatattttgt gcttgccttt gagccgatat
    24181 aaagggtact tgctaaatta cggttttttc cgcttaattt tccaccttgt acattttcat
    24241 agtttttaca gcgacattcc gcaccaccat ttctgtattt ttcagatagc acaggaatat
    24301 ccagcagacc gcacatatca ttgattgcca agtcaaaacg tcggataaca ccgccacagt
    24361 caagacagcg gtttaagaat gaataccagt ccctgttctg tgcctgtaag acatattcca
    24421 tgttgcgtga gcctgcccct tttaattcta aaaatacgcc catgtgttca tcatcagaag
    24481 ccatgacctt gatttccccg taagcataat gctccttata gccataatac ccatagtcat
    24541 aatggataaa ataatctgct ttcatgccaa gcacctttcg gataacttcc aatgcgtcag
    24601 tcgttggaaa acggacagac agataatcaa cgagcagata aaaaggctta tcgcagatat
    24661 agtaattcag acaacgcagg ataatgttct gtgtttcctt gctggcgtta gcttttccgt
    24721 tctcaaagcg gttgatacta tatcttgata catgggtaag acttgcaagt tctccttgtg
    24781 aaagaccgct ggcaagacgt ttgttcttca tttcaagtgc aaattctaaa ttgttcattg
    24841 tattcctcca atcccacaat agccttgaaa tacctgtaaa ccacctttca aaaagtgtga
    24901 tatgtactat cttcttaaaa cctctcatta ctactcacag gtttgggggt gtatggtttt
    24961 tgacttgtct tttgtgaaat agtgcccccc tgttagataa cgggggcaca gacaaggctc
    25021 gcaggctcgc cagccgacaa agcatttctt ccccttgaca caaggtgtca tggcggtaag
    25081 cctgagaggc ttccgccacc gtgtcagaaa tactttgtca gcaagttctg ttttgccttt
    25141 tctaacaagt tccatttcaa ccgatattca tatctacgga tttaatcaga taggtatttc
    25201 tttttgaatg tttttatatt tcattctatt gtgcttattc gattacaaga acatagtaat
    25261 tcttatatgc ttatttgtca atatcttttt ttgcctttac aaatcatatt aagcatgata
    25321 gacttataat agcgaagata taacgagata tggagggtta tgcaatgaaa ataagccatt
    25381 taggaaataa tatacagacc ataagaaaat ttaggggaat gaaacaacag gaacttgcgg
    25441 acaaaatcgg tatcaatatg cagagccttt ccaagattga aagagggttg aactatcctg
    25501 cctatgaaac gctggaaaag ataatggaag tgctggacgt tacgcccaat gaattgttgt
    25561 cgggagaatg gaagtatgtc aatcaatctg aaaaggaagt ctgtcagttt ttaagagccg
    25621 aagaacgtct aaatgcagaa ctgaaacatg gacactatga taacttcttt gacagcgagg
    25681 aagaatggct ggaatatgag ttagaaaagt tacgggaata tattacagac tatatcaacg
    25741 ggaaaagcat tgaagcgtcc gacctttacc caatcaaaga atttatccag catttgaagt
    25801 ttcaaaaatt attagaccgc tatgatgatt tatacagtat ggatatgttt ggggaaagca
    25861 tagagggaca taagtataag acaccttatc aagtcgtgaa aatgataaac ccgaactcaa
    25921 aagaagatat ggaactgtta cgagaagtat taaggaataa caattttgat gatgaggacg
    25981 agtaactttt ttcctcttgc gagaagatac agcaaacaaa aagcccgatc aagagtgcaa
    26041 agcaccacca atggtggcaa agctcttgac agggcttttt cttttctgtt gtgggcaatc
    26101 aagaggaaga aagggataaa atctgatttg tggtaaatca ggcaaaggca acgcaggtat
    26161 cacagtatga attgacaccg cataaggaaa gcaactggaa gattgtagaa taaatgaaaa
    26221 aggcttgcat tctgcatttt tataaaattg caagtcttta tattgacaag cataaaatac
    26281 taagatatac tttaatcaga ccaacggttt gtattggagg taagttatgg cacgaaataa
    26341 atatccagaa gaaacagtaa aactcatttt agatgtttct acaaaaatgt tttgtgaaaa
    26401 aggatatgat aacacatcat tgcaagatat tatcaatgaa acaaaacttt ctaaaggtgc
    26461 tatttatcat catttcgatt caaaggaaga tattttaaaa gcaatttttc aaagaattgg
    26521 aaatgaaaat gcagtaatat ttgctaaaat tagagataac gatagtttaa acggtttaga
    26581 aaagttaaga gaaatattta agacagcggt attcaattct aaccaaagcg ttttattgac
    26641 agtttctcct caactattaa acaatcctcg ttttcttgct atgcaaatag aacaaatgta
    26701 tgaaattgta gtacctgaat ttgttgaacc cgttttagaa caaggtatca aggataagtc
    26761 tttggagatt gaaaattcac atgaaattgc agaagttatc atggtattaa gtaatgtatg
    26821 gctcaaccca ttagttgaaa tgacggataa agagggtatg gaaaaacgtt gcaatacatt
    26881 caatgccctt ttaaatggaa taggcataga cacattatta gacgaagaaa caatttctgc
    26941 atttatacag ttttgtaaat aactgtattt gttatcccta tacgatagac tgctctataa
    27001 aagagcagtt tatttaaacc attatataaa ccgacggtct gtttgattaa ataaaaagga
    27061 gagattttat gcaagcagaa aaaaaacttt ttaatcgtga ttttatactt gttgttatag
    27121 gacagataat ttctttattt ggaaacgcta tcctacgatt tgcattaccg tactatttgt
    27181 taaaagaaac tggttcatca gctttgtttg gtattgctac agcctgctct tttttaccta
    27241 tggttatttt gtcgctggta ggtggtatta ttgctgacag gacaaataaa agaaacatta
    27301 tggtcatact agattttgta acagcaatca taattttagt attttatttg tcattgggaa
    27361 aaattcctat catacctttg tttattgttg ctttgatgtt actttatggt atttctggtg
    27421 catatcaacc agcggtacaa gcaagtattc ctctattggt atcatcagat aaacttaccg
    27481 tcggtaatgc tataataaat caagtaaata ctttagcaaa ctttatcgga ccagctataa
    27541 gcggagtagt ttttcaattt tatggtatca atcccatttt aattgttagt attatatgtt
    27601 ttactttttc agccattatg gaaattttta tccatattcc acaccaaaaa agacctcgtg
    27661 aagtaggtgt aattgcagtt gctaaaaacg atttaaaaga tagttatacc tttttgaaaa
    27721 aggagaaacc catttttata tccgtaatct ttttggtttg cattttcaat ttggttttaa
    27781 gtgctgtttt aattgttggt ctgcctatct taatttctga agttttaaaa atgccagata
    27841 cactatatgg tttttcacaa tcagcattat cattaggtgg tttatgtggt ggtattttaa
    27901 cagctcttgt tgcttcaaag ttaaaactaa aaaaccttta taccttgtta cttatttgtt
    27961 ctgtttctgt tttacttatg ggaatttcgc tatttttaga attgcctgca atagtttcct
    28021 actgggtagt tacagtgatg tgctttgtgg ctatgggtgt atctactatg tttgttgttc
    28081 aaatttacac tcttgttcag acacaaacac caccgcaatt agtaggaaag ataatggcta
    28141 cgctggtagc agttgctatg tgcggtcaac ctattggaca aatggtttac ggtattttat
    28201 tcgatgtatt tatacaagaa acatgggttg ttatgtttgt agcttctatt gcttcaatca
    28261 tgatttcaat atattcaaaa agaatttttc aaaagttaga gatataatac aaggggttaa
    28321 atagtatgtt aggctaaaaa caatatataa aagaaaattt gaaatctgct aaaaggatag
    28381 tgttataaaa gaaagagtaa tattgcagta tttaaggctc gcacaaatat atgttttgtg
    28441 tgggcttttt tcttaccatt tttctgtatt tctaaagggt ataaacggta ttccgtttat
    28501 atatttcagc attagcagaa agggggtgag attatgagga catcttcttt cgagcatatc
    28561 gtcagattac agttcaattc tttaatgctg attgtcatta agaacaaagt gaaaagtcga
    28621 aacagacagc ttgcaagaca ttctaaacgt gaagtattgt tttgtgaaat agccgaaacg
    28681 aaacaagttg aacgtggaac gattgatacc tgtttttatg attgtgtatc atttaaagtc
    28741 ttgcacttta caatttatgt atctgatgaa acacttggta cagttttaca tagactgtca
    28801 gaaaaacagc gtagtgcgat tttattacgt tattttcaag gaatgaacga cagagaaatt
    28861 tcagaactgt accatgtatc acgttctgct atttgcagta gaagaactag aggactaagg
    28921 aagttaaaaa tgcttttgag cgaaaggaag taaagaaatg agtaaagatt atggctatcc
    28981 ctcttttgaa ttgatttgca aagcgtcaag cggagatgaa atggcagtta aagagatttt
    29041 gaagttttat gacggttata tttctaaatt atgcttgcgt cctttttacc actctgaaag
    29101 tggtaaaatc attatgcagg ttgatgaaga actaaaagga gaaattcata cagacatgat
    29161 gaaagcaata ttgaaatttg aaatcggagt gaagtaatca actgtataaa aaaatcggga
    29221 gatacacagt ttcgtgctat ctcccttagt agttttacgg tcatatcaag gctcattcaa
    29281 tcgtggtaaa ttcgtggtaa gatgagtgtt tttctatact tcaaaatgct tgaa
//
LOCUS       DAYYAG010000022        29100 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000022 DAYYAG010000000
VERSION     DAYYAG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.022"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHARQ_000852"
     CDS             complement(<1..1031)
                     /locus_tag="ACHARQ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8409299.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHARQ_000853"
     CDS             complement(1113..1802)
                     /locus_tag="ACHARQ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8409300.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHARQ_000854"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHARQ_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8409301.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHARQ_000855"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHARQ_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8409302.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHARQ_000856"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHARQ_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8409303.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHARQ_000857"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHARQ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8409304.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHARQ_000858"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHARQ_000858"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8409305.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHARQ_000859"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHARQ_000859"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8409306.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHARQ_000860"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHARQ_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHARQ_000861"
     CDS             complement(7636..8523)
                     /locus_tag="ACHARQ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8409307.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHARQ_000862"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHARQ_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8409308.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHARQ_000863"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHARQ_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8409309.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHARQ_000864"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHARQ_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8409310.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHARQ_000865"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHARQ_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8409311.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHARQ_000866"
     CDS             complement(10454..10957)
                     /locus_tag="ACHARQ_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8409312.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHARQ_000867"
     CDS             11554..12519
                     /locus_tag="ACHARQ_000867"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8409313.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHARQ_000868"
     CDS             complement(12618..13790)
                     /locus_tag="ACHARQ_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409314.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHARQ_000869"
     CDS             complement(13777..14922)
                     /locus_tag="ACHARQ_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409315.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHARQ_000870"
     CDS             complement(14936..15868)
                     /locus_tag="ACHARQ_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409316.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHARQ_000871"
     CDS             15985..17064
                     /locus_tag="ACHARQ_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8409317.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHARQ_000872"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHARQ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHARQ_000873"
     CDS             complement(17789..18016)
                     /locus_tag="ACHARQ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8409318.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHARQ_000874"
     CDS             complement(18119..20707)
                     /locus_tag="ACHARQ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8409319.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHARQ_000875"
     CDS             complement(20727..21386)
                     /locus_tag="ACHARQ_000875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8409320.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHARQ_000876"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHARQ_000876"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8409321.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHARQ_000877"
     CDS             complement(22159..23025)
                     /locus_tag="ACHARQ_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8409322.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHARQ_000878"
     CDS             23134..23775
                     /locus_tag="ACHARQ_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8409323.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHARQ_000879"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHARQ_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8409324.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHARQ_000880"
     CDS             complement(25216..26115)
                     /locus_tag="ACHARQ_000880"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8409325.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHARQ_000881"
     CDS             complement(26117..26914)
                     /locus_tag="ACHARQ_000881"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8409326.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHARQ_000882"
     CDS             complement(26892..27596)
                     /locus_tag="ACHARQ_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8409327.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHARQ_000883"
     CDS             27727..28302
                     /locus_tag="ACHARQ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8409328.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHARQ_000884"
     CDS             28420..29097
                     /locus_tag="ACHARQ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8409329.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6167 c         5230 g         9139 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAYYAG010000023        27963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000023 DAYYAG010000000
VERSION     DAYYAG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.023"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..534
                     /gene="mscL"
                     /locus_tag="ACHARQ_000885"
     CDS             73..534
                     /gene="mscL"
                     /locus_tag="ACHARQ_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8409330.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(593..1039)
                     /locus_tag="ACHARQ_000886"
     CDS             complement(593..1039)
                     /locus_tag="ACHARQ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8409331.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(1132..1377)
                     /locus_tag="ACHARQ_000887"
     CDS             complement(1132..1377)
                     /locus_tag="ACHARQ_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8409332.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(1492..2070)
                     /locus_tag="ACHARQ_000888"
     CDS             complement(1492..2070)
                     /locus_tag="ACHARQ_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8409333.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(2147..2677)
                     /locus_tag="ACHARQ_000889"
     CDS             complement(2147..2677)
                     /locus_tag="ACHARQ_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8409334.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(2681..3541)
                     /gene="rlmB"
                     /locus_tag="ACHARQ_000890"
     CDS             complement(2681..3541)
                     /gene="rlmB"
                     /locus_tag="ACHARQ_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8409335.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(3534..3929)
                     /locus_tag="ACHARQ_000891"
     CDS             complement(3534..3929)
                     /locus_tag="ACHARQ_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8409336.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(3926..5338)
                     /gene="cysS"
                     /locus_tag="ACHARQ_000892"
     CDS             complement(3926..5338)
                     /gene="cysS"
                     /locus_tag="ACHARQ_000892"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8409337.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(5335..5871)
                     /gene="epsC"
                     /locus_tag="ACHARQ_000893"
     CDS             complement(5335..5871)
                     /gene="epsC"
                     /locus_tag="ACHARQ_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8409338.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(5957..6196)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6193..7653)
                     /gene="gltX"
                     /locus_tag="ACHARQ_000894"
     CDS             complement(6193..7653)
                     /gene="gltX"
                     /locus_tag="ACHARQ_000894"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8409339.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(7823..8980)
                     /locus_tag="ACHARQ_000895"
     CDS             complement(7823..8980)
                     /locus_tag="ACHARQ_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8409340.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(9014..10384)
                     /gene="radA"
                     /locus_tag="ACHARQ_000896"
     CDS             complement(9014..10384)
                     /gene="radA"
                     /locus_tag="ACHARQ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8409341.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(10447..10974)
                     /locus_tag="ACHARQ_000897"
     CDS             complement(10447..10974)
                     /locus_tag="ACHARQ_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8409342.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            11204..12010
                     /gene="proB"
                     /locus_tag="ACHARQ_000898"
     CDS             11204..12010
                     /gene="proB"
                     /locus_tag="ACHARQ_000898"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8409343.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            12007..13254
                     /locus_tag="ACHARQ_000899"
     CDS             12007..13254
                     /locus_tag="ACHARQ_000899"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8409344.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            13330..13674
                     /locus_tag="ACHARQ_000900"
     CDS             13330..13674
                     /locus_tag="ACHARQ_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409345.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(13791..14117)
                     /locus_tag="ACHARQ_000901"
     CDS             complement(13791..14117)
                     /locus_tag="ACHARQ_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409346.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(14732..15934)
                     /locus_tag="ACHARQ_000902"
     CDS             complement(14732..15934)
                     /locus_tag="ACHARQ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8409347.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            16051..16566
                     /locus_tag="ACHARQ_000903"
     CDS             16051..16566
                     /locus_tag="ACHARQ_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8409348.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(16765..17211)
                     /locus_tag="ACHARQ_000904"
     CDS             complement(16765..17211)
                     /locus_tag="ACHARQ_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8409349.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(17258..18736)
                     /gene="msr(C)"
                     /locus_tag="ACHARQ_000905"
     CDS             complement(17258..18736)
                     /gene="msr(C)"
                     /locus_tag="ACHARQ_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8409350.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(19090..19719)
                     /locus_tag="ACHARQ_000906"
     CDS             complement(19090..19719)
                     /locus_tag="ACHARQ_000906"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8409351.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(19703..20167)
                     /locus_tag="ACHARQ_000907"
     CDS             complement(19703..20167)
                     /locus_tag="ACHARQ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8409352.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(20654..21922)
                     /locus_tag="ACHARQ_000908"
     CDS             complement(20654..21922)
                     /locus_tag="ACHARQ_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8409353.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(22153..22890)
                     /locus_tag="ACHARQ_000909"
     CDS             complement(22153..22890)
                     /locus_tag="ACHARQ_000909"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8409354.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(22957..23526)
                     /locus_tag="ACHARQ_000910"
     CDS             complement(22957..23526)
                     /locus_tag="ACHARQ_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8409355.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            23654..24526
                     /locus_tag="ACHARQ_000911"
     CDS             23654..24526
                     /locus_tag="ACHARQ_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8409356.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            24868..25740
                     /locus_tag="ACHARQ_000912"
     CDS             24868..25740
                     /locus_tag="ACHARQ_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8409357.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            25737..27695
                     /gene="nagE"
                     /locus_tag="ACHARQ_000913"
     CDS             25737..27695
                     /gene="nagE"
                     /locus_tag="ACHARQ_000913"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8409358.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
BASE COUNT         7980 a         5892 c         5147 g         8944 t
ORIGIN      
        1 aaagcgtata ttgttttttt attttttatt tttcgatata ctaaagacaa ataaaagaag
       61 ggagggtcat tcatgatcaa agaattcaaa gagttcatca tgcgtggaga tgtcttggat
      121 cttgctgtcg gcgttgttat tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa
      181 ggcttgatca caccactaat aggttggttt gtcgcaatga tcacaggtac ttctgattta
      241 gaaggagcac tttcaattct tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt
      301 aacgttatta gtgctatcat tacatttctg atcactggtt ttgtcttatt cttagttgtc
      361 aaagcggcaa accgtgcgaa gaatttacgc tccaaagaag agcaagtgga agtggaagaa
      421 actcctactg cagaagatta tcttagtgac atacgcgatc ttcttgccaa acaagtggca
      481 gaagacaata cacatccagt agagcaaaca aatctttccg atacttctaa ataataaaga
      541 atctaaaaaa aagaaagccg attttcagtg tcggctttct ttttttagat tcttaacttg
      601 ctacttgttc ctttttcatg tcgatatgtg gaatatcatc ttccaaatac acatctgaga
      661 ttgccttgaa tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga
      721 ttggtttttc tggaaaattt ttctggatca tgttaatcgt ttcttgaaca atctttcttc
      781 ctaaaccatc tttacggttg tcctgatgaa caaggacacg tccaaaatgg atactctccg
      841 cttcttcata tactcgcgtg taggcaagga tcttcccatc agtatcttga aaaaacgtat
      901 gccaagcttc tccatcaatg tcatcaactt cttgataagg acactgttgt tccacaacaa
      961 aaactgcgat tcgttctttg acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt
     1021 tctttacctg atattccact aagcaaacgc cccctgcttt tttattctta gcataccgat
     1081 tcgcacacaa aaaagcaaac gaaccttacg aatctcaggc tcgtttgctt tttaaactgc
     1141 ttcagttaaa aattcgatct caactgtccg agtaagtaca tctgaatagc tgtaagatac
     1201 tcgttcaaaa gaattttctt caggatccaa atcaacaacg aatactgaag gataggtttc
     1261 tgttaaaacg ccgcggcgtt ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat
     1321 tgggctgccg atacgacctt ctaaatcttt cttgattgaa gctaaagttg ttggcataca
     1381 gttcacctct actatcaact ataccacata tttttaaaat tttcaagttt accataaaaa
     1441 aaaaagaaat gcaataaaaa taaataagaa gctcattaag atttttctaa attattcctc
     1501 attataaaat ttcaagtgtt tttttatttt gagttttact ctcccgtaag cgctcattac
     1561 ttgcttgtga tccattgcta aacattcagc catgtctaca aaatcaacgc cttgcatata
     1621 ataatgaaaa attttagctt ctatttttga aaactggatt tcgcaatttt cgattgcttc
     1681 ttttaataaa agatgctgaa ttgctaaagg ttcttgcaat gtgatctctg ctaaaaagtc
     1741 tggtccttct gtctctattt tttggtcgat tgagacttca tctttttgat cacgacgctt
     1801 cagcgcgttc tgcttcctca acagagaata gatttgattg cttataacgc ttttataata
     1861 acttccaaag ctaacacctt tgcttttttc gtaagttctc aatgcctgat ataaagcgat
     1921 ccttgcttct tgttcccaat cctcagaaac ataatttctt aaataatagg tgtttttcaa
     1981 acgtagtaaa agtggaaaat atttttgata cagggcagca aatgcttctt cgtcaccatt
     2041 acgtgcagcg cgcaataact cctcagacat aaaataattc ctccataaat tcgtttatgg
     2101 aggaatctta tcatcaatga ttcatcgaac tgattagaac gatatgttat ttattttttc
     2161 tcattaaatc gtccaatttt tgggacaatt tttttaactg ctcttcattc catggagaat
     2221 tacgtcggaa atcaataaaa tgcatatctt tattttcttg ggctattgtt ttttccgttt
     2281 tttttatcgt cttatataat tcataagcag aagttcgtaa tgctccttgg gaaaagatca
     2341 tccactgttc tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct
     2401 gaccagcgat tctctcgata taactgtcag ccgtttcgtc ttctttcgta aaaacaacat
     2461 ccaactggta tttcttataa ttttgttgga tgcccggaac tagttgggca tcgaagacaa
     2521 cgatgacttc cattccttca tatttcgcat aatttgataa acgatgtaaa agagcttctc
     2581 ttgcatccgc cattttgtcc tgctttttaa gctggacaag ttccggccac gaaccaatca
     2641 tgttgtagcc gtctacaatc aacagctgtt ttttcatggt ctactccccc tgacgttggg
     2701 aaaaagcctg atacataagc aaaccagcag caacactggc atttaagctc tggacgtgcc
     2761 cggtcatcgg gatcgtcagc aattcatcca tttctttttt caatcctgga ctcattcctt
     2821 tgccttcatt accaatgact aaagcaattg atcctttggc attccatttt tggaagtttg
     2881 tgccggtcat atctgtgcca aatacccaaa atcctgtttc tttgagctgt tgcacagctt
     2941 gtaccaaatt cgtcacgcgt gcaacaggga catgttcgac tgccccagtg gaagtctttg
     3001 ttacgatagg tgtgattcca actgctcggt gttttggtat gatcacacca tcgacgccac
     3061 ttgcgtctgc tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa
     3121 tcaaaaagaa ggggtcttct tttttctctt ttgttcgttc aatcagttgg tcaagggata
     3181 aatattcata aggtgtaatc gcaagaacga ctccttgatg cacgccgtga tcgctcaaag
     3241 tttctaattt ttgtttagga acccatttga ctggcacagc ttgttcacgt gcgatttctt
     3301 tcagttcttc tactttttct cctcgtgaat cttcttgaag aaagagttta ttccctcgcc
     3361 cttgttgtag cgcttctact gcggcatggt gcccaaagac gaaattatct tctaatgctt
     3421 cttcattttg atcagaaact gactgatttc gtccagtttg ctttttattt gaatatctat
     3481 tttgtttttt gtcttcaaat ccacgtttcg attgatgttt gtttgttctt tctttaccca
     3541 tttttttcac caactttctg aatacaccag cggatcaatt cttccatccg ctcggtttgt
     3601 tttgtcaaat gcagatagcc catgagcgct tcgaaccctg ttgcgacccg atacgtcgta
     3661 acatcagcat tttttgcgct cgtatggctt ttagcatttc ttcctctgcg ataataaagt
     3721 tcttcttctt ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca
     3781 ttcgcagaaa cataattcgt tgccgcacga tgcagcttat tgggttttgt ttctccttga
     3841 tcaatcaggt ggtcacgaac atagatttcg taaatcgcat ctccaacata agccaaggct
     3901 aaaccgttca attgtgtgta atctctcatg cttctcttct ccatcgggtt ccttgcggtg
     3961 tatcttctaa taagatccct ttttctttca acaaatcacg aatttcgtcg cttcggacaa
     4021 aatcacgatt gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta
     4081 ataactcttc tgctgtaaat aaaataccga agatcgaaag ccattgcgag aataaagctt
     4141 ctccttgtgc caagacagcg gcagaaactt tcggctggtc tagataagtg ttcagccact
     4201 tagcaagctc atagaccact gtgatcccat ttgctgcatt aaaatcatcg tccatttctt
     4261 cgatgaatcg ctgttctaaa ttttctaaca aagcgatttt atccgcatca tctgccaaat
     4321 tctctgaggc agtttcttgg cggaatttca aattgtcaaa cgtattcttc agtcgttgta
     4381 aattggtggc tgcttctttc atcgtcgttt cactgtagcg gatcggacga cggtattgtg
     4441 tggtcgccat gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt tcatgtacag
     4501 tgatgaaatt ccctagagat ttactcattt tttcatcgtc ttcgccaatc gttacatagc
     4561 cattgtgcat ccagtaatta gcgaattttt tccctgtttt cgcttcactt tgtgcgattt
     4621 cattttcatg atgaggaaat tctaaatctt gcccgccacc atggatatcg atcgtttctc
     4681 ctaaatgttt tgttgccata acggaacatt caatatgcca accgggacgt cctttgcccc
     4741 atggtgaatc ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg
     4801 ggtcttcttt ttgctgctgt tcttcccctg tacgctgact tgctccgact tccagttcat
     4861 cgatggattg gtcacttaat ttcccataat tttcaaattt acgggtacgg taatacacat
     4921 cacctgaaga ttcataagca tagccttttt caaccaacac ttgaataaat gctaaaatat
     4981 ctggcatatg atccatgact cttggatgca gtgtagcagg ttcgacattc aacgctttcg
     5041 tatcttcttc aaatgctcgg atgaaacgat cagcaacttc tggtgctgta atacctaatt
     5101 cgtttgctgt acggatgatt ttatcgtcta catccgtgaa attcgatacg aaatcgactt
     5161 tatagcctcg atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat
     5221 ttccgatatg gatataatta tacacagtcg gtccgcagac atacataccg acatgtccct
     5281 tttgtatagg gatgaattct tctttttttc tggtcaatgt attgtagatt tttatcattc
     5341 tgatctcctc actttttctc ctttgattcg aacgactttt gctggtactc ctaccgctgt
     5401 agcgtcgtct ggaacatctt tcaacacaac ggcacctgca ccgattttgg cgttttttcc
     5461 gatcgttacc ggtcctaata tctgcgcatt cgcagagagc atggctcctt gtttaacagt
     5521 tgggtgtctt ttacccgttt cttttcctgt gccgcctaat gttacgccat ggaataaaat
     5581 cacatcgtcc tcgatttctg cggtttcgcc aatgacgatt cccattccat ggtcgatgaa
     5641 cacgccagtt ccaatcgttg ctcctggatg gatctcaaca ccagttagga aacgccagaa
     5701 ttgagcattg atcttcgcca ataaaaagag ccggtgctga tataaaaaat gagagaaacg
     5761 atgccaaaac aaggcatgaa aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc
     5821 agccggatcg tttctctttg ctgcatcaat cgctcgggta aaccaactca tgactttcca
     5881 ctcctttcgt aagaatttta tgtaggaaat catcttctta taaagaaaag aagcgccttt
     5941 taaataagtt tccttatcaa aagacgcttt ccgcgtggtt ccacttttct tcataatcaa
     6001 tgattatctc tgacagctaa cgcctgttat acgttcttgg ctactttgtt tcctataaaa
     6061 cagtttttcc tgttttctgt gtttcgccaa gaccactccc agaggcattt cggcaaccat
     6121 tcattgatac cttgcaccac ccggtatctc tcttgtaatg atcagctgct tacttttttc
     6181 tgttcatcgt gtttagttca gtacttgatt taaatgtgct aatgattttt cacgaccaag
     6241 caattcgatc gtttcaggca attctggacc atgcatttgt cctgatacag ctacacggat
     6301 tggcatgaaa agattttttc cttttacgcc agtttctttt tgaacagctt tgatggcagc
     6361 tttgacagtt ggagcatcta ctacttccat ttgttctagc tgtgctttga atgcgtggag
     6421 aacagtagga actgtttcac cagcaagtac ttcttttgct gcttcattca atacaggatg
     6481 ttcattgaaa aatagcgtag acagatcaac gatttctgca gcatagctca tttgaggttg
     6541 atacaggcta acgatcttct tcacccattc gattgtcttt tcatctgggt tttctgctac
     6601 acgtccatca gcgattagat aaggcagaca catgtcagta aatgcatcta gatccattgc
     6661 tttgatgtaa tggttgttta cccattgcag ttttttcgca tcgaaagctg cgggtgattt
     6721 acttaggcgt tttggatcga acattttgat caattcttgg tgttcgaaga tttcgtcttc
     6781 gcccacaggt gaccagccca ataatgcaat aaagttgaac attgcatctg gtagatagcc
     6841 taattcgcga tattgttcga taaattgcaa gattgattcg tcacgtttgc ttagtttttt
     6901 acctgtttca ctgttgatga tcaatgtcat atgtccgaag gttggtggtg tccagccaaa
     6961 tgcttcatag atcatcagtt gttttggtgt attggcaata tggtcatctc cacgtaaaac
     7021 gtgagtgatt ttcatcaaat gatcatcgac cgcaacagca aaattataag ttggcatacc
     7081 atcgcgtttt tggatgacaa agtcgccacc gatattatcg gattcgaacg aaatagcgcc
     7141 tttcaccata tctgtaaatg cgtattctgt attgcgaggc acacggaaac gtacaacaga
     7201 ttctagacct tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc
     7261 tgcgtaatga ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc
     7321 ttctgtgcaa tagcatttgt atgctcgatt gctagcaagc agctgatcaa tcagcggttg
     7381 atagatttct ttacgttcag attgacggta agggccgtat tctccaggat tttcaggaga
     7441 ttcatcccaa ttcattccca accaagcaag gttttccaac tggctttttt cgccatcttc
     7501 gatattacgt ttttggtcag tatcctcaat acgaataata aagtctccat cattgtggcg
     7561 ggcaaacaaa taattgaata atgcagttct tgcatttcca atatgcaggt gcccagttgg
     7621 acttggtgca tagcgtacgc gtactttcgt cattttcttc ctcttctttc aaactagact
     7681 ttgactaaca gcaaaaattc cgtcagttac ggcttctcaa atatcattta tttaaagcca
     7741 ctcagcacaa attttacctt ttctaaggta aatagtaaag ccaactggtt gaatttcctt
     7801 accagttggc tgaatttctt ttttattttg attgtttagt ctttttctct gtctcttttt
     7861 tattgtcgat tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg
     7921 tttgaagggc acttgttaca acgacttcga tacgttcatt catgtaatgc tgtccatctt
     7981 ctacgacaac catcgtgccg tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg
     8041 cttttactac tagaacattc atcgtttcac cagggatgac gactggtttt acggcatttg
     8101 ccaacgcatt gatattaaga acaggtacat tttggaattc agagacttta ttcaagttat
     8161 agtcattggt tacaatgaca ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta
     8221 cttcactgat atcttcaaag tctccatcgt acatttcgac agatataccc tcttcttttt
     8281 gcaaggcatt taggatatct aatccgcgac gaccacgcac acgtttcaaa ctatcccctg
     8341 agtccgcgat gtattgtagt tcgtaaagaa caaaattagg gatcatcaag gttccttcta
     8401 agaatccagt ctttgtaata tcataaatcc gaccgtcaat gatcacgctt gtatccaaga
     8461 ttttatattt atggaaattt tctcctgatt ttcgttctaa cagttgactc tcaacttgac
     8521 tagctgtttc ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc cattcttcga
     8581 ttcgtgtcgt tcccattctc aatcccagat agcctaagat gatcatcagt aaaatcggca
     8641 atacactatt gacgaatgga atttccatat tgtaaagagg ggtcgagatc acaacaccta
     8701 aagtcaatcc gatgatcgca ccgatcgcac cgaagagaag ataagtcaag ctgacctcac
     8761 ttagtttttg ttctacttgt ttgataatag ctgagatatg tttggccaac agcaaagata
     8821 ataaaaagaa aataagtgcg cctaacaggc tgttggtaaa tgtattattc agccatccgt
     8881 ttgactgttg accgattgcc tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc
     8941 cgacaatgat catcagcaaa gtaatgacac gtttttgcat cgtttttcct ccgtttcgac
     9001 aatcatttat tagttagcgg aacacacgct tcaacgtttc agccaaagtt gctactccaa
     9061 ctatctcgat tccttttggc gcttcccagt tacctaagtt gtttttaggt aggtatactt
     9121 ttgtgaagcc gagtttttgg acttctttga ctcgttgttc aatatgactg acacgtctga
     9181 tttctccagt cagtccaatt tcaccgatga aacattccgt tggctgtgtt cctttttctt
     9241 tatagcttga agcaatactg acagcgatag ctaaatcaat cgctggttca tttaatttta
     9301 ccccacctgc agcttttaag taagcatctt gattctgaag aagcaaccca gctctttttt
     9361 ccagcactgc catgatcaga gatacccgat tgaaatcaag acctgttgtt gttcgtttgg
     9421 catttccgaa catagtaggt gtgactaatg cttggatctc taccaaaatt ggacgtgtcc
     9481 cttccattgc cacaacgata gcagagccag ttgcaccgtc taggcgttct tctaagaaaa
     9541 tctgtgaagg attagcgact tcttccaatc catgctcatg catttcaaaa atcccaatct
     9601 cattcgttga gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt
     9661 caccttcaaa atataacacc gtatccacca tatgttctaa cattcttggg ccggcgatcg
     9721 agccttcttt ggtaacatga ccgacgataa aaatggctat cccatttgtt ttagcggttt
     9781 tgagtagttc tgcagtggtt tctcttactt ggctgacact tcctgccaca cttgtgatgt
     9841 ctggctgcgt catcgtttgg atggaatcaa tgataacata atcaggggag atatgttcga
     9901 tggcacgact gatttcgttc atatctgttt cagcgtatag ataaaattct gtgttgatac
     9961 ttccgaggcg ttccgcgcgt aattttatct gctctgcgct ttcttcgccg gatacatata
    10021 agacttttcc gcctatggct gctaattgct gagaaacttg taacagcaaa gtggatttcc
    10081 cgatacctgg atcgccacca atcaagacca tagaccctgg caccacacct ccgcctaaca
    10141 cacggttcag ttcttctaat tttgttttga tccgaggttc ttttttaggg acgacgtctg
    10201 cgatttttgt tggttttgct ttttctcctg tcaaacttgt ccgagttctg cggtccgttg
    10261 tatcttgttc gatttcttcg accatcgtat tccattttcc gcaatttggg cagcgtccca
    10321 agaacttagg cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg
    10381 ccattgtacc gcctcctttt atcctaatca tgagacatta aatcaagctt aagtatcttt
    10441 aacgttttat ttactggaag aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg
    10501 atctgctttt aaaaacggca aaaagatccc ttggccgata cgctcgcctt ttttgatcac
    10561 tacatccttg aaaccaaagt tggtgaattg gaacatgata tggccttcat ttgcctcatt
    10621 attgtaataa tcactgtcaa tcacacctac tccgtttgtc aacactaaaa accgcttcaa
    10681 cggattgctt gaacggttag caagctgcaa aaattcatct tctcccatgt aagctttgat
    10741 tccagtaggc acgagggttg gtttcaattg tttttcattt tctgatacat cttttttcaa
    10801 tccatctgcg actgtttcca accccttttt ccagcctgct ttccagatac ttggaatcac
    10861 gacctcttct gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc gctgcggtag
    10921 attgatattt tgatctttat atttagaaat aacttcaaat cctctttttc tcaccggttt
    10981 tcctccctaa ataaactact cctattctaa catatccgag gctgtaaaag actttcttta
    11041 caaattcttc aagaaattct gctatcttaa agtaaaccat taattcgttc tttttccaaa
    11101 cgactgcaag ttgtacaaaa aaactatttt ttacatgact ttactcgtca aaacaagtag
    11161 aatagaacga aattaatgag tggatatttg attgaggtgt cacatgcgta aagaaattaa
    11221 aaatgcaaaa cgaattattg tcaaagtagg aacaagtaca ttgatttatc ccaatggaaa
    11281 tatcaatctt gcggcaatcg atcagttagc ttttgtcctt tctgatctta gaaatcgggg
    11341 aaaggaagtg atcttagtct catcaggagc tgtaggcgtt ggtatgcata aattacattt
    11401 gtcagaacgt ccgactttga ttcctgaaca gcaagctgcg gcggctgttg gtcaagccga
    11461 actgatgaat atctataatc aaagattcag tgtatataat caacagacgg cacagatcct
    11521 cgtgacaaga gacgtggtag attatcctga aagtcgtaaa aatgtgagga atacattgga
    11581 acagcttttg attatggaca cgatccctat cgtgaacgaa aacgatactg tagcagtgga
    11641 tgaattagat catctgacaa aatttggcga taatgatcag ctgtcagcca tcatggcaaa
    11701 acttgtcgcg gcagaccttt tgatcgtcct ttctgatatc gacgggtttt attcagataa
    11761 tccgactaca aatcccgagg ctcagatgta tcgctatatc gataaaatcg atgaaaagat
    11821 tctggcacaa gcaggtggtg ctggtagcac atttggaaca ggcggtatgc agagtaaatt
    11881 aaaagccgct tctaggatat ttgaagcaaa cagcgcaatg attttagcaa atggaaaaaa
    11941 tccggcaatt atttttgata ttcttgcagg aaaagaagta ggtactttat ttaaggagga
    12001 cttgaaatga ctgacttgat gcaattagga caacaggcaa aagatagcgc gcaacaacta
    12061 gcacttatgc ctacaaaccg caaaaatgag ctacttgttc aaatggcaca aaccatcaaa
    12121 aagaatcaac aagcgatcat tgaagcaaat cataaagact tagaaaaagc agtcgaaaac
    12181 aatatcagtg aaacgatgca agaccgcttg cagctgacac ctgagcgaat caatgccatg
    12241 gctacagaga tcgaaaaaat tgcttcgtta gacgatccta ttggaaaagt agatgagatg
    12301 tggacgaaca ccgacggatt gagaatcggc aaaaagcgcg tgccactagg cgtgatcggc
    12361 atcatttacg aatctcgtcc aaatgtcacg acagatgcag ctagtctagc attcaaatca
    12421 gggaatgccg ttatccttcg cggtggcaaa gaagcattct tctcgaatca attattagtc
    12481 caattattgc agcaagtact ggtcagtgct ggcgaatctc cttatgccat ccagtttgtc
    12541 gatgatactt ctcatgaaac tgcaggacag ttgatgcgat tgacagaata cttagatgtg
    12601 ctgattcctc gaggcggagc aaaattaatc caacgagtca aagaacaggc aacggtacct
    12661 atcattgaaa caggtacagg aaataatcac gtatatatcg ataaagaagc acagttatct
    12721 atggcagtga acatcatcgt caatgcaaaa gcttcccgtc catctgtctg taacgcggca
    12781 gaaacattgc tgatccattc tgagatcgca ccttttttct tgccagctat tgaaaaagaa
    12841 ctggtagaac acggggtctc tttacgtgcg gatgctcgtg cgttagaata tctagagacg
    12901 gctgctttag cagaagacac agattgggat actgaatatc tagattatat ccttgctgtc
    12961 aaagtggtcg attctttgga tgaagcaatt gcacatatca atcggcataa tactaaacat
    13021 tcagaaacga tcgtgacaga tagttatgcg gctagtcaac gtttcttaaa tgaagtggac
    13081 gcggcggctg tctatgtcaa tgcctccact cgttttacag acggatcggt atttggtttt
    13141 ggtgcagaga ttggtatctc tactcaaaag ttgcatgccc gtggtccaat ggggttaaat
    13201 gaattaactt ccacaaaata tatcatttat ggcgatggac aaatccgcga ataatgtttc
    13261 gtgtgaaaca ttattcccgc cttttgcctt ttttctgtaa aggttctatc caactagaaa
    13321 ggtgtcaata tgtttgaaat tttaatgatt ttttttattt accttatttc tctgaatatc
    13381 gctgcttttc tgggagtcgg tattttatcc cttttctttc aattcaaaaa acgaagcata
    13441 gggagtcaac gggaaaaatg gtcgcagtat tttgacaaaa tcgggcaaaa agggctggtt
    13501 acacgcttgc acattagtta catggttgca ttatgcttat tagcgacaat caattactat
    13561 tcttttttcg atcattcaat cgcttatacg attacgctac tgattgctgg gatttttcac
    13621 ttatcttata aatatcagct gaataaaaac cacttgaatc ggactttccg ctaaaaaata
    13681 tccgtacgat aagtagagtg ccttgaagat cggcaaaaaa ctgccggact ttttagaggc
    13741 tgtgacaaag attttgtcac agcctctttt tccatataaa cggcgttcgc ctaccagctg
    13801 cctggtaaat ctgtttcctc ttcgttaaga agcgaaaatc cgtcaaatgt ttcgccatca
    13861 taataacgaa gcagctcatc catggacaag ttcatcgttt tttctttttc ttcttccgct
    13921 gattcagcca gtagctgttc ccgatacacc tgatacatac cagctatttt ttgctgcatc
    13981 gcttttctag tttcaccttc cgcattgatt attggcaatt ctttgatata gccacgataa
    14041 atcaaccgat catctgaaaa ttcaatgggt aattcaacga gtgtcaaccc gacttcctga
    14101 aaatattctt ccagcatcta ttatcccccc attctttttc ttaaatgaat tttagcttac
    14161 ctactgaaaa ttttccagtt tcattatttt gagaaaaaaa gggaatgtga caaaaatcgt
    14221 gtcacatccc ctttttccga ataaatggtg ttcttctgcc aactcctcaa tcataagtca
    14281 caatattcaa caaacatgag aaactgtttt ttgaaattgt tccttgtgat tcagagttaa
    14341 acgacagtct cacaacctct tggtaaacgg cgttcaatca gctgaccctc ggtattagct
    14401 caaaaaacgg taaaatatga gaagctattt ttccgttttg tttctctaat acttaggata
    14461 aaacgctgat tttacaacct ctgttaaacg gtgttcaatc agctgtcctt cggtattagc
    14521 tcaaaaaacg gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggaca
    14581 aaacgctgat tttacaacct cctgataaac ggtgttcaaa agcttgcatc tgcggtaata
    14641 agcccaacca aacgaaaaat atgagaagct atttttgttt ggctgttctt atgacttgat
    14701 gcaggacagc tttttcacaa cctctgattt cttattcttc ttcatttgta gcaaaataat
    14761 tacggtgata aatgatacaa catacactga taagaacaat ggcacctgca aaataaaacg
    14821 catagctttc accaatatgt tcaccaatca cacctgcaat aaatggtgct aaagctccac
    14881 cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt tcgttgcaga cctggtattt
    14941 ccatagcaat cgtcgtcaac agtgtattga taattccttg gaaaaatcct gctaacacaa
    15001 tgctgatcgg tacaaggact ggcatagaag ttccagctcc taataacagc aaacaaataa
    15061 tgaataagac aaatgctgct aaaattgtcc gataagtcgt aaacttccgc tcaagagccg
    15121 gtgcaacgaa aatcgaagca atagccagaa gcacacccca gccaaaaaat acaaaaccga
    15181 cttctaattc agaatagcca atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc
    15241 cgaaattata taacaatgcg attagtccaa ctgaacgaag tttatgattg cttaacgctt
    15301 tgacaccttc ccaaaggcct acttttctct ttggtttctc agtttcaggt aaaagtaccg
    15361 ctagggctat aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga taacgccatg
    15421 aaccactgcc caagacccca ccgactagag ggccaacagc cattccgcag ccaagtgctg
    15481 cttcatacat catgattgct ttttctgtgt taccaactaa aatactgacg atcgttgcta
    15541 aagcagtaga gacaaataaa gcattcccga caccccagcc ggcacgtaac ccaatcagca
    15601 ttccgatatt cccagataat ccgccaagac cagcgaatac aacgatgata aacaatcctg
    15661 tcatcaatgt ttttttcaaa ccgatccgac tagataagaa acctgtaaat agcatcacca
    15721 caccagtgac taacatgtaa ctagtaaata acagggtcgt ttcagctggc gtagcgttta
    15781 actgtgcagc aatcgttttt aagattgggt ctactagacc cactcccata aaagcgatca
    15841 tacaggtagc tgctacaact agcgctgaac ttttcccctt tgtttcttca tttgtttgtt
    15901 cttgtatatc tatttgattc tccaatattt ccatcccttt ctctctatta gcatcataca
    15961 tgatttattt tacatgtcaa tatgcacatg taaaacgtgg aaactttact gtgatataat
    16021 aaagaaaata agacgagagg taggattttt atgaatactt tagcctatat attattatgc
    16081 gtgcttttat ccaaaccacg ttctgggtat gaattgaagc agctgatcac aattttttgg
    16141 gaagcccatc acagtcagat ctatacaacg cttgccaagt tagaccagca gggttatgtg
    16201 accgttttgg acaaagacga gcactctcaa aaaaagatct accacttgac tgatgaagga
    16261 cgtgctcttg tagaggaatg gatcaaagag gaaactcccg ctcctacgca aaaagatgaa
    16321 tttctagcaa aaatctatgc tttttcgacg ctcgataaaa caacagctac tggactgcta
    16381 ttcactagag aacaacagct gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac
    16441 ctctcgctag acaagaaaca agaatttgga cgttatgttg tgatccagcg cagaattatg
    16501 ctttgtcagc aggaactcca atggtgtcag caagtaagag aacaaatgca gcagtttttt
    16561 gagtaaggga taaacagaac ctttctagaa atatctattg aagagcaaat tatcttcaat
    16621 aaaagagcaa tacagttgga aaagaaaaag cgatccgaac gacaggccaa aacctatcgt
    16681 tcggatcact atttttctag aactgcattc aagagactat gacaaaaagc atgccaaggc
    16741 cttttttcaa ataacagtgt tccatcattc attactttga attgctttta gcacacttct
    16801 tgaaagcggg ccaacgatga tcaactgcaa cggtaaagcg gcaatcacat tcattttcca
    16861 agcattcata taagcagaga ataattgatc aggtattcct tgttccatga tcacaccgaa
    16921 taaagacata catgtcacca ttcctaaaac catcaagcat gaaatagtta agatcatttg
    16981 gaccttgctt tctggattta ctggtagttt aaaggcaatt tttttcgcca gcacaccgat
    17041 gatgaagaca tccaaaatga aagccaccac gaatccaggc actagaccaa tcaacaaact
    17101 tgataaatga agatcttgat gcagccataa attatacgcg ctcatcccca agaccatcaa
    17161 aaaacacatc attgttgtaa aaattattcc ttctttttta tttgtaggca atctgtacca
    17221 ctcctttaaa tttgtcacga aaatcatcgt agcacattta aaaatttctc gtaagtactt
    17281 tttttatttt ctgccctttt atttcataaa cttcatcagc cgtacgctcg acaaagtaag
    17341 aatcatgaga agtaaacaaa atcgttcccg gatatatttg cattagcttc tctaaagctt
    17401 cgatcgttgc catatcaata aaattagtcg gttcatctaa cagcaacaca ttacttggct
    17461 tagtaaaaag caaagcaatc gtcagacggg tcgcttctcc cccacttaat gtacataacg
    17521 gtttcgtcaa agtttcctga gcaaacccta agtgattcag caaactacgg acaagcgatt
    17581 ctgaaaactc tgtttgtctc atgacaaaac gaatgatgga ttcttcagat gtcatttgat
    17641 aacccttttg ctgatatact tgaaagctta ctttcggaga gagaaggatt ccttctcctt
    17701 gtttgcggat atgttctaat aaggttgtct tacctgaacc attttcaccg acaatcgcga
    17761 tttttttacc taaagaaaac tgaaaatcac catttaccag taatgttctc ccacttcttt
    17821 ccaattggac attttgtccc atgatgggat agcggctgtg gagttcaaga gattttggta
    17881 ctggaaaacg gatttgtttc atctgctgcg gcttggttgt ttctcctatt ttttggagtc
    17941 ttctctctaa tgttttcgca ttcttttgga tggctttttg tacactgtct ttttgtttag
    18001 aggaagacaa ccgatccagt ctgatttgtt gctttttctt tttactggac actttttcca
    18061 acttttccgc ttgttttttc ttttcttgga tcgatttttc caatcgtttt ttctctttca
    18121 tgatcgactc tgcttctcgt agctgagtct ttttctccaa ttccttttga gtgagatagg
    18181 cactataatt ccccgaaaac tctcggattt ctccgtcttt tacttcccag attttcgatg
    18241 ctagctcatc tagaaaatag cgatcatgac taacaaaaac cagcgtacca taataataac
    18301 gaagctcttc aatcagtcga tcaatacttt ttctatctag atgggttgtt ggctcgtcta
    18361 gaagcatccc ttgttcataa gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc
    18421 ctccgctcca tttccgctct gaagtttctt ccagtcttaa ctggaaatga ctggctaatt
    18481 ccaaatttag gtcctttgct tcagcgagat aggttaattg aggcaggtag ttgaaggtga
    18541 tctctgtttg gattttccca tgatcgggaa aaagttcacc ggctattagt ttgagcaaag
    18601 tcgatttacc gacaccattt tctcctatga tccctatacg atcttgctga tagacacgaa
    18661 gagaatcaat agatagttct agctggttgc caaagctgac ttgtagattt gttatattta
    18721 ctgctaaatt ttccatatgt tttacactcc tatttcatgt aaaacaacaa ttatcgatac
    18781 taaaaaagag cagaaaaatc tgtttgcaaa aaaactgcat cacatctttt cctactctga
    18841 acgaaataac tatgactaaa tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt
    18901 tcgaaaaaat ggacatactc catccatgaa attgttgttt atacatgtct tttgaattta
    18961 tttagttgtt attcaaaagt tcgaaacgta atttcatcga tgcagtcatt tttccgacct
    19021 ccttttttat taggttacca cacacgattc aaaaaaagca atagctactg aatagtaact
    19081 gaaaaccttt tattcgaaca tagtgatctt ttctggtgct aaacgaacag actcgtaacc
    19141 ttcttctacc gcttttccta ttgataaaat aataactgga acataacggt ctttatccaa
    19201 accaaatgcc tctgccaact gatctgcttc gaatccacca attggatttg tttcatagcc
    19261 atgatcacgc gcaactaaca tgaattgcat agccgctaag ctagaatcga ctttgactac
    19321 atcgttcatt tcttctcttg aaaaactctt gtaataagga atgatcgcag ctaattgttg
    19381 atctctgact tcttttggca tcttccctga ttcgtaagct tggttgtaga tttcttcgcc
    19441 atattcgtaa cattgcatat cgccaaaaat catgaccatt gcagaagaag tatcattttg
    19501 tctcgtattg aaacggatca aaggacgcaa gatttctttt gcttcctcgc tttcagcgac
    19561 cacaaagcgc catggtttca tattgacaga agaaggtgca gtcgtcgctt ctttgatcat
    19621 ttccagcatt tcttcgtgtg ggattttgta tgtttcatca tatacgcgaa cggatcttct
    19681 accagtcata attgttgaaa aatcattttt ttgttccatt attattaacc tctttcgttt
    19741 ctgttgttat attgcaaagt tgggtattca atttatctaa aagtgttaga aattgtttca
    19801 tttctacatc gctgaaagcc ggggatagca cggaacctgt atctttatgg tccatttcac
    19861 aatgcttgat tttcttctgg gctttttctg ttagcgctac atacacttcc cggttgttct
    19921 gaggatttct ctgacgcgaa acatagccat ttgcttccaa taccttcaga tgacgagtaa
    19981 ctgctccttg atcaatctgc aacttttctt gcaaactgct ttgtagcaat ggattttctt
    20041 ctttcagtct catcatgatc tcgtatctag taagacttat accgatattt tcttcaaaga
    20101 tctggacgat tttttgatcg gctaatttga tttgatataa aaggtgacta atctcttcta
    20161 aagacataat ttcctccttt ctaataattg actcgtcaac gattgacttg tcaataatag
    20221 aataaaaaaa gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga
    20281 aaagtataga gaaaagaaat tttttgtaga atgaacggaa aaaatgataa agaaagcaca
    20341 acgctaaaac caagattatt gattcttatc aaactataga agatagctct tctgcagtag
    20401 ataattttac accgccaaaa atttccgaaa tcgccttttg caagaatccc gtcgaatatt
    20461 agaaattgga tcagtatgct taaacagtaa taaaaaagtt atgacaagat gttgtcacaa
    20521 ccttttttga gtaaacggtg ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca
    20581 aaaaacatga aaatctgttt ttgtttggct gttcttacta cttgatgcag ggcagctttt
    20641 tcactacctc tttttaataa ccataaaatc ctattccttc atatctccag cccatcggtg
    20701 ttagccaatc tctttcctta gcactcaagg tatagtgatg ggtaccagtt ttttcatttg
    20761 gattaaatag tcgataaatt ggcttggaac caccagaata aaaacttaga ccttcataac
    20821 gccaaccgat ttttgttaaa gagttgatct cagattgtgc cattgtgtaa tgatggtctc
    20881 cactgtaagg attgtataaa cggtagacag gttgtccgct gtttggtgct tgccaagcaa
    20941 tcccttcata ttcccagcct acagttggca gataattttt ttctccggca ttcaatgtat
    21001 ataaatgttc tcctgtatga cggttataca gacgatacat cggagaagtt gaagttggtg
    21061 ctgtatcata ctgtgtcaga ttataggttt cgattacatt gttcaatgta gaaccataat
    21121 taggcgcagt ggcataacga cctgttagcc aagccgtagc atctttgtaa gagttcgtat
    21181 tgcttttcca tgcaccggaa tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata
    21241 ccgttacgtt atcttgcaat gattctttgt acgaaggata ttttctaaaa gcggcattca
    21301 ccgtaatcca ttgcccattc acatattccg aagtaggcat cgtaacagat tggccattat
    21361 agcttccttt aatcccaaat agattgtaat taggggcaga agccaaagta ctgttccccc
    21421 agccactttc aacgatggct tgagcaatca tgactgatgc atataagtca ttggcacttg
    21481 ccaaactttg tgcagttgag gcaatactat caatgaacgc ttgttgagaa ttagcagatg
    21541 agcgagcata aaaagcatag ccagtatagt tttcgaattc agacaaatct gcttgatggg
    21601 tatgtgtcaa tgtttgccca tctgtcaatt ggttcgtgat ttcttctgca ttctctggat
    21661 tgttctgagc ttctgctgat gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg
    21721 ttggttcttc ggttgaaatt gtattttctg agacagtagc tgactgcaaa gtactttctg
    21781 tatcgttttc aattcctgtt tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg
    21841 attcttcagc tagcactgtt gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa
    21901 ataataaatt ctttgctttc atcttttttc tcctttgaat tatgaaactt ttcctaaata
    21961 acagtgtaac taaaaaagta aaagcaagtc aatgtccata tttaaataac atttgtttct
    22021 ttttaataaa tttgcttttt ctgcataaaa agacgtaaat gaagaatatt cttctccatt
    22081 cacgccttaa attgatggtc gtttgaataa ctatttaaca agtaattctc tctggatatt
    22141 atccgttatg gattatactt cctgtgtcgt cggacggata atcatgttgc tgacagacat
    22201 ccggtctggc gtttctatcg caaatgctac agcagaagct atatcttcag atgttaatcc
    22261 ccattctttt tgcgcttcat gaagttcaag agcagccttt ttgtcgctga tggtttgata
    22321 taattcagtt tgaacagctc ctggagagat gatcgtagat ttgatgttat tttgccgttg
    22381 ctcttgacgt aatccttcca tgattgcacg gacagcaaat tttgttccgc aataaacggc
    22441 ggaatcagga taaacaacat gtcccgccac agaatcagtt gaaatgatat gcccagattt
    22501 ttgtttttcc atgattggaa gtacggcgga aattccattt aacacaccca tgatgttgat
    22561 atcaagcata ttttgccatt catcacggtg accttcaact aatggagcgg ttggcatgat
    22621 tcctgcatta ttgtacaata catcgattcg accgaacttt tccatcgcta aatcgataac
    22681 tgcttggact tgtgcaaaat ctcttacatc tgctgtttga atatatagct cagcttctgg
    22741 taaagattct tttattgctt ttaggcgatc ttctcggcgt gcagctataa ctagtttagc
    22801 ccctttttct gctaatagct tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat
    22861 gacaatcact ttttctgata aatcattcat tctattctcc tcctgttttg aaaaatctct
    22921 tgcttatact cactatctct tatttcatct tactgttcaa ataatccgtt cgacaggttt
    22981 gacgattttg gcagggattc ctgcaacgat ggtattgtct gggacatctt tcgtaacagt
    23041 tgcattcgct gcaacaatgg cattctcgcc aatcgttacg cctggcagaa tagttgctcc
    23101 tgctccgatc caagcatttt tcttgatgtg gattggcttt actgacaagc ctcttcgttc
    23161 ttttggatcg gttagatggt tcaccgtgat gaggttgaca cgaggaccaa tcaatactga
    23221 atcttctaaa cagatgccac ctaagtcaac gaacatcact tgacgattga taaagacatt
    23281 tttccctata ctaatatgtt tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt
    23341 atcatctact ttgtcactaa tgatattttc tagccgtttg cggatatctg ctggttcatg
    23401 gtacccagta ttcagcgctg ctaaaggctc ttcgttttcc gcttggatct gatggatcac
    23461 ttcaaagagc ggatccgtta ctcgaatctc tttattgacg atttgttttt cgatgaggct
    23521 tgtcattttg ctccctcctt ttatccttac tttataacgg acggcttatg atgtctaata
    23581 cttataaaac atacttaact attcctttta actatactta gtggtatgct tttttaaaaa
    23641 caggagggaa aaaatggata ttcgtatact gacgtatttt ttagcagtag ctagagaaaa
    23701 aaatatcagc cgagcagcaa aatctctgca tatcacgcaa cctactctct caaaacaact
    23761 gaaagattta gaagaagaac ttggtgtgac acttttcact cgaggctccc gagagataca
    23821 gttgacggat tctggtcgat atctttattc aaaaggaaaa gaaatcttgt cgttggtaga
    23881 aaaaacggcg aacaatttaa caaatgacgc aatcatctct ggagaaatct acattggcgg
    23941 aggcgaaact aaagcaatct ctttcatttc tgatagcctt cattcgttga tcaaggcgca
    24001 tcctgatatc cagtttcatt tatatagcgg gaatgcagat gatatcatag acaaactgga
    24061 tagcggatta cttgattttg gactcgtaat cgaaccgaca aacaaacaaa aataccagtc
    24121 atttcgactg ccagcccaag atcgttgggg acttttgata catcggaatc atcctttagc
    24181 aaagcaaaca gtcatccatc cgcaggaact gaaaactgtt cctttgatca tttcccggca
    24241 aacgtcagtg gacagtcaac tagctgaatg gctaggtttc agcatagatc agttatcgat
    24301 cgtcggaaca tataacttat tgtacaatgc ttctttgatg gtcgataacg ggacttttgg
    24361 tgcattagct ctagatggga tcatcaatac ttatgggacg gaaaaagtat tcatcccact
    24421 cgatccgcca ttacaagctt cactgaatct catttggaaa aaagaccagc ctctttctcc
    24481 tgctgctcgt aaatttttag atgtcctaac ggagaaatac agttaagtag agtttttttc
    24541 cttcgctttc agctttttaa caacgaataa gttaagcaaa agatccaatg attaaataac
    24601 ataagtttct taatttgttc cttcctttta tttctgataa ttccagtaaa ttactataat
    24661 tataaaaaaa gaattgacaa aacataactg tctatataaa attgattacg ttaacaataa
    24721 ttcgtttcat gtgactagat catactaggc atgaaaaatc tctgaggaac atggtttccc
    24781 taggaggttt ttcgtgtcta tttttgtttg gccgcaatca actggtctag caaacgtgaa
    24841 aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca
    24901 gtattggtag atgacggtgg acaggaaaaa gtagctattg gaaaagggat tggatttgac
    24961 agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac ggatatttgt catggaacca
    25021 gaaggtcaaa tcaagttgca aaatttactt aatcagattg atgaacgctt tttatttgca
    25081 gcagaaacga tcattgatca tgcggaaact gttttgatgg aaaagttgaa tgaacatgta
    25141 ttgatcgctt tgaccgacca cattgctttt tctgcggaga acattaataa cggtatcgtc
    25201 atccgcaaca aacttttaag agaaatcgaa gtgctttaca gtgatgaatt cagtattgct
    25261 caatgggcag tagagtatct gacaaaagag cttgggattc catatactta tgacgaagca
    25321 ggatatatcg caatccatat tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt
    25381 atccgtgaag tgacgattgt atctgacatc atccatttag tggaatcaga attggaaaca
    25441 gatatccatt cagaaccatt ttcactgaat tattcaagac tggtgaatca tttacgactg
    25501 cttctacaac gaacccatgc gcaacaatat gctgttttag acacggaaat cgtagaaatg
    25561 gtcaaacgaa aatacccaga aagctataag atttctaaaa aaatccgtgt gttgctgacc
    25621 aaagaatacc agctcgcgat cacgaaagaa gaactgggtt acttggccat ccatatcgag
    25681 cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc atgaggagga aaaaaattga
    25741 aagcatatct acaacgaatg ggacgttccc ttcaacttcc agttgcggtc ttacctgctg
    25801 ctgctttact agtagggatc ggaaactggt gggcatctta tagtaacgac attgtcgctc
    25861 attttttaca agctggcggt aacgcagttt taggtcaatt acctttgctg tttgccgttg
    25921 ggttagcact agggatgtca gtggacaaat caggtgctgc tgccttggca ggtttggtcg
    25981 catttgaagt tccagtaaat gttttaaaaa ctgattcagt agctacttta ttgaatatca
    26041 aaccagaatc agttgatcca tcttttgctc agatcagtaa cgtatttatt ggtattattg
    26101 ccggattgat tgcggcagct ctttataatc gtttccacga agcaaaatta ccgatggctt
    26161 tatctttctt tagcggcaag cgtctagtgc caatcttggc tgcctttgtc atgttgctga
    26221 tcagtgcagt gctactgctc gtatggcctc cggtttactc tgctttagtt tcttttgcta
    26281 aattcatttt gagtttaggc tggggtggtg caggtcttta tggcttcttc aaccgtttac
    26341 tgatccctac aggattgcat caagcaatga actccgtgtt ctggtttgac gtagcaggaa
    26401 tcaatgatat tggtaaattt ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc
    26461 aagctggatt tttcccaatc atgatgttcg ggcttccagc aggagcatat gctatttaca
    26521 gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt gatgttagca gcagcttttg
    26581 cttcattctt tactggtgta acagaaccac ttgaattctc atttatgttc gttgcttggc
    26641 cattatatgt cttgcatgca attttcactg ggatttcttt agcagtgagt gcattcttcc
    26701 attggacagc aggatttgcc tttagtgccg gatttgtcga ttacttccta tctttgaaaa
    26761 atccgattgc gaatcagcca ttgatgttga tcgtccaagg attagtaaca gcagtgattt
    26821 actactttgg ctttaacttt gctatcaaaa aattccatct gatgactcct ggccgcgagg
    26881 aagcagatct ttcagatgac gatactgcaa cgactaattc agacaataaa tatgcggcac
    26941 aagcaagcaa aatctacgct gcattaggcg gagccgacaa tgtaacttcg atcgataact
    27001 gtactacacg ccttcgtttg caagtcaaag atacaggcct gatcgatcaa aataaaatca
    27061 aagccactgg cgtacctggt atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag
    27121 gtacagaagt acaattcgtt gcagatgaga tggcaaaact acatggtggt gctgcaacac
    27181 gacctgcaca aacaaacacc gtggttaaaa cagaaacatt tgccggtgga acgatcgaac
    27241 aagatattta cgctatcgca aatgggaaac tgattccaat cactgaagtt tcagatgatg
    27301 tattcgcgga aaaaatgatg ggtgacggct atgctgtatt accagaaaat ggagaaatct
    27361 tctctccaat cgctggtacg atcacaaaca ttttcccaac gaaacatgct gtcggaatcc
    27421 aaacagatgc tggtatcgaa gtcttgcttc atatgggcat caacactgtt gacttaaaag
    27481 gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg
    27541 ctttagttga tcttgccgct attcaatctg caggcaaaaa cacggatatg gtcgttgtat
    27601 tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa
    27661 atgataaaat cggctctgtt tccaacaaag cctgagccat cgtcttcccc agactttttt
    27721 atcgataaac aaacaggagc tgagacaaaa atcttgtctc agctcctttt tccgaataaa
    27781 aacggcgttc tcctgccaac tccgcggtaa taagtccagc caaacgtaac atatgaagag
    27841 ctatttttta tttgactctt cttattactt gatgcagaac agctttttta caacctctga
    27901 taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa
    27961 agc
//
LOCUS       DAYYAG010000024        27746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000024 DAYYAG010000000
VERSION     DAYYAG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.024"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1457)
                     /locus_tag="ACHARQ_000914"
     CDS             complement(<1..1457)
                     /locus_tag="ACHARQ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409359.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDRGFQLKDKELKKLYE"
     gene            1579..2163
                     /locus_tag="ACHARQ_000915"
     CDS             1579..2163
                     /locus_tag="ACHARQ_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409360.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2174..2419
                     /locus_tag="ACHARQ_000916"
     CDS             2174..2419
                     /locus_tag="ACHARQ_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409361.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2425..3147
                     /locus_tag="ACHARQ_000917"
     CDS             2425..3147
                     /locus_tag="ACHARQ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409362.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3278..4021
                     /locus_tag="ACHARQ_000918"
     CDS             3278..4021
                     /locus_tag="ACHARQ_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409363.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4027..4392
                     /locus_tag="ACHARQ_000919"
     CDS             4027..4392
                     /locus_tag="ACHARQ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409364.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4408..4938
                     /locus_tag="ACHARQ_000920"
     CDS             4408..4938
                     /locus_tag="ACHARQ_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409365.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5100..5408
                     /locus_tag="ACHARQ_000921"
     CDS             5100..5408
                     /locus_tag="ACHARQ_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409366.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            5539..5823
                     /locus_tag="ACHARQ_000922"
     CDS             5539..5823
                     /locus_tag="ACHARQ_000922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409367.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            5960..6760
                     /locus_tag="ACHARQ_000923"
     CDS             5960..6760
                     /locus_tag="ACHARQ_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409368.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6797..6979
                     /locus_tag="ACHARQ_000924"
     CDS             6797..6979
                     /locus_tag="ACHARQ_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409369.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7090..7407
                     /locus_tag="ACHARQ_000925"
     CDS             7090..7407
                     /locus_tag="ACHARQ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409370.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7410..7553
                     /locus_tag="ACHARQ_000926"
     CDS             7410..7553
                     /locus_tag="ACHARQ_000926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409371.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(7757..8236)
                     /locus_tag="ACHARQ_000927"
     CDS             complement(7757..8236)
                     /locus_tag="ACHARQ_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409372.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8320..9693
                     /locus_tag="ACHARQ_000928"
     CDS             8320..9693
                     /locus_tag="ACHARQ_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8409373.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            9716..10243
                     /locus_tag="ACHARQ_000929"
     CDS             9716..10243
                     /locus_tag="ACHARQ_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409374.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10230..10793
                     /locus_tag="ACHARQ_000930"
     CDS             10230..10793
                     /locus_tag="ACHARQ_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409375.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11215..11952
                     /locus_tag="ACHARQ_000931"
     CDS             11215..11952
                     /locus_tag="ACHARQ_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409376.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11969..12385)
                     /locus_tag="ACHARQ_000932"
     CDS             complement(11969..12385)
                     /locus_tag="ACHARQ_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409377.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            12778..14025
                     /locus_tag="ACHARQ_000933"
     CDS             12778..14025
                     /locus_tag="ACHARQ_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8409378.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14293..16683
                     /locus_tag="ACHARQ_000934"
     CDS             14293..16683
                     /locus_tag="ACHARQ_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8409379.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16732..18567
                     /locus_tag="ACHARQ_000935"
     CDS             16732..18567
                     /locus_tag="ACHARQ_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8409380.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18642..19499
                     /locus_tag="ACHARQ_000936"
     CDS             18642..19499
                     /locus_tag="ACHARQ_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8409381.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            19489..20598
                     /locus_tag="ACHARQ_000937"
     CDS             19489..20598
                     /locus_tag="ACHARQ_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8409382.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            20937..21737
                     /locus_tag="ACHARQ_000938"
     CDS             20937..21737
                     /locus_tag="ACHARQ_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409383.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            21843..22631
                     /locus_tag="ACHARQ_000939"
     CDS             21843..22631
                     /locus_tag="ACHARQ_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409384.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            22863..23732
                     /locus_tag="ACHARQ_000940"
     CDS             22863..23732
                     /locus_tag="ACHARQ_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8409385.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            23752..24099
                     /locus_tag="ACHARQ_000941"
     CDS             23752..24099
                     /locus_tag="ACHARQ_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409386.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            24112..25374
                     /locus_tag="ACHARQ_000942"
     CDS             24112..25374
                     /locus_tag="ACHARQ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409387.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            25454..26830
                     /locus_tag="ACHARQ_000943"
     CDS             25454..26830
                     /locus_tag="ACHARQ_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8409388.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            26827..27456
                     /locus_tag="ACHARQ_000944"
     CDS             26827..27456
                     /locus_tag="ACHARQ_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409389.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            27468..>27746
                     /locus_tag="ACHARQ_000945"
     CDS             27468..>27746
                     /locus_tag="ACHARQ_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409390.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVG"
BASE COUNT        10373 a         3849 c         5460 g         8064 t
ORIGIN      
        1 aattcataca gtttttttaa ttctttatcc tttaattgaa aaccccgatc catagtcact
       61 gcatttggat atagaagccc actataatgt ttacgtaaat tcacttggaa tttctgatta
      121 taaaagtcta acagattaga aataaaaggc ggaaaattac tgtttttgta aaattgatta
      181 tttccattta ggttgtatag taatttgtta aattgctcct gatttaattt attgtaaatc
      241 catggtaata cttccatatc cttatctgct aacctgaatg aactagcatc ggtaagatgt
      301 tcgatcataa aatctatgaa catctctttt tcaccaaaat ataaatattg attatatatt
      361 gtttctaatg ttgaagatgg tagcgaagaa actctcattt tttgctggag tataggatca
      421 actagactat ttaagtgatt ttgtaattct tcattgttgt ttcctagaac tgcaagggaa
      481 cgtaaacggt agtaatattc tatttctgtt agtacttgtt gtttcggttt aaaatcattt
      541 attgatattt gtaaattttg aattgtctcg tctgaaaaat catgttgtaa ttctggatgg
      601 gtagctataa aagtaagtaa aaagaaccct tttgtatacg tgatctgttg attttcacca
      661 agtgatttat taatttcctc tcttgattca gctgtgaacc tatactttgc aatttctttt
      721 tttagaatat ttaaatagaa ttctttatta tcaaaaatta gggcattaag tagtctgaaa
      781 gtactttccc tatctctatc tgcttcctca tttccataag ataattgaaa aacaaactta
      841 aaggctgtct tacataggtt ttctttggct ttttcgctta gtttatttag atagctattt
      901 tttagtgcag agtgaaattc ttcaggtgta acagacatag catgtatgcg acttgcatat
      961 tgtataattt cgatcgttac tttttttcct aatattggat tttttaggaa tataatttca
     1021 taggacttcc gtataacatc ctgacaagtt tcttttgtta tatctcttaa ttcccaagta
     1081 ttaccgttgt aagtgactac aggatgagct gaataatttc tttgatcctt aatatatttt
     1141 aaatcctcat attcatagct atcaattagc tcagttgaac tataagcttt ttctagtagt
     1201 tccatttccc attttggaga gtttggatca ctttttctta gtctagctat atcttctaaa
     1261 attttcttag aagtattatc ctcgtaaata tttgctaaat cagataattt atcaattaaa
     1321 tctaaaataa gcgcactgta attgacagct attgcagaac gataatttcc ctgataaaaa
     1381 ctacttctaa cttcttctaa taatggaata attctaggat ttttaacttt ttttatttca
     1441 tcatctaata taaacatata aatctccttt ttttatttgt atgacaagtt taccataaaa
     1501 aaaaattaaa aagcgagaca tatttatgtt gttgtgttat cataataaca taaatatgta
     1561 gagtgagatg atgaaaaaat ggatttgaaa caatcgtttt acatctggtt atatcctgat
     1621 aatcctatgt caataatggc tgatattttt gtagttagca tcttattgta tttattttat
     1681 ctaattttct ttcacaattt aatcgggtac atgattcttg attggaaagg aatgttcgat
     1741 atagaaagaa aaccatttgc taacaatcaa acctataccg tcttcgaaag tggagattgg
     1801 gaagaaaatg aagttgatgt gatccatgaa attggattag ctttatcagg aatacaagat
     1861 gctgatgttt tggtatcaat gacaacggat actggataca acgcttatga ggtattttat
     1921 cttatgtatt ttatatggcg tgatggtcaa gaaattatca atggtcgtgc tgaaaaaaca
     1981 atcaattcta atactgaaat ttctttttgt atgggtgaag gggaaatgat tgtatataaa
     2041 gatgggtcag caaacattaa aacagccttt ggtgatattg aaacaagtat taaggaactt
     2101 agggaatatg tgcagaaccc atataatttt caatatttag caggaagaat aaatttagtt
     2161 taggagtaaa aatatgaaaa aaattataaa tcaaccaaca aatcacttga taattgaagg
     2221 tgttattttc gtaacaatgt attttactgt aggaaatggc atctcaaata caatcattca
     2281 aaaaaatcca caatacctat tatttagtct ttatgcttgg attgtacaaa tggtttgggt
     2341 ttccatattg atgctgttgt tttattggat agtacgggtt attcttttat tgttaaacaa
     2401 aagaaaaaag gataggtgaa taaaatgaaa tcaactaaat tgaaggttgt attgttatcg
     2461 acagcagcat taataaccct tacagcatgt ggtacaacta atagtgctaa taataacaaa
     2521 gacacaaagt ctagtagtac gatggttact aaaaacagca ctacatcggc atcaacaaca
     2581 atgtcttcaa tgactgaaaa taatgctgaa aagtatcaaa aatacgaaga cattaataaa
     2641 ggtcaaaagc cggatgaagt ttataacaac tttattaata cagtaaaaaa tgatgaaaat
     2701 tatgggaatg atccattgca aaataaaaat ctgactatcg ttctttacaa acctgattgt
     2761 ccagattgtc aaaaagcaga atcaaaaatc atgcaagaaa taaaagaagg atttagttat
     2821 gctccaacat taatgcatga tattaccata aataaaccag cttacgagta ttacgcagtt
     2881 gctgtagata ttactgatgg tgtacctgag tgggtacaaa acattcctgg tctggctgat
     2941 gagaacaatc aatatcatac gccaacagtt gcagtagtaa caccaaagaa agttaacggc
     3001 acactatact gggatgtaat gggaatgaca acaaatacaa ctgatgaaat gattgaaagt
     3061 acaactaaat attatatgga tttgggagca tataaattat atcaacaatc agagcctgtt
     3121 atggacgatc aatccaaagt taaatagatc tttagggcgt aagccctatt tttttaaaat
     3181 taagtgataa caaataaatg atttctgaaa caaacgttct tactcaacta ctcgaaacac
     3241 acaagctaat taactagatt ataaaggaga atgcgtaatg aaagaaatca gtgcaaatga
     3301 agctcatatt ccgcaaaaga atcaggttgg tatatataag gtgcttcatg gtgaaggaat
     3361 ttataaaata cagttactta ccggttacaa taatggtgca aatgggaagt ggttaaaggc
     3421 aacaagtatt cgtgaactta gaagtgaagt agaaaaaatt aggcttggtc tcacaagtga
     3481 agaaatagct aaactgaagg gaaataattc ttttgtaatt gaaaaagaaa aggaaaaaca
     3541 tctactttat cgtgagctga aaattgaaga atactatgaa attgatttac ctgaaaaatt
     3601 aagaattatt gatcaacgtt cagaagaaaa atggaaaact gaaatatatc caagcttaaa
     3661 tgactacaag acatcaggaa agcccatgta taactacaca ggctttttaa cctacacaca
     3721 atacaaatat gcctttcgca aattaaaagg cattaaacaa gctgaaattg ccctgtatga
     3781 gatgaaaaaa gtggatagat tgaaattgat tcaagattta gtgcaaaata catggaatca
     3841 agcgattgtc gagctagaaa aagaaaatgg gaaaatttta actaataaat atctgatcgt
     3901 aaatgaaatt gaagaattaa ttggtttatt ttcatgtgaa aaaggtgaag tcttcttgaa
     3961 gtcatcacca acctattcaa ttttggacag tccgtatcag tttatacagg caaggagatg
     4021 agttagatgt ctagcttgaa agatgactat atccaagcaa aacaagagta tgaaaaaata
     4081 cagatgttga aatctgagaa agtcgaatta tttttaacac aaaaattagc agaggatcat
     4141 attgctaact tagatgctaa aactttagag gaatatgatc ggcagtttaa tcagatacat
     4201 cacaatatca ttttgcttgt tgagcaggca aagcgaaggt ttgaaaaagc ggaatatgat
     4261 ctattagatc aaggaattgt atgcttggat aagggattgc gaagatatta tggtgcagag
     4321 ttaaaaaagc aaaatgtaga tagaaatgca ctaaaacatg aaatcttaga attgataatg
     4381 caacgaattt gaagggatag aaagcaaatg tcacaaaaca ttagtgctgt aaaacaatta
     4441 atagaagaag atttagagac cacagttcag agattagcgg aacgaatcca ttttgcaaat
     4501 tatcaaacca tctctaactg gtcaagcagg aacagaaatc cggaagaaat tccaatgaaa
     4561 tttttttatc gaatcggaaa agaatatggt gttgatccga taaaaatttt cgagaagacc
     4621 atgatttatt actatcatgg aacacctaac gaagtgttag aacatctttt ggaagaggca
     4681 aaagaaaaat catcaatatt agagctatcg caaaatgaca aagttattca agtgaaatgc
     4741 gttgatccaa caggaaagat tattgtcggt tttgatattg aaagaaaatc caaaatagat
     4801 tttattacac aattaaaaaa gataaataat ttagatattc taacgcttag accagatcat
     4861 gaacagttgt ttatgagttt taaaaaacaa ttgaaagaaa tacaaaaaag atataacaag
     4921 gtgtttccta atgcgtgaaa agctcaaaga aatcagagtt ttttggataa ttaacaaaga
     4981 atgtatcatc ttatatactc ttggatttct tagcatgatc ttgttttggt ggttatattt
     5041 aaaacagtaa ttggagaggg tcaagaggat ttttatcaag ataaggatgt ggaaactgca
     5101 tgtggaataa tagattaaaa acaggattgt tattaatagt tatttcctgc gctatgatga
     5161 ttggtatgag gatacaaagg gaacaatcct attttgaagt ttctgctaac aacgtaatag
     5221 aaaaatgcta ttacggtcaa cattattgga gtgaggaagt cagagaaaat atagatcgtg
     5281 aatatgttca aagaattgtt tgggatgcat attctatcaa agattatcct aaaagtttaa
     5341 cgagtagatt attttacagt gaaaaagata atcaaaaact ctcagatttg atgatgaaaa
     5401 aagtatgact attattacga aagagaaaca gagtgctata ttcgagggga aaaagcctac
     5461 aacaaaagtg actattgggg tgacaaggcg aagcgattaa ctgcaaattt tagagtgtat
     5521 gaatcagagt ttataaactt gacctttctt aatagtgttt ttattgatta tgtaattact
     5581 acgaaacgca ttggaagtta ttcaagtacc tacgcatcct tagttccaat gttaaactcg
     5641 atgagccgtt tcttaaaaga aagagaaaag aaagagctac agtatatcaa agaaatcatg
     5701 ccggattttg aaatgcacga atggcaaatt gctttatcag attggaaatt ggcgaataag
     5761 gttcgtagaa taacaccttt tcaagccaag cgctttttga gagctatgaa atttgtgcaa
     5821 taaaaaaagc caccagacaa gttgatggag actaacacaa ttaccaacag ttgtaattgt
     5881 gttagcttac aaattaatta tggtgtttag tcaatcccaa attgtgaaat ggcaccaata
     5941 attggaaagg aaaaatgaaa tgtctgaaga tttacgagag ctgaagttac gaaagaagca
     6001 gtatgatcag tatttttccg gtttgttgaa taaacatatt gaagaaggac ttgatcatga
     6061 ttttggaaaa gatgaatatt gtttatattg cgaacgttac aatgaaaaaa tgtctgaata
     6121 tctacagctg aaaaaagaga tacagaaatt agagaaaaaa gtacatgctt ggaagtataa
     6181 agattccatg tctggttatg ttgaatctca aaataagcca agaaaaaaga agcaagcgta
     6241 tgttctaaag atggtaaatg gggaagagtt aattgtatta gcgtacagtg aagaagaggt
     6301 tcgagaactc gttgaaacac ctgtcaggaa aaaggttgaa acaataaatg agatagacga
     6361 cagccaatac aatagcatct atattgaaac aagtcatggt ggatataaga aattagcaac
     6421 tttccttaga agttctaaag ttgggataat ttctaaaaaa gacataagtg ctgttaccga
     6481 tagtttttgt actttatctg ttcctaaagc ttatttccaa tacgatagta aaagatttga
     6541 acgcttagag aaattctgta gaccgtatac tgtattgaaa actaaaaaat ttaataaaac
     6601 taaaagagtt tctatacagt taccgactgg tcaaatctca ttaggtgaaa aagactatct
     6661 gataaaagta ggaaaagagt taattataaa agtaccggaa cacttattta aagaaaattt
     6721 tgagatacgt gaaaccttgg tatggaaaga agaaaaatag tttggaagac atagaatggt
     6781 ataaggggaa attgaaatgg cagtaaataa aatattgcag gtactaatca tagacgaatt
     6841 tggcggagga gaatggtttg atgtgcagtc catagacttt gaagaagaag tcgtagttat
     6901 agaaaatatt tacggcgaag tatattcata ttcgcttgaa ggtgagaata ttgaggattt
     6961 acggttgatt ggtgagtaga aaaaaacaga aatatcaaaa tggagataaa aattaatagc
     7021 atgtaaagat agtcatgctt gggttaaaaa tgttaaaatt caaaaaaatg agtcagaagg
     7081 gataaaagta tgaaaagaga ggaatacaaa caaagattaa acgaactgct tgaagaggac
     7141 gaaacattaa ctcatggttc gcctgatgaa attctataca tgattgataa tatggtgatc
     7201 tttggtggat atgaacttgg taataggtca gttgatcata atattttaga atttgatgat
     7261 gtatcatggg aagaaattct tgattgggga attttggcgg tacctgaaac taaaacctat
     7321 attagtgaca ctatggttcc tttttttgag gaattggatt ataaaagact tcccaaaaat
     7381 gaaaatcata tactgggagg aaactaacaa tgttaagtga cttatttatt ttactgatct
     7441 atctaggtgt aagtgaaatt attgcgtatt ttttgtttgt cattgctaat tatggtattt
     7501 ccagtgcaat cgttcaaatg tttagattac cagtaagtga actaaaaaaa tgatgagatt
     7561 atcagcatat cttttaatgt ttttgaatgt ggtcacgtta atctttgtag taatcggtgt
     7621 gactttcgat ttaatggaat agtgggaaat ctataatgga aaaaagagac acgaatgaac
     7681 atattttttg aatcaatatt gttgatggtt tttgcgatca gcgcttacca gaaatctaaa
     7741 aaagagtaat gattgtttaa tcatcactct ttttttgtag ggaaattgaa atatgtggag
     7801 cttgtattgg tttagttagg attgcttggc tataaggagc tagttcttta tcaaaaccat
     7861 caaagtaggt tttcagtagc gatttaactg atcgctcatc ttgtttgatt tggataattt
     7921 cagcatccaa atcggttgtg agatcaggag cttttgatgc gattacctga ttttttttat
     7981 cttccaactc tcttaggctg tttaacaagt tgtaaaaaga atcattttga ttgttcattc
     8041 caacgtgagt tgcaatttta acagaagttt cgtccgtatt gtcatcaaaa gaactcattg
     8101 gaatatcaag tagtaacgag gatgtttctt ttactgactt tttctttaca gcattttttg
     8161 tttttccatc ttttttgctc tttactcgag atgtttttgg agctagttta ggtaaatcac
     8221 ttgttttatc agtcatttat tagcttttcc tttctattaa tttatgttat tatattatca
     8281 taataacata aaaaagtaca ctttttgggg gcgtagttat tgaaaaaatc taagagaaag
     8341 attgccacat tgcttatgtt aatggtgcaa tttacaggga ttgcaatgag tgtaatcaca
     8401 ataaatccgc aagtgataca tgctgatggg atagctactt atgatgcaca atttaactgt
     8461 actactgaag gtagtgggtc aggaacagat agcgatggaa acgatactgg aagtggcgct
     8521 gtaacaggtg atgtagccaa aaatattgaa gatatttaca aatacatgaa tggagaatat
     8581 ggcttatcag gtgctagtat tgcaggtatt ttaggtaact ggaaacaaga atcaggtatt
     8641 gatcctcgag cagtagaagg gtattacgga gatggacaga gagaagttga cttcatcaaa
     8701 caatttactg atggtctgat gggtattggc tatggtcaat ggacagctgg gcgacataca
     8761 gcattagttg attggtctaa gaaaaataat ggcggttctt ggtggactac agaagcccaa
     8821 ttggacttta tgttcaaagg tgatggggga ttcgtaggaa tattaaaaga ttatgcttta
     8881 aatgcttctg atgatgtcgt agaaaatgca gtaacttttc ataaaaactg ggaaatttca
     8941 gctgattccg aatcagctgt aatagctgta cgtggtgaaa atgcaaaaga aatctggaag
     9001 tacatgaaaa gcaagggtat ggacggtgca aaagatgaaa gtaagatcaa taaaatatcc
     9061 ggaggttcaa gtaccggaga tggatcgaat aattcatcaa gttcagcatc aggaacagta
     9121 gaagatgcct gtgcaactac ggatgacgct aattctggtt ctggatcaat tgggaaagaa
     9181 ggaaaaatta acggtaaaaa aggtcaagta atagcgaata tgacttatga agaagctttg
     9241 aaaaaatatg ggaaagcgat cacgctacca aaattcaaag aaccagattg gtcaacttca
     9301 ccttttaacg gaatatatca aggtcagtgt acagagttga cgtgggctta tatgagccaa
     9361 ttatacgatg ggaatcaacc gactactgga aatggtggtt atgtatggga aagctacaaa
     9421 tcggcaggag caaaaataac gaacaaacca atcgtaggtt atgggtttag tgcttcggat
     9481 ggatggttat gggctgttgg agatgcaggg catactggtg tagttatagc ggtgtttgaa
     9541 gatggatcat ttctatgtgc taactttaat gttccaccaa cccaagctcc aacaagggga
     9601 gtttcggtaa cgttgattga tggagtggac ggatcagata atgtgcattt ctttagcggt
     9661 gtaggaaaag ctaaatttga aatcgaaaaa taaattggaa tgaaggacga tatagatgaa
     9721 tagtaaagtg aaagatacaa ttatcatttc tttgctttct ggtatgttat tagcactgct
     9781 tgttggaatc ctattcttca ataagcagaa tcaggacata gaagctgaaa aacacagtgt
     9841 gcatattgaa gataagctga cattctttaa agaggttaaa ccaaatgagg tggatggatt
     9901 acggaaagat ttagaaaatg atattcaaga aatgttgaat ggatcagttc aaaataagtt
     9961 tgcagatcaa tcaggtgaag aatttttgag aaaactattt attgtaacag gtaaaccacc
    10021 aattactgat aagagtagtg agaaagaaaa aatcgagtac tataagcctt ttacttttaa
    10081 gctaacaaac tttggaatta agacatctag tagtgggttg tatgaagcat tgaccgattt
    10141 tgaagttcaa tacaatggaa aaaaagtgta tgataccaca ttaaccatca cactaaataa
    10201 tgataagaaa gtgactgggg gtgcttttta tgcaaaatca tgaaatcact actgctaaaa
    10261 aggcgatcgg aatactttcc ataattgtga tcctttgttg tgtggtacta tttcaaaaga
    10321 aaaatgctta tgataataat gtcaaagaat tggaagacct taaaagtaaa caaatcgaag
    10381 tagtcaagaa taaccagaaa gtgaaagcta tgcaggaagc tcaatacaaa gaattgggaa
    10441 atgctgaaat taaggaacaa agtaggaaat tctttaattt attttacaac tggaattcat
    10501 gggaaaaata tacgaagaat atgaaggaaa tacaaaaaga atttcctcag atagtaaagt
    10561 ctagtcctgt tgatatttct ggcacgatgg taggcactgg taatagtcca atatctagct
    10621 atactgctga ttactataca acaaatcaaa aaggtaaaat ggttgaattg attacacaga
    10681 caagaaatac tccaacaaca gcaacaacaa caatttggcg tggagtaaca agcataggag
    10741 aaaatcaatt gttttatttg gaggatttag atagctatgt aagagcagag taaaataaaa
    10801 aaagagctaa cggtaccaac gttagctctt tgtgctgacg atgataacaa aacctaccga
    10861 aaaaggattg tttcatcaca aacacaaaca gaaaagaatt gataaaaaac ccgaacctca
    10921 gaatttttat taatcaattc atttagatta taataaattg agctgaacat tacaatacgt
    10981 atatgttgta aaacgtaaaa aaagtcaaaa gtaaatactt tcttaaaaag tcaaataaaa
    11041 ctattaaaga caatatatat ggtttatttg acataagaaa atggtctaga tgataattgt
    11101 aatatagaat tgtagactta aataaagcat aaaatacctt ttatgataag gtatagctct
    11161 taattttaag cagttttaga tataagattg aaagtaaagt gggggagaat aataatggca
    11221 gatcataaat tagaaaagtt attagaagaa acgtggaatc caaaagaatt tagtgaattt
    11281 tttatggaga attttgaaac tgacttagca gtgattgtta aagatgctct tagagagcaa
    11341 gggtatcctg aaactgcaaa ctatataaac attaatttta ctttgtatac tgaaaataaa
    11401 ggaacttggg atttttgggc tactttagca aataaggagt taagtgataa atcagacaca
    11461 ggaattcgta acttttttga atcaaacaga gatgattata tgtatgctaa caatcaaaat
    11521 aaattgaact ttagagtaga gtttgatgaa acacctgaag aatttataga aagacaaccg
    11581 cctaaagaaa atgtagcaaa ggtattggaa gatcgttgga attcagatga aatagtttca
    11641 acaattagcg aactaggcgg acaatatgaa ccgctagtag aagcggttag agaagagcta
    11701 agactgaata agtttcctga tgttcaaaat attgatgttt ctcaaattga aataaacgtc
    11761 aagatcacga ataaacttga ttatggtagt tgggcagata tagcattaga aaaatacatc
    11821 tactcaacgt taaaagagtt tatcgaaaat agaatggata tcatgtatct acagcatcca
    11881 cagtatttaa actttggtgt tgaaatagct acacctcttg aagaatggaa aatggagcaa
    11941 gggcttgact agcctttgct ccattttttt attttacaaa tgaatacagt tcgtcgtttt
    12001 taccaagaat gattgtttgc ccaactttga gattgtcagg tgatccttca gcggtttgta
    12061 aggagaacgt tccattgaac ttatcggata ccgttatagg tagctttaat tctgatcctt
    12121 cctcatcttt gagaattgct gtttttcgtt catcaaaata aacagtcttt attaaataat
    12181 ttttcgtagt gatctcagca gatttgtctt tttttgtagt agtaccagtt tctttagaat
    12241 tagaggtatt ttctacgact attccatctt tggcatttga gcttgtgttt atttgattga
    12301 atattttcag cgacaagata acgctaagaa tagtcaatat agaaacaagt gtaattaata
    12361 ctgataattt cctttttttg ttcatttttt cttaacatcc ttttttgttt tgattagtta
    12421 gatattcctt atctaacaag cctttttagg ctattaaatg aagataaacc ccaaaaaaat
    12481 attgcaatta tgacagtgca atggtactat tattatacaa taaattttgc gaaaggggta
    12541 ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa tacaacttgt ataccgcaag
    12601 ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt acaaacataa tcaactatta
    12661 tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt gacaaaacct atattataac
    12721 ggaagaagga atagaagcat tgcgatcaca aacagaaaat aaaaacaaaa agaaggaatg
    12781 aatatgaaga gaaattcgct tggtatggtt ttattggcat cagctgtact tgcggggtta
    12841 tcagtaccag taacctcaca tgctgatgaa tggactgcaa acacaccgga tcaaatccaa
    12901 attacaacag gacaatcaag ttatgcgttc aaaaaaggag atactatttg gtcaactgta
    12961 gagaagctta aaagcttagg ttacaagact actgttgctg agtttgccga attgaacaat
    13021 gttgatttgg caaaaggaga acaacggact atcccgattg gacgaacttt caacatcagt
    13081 aaagatacat taacagttaa ggaagcagat ggcgaagtag ttagtcaaac tgttcaaaaa
    13141 gataaacctg atcctattga aaatacggag caagagaaca aaaaatccga gactacaaaa
    13201 gaatcggaac caacagcaaa cacttctgat aagacagaaa caagtgcttc aagtgttgat
    13261 ggagcaagtt catctaatac aaataattct acaagtgaga aagaagcatc acagggaaca
    13321 agtgaaagta gcactacgga agctgatact ggaacatcaa caaataactc acaatcaaca
    13381 tcaaatgatg caaatgaatc aaccagtaca tcttctagtg atcaatcaca aacaggttcg
    13441 agtgatgaaa ctgacagttc tacttcacca agtaaacccg aacaaccaac taatgttgtg
    13501 ctaggcgttc catttattag tcaaggtaac acaatgcttt gcgaaggaac atctttgcta
    13561 gaagcgttac attataaagg tgttgcaaca aatcaagatt taatgacttt tgttaaatca
    13621 atgccactat ctcctgacaa taatccaaat aatggttttt ctggtgaatg gagacacaat
    13681 gtggatggaa cttatcaagg tatgaatccg gctcctgtag tttcatgggg tgttgctaat
    13741 ggcggaaatg ttgtagatat ttctggttca tcggtacaag gattaaaaga tgagattgta
    13801 aatggaaatc ctgtagtagc gtgggtaact tatgcctttg aacctgctca acataaacca
    13861 atgttttggg gcgatgctat ttggaatcga catgtagtag ccgttgatgg atttaaagac
    13921 ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt ggattaatgc aggatcattt
    13981 gaaacagcat ataacgtatc tcaattagcg gtttcggtac gttaataaaa gatatcgttt
    14041 atatactaag tagttatata gtctttattt gtccttgctt aactacttag aaaacttgtt
    14101 gctcatggtg ggtagaacta aaaggggtga tgctatgaat actagagcgt tgaaacagct
    14161 tgctttcaac gaaaaaaatg attaataggt gtggtgattt taacttagga atgggcgcga
    14221 aggtcattac cacaccgaca tatcgtggct cattttcttc gcctaactgg tataaagaag
    14281 gagtaacgag agatgaagca tgaaaaaacg ataattttaa aatcaaaaat aatttattgt
    14341 tttgcgactt tagtaatgat gttatctacg cttataatgc ctttttcagc aataagagct
    14401 gaagcagatg tcacaaaacc gacaagtgca aaatttatag aaactggcgt aaaaacagac
    14461 ggttatatta gggttaatat gccgaatcat ccaaatgaat ggatgatttc aagtcaattt
    14521 aaagattctc atggaaatat tggatattgt atggattcag aattgccatc cccaacagga
    14581 agtggagccg gaagcttgaa gtacaaggga gctggcagtg atgagttcta tcgtatgttt
    14641 aaaggtggtt ttccatcaaa aacagcaaag gaactgggtg caggaaatga tacagaagca
    14701 tggtatgcaa cacaattagt ttcatgggtt ttagccggaa actttaaagt ttcacagatt
    14761 gtatggtcac atccaaacca taccgccgca gaaacagctc gtgtaaaaaa agcatttgaa
    14821 aaaatttatg actatgctaa gaatggaaaa gatacgccca acactgagtt ttctatcaca
    14881 gcttctaaga cagctgacga aggaaaatat cacactttca catataaaac agcatcaaat
    14941 aagactggta atgcgaagct cacttttaca tcagcaaaac cagctggtat gaagatttat
    15001 gatgcagatg gtaaagaaat tactaacaac actgtgaaat taaattcaag ttttactatt
    15061 aaagtacctg tgactacacc aagtggtact ttgtcattta aaggtactgc taatgttagc
    15121 acaaccaatc cttttacttt tgatggacga ggtgtgtatc aagatgccgt agttatgatc
    15181 actacttcag aaacaaagga ctctaaatct ttgagtgcta aatggacaag agctgttggt
    15241 caggtaacag ttaagaaagt tgatcaagat ggaaatgctt taaaaggagc ggagtttact
    15301 ctaactgaca ataacaatgc taaatctgtt aagacaaccg gagatgatgg gttagctacg
    15361 tttgatttgc aacaaggctt aacatacagc ttagaagaaa caaaagttcc tgaaggttat
    15421 caaggttcat ttaaaaagga tggtattact gttaataccg atggacaaga atttagttac
    15481 actgctacga acacaattaa aaaaggtaaa gtaaaagtta aaaaggttga ccaagatgga
    15541 aaaccattag ctaatgctga atttaccttg acggatgatt catcccaaac acaagcagaa
    15601 aaaacagatg aaaacggact tgcaagcttc gatattgtag caaacaagac ctacagcctt
    15661 gaagagaccg gagtaccaac tggttataca ggcgtattca agcaagaagg aattaccgtt
    15721 aaagatgatg gacaaacttt tgaatataca gctacaaaca ctttgaaaaa aggtttagta
    15781 aaagttcata aagtcgatca agatggaaaa cctttggcaa atgcagagtt cactttaacc
    15841 gatgaaaaag gtgcaacaca agtagaaaaa acagatgaaa atggacttgc aagcttcaat
    15901 attgtagcaa acaagacgta tagcctagaa gaaaccggag taccagtagg ttacacaggt
    15961 gcatttaagc aagagggtat caccgttaaa gacgatgggc aaacttttga atatacagct
    16021 acaaataatt tgaaaaaagg tgtagtaaaa gttcataaag tcgatcaaga tggaaaacct
    16081 ttagcagatg cagaattcac cttaacggat gaaaaaggtg taaaagaagt taaaaagacg
    16141 gatgcaactg gtctggctac gttcaacatt attgcgaaca aaacttacaa cttagaagaa
    16201 acaaaagtac caacaggtta cacaggcgca ttcaaacaag aaggaattac cgtcaaagat
    16261 gatggacaag tctttgaata tacagctaaa aacactttga aaaaaggtgt gatcaaagtt
    16321 cataaagtcg atgatcaagg gaaagcatta gcaaacgcag aattcacttt gacggacgac
    16381 aaaggtgtaa aagttgtaca aaaaacggac agaaatggct tggcaacatt taatattgtt
    16441 gccaacaaaa cgtacaaaat cgaagaaaca caaaacccta atggttatac agggaaatgg
    16501 tctaaagaag gcatcaccgt tgctgacgat ggacaaacct ttgaatatac agtaattaat
    16561 caccttgtac ctgaaacagt aagaacaggc ggaatcggta catcagcctt ccttcttgta
    16621 tttctaattg caggattgag tatggtgcta ctgtattatc gagaacagca caaagcaaaa
    16681 taaaaaagtg ttaatggaaa agaagtaaat atgaaaaaag aaggctgacg tatgaaatca
    16741 aaaaaaattt tttcaaattt aatgctattt ggtactttgc taggagtggg attgggcgca
    16801 agctcaatct cttctagcct tggactaggg gggcaagtag catatgcaca acaaaactat
    16861 aattatcaag gaactggaac gattcatatc cataaagtaa caaatactaa tggggatggg
    16921 aaccctgtct cagatattga taacaaaggg aaagaaatag ctttaccaaa tggtgtaaaa
    16981 cctcttgaag gcgttgagtt ctcttatgtg aaacttacta aagatgaagc taaatccttg
    17041 ggatggacgg atagtgatac tacaaaaggt caacatattg atgatgctaa agcacgatta
    17101 ttactaaaca aaaaatacag tgaagtaagt agaatgaaga ctgattcaca agggaatata
    17161 gacataccta tacaagtaaa tacagacgat ttaacagata acttgtattt gattgttgaa
    17221 acaggaacag tcaaaggtgt agttgaagaa gcgtatccta tgattgttaa cgtacctgaa
    17281 ctaaacggat cagatgatca atactttaat tatgatgtac atgtctatcc taaaaactat
    17341 ttccaattaa actcagcaga gcttgataaa aaagacgaag atggaaaggc tcttgccgga
    17401 actgtttttg aattgcgaaa agatgatgga tctgatcagg ttgttggaac ttataaaaca
    17461 gacagcacag gtaaattaac tgtggatggt ttggaattag gtgactatta tttcctagaa
    17521 accgatttgg gaacagatgc taatagaaca acctatttac tagatggtaa aaaattcaaa
    17581 tttagcgtta aacgtgggga gactgctaaa gtagttcttg atgttaccaa ctacaaacga
    17641 cctgtaatta ctaaggtaat tgataaagaa accgttgata ctggcgaaga tgttcaatat
    17701 acattgaaac catcaattcc tggtaatatt ggtaattacg aaaaatatgt tgtcaaagat
    17761 gttctaggtg cagagttaac ttatgattca tcagaagtga tcgaaggaat gactttagat
    17821 actgattaca gtatcgaacg tgacggacaa acgacaaact atgtctttac tgaatcaggt
    17881 atggataagc ttcagaaaca atttgatgca ggggatcgct tctttacaat tttgatgaac
    17941 gctcatacta atagtaaagc taaaatggat acaccactta agaatgatgt ttcaacgatc
    18001 tacaacaatg gatatgaacc cgaagacgaa acaccagttg atcacgctta tacaatcatt
    18061 ggtgatcacc aattcattaa aacagacgga gatacaagca aagcattgag tggagcaaaa
    18121 tttgtagtga agaacaaaat tgatccgaat aaagttactt acctaaaaca agatccaaca
    18181 acgaaagctg ttacttggat tgcagataaa tctaaagcta ctgaattcgt ttccggatca
    18241 gacggtaagt tccaaattga aggtttgcaa tatacctatg atgtagattt tgataaacaa
    18301 ggtaatccaa caatcacaga taaaaaaatc aatgattatg ctcttgaaga agttgttgct
    18361 ccttcaggat atacaccaac tagaaacgat gttccatttg tggtatcgaa tgatgatgct
    18421 tctagcgaag taacagacat caagaacctt aaaacacctg aaacagttcg tacaggtgga
    18481 attggaacag tagtatttct agtggttgca ggtgtggcag gaacagggct atatgttgtc
    18541 tacaaacgag aacaacacaa agcttaatga aataaaattc cctagagtgt ttttggctct
    18601 agggtttctt tttaaaaagt gtgtgaaaag taggtatgag catgaaaaag aagaatatca
    18661 agttgatcat cttaaaaatt gggatgttgc tatgtttagg agtaggtcta tatcctgtaa
    18721 ttactcaggc ttatacctac taccaaactc aacaaacact aaactcctat aaggaaaaat
    18781 atgatgaagc ttctgaaaag gagaatgaag aagtcttcaa gagatattca gaagttaata
    18841 ggaaacgaaa agacacagaa caaacctcag tcacgattcc tgaagataat aacaatattc
    18901 cgtcaaatgg aaatcttcag aaggataaat taggtgaatt agtgggtgaa attcttatac
    18961 caaagattaa tgtggatttg ccaatttatg atggtgtaaa cgatttccaa ctatcagcag
    19021 gagcaggaat attaaccgga actgattatc caactggtgg gattggaaac cattctgtta
    19081 tatcagctca ttcaggcatt cctaataaaa aatattttac agatttagag ttgctcaaac
    19141 ttggtgataa attttttatt gatatttcag ggaaaaaact tgcttatcaa gtagatcgga
    19201 taaaaacaat tctaccaacc gaatttgagg atttgcttcc tgtggcagga aaagattatg
    19261 taacacttat gacatgtaca cctgttggag ataattctca taggttactc gttagagggg
    19321 ttagcatacc ttaccaaaaa agtattgttg agaaagaaaa gaagaaagaa gatttaaagg
    19381 taaaggcgca gatgaactgg tatcctatcg tagttgtagg ggttttatta ataatagctg
    19441 tgatagtcat tattaaaaaa atagtagcaa agagacgagg aatgaaaaat gaagaataaa
    19501 atactacttt ttttgactct tatttgcact ttggttttaa caatttatcc tgcaaatcag
    19561 ctctatcatc aattcaaaac aaaagagaat gtggcggaaa tccaagagaa aattgagaaa
    19621 aaacaaactc cttcaaaaga ggaagtggca aaaaaagtcg aacaaataaa atcgaacagc
    19681 ggtaaccaag gattagacaa tactcagaaa acagataaag gagtagacaa tactcaggaa
    19741 acagacggtg gatcatttga tattcgtgaa gctgaaggaa aggtcaatcc atacacagga
    19801 gatgtttttt caaaaaatca aacgaaagat tttgaaccat ctacctattc aggagcggtt
    19861 ggttatgtct atattccaaa aataaatgtg ctagagcttt tgtttctagg cgcttcacag
    19921 gagcatttat atagaggtgt ggcacaaatg agtgctacta gtctaccttt atcaggtaaa
    19981 ggtcatcaaa cagtaatagc aggtcatcgt ggcggttata cgtcaccaca atttctttat
    20041 gttgatcaat tagtagccgg agaccacatt tatatagatt atcttggtga aaaagcagtt
    20101 tatacggttt tccgaacaga cgttattaga aattacggtc agaattcaac actgactgat
    20161 attcctgatt tagatatgtt aacactttat acttgtcatc catatcctca gaactacgat
    20221 cgtttattag ttagaagtct aagagatgaa aaagcaaaat acatcacgcc acaagtaaca
    20281 gttggcaata ccgcatacgc tgatgccatt gatagcgagt ccgtttcaga taagacagaa
    20341 actgttacat cttctaatga tttatttgag gatgttgatc gtgccaacaa ggaattagat
    20401 ggaaataatg atcttaatat tccggaacaa ggtagcgtag agatgcaaga cagaacgttt
    20461 gctgaaaagg caagttcaat atttacagga attaccaata aacatcagaa aatgctttta
    20521 gtcatttgga ctgtaatagg agcattagca agtctaaagg ttcttattct tttaatcaaa
    20581 gagtttcgaa aagcataatg aagattataa aaattgatag agtacagtaa aaagggattg
    20641 atactattat tgatctcttt tttattttga atagagctag attcactttt aaataaaatc
    20701 aaaactgatc ttaattctaa agaaaaagtc aagaaaaaaa tacaacaaat ttttttatca
    20761 gaatatttat cagaatatta ttagaaaaga taaactcaaa agcttaattt aatagggtta
    20821 ttggttgtat ttagaaataa tgtatggaaa agctataaat caatagataa gatgtagaaa
    20881 atcttatttg tgaatgaaaa acaagtcatc gtaaaatatt aataggaggt gtagtaatgg
    20941 tgggaatatc aaaaacagct ttagactcat tagctaaact atttagtagc aggtttgatg
    21001 gtgcagaatt tgttgaatat ctaagccaaa acgaaagtgg aaaaactctg atcgaaacaa
    21061 tgaaaaaaga ctatttgaat gaaaatgact ttccgacatg gacgaatatt gaagatgcta
    21121 catgggatgt agtatgtacg atatattctc cttgggtaac gattgctgat tttgttttag
    21181 ataaaattgg aggggataca taccctgatg ccaaatctct tgctacaagt gactttaaaa
    21241 cgttctatgg ttatctaata ggtctaatag gcaatggaat agatttgaaa gttacgccaa
    21301 aacaaacacc tgaagaaatt gaaaaagaaa aaggagaggt tcaaatggct tatgcaaatg
    21361 acagagaaaa agtaaaatta ttggaaggta aattatctga agaagaactt ggaaaaatgg
    21421 tgcaagaaat caatgaagac agcaactatt ttcctgaatt gaaggtatat caaaatgacg
    21481 aagatttctt taatgaaatg tttagtaata gtccatggga agcagtgagt aaaacaaatt
    21541 taggagaatg gaactttcaa gatgatctag ttgcatttag caatgatggg tatttacgat
    21601 catattcaga aagtgagtat agagctgaat taaaaacgta tgaaacggat attctgatga
    21661 gctatttaaa tctattagag agtggtcagg gagagttaag agataaagtt gaagaactat
    21721 taaaagaaga gctataggga ttatttcaac ataaacacaa gcagaaaact actaaacaaa
    21781 acgtaccatg caaaaagtcg gttaaaccgg ctttttgctc tattatgaga ggagatcaaa
    21841 ccatgagtgc aacaaccgat atggctttag aagcagtaat aaaagataat acaggaaatg
    21901 tcgaaaacgt agaagacttt agcgatattg caatcaatct ttcagcatgg atttcagctg
    21961 taggagaaga aaatatcgaa tttgagatca agaatatgga tgacttaaaa gcaaaatttc
    22021 cttttatcgc atcgaaacta agtcaagttg ccgaggataa atcgtggaat gaattagtta
    22081 gtttttccga aaaaatttat gatattacgc aagaagatga gtgtgtttca tgggaaatga
    22141 ataatcaata caagaaaatt gacgctttcg tcaaatcacg tatagaagga acagcttttg
    22201 agtttgaagg tatagatacc gcagataatt ggttaaataa agaatccttt attaaaagta
    22261 tagcggatga tcttgaaaaa caatggtcta aggaagacta tgcagagtac ctaaaaacag
    22321 aaagagatac agaggtgaaa gaaaaactca ctgaggtatg tttggatgaa catattgatc
    22381 ccaatatcgt aaaacaagaa gactatgaag tggttattga taaagttcct actgattttg
    22441 aggaagttgt aaagtatcta gttgaagaag cattatcgca atactcttca gatgaggaag
    22501 tggacgaaga aactgttcgt accagagttt ggcaactggt ttttgatgac aaagattata
    22561 aatatttgag tgatcaaaac tcggttaatt ggagtgtacg attattaatt gatcaaaatg
    22621 atattgaata aaatagagct gaaaagctct attttttttg ttaaaaaaac tagctatttt
    22681 attaaaaata atttggttca aattttattt ttttaacttg ataatttctt gccatccttt
    22741 tttaaattgg ctatatatca aggtttatag tgggaataaa taaaaataca tttgacaaaa
    22801 tttacaatat agtttattat atatttatct tattatgata acacaataaa aggaatggtg
    22861 gtatgaaaaa gaagaagttt agtcgatcgt tgttaggaat tttggtttta gttgcggttg
    22921 gtcttagtgg caagggttac tttcagaata acagcactca ggaacatgat tttcagatca
    22981 aaagtagtgt tcagataggt aataaagcag acagtgaatc aaaagaattt ttagggatac
    23041 gttatgatcc aaacaaatat ccaaataata tcgtaaacat taatgatggg aaatatacac
    23101 tgacaaaaga agaccatgaa gcattatcag aaaaaggcag tcaaaccttt tgggtaaatt
    23161 atgcagattt agatcaactt ggaagagcag gagatgttga agcgttgatc acctatgatg
    23221 ctgtaaattc tcactcatct aaaaatgtgg aacgtccatc tttcaaaagt tctgtccata
    23281 ttgcaggaga atacaaggat ggagtatttg atcaagagag agatacttgg gtcggcaaag
    23341 attcaaacaa tgaaatcact cagctagaag gctataaagg ttatctctat aacaaaagtc
    23401 attcattagc atggtcgcta ggaggagata tggaggtaca caatttaaca cttggaacga
    23461 gagcgcaaaa tgttgggaaa aatgatcaga atggagggat ggcttactca gaaacgattg
    23521 tacgtgatgc aatctataaa aataagaatg aaaaagtatt gtataaagtt gatccaattt
    23581 ataaagggaa tgaattagtt cctcgtggtt ctcatgtcga agctttctca gttaacgata
    23641 atggaaaaac ggtaaacctt aatgtttatg tatttaacaa tcaaaaagga atcaacatca
    23701 actatcaaaa tggtacttgg gtaagagatt agaaattgag aaggtgaaga attggaaaca
    23761 ataagcatca atggattaga taaatcagtt gcaaatcgaa taaaaaaagg aaatggctac
    23821 tatcttctga agaagggtaa atattcagtt gctagagagt tagaaacaat gagtatcgtg
    23881 tatgtagcta agaatcattt tgacgtagaa caggctattt ttgatccaga ttctgaaata
    23941 gggaaatgtg ttgaattatc agagaaaact gttagtacaa aaggggcgat ggtcattgag
    24001 agagaaatgt tagacgaaat cagagtaatc attcctaaag atacgcagat gtgtgaggac
    24061 agcataagtg acgatgaacc aaaatcagaa agaaaataag gagatattca gatgagcgaa
    24121 aaatccattg aattaaagga atataagcga agcgatggag aaaaatatta tcctgtagcc
    24181 agtttttcta aatcagtata tctggttgga cgtgcattgg tagttggtgc aattggcata
    24241 tatattttgc attgggtaac aaaggttgta caactgcttg tagatacctt agtaagtatt
    24301 cttgtctcgt taaatgatgt taacggaaca gatgatttag gattaaaaga gatgtttaaa
    24361 gagttactca atgcgttttt tcccaattat ttaagttatt catataatgt acaaagcttt
    24421 tttgcaaagc tacacttggt agacatctct attttagtat tagaagcagg aacggtattc
    24481 tttgttggat atttggttac tttgatgttc aggcatcact atggagaaca aagaccattt
    24541 tttgatgata tggaaagtag aaggttgaaa agagaagctc tagaagctat ggatgtgaag
    24601 tatgatccaa acaagattat aggagatgaa acgaacaaca aaaaaagaaa acgaaacaaa
    24661 acgcaattag aatccgatgt gctatcaacg cttaaaaata tcaaaatttc tgttcataca
    24721 agacgtgatc taagtgtcaa tgaagatatt cgagaatata tcgtaaaaat cccgcaacca
    24781 aaagggaaga aagttagaga aacagtttta cgacaaattc aagatttagg gtcgatcctg
    24841 acaagagcaa caagcggacg agccaaattt ggtgaaaaag aagatgaagt ttcaggcgat
    24901 tatttcattt tccgagcatc agtaaatatt gaaagagaac gtaaggaaag agtaagtacc
    24961 aaaaacaaag aaacggaaag agcaacatct tcaatcgcag tttcaaattc acaattttct
    25021 tttccaatca atctctttgc cgataattcc aaaaatatcc aaaaccaaac cgaaaaagct
    25081 agaaaatatg ccgaaactca aaagcaatct ttagaacttt atctatcttc tgctgacgta
    25141 cctgcaagtc taacagatat gcacgttggg aatacatcta ttgaatattt gtttacttta
    25201 tctcgtacca gaaaaattca actaagctca atgaaagaag aaattgaaac ttacttgcat
    25261 atatcaggtg taatcgtgcg gatcaacgca gggcgtattc aaattacttt accattagga
    25321 gaaaatgatc gtattccaat tgatgtacca tcaatgatca atagcgtgtt ttagaagaca
    25381 tcacaaatag aaacagaaaa tacaaactac cagttcacaa acagaaaagt accgagaaaa
    25441 gtgaggaata gaaatggcta tgaaattatt agagaaagtc aaaaagagag ccgaaaaagc
    25501 tgaatctatt ttgctagata aagaacctaa tataaagctt aatggaaaat tcagtgtcaa
    25561 tatgttgaac acaaaggcta aagacccaac agatgctatt tttggaatta agcctgatgg
    25621 cacaattgct catttttgca tcagcgaaat gccacacatg ttggttgcag gaacgacagg
    25681 atcagggaaa tcagtattac tgaatgaaat tcttgttaca gtaatgtgtc actcaacgcc
    25741 tgatgaatta aaaataggaa ttgtagaccc aaaaagggtt gaatttggaa gatacaaaaa
    25801 attgccgttc atgttggctg atccaattac agatatggat gaagcttatg atttttttga
    25861 atatcttgtg atcttgatgc atgaacgtta caaactgatg gaaaaagcag ggttacaaaa
    25921 catcacgcta tataacgaat atgccgaaaa aaatggatta gatagatttc cctatgtgat
    25981 cttattagtt gatgaatatt cacagttggt tggaacacat aaagaagtag aaggtttgat
    26041 tgttcaatta ggacaaatgg caagggcagc aggtattcat gttatcttag caacacaatc
    26101 tcctcgatca acagttgtca ctggtattat caaagccaat tttccgtcac gtgtttgcct
    26161 tatggtagca agtgatttag aaagtaggat tgtattagat gaaggcggtg gagaaagtct
    26221 aagtccaaag ggagatatgc ttattaagct tgttgatgga agtatggtta gagcgcaggg
    26281 agcttacatc agtaacaaag aaattgaaac aatcttcaat caccttagaa atactatgcc
    26341 agaaccagaa tatgttgatt accgagcaat cgtagctgaa actcgtggtg agggcggtga
    26401 agacgaggat tttggtggaa ttggtaaagc tcctgtaaga aaatctgcta aaagaaattt
    26461 gaacacacct ttagcaaatc catcatctaa agagccatca attgaaaaga agagtgcgtt
    26521 tgctattaag cttgaaaaaa ataaagaaga aaaagctaat cagggagcta agtcactaga
    26581 aaaacttcag caagaaaaat tagaaaaact agaaaaagca ttatcatcat caacttctga
    26641 aactcaatct tcaaagggta tagataatgg acaatcaatt acaactaaaa acaagcctaa
    26701 aaatagtaat tcaaatctct cggtaaatgt tgttatcaca actgataaat ctgaaaaaaa
    26761 acaagtgaac ggaactactt cacccatcat gcaaaaaatg ataaaggcaa aaaaacaggt
    26821 gcagaaatga ttatcattag ttcctttttg agatggctaa caccatataa atatacagat
    26881 gaaatagttg agaagcgagc gaagaaaaaa gttaaaaaaa cgatgttaac tcctaaagaa
    26941 atgctagatc gtggaatatt gggagtgaag gttttagaag taaaccaacc gtttgaattg
    27001 gataatgcga gagataggtt gcttagagct gaagttgatg tcgcatactt ttatcatgat
    27061 aaaagcaagc atcaaatagc tatcaaattc aatccaaaat cggcatggcg ataccaagca
    27121 agagatgacc taaagcgtag gaagctgaaa ccgatgctct atcctgaagg aaaagaggca
    27181 tttgatagaa cccatattgt tcccattgga tttcatggta cagagtcaga taaccgccta
    27241 ttggtagggt ggaacagctc acaaaataga gttcagatca ataaatttga gaagaaggta
    27301 agaaaattta acgatcggaa aataatactt tggtttgcca atattcagaa gcaaccagat
    27361 gggacagcaa agtgggaagc ggtcatattt gatgaagatg gcaaaaaagt aatgtctgaa
    27421 gtatggcatg ataaagctaa atttgtttgg cagtagaggt gaaaattgtg ttaactaacc
    27481 taaaactata tagagaggga tttaacgaaa aagtaagtat cggtgaatct attgaatatt
    27541 taggaaagaa gtacattatt attcgtattc ttgaaattaa aaaactttat tttagtaaat
    27601 ataataattt agcagttaga gttctcgtcc aaaaggttgg aaatccaagt gactatagaa
    27661 gtgattacca taactataaa acagaatcat ctgtaattaa gcgttataat caatcaaaaa
    27721 atgatgagag aatattcaaa gtagga
//
LOCUS       DAYYAG010000025        27745 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000025 DAYYAG010000000
VERSION     DAYYAG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.025"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..1455
                     /locus_tag="ACHARQ_000946"
     CDS             286..1455
                     /locus_tag="ACHARQ_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8409391.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1928..2482
                     /locus_tag="ACHARQ_000947"
     CDS             1928..2482
                     /locus_tag="ACHARQ_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8409392.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2655..3092)
                     /locus_tag="ACHARQ_000948"
     CDS             complement(2655..3092)
                     /locus_tag="ACHARQ_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8409393.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3140..4087
                     /locus_tag="ACHARQ_000949"
                     /pseudo
     CDS             3140..4087
                     /locus_tag="ACHARQ_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4358..4793
                     /locus_tag="ACHARQ_000950"
                     /pseudo
     CDS             4358..4793
                     /locus_tag="ACHARQ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4809..5642
                     /locus_tag="ACHARQ_000951"
     CDS             4809..5642
                     /locus_tag="ACHARQ_000951"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8409394.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5887..6912
                     /locus_tag="ACHARQ_000952"
     CDS             5887..6912
                     /locus_tag="ACHARQ_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8409395.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7050..7175
                     /locus_tag="ACHARQ_000953"
     CDS             7050..7175
                     /locus_tag="ACHARQ_000953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409396.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7200..8315
                     /locus_tag="ACHARQ_000954"
                     /pseudo
     CDS             7200..8315
                     /locus_tag="ACHARQ_000954"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8398..9294
                     /locus_tag="ACHARQ_000955"
                     /pseudo
     CDS             8398..9294
                     /locus_tag="ACHARQ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9310..10134
                     /locus_tag="ACHARQ_000956"
     CDS             9310..10134
                     /locus_tag="ACHARQ_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409397.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10166..11476
                     /locus_tag="ACHARQ_000957"
     CDS             10166..11476
                     /locus_tag="ACHARQ_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8409398.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11624..12883
                     /locus_tag="ACHARQ_000958"
     CDS             11624..12883
                     /locus_tag="ACHARQ_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8409399.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12893..13765
                     /locus_tag="ACHARQ_000959"
     CDS             12893..13765
                     /locus_tag="ACHARQ_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409400.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13777..14607
                     /locus_tag="ACHARQ_000960"
     CDS             13777..14607
                     /locus_tag="ACHARQ_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409401.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14647..16830
                     /locus_tag="ACHARQ_000961"
     CDS             14647..16830
                     /locus_tag="ACHARQ_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8409402.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16891..18111
                     /locus_tag="ACHARQ_000962"
     CDS             16891..18111
                     /locus_tag="ACHARQ_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8409403.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18108..19568
                     /gene="gtfA"
                     /locus_tag="ACHARQ_000963"
     CDS             18108..19568
                     /gene="gtfA"
                     /locus_tag="ACHARQ_000963"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8409404.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19645..20751
                     /locus_tag="ACHARQ_000964"
                     /pseudo
     CDS             19645..20751
                     /locus_tag="ACHARQ_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21073..21282
                     /locus_tag="ACHARQ_000965"
     CDS             21073..21282
                     /locus_tag="ACHARQ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8409405.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21275..21424
                     /locus_tag="ACHARQ_000966"
     CDS             21275..21424
                     /locus_tag="ACHARQ_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8409406.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21578..22243
                     /locus_tag="ACHARQ_000967"
     CDS             21578..22243
                     /locus_tag="ACHARQ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8409407.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMSRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22527..23612)
                     /gene="xerS"
                     /locus_tag="ACHARQ_000968"
     CDS             complement(22527..23612)
                     /gene="xerS"
                     /locus_tag="ACHARQ_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8409408.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23946..24179
                     /locus_tag="ACHARQ_000969"
     CDS             23946..24179
                     /locus_tag="ACHARQ_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8409409.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24176..24583
                     /locus_tag="ACHARQ_000970"
     CDS             24176..24583
                     /locus_tag="ACHARQ_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8409410.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25720..26238)
                     /locus_tag="ACHARQ_000971"
     CDS             complement(25720..26238)
                     /locus_tag="ACHARQ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8409411.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26605..26853
                     /locus_tag="ACHARQ_000972"
     CDS             26605..26853
                     /locus_tag="ACHARQ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409412.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            27356..27745
                     /locus_tag="ACHARQ_000973"
                     /pseudo
     CDS             27356..27745
                     /locus_tag="ACHARQ_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         9518 a         4220 c         5125 g         8882 t
ORIGIN      
        1 tatactacca tttgttggta gtataattta aaggtaataa gttattcttt tattactatg
       61 tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac aatattaatg
      121 aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag acaaaatgat
      181 agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag tccattctgt
      241 aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc caattttact
      301 acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt tcgatgtcat
      361 ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct tgattatgaa
      421 aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta ttcacgttca
      481 ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa tggtaagttt
      541 tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt
      601 cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga aaaaatgtac
      661 gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc tgaagatatt
      721 attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat ggatgctact
      781 cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt aattggcatt
      841 cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga atctgcttat
      901 gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt tttattagtc
      961 gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc aaatgctcaa
     1021 tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat
     1081 cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa agaagaagca
     1141 aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg agtggttaaa
     1201 ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat cagaagaact
     1261 atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata taccaaaaga
     1321 aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca attcaacaac
     1381 tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca tgatacatta
     1441 gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa aattattgac
     1501 gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa atattttatt
     1561 aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt atagtaaggg
     1621 gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt aaattagcta
     1681 ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct tagaatcttc
     1741 atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt tactgaaatg
     1801 atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt atatatataa
     1861 gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt taagaaggga
     1921 aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac aagattttca
     1981 gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt tgtttggaat
     2041 caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata tacgaggcaa
     2101 aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt ttatagataa
     2161 tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt ggttatttcc
     2221 gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat atgcagatat
     2281 agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa cttttgggta
     2341 tcactactac aagaaaacta gagacattgc taccttaatg tttattttta atcatagctc
     2401 gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag catcacttcg
     2461 tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac aaagattaaa
     2521 agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa gctggggtta
     2581 ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca tttttaggtt
     2641 ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca aacgctgcgg
     2701 aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga tagtctgctt
     2761 ttttcgtttt tgaccatact tttgatcttc tgcaaacttc cttgcttttc ctctgtagaa
     2821 ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc gatatgggtg
     2881 tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata cacttctttg
     2941 acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc atgaagtgtg
     3001 atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac atagtctaaa
     3061 taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt tataggtttg
     3121 attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg tgaatcgctt
     3181 tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac taatgaaaac
     3241 aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc accaccgtgt
     3301 gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt ttggaaccaa
     3361 tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt tttgggaaat
     3421 atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg gacgacctat
     3481 cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac gcgaatttga
     3541 agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc atgccatttt
     3601 aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac gagagttatt
     3661 gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc aacgagtgac
     3721 aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc aagatcgtga
     3781 gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg gcgtagaaaa
     3841 taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta taaattcagg
     3901 aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc aattattgat
     3961 tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc aaagtccacg
     4021 ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg aacaagtgga
     4081 gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat aagtcaaaaa
     4141 ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg ttcctatttt
     4201 tataaactat cattttcgac taataaaact acttatgagt cagcctctct ttttttcttg
     4261 atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg tctcattgaa
     4321 acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata ttgaaattat
     4381 ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat aaaattagta
     4441 tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact attacccaag
     4501 aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt ttacctaaat
     4561 acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg gtcaatcaaa
     4621 agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc aatgcgttat
     4681 ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat gcttattcat
     4741 ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg attgcatttt
     4801 taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt tctgatccat
     4861 ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt tattttgtat
     4921 tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat tggcgttttg
     4981 aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat aacgtttttg
     5041 atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga gaacttgaac
     5101 aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca ttggcagtgg
     5161 aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac tacattgtag
     5221 gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta aagaaatatc
     5281 atttaacctt taaatcgcat tggatatata acaactctga atttgagcga atttatcaag
     5341 aattagctga atttactgca attgttgtta ctggagatta tttagcggct aaattagtaa
     5401 aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt ggatttgatg
     5461 atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta aatccaggta
     5521 acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa gaaataaaaa
     5581 atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac aaaatcaaat
     5641 aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat agagagaatg
     5701 aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat ttactctaaa
     5761 ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt taacgaaatt
     5821 tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat gaaaagagga
     5881 gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat tgcaacagta
     5941 tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa acgaaagatt
     6001 gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa aaatttaaca
     6061 aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc ctattttcga
     6121 acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt gaagaaaatt
     6181 attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca aggtgtgttg
     6241 attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa ttcgaatgtc
     6301 gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac tgatttaaca
     6361 aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa cattgcgttt
     6421 atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc agatgatgat
     6481 tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa atatagtcaa
     6541 atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt acttttgaaa
     6601 aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc tattgctgtg
     6661 ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat ttcgattgtg
     6721 agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac ggtgaatgtg
     6781 aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat aaaagaagtt
     6841 agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga aagtgaaaaa
     6901 aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt ttactaatat
     6961 ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt taacaaaaag
     7021 aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata gctttaacag
     7081 ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat agtaattcta
     7141 aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct ccaggactaa
     7201 gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa atggagacaa
     7261 cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt aatatgccag
     7321 atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat gctttgatgg
     7381 gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact gaaggaattg
     7441 ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct tctgtagtgg
     7501 ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca gatgaaacat
     7561 ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca aaaggcatct
     7621 atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt tatcaaaatg
     7681 ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca gaagcacaag
     7741 aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca acaattacag
     7801 aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca atgatgattg
     7861 aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa aatgttgctt
     7921 ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt ggttgggctg
     7981 ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt ttagctagtg
     8041 aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta aaaggctttg
     8101 aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga aaaatgttgg
     8161 agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa gcagaagttc
     8221 aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc gctcaaattg
     8281 aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac aatatacagg
     8341 cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg ggatattatg
     8401 aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta tatttttatt
     8461 tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca aacagcaatt
     8521 ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt aagaaattac
     8581 caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt aatttatgca
     8641 gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt aatgaataga
     8701 aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt tgctgcacca
     8761 tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact gattaataac
     8821 atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc gattgcgatt
     8881 tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt gttattacta
     8941 gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga tggtgctagt
     9001 ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt attttttgta
     9061 gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat gatgattact
     9121 cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta taaacattca
     9181 tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt agtgattata
     9241 ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc
     9301 tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat gcttatttta
     9361 ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg gaaaacgttg
     9421 gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct aagtaactat
     9481 caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt tttaatggtt
     9541 gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt
     9601 ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat gatgattccc
     9661 ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat tttaaataca
     9721 attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt attaagacaa
     9781 tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg tgccaatatt
     9841 tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt tgcacttgga
     9901 atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt caatacttca
     9961 attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata tgccacgaac
    10021 ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt gatattcatc
    10081 gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt ataaaactat
    10141 taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg caggaagtac
    10201 tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac aagatgcaca
    10261 tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa
    10321 tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga
    10381 agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc
    10441 aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta ttggtgacac
    10501 catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt tagactttgc
    10561 taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa atccgatggc
    10621 gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt tatgtcatag
    10681 cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc aatatgactt
    10741 aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt tggaaattac
    10801 aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc
    10861 acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata ttacagaatc
    10921 aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc cagaattgat
    10981 tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac aaattaatga
    11041 ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata cgagaagccg
    11101 tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag tgattgcagg
    11161 aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg tggaagtacc
    11221 ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt
    11281 agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag ccgctttaac
    11341 actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat catctgaatt
    11401 gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg gggactattt
    11461 acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc ttactctttt
    11521 cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac attagtgtta
    11581 acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt ggtctaaaaa
    11641 aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt gtggaaaaaa
    11701 tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa aagaaatgac
    11761 tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata ttcatgtaaa
    11821 agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg caggcgatgt
    11881 accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg cgaaagctgg
    11941 ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg gatatgcaca
    12001 acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg tctatggttt
    12061 ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa cttgggcaga
    12121 atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg cgattgcagg
    12181 agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg ctgctggtgg
    12241 tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta atgctgaaag
    12301 caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa aagctttgca
    12361 aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa aaggtgatgc
    12421 gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa atccagattt
    12481 tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa cgattggcgc
    12541 aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag ccaaacgttt
    12601 tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag atggtgcacc
    12661 gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg gtttaactga
    12721 acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata gtgaaaatga
    12781 tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca tgattaacgc
    12841 attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata aaatgaaaat
    12901 aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa ttattttgca
    12961 gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa ctaattggac
    13021 tggtctgact tataattttg attttgtggg tttgaacaat tataagattc ttttttctga
    13081 tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca tgattgctgg
    13141 acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta aagggaaaac
    13201 attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg tttctttagt
    13261 atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag gcattgaatt
    13321 tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa tttttgtttt
    13381 actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc aaagtattcc
    13441 acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa ttttttggaa
    13501 tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg cattaaaagg
    13561 tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa gcgatgccac
    13621 aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt ttggttatgc
    13681 gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg cacaattaaa
    13741 attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa agaaaaaaat
    13801 atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc cttagtttca
    13861 acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt tcgttttcct
    13921 aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg tcattacttt
    13981 tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat tattcctatg
    14041 gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt gatgtattta
    14101 tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat taccgttatg
    14161 atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct cacttatgcc
    14221 gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc tatcagtctg
    14281 gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa aattgttttc
    14341 ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg gttttggaat
    14401 gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac actaccattg
    14461 tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt tgcttcctat
    14521 acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag tattatagct
    14581 ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa aggagttcat
    14641 aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa taaaaaaatt
    14701 agctatctat taggaatcga agaaaataat tatttaacgc atctttattt tggaaaaaag
    14761 attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt ctcacccaac
    14821 aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga atttccagga
    14881 tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa cggtagtcgt
    14941 gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag cttagcggga
    15001 ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat tcaattgaaa
    15061 gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa tcacagtgta
    15121 attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat taaccgctta
    15181 gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt aaatggtacc
    15241 tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa agtattagat
    15301 agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt agatgccaaa
    15361 acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg taatcacgaa
    15421 acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat taattcattt
    15481 aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt cgtgatggtt
    15541 tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta taatcaccgt
    15601 ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa ctgggaagca
    15661 acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc gaagaacttg
    15721 ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa tgacttcact
    15781 tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga aaatttagcc
    15841 aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga aatgatttct
    15901 gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc aggtcgtgac
    15961 cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt tcgtgataat
    16021 atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt gaagtgggat
    16081 atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat gcaaggtgaa
    16141 gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac aacgaattat
    16201 ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc aggtttcttg
    16261 tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg tctgaaaatt
    16321 caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt ttcagcagtc
    16381 ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc tgcgatggct
    16441 ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa agcagaaatt
    16501 aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg aactttttat
    16561 cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc aaaagaccaa
    16621 acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc attagtcaca
    16681 ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt aacatttgct
    16741 ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg tgattatgcg
    16801 acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata agtgcaacac
    16861 caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt tactaaaaag
    16921 cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc aatgattaac
    16981 tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa catcaaaaaa
    17041 aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt cattgggccc
    17101 tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga gcggacattt
    17161 aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg tttagatgaa
    17221 tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac ggatattgat
    17281 gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga tatgcgtacc
    17341 aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt atatttagaa
    17401 aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat caatgattat
    17461 agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt tatttatcaa
    17521 ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt tgatgatgta
    17581 gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga gcaagcattg
    17641 aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg gaatacgcaa
    17701 aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc taattatcca
    17761 gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta ttatcatcaa
    17821 ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg acaatttcta
    17881 ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga agataaaatc
    17941 gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga tgaacagtac
    18001 caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg aaaattgcgt
    18061 ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg aaaattaaaa
    18121 acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc gaattaaaac
    18181 aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg cctttttttc
    18241 catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat ccagctcttg
    18301 gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt gattttatga
    18361 ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac catgagcagt
    18421 caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat cgaccaacac
    18481 aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa acggtagaat
    18541 ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa attgatttag
    18601 atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat atggcgaacc
    18661 atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa ttagatacca
    18721 atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt caagaagcag
    18781 cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt caaatgaaaa
    18841 ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact ctgtattcac
    18901 tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg atgaagcagt
    18961 ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat ttattaacag
    19021 atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat gtgaagaagg
    19081 tctattcaag tgccaattac aataatttag atatttatca aattaacagc acctattata
    19141 gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa gtatttgcgc
    19201 cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat attgaactgt
    19261 tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta gccgaaattg
    19321 aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa tttagaaatg
    19381 aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca gaatcagaaa
    19441 tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg gctaacttgg
    19501 atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt gttgcattaa
    19561 aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga tgacttatcc
    19621 aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc atttaataaa
    19681 tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg tatttttgat
    19741 tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt tctctacgca
    19801 gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga ccgatcgctc
    19861 caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc atttaatgca
    19921 cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt tcaacatcaa
    19981 atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt ttcgcttagt
    20041 ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa gttacactta
    20101 aactattgtt atatggacga tactacgatg tatccaactg attttacctt aacaaatgcc
    20161 catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc aacttacgtg
    20221 tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga tggctatttc
    20281 ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct ggaaacatcg
    20341 ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac ctatctaacc
    20401 gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca atttatcacc
    20461 aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg ttggcaaatc
    20521 gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt ttctcaaagt
    20581 gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct cacgtttctc
    20641 atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt ctttcgttat
    20701 ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg agaaaaaaga
    20761 aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt tctggaaaaa
    20821 gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat agttttcaga
    20881 atattctaaa atgttttatg caacactagt gatatttttt agaattagat ttctaatgtg
    20941 cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt aaatttgaat
    21001 aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt gaataaaaaa
    21061 tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg ttatgggaag
    21121 tatcagtctg agagaatcat acgacaggca aaagcaatta tggcttcaaa aagctatacc
    21181 tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga agttttaggt
    21241 atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa aagataaaaa
    21301 tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga aaagagtaca
    21361 aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc gaacttgttt
    21421 ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag gagttttttt
    21481 ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat aaaaatcggt
    21541 tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag aaatacgtcc
    21601 aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt cttatgtaag
    21661 aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg gactacttga
    21721 aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag ttgatttttg
    21781 gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt tgtaccaaca
    21841 ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg gagaagccac
    21901 tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga ataaaacact
    21961 ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt ctagtattcg
    22021 tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg agcgtcaagt
    22081 ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc agaaatatac
    22141 aatccgtcat attatgagca gacatgcaac gatcgttggt gttcatataa tccgcataca
    22201 cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg cacttcgaat
    22261 taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg gtcatttgta
    22321 tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa ctaatttttc
    22381 taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca aaagtaggat
    22441 cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg tgttgtttct
    22501 tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat cgtagccgca
    22561 ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa ttgttgggca
    22621 gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg acgcagtttg
    22681 tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg ttcgattgca
    22741 ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc tttttcgttt
    22801 tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata ttccattgcg
    22861 aatgctgcta tattgacagc atctctttta tttccttttc tagttactac cacattattc
    22921 ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct caatccagat
    22981 cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata atagatcaaa
    23041 gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata attttgtgta
    23101 tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa cgtagtttta
    23161 tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt tcctagagga
    23221 ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat atttctattg
    23281 atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt tgttgtctct
    23341 ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt taggtcatag
    23401 gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc actcagaagc
    23461 cattcataaa agagcttata attttttaga tattctaaag tggttcgagg tgattgatct
    23521 ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag tatgattgta
    23581 tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat aagtagggga
    23641 gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa ttggtatgct
    23701 ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt taataattta
    23761 aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa attttttgaa
    23821 ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca tgagagggac
    23881 tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa aggagggaga
    23941 agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta gtattaacta
    24001 tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt tttttagatg
    24061 aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa attgatttag
    24121 caatatctca agcattaact gaatatgata ctacttttag agagcttgtc gataaatgag
    24181 tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa taaaaaaata
    24241 ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt gtgtagggca
    24301 acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa aagcagctat
    24361 cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac gtactgcttt
    24421 cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg atcaaaaaga
    24481 tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc aactaaaata
    24541 tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta ctgtatgtgt
    24601 ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa atagctataa
    24661 ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa gataacgact
    24721 atttatttta tcatattttt actataccct atacttttac ttggtatatt tatgaataat
    24781 cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata ctaaagttat
    24841 ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga taacacgaaa
    24901 gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat cgaaagtgag
    24961 ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt acatcttgca
    25021 gaaatcgtta ctgggattaa actaagcaat aaaaactaat gttcccctca ttctttgaac
    25081 ttctactatt tgatttattt attctgataa atgacgattt caccttcagg atcttcaaat
    25141 cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc gataggccac
    25201 ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg gctttagcat
    25261 attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct taacaatttc
    25321 aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac ccttcatgtg
    25381 ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc tgaaaaacat
    25441 cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta gcggatatgg
    25501 caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa ccaaaattga
    25561 tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca ttcacccaat
    25621 aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt aacatacaag
    25681 aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta atctcttcca
    25741 ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat gaataaattt
    25801 atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt tgtgtgcagt
    25861 gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa tattacgatt
    25921 gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa accacttata
    25981 taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag ataatccatt
    26041 tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat atgctaaatt
    26101 ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt caggaatagt
    26161 ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta attcttcttt
    26221 atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc tttaattcat
    26281 ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat atgcattgac
    26341 tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta aagaaatgtt
    26401 gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa atcggactgg
    26461 tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca attactaata
    26521 aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga tagttcacaa
    26581 aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat aggattacag
    26641 catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa aattgacgat
    26701 gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga tcaactcgaa
    26761 gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg aactattaaa
    26821 ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta tgttgcacaa
    26881 tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg cggtgtaatg
    26941 aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat gaataattat
    27001 gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta tagattgttt
    27061 aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata ctctgttaag
    27121 ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac taacggacag
    27181 tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa atgctgctca
    27241 tttcaactta tatgttttag tgaactaccc ccacttagct tggataggct acaattaact
    27301 agacagaaaa attaaggtgt gtagactaga agaaaacata ccaggaggaa tttttatgtc
    27361 taagagaaca cgaagaactt tttcacaaga attcaagcaa caaatcgtca atctttactt
    27421 agctggaaag ccacgtgtag aaatcattcg agaatatgaa ctaacggctt cagcatttga
    27481 caaatgggta aagcaatcta aaacgagtgg ttcattcaaa gaaaaagata atcttacgcc
    27541 tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa tggaaaatga
    27601 tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga tgcgaataag
    27661 catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca gtcctattat
    27721 tatcaatcaa aaccaaagaa agacg
//
LOCUS       DAYYAG010000026        27736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000026 DAYYAG010000000
VERSION     DAYYAG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.026"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..1197)
                     /locus_tag="ACHARQ_000974"
     CDS             complement(253..1197)
                     /locus_tag="ACHARQ_000974"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8409413.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1493..2437)
                     /locus_tag="ACHARQ_000975"
     CDS             complement(1493..2437)
                     /locus_tag="ACHARQ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8409414.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2658..3872
                     /locus_tag="ACHARQ_000976"
     CDS             2658..3872
                     /locus_tag="ACHARQ_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8409415.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3894..5909
                     /locus_tag="ACHARQ_000977"
     CDS             3894..5909
                     /locus_tag="ACHARQ_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8409416.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5992..7365)
                     /locus_tag="ACHARQ_000978"
     CDS             complement(5992..7365)
                     /locus_tag="ACHARQ_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8409417.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7391..7675)
                     /locus_tag="ACHARQ_000979"
     CDS             complement(7391..7675)
                     /locus_tag="ACHARQ_000979"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8409418.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7677..9692)
                     /locus_tag="ACHARQ_000980"
     CDS             complement(7677..9692)
                     /locus_tag="ACHARQ_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8409419.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9871..10176)
                     /locus_tag="ACHARQ_000981"
     CDS             complement(9871..10176)
                     /locus_tag="ACHARQ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8409420.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10265..11296)
                     /locus_tag="ACHARQ_000982"
     CDS             complement(10265..11296)
                     /locus_tag="ACHARQ_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8409421.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11503..12378
                     /locus_tag="ACHARQ_000983"
     CDS             11503..12378
                     /locus_tag="ACHARQ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8409422.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12530..13372)
                     /locus_tag="ACHARQ_000984"
     CDS             complement(12530..13372)
                     /locus_tag="ACHARQ_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8409423.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13464..15341)
                     /locus_tag="ACHARQ_000985"
     CDS             complement(13464..15341)
                     /locus_tag="ACHARQ_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8409424.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15338..16759)
                     /locus_tag="ACHARQ_000986"
     CDS             complement(15338..16759)
                     /locus_tag="ACHARQ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8409425.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16762..20151)
                     /locus_tag="ACHARQ_000987"
     CDS             complement(16762..20151)
                     /locus_tag="ACHARQ_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8409426.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20408..21799
                     /locus_tag="ACHARQ_000988"
     CDS             20408..21799
                     /locus_tag="ACHARQ_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409427.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21872..22519)
                     /locus_tag="ACHARQ_000989"
     CDS             complement(21872..22519)
                     /locus_tag="ACHARQ_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8409428.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22527..23213)
                     /locus_tag="ACHARQ_000990"
     CDS             complement(22527..23213)
                     /locus_tag="ACHARQ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409429.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23219..24292)
                     /locus_tag="ACHARQ_000991"
     CDS             complement(23219..24292)
                     /locus_tag="ACHARQ_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409430.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24514..25188
                     /locus_tag="ACHARQ_000992"
     CDS             24514..25188
                     /locus_tag="ACHARQ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8409431.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25193..26533
                     /locus_tag="ACHARQ_000993"
     CDS             25193..26533
                     /locus_tag="ACHARQ_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8409432.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26682..27668)
                     /locus_tag="ACHARQ_000994"
     CDS             complement(26682..27668)
                     /locus_tag="ACHARQ_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8409433.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8082 a         5244 c         5037 g         9373 t
ORIGIN      
        1 tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc acagataaac
       61 attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc atatgtacgg
      121 atacattttc ggaccgatta attttaacca ttctatttat ttgcttacct attagatatc
      181 aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt tttagttcat
      241 attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg ttgatttgct
      301 gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa caccattttc
      361 gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac cagttaaata
      421 agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga tacgtgcagt
      481 actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat aagcagcatt
      541 acggacttta tcagcaatca catctaattc atcttgggca atacgatggt ctttttctac
      601 aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa cttctgaatc
      661 accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta atttcaatgc
      721 gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa cacgtgatgt
      781 tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt ttgaagcaac
      841 aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc gcatgattga
      901 cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga taccagctgt
      961 gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata cagccacatt
     1021 ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg ctttttcttg
     1081 attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg cataagcgat
     1141 acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt ttttcatgaa
     1201 taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc ttgtttattc
     1261 attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga aacgaattcc
     1321 tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga taatacaacc
     1381 aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata ataagacagc
     1441 aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt
     1501 tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag
     1561 tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa
     1621 tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag gaagcatcaa
     1681 aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt
     1741 cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat
     1801 tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga gtcaattcga
     1861 tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat
     1921 aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat
     1981 gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa gaagaaagta
     2041 gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata
     2101 agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat
     2161 caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata atttttaaac
     2221 atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg
     2281 catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga
     2341 tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt
     2401 cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa ttcagctagt
     2461 tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact
     2521 gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac
     2581 ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt
     2641 acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag
     2701 tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta
     2761 cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg
     2821 tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg
     2881 tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt
     2941 acagtattgt cttagcttca agagctggac taggtattgg ctttggattg atcaattctt
     3001 tggcagtcag catgattgct gccttttatc acggagatga acgagcctct atgattggtt
     3061 ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag
     3121 tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct
     3181 tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc
     3241 caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc
     3301 tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt
     3361 ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg
     3421 ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt
     3481 ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga
     3541 ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca
     3601 accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg
     3661 ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt
     3721 ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg
     3781 cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg
     3841 aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa
     3901 atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac
     3961 tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg
     4021 atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt
     4081 ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg
     4141 gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat
     4201 cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag
     4261 caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa
     4321 aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga
     4381 ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat
     4441 ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta
     4501 tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta
     4561 aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag
     4621 tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac
     4681 tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta
     4741 tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga
     4801 tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag
     4861 gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga
     4921 ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc
     4981 ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt
     5041 atgggagagc aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc
     5101 ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata
     5161 tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat
     5221 atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta
     5281 tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt
     5341 tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg
     5401 acactatcac acagattact actccatatt tcaaattcta tgaacaagat gctttatgca
     5461 aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat
     5521 ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt
     5581 acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa
     5641 tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat
     5701 ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag
     5761 atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag
     5821 gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct
     5881 taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga
     5941 ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg
     6001 ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag
     6061 taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc
     6121 aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag
     6181 atctcctaat actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc
     6241 agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc
     6301 tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat
     6361 agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt
     6421 aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt
     6481 tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag
     6541 gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt
     6601 gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac
     6661 atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact
     6721 tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca
     6781 tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt
     6841 gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa
     6901 gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac
     6961 tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct
     7021 tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg
     7081 tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg
     7141 agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc
     7201 agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc
     7261 tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga
     7321 gggctgactg acgattgaca caaacaaatt caacaattta tccattgata gttcctccct
     7381 tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt
     7441 caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca
     7501 aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat
     7561 gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca
     7621 tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat
     7681 tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct
     7741 tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc
     7801 tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc
     7861 agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct
     7921 ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc
     7981 acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga taatagcggc
     8041 tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt
     8101 tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg
     8161 ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg
     8221 atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc
     8281 agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga
     8341 tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata
     8401 atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa
     8461 aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca
     8521 cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg
     8581 atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct
     8641 attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga
     8701 tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag
     8761 gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg
     8821 gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt
     8881 gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc
     8941 tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg
     9001 actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt
     9061 cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga
     9121 agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg
     9181 gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga
     9241 gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca
     9301 gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg
     9361 atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa
     9421 acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta
     9481 tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat
     9541 ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc
     9601 gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt
     9661 gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct
     9721 agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt
     9781 acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt
     9841 ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt
     9901 cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg
     9961 attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc
    10021 tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc
    10081 accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct
    10141 taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat
    10201 actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa
    10261 tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca
    10321 tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt
    10381 aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat
    10441 aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa
    10501 agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt
    10561 gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct ttgatgcgga
    10621 aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga tcgaagtaat
    10681 cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca tctggttcgc
    10741 cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa tagctgttcg
    10801 cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca taatgcattt
    10861 ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact gtcaagaagt
    10921 cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct tttgctaatg
    10981 caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact agtacattct
    11041 taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt gtagtgatga
    11101 caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta tattcgatgt
    11161 cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata ttgattttgt
    11221 tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca ttgccaagat
    11281 aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat atcctagaaa
    11341 agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa cacttgttat
    11401 cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag atagtagcgg
    11461 aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat tgaagaaaaa
    11521 ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta catccgtagg
    11581 aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac gtatacttcc
    11641 cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa agaattccgt
    11701 ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac gattgatcct
    11761 aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat ggcggattta
    11821 tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt agatcaaatc
    11881 ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga ttctcaaatc
    11941 acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat catggctgaa
    12001 gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg cgccattttt
    12061 attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt gaagcatgta
    12121 gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa acaggctgat
    12181 atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac tttctcctct
    12241 caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc tcaaaatttt
    12301 gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg ccttcttaaa
    12361 gaatatcgga aaacttaaga aaagatcctc atttagtaat aagaaagata gtgtttgata
    12421 ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa cctcagcctg
    12481 tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc taacttctgt
    12541 ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc agtatacatg
    12601 cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt acttcttttt
    12661 gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga tgactgttga
    12721 ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg attctcagat
    12781 cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg agtgtccgtc
    12841 cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct gtgaacaact
    12901 ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct ccccccgtag
    12961 gataagacgt tccttccaat aatgaggcac ctttttctag cagcacagta ttcgtttcat
    13021 caaaaattgg caaatggaca ttgatctcag ggatcgtcaa aataccgatc gtatgtttct
    13081 caaaataggt tctatccttt ttcgcaggtt cttttttctt agtaaaggga tctgcacctg
    13141 gatttccacc ttcttttgct aattgctggt ttttctttgt catgttttct tggattttag
    13201 ccatgacttc ttcattttct ttgaccgctt gttgctgata atgagagatg atctgctgat
    13261 ccagataatt attcagcgcg tcgctaacaa atggataagc tagtatccct attcctgaga
    13321 gaagcaagaa aagcatcagc agatctagaa gtttcctttt ctttggtttc atttgtgttc
    13381 tccttgtgat gactgtttga taataaaaga aaaaggagca tttgcgtaat ccgctgctcc
    13441 tttttccaat tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc aaagtacagt
    13501 ccagcaatca gtagaaggac tactcctgca ccgatataca catagattcc cttaccgcct
    13561 gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt caggagaaat caactgtcct
    13621 gctgttacat aagattgttc atcgatagta aatgctacac ggtttgttaa tggaacatat
    13681 ttttctggcg ctttcgtttc ttccagatag tacgtgccat attttagacc tgtcacatcg
    13741 attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga tacccagctg
    13801 acggcttttg tggatgggtc gatcgataaa tatttcgctg tgtcactatc ttgatcacga
    13861 acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc tactttaaca
    13921 aatcgtttgc ccccagtaac gacatcgact gacggtggtg tttgatcatt tgtatgcccg
    13981 ttatcgacat ttgcttggtt agaaaatcct ttggttggat ctgctttttc gttcaaatgc
    14041 atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg aaggaatata attcggatca
    14101 actgaaaccg tgaatccgtc tgtttgctca gtgacagaat aatttactgg gtcgatttct
    14161 ttatttccat catataaggc ataagcatac gttcctgaag aatcattatc aaatgttaaa
    14221 gcagcgtcat gagtatcgat aagtttgaat gttgtgtact tgttttgcgt tccttcttta
    14281 tcagcgatac ctaatgggat attgacagaa atctcatatt gaattttttc accgattgcg
    14341 acatcttttc cattcaattg aggtgttgtt ttatcaactt tagacgtatc atttttgatg
    14401 gtcttttcta ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat tgtttgcttt
    14461 tcaatcattt ccgcattatc aggagctttt acttcttcta aattatattt tccaacttct
    14521 aaatgattaa cgatcaactg gcctttttca atagatgcct cggcaaagtc attgttgccg
    14581 atgtcataag aatgaccagt aatgaaatgt ttggctttgg tttgatcagt tgtccaagtg
    14641 tacaatccat ctgtgacact ttggatgtat tttttgacgc ttggtgttcc ttcttcttta
    14701 gaaataataa attctgctcc atttagtgct tcgttttcgg cagtaccgat ttttgtaact
    14761 ttcaacgttc catcattacc gactgtattt ttagggtaga gatggatagt atctagttct
    14821 tctgtcccgt atttataaga gccatctgct tgtttgatca tctcatatac agggaaagct
    14881 aaaaccatgt ttgcggcagc tgacactccg tcttttggtt cttctttgat cgtgtagact
    14941 gcatctttcc cattttgttt tttaggtaaa gataaagtga catttccatc agcatctgtc
    15001 gttcctgaag caactggtgt accaggagtc aaagactgga ctgcttgttt tgctgcatcc
    15061 acggacgctc ctttatctcg ttgcgcataa aattcttgag tgacgttata aactgaaaat
    15121 gaaatatcgg ctaatccttg gtattgatcg aattcgctca tttctttccc gctgttttgg
    15181 attaaaggat cgggtaaatc agtcattttc tttttatgaa ggatgacttg agctgctgtc
    15241 tcctctgcaa acactttttt tgcgccgaag ctgtttgtga gtaatggtaa aataaggaaa
    15301 aggactccta acataacgtt tattttttta tgatttttca ttttttcact cctgttcttt
    15361 ttttgataaa caaccagatt gcaaagcttg cgaataccat accaacaacg cctaaaagtg
    15421 tatttttcgt ttcgtttgtt tttggaagaa atggacggct attttctttt ttattgacta
    15481 ctttcaatat actgtccgcc tctttatcct cagaaagaaa agttgaaatg gtaaacggaa
    15541 tttctgtttc acttaaaata taaccagaag gtgcttcgat ctcttttaat cgataacttc
    15601 ctgttttcaa cccggaaatt gaaaaagcgc catttttatc ggaaatcagg tgaagcgcat
    15661 ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa tttcctgctt
    15721 tgtttttaat aaggaatact gcgccagcta atcgtttatc tggtttattt gtctcgattt
    15781 tgacaaattg cttactccct gtatatacag agactttctt tgtactgaca ccaaaaccaa
    15841 aatccagtgt tccgttgttg ttgatttcct tgttcgcctg tgctgtactg cttagctgca
    15901 tctgataaga aatggtcagt ttttttccag catatttttc caacgaaggg atcgaaaata
    15961 aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc ttttctccat
    16021 tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca ctatctgaca
    16081 attcgaactt ttgatagtca aggatattta caggaattgt cgtatgcaag gaataagtga
    16141 ttttttcacc aatcgtaaaa tcatttctag gctcttctaa taccttctca aaaggtggat
    16201 cagggtagtc gttttcttca tttttaggat acaaatggat agttgaaagt ttttgattgt
    16261 tttgtccatg tacaggaaga acaactacca tattctctgc cttttctttg acgacttctg
    16321 gtactttgga ttcaatgaaa agatacgctt tatctctttt ttcttgatct ttagcggcca
    16381 gagaaaaaga agcgatacca tcttcgttgt taagagttgt agttgttgct tctgcggtaa
    16441 acataccgga agacgcaccg ttttttgcga tctctgcttg tgcttcttct accgttttgc
    16501 ccttttcccg tagatggtaa aaagaatctg tgacatcata aacttggaat gtgacaccat
    16561 ttaatcctcg atacgtttgt aataaagctt tttcttgtcc gtcatttgga tgatttttcg
    16621 gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt tctgtttctg
    16681 tttctgttgc tacctgagta aaagctggtt taaatagtaa caagaagaga cacatactaa
    16741 gccaaccaag ttttttcata tttagctgcc ccctctttct tttttcaaaa aaagatacca
    16801 aatagaaaac gcacaaccaa tcatccctac tagatagatt cctaaacggc caattcctcc
    16861 cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct aaagaaatct ggttgttttt
    16921 ggctcctggt gacagaacag attcatgatc ttgtccatcc acttgaattg acccatcttc
    16981 gtgtataact atagtaactg gctcttttag accttggtat ccttctggtg taaaagtttc
    17041 ggtcagcgta taagttccag gttttaaatt ttcgaataga aaggtatctg tttcttgtcc
    17101 atcttttggc gattctaagt ccatttctgg tccctgcagt ctgaattttg ctccttttaa
    17161 tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca aacgctttta gtcgattttg
    17221 atgagtgagc gtccatacat tatttgtcat ctcggtattt tttcccgtgt aaatgatttt
    17281 tccatattta tcgatttgga attcataatt tcccggcgaa gtatcatatc cggcaggacc
    17341 agctgtttca acgactcgat atttaccagg tgtgagtgaa tcaaaactgg caagaccttt
    17401 ttcattcgtt gtttggctgt caattggctg ataagtacca tttgcttctt ttttctgtag
    17461 ggaaaatgct gctcctttaa gatttatctc tttgtccgtc ccttgcatcg catacttacg
    17521 tactgctaca ggaacttcaa caaaaggatt ttcaatagtc aactggatcg tatcatcgga
    17581 agtagtgact gtttttccat caatggttac cgtaccatca gaagcgatct tgatttccca
    17641 agtagtcttt ttgcttaatc catgcccttc tggagctttt gtttctgtca gcgtataggt
    17701 catttctttt tgcaatttga cattttctgg aagagaatag gttccgtcgc catgatctgt
    17761 tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa gatccttttc
    17821 acctgtagag gaatttttcg ttattttcaa gtttaacggt gtgaattgtt tagtattttt
    17881 ccatgtcatt gcatctattt gttgagaatc gtatcctggt acaggtaatt ctttgattgt
    17941 ttggtaactg aatgcttgac cttgattatt gtattgaggt agtgataata tctcttgata
    18001 actttctccg ctatttgcag ataatttgtc aatgtctgca cgttcccacg tatttgttgt
    18061 atctttagct ggtttaatga ttcgaatata tccgtttttc caagctgcag catttgtcgt
    18121 atgttcccgt tgaatctcaa aagtaacttg atctggacga tcagctagat tgttgtcaaa
    18181 ttcttcccat aactttttga tgtgaagact gactccggga gctttagcag atggtatacc
    18241 gaattcagct aattcattgg tatcaatgtt tggctggaaa gttgtcctgc cgttcatttg
    18301 ataccagaaa tttggatgga agtcctcatt ttctgtttgg attctcactt ggtaatggat
    18361 ttggatttct tggtcttttc ctaaatagat ctgattgctc ttgatggtat ttccttctat
    18421 ggaaatagat ggagtgaccg ttgtaggact tgtccccaca ctcttgactg aaagtgtacc
    18481 aggctgataa atgaatggtt ctgcgattgg atcatttatt tgtccattgc ttacagttgc
    18541 tgagatctgt acagcttttt tggctaggta ttcagagata tctgttgcat gatcagctga
    18601 ttcatagtaa agatcgcctt tttcatctga tgaaaccatt tgtcgcatac gagactctac
    18661 ttctgctttt gagagaccag ctgccggatc gctttgaagt tggatgccaa gaccatgtat
    18721 ttcgattcct cgttctttga gtgccatcgc ttctccgatg gttgcgataa acgtactgtc
    18781 gattcttctg gatagattgt tttggtcagg tgcatcatag attcttgaga ttagagaagt
    18841 atttcccggc cgatcttgcg tattagaaaa ctgagttccg taataattgc tgcttgattg
    18901 tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca ccatccgtca gcaaaacgat
    18961 cactttttta tgtccatttg gaacggatag catgcttcct gcatctctta gtgctttttg
    19021 tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat tttacttgat ttttcactga
    19081 atcaaatgaa cccatctgta ctgcaccgtt actgtagcta tatccttcgc ttgagtagcc
    19141 gacatatccc atattgattt tgtctgtgat accgctatct gctaaagtat cgacaaaacg
    19201 atcgacacca atctttactt caccgatccg attattgtcg ttcatacttc ctgaccagtc
    19261 tacgaccaat acgagatcaa gaggcgtgat atctttttgt acatttccac gagcattcaa
    19321 ataaacatca aacaatccgg gtgtacttgt ttcctttgca tactttcgta tcgcatagtc
    19381 tgcttgatta ccggttcctc catattcgat atacgaatgg gtccgatcat taggatctcc
    19441 attccaagaa tttataccat cccattgatt tgttccgttt ttattgcctt gatgatcaag
    19501 aacatttgta tttcctgtag gttgccaact ggctttagga taagtgccct gatcatctgt
    19561 tgtatattct gtaggaatcg tttgattaga tggcacactc acagctggca aggataatgt
    19621 aggcaatgta aatgggctga cagaattttc gttttctttt gatgattctt cttctatttc
    19681 gtctggcttt ggtactgaca catctgtatc tttcttattt tctgtctcat caataatgtc
    19741 atattcttta ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg ctggatcgat
    19801 tggaaaactc agatcgatag tttgtttagt gtttgatgag atttccaacg taattttccc
    19861 gctatttacc tgataagcat agccatctcc ttcttttctt tccacatcac ttaagtaatt
    19921 ttcgactaga ccaatttctt tttctatctt ttcagcactg gtgttattcg catttagctt
    19981 gatgttcagt gctttttttc cagactgatc cgaaatgact tcagctgaaa ttgtttctaa
    20041 aactgttgga tcatctgtag tgtctgctat tgcagataat ggcacaaagt ttgataaact
    20101 caatattatc agcatgaaaa taactttcag tttcttccct gtggtcgtca tttttcccct
    20161 cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata aacaggcgtt
    20221 acataatttt atttatttta atatgataat tattatacga agggcgctaa agggaatcac
    20281 accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt aatgagcgtt
    20341 tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg aaggaaacta
    20401 tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga tgactaaaaa
    20461 agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata tcgaagagat
    20521 caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg ggtatcgtct
    20581 tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc aaagttctac
    20641 aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg agtattattg
    20701 tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat
    20761 tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa
    20821 aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact acggaagacg
    20881 cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca agaataattg
    20941 tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta ttatgtattt
    21001 tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag ctggagactc
    21061 ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa atctcttagg
    21121 aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata cgattttttc
    21181 agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa atatgtccta
    21241 ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt
    21301 aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt tgacgatccg
    21361 tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg ctggatttcg
    21421 tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt atttatctag
    21481 ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt ctgataaaaa
    21541 aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag aatatatcag
    21601 tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg aagatcagct
    21661 gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca ttttgaatgc
    21721 ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag aagaactcat
    21781 ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc ctgataaaaa
    21841 tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca atccaacgag
    21901 atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca atgagatccg
    21961 tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct tcttccgacg
    22021 attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc attggagaat
    22081 cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt aacaagtata
    22141 acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa tatttttcga
    22201 tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct tgatggacta
    22261 tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga aaatatttat
    22321 aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg acttggctga
    22381 tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc tttgcgttcg
    22441 ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg attatacgtg
    22501 gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc tgtcaagtac
    22561 ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc atgagtaacc
    22621 ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac gacttcatat
    22681 gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct tggctcatta
    22741 tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc tttaggataa
    22801 ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt tgttgaatct
    22861 tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt taaaaaagga
    22921 atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc tgctcgtttt
    22981 ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga agtcggtgtt
    23041 tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg accaatgatg
    23101 ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga gacctttgta
    23161 tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt catttttatt
    23221 aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa aacaatccgc
    23281 ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac caaaaaacag
    23341 caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa agagcaagca
    23401 gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg atgaagaaat
    23461 ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt gtcatcacgt
    23521 acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc atcgcattta
    23581 aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca atagttaata
    23641 actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt attacgatcc
    23701 cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc aaagcagtcc
    23761 acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca ggaaagatac
    23821 cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca attttatagc
    23881 ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat tcatgccctt
    23941 tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta acattttctt
    24001 tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct cctccttcaa
    24061 tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt gcatcctccg
    24121 acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct tctgaaagtc
    24181 cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata ataccgacga
    24241 tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac atttgttttt
    24301 cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag
    24361 tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag acatctttcg
    24421 ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc tttccatgat
    24481 aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt agtagaagac
    24541 gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga gataacacaa
    24601 gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga tcttgcaatc
    24661 ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa
    24721 aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga taagattgaa
    24781 ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga agaactgatc
    24841 atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag
    24901 tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga acaagttttg
    24961 cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa gggaatcatt
    25021 ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac gacgattaca
    25081 gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg attggataaa
    25141 gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg caatgaatgg
    25201 agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag catttgcttt
    25261 tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg catacagaga
    25321 aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat tagagataca
    25381 aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa acagaacaaa
    25441 catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg
    25501 gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca accaaatcaa
    25561 agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat taaatgaaga
    25621 cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga cgaatcaaga
    25681 tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt cattagcgac
    25741 ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta ttggtatcag
    25801 ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa gacaaaaaga
    25861 atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc aaaatagttt
    25921 ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa gcatcgctca
    25981 agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga caatcgctcg
    26041 gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag aatatatcga
    26101 acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt ttattctcca
    26161 taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag ttttggttat
    26221 tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt tggattcatc
    26281 tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta tcagtgaaga
    26341 agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg cgaaagagac
    26401 tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc atcatggaca
    26461 gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat tgccgttgaa
    26521 gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct
    26581 tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc
    26641 tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt tttattgcgg
    26701 tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct aacatcttct
    26761 gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc acgaataact
    26821 gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt gtcatcttcc
    26881 aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc gatgacgatt
    26941 tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg
    27001 tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc ttttagttga
    27061 attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct tgtacccatt
    27121 gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt caatggatcc
    27181 gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc agtgccagtt
    27241 tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc cattgtatct
    27301 tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc atcagaaata
    27361 tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga atcataggta
    27421 tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc accatccatc
    27481 cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag tctagatgct
    27541 gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt acgcaaccgt
    27601 tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt taaatttttc
    27661 tcttccatgt aaattcctcc tagcaaatag atttatgatt ggtatattgt tacttataaa
    27721 tttattctat aggaga
//
LOCUS       DAYYAG010000027        27419 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000027 DAYYAG010000000
VERSION     DAYYAG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.027"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1882
                     /gene="yfcC"
                     /locus_tag="ACHARQ_000995"
     CDS             143..1882
                     /gene="yfcC"
                     /locus_tag="ACHARQ_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8409434.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2039..3223
                     /gene="iadA"
                     /locus_tag="ACHARQ_000996"
     CDS             2039..3223
                     /gene="iadA"
                     /locus_tag="ACHARQ_000996"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8409435.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3334..3537
                     /locus_tag="ACHARQ_000997"
     CDS             3334..3537
                     /locus_tag="ACHARQ_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8409436.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3638..4300)
                     /locus_tag="ACHARQ_000998"
     CDS             complement(3638..4300)
                     /locus_tag="ACHARQ_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409437.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4313..5815)
                     /locus_tag="ACHARQ_000999"
     CDS             complement(4313..5815)
                     /locus_tag="ACHARQ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409438.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6363..8042
                     /locus_tag="ACHARQ_001000"
     CDS             6363..8042
                     /locus_tag="ACHARQ_001000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8409439.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8169..8648
                     /locus_tag="ACHARQ_001001"
     CDS             8169..8648
                     /locus_tag="ACHARQ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8409440.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8867..9547
                     /locus_tag="ACHARQ_001002"
     CDS             8867..9547
                     /locus_tag="ACHARQ_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8409441.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9555..10886
                     /locus_tag="ACHARQ_001003"
     CDS             9555..10886
                     /locus_tag="ACHARQ_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8409442.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10901..11863
                     /locus_tag="ACHARQ_001004"
     CDS             10901..11863
                     /locus_tag="ACHARQ_001004"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8409443.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11974..12312
                     /locus_tag="ACHARQ_001005"
     CDS             11974..12312
                     /locus_tag="ACHARQ_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8409444.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12391..13098
                     /locus_tag="ACHARQ_001006"
     CDS             12391..13098
                     /locus_tag="ACHARQ_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8409445.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13201..14682)
                     /locus_tag="ACHARQ_001007"
     CDS             complement(13201..14682)
                     /locus_tag="ACHARQ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409446.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            14991..15527
                     /locus_tag="ACHARQ_001008"
     CDS             14991..15527
                     /locus_tag="ACHARQ_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8409447.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15605..16975
                     /locus_tag="ACHARQ_001009"
     CDS             15605..16975
                     /locus_tag="ACHARQ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8409448.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17042..17377
                     /locus_tag="ACHARQ_001010"
     CDS             17042..17377
                     /locus_tag="ACHARQ_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409449.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17458..18315)
                     /locus_tag="ACHARQ_001011"
     CDS             complement(17458..18315)
                     /locus_tag="ACHARQ_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409450.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18421..18936
                     /gene="tadA"
                     /locus_tag="ACHARQ_001012"
     CDS             18421..18936
                     /gene="tadA"
                     /locus_tag="ACHARQ_001012"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8409451.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18959..19043
                     /gene="ffs"
                     /locus_tag="ACHARQ_001013"
     ncRNA           18959..19043
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHARQ_001013"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19253..20638
                     /locus_tag="ACHARQ_001014"
     CDS             19253..20638
                     /locus_tag="ACHARQ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8409452.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20675..21352
                     /locus_tag="ACHARQ_001015"
     CDS             20675..21352
                     /locus_tag="ACHARQ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8409453.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21360..22124
                     /locus_tag="ACHARQ_001016"
     CDS             21360..22124
                     /locus_tag="ACHARQ_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8409454.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22126..22785
                     /locus_tag="ACHARQ_001017"
     CDS             22126..22785
                     /locus_tag="ACHARQ_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8409455.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22815..23324)
                     /locus_tag="ACHARQ_001018"
     CDS             complement(22815..23324)
                     /locus_tag="ACHARQ_001018"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8409456.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23436..24434)
                     /locus_tag="ACHARQ_001019"
     CDS             complement(23436..24434)
                     /locus_tag="ACHARQ_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8409457.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24635..25219
                     /locus_tag="ACHARQ_001020"
     CDS             24635..25219
                     /locus_tag="ACHARQ_001020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8409458.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25296..26471)
                     /locus_tag="ACHARQ_001021"
     CDS             complement(25296..26471)
                     /locus_tag="ACHARQ_001021"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8409459.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26553..26933
                     /locus_tag="ACHARQ_001022"
     CDS             26553..26933
                     /locus_tag="ACHARQ_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8409460.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27007..27369
                     /locus_tag="ACHARQ_001023"
     CDS             27007..27369
                     /locus_tag="ACHARQ_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409461.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         8883 a         4652 c         5737 g         8147 t
ORIGIN      
        1 cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat tcatgtggaa
       61 atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca aaattactga
      121 aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac cgaaacgcga
      181 cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag tcatctttgc
      241 atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata ttgaatacaa
      301 agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct tccgttatgc
      361 ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag atcatccagc
      421 agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg ctgacggaga
      481 aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg tgctttacga
      541 tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag ccaaaatatg
      601 gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag gactagtatc
      661 tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg ttggaggtgc
      721 ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa
      781 aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt cattcggtgg
      841 cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg ttccatttgt
      901 cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg catctcaagt
      961 cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg
     1021 aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg ttactgcatt
     1081 agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt
     1141 gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg atgaaaataa
     1201 aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc ttgtctggat
     1261 cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc aattctttgt
     1321 gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa
     1381 tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag caatcgtagt
     1441 aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg ttccttcagc
     1501 tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg
     1561 tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat caaattcagg
     1621 acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag gtgtatccag
     1681 acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt ttacacccgt
     1741 atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag
     1801 attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca tcatgattgc
     1861 cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa tccaaacagt
     1921 agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa gatcctatcg
     1981 tttggattat tttttaagat cctatcatca gttatactta aagaaagagg aaaaatgtat
     2041 gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa tggatgtact
     2101 gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct acgaaggagt
     2161 ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca ttgactgtca
     2221 tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc cagaagtcac
     2281 attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg
     2341 agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag cagaaggaat
     2401 ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa cagattctat
     2461 catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg cagtatccga
     2521 tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt
     2581 aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg gaggaaaaag
     2641 aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca cacaattttt
     2701 gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat ttgccaaacg
     2761 tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga agacagatgg
     2821 tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt ctttagataa
     2881 tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa acaatcattt
     2941 cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag aagctgtgca
     3001 aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc cagcacgtat
     3061 attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc tgtgtatctt
     3121 ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa tggtccaaga
     3181 aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag ctctaactat
     3241 cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt
     3301 agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg acgttctaaa
     3361 gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc aacagacgac
     3421 aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct
     3481 cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa
     3541 ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg cgagacagat
     3601 atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt ttttgaaatt
     3661 ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc tcatgagtca
     3721 cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg acgatatctt
     3781 gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt tgatgttcac
     3841 agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt ttggtcacct
     3901 gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa gcaacttgtc
     3961 gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg tctagagccg
     4021 ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt ttacgataac
     4081 ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct tcttttggtg
     4141 catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa
     4201 tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc gcatcttgcg
     4261 gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc ctttagttta
     4321 ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag acagaaaaca
     4381 gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg acattgattt
     4441 ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct tgttgaccat
     4501 cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca ggagcttgct
     4561 ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg gcattcgcga
     4621 cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata gccacttcgg
     4681 taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag acgcctaact
     4741 catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata ataccggcta
     4801 cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa ggttccaaca
     4861 ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg atcagcttct
     4921 cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa atagcagaat
     4981 ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa
     5041 gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt gtttcgctgg
     5101 attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca gtactcgtga
     5161 ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa tcaatgacta
     5221 cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt tttgctgttc
     5281 catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga aagtctgctt
     5341 gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca tcagtagttt
     5401 ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct tttgccatag
     5461 ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct attttttccg
     5521 cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt tcttcagtgg
     5581 tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga ttggcactta
     5641 aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct gcagcgcttc
     5701 gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca aacaataaca
     5761 atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa ttcatatttt
     5821 gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct agcttaaatc
     5881 ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc actatagtta
     5941 ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa
     6001 gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca tagttgttct
     6061 aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg aacagctgaa
     6121 tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt taatgaagta
     6181 tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta attttcctgc
     6241 tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa tggctcatat
     6301 atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa gaggtgaagt
     6361 ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga aaaaacatgt
     6421 atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa tatcctgaaa
     6481 atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa gaaaacattg
     6541 atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg gcacttcctt
     6601 acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc gagttagcaa
     6661 aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat gtggtagatg
     6721 agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc actcatacga
     6781 taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt tatacgggag
     6841 attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca cgattggcgg
     6901 aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca gaaaatcctc
     6961 agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc aaatattggg
     7021 aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg
     7081 atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc gaacgaatta
     7141 ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg cttgttacgt
     7201 tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact ggccgaatgg
     7261 gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat gtcaagattg
     7321 aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa accatcgtgg
     7381 cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc tctgagaata
     7441 tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat ttgatgaaac
     7501 caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat gcagatttgg
     7561 ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga gacgtgctgg
     7621 aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat gtaatgattg
     7681 atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag
     7741 aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa
     7801 aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag
     7861 aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat gattttgagt
     7921 ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt gaacaaacaa
     7981 aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga aataaaaaat
     8041 aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt tttttgcgaa
     8101 aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag aataaaggga
     8161 gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa aagatcagct
     8221 atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa ctacaaaaaa
     8281 gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg
     8341 tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg agacaagtga
     8401 cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat acaggatatc
     8461 cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat tgttttttag
     8521 cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa atcgggagaa
     8581 gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac gctgatttaa
     8641 ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt gagattatat
     8701 ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac atactgtact
     8761 atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc aagtgatgaa
     8821 atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga aaattacgta
     8881 tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga tgattgaccc
     8941 gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga cagattggtt
     9001 gctgattact catggtcata gtgaccacat aggagatatg attcctattg caaaagaaaa
     9061 tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca gaggagtaga
     9121 ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt
     9181 acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata tgggcgagcc
     9241 tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg atactgctta
     9301 tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt tcttgccgat
     9361 tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc
     9421 caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag atcccgaatc
     9481 ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg aaacgctgga
     9541 agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt gaaagtttac
     9601 ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa
     9661 cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca attcaacgag
     9721 tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg acatttggcg
     9781 aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta gacaaggtct
     9841 tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat attacgccag
     9901 gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg gaagatggcg
     9961 ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag ctagctgatg
    10021 atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca accatgatca
    10081 accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt gatatctata
    10141 tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat tatcagcgtt
    10201 tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg
    10261 ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc gatcgggtga
    10321 tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa
    10381 tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca ttggaaggct
    10441 tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg
    10501 cagatacgat ttcggatcag atatctggaa atatccatac gattgatact gaccgtgaag
    10561 gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg gtcaatggcg
    10621 ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa
    10681 tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat tatttgcggc
    10741 tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc ggcagacgtt
    10801 ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg
    10861 tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac agcaagaaat
    10921 agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc gccctgatcc
    10981 tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt tcccagaaaa
    11041 agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag aaatgcaaga
    11101 agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag ctaattcacc
    11161 cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg accatcatcc
    11221 caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta gctgtagtga
    11281 gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc
    11341 acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc cctccacgac
    11401 gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg caagtgcttt
    11461 gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt atctttatca
    11521 aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag aaattcttga
    11581 taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg gagtgattga
    11641 cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc gcgtacgttt
    11701 acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag gaggccatcc
    11761 attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct ataaagagat
    11821 ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg
    11881 tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt ccgttacaat
    11941 agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg cccagaatgt
    12001 ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg cgggaacgaa
    12061 tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt agatgcaaat
    12121 ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa agtaaaaggt
    12181 gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat
    12241 cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc ggaatttgtg
    12301 aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac taatgataaa
    12361 ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta tggtttttta
    12421 gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc gtcagcgtac
    12481 gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt gactggtaca
    12541 caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt tcaacaaatt
    12601 gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt gtctcaagca
    12661 actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg
    12721 gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt tccagcagct
    12781 aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct tagctggaac
    12841 tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt ccaattggtg
    12901 acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga
    12961 ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc agcactgact
    13021 tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt attgttcggc
    13081 ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc tgagctaata
    13141 ccgagagtta gctgattgaa taccatttat ttggaataag aggatgtgac aagaattttg
    13201 tcacatcctc tttgttttgt tctcataacc ttttttcata tacagttcat gaaccgcttt
    13261 tattaacttg ccaaataaca ctgtattgtg cgttgtatcc agatagaaag tctccgtttc
    13321 atctgaaatt ttctcatgct gatctgtaat gatcaaatca gcatgttctg aatatgcggt
    13381 aattttaaca gcatctttgc gaaataactg ctgcaaatgg ctttgaagat gataatttcc
    13441 tgtaaactgt ttggttatct gcaaatggat gagtaactgc tctttcgttt ctgtttgtag
    13501 taagtgttgg atcaattgac taaggtattg actgatttgg tttctttccc cattttttga
    13561 aaaaattgta tcgatttttt ctcttaattt cttttcctgt ataaaagaac tagaatgatt
    13621 ggacgtggct tctggaaagc aaagtgtaga aaaactactg ctggattcac ccaacagttc
    13681 gaaaaaaaca actgccaaca caagaaaata aaaataaatt ggtattttat tttctaaatg
    13741 gttgagagga aactgttgag ttaagtaagt catcaattga ccgacttgtc gacaaataag
    13801 gtttttgctt gtggaaaata aaaaaccaat ctcctgtttt tgtcgaaaag agatttgatt
    13861 tggaaaaaaa caatagaaaa aataattaaa gtaggctctc tctgtttctg tctcttcaca
    13921 agggcgactc actgaaaaag aagtaaagta ttctgaataa tcatgtgtat gctgcaataa
    13981 gtccatcaat tcagtgattt cagaagaagt ttttggaaaa tatgcctgtt gtttgtttcg
    14041 taatatgaat aaagttaaca gcaagtagat atttttttct tgttttgaaa ttttttctat
    14101 ttgctcatct gtactgttca tttttagtgc atttaattca agtgaggaaa atggccaatc
    14161 gttacctcca aatgtatctg aaagaaaaaa gtaaagatag agacggatag ataactcatt
    14221 tccttcaaga gtacagcaat tttttacaga taggatccgt aaatggaatt ttattaagga
    14281 atgattgagc tgttttgtca gtttttcgag ataggagggc gatatataaa gttgttcaag
    14341 taaatatttt tttggtacag cagtttgtgg ataattgatt agtaacatca aatagttaaa
    14401 acgattggat tcttttaggt agcataattt taacttagaa aaaacagcta actttttagt
    14461 ttgatcggtt tctttggttt ggatctctcc tttatgatta gaataaataa attcatcaat
    14521 caataaaaat tgcttgagtg atacatttat ttttcggatc agttctttga ttgtccgttt
    14581 tggaatatgt aagtcattgg ataattcttg taaagaacaa ttattttttt gaatgaccat
    14641 ttgtagaaca agtaactctt tttttcttgc ttcaattagc ataaatacct cctttttatt
    14701 ctgtttttgt atatagttag acaaaaactg tgagtggaaa gtcatttcag tctgtgttat
    14761 tctcacagag tgtcgccttc ttttttatat aacgttattt aatttttttg ttaccttgtt
    14821 tattctattg tttatttcct agggtatttt gtcaagatat tcttgttttt aaggcggaaa
    14881 aattgatttt taatggatat aactaaatat gttttgataa ttatcaataa tatttttaga
    14941 attatttatg ttatttattt atgggtagat gaaaagagac ggaggtaaga atgatgggga
    15001 gagtaacaaa ttatagtaaa gaatatttgc tgttcaaaag tatggtatac gtggaagaat
    15061 atggaatgaa gtcaattact gcaagagagt tagcagagtt ctgcggttgt tctacatacc
    15121 cgatttatac acatttcaaa tcaatcagcg ggttaaaaga gaagatatta gaagaaatca
    15181 ctgtttattt tgaaaactac ttagctgaat gcagttccga tgatgtttta agcattgtct
    15241 acttattgaa agattttttt tctatccatg aaagtattcg tgaaatcatt gcaaaatctg
    15301 atttagacat ggattcgcta tttaagcggc catttttctc atatgtcaaa ggtcacacga
    15361 agatcaaaga agaaaagtcg cttgaattgg tatggcttaa tgcattggga agtttgcgct
    15421 cagatgattc ttctcaagat tttcttgaaa tgatgaacgc tgtttgggat aaaaacgagg
    15481 tgacggatga atacttggaa aatatcttaa aggaacagtc accttaggca gcaacatgaa
    15541 aaagtagatc agccaccttt atttttcgta aaaaagattg taaaatgtct aacgaaacat
    15601 tggaatgaag gaggaagcga caacgttacc acagcaattt atcaaaaaat atcgtcttct
    15661 tttaggcgaa gaagcatccg attttttttc tgcgcttgaa caaggaagcg tgaaaaaagg
    15721 atttcggtgg aatccactaa aaccggcagg attggacatg gtccaaactt accatagcga
    15781 agaattacaa cccgctccct acagcaatga gggatttctt ggcactgtca atggaaagtc
    15841 tttcctccat caagctgggt atgaatatag ccaagaacca agtgcaatga tcgttggaac
    15901 agctgctgca gcaaaacccg gtgaaaaagt tttggatttg tgtgcggctc ccggtggtaa
    15961 atcaacccaa ttagctgctc aaatgaaggg gaaagggtta ttagtgacga atgagatctt
    16021 tccgaaacgt gcgaagattt tatcagaaaa tatagaacga tggggagttt cgaacgcaat
    16081 agtgacaaat catgcacccg cagaacttgt ccctcatttt tcgggctttt ttgatcggat
    16141 cgtggttgat gctccttgtt caggagaagg aatgttccga aaagatccaa atgcaataaa
    16201 agagtggaca gaagaatctc cgctatactg tcaaaaacgt cagcaagaga ttttgtcttc
    16261 agcaataaaa atgctaaaaa ataaaggaca actgatttat tcgacttgta cgtttgcacc
    16321 agaagaaaat gaagagatca ttagctggct tgtagaaaat tatccagtaa cgattgagga
    16381 gattcctttg actcaatccg ttagttcagg acgttctgaa tggggaagcg tagctggttt
    16441 ggaaaaaaca atacgtatat ggcctcataa agatcagggt gaaggacatt ttgttgcaaa
    16501 gctgactttt catggacaaa atcagatgca taaagaaaag aagacacgaa aaaaaagtaa
    16561 agtgcaaatg acaaaagagc aggaaaaact ctggacggag ttttcaaatg attttcatta
    16621 tgaagctact ggaagacttt tagtgttcaa cgaccatctt tgggaagttc ctgagttagc
    16681 cccttctctc gatggtttaa aagtcgtacg tacaggtttg catttaggag atttcaaaaa
    16741 gaatcgattt gaacctagct atgcattagc gcttgctaca aaaaagatag agaatatacc
    16801 ttgcttgccg atcactcaaa aggaatggca atcttacaca gcaggagaaa catttcaacg
    16861 tgatgggaat caaggatggg ttttgctagt attagataag atacctgtcg gatttgggaa
    16921 acaagtaaaa ggaacagtaa agaacttttt ccctaaagga ttgagatttc actgagtgga
    16981 ttagttcgtt tatttttttg tctatgctat gatggagata gataaaaagg agctgatact
    17041 tatgaaaaca tcaacaaaag taacaatcgg attaagcgta gcagccgctg ccagtgtagc
    17101 gacagcagtc gttgtatcgg gaaaagtaat ggaaaagatc catcatatga ctactcggac
    17161 gaaagtaaaa aaattcgtct atgacaaatt tgatggaaat gaaaaattac tagaaatcgt
    17221 agatggtctt tcagatagtg acttggattc tttgatggga atgttagcaa aaatcaaatc
    17281 aggaaaaaag aaaatctctg tttatggtga ttcaatcaaa gattcaaccg aagatgtaaa
    17341 agaccggcta atgcattttg ttgaaaaaat gatgtaagag aaactagctt ttgaacacta
    17401 ttcattcgaa ataaggggtt gtgacaaaat tattgtcaca acccctttaa tctatcttta
    17461 attatgttca tgaagcaata aaagatagca aaacagtaaa tcgttggctt tttttagatt
    17521 gaaaccagac tgttcttgaa atttttcgat tcgatatttt agagtattac gatgaagaaa
    17581 cagtgttttt gcagcagagc tgacattgcc taattcattc cataattctt gaatgatcac
    17641 ttccatttca ttttgtttga tcagttctat gtaacttttg atcaacttac tatcttttac
    17701 agtatctttt gtatagtaat gtaaagccac atcttgcata gaaaaagtac ggctgtgagt
    17761 ataaagattg ttttgctctg ttaaaaaaat ttttctttct tcagcaaaca aagggacgag
    17821 gtcggtgcca ggtgaatgga aagaaccaac gaatacagac gttgtcgtat caaaatcagc
    17881 atctaaagat agaaaaatac cgttgatttc agtagcatca agataatcac ggtttatttg
    17941 ttcgactaag agcgcatctg tgtctgtata aaaaaagaaa tcagcagaat agcgaaataa
    18001 ttccaaaata gtatcttgcc attctttttt taagaattct ccttttgatt cgacgtgaag
    18061 ctggatcaaa cgaaagcttt tttcttgaac acaagaagca tctttaaaaa gatagtgata
    18121 ccatgggtgt tttttttgat cgttgattac agggaataac gctttcaaaa ggctggtttc
    18181 ttgttgagat aatgaatctt gtttgatcca gataaaagca tcatcaattg caaaagaaag
    18241 gatatccgct tctgatgctg gagagttttt tttctgtgct tgtgggtaaa gagcaagtaa
    18301 tttatccatt ttcatcttgt tcacctcttg tatttatttt accatagaaa aaaagttagt
    18361 tacagccttt ttatcatatg ctaaagtaaa acaagaaaaa acaaaggcag ggaagggtaa
    18421 ttggtaaata caaatcttga tgcagtagaa aaagaaaaat ggatgcgcct tgcattggca
    18481 gaagcaaaga aagcagaaat gcttcatgaa gttccgattg gagcagtggt tgtattggat
    18541 ggaaaagtga ttggaagagg atataacctt agagaaacca cgcaagatgc tactacacat
    18601 gcagaaatgc ttgcaatcaa ggaagcctgt gaaaaagtgg gaagctggcg cttggaagat
    18661 gctgcacttt ttgttacctt agagccttgt cctatgtgca gcggcgcgat gattttgtct
    18721 cgtgtagcag aagtttattt cggcgcgtat gatccaaaag gcggaacagc cggtaccttg
    18781 atgaatcttt tggaggacga gcggtttaac caccaagcat atgtagaagg tggcatttta
    18841 gaagacgaat gtgggatgat tttgactgac tttttccgaa agctccgcca gcgaaaaaag
    18901 aaattaaaaa aagactggtc aacgaatgaa aaataaggta tactattctt tgccgaaagg
    18961 ctaggacaat ggtgggcttg tgaagcgtgt caggtctgga aggaagcagc actaagcagg
    19021 ttccgccatg tgtctgatta taatgaaacg acaacagact ttcgggtctg ttttttttat
    19081 ttttatagta ctgtaaaaat gaatgttcct gattttttag ccagactgat ttatcgaata
    19141 ttgtacttat acaatttgct tcaaggcagg aaaaactctg aaatagagct tgtttcaatg
    19201 gaagaaaaag ctgcctatgg tatgataaag ataaagagag gcgagagggt acatggaaaa
    19261 aatagtgatt ttacatacga atgatctgca ttctcattta gaaaattggc caaggatcag
    19321 aaggtttttg gatcaacgta aacgagaaaa cgaaaagaaa gaaaaaacga caacgattgc
    19381 agtagatcta ggtgattttg tggatcgttg gcatccattg tcagaagcaa cgaatggaca
    19441 ggaaaatatc ttgctgatga atgaagttgg ttatgatgca gcgacgatag gaaacaatga
    19501 aggcgtagga aattccaaag aagagttgaa tcatttatac gatcatgctg agtttgatat
    19561 catcctggat aacttattcg ataaaaatac gctccagcca ccggcatgga cgaaaccata
    19621 taaaatcatt acaacgaaac accagacgaa aatcggacta attgcattta ctgctccatt
    19681 tccgcttact tataatccga atggctggga catacggaat ccttatgata ttttgccaga
    19741 attagtcgaa gatttacgtt cacaagtgga tgttttagtt ttgatgagtc atctcgggat
    19801 tcaggatgat ttccaaattg caaaagaaat accagcaatt gatgttattt taggatcgca
    19861 cacccatcat ttattcagag aaggtcacgt aatcaatgat gtccaaatag cagccgctgg
    19921 aaagtacggg cagtatgtcg gtgaagtcca tttaaccatc gatgaagaga aaaaaatcct
    19981 taagcattca gccaaagcca tccctaccga aacaatgaca gcttttacag aagatgaaca
    20041 agaaataaat agttgtctag aacgtgggca tgaattatta aaagaaaaag aagtagcaga
    20101 tttgccattt tctctgagtc tagatatctt caatgagcat tcgtttatcc aagtagcctt
    20161 tgaggcggtg aaagaacgag gaaagacgga agcagcaata ttaaacagtg gtctgttttt
    20221 aactgaaatt ccaaaagggc gggtcaatca ggatcagctt cacactgcgt tgccgcatcc
    20281 gatgcacttg ttaaatgtca cattgggtgg aaatgatctg atccgcttag ttttagaaat
    20341 agaaaaaaat cgtaactttt tacgtaacta tcctatgaag ggaatgggat ttcgcggtaa
    20401 gatttttggg cagatggtct ataatggaat cacatatgat tctattaacc atcaagtctt
    20461 ttggaagaac aagccaatcg atctgaataa aaattatact tttactaccg tagatcattt
    20521 gatgtttgtt ccattttttc caacgattga gattgctgga aaaaatgaat ttttgttccc
    20581 tgaatttatc cgaagtgtgg taggagatta cttgaaagct cactacccaa tcaaataaaa
    20641 acaaggtata ataaaaaaga taggtggtga acgcatgtct gcaaaacaaa atgaacagca
    20701 gaacgttgag cataaaacag aaattgctaa gaaaaaatca tcagctgaac aggaaacaac
    20761 agtcaccgaa gaactgacag ctgctttgga agaaatcgtt gaggagccta aagcagaaaa
    20821 agtcaaaggc gagctagtga cactgttaga tgacagtaat tttttgatag gtgaaagaca
    20881 ttatcggctt gtgtccgatt atcgtgaagg atttaatgca gaaaagcttg gagaacgtta
    20941 cagtgatgta ctagcccgat atgattatat cgtaggagat tggggttatg aacaactacg
    21001 cctaaaagga tttttccaag cggataatcg acgggcgcat ccagatcagc gaatcgattc
    21061 cttggaagac tatctttacg aatattgcaa ttttggttgt gcgtattttg tcatcgagag
    21121 aatcggcggt aaaaaagaaa agacaactca gcgccgaaaa aagaaaaaaa accataatcg
    21181 tgcgtatatc gatgagaaaa aaggaccagt cgcatctaat cgcaaaaaac cggttattaa
    21241 aaatcgaaaa acagagtcag caaaaacctc tgaaaaagta aaaaaacaag aacaacctaa
    21301 acaagtagaa aaaacaaaaa cagcatttac gatcagaaaa agggaagagt agtggatgaa
    21361 tggactataa aggatattta attgatttag acggaacgat ttatcgtggg acacaaccca
    21421 taccggccgg taaacgattt gtggaagaat tgcaaaaaag aaaattacct ttcttgtttg
    21481 ttaccaataa cacgactaag tcaccagaaa cagtagctaa tcgtctagca gatgagtttg
    21541 acattcatgt agctccagag acagtctaca cagctacttt ggcaacaatc gattttatga
    21601 aagcagatgg aaaagggaaa aaagtctatg ttatcggaga agcaggattg attgacttga
    21661 ttttagctgc cggatttaca tgggaagaag aggctcctga ctatgtagtg gtaggcttag
    21721 ataattatct gacctatgaa aaagtagtta aagcaacact tgctattcaa aaaggtgcta
    21781 ctttcattgg aacgaaccca gataagaata tacctactga acgcggcctt cttcctggag
    21841 cagggtcagt catctctttt gtagagacag caacccaaac gccgccgatc tacatcggaa
    21901 aaccagaagc catcattatg gataaagccg ttgaagtatt agggctgcaa aaagaagaag
    21961 taatcatggt tggagataac tatgaaacag atatccaagc tgggatacgc aataatatag
    22021 atactttgct cgttttatca ggttttacaa aagaagaaga tgtacctggg ttgcctatcc
    22081 cagctaccta tgtgaaacaa tcactggatg aatggagctt ctaatatgaa aaattcgaga
    22141 tggcagtgga tggaatatgc tggattgttc agcctatttc tgacactgat cagtctggca
    22201 gttggtgtga cgatcaactt tcgaccacta tacgtatttg atatcggaca tttgcaaatt
    22261 ttggattata cttctttaga tcaagagacg ttactgaaaa attttgatca cctaatgaat
    22321 tatctcaata atcccttcaa aaccatcctc agcttgccag atttcccagt ttctgctagc
    22381 ggagcccatc acttttatga agtgaaaatt ttgtttttag tggattatgc tgtgtttttc
    22441 atcactttga tcccgagtat tttgtttatc aaatatttgc aaaagaatga tcggctttgg
    22501 cggttgattc gtccgtttca aatcggcatg ttactgccag ttgttttcgg ttttttcatg
    22561 atgataggat ttgatcgctt ttttatcctt tttcatgaaa cgtttttcaa caacgatgac
    22621 tggttattcg atcccgttac tgatccaatc atcaatgtac ttccagaaga atttttcatg
    22681 catagcttta tcttattttt tgtattatta gaattatttt ttgctgtttt tcttttctta
    22741 ggaaaaaata gcttgaaaca aacaaaaaag aaagaactcg tctaaataga cgttttcttt
    22801 ctttttttgt tgaattattg agtaaggcga cttctttttt ctggttcttt gtgtgctaat
    22861 cgactggcag cagcagcggc gatcgctccg acgatatcat ctaagaaggt atggacatga
    22921 acgccatcgt ggctgtttag ttcttttaaa ataccaggtt tcaccttgtc gatataacca
    22981 taattcgtaa agccgatcgt accgtaaaca ttcacgattg acaaggctag gatctcatcg
    23041 ataccgtaaa ggccttcgtc atcttcgata atatgttgca acggactcat caattttttc
    23101 ttttctgcta atatatctaa ttggattcca gtgataatgg cgttatgtac ttcacgcttt
    23161 gttagcacgc tgttgacact ttcagtacaa tccgccaaag tcaaatcaga aatatagtct
    23221 ttttgtaaaa acatgacaag ttgtgcgata tcttctacag ttacgccacg ttccttcaat
    23281 agttctcttg ccttttcttc taaagtctca gttttgacga ccatattatt gctccccctt
    23341 tattttttct ttctctattt catgttaacg gaacatgaaa aaaatgcaag aaactgcttt
    23401 cgggtttaac ttgaaatgca aagagatata aaaaatcaac taggacgaag atttttatcg
    23461 tataagcttg tgctgtgacc aggctgagtt cgttcctttg gattaatata gtgtaaagcg
    23521 ttgttcactg cagtaggtgc ttcaccgagc ccagtagcaa tcagtttcac ttttccttca
    23581 taggtacaga tgtcaccagc tgcataaacg cctggaatag aagtagacat atctgatttt
    23641 acagtaatgg catttctggt actatcaagt ccccaagaag aaagatgttc taaattagaa
    23701 gagaatccat aattgacgat cagagaatca atcatcaaat caatcgtctc gtcacttttc
    23761 acttttttta accggatatc tgtcagttcc ccattattgc ctgataatcc atcgatgaga
    23821 tagggagtaa gcaagttgac agatgaagat ttcaagcgtg atacactatg ttcgtgcgca
    23881 cgaaattccg ggcgacgatg aatcaagtaa acttcgcttg caataggctc taacatcaac
    23941 gtccaatcga tagcagaatc accacctcca gcaatagcga ctttttttcc agcatacttc
    24001 atcaaatcat tcacgaaata atccaatccg tggttttcaa atgattcagc attatctaaa
    24061 ttcaattttc ttggctggaa tgaaccattt cctaaagcta aaatgactgc tttggaataa
    24121 tggatgcctt tatttgtagt aatctcgata acttcgtctt cacgagtaag cgagagaact
    24181 tcttctttta aatggaatgt atggttaaaa gtagttaatt gtttttctaa ttgatcgatt
    24241 aattcacttg cttttattgc tggataccca ggaatatcgt aaatatattt ttcgggatac
    24301 aaagttgcta attgaccacc aagttgaggt agactgtcaa tgatttttgt tttagcttgg
    24361 cgcattcctg cgtaaaaagc ggcaaacatc cctacaggac ctgcaccgat gattgtaatg
    24421 tcatagattt ccataaaaat tcctcccttt tcacactata tcagaattta attgagaatg
    24481 cttctcaaaa atgtttgaat tcgaaatcga aacggtaagt tatagaatgt atctagtgat
    24541 gaaaaataaa agaaaacaaa tctagtattt ttacgagagg ttaggtatta tgttattatt
    24601 attatgaagt atgcaaatat taggaggaaa caatatggca ttcccacaac tagatttaaa
    24661 aagtgaaaaa ggaccaaaag ctgttatcaa aacaaaccgt ggtgacatta ctgttcaatt
    24721 attccctgaa ctagcaccga agacggttca aaatttcatt gaactttcca aaaaaggtta
    24781 ttatgatggc gtgatttttc accgcgttat tcctgatttc atgatccaag gcggcgatcc
    24841 aactggaaca ggaatgggtg gcgaaagtat ttatggcgaa agttttgaag atgaattcag
    24901 tcgtgaactg ttcaaccttc gcggagcttt atctatggca aattcaggtc caaacacaaa
    24961 cggaagccaa ttctttattg taaataacac aaatgtgcca gctaatatgc ttggacaact
    25021 agaaggtgct ggattcccat cagaaatcat tgaagcgtat aaaggtggcg gaactccttg
    25081 gctagatttc cgacatacag tctttggaca tgtactagaa gggatggaca cagtagatga
    25141 aatcgcaaat gtacaacgtg gtccacaaga ccgacctgta catgatgtag tgattgaaac
    25201 aattgaaatt tcagaatagt ttggtatggt acggagtaat tatgaaaaag gacgaaacct
    25261 agtaaggtct cgtcctttta ttttgaataa atgatttaat cgattgtctc taaagcatac
    25321 ttgattcgat taagtccttc cagtaaagtt tcttctggac aagcaatatt caagcgcata
    25381 tgcccatgac cggaaggacc aaacgtgatg ccaggattta atacgacttt tcctttccga
    25441 acaagagtgt tctccaattc ttggtcgtct gaaatatagg cggtaaaatc taaccacatt
    25501 aagtaagttc cttcagggtt cattattctc actttcggaa gatactgttc aaaaaactga
    25561 ttaactgttt gaacattttt ttctagataa ggaatcagtt ggcttaacca ttctccaccc
    25621 gtctcataag ccgcttgggt tccaatcagt ccaaaagtgt tgatttcgtg ctgttggttc
    25681 atcactaaat gtttttcaaa atcagctttc atctctggat ttttgataaa taccattgaa
    25741 tttttgattg cagctagatt gaaagtcttg gttgcggaag taaaagcaac tagtagatca
    25801 tctaaattag gatcaatcgt tagcatggaa gtaaatgtat gatcgaataa agttaaatct
    25861 tgatggatct cgtcactaaa cagaatcact tgatgtttta aacacaattc gcttaactgt
    25921 ttcaattctt ctttgctcca aacacgccct ccaggattgt gaggattgca taaaatcatt
    25981 gcttttacat tgttttcttc gatttttttc tccatgtcag acaagtccat gatgaaatga
    26041 ttattttttt ctagcaaaga gcttcgaacg atgtgacgtt gatttgtttc tataatagag
    26101 gaaaatggag gataaactgg gtcatgaatc aaaatagaat cgcctggttt tgtaaaagtt
    26161 tgtactgctg cagctaaact ggttaacaca ccacttgtaa aaacgatatt ttctttcgtc
    26221 aactccacag catggtgttg gcgttcccaa tgaatgatag cctgatatag tttatcagac
    26281 agagtggcat aaccaaagat tccatgtttg atgtattcac taaacgcttt agctacccca
    26341 ccgggagaca agaagtccat atctgctacc cacaaaggaa gtaagtcatc catttgatat
    26401 gtctcagcaa ttgcgtccca ttttacactt tctgtatctt ttcttgaaat ctgtttatca
    26461 aattcgatca tcttaagacc tccagtcatt ttcgtcgctt ctttttttga gctatttaca
    26521 gtataatgat aaatgcgaag agagggaagt agatgactta taaaattgga caaataattg
    26581 aaggaaaagt gacagggata cagccttatg gcgcattcgt ttctcttgat aacgagacgc
    26641 aaggacttat tcatgtttca gaagtacaat ccggttatac gaaaaatatc cattcattgt
    26701 taaaagtcgg acagccagta acagttcaga tcatcgacat tgatgaatat tccaaaaaaa
    26761 tcagtttatc tttaagaacg ttagaaaaaa caaatccagc tgttccatat cgcagaaaaa
    26821 ggtatttcac gaataaaaat cgtaagatcg gatttaccac tattgcggag cagttaccta
    26881 tttggataga agaagcatta tctgaactag aacggaaaga aaataagaaa taaaaagtgt
    26941 attttttctg aaaatgtgct agaattttta tgtaagccat agaacaagtt gatcgaggtg
    27001 gaaattttgg caagcaaaag agttgctggt acaattatgt tgcatttgga ggatggtact
    27061 aaaaaatttt tggtccattc aattgatgac aagttggaat ttgcgttagc ggaactttct
    27121 gaaggaaaga ctggtttagc gaatatactt aatttcttaa aagaaatagt tcatatcgat
    27181 gttagcaaga ttagtttgat ggaattgaca aatacacata ttaaagagga aaatgttccg
    27241 ttgtttgtct ttgaaactga acaaaagaat caaagagaag aattaggcga aggatatatt
    27301 tgggaagaac caggtcaaat gcgctctgtt cttggaactt acgaagtaga gggagtgcct
    27361 ttcttctaaa atacagatta agcgaacaaa gaagtttgaa tttttttcaa acttctttt
//
LOCUS       DAYYAG010000028        26935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000028 DAYYAG010000000
VERSION     DAYYAG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.028"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1116
                     /locus_tag="ACHARQ_001024"
     CDS             112..1116
                     /locus_tag="ACHARQ_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409462.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1117..2061
                     /locus_tag="ACHARQ_001025"
     CDS             1117..2061
                     /locus_tag="ACHARQ_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409463.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2061..3023
                     /gene="opp4B"
                     /locus_tag="ACHARQ_001026"
     CDS             2061..3023
                     /gene="opp4B"
                     /locus_tag="ACHARQ_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8409464.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3050..3964
                     /locus_tag="ACHARQ_001027"
     CDS             3050..3964
                     /locus_tag="ACHARQ_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409465.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3990..5771
                     /locus_tag="ACHARQ_001028"
     CDS             3990..5771
                     /locus_tag="ACHARQ_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8409466.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6119..6805
                     /gene="rnc"
                     /locus_tag="ACHARQ_001029"
     CDS             6119..6805
                     /gene="rnc"
                     /locus_tag="ACHARQ_001029"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8409467.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6825..10406
                     /gene="smc"
                     /locus_tag="ACHARQ_001030"
     CDS             6825..10406
                     /gene="smc"
                     /locus_tag="ACHARQ_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8409468.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10415..11224
                     /locus_tag="ACHARQ_001031"
     CDS             10415..11224
                     /locus_tag="ACHARQ_001031"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8409469.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11235..12233
                     /gene="ftsY"
                     /locus_tag="ACHARQ_001032"
     CDS             11235..12233
                     /gene="ftsY"
                     /locus_tag="ACHARQ_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8409470.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12420..14687
                     /gene="gshAB"
                     /locus_tag="ACHARQ_001033"
     CDS             12420..14687
                     /gene="gshAB"
                     /locus_tag="ACHARQ_001033"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8409471.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14776..15228)
                     /locus_tag="ACHARQ_001034"
     CDS             complement(14776..15228)
                     /locus_tag="ACHARQ_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8409472.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15378..16328
                     /locus_tag="ACHARQ_001035"
     CDS             15378..16328
                     /locus_tag="ACHARQ_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8409473.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16321..17280
                     /locus_tag="ACHARQ_001036"
     CDS             16321..17280
                     /locus_tag="ACHARQ_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8409474.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17277..18032
                     /locus_tag="ACHARQ_001037"
     CDS             17277..18032
                     /locus_tag="ACHARQ_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409475.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18055..19008
                     /locus_tag="ACHARQ_001038"
     CDS             18055..19008
                     /locus_tag="ACHARQ_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8409476.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19652..19948)
                     /locus_tag="ACHARQ_001039"
     CDS             complement(19652..19948)
                     /locus_tag="ACHARQ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8409477.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20303..20659
                     /locus_tag="ACHARQ_001040"
     CDS             20303..20659
                     /locus_tag="ACHARQ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8409478.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20669..21568
                     /locus_tag="ACHARQ_001041"
     CDS             20669..21568
                     /locus_tag="ACHARQ_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8409479.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22088..22816
                     /locus_tag="ACHARQ_001042"
     CDS             22088..22816
                     /locus_tag="ACHARQ_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8409480.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKSVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22961..24988
                     /locus_tag="ACHARQ_001043"
     CDS             22961..24988
                     /locus_tag="ACHARQ_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8409481.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25096..26193
                     /locus_tag="ACHARQ_001044"
     CDS             25096..26193
                     /locus_tag="ACHARQ_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8409482.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26500..>26935
                     /locus_tag="ACHARQ_001045"
     CDS             26500..>26935
                     /locus_tag="ACHARQ_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409483.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT         9031 a         4665 c         5684 g         7555 t
ORIGIN      
        1 aattcattac acaaagataa gaataacctt tgttataaaa cgctttattc tgtgtaatgt
       61 ccttttttat taagaaaaac ttccatttag gaagcagaga ggggtattcg attgtccgat
      121 aaccaattat tagaagttca gaacctacat acaggattcc gactcaaaga tgactattac
      181 gatgcagtag atgacgtttc atttacttta gacaaaaacg agattttagc aatcgttggg
      241 gaatctgggt gcgggaaaag taccttggct actacgatta tgggattact tgatcctaat
      301 aacacaaaaa ttacaggtga gattttatat aacgatctga atttaatcga cctgaacgaa
      361 acgctctata ataaaatccg cggaaatgat atcggaatga ttttccaaga tccgttgtct
      421 gcattaaatc ctctcatgag aatcgaagac cagatcaaag aaggactttc ctatcacaca
      481 aagatgaatg cagaacagcg tcaagctcgt gcactagaat tgctagaaca agtagggatt
      541 cctaatcctg cgagagttgg acgccaatat ccccatgaac tatcaggcgg tatgagacaa
      601 cgtgtcatca tcgcaatcgc aatcgcctgc aaacctccaa tcgtcattgc agatgaaccg
      661 acgacagccc tagatgtaac catccaagca cagattcttg atttactgaa agaccttcag
      721 gaagaaacaa aaacagggat catcttgatc actcatgact taggagtcgt agcagaaatg
      781 gctgatcgag tggcagtgat gtatggcgga cagttcgttg aagtagcagg tgtaaaagaa
      841 ctgtttgatc atcctaagca tccttataca cgctctttac tgaactctat accacaagaa
      901 ggaaatcatg atgcagaact ccatgtgatc gaaggtgtcg ttccttctct gaaaaacatg
      961 cctcgtaaag gttgtcggtt tgcaccacgt attccatgga taccagcaag cgatcatgaa
     1021 gaagagccta tccttcatga agtcgcaccg aaccacttgg tacgttgctc ctgctataag
     1081 catttccact ttagagacga aaaaggagag gtgtagatga cagaattaat cacaatcaaa
     1141 gacctgaaag ttcattaccc tattcgcagt ggattcttca atcgagtaac ggatcatgta
     1201 tatgctgtag atggtgtgga ttttattatc gaaaaaggaa agacatatgg tttagtggga
     1261 gagtctggct ctggaaaatc tactacggga aaagccgttg tgggattaga aaaggtaaca
     1321 gccggacaaa tcatgtatga aggaaaagat gtaacgaagc gaagcaaccg caaaaaaatg
     1381 ggttataaca aggatgtgca gatgatcttc caagattcaa tgtccagttt gaatcctaaa
     1441 aagcgagtgt tggatatcat tgctgaaccc atccgaaatt ttgaacggtt aagcgatcaa
     1501 gaagaaaaga aaaaggtaaa aggactgcta gatatcgttg ggatgccgga agatgcgcta
     1561 tataaatatc ctcatgaatt ttcaggcgga caacgtcaac gtctaggagt agcccgtgct
     1621 gttgctacca atccaaaact gatcgtagcc gatgaacctg tatctgctct ggatctatct
     1681 gttcaagcgc aagtattgaa tttcatgaaa cgaatccagc aagaatacgg cctcagctat
     1741 ttattcattt cacatgattt aggtgtagta aaacacatgt gcgacaatat cgcaattatg
     1801 tacaaaggtc gatttgtaga aatcggtaca agagaagaca tttataatga cccgcgacat
     1861 atctatacga aacgtctgct ttcagctatt ccgcaaatcg atgtagaaaa tagggaaagc
     1921 cacaaagaaa atcgacgaag agtagaacaa gagtatatcc aaaaccaaaa agaatactat
     1981 gatgcgactg gtcgagtcta cgatttgcgt acgatcacaa ctacacacaa agtggcgttg
     2041 aaagatggag gtgcaagcta atgtggaaaa cgattctgcg acgtgtttta ttgatgatcc
     2101 cgcaagttat tattttaagt gttctgatct tcttattagc ccaagcgatg ccgggggatc
     2161 catttaccgg tttgatcaat ccaaatcagg atccggcagt gatcgaacag atgcgacaag
     2221 cagctggctt gaatgaccca tggtatgtcc agtacttccg ttggatcgga aatgctttgc
     2281 aaggcgattt tggtcaaagt ttcttataca aacgctctgt tgctgacttg atcggcgaac
     2341 ggatcatcaa cactttatat ctatcgatct tgacagttgt ccttacttat ttgattgctg
     2401 tgcctctggg gatgcttgct ggccggtatc aaaattcaat cttggataag attgtcgtaa
     2461 tctacaactt ctttagtttt gctgttcctt tatttatctt cggcttgatc atgctatttg
     2521 tttttggtta tcgtttaggc tggttcccaa caagtggatc acaaacacta ggtttgcaag
     2581 gtggatttat cactcaatgg atagaccgtt tgaaatatat tctgttacca tcaattaccc
     2641 aagcattttt ggcgactgcc gtcacaatcc aatatctgcg aagtgaagta atcgattcga
     2701 aatcgctcga ttttgtcaga acagcccgtt ccaaaggggt accgacgaat aaagtattca
     2761 gtcgacatat tttccgaaat gcagcgttgc cgatggcttc tcaaatgggg tatgaaatca
     2821 cttcgttgat tgctggttct gtagttatcg aaaagatttt tggttatccg ggagtcggaa
     2881 aactatttat tgattccatc ggccaacggg actataccgt gatcacatcc ttagtattga
     2941 ttttaggaat cgcgacgcta gtggggacat tactttcaga tatcatcatg agtatcgttg
     3001 acccgcgtat tcggattcaa taaaaccaat cagaaaggag aatggatcga tgagtgaaaa
     3061 aaacaataca gcagttcaag aaagtattcc tcctatgggg atacgaatga ttgctcgaga
     3121 atttaaaaaa gataaaattg ctattttttc attaggtttg ctagtggttt tattattagt
     3181 gatctttatc ggtgcgatgc tgaccgatca agacaaagtg atgtatgtta gtatcttcga
     3241 caaatacgct gcaccaggaa cagtttcgac acaaggacaa aagttcattc tgggagcaga
     3301 tgaaggcgga cgtgatgtat taggacaatt gatcataggt gctagaaact ctgttacgat
     3361 cggatttgcg atcacgatcc ttacttcgat catcggggtt ggattaggga tcatctctgg
     3421 ctactacgga gggatcattg acaacatctt gatgcggatc gttgatttta tcatgattct
     3481 accgatcatg ctgatcatta tcgtcttcgt atcgattatt tccagataca atgtctggtc
     3541 ctttatattc atcatgagtg cattttattg ggtagctaaa gcccgattat tccgaagcaa
     3601 gacattatct gaagcaagaa gagattatgt cagtgcctca aaaacaatgg gaaccaatga
     3661 ttttaaaatc atgttccgcg aaatcatgcc gaatctaagt tcattaatca ttactaattt
     3721 gacgatgaac tttgcggcaa acatcggtat cgagacaact ttgactttct taggtttcgg
     3781 cttgccggca aatgtaccta gtttaggtac attgatcggt tatgcaagta acggagaagt
     3841 cttagtgaat cggcaatgga tctggttacc cgcatcaatc cttattttag ttttgatgtt
     3901 gagtatcaat tatgttggac aagcattcaa gcgttctgca gatgcgcgcc aacggttagg
     3961 ctagtataga agcgaagggg gaaataggaa tgaagaaaac gatttttgga tttgcagcgc
     4021 tgatgtctgc tgtcgttctt gcagcatgcg ggggcggagg tacagatacc tctggaaaca
     4081 actctggagg caacggcaat aacaaagagg aattaacttt tccaattgca acaaaaaata
     4141 atgatgatgc aatcgaagac ggcgaattag aagctgcggt cgcaacggac agtcagttta
     4201 aaggattgtt ccagtgggag ttttaccaag attcatatga tgcagctttt atggctcctt
     4261 ctcatgaacc gctatttgca aacgatgacg atttcagaat cacagacgac ggagctgcaa
     4321 cgcttgatct agatgaagat acgaaaaaag cgacaatcaa agtaaaagac aacgtcaaat
     4381 ggtcagatgg tgaagatgta acggcggacg atgtgatttt cccttacgaa atcattggga
     4441 ataaagatta cactggtatc cgttatgacg acactttccg taacatcgta ggtatggaag
     4501 aatatcatga tggaagtgcc gacacaatct ccggaatcaa aaaaatagac gacaaaacag
     4561 ttgaaatcga atataaagaa gtaaacccaa gcatgcttca atcgggtggt ggtatctggt
     4621 catatgcagc accaaaacat acgttagaag gtgtagcaat caaagatatg gaatcaagcg
     4681 atcaagtacg taaaaatcca gtgacttttg gcccttatta catgagtaat atcgttgcag
     4741 gagaatcagt agaatacttg ccaaacgagc actactggaa tggtacgcct aaattgaaaa
     4801 agatcacgat gaaatctgta ccgatggcaa gtgcaactga agcattgaac tctaaattat
     4861 atgatatgat tttccaaatg ccaacagata cttacgatac ctataaagaa attgctggct
     4921 atacaaactt aggccgtgaa caaacttctt atacttatct aggattcaag ttaggtaaat
     4981 ggaatgcaga tacaaagagt gtggaataca atccagattc aaaaatggct gataaatcat
     5041 tacgtcaagc aatgggctat gcattagata ataatgcagt aggtgaacgt ttctatgcag
     5101 gtttgagaag caatgcgact tcattgattc ctccagtatt ccagtcatac catgatgatg
     5161 agatgaaagg attcacacaa gatctagaca aagcgaaaaa actattagat gatgctggct
     5221 acaaagatgt ggatgacgat ggtttaagag aagacaaaga tggaaataaa ctaacaatca
     5281 actttgcttc gatgtccggt ggggaaacag cgcaaccatt ggcagattac tatatccaac
     5341 aatggaaaga aattggtttg gatgtccaat acacaacagg tcgtctgatc gaattcaatt
     5401 ctttctatga taaattagaa aacgatgatc ctgaaattga tgtttaccaa gctgcttgga
     5461 atacaggaac tgatccaaat ccaagtggac tatggggacc aaatgctgcg ttcaactaca
     5521 ctcgttatga atcagaagaa aatacaaaat tgatcaacga tatcagttca agcaaagcat
     5581 ttgatgaaga gtaccaaaaa gaagcatatc aaaaatggca acaatatgca tttgaagaag
     5641 catttgcgat tccaacgttg ttccgtaatg aagtattgcc agtcaacgac cgtgtcaaag
     5701 actgggattg gtcttatgat gtaccaaacc catgggcaaa tgtttctgtt acagcagatt
     5761 cacgttcata atcactaaaa atgaccgaaa aagggcgatg tcctttttcg gttttttgat
     5821 atttttctgt ttatttctct aatattcagg tcaaggcgct gatttcatga cttctccaat
     5881 aaacggtgtt caaagtccag tttctcggta ttaagtcaaa tttatgcaaa aatatggaaa
     5941 gccattttca caaaattttt tcttcatact cgaaactaaa cgaatttttc acaacttctc
     6001 gtcaatcact aggattatta aagctttact taccttttga ccgaaccatt tcaaatttga
     6061 caaaaaacca gtataataag gaagtatcga aaaaatatcc ataaaaggaa tgtggaaaat
     6121 ggacaaccag ctgacaaaag aactaaaaga aaactataac atcgtttttc acgatctaaa
     6181 tttgcttgaa caagctttta cccattcatc ctatgtgaat gagcatcgca atctgcaatt
     6241 atcggataat gagcgacttg aatttttagg agatgccgtt ttagaactga tggtttctca
     6301 gtatttattc cgtttatatc ctcatttgcc agaaggaaaa ttgacaaaaa cacgagctgc
     6361 gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa tgtcactttg atcaatatgt
     6421 tttgttgggc aagggagaag agaattcagg aggacgtacg cgaccagcat tgctttgcga
     6481 cctattcgaa gcttttttag gcgcgttata tcttgaccag ggattcgaca cgacccatgc
     6541 attcatcgaa aaagtgattt ttcctaaagt caaagcaggt gctttttcac atgagatgga
     6601 tcataaaaca aaactacaag aagtcttgca aaaatcaggt gatgtatcaa tcgaataccg
     6661 attgatcaat gaagaaggtc cagctcatga acgagtattt tggatcgaag tttatgtaga
     6721 tgatcaattg attggcactg gacaagggaa atcgaaaaaa ttagcagaac aagctgcggc
     6781 ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa agctttgtat ttgaaacgaa
     6841 ttgaaatagc cggttttaaa tcatttgcag acaagacagt cattgatttt gaaaatagcg
     6901 tcacagcagt ggttggacca aatggcagcg gaaaaagcaa tattacagag gctatccgct
     6961 gggtattagg agaacaatct gctaaaagtc tgcgaggcgg aaaaatgccg gatatcatct
     7021 ttgccggttc cgatacccgt aagcagctga atattgcaga agtcacagtg attttagaca
     7081 atagtgatca ttatctgcca ttggagtaca atgagatcag tgtaacccgc cgttatcgac
     7141 ggactgggga aagcgagttt ttcatcaata aacaaagctg cagattaaaa gatatccaag
     7201 aattgttctt agattctgga ttaggaaaag aatcgttctc aatcatttct caaggaaaag
     7261 tagaagcgat tttcagtagt aaaccggaag accgtcgtgg gatctttgaa gaagcagctg
     7321 gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca gaagcttttt gaaacagaag
     7381 acaacctcag ccgcgtccaa gacatcatcc acgaattgga agaacagtta actcctttgg
     7441 ccgcccagag tgaggcagca aaggagtttt tacggctaaa agaaacactg actcaaaccg
     7501 atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa agattgggac aacaaacaag
     7561 cacagcttgc gaaattcaat ctagaattag gcaaactatc tgagtcgatc caagaacaag
     7621 aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc cgatcgtctg atcgaaaaaa
     7681 atcagcaagt attattagat ctttcggaaa aactgaagca gacagaagga caaaaagatg
     7741 ttttgcagga gcggacaaag catacacaaa aaagctcgca agaatatcaa acttcattag
     7801 cagaagcaca aaaaaaggtt aaacattttg aaaagcttca agaaagcttg atgaaagcag
     7861 cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa tctcataaaa acacagcaag
     7921 aattagaaaa ataccaaaaa tcaacgaaag aattattggc agaattaaga gatcaatatg
     7981 ttgatttgat gcaagaacaa gcagctgtag gaaacgaact gaagtatcta gaacgtcaat
     8041 atatacaaga aacagcaaaa agcaagcaga cgttagccaa gcaatcggaa gtagaagcat
     8101 cagtagaccg attgatctta caaaaagaag aattgacaca aaagcaggcg cagctaaaat
     8161 catctttaac tgaaacaaaa gagaagttag aaatgatcca acaaaacggg aaaaagttcc
     8221 aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt aatgaatcaa gtccagcagc
     8281 tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa ctattttggt ttttaccaag
     8341 gggttcgatt ggtcttgcag cataaacagc agctttcggg tattgtcgga gcagtggctg
     8401 aactgatcga tgttcctgca gatttcacgt tagctatcga agcagcttta ggtggggcag
     8461 cccagcatgt catcgttgaa aatgaaaaag atgctcggca agccatcacg tatttgaaac
     8521 agcaaagagg aggaagagca acattccttc cactgacgac aatcaaacca agacaattac
     8581 cggctcatat attgacacaa gcagcagctg tagaagggtt tatagggatt gccagtgaac
     8641 aagtcagtta tcccgatcaa atccaaactg tggttcaaaa cctgctagga acgatccttt
     8701 tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac gatccgctat caatatcgag
     8761 tggtttcctt agaaggtgat gtgatgaatg ccggaggatc catgacaggt ggagcgaata
     8821 aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga attgaaacag ttgactagtg
     8881 aatttgaaga agcagataaa caactccaag cacaagaaaa aaaagttcag gagctgcaac
     8941 aagaaacagc tcgtttagcc gaagaacaag aagttttgcg tacacgaggc gaacaattac
     9001 gttttgaaga acaagaagcg acaaatcagc tacaaaacat cataaatgaa cttgaacgtt
     9061 ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga actgcagcag tttatcgaag
     9121 attacgaaaa gcaaacgaac gaattgaaag acaaacaaac agatcttgaa tcgcaacgac
     9181 aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag cgaccagatg gaagccagaa
     9241 gagcacaagt acaaagtcaa aaagcacaag aacaagcaga tcttgctgtc ttgaaagagc
     9301 agtttaatca tctgcagatc cagctccgcg gtgcccgagt ccaaaaagca gaggcaaccg
     9361 aacgtcaaga agcgatagaa aagcagttgg cgacattgac agcagacttt tctgatcatg
     9421 aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt atctgctcag cgagaaacat
     9481 taaaagccga gttggcaaaa gccaaagagc agcgagatag aaggcaaaag gagatcgata
     9541 agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa agcacgactg tcggaacaat
     9601 ccaaattaga agtccaaaaa gaccgagctg aaatgatttt agataatcat ttgacctatc
     9661 tgcagtcaga atatcagctg acatttgaga aagcttccca agactatcaa gaaacaaccg
     9721 atatcgaaga cagtagaaca aaggtttcat ccttaaaaga acagattgaa aaactgggac
     9781 cagtaaactt aaattcaatc gaacaatacg aacaagtcag tgaaagacat acattcttag
     9841 caacacagcg agatgatctg ttagcagcga aaaaccaatt gtttgaaacg atggatgaga
     9901 tggatgatga agtgcggaca agattcaaag aagtatttga agctatccgt caagaattca
     9961 aaattgtctt tccaaatatg tttggcggcg gtaaagctga attagtactg acggaaccaa
    10021 ctgatttgct gaaaacaggt attgagattg aagtacaacc tcctggcaaa aaactgcaaa
    10081 gtctaagttt gctttctgga ggagaacgag cacttacagc catcgcgttg ctcttttcca
    10141 ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga ggtagaagca gcgttagatg
    10201 aagccaacgt gaaacgtttt ggtcgttact tgagcgattt ccaagatgac acacaattta
    10261 tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga tgtactgtac ggagtgacga
    10321 tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat ggaagatatc aatgaagagg
    10381 cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc aaattagtag caatcgattt
    10441 ggacggtaca ttattaaatg aacaaaagct gatttcagat gagaataaac aagcattagc
    10501 acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca ggacgcccct tagctgcgat
    10561 ggcgcattat ctgcaagaat taggactggt agatgaaggt gactacagca tcactttcaa
    10621 cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg gaaaagaaag tgatggaagt
    10681 atcagatatc caacgcttat acaaactggc tcagcaattg gatctgccac tagatgtttt
    10741 gtctgacaat gtggttttac agctgcctag tgccccaagg aagcaatcct tgtacaatgt
    10801 gttgaataag ctgctgcaat tccaaccagc ggatataagc gatattacag atgaatttgt
    10861 gcttaataag gcagtaatcg cttatgacca agaagaatta gatcctaaaa tcaaagaaat
    10921 accagcagaa ttccatgaaa tgtatgagat catcaaaaca agaagtatgc ttttggaatt
    10981 tatgccaaaa ggtgttacaa aagcttatgg tatttcttta ttggcaaaag atcttggttt
    11041 gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat gatttaccga tgatccaata
    11101 tgctggacta ggcgtagcga tggagaatgc tgtgcctttc gtcaaagaag cagcagattt
    11161 cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg atccaaaaat ttgtattaga
    11221 gtagggggag tcaaatggga ttttttgata aaatcaagcg tgcttttact ggcgaagaga
    11281 aaacagaaga agttgaagta caggagaagt acgacaaggg tttagaaaag acgcgtaaaa
    11341 catttggtga acggatgaat gagttgtttg ccaattttcg aacagtcgat gaagactttt
    11401 ttgaagaatt agaagaaaca cttatcggtg cagatgtagg ttttgaaaca gcgatacgaa
    11461 tcaccgaagc attacgtcaa gaagtgaaac tcagaaatgc gaaaaaatcg gcagaagtcc
    11521 aaaatgcgat catcgagaaa cttgtggatc tctatgaagc ggaaggaatc aatgaagtaa
    11581 atgaactaaa tatccaagaa gacggactaa cagttatttt gtttgtcggt gttaatggtg
    11641 tagggaaaac aacaagtatt ggtaaacttg cccatcagtt caaaaacgaa ggtaaaaaag
    11701 tattgatggc agcagcagat accttccgcg ctggagcaat cgaccagtta gttgtctggg
    11761 gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg aggcgatcca gcagctgtcg
    11821 tattcgatgc tttggaaaga gcaaagacag agcaggcaga tgttttacta gttgatactg
    11881 caggccgact gcaaaataaa gtgaacttga tgaacgaatt agaaaaaata aaacgagtaa
    11941 tccagcggga attaccagaa gcaccccaag aaacacttct tgtattagat gcaaccactg
    12001 gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac aacagatgtg accggtcttg
    12061 ttttgacaaa attagatggt acagcaaagg gcggtatcgt acttgctatc cgtaatgaat
    12121 tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat cgatgacttg gagccgtttg
    12181 atccaaatga ttttgtcgtt ggattgttca aaggattact aaaagaagaa taacccatgt
    12241 ctaaaaagca ggagctgtga caaaagttgc agctccttgt ttgaatgatt gtttttaaca
    12301 tagctgtgtt tttgatgcct tcatttttat acttatttgt aagcggttct caaacaattt
    12361 cgttcttttt cttagacttt ttgattataa ttagattcat atgaattgga ggtcacttaa
    12421 tgatgaattt taagcaatta ttattgcatg tcaatgcgcg cccttttatc gaccaagctc
    12481 gatttggtat agaacgggaa ggacagcggg ttgatcttgc aggaaatcta gcaaaaaccg
    12541 atcatccagc aatctttggc gatcgatcct atcaccccta tatccaaaca gattttagcg
    12601 aaacacaaac agagatgatc acccctgtta ccgattctat tcccgaatta tttcagtatc
    12661 tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa agaagagatg atctggccat
    12721 taagtatgcc acctgcctta ccggaaaaag acgaagagat cattattgca aaattaaaaa
    12781 atttcgaaga tgtcttgtat cgacgttatt tagcaaaaaa atacgggaag cgaaaacaga
    12841 tggtaagcgg tattcatttc aattttgaat ttggcgatga gttgctaaga acattgttca
    12901 gccatcagga agaatttcaa gatttttccg aattcaaaac agaactttat ttgaaaacag
    12961 cgagaaactt tatgcgctat cgctggatga tcacttattt attcggtgct tcaccaatga
    13021 gtgagaagaa ctacttttta gatgaatcac atccgcaaga acctgttcgc agtattcgca
    13081 acagtgcgct gggctatacg aatcatccaa atgtgaaagt ttcctatgct tctatgaaac
    13141 agtatttagc agatatcgag cgaatgatcg aagaagggaa actttcggag gagaaagaat
    13201 tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc agatttagca acaacaggtg
    13261 ttcgttatat tgaattgcgg aatatcgatt tgaatcctta tgcaagattg ggtatcaatc
    13321 cagagcaagt tcggttctta caattattcc tgatgtacat gttatggaca gaagaaaaag
    13381 aagactgtga ccaatgggta gcagaaggta cgactcgaaa taacaaagta gcactagaac
    13441 aaccatcaga tcaaacagaa tttcatcaag aaggtagaga aattcttgaa ggaatgaagc
    13501 aaatgctggt tgaattggat tggttggatt ctctttattt agtagaggaa gcattgactc
    13561 aaatggatca tcctgaacaa acgttagcag cgaaactcta tcaagaagca cagctatcga
    13621 gtcagcaaga agttgctgtg gcattgggac atcaatatta taaagaaagc catgaacgtc
    13681 cctatcagtt ggccggtttt cgcgaaatgg aactttctac tcagatattt atgttcgatg
    13741 ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc ggatcaattt ttgcgtctgc
    13801 aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat gacaagtaag gacagctata
    13861 tcgtcccact tatcatggaa aacaaaacag taacgaaaaa agtcttaaaa gaagctgggt
    13921 tccgggtacc aggaggcgca gaattttcat ccatggaaga agcggtaaaa gcttatccga
    13981 gatttgccga gcaggcattt gtgatcaaac ctaaatcaac caattacggg ttaggtatca
    14041 cgattttcaa ggaaggagct agtctggaag attatcaagc aggtttagcc attgcttttc
    14101 gtgaagacag ttcggtttta gtagaagaat ttatgccggg aacggaatat cgtttctttg
    14161 tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc tgctaatgtt attggtgaca
    14221 gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag tgaccctttg cgtggaacga
    14281 atcatcgtgc acctttagaa ttgatccaat taggtgaatt agaacaattg atgttgaaag
    14341 aacaagggtt gacgatcgaa tcagttcccc aagccaatca aattgtttat ctaagggaga
    14401 actcaaatat cagtacaggt ggtgattcga tcgatatgac tgatgaattt tctgaagctt
    14461 acaaaaaaat cgccgtttct gcggtagaag cactaggagc taagattagt ggtatcgatt
    14521 tgattatacc agataaagaa atcgatccta caactgataa aaaagcatat gggatcatcg
    14581 aagcaaattt caatccagcc atgcatatgc atgtttatcc ttttgcagga aaaggcagaa
    14641 gattgacgat gaatgtattg aaattgttat atcctgaagt attttaaaag aaaaagcttt
    14701 tgacagattg tcctatttga tgtcaatctg atcaaaagct ttttttgcat gtaaaaattt
    14761 atgtgttttt tcgttttaaa ataaacgatc ccagaagctt tttttctttt ttggtttttc
    14821 tttttcagct ggcgaagagt tttcttctgg gtattttttt tcgatatcaa tcacaggttc
    14881 atttactgct tcttttgctg ttaagagcaa tccgaattcc tcaggatttg cagtgacgta
    14941 atcgttgact acagtgaaac ggctattgtt tttcgttgct agctggatat attggttttg
    15001 gacagaaagg ggtagagccc cattcaagag tatggaagca tctggatgtt ttttgagttc
    15061 ttttaaaaag ttttctttgt tttttggttg gatcatttca gcaacagtca tactaagata
    15121 gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct tcagggttca ctaaaggtgt
    15181 accataaagt cccttatcca aatggctttt tacatcatct tgtgccataa aaaataccct
    15241 ccttcttctc tttgtctttc ttacgattat agcatattcc caataatacc taaaacatat
    15301 gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata agaatgaaaa tcattctcaa
    15361 ttagaaaggt ggattggatg cgctacggac gactagtagc attattagtt attttaagta
    15421 gtatttcatt gttcatcgga gtatcgaatg tacaattgtc agaactgctc cagttagatg
    15481 ggcaaagctt acaaatcttg ctagtgactc gtttgccacg aacgatttgt ttgatccttg
    15541 tgggagcgac cagtagtatt tgtgggttga tcatgcaaca tttgacccaa aataaatttg
    15601 tttcaccaac gactgcgggc acgatggata gtgcgcgttt aggtatttta gtggctatga
    15661 ttttcctacc tggtgcatcc ttgattgtcc gatccttgac agcattttgc tttgcttttg
    15721 caggaacatt gcttttttta tcccttacgc gattgttcct ccagaaaaat caattgatgc
    15781 ttccgttgat tggagtcatg ttagggaata tcattggttc agttgctact ttttttgctt
    15841 atcaattcca gttggtacaa aatatgactt catggcttca agggaatttt gcgactgtga
    15901 tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt gttgctcttg gtttggctat
    15961 ttgcttaccg atttacgatc attggtctgg gcgaatcgtt tgcagtaaat ctgggaatag
    16021 attaccagaa gatgcagttt ttaggcattg gcttagtatc attggctagt gcagtcatgt
    16081 tgatcatggt aggaagcatt ccttttatcg gagtgatcat tccgaatctt gtctctagaa
    16141 tatatggaga tcaagtgcac aaaacaattg gcgtaactgc tttagcaggt agtttgttct
    16201 tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc ttatgaaata ccagtcagtg
    16261 tgatcgttgg aatcatcggt ggggctatct tcctttatct tctgataagg ggacgacgat
    16321 atgcttaaaa aaaagccatc tatttttatt ggtatgcttg ttcttgtttt atttgttaca
    16381 ggaatgtact tgacgatcca tacatatgga aactggcatt ttgcatgtgc attgaggagt
    16441 aagaaaattt ttgcatttat ccttgtctcg ctagctgtca gcagtgccac aatcagcttt
    16501 cagacaatta cgcataacca gtttttgact ccttcagtat tgggacttga gtcgttatat
    16561 gtccttgtcc aaacggtcct tttcttcttt atcggaggag tgacgatgct tcagcagaca
    16621 ggtacagttt ttttcttatt gaacgttggg ctgatggtcg ggctgagtct attgttattt
    16681 ttctttcttt tgaaaaaaat gagagcgaat ctttttttat tactgatgtc agggatgatt
    16741 ttaggtacgc tttttggtag tgtcagtacc tttttacaag tcatcatgga tccgaatgaa
    16801 tatgatttgc ttcaaggcag attatttgca agctttggca atatccagag tcagtatctt
    16861 ttgcctgctt ctttgatcat tatcggctgc tggtacctgt tatttcgata tcgacatgtg
    16921 ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc tcgggataga cacagatcgt
    16981 tttgaatttt tgattttatg ttgtgtcagt cttttgacag ggacatcaac ggctcttgtg
    17041 ggtccagtta cttttttagg gtttattgtc gcaaatatca gttatcgtgt cttttctact
    17101 tatcgtcatg gtcagctatt tatcggaagc tttctggtag ctatgctgtt tctgacagga
    17161 ggacagctcg cagtagagca gattttcggc tggaatacaa cagtgagtgt cgtcgttgaa
    17221 tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg aaaggaagag tatcctatga
    17281 ttgatttaca aaaggtcaat aaactgtacc atggaaaatc agtggttaaa gatgtacagt
    17341 taacgattcc tgaaaagaag ttgactgctt ttatcggtcc aaatggtgct ggaaagtcaa
    17401 cggtgttgtc gatgatcagt cgtttgatac caaaagatac aggtgaaatc tatttagacc
    17461 ataacgaagt aaaggcttgg aaatccaatg aattagccaa aaaattatcg atcctacgac
    17521 aatcgaatga aataaatgtc caaatcaccg tgaaagaatt agtagaattt ggacgatttc
    17581 cttatacgaa aggaaaattg acgacaaaag acgaggaaat cgttcaggaa tcactggaac
    17641 accttgggtt agaagaaata gcggatcaat cgattgacac tctttcaggc ggtcaactcc
    17701 aacgtgcgta tattgcaatg gtgctagcgc aagacacgga ttatatttta ctggatgaac
    17761 ctttgaacaa tctagatatg aattttgcgg ttcaaatcat gcagatttta aaaaatttag
    17821 tacaagaaat cgggaaaaca attgttattg ttcttcatga tatcaacttt gctgcaagtt
    17881 atgcagacga aattgttgca atgaaagatg gacagttatt tacgcaagga acgacccaag
    17941 aaatgataaa aaaagatgta ttggatgaat tatacgaaat ggacatacgg atttgtgaga
    18001 ttgaaggccg cagattctgt ctttatttct aaaactaaca ttaggagaga agagatgaaa
    18061 aaattagtag taggtatctt gattctagca tcatttggtt tagcagcatg tggaaatagc
    18121 aacaacacca gtcaggcaga cacaaaaaaa agcagcacac aaactgaaac tacattgacg
    18181 ataacagata gtaatggaga ccaaatcgag gtgccaaata atccagaaaa agtcgtcgta
    18241 ttcgacaatg gttctttaga tacgatggat gctcttggtg ttggagataa ggtagttgga
    18301 gcagctacaa gcagccttcc tgagtatctt tcatcctata aaaaagtaga atcagcaggt
    18361 ggaataaaag aaccagactt agaaaaaatt aatcagcttc agccagatct aatcatcatt
    18421 tctggccgac aaagagattt tcaaaaagat ctaagtcaaa ttgctccgac cattttcctt
    18481 tctttagatg cgaaaaatcc atgggaatct ttccagcaaa atgtcactgc tttaggggag
    18541 atctttggta agcaagaaga agctaaaact caattagagg aattatctag tgcaatcgat
    18601 cagacgaaaa agaaagcaga agctactgac aaaaaagcac tagtcacact tgtgaatgaa
    18661 gggcaattat cagcctacgg atctggttct agattcggtt tcatccatga tttatttggc
    18721 tttgaacaag cagatgatca aatcgaagca tccactcacg gtcaaagtgt ttcttatgaa
    18781 tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag acagaacaaa agctattggt
    18841 ggagacgaca gcaaagatga tatttctgcc aatgaactgg tagcacaaac gaatgctggt
    18901 aaaaatcagc agatcatctc ccttgaacca gatgtctggt atttaagcgg gggcggttta
    18961 gaatcaatga aactgatgat cgaagatgtg aatcaagcat ttaaatagca taaataacag
    19021 aggattaaac tctcatacta tgtaacaata atgtttctta cattcttaaa tagcaagagc
    19081 ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg tattgatgat tttacggatg
    19141 gtttttattt atttgagaaa tcttttttca acgtgaattc attatctaca tcatacgggt
    19201 tgattctaga atggggagtg gcaaaaaata gtgtgatcca ttttttcagc atcaagatcg
    19261 cctcctttga tttgaatcaa aggtacaaag cgaaacttag ataaaactta aatttagata
    19321 tttttatttt gtaccgatct cttctttaaa cgatgttcca tctgctgctt ctcggcaata
    19381 agtccactag actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa
    19441 acaaatcgca gttgtcacaa tctcttctaa acgatgttcc atctgctgct tctcggcaat
    19501 aagcccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa
    19561 agcaaatcgc agttgtcaca atctcttcta aacaatgttc aatcagctga ccttcggtat
    19621 tagctcaaag cactgatttc acaacctcta tttaggcatg tgtctcagca agcggttgat
    19681 agttgatgat ctgcattttt cgataaaaat cggcgggaag cttactccac cgttttagta
    19741 attgatagaa ataactctct tctgctttga ttttatactc gcttaatatc aatccaaaac
    19801 tattcgtttt ttttgaagct aattttttat aaatatccaa tgctttggtt ttatcttcat
    19861 tgacaaagag tgtttttact tcaatataag aaccctcgat attttgtgtt tcttcgataa
    19921 aatagatgtt ttttatatat tccttcatag tgattcctcc aaagaagtct tttatttgat
    19981 aagtttactc taccgaagag ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa
    20041 tagttgttaa aggtttttta ttttttatag tattttttgt tatttaagaa aggaataagg
    20101 ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa
    20161 agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca
    20221 ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat
    20281 tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt
    20341 gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc
    20401 atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa
    20461 aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc
    20521 aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga
    20581 accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca
    20641 agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca
    20701 tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa
    20761 gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag accgttgcct
    20821 agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca
    20881 cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt
    20941 aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta
    21001 cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta
    21061 ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct
    21121 tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc
    21181 cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg
    21241 gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc
    21301 aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg
    21361 tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga
    21421 ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt
    21481 gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga
    21541 acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc
    21601 ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata tgctgctttt
    21661 tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac
    21721 tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatatacat
    21781 tttttattta attataatta atattgattg aagtctaggc agtcaagtca cattttgata
    21841 gataggaggg ggacttacga attattttaa aggctatgac agctgtattt ctagattgtg
    21901 tttgcatcaa gttgcaataa aaactagaaa tcaaatcggg ctgataatca tttataacta
    21961 atcgatacat aggaccaaat ataggtctat gttcttttat ttcataagta tcgtaaaaca
    22021 gaattttgct atggattaat ccaatcgact gatatgttta gtaaataaag aaagaggaat
    22081 gtcaatgatg aaaaaagaaa tcgtattaac agcagcaatg atgttattca gcatggtagc
    22141 aagtacgact tttgtttctg caactgaagt atacccaaag gaatataaca cagaaggaac
    22201 gattacattt gaagcaggag atgagggtgt aactcctcct gttgatccag agaatcctga
    22261 tcccaataaa tctgtcgatc catcagatcc tccaagccct ggaacaggag gggcattatc
    22321 gattgattat ggatcaaaat tcaaatttgg gactcaaaaa atttctactg cggataaaac
    22381 gtattatgca gctgctgatg tgatgaacga tgggtctaga aaaccgacat atgtccaagt
    22441 gacagatagg agaagtacct tatctgggtg gaaattaagt gtctctcaac cagaacagtt
    22501 taaaactgcc agtggggatg aactagttgg agcacagctg aaatttacga aagggcaggc
    22561 tgtttctttg gttgatccaa catatacgcc gcaaacggtg aattctgaat taacgttgac
    22621 accgggagga aacaatacgt tagcaattaa tgctaaatca ggaacaggtg tggggacttg
    22681 ggtctatcga tttggtgcaa atgaaaatga aaatcaagat gcggtacagc tttcggtacc
    22741 cggtaaatct gtcaagttag cgcaacaata ttccactaaa ttagtttgga cattagaaga
    22801 tacacctaac aactagttta ctgaaaagaa ctgttaaaaa taaatacgta gtcatattgt
    22861 cttgcgaggt aatcgagcaa aaattgtcaa aggctaggct aaatttgttt ggatacgaat
    22921 agttcatcta ggaatttaac gatcaggagg aaaagatata ttgaaaaaga ggaggatcgt
    22981 gctcatttta agcagtctct tagtgactaa tatctttccg atcatagggt actctttgga
    23041 attgaatcag ttggagcatg gaaatagaag agacgaagaa agcaaactca cttcgacatt
    23101 cgacagttta atagaatcca accagacaca agaaagcagt gaggatttaa gtatttcgag
    23161 tagtggagag accactgaca acgtagaagc tgagggttcg gacaaacaag gactagaaga
    23221 tgcaccaatt tctgcggttg agctgcctga cgatcccaat attatttcta tcgataaaat
    23281 ttttcaaaca cctatcggta ctagcacgtc catattggaa aatgggaagt tactgcagtt
    23341 gaacccagca gaaaggtctc aaagaggagc tatttggtca aataagaaga ttagtttact
    23401 ctcggatttt acatttaaat cttatttata cttaggaaat caacgagata gagcgggaga
    23461 tggcatgacc tttacattga ctggcgatcc tagaatggct agtaatccca gtcaagttat
    23521 tggatcgcca ggtatgggaa ttggctcata ttctactcgt tctggccaac cttacattgc
    23581 gaatgcgctc tcgattgagt ttgatacgta taaaaatagt ggttcttctg atcgaatgga
    23641 tcgagagata actgctgata atagagggca tggacatgtc gcttttgtca cgcctaaacc
    23701 aaataataat aattattctg gagagcatac tggcgtgacg gtagctccta catttctttc
    23761 taatggcaca tggcgcttgt taataattaa atgggacgcg gagcaaagaa gtcttactta
    23821 tgatttggaa ggtgtcggtc gctcttctcg gattatacct gatctcatta ggcagtttgg
    23881 ttctacagaa gtaaattggg ggtttacttc ttcaacaggt actttttttc aagaaaatgc
    23941 tgtcgctgtc acgcgacttc catcgaatgt cacatcaaca gctgatgtaa aagtcaacga
    24001 tagtgattat ggggccatcg ctgaagctgg aggaaacgat aagataacta tcagaaatac
    24061 attgaaggtc gagggggatt ttcttgaata taagaaaatg cagactagta tcgacctccc
    24121 acaagaatta actattcccg agcatatctt gatcaatgga aatcaaattg atgccaaaga
    24181 tatcttgata accggaaatc gagtcacgct aaatctgaca gattatttta ttgctggaga
    24241 tacgttaata atcgaattgg agacaacttt gcaggaagta attcctgaaa aagtactcac
    24301 tatgcatttt gaataccttg aaaacgggaa tttgattgag caatcgaatg aaataaacct
    24361 aaagatcgca aagctgaagg agatagatat ccatgtttat tataaagata aagacagtca
    24421 gattgatatt gctgaaatga aaacaatcac tgggaaaata ggagagaagt ataaagaaac
    24481 tcctattgat attgaagggt atgtttttga ctcggattca gcgaatacag aaggcatctt
    24541 ctccgaaaat acagaggata tctattttta ttacagaaaa ggcaaacttt atctctcaac
    24601 tgctcctcaa tatgtcaatt ttggaaaaca taaaatatcc agtagaccat taagtgaatt
    24661 cggacagttt tcaggtgggt tgaaaattat agatgaacgg gcttttggaa gttggaaatt
    24721 acaattgaaa caagagaaag ttcttacaaa tggagatttt gagcttccag aagctctttc
    24781 ctttgttacc tctgaaaata caacggttat agggacatcc gctgttacga ttttcgaaag
    24841 tgatcaaaag ggtgaaagcg atttgtcctc tcttttagat tctacagctc atcgtggaat
    24901 cagaataaat attcctgtag aaaaccaaag aatcggaacc tttgagggga aactatcttg
    24961 gatacttgca gatgtcccag gaaattaaca ggaagagaag aatataacaa aattattgag
    25021 tttcaagaag ggcacaggta ccataggctc ttgtgtccca cagagacaag attttgttat
    25081 agtcttctct tacatatgat taaaggagcg attttcttga gaccaaaaca acaattattt
    25141 ataacactcc tagttatttt aggggcactt tttaacaata tatcagtgtc agctagtgca
    25201 tttaattttt cagtgacgcc gataacttca gagaatcaaa tcgacaagag aaaaacttac
    25261 ttcgatcttc aacttgatcc ggatcaggaa gtagaagtaa aagcggaatt gagaaatgat
    25321 acagaaaaag aagtgaaaat cgatatttct gtgaatagcg caaccaccaa ttcaaatgtg
    25381 atggttgaat acggaaaaaa cgagattgaa aaagacatga gcctgatctt tgatctggta
    25441 gattatgtga gctatccaca gatagtgacg ctaaaaccta aaagtgtcca aaccgttgct
    25501 tttcatgtca ggatgccgaa tgagcgattt gatggtgtat tggctggtgg gatcacattc
    25561 caagaaatcc agacggaaaa ggaccaaaca gaaactaagg atcaaagctt atcgattgag
    25621 aatgaataca cctatattat tgctttgttg atgcaacaga cactgaacga agttgctccg
    25681 aaccttactc ttcatgaagt taaaccagat caaatcaatg caagaaatgt catcttagca
    25741 aacgttcaaa atgatcaaaa aacctatatc aatcaagtag ttattgaaac aaaaattaca
    25801 aaaaaaggac attctgaagt tttatatcaa gaggaaaaag aaggattgca gattgcgccg
    25861 aatacaaatt tttcttttcc aactgcttta aatggtcaac cgctaacccc aggtgaatat
    25921 caccttacaa tgactgtttt aggaaatgaa aatgaaaatg gaaaattttc gagaaaaaaa
    25981 ggaaatacta cgatcaacta taccaatcaa tgggtatttg aaaaagattt tacaatagac
    26041 ggaaaagtag ccaaggaatt gaacacgacg gatgttacga ttaaaaaaga caataattgg
    26101 atttatatct tagtagcttc acttttctta ttgtttattt tattatgcat acttttgttt
    26161 gttttgagaa aaaaagaaga gaaagaaaag taaggttaag ataacaaata gtaaattttt
    26221 attattgtag gatggttggg ctctttttat aacaatataa aatataccat tttattaata
    26281 aaaatgcata ctagtaaatg atataattct aataacaggt ttgatcgcga gaaaggccaa
    26341 ataaaacgct ctccaaaatg ttaaaaaaaa gacaggacga tgtatattat acaaacaaga
    26401 atcgctgaca acagtgactc ggatccagga aatcagtttt atccccagct agttggtgaa
    26461 tgcatggaaa gtaatatacg tagaaagagc tgatgactaa tgaattatga gtttttttta
    26521 agcaaagaag atcacagaag ttattcatta cttaaatatt tagaagcaag ctcaaattta
    26581 tcagagtcaa tatcgaatgt tcaagaagag ttgtcgctat cgacatttct cttgaaaaaa
    26641 acgatcgata agctgaaaca tgatttacaa aagttcgatc tagagaaaaa tttgcgagta
    26701 accgtcagtg aattggatat atgtctggaa attaatggaa aatgttctag caaagctctg
    26761 ttatctcgtt atataacgga ttctttatct atgaaaatgg tattggcttt ctttcaggaa
    26821 aaatatatct cagtagaaaa ttttgctaaa cagcatcacg tgagttattc tgtggcttat
    26881 aaagtattac aaagactaaa aagaaatcta aaaaaatatc aaattttttt tgaaa
//
LOCUS       DAYYAG010000029        26424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000029 DAYYAG010000000
VERSION     DAYYAG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.029"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1507
                     /locus_tag="ACHARQ_001046"
     CDS             56..1507
                     /locus_tag="ACHARQ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8409484.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1856..2488
                     /locus_tag="ACHARQ_001047"
     CDS             1856..2488
                     /locus_tag="ACHARQ_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8409485.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3076..4515
                     /locus_tag="ACHARQ_001048"
     CDS             3076..4515
                     /locus_tag="ACHARQ_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8409486.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4810..5028
                     /locus_tag="ACHARQ_001049"
                     /pseudo
     CDS             4810..5028
                     /locus_tag="ACHARQ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5277..5492
                     /locus_tag="ACHARQ_001050"
     CDS             5277..5492
                     /locus_tag="ACHARQ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409487.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5709..5965)
                     /locus_tag="ACHARQ_001051"
                     /pseudo
     CDS             complement(5709..5965)
                     /locus_tag="ACHARQ_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6152..7063)
                     /locus_tag="ACHARQ_001052"
     CDS             complement(6152..7063)
                     /locus_tag="ACHARQ_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8409488.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7082..8086)
                     /locus_tag="ACHARQ_001053"
     CDS             complement(7082..8086)
                     /locus_tag="ACHARQ_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8409489.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8083..10206)
                     /locus_tag="ACHARQ_001054"
     CDS             complement(8083..10206)
                     /locus_tag="ACHARQ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8409490.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10211..12658)
                     /locus_tag="ACHARQ_001055"
     CDS             complement(10211..12658)
                     /locus_tag="ACHARQ_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8409491.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12645..13034)
                     /locus_tag="ACHARQ_001056"
     CDS             complement(12645..13034)
                     /locus_tag="ACHARQ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8409492.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13094..13597)
                     /locus_tag="ACHARQ_001057"
     CDS             complement(13094..13597)
                     /locus_tag="ACHARQ_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8409493.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13610..13834)
                     /locus_tag="ACHARQ_001058"
     CDS             complement(13610..13834)
                     /locus_tag="ACHARQ_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409494.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13938..15848)
                     /gene="ltrA"
                     /locus_tag="ACHARQ_001059"
     CDS             complement(13938..15848)
                     /gene="ltrA"
                     /locus_tag="ACHARQ_001059"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8409495.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16594..16731)
                     /locus_tag="ACHARQ_001060"
     CDS             complement(16594..16731)
                     /locus_tag="ACHARQ_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8409496.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16728..17912)
                     /gene="mobT"
                     /locus_tag="ACHARQ_001061"
     CDS             complement(16728..17912)
                     /gene="mobT"
                     /locus_tag="ACHARQ_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8409497.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18171..18461)
                     /locus_tag="ACHARQ_001062"
     CDS             complement(18171..18461)
                     /locus_tag="ACHARQ_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409498.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18451..19593)
                     /gene="dcm"
                     /locus_tag="ACHARQ_001063"
                     /pseudo
     CDS             complement(18451..19593)
                     /gene="dcm"
                     /locus_tag="ACHARQ_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19669..19833)
                     /locus_tag="ACHARQ_001064"
     CDS             complement(19669..19833)
                     /locus_tag="ACHARQ_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409499.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19826..20500)
                     /locus_tag="ACHARQ_001065"
     CDS             complement(19826..20500)
                     /locus_tag="ACHARQ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8409500.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20578..21918)
                     /locus_tag="ACHARQ_001066"
     CDS             complement(20578..21918)
                     /locus_tag="ACHARQ_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8409501.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22001..22336)
                     /locus_tag="ACHARQ_001067"
     CDS             complement(22001..22336)
                     /locus_tag="ACHARQ_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8409502.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22346..22720)
                     /locus_tag="ACHARQ_001068"
     CDS             complement(22346..22720)
                     /locus_tag="ACHARQ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8409503.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22733..23047)
                     /locus_tag="ACHARQ_001069"
     CDS             complement(22733..23047)
                     /locus_tag="ACHARQ_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8409504.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23160..26387)
                     /gene="fss3"
                     /locus_tag="ACHARQ_001070"
     CDS             complement(23160..26387)
                     /gene="fss3"
                     /locus_tag="ACHARQ_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8409505.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7648 a         5519 c         4615 g         8642 t
ORIGIN      
        1 tatcataatt tttaatagaa aacactatta aagagaaaaa agagaggtaa atcaaatgaa
       61 aaaatcttta aaacttgcat cagcagttat attaatgtcc caactcctta tagcaacacc
      121 tttccatgtg ttggcaagcg aaaattcaac atacaaaatt gcaaaagaaa gaaccgagac
      181 gactgaaatg agtaattccg atgtaacagc agagagtagt gaacttttga caacttcatc
      241 aagtgaaagt agtacgactt caaatgccga gtcttcaaca acttcatcaa gtgaaagtag
      301 tacgacttca aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa
      361 tgccgagtct tcaacaactt cgtcaagtga aagtagtacg atttcaagta ccgaatcttc
      421 aacaacttca agtagtacaa gtgaaataac ttcttcgaaa gtgacaaaac caaaccatat
      481 agaatcttca aaaaaggaag cgccaaaaac ggaaggacaa acaaaaactt tttcgacacc
      541 acagcaaata gaatcaacag aatctttcca atcacaacca atactatctg gtaatgaaga
      601 aattcatttt gacaaaaatg aatctgtaga aaattttatt atgaaaattg gtgagtctgc
      661 gagaaaaatt ggtcagaaat atgatttata tgcttctgtt atgattgcac aagcgatctt
      721 agaatctgcc agtggtcaaa gccagttagc tcaggcaccg aattacaatt tatttgggat
      781 aaaaggaact tataatggaa actttgtcat tatggtaaca aacgaagatt tgggtaatgg
      841 aacgctttat acgacccaat caaaatttag agtgtatgaa aactatgagg aaagctttga
      901 agattatgcc aaattattaa ctaagggaat tagtggaaat aaagattttt atgcaggagc
      961 attaaaagct aatagtaaaa catatcgaga agcaactaaa ttccttacag gacggtatgc
     1021 aacagataca caatactatt tgaagctaaa tgaattaatt aaaacctacg atttaaccaa
     1081 ctatgataaa gaagttaaag gtccacaaat gagtaagact ggataccaag tgcctattca
     1141 taattattca ataaccagtt tgtttggaaa tcgtggaaat gaatttcacc gaggacttga
     1201 tatggcagct acacagggtg agccgattca tgcagttaaa gctggtaagg ttgttaaagc
     1261 agagtttcac tactcttggg gaaattacgt ggtgatacaa cacgaagatg gatcaaccgc
     1321 gttatatgct caccagcaac aatacctagt aaaaactgga gataatgttt tgcaaggaca
     1381 aataattggc tatgtaggaa gtactgggaa tagtactggc ccacaccttc atttggaaat
     1441 ttgcaaagat agtagtcttt ctcagagcat gttagttgat ccgaaaacct tattattcgg
     1501 agactaaaaa aggtaatcaa tctgacaaag taacaagaaa atggttggat tcttagacct
     1561 ttgatcagga tattgagact ggaattgata tctcgattac gatgggtttg acaaataaaa
     1621 caagtccatt atcgaatatc gagtgtatta ttgccgtctt tatgctcata ttttgagcag
     1681 gtttcactag atgaaaacaa tttatctatc ttgaggattt cgtgtacgta tcgttcagtc
     1741 ttgtattgta aattagtcat aaaatcagac taagaaaaat tggaaatatt tttttctgat
     1801 ttattaaaag ttaattgaaa ttatatggta cttctttctt gggagggtag gtgcagtgaa
     1861 ggaaagatat ggtcacctta ctgttgttga ggagattact cataatggat ataaaaaata
     1921 tcgttgccta tgtgactgtg ggaatgaaac gattgtatat cgatcaaact tactatcagg
     1981 tagaaccaaa agttgtggat gtggaaatta taaaaatagg cataaatata aaaatttgac
     2041 aaataaaact tttggtaagt tagttgcaat caagccaact aaactgcgaa gtgaaggaac
     2101 tgttgtttgg gaatgtcaat gtctgtgtgg gagaaaaaag ttagtatcgg caaagcgact
     2161 tctaaaaggt gatgtaaaaa gctgtggatg tttgaaatca aaaaagcatc acattgaaaa
     2221 taaaaaattc ggattactta ctgcaaagta tcctttggac aataactgtt catggaaaac
     2281 aaaatgggtt tgtcattgtg agtgtggcag tatgtgtgag ataagttata gtaacttatt
     2341 ttttggacat actcaaagtt gtggatgttt aaaaaggcaa gaaacaagga catgtgttga
     2401 agggacagta atagagtgtc taacgtcaaa attaccgaaa aataatacaa gtggtgttaa
     2461 gggagtttat cgttctaaag agaaatagat tgcgtatata actttaaaaa aaaagaggta
     2521 ctatttagga tcgtatgatt ctattaagag tgcagctcaa gctagaaaag atgctgaaaa
     2581 taaattgttt caacctttga ttgacaaata tcgaggtaat cattaattat tgtggataat
     2641 aattaacagt gatatataaa catctataat atattattca tactattgat atttagtata
     2701 atcctatgga attgaatatc atagcaatgt atttgaaaag caaattggaa aacaaatagg
     2761 tgcaacacca taaatatgaa aaaattggga aaaagtagaa tggaaatttg cttacgtctc
     2821 acgagatagc tcacactatt aatctgaaga acaatgaagt cgacttttag aaattgctag
     2881 aagaaagtaa agcgaattat cactagttac tatagagtaa gttcaaataa agcatctcca
     2941 acttacatgg ttacatgagg atatcaaatc gaaattactc aagcttccta tggctagagt
     3001 cgcgagaagt tgttgggcag tagacatagg ttatattgtt cttagctaca cgatcattag
     3061 aaaggaaatc ctattatgta tagttatcaa gaagatgcaa aagtccaaaa aaatcgctgg
     3121 tggattttga ttgctatatc tatgtttacc ttcatgtcca cattggatgg cagtattgtc
     3181 aatattgcac tacctaccat ctcagatgat atgggcatac caatgaatca agcggaatgg
     3241 gtagtgtccg tttatctgat gattgtctgt tcttgtttgt tgctgtttgg taaaattggc
     3301 gatagccgtg ggaaaatcaa agtatttcgg attggtacgc tgatctttgt cttgggatcg
     3361 ctgttgtgtg ggttcaatca ttcgctaggt attttattgt tcgcacgggt ggtacaagcc
     3421 attggtgcta gtatgactat ggctacaaat gccggaatca tcacggaagt ctttcccata
     3481 catgaacgtg gtcgggcatt gggagcaata ggtgcgtttg tttcattagg ctcgattgct
     3541 ggaccaggtc ttggcggctt gattttgtcc caattttctt gggcgtacat tttttggatc
     3601 aatgtaccgg tgggaatcgt aacgatgttg attgctgaaa aattcttacc aaaagatatc
     3661 accatgagcg gccatacaat cgatggaggg ggctttgctg ccattgcggc tacgattatc
     3721 agcttttttg gagcaatctt tttggggcaa gaaagtggct ttttccatcc agtccccctt
     3781 gtactgttcg taatttcagt gattgccttt gtcagctttt taaaagttga aaagaaagca
     3841 gcctctccat tgatttcttt cggtattttt aagaacaaga tttttaccat gagcttgatt
     3901 acagccttgt tgattttttc ttccaacttc tttgtcaatg tagtgattcc tttttatctg
     3961 caaaatgctc gcgggttgag tcctagctat gctgggctgt tgatgatggt ctttcctttg
     4021 atgatggtcg tcggggcgcc tttaagcggc tacctgaccg accgggttgg cccggagatt
     4081 ttggtattag taggcttggg cttattgtcg gtaacccagg tcatgtatat gtttttgtca
     4141 gtggactctc ctttgtggct atacattgtc gctactgcca tcatgggtgc cggcaatgcc
     4201 ttgttccagt cccccaataa tacgatggtt atgagctcgg tcactatcga aaacttggga
     4261 ctggcaggct cgatgaattc ctttgcccga aatttgggga tggtcagcgg gatttcgctt
     4321 tctacgacgg tcttatatca agcgatgtct gctaagatgg gccagcaagt aactaactat
     4381 atcgcaaatc ggccggatgt ctttttatat ggaatgaaga ttaccttttt aggcagcttt
     4441 gttttgtgtt ttgttgcctt tttcctgact atctggcgta tcaaaaaaca aggaggtatt
     4501 cagaaaaatg tctgaaaaaa ccactgttga agaatttttg gaggcctgcg tatgggtgag
     4561 tgggatttcc atggatgatg gaagatgaag tgaacttcta aaagccgaaa aactcaagta
     4621 ttatcttcaa aatactagtg ctcaatgaga tgcttgattt gatcacaaga agactgcgaa
     4681 agccgctggc aaccgcaagc taggtatccc accgatggac tacgttttag aggctactgg
     4741 ttatgaatac ggagccaacg ctctcatctg cataagaata catcacccag attatgtaaa
     4801 gattttcgat acttctgtca aaaaatcatt aagatagata aatgatttcc gtctagtcaa
     4861 attggttcag aatgtagaca cacagatggt aagaagtcac tcacaattcg agaatggact
     4921 tgtcctattt gccgtataca tcataatcga aacgtcaacg atagtatcaa tattttgacc
     4981 aagggctatg aatacaagca ttgccttaga aaacatcaag aaccttagga acaacgggga
     5041 tagcttggtt aataagagat accgctgttt aggcttgcaa gtatactctt ttatcaagaa
     5101 ttccgtggct ttagtcatga gaggcttaat actatgaaaa ttttctttag catttttgat
     5161 ttaggttcct tttctaatta taataagatg accactacag taacctactg aaatatataa
     5221 acagccactg tagtaccatt ttcaaagtag tctatgagag gagaatcttc attcttatga
     5281 acgctggaat tgcggatatg aatttaataa aaaaaaccct taatgatttt acttccaatt
     5341 caatatcaaa aggtactggt atcaatttaa gcacaataaa aaagctgaaa agtggtgaaa
     5401 gatcagtgga aaagctgaat cttttagatg ctataaaaat aacggaattt gctatgaaaa
     5461 atggaaaagc tgagattgaa atatggagat aattatcgtc tagtgaatta cttgccactt
     5521 agctgaattt aacgatcctt ggtgcttgaa gggggagatt cttgggaata gagcatactt
     5581 gcaagcctat gtctttacta acaacgatat ctcttattaa ccaagctatc cccgtagttc
     5641 taaggctttt acttttatct aagcaataat cgtattcaaa ggaaattttt gacccttaga
     5701 aagtctcatt ttatcagacg tgagcttagg cattgctgaa ttttgttaat tcggttttag
     5761 gttgtgcttt gagcataata tgaaagtatt ctttatcacg atttcattca tctaaggtaa
     5821 tgtgatgtga ttctgaaatt ctttcaaagg cagtttttgc ataagctgac attacatcat
     5881 caatgacatg tttacaatgt ttcactacta aaataagggg gtaaccaaga agaaaaactg
     5941 aatgattatt tgtatctaat tccatggtat atcaaccatt tcctatatag actgattatg
     6001 acatggatta aaataaaaag gcactatgtc ttaattgtcg gttttcgagt taccgaatgg
     6061 cattcatcac ccacctatag aggatgggcg acttctgcct gaaataagtt acactactta
     6121 taacttctaa taaaattaga ttgagtcatt attatttaac atcaacgatt ttccagtttc
     6181 catttttttg taatactaat ttgtactgaa agtagctagt ggtttttgat acattatcaa
     6241 ggtattttat ctttgtactc actagaaaat tttttccgtt ttgtagataa actggattct
     6301 ctaactccga aaactttaag tcaatattga ttggtcgcat cgcaccattt tcaacataat
     6361 actccaattc tttttcagaa gctgatggat aaagtttaaa aaatgtttct aaaaaatcgg
     6421 tgatttcttc cgttgttttg gcatcaattt ctgaatcgtt ttctacttgt tttgcttcat
     6481 attttgcctt gtctggcttt ccaatcattg ttggcaaact agttactaaa tgattcctgt
     6541 tttcatcttc aaagattgtc actcgatatg cagaagaaat tgattgcgtc ttttttccct
     6601 ctgtaatatg ttgctccact aaaaatgaaa cgacaaattc ttccgagtgt ttttcaacat
     6661 ctagaatttt tacagattga acttcggaac tcgtgggaat atctgctcga accatatctt
     6721 ggattaaagc gagaccgttt tctgtaaggt actgttcgag attactcatt ctcttttcaa
     6781 ttgcttcttt gttatttttc catgaaaaat attctttcgc aaaatcctct gtaaaacttt
     6841 caacgccaga agtattcact accttctctt ttataaccac tttttcatga gttgtatgtt
     6901 gatcaatggc ggtgaaattt ttataaacgc caaaagcaag actacaagac aataaaagcc
     6961 aaagggcaag caccattttt cgatgcttac ccacggatac aactcgttct tttttctttt
     7021 ttgggctctt ttccttttga ttccgttcaa tttttatctt cattcttttt cctcttttct
     7081 cttatttgta tcggccagct ccgataagat gctgccgcca atagttttcc gttagattcg
     7141 tccaccccac tggatctcct gcatgaaaca tgcgattctc ccctgcgtaa atgcccacat
     7201 gggtcacata agtaccggca tcataggttg aatgaaagaa aactaaatca cctggtttgg
     7261 catcttttag atcaatatga gtcatggcat catattgatc ctgagccacc cgaggaagtt
     7321 tcaggtcaac tttggcataa caccattggg tcaagccact acaatcaaac cccgtttctg
     7381 gacttgagcc accaaacaca taggccgttc cttcatactt aaatgcctca tcaaaaattt
     7441 cttggactat tttgtcatca aactgagttg tagttaggta ttgcttcact aataaacagt
     7501 aaaacatatt gccataatta taccgccacc caccattttc cttaatcgcg attgggttgg
     7561 ggtaatcgac tttttgacca cctgattttt cctttgaaaa agattcagct aaagcaaaac
     7621 tatgcttctt cccgtttttc ccgacatgat ctagataccc gccaccatag ttataggcct
     7681 gaataatact atcttgatca caccctagtt ctttggaacg cttaaccaaa gacgcaaaat
     7741 agagacagcc ttgtttgatg gatgcctcgg tacttaatga attaggtggt aatccggctg
     7801 actcagaact ttgcataacg tcttccattg tgccaccaga ttccacttga ataatcgcca
     7861 aaagaattgg aatatactct gttatatccg attctttggc atacttttcc accattgcac
     7921 ggtgtttcaa tacttcttct gagacactag ttccgccagg agaagtgcca gaactaccat
     7981 ttccctcact gtcttcacca aacaacaaac ccacacacat caaaataccc ataaaagaga
     8041 tggtcagaaa agttaatgaa ataacaccca ctttcttcaa gttcattttt tctcacctga
     8101 ccttgatttt ggacgatggt tcattctttt ttgtactttt tgattggtct gctttggcag
     8161 agtggacctc tgtttcactt gttggaaaga atttttctta cttgctgatt ggcttttccc
     8221 ttttgaaatg atttcttgac tagttagttt tcgtttgatt tcgggattca gatttgctct
     8281 cacaatccgc tttggggctt cttttttcag catcatatct ttctgaggtt gagctgcttt
     8341 atctattgga atattcgccc ttctttttcc ctgacgattt gttgcttcac tcagtatctt
     8401 gcgtttttcg tccattttcg catttcttcg ttgtcgttgt ttttctcgca aatccactcg
     8461 atccgctttt tcttggaaca gtccgcgctt gaattcttct ggtgcttttt tcgacttttc
     8521 gattccttta tggagattgt atttcaaatt cgttggcgta ttccttacct gatcctttac
     8581 ttgtttagtc ttatctttca ccaaagcttg ggtatccaaa actttgccga tttttctccc
     8641 agccaaattc attcgggatt gtttcttatt atttgaaact ggattctcct tagttaagtc
     8701 agatagtatt tcgtgatcgt tcggtaatcg tgaattcttt cgtagagaag aaccggatgg
     8761 cttaaatttg gattttgatt gttcctttgc caacttattt ccgactgctg caccaccacc
     8821 aaaagccaaa gctcgtccca catttcgatt gagttgccgc aattttcgat tcattaacat
     8881 ttgcggtttt cgcatgacgc gtcttcccat tgactggcta tcattactct ggaggttgaa
     8941 catactcata atgtcgccca atttgaaata gataccggca aaggtcacaa tttgcagaaa
     9001 agcaaccatg aaaaatggat aggttgccga aatattgaaa aacatcgtgg agatgctaaa
     9061 tgcagtcgta atcaccaaag taacacccgc tcgcatcata atcgtattga ataaacgaat
     9121 aatcgccttc tttcctaaac tttcataagt cggcaacatg gacaataaga aactaattgg
     9181 cagaaacatc gcaaagataa tgaagagaat ttgcgagaac aacataatcc ctgttagaag
     9241 aaaaacaaag aaggaaatgc caatattgaa caataccaaa aagaccacgg tccctaaacg
     9301 gttcatggtc ttggtaatac tgagattcgc attgtcattg tcttcaatct ctgccttgac
     9361 cgcttcttcc cgatctttgc ctttgttttc atctggactc acagatagaa tcttgttgac
     9421 ccggtcttca ccaatttctt ctatgttgga atcatcaaat tgcagtagta accaaggttg
     9481 ttggacttga atcgaaaaca aactatcccg aatcaaatcc acactatctt ttccttggct
     9541 ttctgaattg ggtacaacga tctcggttcc aagggtaagg gccgcttcac taacatctga
     9601 gctaaagtca ttgatttttg tgatataggt aggcgcataa gcaatgaacg aacccgatag
     9661 tagaaaaatc atcacaaaat taatcacggc tcgaactgct ttcgtggttt ctcgttttaa
     9721 taatccggta tacgccacat acacccccag agctaaaatc atcaataata gaaacccaaa
     9781 ataaaatccg gaggtctgga gtccattttc agtaatacca gccaatgttt ggatattctt
     9841 tccaatactt tcagccgtat ccgaaatgaa atctaatttg taagcctctt gaaccacata
     9901 acccgtggcg ttcgacaaat acaaactcac cgtccaaatg aaattagtaa tggcataaag
     9961 accatacatc acgcttttcc ctaagccatc accccaattc caaggcaacc aatcccaaga
    10021 gctatccaca aagaaatcta gttgatagtt gttcagtgaa tatttggaat atagatttcc
    10081 tgcttcaact gtatcatcaa ctagccctgt cgcctcggct accgtaccaa ctaaactcaa
    10141 tagaactatc agagtgagta tgctaataaa aacgattccc acaatccgaa aaagtttctt
    10201 tttcatcccc tcactccatt ctctcttgaa caggtggacg ggtatcaaag gcatggaaga
    10261 gttcttcaaa gactggatgt acctgaacca cgcctacccg accatacaaa tcctgcatca
    10321 agcattggcc attttccaaa tctcgtaggc gtttttgatt gctttcatct tctcgatcca
    10381 agccaaagaa ttccaaggtt ttcttgattt catgaatatc ggtactgcga aacgcaaact
    10441 tcaacccgat attattcttc aagcgttcat ccagtaaatc atcactattt tgcgtgacaa
    10501 agtaaacccc ggcattcata gcccgcccag cacgaacgag cttattcgat aatgttttcc
    10561 cttgcgctac ttgaagaaaa ctccacgcct catctaagtc tacaattttg aagatttctc
    10621 gattactatg gataaagtcc aaggcaaaag tggaaatgac gattagcatg gaaacactaa
    10681 gcaattccat ggtggtatat tcttcaaaag tggtttctgc atctggtaaa accaaatccg
    10741 ctacttgaat gatatttaac tgcctatcta aactaatcga ctgggtaatc gaaccatctg
    10801 agaaaagcaa atgggcaaaa tcataatcgg taaaggactc gatgtgatcc gcgatatttt
    10861 ccgcaactgg tgagccttct tttcgaagct cattaattac ttgtaataat ccccgttgat
    10921 tgctttgggt aaccgtccga atggccttac gcaacactgg aaacttctcc ccatctcgac
    10981 tagaaattcc tgttaaaaag gtcagaatat caattgctag actttcagaa tccttctttt
    11041 tctccataat cacataagga tcaagtaatc cttgattttt tggcttacta gttaagttga
    11101 gaatattgat ttcatccgca atttctggca aggtttcttt ccagccaccc cgttcccctt
    11161 ttggatcgac aatcacagct tgtccaccaa aaaggacggc gtaataaact aagagattgt
    11221 tactaaagga ctttccgcca ccgagtgaac cgaggaatgc cgctgctagc gcattggtaa
    11281 ctgaatcctt gaccccttgt gaggccaatg ccggtttcag gtaaacattg cgccccgtat
    11341 ctacgttata accaaagtaa atgccttcca actcccctaa catctgagtg gcgccaaaac
    11401 caagtccggc gagaaaatca gacgtaacat actggatata atcattcatg tatcgcttac
    11461 tagaaggtaa gaattcttca tgtagcccca tcatatcgcc aaaaggccgg actagtttta
    11521 cattggtatc atcataaaaa tctagcactt catcacaccg gcgctttagt tcatctactg
    11581 attcggcact tactcgaacc acatagctca gtttgtacat ggattctttg gactgatcca
    11641 aggtagtttc caattcatct acggaatcca atgcatctaa gacattggaa tttgtttcat
    11701 tgtcagattg ataggcatgg ttgtccaagt cttttaattc ctttttctta ttgcgaacgg
    11761 tagcaagtgc ctttttgttc gtcacaatct ccacattcat actagtgtca attgggaatg
    11821 taaattgttg ttgctggtaa tagaaaagtt ctgaggaggg aaactccatt tcccccacaa
    11881 ttgtattaat tgttaagtac gctacatatg attcatgatt ctcatgttcc atgcataagt
    11941 accggggctt ttcttcaatc aaacaacgac tgggacgtaa taagtcgtaa cgtttcacca
    12001 aagtttccga cttcagcttt ttctttggta gctgaaattc atattcttca aatggtgtac
    12061 ccttttcgcc atagatatgt tcaatcaaat acatcagatc actcggtgtg actcgtctga
    12121 ctttaaatct tcgagccaat ttactttcca ttagtttttc aagcttggta tatcggcgaa
    12181 tttcttcatt ggataaggaa acaaaatcgc ccatcaaatg atgattcact ccatagacaa
    12241 attcttgaaa gccggagaag aaagatttct tgagattctt tagattgacc tcttcatctg
    12301 tcgcaatcag tttgaagcca ataaaaaagc gataatcaat ctgactatcg ccaatcatgg
    12361 atactaatgc ctctgtctgt aaatcaattc gttgtttcgc tacttctgtt aatcgcccgg
    12421 taatttcctt ttttgatcgt tcttgcgtgg cacgaatact actttctgtg gcaatttgta
    12481 aggcatgaat cttgccttca cgattttgcg caatcaattg acgaaagtta tcgtggactt
    12541 gatatttctg ttcaggtgac aaaaaggagt aattgtaagg tattaactca tagtaggcaa
    12601 aacattctcc ctcttgatta aagacaagat tattttcaat atacttaatc gggtacatag
    12661 ttcacactcc ttaccagagt aatgggttcg tcccatcgtt tcttctgttc tttgactttc
    12721 tttcctgcga aagtgacctt aaatcgtaag tgatagctaa taaaagatcg taaaaatccc
    12781 aatggtttct taccgtcaaa cgttttttgc gacatgaacc aagtaactgc caccggtata
    12841 cctaagtatt ttaaaaaggc cccctctatc atagatagtg gaggcacatg ggcaaatacg
    12901 atgacaaaca gcaaggataa aacaaaccaa gccatctggt taaaagtcac aggaaaaggc
    12961 agttgaaagt cattaattgc ataaatgacc ttttcaactg cccaaatact ggtgtagctt
    13021 tttattttct tcatcaaatc attcctttaa aaagaaggat agtttagaaa tcaactaccc
    13081 ttcaataatt ttgttaatat ggatactcaa aaacacctcg actcgttacc aaataattgc
    13141 ctccaatctc caagtctcta ccacacgcat caaaatcaat atacgtcttc aagtgttctg
    13201 gtacttctcc aaaatcacca gcttctacaa tcaagaaata tgctacatca ctcatatcat
    13261 cgcaatctgg ataatagata atatcatcca catgctctac catctcctca aaagaattga
    13321 agaaagcatg ttgaatctca gaagcaactt caccaactgg cgttccttca agttcctccg
    13381 ctaagccaca caaccgatta atttcctcaa ttggcgtata ctcatcaatc tcaaagggaa
    13441 gttcataatc atgaattgca tattcctcgt attcgtcatt taacccaatt cgttctttca
    13501 cttcatcaaa atcgacaggt ggggtaaacc acgcccccac caattcacct tcgttatatt
    13561 tcccgaggtt cgcaatataa acacgcatct gttccatcgt gcttccccct tacgcaccga
    13621 caattcggtt aaacaaactg agtaagacat ccttcacccc agaagcatta aagaccaatc
    13681 cgaccgcaat caaagcaatc accaaaaagc caatcagttt ggaaaactcc cgcttaaatc
    13741 ctaaaaacaa accaatgacc acaattgcca gtaagactaa ggattgggca ttggttaaaa
    13801 accattgata tatggatagg taagttcctg atattataaa tatctcaaac ttttcccccc
    13861 attcacacgg agcgtgcgac tttcaccgca ctccgcgttc catcgtgttt atattgaaaa
    13921 gtatttatgt agtagaccta tatcaagttt aattcttgat tgccagctaa tactctatat
    13981 ttatttagtc ttatgagctg gactttattt agcttcaata gagctatata tttttgtatt
    14041 gtttcttctg ttttagcgtg tattagacgg tgtaccgcct cttttaccaa aataagattt
    14101 ttataatcgt catttccacc agctccagtt ggtacaatat gatgacaatg tatttcatta
    14161 gcttgttgaa attcttccag agttatagca cattttccat gttgagcaga aaatagcgaa
    14221 attcgattgt ctgcgtattc tattgtgcga ttttccaaag ataatttcat taactggtac
    14281 atcagtaatt tatcaacact tagattggtg tggataaatt ttcttccctc tagagtgtag
    14341 caattcgcta aatacacctt tgccattggt tttctgtgtt ggatatatcc aacagggaaa
    14401 ataggttcgc cctcgccagc aacataccga agcattttag atttaccata tcttgttgcc
    14461 tcctttgtcg taagttgtct gcctgttttt ctcaatctgc cccttttgtt tgtacctaat
    14521 ctgttagtga agatagccat tacagctcta ttcagtttca tacaatccag atttacattc
    14581 gttgcaatct gataataatt ttgcatacct aataccattg aattataaag tcttgtttct
    14641 tctaattccg ttttcccttt tgagggagaa gcaattttct ttgcctgtga taccagcttg
    14701 tctttttggt ttttgaattg tttatcactt atatgtgatt taacaactaa cttctttctt
    14761 ctcggtatca ttttaatctt gaaaccaaga aagtcggagt atctttttct aacatttaca
    14821 attcttgttt tttcttgtga tacttccaat tttaatcgtt gttcaatcca ttgagtgatt
    14881 gctattttgg ctttttcagc actttccttg tagcggcaga aaactctaaa atcatctgca
    14941 taacgaacca tatacatttc ctttaacttt gttttcttca tttctttgta agcgttgctt
    15001 ttacatgggc taccgctttt attgatatga accacatagt tttttgtaac aggattttcc
    15061 tgccattggc tttctaccca atggtctaac tcattaagaa caatgtttgc tagtagtggg
    15121 gatattattc ccccttgcgg agttcctttc tctggatatg ttattgtacc gttagtcatt
    15181 ttgattggag cttttaatat cctttttaaa atgaatatca agtgcttgtc ccgtattccc
    15241 atagcccata tctgttttat cagcttggaa tggttcacgt tgtcaaagaa acccttaata
    15301 tcaaattcaa tcacataatg tagctggctg atttgtaggc ggttataggt tgcttttatt
    15361 gcattttcaa ctgacctatt aggtctaaaa ccatagctgt tttcactaaa tttagcttcg
    15421 cagattggtt ctaatacttg ttttatacat tgttgtataa gcctgtccca catacaagga
    15481 atacctagag gtctggtttt accatttggt tttggtattt cttttcgcct cactggctta
    15541 ggtcgatacc catgtggact gcctgcaaca atgtatctta cttttttgac aactgtttct
    15601 gctggcaatt tgccaatatc tttaattatt gtaccgtctg ttcctgctgt tttactgcct
    15661 gcatttgctt tgatatttcg atatgccagt aatatatttt ctctcgatag gataatatcc
    15721 ataagatgag tgaaaatatc tccattctta cttttggcat ataaatcatc aaaggtgttt
    15781 tgcatttcgt aatattcagc gtgtcttaaa tcttctacac acaatgtttc atttttattt
    15841 ttaggcataa ggcttcactc ctttacttga aattgagtga gcttttttat cttactcgta
    15901 atccttattg aaacaacata atacttttct aatatataca cgacttgagg gcattcctcc
    15961 attctcatta caagaatttc ttaggttcgc cctctgcttt tcagcattag ttcaactgct
    16021 tattgttctt cgtcagttta ttctcttgat gagaacccaa tacctttcct cgttccgata
    16081 atcttatcta tacatatata tccttaggtc actagctata agcctgctgg cttgataccg
    16141 cctgtaacgt tatatggcat ttcataacct atattcttac tttgccacac cagctacgat
    16201 ttctcgtacc tttgcatttc tacaaagttc aaatttagac ccgtacattc gctgtttcgt
    16261 cagttcctta tagaacattc taaccataga tattttatag acccccgacc tgttggatac
    16321 gctacccacc tctggatagt tttcgataga agatatatgt tcatcaacta ttacggtatc
    16381 tttcagccgt ctttaccgag cttcaaacat tctgagtttc ctcatacaat gcttgtcgga
    16441 ggctcagggc aggcgtttca gagcgttact tcatcattcc acctgtcaga ttatcagttc
    16501 tcctaataac caaacttttt cgttctttta ttagtgcctt agcttttcac taaggaacga
    16561 gtcgcacata aattttgtcc gaaattcatc ttgtcacttt cctttctttt ccattgctcg
    16621 atccgctgct gcacttgcat tcatcaagca catcacagta acaccaagca tactgccacc
    16681 aaataccaaa aaaatatctt ttacaattcc catgacgatt cctcttttca tataatcaaa
    16741 ttttcaattg ccatactttg ttgctccaaa atctttttat gtcggtctga taagcgtgca
    16801 ttctgtacca tatcctggat ataggttgtc cgattcaatc gatccaactc cttagccatt
    16861 ttccacgtag gggcgacttg tcggcctagc caattcaagg ttcgttcaat ggtatacggt
    16921 tttggctcgg aagttaattg aatctctttc cgattccgtc caataaaacg cccccattgt
    16981 tcattcaagg gccagttggt tcgcctttta ccttctactt tatccgcaaa tcgcaagtaa
    17041 cgattgataa tggaaaacgt ggtgctctca atgctacgac cttttaacaa atcttgaatc
    17101 gcatgaaaag cacggtcatt tttcagtcga atctcaaaac gatttttcgt ctcagtttgg
    17161 tctaatggaa tccctaactt gatatattgt tcatagtctt tttcatacaa acagaaataa
    17221 acctcactct tgagactgcc gatatataag gtattgccca tgccgtcctt ttcatcagcc
    17281 tttaaaagtt ccccggaacg ataactttta aaggtacgaa acaaggagat acattcttcc
    17341 ttctgacatt ttttcgttag ctcaggtata tccaatagcc ccactcgatc attgattgcc
    17401 aagtccaagc gtttcatcac gccaccggcc tttagacaat cctcgaaaaa gtcgtaccag
    17461 ctacgctttt gagcgagtaa aaaagtttca aattgtcggc aacctcgccc ctttaattct
    17521 aaaagcacac ctttatcttc ttcatgagac aacatcacca caatatcacc catgacatac
    17581 tgttcttgat aggagtaaaa ggcataatct tcatggagca tatactcaaa tttcaaatgg
    17641 agaatttctt gaataatttt tcgtgcatcc gttgtgggaa agcgaatcct tacatagtca
    17701 aacagtaacg tcaaaggcaa atcgggatta aagctttcca gttggtcaaa aattttcctc
    17761 atggttttta tagatgcggt tacgccacca ttttctatct gatttaagta cggccgacta
    17821 agtccacaag ccgtagccaa tttattttgt gaaacgccgt attcgttgcg tcggtctttt
    17881 aatagccgat aatctaaatt ccgttgtgcc aagttctcac ctccaaattc aaggtgttaa
    17941 ctttttcaaa tgagttaaca cctatacaaa ttacttacta tctttgatac aacaagcatt
    18001 cttaatggtt gcttacttat tttttgtgtt tttgtacccc cctgttagat acgggggtga
    18061 gacatcatgg tcggctaacg ccgtccatga acaggcacag caccacggag gatttctgct
    18121 tcgcgaatcc tccgcggtca gcgcctaaca gtcagttgtg ttggtttagg ttactcatct
    18181 gtaataccat ttacttccaa ctctgctttc atttctaaca agtattgacg aatatctggt
    18241 tcttttgctt ggtccgcaat ccgctcaaat tctgcaatta cttgatttat ttcaattcga
    18301 gcatccgtct cctcattcat taaatatgga tcgccagcga ctttgacacc attcttgcga
    18361 atatcaaatt ttaaccgtcg caattgttct tctaattctg ggtctgactg aatctttcca
    18421 atcacccaat aaatttctcc aatcaactca tcataaacca tgtacatcca actcttttaa
    18481 tcttctggca atatcaaaaa tgaccggaac tgttacagag tttccggctt gcttatacaa
    18541 ctgactatct gaattgactt gagaatctcg atcaaaagcc caatcgggaa agccctgaag
    18601 tcgccaacac tctctaggtg ttagcttccg aatttgatac tgatccgtga cgatcgcttg
    18661 ttggtctcct gttagtaagg tatgaacact ctgttttccc agacgacctc ggcgacttgt
    18721 ggaattagga aaagccagat tcacactgtc ttctggcttc acctcttgat attctgtttc
    18781 attttttcga atcatgattt ttgtaccacc ttgtttcgtt gacaaggtgg gggcggttcc
    18841 agtaacttca taaactcgat taccttgttc aaactttccc ggtaatagtc ctgctacaga
    18901 gattccatcc cttgctggca gtgcgatttt gattccttca tcacctgtta aggttggcgc
    18961 tacgccatct gaaccataga cttgtccacc cattcctcga ttggaaggat tgatatttac
    19021 caaatttctg atgttttgat cagatgttga agacggtcca atgataggaa atacttgttc
    19081 ggtacatgcg tctcgagaat gtccgataag gaatatccgc tcccggcttt ggggaatgta
    19141 ggcctttgag ttaagcacct accactccac gtcatacccc aatccatcca gcgttcggag
    19201 gatcgtctcg aacgtagccc ctccttcgtg attgagcaat cctcggacgt tctcaaggaa
    19261 taaaagctta ggtcggagaa gagtggtgaa ccgagcgatt tcaaagaata aagtacctcg
    19321 agtatcggaa aatctttgcc gctttcccgc aattgaaaaa gcttggcacg gaaatccgcc
    19381 gcaaaggatg tccactggtc cgagggattg aataaactca tctgatacac ttgtgatgtc
    19441 atgcatttcc acctccttac ttgtatcatg aattgcttta taactacgtc tagcaaattt
    19501 atcaatttca caaaaaccga tacaatgatg gctcgcctgt tccataccta aacgaaagcc
    19561 accaataccg gcgaataaat ctaaaaatct catacattac ctcattcatt gcaaagaaaa
    19621 aagccaagaa cattttgctc ttggctcata cattattaat ttgtcctact attttttatt
    19681 taatccaact aatttccgtt gtttttcatt ggcctgatcg gctacatatt taatcaccgt
    19741 tttgaactct tttgcagtag cattttccga taaatccgca atgctcttat tgtatttctt
    19801 tacaattttc gttacttgtt ctttctcagt catgtaattg aacctccaaa tctttcctac
    19861 tataccctag tggtaaaaat aattctagat tatccgtagc ataatgaact tcggaaatat
    19921 tattcgtata atcttttatt atgatatgaa gcaatacatc tggatgttta ctctttactt
    19981 ttgcaatgtt ctctcttgat ttgaaaaaag cgttaataaa tgcctttttc ggcacatgac
    20041 gtccctctgc aatttcacgt tcttgtgtaa atttccaagc gattttaggg tcttgataaa
    20101 catagaaaat cgtcactcga aaatgttttt tcaacgcccg cgagatattt ttttcagcac
    20161 ttaatattgc aaaagttgca tccagaataa aagaatagcc atgatctaag acatatctaa
    20221 atgcctgctc taccaaccaa gaagatgctt tttgaaagag actagagttt tttccattat
    20281 aatcaggaaa ctgggtgcga aagtaatccg catcaataat cacatggtta tcataattct
    20341 cagctaatcc aatggcaacc tctgattttc cagccccagg actccctgcc ataaaaactg
    20401 cgtctcgtcc aacttcgggg gatttccctt tgataatata agaaataaac ttatccttat
    20461 ttgcttttgc atactctaaa taagaatcat tactttccat attaattatc ccctttcttc
    20521 ctactatata aaagtattat acgttacttg aagtataaag tcagtattga agatcactca
    20581 atcattcatc ttttctaatt tactaattga atctaagaaa tcataccctt ttggtacaag
    20641 tggcgtataa aactcactaa tcacactgcc acctgtatcc acataacctc gacctttgat
    20701 gtgcttcata aagaagtttt tttcgacctc cccaaacatc atggaatatc cgagttcact
    20761 catacgaccc aaagccacgc gaaagttgaa ttgatcgcgg attccgtctc ctaaatattt
    20821 cgcatccggc ctttggcaag ccaaaatcag aaagaaacca gattgacgac ccaacatcac
    20881 gatttgtttg agcttattca aaatcaccgc actttctttc gtcgttaaca tctccatata
    20941 agccacgtat tcatcaaaga ttaaaaagtt gggtggaagt ccaagatagg cataattctc
    21001 tcctgttttg tagttagaca tttctttcat tgccttgcta cgagccatca tacgttcata
    21061 gaaatcttcc acacaagcag aaatttcttc cttttgtgaa taaacatgag gcatcaccgt
    21121 acctaaatca gctaaatctg catttttggg atcgaggata aacaattctg catctgactt
    21181 caatagtgct tcgataatgg tgaggaggaa ataggtcttc cctccacccg tgccaccagc
    21241 gattaacata tggggtaaag aatcataagc ccatacttga tttttcatca atcgcagagt
    21301 cccattttct gccactactt cgtctatccc aattcgattg gcgatcatat catacagtaa
    21361 agtatactcc acataggagt ctttgagttc cttttctact aactcacaat acagtccgct
    21421 ttctaacttc ttttccaatt ttaaaagctg atcttggtat ttcccaagtg aaatttgaac
    21481 gcgaatagac agtaatccat ctttcattcg ataacagatt ttggggaagt aactgatagt
    21541 ctctctggtt cggctactat tcaaatcttt gaaaaagcct tcactttgag tttgtttcac
    21601 ttcataccaa tggttctcta gaatcatccg agctagcttt tgtcgatgcc acaagcttcg
    21661 ccaatgatca agaaaaaagt gaatataaag caatatcata gctcctgaaa aagcgagact
    21721 tccaagcaga catactaaat cttttataga ccaaacgatt tcaatagtag aaagtgatag
    21781 cttagaaata tcaaattgtc taaactcttg aaaataaaag acgctcatcc aaccgatgaa
    21841 cagagcgagt agccagccaa gaacaaagtg ataaaccaag tgctgatctc ctgcgcgtat
    21901 gcgatgtcct ttgtacattt tgatggtttc cacctccaat cgaaaaagcc caataggatt
    21961 tcattaggca atttcaaaaa gttatgctag tcatttactg ctacgaggag gggagttgtt
    22021 tcgtttcaac caattgattt aaaaagcctt caatagcatc cggcatctcc acaccaattt
    22081 cttccaaaat ggattctgcc tgccgacata agttttcatt tacttcgaca actataaaga
    22141 aagactcttc ttctcctaac cagtctagta catcagagtc ttcttcactg tcgctatttt
    22201 ctccaagtaa accttgatgt ataatcagaa acattttttc atcagaggtt aatgctttaa
    22261 cgagttgttc ctcagtcagc tcgccttttc catattgatc gagacaatct aaaatatgtt
    22321 caacattaat ttccataatc ttcacctatt tctttctttc attttggttc gtaggatttc
    22381 cttgattcgg ctgccgcttc aagactaaat catccgcctt aatgaaccaa ttaacggtag
    22441 ttccttctcg aaaaacataa ttggcaacgg tatccacgat tggattgacc aactctacct
    22501 ctgcatcata agcaaactcg cgtaagggta cattagctgg gatactaact tgaatcattc
    22561 gcccttgtgc agaggatttt aaattatagg ttcgttcttt tacctcatta ccaattgttc
    22621 catcctcatt ttgcttccgt acttctcggc gtaatgcgga aaaacgcaac ttcccaaatg
    22681 ttaaacctga atctacgaca ataccttctg ttaatctcat cttcttttcc tcctatgctt
    22741 tcactaaatc atcaacatac aacacgtaat cagtaaaccc acgattctca atctcatagc
    22801 ctttggcaac caaatgggga ttcacggctt ttagcggttg gtcttgatca aaatcctttt
    22861 caccggcttc cgcagctact accacctcaa tatcatcggc ccgttgctta ctggaataca
    22921 aatgataact gcgaccaatc accgtagtat tccgtccata tccttctgtt ataatttctc
    22981 cttcaccggc ataacttaac tttccaaagg tttcagccat atctggcacg acaaatttca
    23041 attccatctt cttttcctct tttcattgtt agttttggat acaaaaatag cgcgactact
    23101 tttttaggag gcggtcgcgc acgttggttc ttaggattta cttgtatcat ctcatcacct
    23161 tagttccggc gattccagaa ataatagccc gccgtcgcaa aaatcaaagt aaaaccaatg
    23221 aacagtaaaa cattggaatt tttctccccg gtttgaggga aggtcttcac tgtgggacta
    23281 gattcttgag agccacttgg aacagttggt gtttccggtt gtttcggcgt acttagtatg
    23341 gttggaactt cttttggtgt taaggtttga gatttttctt tcaaatcttc attgtgtttt
    23401 ccattgatat tcccatcttt atcgtaattg atttccgcaa aagtaaactt ggttccttct
    23461 ggatattttc cggtatctac ttttttcgca agcacagttt tggtgaattc tttatcattt
    23521 acttcataat caatcttgcc agatttccaa atttctttgt ttgtaccatc cggtaataaa
    23581 gcatacagaa ttgtttcgaa agcttctttt gagccatcca gtacatcatg agtaatcgac
    23641 acatcatcaa acatatccac cacatcacca tgagtaaagg tctgagaacc gttttctagg
    23701 tgggcctttg tttggataga aacatggtgt tccactgtac aactgacagt ttgggcttgg
    23761 ttattcaagc taacatcctt cgctactggc ttatcgcctg cttcaaaagc ttccttattt
    23821 tcatacacat agttgaataa gacaatagtt ttatttaaaa cttgttcggc ggttaattta
    23881 tgctgaattt tccaagttcc tgttttctct ttgttcgcca acgttgcgct tgtttctgca
    23941 atcaccactg gtttggcttt ttcatctttt tcttgggcag cttttgtcgc atccacatca
    24001 atggcttgcg ctaccacata ccagtcttct ttgatttcat gtagattgta actcaattta
    24061 tcaactagct cggtagaatc cagagaagtt aattctttgt ttccgtcttt ccaagtcgca
    24121 agagaagtta aactatcttc ttcctcaggc aaatcataga gcattaaacg gttcaaacta
    24181 acagaaaatg ctttgttcgt cagcttctcg taaggaacgg tcaccgtttt aattggttgt
    24241 ttttggtctt gttcggtaat ggtaaagaca tactcactct tcacaaagtc ttctttgttt
    24301 tccttaaaag tagaacggat ttctaatggc gtaatctttt gaaaaccttc tggggcttct
    24361 acttcttcaa gtaaataatc cccataagga agattttcaa acttgccata accatcaaac
    24421 cctaattgct cgttataagc tgtggtcgct tcatcttcgg cacctgtgat ttcattggtc
    24481 ccttccaatg gcgacacttt aaaggtcaga tcattgaagc cagtttcggt agtgcccgca
    24541 gctgatccag caaatttaaa gaaatcaaag ccaaagcgaa tcacttgttc ttttgccgta
    24601 acatttcggg taatcacggc atttttttcg ctatcatcca cctttttgat ggatacagga
    24661 tactttgttt catcaaggct atatccttcg ggcgcttttg tttcttgcca ataatactcg
    24721 ttgatagcta aatgtttaac ggcaacttgg ttcttttcgt ctaaagtcaa agtgaccgtt
    24781 tcatcagaac ctttcgttcc cttcgctaac tctggtttaa aagcctcact ccatttaaca
    24841 gcttttccat ccttagcagt aaacaaggtg tattcagcac cctcaaagac tgccttacct
    24901 tgtgctttat ccccagtatc tttgtcttct ttcgtcaaag tggtttcgcc agtgacttct
    24961 tgattttgcc cttttacgtt actggtaaca ggggctacgg tttgattggc atacttcaat
    25021 tcgactttta caggtttgaa cgtattcacg aacccatgac tagctttagt ttcggtcaca
    25081 taataggttc ccaattccaa cgcattagca atctcttttg gtgtttccgc atgaccgttt
    25141 tcatccgtgg tcatttcttg aacgatttca ccggttggac tgtctttgcg aatggcaaac
    25201 gtattaccag ctaaggaata attgtcattc caaagatcac tccctgtttc aactccgctt
    25261 ttatccagaa tgatttgccc tttttcccgt gcgttttttg aagtcacgta aatggtttcg
    25321 cctgctttaa tggtggtagt catgggggtg gtatcaatcg tataaggcgc cggtactgat
    25381 ttttcggtaa tggttacttt tgtaccatga ggaatctcat ccaatgtagc aatgccttct
    25441 ttgtcagtag tcacgtcttt tgcaggtaaa gtttttccaa agtctaaatg gaaaaccgtt
    25501 cctggtacaa catcacccga ttctttatcg acttttttga tctttaaaga accctccgtt
    25561 tccacatcaa ttttgattgt gtaggtatta ggtttatcaa tcgccccggc cattaaagtc
    25621 tgttggccag cctttttata ggcgacaggt gttccggtac cagcagattt cttaagcgtc
    25681 agcacaccac tttcttttga attggcattt ggggtgatta tcaactgatt accattcaca
    25741 cgataatcaa tgtttgcggc attttccaca acctcatcga actctgacaa attcagatta
    25801 ttcgtatccg tcacagtagt cgattgccct aatactgttt tagccgtact attatggaag
    25861 gttggtttct tctgataatc gtcaatggct tgatttattt ttgcctcaat ggctgcgata
    25921 tttacggcac taccatctga tcgttttatt gagtgaaggg tatacccatt aacttcttgc
    25981 cagatcatct tctgtgccac aatatgagta tctgcatctg tacctacttt tttccataaa
    26041 acggaaacta atttggcttt atcggacata tctggtaacg ggtttttctc atagccagga
    26101 gtgaactcat tgtaaatcgg gactcctggt tcaatacaga aaatatcttg tttaaagccg
    26161 ttgtacactg cataatgcgg aacagtgact tctgacattg gacgaccatc actaaaagtt
    26221 ccttcaacag catattgatg tgctaaatct agttctacgg ttaccgtttg tggatgggta
    26281 atttcatcag caaaagtaat cgtaggagca attgctccaa caattgcttg aacagccaaa
    26341 ctcaaaatag ttaatacaca taatttcttt ttttgttttt tatacatgaa atgttctcct
    26401 ttcaaattaa gcacaaaaaa ataa
//
LOCUS       DAYYAG010000030        24402 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000030 DAYYAG010000000
VERSION     DAYYAG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.030"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..962)
                     /locus_tag="ACHARQ_001071"
     CDS             complement(114..962)
                     /locus_tag="ACHARQ_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8409506.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1186..3126)
                     /gene="thrS"
                     /locus_tag="ACHARQ_001072"
     CDS             complement(1186..3126)
                     /gene="thrS"
                     /locus_tag="ACHARQ_001072"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8409507.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3463..3642)
                     /locus_tag="ACHARQ_001073"
     CDS             complement(3463..3642)
                     /locus_tag="ACHARQ_001073"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8409508.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3689..5512)
                     /locus_tag="ACHARQ_001074"
     CDS             complement(3689..5512)
                     /locus_tag="ACHARQ_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8409509.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5790..8069
                     /gene="helD"
                     /locus_tag="ACHARQ_001075"
     CDS             5790..8069
                     /gene="helD"
                     /locus_tag="ACHARQ_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8409510.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8162..8809)
                     /locus_tag="ACHARQ_001076"
     CDS             complement(8162..8809)
                     /locus_tag="ACHARQ_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409511.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8988..9707)
                     /locus_tag="ACHARQ_001077"
     CDS             complement(8988..9707)
                     /locus_tag="ACHARQ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8409512.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9838..10989)
                     /locus_tag="ACHARQ_001078"
     CDS             complement(9838..10989)
                     /locus_tag="ACHARQ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8409513.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11206..11946)
                     /locus_tag="ACHARQ_001079"
     CDS             complement(11206..11946)
                     /locus_tag="ACHARQ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8409514.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11950..12273)
                     /gene="rsfS"
                     /locus_tag="ACHARQ_001080"
     CDS             complement(11950..12273)
                     /gene="rsfS"
                     /locus_tag="ACHARQ_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8409515.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12330..12923)
                     /gene="yqeK"
                     /locus_tag="ACHARQ_001081"
     CDS             complement(12330..12923)
                     /gene="yqeK"
                     /locus_tag="ACHARQ_001081"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8409516.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12907..13557)
                     /locus_tag="ACHARQ_001082"
     CDS             complement(12907..13557)
                     /locus_tag="ACHARQ_001082"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8409517.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13567..13878)
                     /gene="yhbY"
                     /locus_tag="ACHARQ_001083"
     CDS             complement(13567..13878)
                     /gene="yhbY"
                     /locus_tag="ACHARQ_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8409518.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13894..15003)
                     /gene="yqeH"
                     /locus_tag="ACHARQ_001084"
     CDS             complement(13894..15003)
                     /gene="yqeH"
                     /locus_tag="ACHARQ_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8409519.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15000..15527)
                     /locus_tag="ACHARQ_001085"
     CDS             complement(15000..15527)
                     /locus_tag="ACHARQ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8409520.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15707..16492)
                     /locus_tag="ACHARQ_001086"
     CDS             complement(15707..16492)
                     /locus_tag="ACHARQ_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8409521.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16485..17354)
                     /gene="accD"
                     /locus_tag="ACHARQ_001087"
     CDS             complement(16485..17354)
                     /gene="accD"
                     /locus_tag="ACHARQ_001087"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8409522.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17355..18734)
                     /locus_tag="ACHARQ_001088"
     CDS             complement(17355..18734)
                     /locus_tag="ACHARQ_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8409523.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18742..19161)
                     /gene="fabZ"
                     /locus_tag="ACHARQ_001089"
     CDS             complement(18742..19161)
                     /gene="fabZ"
                     /locus_tag="ACHARQ_001089"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8409524.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19186..19662)
                     /gene="accB"
                     /locus_tag="ACHARQ_001090"
     CDS             complement(19186..19662)
                     /gene="accB"
                     /locus_tag="ACHARQ_001090"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8409525.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19665..20900)
                     /gene="fabF"
                     /locus_tag="ACHARQ_001091"
     CDS             complement(19665..20900)
                     /gene="fabF"
                     /locus_tag="ACHARQ_001091"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8409526.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20919..21656)
                     /gene="fabG"
                     /locus_tag="ACHARQ_001092"
     CDS             complement(20919..21656)
                     /gene="fabG"
                     /locus_tag="ACHARQ_001092"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8409527.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21668..22585)
                     /gene="fabD"
                     /locus_tag="ACHARQ_001093"
     CDS             complement(21668..22585)
                     /gene="fabD"
                     /locus_tag="ACHARQ_001093"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8409528.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22692..22916)
                     /locus_tag="ACHARQ_001094"
     CDS             complement(22692..22916)
                     /locus_tag="ACHARQ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8409529.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22969..23931)
                     /locus_tag="ACHARQ_001095"
     CDS             complement(22969..23931)
                     /locus_tag="ACHARQ_001095"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8409530.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23941..24381)
                     /locus_tag="ACHARQ_001096"
     CDS             complement(23941..24381)
                     /locus_tag="ACHARQ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8409531.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6676 a         5428 c         4255 g         8043 t
ORIGIN      
        1 ttatttttga aaattatgtt ttcttaagtc gtgaaacact ttgatggatc atttatttgt
       61 ggaaaactac gttttgttat tctaaaaaac ttatataaaa aatagtaagg tttttatttc
      121 gaatccataa aatattcttg gattgcttct cgtgtttttt tcactttttc gctaagcttt
      181 tcgtttttag ccatatagaa ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt
      241 gaattagttg ccgcacttcg attcgtgatt tcttttggtc tagatgtttg tacgagtaca
      301 tcggctatct tgctaagcgg gttatttcca aattgcgtca acgcaatgat ttcgatattc
      361 atgcttttcg ccagctcggc taatgcgact acatctgcac ttctacctga gtttgaaaca
      421 agccaaagca ctttcttttt ctcattgctg acaagttgcg ggagcaagac gtggatatct
      481 ttttcaaaaa tgattggctt accgacacga gaccattttt gcagaatatc ttctactact
      541 aaagaagagg cgccaacccc atataaaaaa acagtctctg ccgattccaa acactctacg
      601 actttttgga tcgtttcttc ttctagaata tctactgttt cttttatcgc tacttgtgca
      661 ttgttaccaa gtttgttttt gatagagtaa aaggactcat ttgcttctat atcactaaaa
      721 ccttcatcga ttccagtctg tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt
      781 tgaaaaccga tttttttgga aaaacgtaca actgtcggcg cactcaaccc ggaagcttct
      841 gctaattcat tagctgtcat tgtagggacc ttctcaatat taccgataat gtagttggcc
      901 aacttttttt ctgctgaaga ataatcttcc atcatacttt caatacgact aattacgctc
      961 ataagttcct ccaccctttt aatgatagtc tatgtaagta taccatatca gacccttatt
     1021 cacggtcgta tactggataa aaaacaaaaa aacaatccgc ccaacagaat atcttatccc
     1081 atcaatccca cagattctat tgagcatctg tgtctttgag gaatacctgt tatctattgt
     1141 tcggattgct ttctatctac tactttttta caggaaattt acctattagt tttctctgct
     1201 gtaatttttg acttctgctt ccatagcatc aataaagata ttcaaatctt ctacagctgt
     1261 ttctttactt ccataacgac gtacgttcac tgttgcatct tccaattcct tgtctccaac
     1321 aactaattgg taagggatct tttgtgtttg agaggcacgt atcttatagc ccattttttc
     1381 attacgatca tcaacttcta cacgcaaccc cttcatttgc aaacgttctt tgatttcata
     1441 agcataatcg ccatgtgcat caacagaaac agggatgatt gttgcttgta taggtgccaa
     1501 ccatgttgga aaagcgcctt tgtatacttc tgttaaataa gcgacaaaac gttccattgt
     1561 agaaacaatt ccgcggtgga taactactgg acgatgcgta ttttccccgt cttctccaac
     1621 atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat tgtatagttg ataatgtttc
     1681 ttctgtacca agagctgttt tgacttgaac atctagtttt ggtccgtaaa aggctgcctc
     1741 gccttctgct tcaaagtaat ctagttctaa ttcatccata gcggctttca gcattgcttg
     1801 ggctttttcc cacattgcgt catcatcaaa atatttatct gtattgtttg gatcgcgata
     1861 gctcaagcgg aaacgataat ctgtaatatt gaagtcagca tatactgcca ccatcaattc
     1921 aagcgtacgt ttaaattcat ctttgatttg atcaggacga acaaatgtat gaccatcatt
     1981 caacgtcatt tctcgtacac gttgtaaacc tgacaaagca cctgattttt catagcgatg
     2041 catcattcct aattcagcga tacgaattgg taattcacga taagaatgga tcgtgttttt
     2101 gtagaccatc atgtgatgag ggcagttcat tgggcgaaga acaagcattt cgccatctcc
     2161 catatccatt ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag atgttttgta
     2221 caactcaaca tctcccatta ttggtgtata tacatgttgg taacctagac taacttcttt
     2281 gtctacgata taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg
     2341 caaaccagaa cctacttctg gggaaaccat gaacaagtca agttcttttc ctagtttacg
     2401 atggtctctt tcttttgctt cttcacggag acggatatat tctttcaaag ctttcttgtc
     2461 aaaaaaggct gtcccgtata cacgttgcat catttgattg tttgagtttc cacgccaata
     2521 agcacctgcc actgataata atttaaatac ttgtatacgg ccagtggatg gaacatgtgg
     2581 tccacgacac aaatcgatga attcgccttg ttgataagca gtaatcactt cttcttctgg
     2641 caattcacta atcagttcta ctttgtacgg atcacttgca aaaatttcta atgcttcctg
     2701 ttttgaaaga acacggcgtt cgatcggcag attttctttg acgattttca tcatttctgc
     2761 ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt ccattatctg tatcgtagta
     2821 aaaccctgaa tcgatagctg gtccaacgcc aaagtggata tttggataga ggcgacgcat
     2881 ggcttgggcc atcaaatgag ctgctgaatg acggactaag ccaagtgcat cttcatggtc
     2941 tggtgtgatg atctcaatac ttgcatcttc ctcgattgga cgagtcaaat caacgagttt
     3001 tccgttcact ttaccagcta aagctttttt tgctaagctt tttgaaatgc tttcagcaat
     3061 tgccaatgtt gttgttccag actcaaattc tttgactgca ccatctggaa aagtaatttt
     3121 tatcataata gtttcctcct tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca
     3181 aaaaaatcct agcatctctt gaaaagagat actaggacga aaagttcgtg gttccaccta
     3241 atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac
     3301 aagggtttcg aaagtggtca tcctttggtt ttctcctgga aaagtttcag cctagctttt
     3361 cctctcttat cgaagactct caaattcagt tggctttctc atcaatcaat tatgtgttta
     3421 tttaaccact aataaataga aaaatcaagt gggaaactaa acttattttt tccattcccc
     3481 atttacaccg tatgttcctt ttttcatctc attgatgatg acatggatgt gttcttttgg
     3541 tgcctgcgtg tttttatgaa cagcttccgt gatatctttc atcatatttt ctaattgttc
     3601 cggactgcga ccttctacta attcgacatg tacgaatggc ataatgctct ctcctctttc
     3661 tgatcgttct tttttcaact cgattctatc atactatgtt tgtcttgggt actgaatcag
     3721 gaaaaatggc tggatctttt tttgctctca aggcactcaa aatagagacc gtatccacga
     3781 cttcttgcaa aacagctccg aataatgcgg ggattagtcc cgtacttgca attaacatca
     3841 aaaaaataca aataaagatg ccaatcagta cagactgttt cgctattctc atcgtatctc
     3901 ttgaaatcac tactgctttt gctacacgca agagatcatc tttcaagatg accgcatctg
     3961 cactttcact agcagctgta gaaccatgag cccccattgc aataccgata tctgcagcag
     4021 caagtgctgg tgcgtcattc acgccgtctc ctaccatcgc aacaggtctt tgatctttag
     4081 ggatactttc taaaatggtc agtttatctt gaggcaagct acgggcatgt acagaatcta
     4141 attggacttt ttctgcaatc gtttgagcaa tggcagatcc atccccggtg atcatcatca
     4201 aatttttcac atcaagtttt cttagctcct tgattgtctt tgctgcttct gggcgaatct
     4261 gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat tgttacatag attgctgtat
     4321 tggacgtttc tatttcttca gctgaaacaa aagaggcacg tccaatcttt acttcatgat
     4381 tattgactac tgcttggatt ccttgaccgg tcacttcttt cagttgacta acaggataca
     4441 gttggctgtt tttcacgttg ttcactaacg aacgagctaa gatatggcta gattcctgtt
     4501 ctgcactagc tgcatacaga agtagctctt ctttttttat tccattgact ggctgaatcg
     4561 cctctacttc caatatccct ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg
     4621 tacgtgcgag cttttcaatc gttgttccag tttttacaac gatcccgttt ttacttgaac
     4681 ggctcattcc tgcaactaaa gcaatgggcg cagctagaat cagtggacaa ggtgatgcca
     4741 ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa ataccaccga
     4801 tcaaataagc aatcaacgtg aaagggactg cataacgatc agctaaacgg acaaatcggg
     4861 caggcttttc ttttgattct tcgactagtt taataatcat ttgatactgg ctgtctgcgg
     4921 cacgtttttc agcgatcatt tggatcgatc cgtctccatt taaagaccct gacataatct
     4981 gatctccagg tttcttggtt tccgggcggg actcacctgt caaagaagat tcatcgaccg
     5041 aagattctcc tttggtcact cgaccatctg cgggtacaag ttcgccaggc ttgactagta
     5101 actggtcatc aatatttatt tcatcgatta ctatatcctg caattttcct tctttgaatc
     5161 gatgggcaat tcttggggtg ttatctaaca aagactgcag ttctttaccc gctcgtttat
     5221 tcgcgtaatc ctctagacta tctccgcccg taagcatgat caatatcatc aaactagccc
     5281 agtattcttt gacggctaat gttgcaagga ttgcggtaat cgctaataca tctacgccat
     5341 atcttcctga acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg agaatccctc
     5401 cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat tcacttaata
     5461 aagctatccc accaataagc aaagtaagta aaaatttttt gaaatgtgtc attgtttttt
     5521 ctcctctttt atgtgactta gagaggttag taataaattt ccagctgcct ctttcatctt
     5581 aagcatagtc cttgactagc actttttaaa acaataggcc ttaattgaaa acaactatca
     5641 ctatcagcgt ttccaaaatt catcagttct acaaacaaaa atattcagca aggattactt
     5701 tttcgattca tcagttaaaa agtacctttt ttcgaaaaac tatgctatac ttttaattcg
     5761 ttatttacgg cggaaggagc aaacatgcga tgaatgagaa acaactagaa caaacgcacg
     5821 tggatgaaac gatcgaacta atccagatcg aacaaaatat gctggataca aaacaacaag
     5881 aattgactca ggaaatgaat cattcgacaa aagacacagc taatcacaag attcgagctg
     5941 gttccaatga agctttttat gaatctgctg ttgaatatcg ccaacatgag caggaattgc
     6001 ttttgaaata ccatactctt gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga
     6061 aagatagtcc ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat
     6121 atctaggtat cgcttcttta agagatctgg cagaagaaac aatcgttatt gactggcgcg
     6181 caccaattgc caatctttat tatgaaggtg agattggtcc cgcattttat gaaactgaaa
     6241 cagatagaat agatgtcgat ctgcagttga aacgacaatt taaaatccaa gaaggacgta
     6301 ttttatctat ggtagatacg tcggaagtaa tcaatgatga ctttttgcta gagatattgg
     6361 atgaatcatc aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg
     6421 caatcattcg ggacacaaca agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa
     6481 agacatccgc tttgcttcaa cgtattgctt ttctgttgta tcataatcgc aagtggctcg
     6541 aggctgaaaa cgttcttcta ttttcaccca accatttgtt ttctgattac atctcgactg
     6601 tattgccttc attaggtgaa agcggcgtgc ctacacagac attcaaaaat tatttggtgc
     6661 agctgctgcc agagttttct ttactagatg aacagcaaca agaaagtggt tttttgctag
     6721 gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac cttagtcgat cagattgatc
     6781 gctatatcca aggcatcaca aactacggtc ccttgttccg tgatatgaaa ataaatggcc
     6841 gtacgatcct atcaaaggaa tcgattcgcc aatggtataa agaaacaaat gaacaattac
     6901 ctttacatca tagactttct ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc
     6961 aaaaagacga aacacgtcag caatgggtca aagatctggc ggaagaacag ctccaagaac
     7021 tatacgcaac agatccaaat cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa
     7081 aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt
     7141 atcaatttgt caatataacg aaacaatatc tccactttct acaaactgtg ccactttctc
     7201 ttctaacacg acataaaatc agcgagacca ggtggcagga acatttgaca actgttcgta
     7261 cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt actattcttt ttactgatgc
     7321 gccgaacata tccagtgacg gttacacaca aagctcgcta tatctttatt gatgaaatgc
     7381 aggatttcgc accatcacaa gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga
     7441 ctttctgtgg agatctaaat caaaatgtct ttggaaatga aacgatcgtt ggatcattgg
     7501 aagacctttt cgtggaccgt gaagtcactc gtttccaatt aacgacaagc tatcgctcca
     7561 caaaagaaat cactgatttt gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa
     7621 cagcaagaaa aggagacctt cctttgctca tccaaggaac aacagatgat atcaaattga
     7681 attggttagc agataccatt cgaacgactc agggaaaagc ccggtattgg cgtacagcaa
     7741 tcatagctaa aacacaagaa gaatgcgaag ctctttatgc tcaactgcca gacgagttga
     7801 aacaacaagt ccagctgatt ttaaatgaga gtgactttat gaagcgttcc attattttac
     7861 ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg
     7921 cagaacattt ttcttctcag caagacaaat tgatccttta caccatgtgt acacgtgcga
     7981 tgcatgaatt gacccttttt acctccaata atgaaagtcc tttgttaaac aatatggata
     8041 tttcatctta tgagaaaaaa gtactgtaag taaaaagaca actgctatca ttaatgttag
     8101 cagttgtctt tttatttaca ataataattg cagaaaaagt aattcaatca tttaaggtca
     8161 tttattctat atataaatcc aaatctgttt caggatattt taatttgaca agcgaaataa
     8221 tcattttcag ttcttccatg ttttctggat attctgggtc aaataccttg ctatgacggc
     8281 caatcccgcc attgtcgatc aatcggtaaa ttcgattcca ttcaatgaat tggtcaattg
     8341 attggaaatt gctgattgga tagccgccac aatccaaaat ctctgataca cgaaatgcta
     8401 ggtatgcact atcgatcact ttattttttt tcgtgtctat cttctcattt ctttcgataa
     8461 gaattttttc aggacgatgt ttcaagctcc actctaatag tagaaagaaa gcctccatcc
     8521 gatactcttc ttcaaataac cgaaaggctg aatcagaacg acgaaaaagc agcggaatca
     8581 gataattttg tctataaatc agcattggtg cgcggtccac catacactct aaataacggt
     8641 tcagataccc tgtaccgaca tagtttccta atcgatagcc ttcaacgccg aatcttgcgt
     8701 atttttgcgc taattttgaa caatcagtta cgttcacagc taactcttct actttgcggt
     8761 aaccgcttaa gatacgttca aaaggttcta aagtgatttt tgcttccata ttcttccccg
     8821 tccatcttcc acatacttca tatcatttca catgatactt atgctctcat cataccacag
     8881 gttgaattag aaaagaaaat aaatcacaca aaaaagcaag acaatctgtt agtgagattg
     8941 tcttgctttt cgaaaaagac tgaatacttt tcgttaaaac tggttgctta gtcttctaat
     9001 gccacattat ggaacacatg ctgtacatct tctagatctt ccagtgcatc aatcattttt
     9061 tcaaatgttt cttgatcttc tggagatagt gttacctcgt tttgagggat catttggatc
     9121 tcagcgattg aaaaatcagt gattcctttt tcttttaatg cttcttgtat actatggaaa
     9181 tccgctactt caccgtaaac gatgatttgt ccttcttctt ccattacatc gcgaacatcg
     9241 atgtcttttt ccattaggta ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag
     9301 attgctgtat tgtcaaacat gtaagaaacc gctccggaaa cacccatgtt cccgccattt
     9361 ttcccaaatg ctgcccgtac gtctgctgct gttcgattca cattgttcgt caatgtatca
     9421 acaatcacca ttgagccgct tggtccaaat ccttcatagc gcaattcaga gtatgtttca
     9481 tctcctgcac cttttgcttt ttcgattgct cggtcaataa tatgttttgg tacattataa
     9541 gttttagcgc gatcaatgac gaaacgtagt ttttggtttg catgcggatc aggatcacct
     9601 gattttgctg ccgcgtagat ttcaatccca aattttgcat aaatccggct attattagca
     9661 tctttagcag ttttctttgc tacgatattt gcccatttac gtcccatatt ttcacttcgc
     9721 tttctgattt gatagtttca taacgcaaaa acgtcctgtt ccattatact tgtgaatcac
     9781 gattctgtga agcttttttt ctttcttcct tcattcttct catttttagt caattatcta
     9841 tactcggatt gggatttgcc cgaaattctg ttccggaata ttgcctccca attgatagat
     9901 ttgatcactt ctaactaaca gttccgcttc ttgtgcctct ttttttccga ttcgggaata
     9961 aatagaaatc tctttttttt cttttaagaa tcttcttcca gcatcggtaa atccaagtag
    10021 tcgaagataa tttttatttt gttggacagg aatctcttgc tttttgatat tcagcaagac
    10081 ataacaagat aatcgttgga tccgtgtcca tgtatatctt tttgttttta cctttgacac
    10141 aaaatcctca aaactatcag cagttgcagc tgctgttttc atgcgcgatt ctattccttc
    10201 ggtcatctga tagatatctg tcagttccag caaagtagaa gataacaacc gataacgcaa
    10261 atacggccaa taagattccc aagtctgcag cgaacaagtt gaaagatctt cttctgtctg
    10321 aggcggcaca agtccagcta ttctttttcc ttcttctaac gcatggcgaa tggctgttgc
    10381 actggctatc ctctgatctg tgatgatttt ttcatgatag cctgcacttt tacgtcggat
    10441 cggatgtaat tccatagggt ggacatactt ggcattttcc tttgcataac ttaatcccaa
    10501 gatatgattt ggcgaagaaa aatcaagcgt aatatcggga tacagactgc gaaaaacctc
    10561 agtcattttt tgcggataac tatacgtttg atcagacagt tcttgaaatg cctcttctat
    10621 cctatcttga ttctcttgga caaagcgtcc gaaactgctg taatcaaacg aagtctcttc
    10681 ttctgttcca aaacacaaac tgtcacagtc cagtgtttgc aagattttta ctgcaccttt
    10741 agcaaaatag tcagctgact gaaccgccca ttcgacggga agctccacta ctaaatccac
    10801 accattttgt aaagcttcat tcgcgcgatg ccatttatcg atgatagccg gttcgcctct
    10861 ttgcaaaaaa ttcccgctca ttacagcaat gacgacctct gctcctgtta tttttcgtgc
    10921 catttccaca tgatagcgat gtccattgtg aaaaggattg tactctacga taatcccgca
    10981 gcttttcatg cgcatcctcc ttcatagtta tcgataaaaa agaaatagag acaagtaaaa
    11041 aaaggcaaaa atcggaaaaa atacccgaac tatataaact attctcgcaa acacaatact
    11101 tctgcgacga aaagctgcac tgcaagagca atgcctatat cgttcggata tataatttat
    11161 gttgaagatt tattcttttc ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc
    11221 tacaaagaac cagcgctttg atttctctga tgctgcttcg tctgtaaaat cagaaaatgc
    11281 ttcgactttc gaaaagccgc tgttttccag cattcttaaa taactttcca atggataggt
    11341 acgctcttga tgtagttcat cttcacgaat aaatcgacca gacccttctt gttcttcaac
    11401 gaaaaaggta agaaaatgtt cgatgctgtg tttctcgctt cctggataac tatcccacaa
    11461 aaaagcaaaa ttatcccctt gataatgata gctatattct ggaaaaacct catctacttg
    11521 ataggtggag tgtacgtcaa ataaaaatcg gccattttct tctaacgctt gatagacttc
    11581 atcaaacact tgctgtacct cttgtctatt tttcatatag cacagcgaat cagaaaaaca
    11641 ggtaattgcc tgatattgtc cgatttctga taaatcgagc atattgcctt ccacaaattg
    11701 gatcgatgcg tcttcttcat atgctcgctg gctggcaatc atcaacatct cttctgaaag
    11761 gtctaaccct gtcactttat atccatcttt tgcaaaggca accgctaatg ctccagtacc
    11821 gcatgccagt tccaaaatat tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa
    11881 ccattgctga taaagtgttt cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata
    11941 agccatatgt taatccaacc attcactaag atcgactagc ggagcatctg accatagttt
    12001 ttccagatta tagaaatttc gttctggcgc atggaaaaca tgtacgatca catctccaag
    12061 atcgatcaat acccattttc cgccttcttt tccttcaata cgttttactt catatttgtt
    12121 ttcttcttct ttatcaatga tttcttctgt aattgcgttg atttgacgtt cgctatttgc
    12181 tgaacagatc atgaaataat ctgccaacaa ggaaacattt cgaacgtcta aagcgataat
    12241 gtcttctgcc cgttttgaat ctgctgcttc aatcgcaatt tttaattgtt ctttactatc
    12301 tatgatgatt tcctcctaat atgagcagct tatttttttg ctacccagta attatatgtt
    12361 tcgattgttt ttggataaat ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt
    12421 gtttcaaatg ctacagcttc gtctagatca gcccatgcaa tggctcgtgc atcttgtacc
    12481 ccaggaaaat ctcgacctgg ttcaatatag tctgctacat aaatgatttt gtcaaggaga
    12541 ctcatcttag cagccccagt cgtatgcacc cggattgcat gaagaatctc tgcatcggtg
    12601 attcctaatt cccgctctac aaacgaagcg ccaacaagac catgccagat tgcattatta
    12661 taattcaata actccggatc aaaaccgtct cttacgatta ccattttgaa ctcctcatct
    12721 ggtcgttctt ttgcataatc atgtgtcaat gcagcaatac tagctttttc aggagaagca
    12781 ccgtattttt tggcaagagc cacggctgtt tcttctactc caagcacatg cttgaagcgt
    12841 tgttcgctca ttctcatctg tattttttgc atcaggtttt ctctagaata agcagtatat
    12901 tcattactat agtccatatt catacaaccc ttcattttga atataatcaa ttactttatc
    12961 tggaacaagg tagcggatag aacagccttc tttgatcttt tgacgtattt tcgtggaact
    13021 gatgtcgatc tcaggtacat ctacccaaat tacaggatat ggagtatccg ttgtgtatcc
    13081 tggtcgacgt atacccacaa agttgaccat agaagtcagc tcatcgattt tataccattt
    13141 tggcaaatat tctaccatat ccccaccgat aataaagtaa taatctgtat ccggattgtt
    13201 ttgcgtcaat tccttcatag tatcataggt gtaacttttc ccaccacgag ctaattcaat
    13261 cgtttcaatc tgcaaaaaag gattgtcttc caccgctaat tccaacatgt tgagtcgatg
    13321 ttttgcatca atcgtctgtt tctcatcaac atgaggtggc tgataagaag gcatcaaaaa
    13381 tacgcggtca agacctaaat tccggccagc ttgttcagcc atcactaaat gtgcaaggtg
    13441 aacgggattg aatgttccgc ccaagatccc tacttgcttt ttcttctcta agaattgagg
    13501 catttcttca ggaaataatt gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt
    13561 cactccttat attgctttta cttcttttga tagcctttga tacttttctt ttgtggaagg
    13621 tttgtataat acaataacac gtccaatgat ttggacgatt tcgcaatcta tttctttttc
    13681 taatacttga gctgcttctt ctgcaatctc atctgtattt tgaagtaacg atactttgat
    13741 cagttctctt ttttctaaag cttcttcaat ttgaacgatc attgctgaat tgatcccacc
    13801 ttttccgatt tgaaaaatcg gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg
    13861 ttttcctctt aaactcataa ttttccttct tccttaaatc aatgctttgc gtttcaatac
    13921 atctacaccc tttggcgccc agcccgcaat tactactggc gctgttacag tgatccagcc
    13981 tagaccggca aaaacgatgt ctgttttttc tttcacagaa aactcaaaac gaacaagctc
    14041 cggaaatgcc tcgacttcat caggacgagg aggctgtaat aatccaccta catgtttttg
    14101 ataaaattcg tctgcttttt ccagtttcgt tcgatgaata tttacatcat ttgacacata
    14161 agcaataaat gaagcacgat tgccgctgat aaaatcaaat cttgcaagtc cgcccaagaa
    14221 aagtgtttgc ccttcattca gctgataaac tttaggtttg atttctttta acggtgcaat
    14281 gatccgcaaa tcttttttac ctaagtagtg cgccatttga tggcgatgga taattcccgg
    14341 tgtatcaatc aaaaaatgcc cgtcatctaa aggaatctcg attttatcca aggtcgttcc
    14401 tggaaactgc gaagttgtga ttacatcttg tactcccgct gtatttttga tgatttgatt
    14461 gataagcgtc gattttccga catttgttac tccgacgata tagacatccc gtccatcgcg
    14521 gtattcttcg attttttcta atagttccgt catctcatga gatttcttag cactagttaa
    14581 taatacatct actggacgaa ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa
    14641 tttagttttt ttcaatgatt taggtaatat atccactttg ttcccaacta aaagtactgg
    14701 attgtcaccg acaaaacggt gtagtcctgg gattagtgaa ccattgaagt caaaaatatc
    14761 caccacattg acgatcaatg catctttttg ccctaattca ttcagtaagc gcaaaaagtc
    14821 gtcatccgtc aatgagacat cttgggtctc attataatgg cgtaatctga aacaacgctg
    14881 acaatagact tccccggaag ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa
    14941 ttcaccgggt ttgtttgttt gtataacggc accacagccg atacattgta attcttctgt
    15001 cattcgattc cacctcgcca gcccatatca gggtgttttg ctaatagata ggccataatt
    15061 cttttttccc aaaaacgatt aaattgtgtc ttccaactat ctgttgcgat aatgggtttt
    15121 acaaggatgc tacgtatccc tgcacgattg gctccacgta tatcagtcat aatctgatcg
    15181 cctaccatta ctatctcatc atctgctaaa tccaattgtt tctttgctat gttgatccct
    15241 cttgctaaag gtttcattgc tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc
    15301 gcgacacgtt ttgaattatt gtttgaaaca acaacaacag ggattcctgc atttttcatt
    15361 tctaaaatcc aagtaagcaa ctcttctgtt ccatctggat tattccatgc aatcaacgta
    15421 ttgtctaaat cggtcaatac tgctttgatg cctagattct ttaattgggc aggtgtaatc
    15481 tgatagatgg cttctaccat ccatgttggt ttgtaatttg aaaacatgct gtctcctcat
    15541 ctgcaaaaaa tgaaggagca cataaagtac tcctcaaccc cgtattatac tgcatatcag
    15601 caaaaaaaac tacctctata ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga
    15661 ccaaaaataa agattgatca cagaaaaatt tagaaacaaa aaacaactaa tattggcgga
    15721 acctcttgta tcgtttttcc agtaatttat gctgatctaa tcgaacaagt tccgtcaatc
    15781 tttcaatcaa cgcttttttc ataatgtgtg cgactttata tcgtggcaac tgctcgccgt
    15841 tgaacgtttc aggaataact cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa
    15901 ttttcatcaa ctcagctgct tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa
    15961 atccttctgg agaaagcaca gcataaatcg tttcttccag catccagact tcatccgcta
    16021 aacctagagc aagggcaccg ccacttccac cttcaccaat gatgatggcg atgatcggta
    16081 cctttaaatc agacatttcg agcagatttc tggcaatagc ctctccttcg ccgcgctctt
    16141 ctgctcctac accgcaataa gcacctgctg tattgatcaa ggtaatcacc ggtctattga
    16201 acttttccgc ttgtttcatc agtcgtaaag cttttcgata gccttcagga tggggagaac
    16261 caaaatttct gcggatattt tcctgcagat cccgtccttt ttgaataccg atcactgtta
    16321 ccggtttgcc atctaatcga gcgattccac caacaatcgc ttcgtcatct gcaaataaac
    16381 gatcaccatg gcattcctcg aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc
    16441 atcgttcttt agctcttgct aactgaacga catcatgtgc tgtcttatcc acgatctatc
    16501 catcctttct gtgtatgcaa ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg
    16561 ggaacaatct gatcgacaaa accatgagat aataagaatt cagccttttg gaaatcttct
    16621 ggaagttcct gcttgatcgt ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt
    16681 tggggttctg ctagaataat atcgccttcc attgcaaaac tggcagttac tcctccggtc
    16741 gtcgggtccg ttaaaacagt tagataaaaa agaccttcat tgctatgccg tttgacagca
    16801 gcagagattt ttgccatctg cattagagaa aagattcctt cttgcatccg tgcgccacct
    16861 gaagctgtga acaagacgac tggaagactt tcctttgttg ctcgttcgaa taatctggtg
    16921 atcttttctc cgacaatcgt tcccatactt cccatgataa aattaggatc catcactcca
    16981 ataatgaagg gaatcccctg aatggttgct ttaccagtga ggactgcttc atgaagtcct
    17041 gttttatctt gcattttttt aattttttcc gtatattcgg gaaatgctag cggatctttg
    17101 gttacaagat ccgtatccca ttcttcaaaa cttttttcat caataatcaa agctagacgc
    17161 tgccatgcac cgattcgaaa agcgtatccg caatgagggc atactttttc ttcaccgatg
    17221 tctttcgtat aaaggatatg tttgcaattt ggacactttg cccacatgtt atcaggaaca
    17281 gatggcgagt tagcaggttt gctctctcta ttaggattga tccgaatata tttctttttc
    17341 ttaaacagag ccatctatgc gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat
    17401 tcttcttgta aaaatgctgt gctgtaatca cctgctatga ctgcaggatg actaatcaaa
    17461 tccatctgaa attctgcgtt cgtgatgatt ccttctgtga taaattcact taaggctcgc
    17521 tgcatcttca ttaatgcttc aaaacgcgta tttccatgga cgattatttt tgcaatcatt
    17581 gaatcataat atggcgggat cgtatatccc gggtaaactg cactgtctac tcgcagtccc
    17641 atcccacctg ctggaagcag taagttttgg atcttccctg gagaaggagc aaaatggaaa
    17701 gcaggatttt ctgcattaat ccggcattcg attgcgtgtc cttggaatac tacttcttct
    17761 tgacacacat tcaaaggctc accggctgct atctccacct gtttttttac taaatcgatt
    17821 cctgtgacca tttctgttac tggatgttcc acttggattc gagtattcat ttccatgaaa
    17881 taaaaatcgc cttcttcatc catcagaaac tcgatcgttc cggcattttc ataattgact
    17941 gctttagctg ctcgaacagc agcgtctcct agtgcttctc tttttgtttg tgagatcact
    18001 acagaaggag attcttccag tactttttga ttgttgcgtt gtaaagaaca atctcgctcg
    18061 cctaaatgaa tgacatgacc aaatcgatca cctaagatct gaacttctat atggcgagca
    18121 ggataaatga ttttttccaa atacatatca tcatttccaa atgccgctgc tgcttcttgc
    18181 tgagctgaag aaaaatgctg tgggagctct tcttttgatt gaactttccg aatgcctttc
    18241 ccgccaccac ctgcagctgc tttcagcatc actggataac caattctgtc tgctatttcc
    18301 aatgcttctt ctactgtaga taatacacca gtacttcctg gaatgactgg gacatttgct
    18361 tcttgcatca attgacgcgc attgatctta tttcccattg catcgatcgt tgcactttta
    18421 ggaccaataa atgtgatatt gcactcttca cacatggagg caaatcggct attttcagat
    18481 aaaaaaccaa atcctggatg aattgcttcg gcttttgtca cgattgcagc gctcagtact
    18541 tcctgaacat tcagatatga atcaacggct tttgccgggc caatgcagat tgcttcatct
    18601 gctaactgtg tatgaagtgc ttcggcatct gcttctgaat atatcgctac ggtttggatc
    18661 cctagttcgc ggcatgcacg aatgatacgt acagcgattt caccgcggtt agctatcaat
    18721 actttagaaa acatatttca cctatccaat cataaaggtt aattccgctt cggctacttt
    18781 tttaccgtct actttagcga ttcctttgcc tattccggct gaagctttca ctttcaatat
    18841 ctcaacttca aggtataagg tatctcccgg tgtaactttc ttacggaatt ttgctttgtc
    18901 caatccgccg aaataagccg tctttccctt gaattgttca agagaaagta atgcaactgc
    18961 tcctgcttga gccattgctt caacaatcaa tacccctggc ataacaggtt cttctggaaa
    19021 atgtccttga aaaaagggct cattgatcgt tacatttttc ttagcaacga tccgttctcc
    19081 ttcaaccatt tcttccactc gatcaatcaa aagcattgga taacgatgtg gaatgatttc
    19141 ttttatttct tgtatgttca ttgttttccc tctttcctgc tttgcttatt tttgctgaat
    19201 cctaaagagt ggctgtccat attcgacaac tgactcgttt tctacaagga tttctgtgat
    19261 cactccgtcg gcctcactag ttatctcatt catcagtttc atcgcttcaa taatgcagat
    19321 aacttctcct ttggcaactt gatcacctac ttttttgaaa gctggtttgt cgggcgctgg
    19381 ttgcagataa acgattccca cgattggcga aacaacctcg attccatcaa atactgccga
    19441 ttctttttct ttcattattt ctggagcagt tgttgcttgt tcaggtatga cattgactgg
    19501 ctccgaaact ggcaacgttt cgtttgtttg aaccatattc atgcctcgac ttgttttatt
    19561 tttattcata tacaattcaa attgaccttc acgcaaatcg aattccgtta gcgaggattg
    19621 atcaaattga ctgactaatt ctctgatttc attgatatcc aaaattattc ctcccagcgt
    19681 ttcatgcaca gaaccgcatt atgtccgcca aaccctaatg aattactcat tgcataagta
    19741 aaacttgcag gtttgctttc gtttttgaca actgtcaagt caatctcagg atcttgtttt
    19801 tctacattga tcgtaggtgg gataaattga tgttctaatg ccagcaaagt tgccaaggct
    19861 tcgatcccgc cggctgctcc caataaatgt ccagtcatcg atttcgtgct gctgacgtaa
    19921 gtatctctga aattttcacc taatgcgatt tggattgctt ttgcttctgc ctggtcattg
    19981 gctggtgtac ttgttccgtg agcattgata tatccgactt gttccggctg tacaccagct
    20041 tcttccatcg caagcttcat tgctttagat gcaccagaac catctggagt aggagctgtc
    20101 atatggtaag cgtcacagtt tgcaccgtat ccaacgattt ctccatagat tttggcaccg
    20161 cgagcttgtg catgttctaa agattcaaga actagaacgc ctgctccttc acccatcaca
    20221 aaaccattcc gattttcgtc aaacggaatc gaaccacgat taggatcagt tgattctgag
    20281 agtgctgtta aagctgcaaa tccagcaatc cccatctcac aaatcgttgc ttctgttcct
    20341 ccagcaagaa tgacatcaga atagccatgt ttgatgttac ggaatgcttc cccaattgaa
    20401 ttcgttccgc tagcacaagc tgttacgata gctgtacaaa taccttttgc acctacacgc
    20461 aaagcaatat ttccagctgc catgttggca atcgccattg gtacgaacat tggagaaact
    20521 cgttttgcac ctttttcatg tagacgaata acttggtttt cgattgttgg aagaccaccg
    20581 atcccagatc cgatgatgac gccaaaccgg tcttgattca ttttttcttt atctaaaccg
    20641 ctcatctcca ttgcttctaa agcagcgtag attccataga tcgaaaacat atccattcgt
    20701 ttgctatctt ttttaataaa atatttatcg taagggaaat ctttgacttc acccgctaag
    20761 gtaatccctg tttctgaagc atcaaattta gtgatcggtg cgatgccatt cgtcccctct
    20821 tttaagctat tcaaaaagct gtctgcgtca tttccaatag gtgaagtgac tccatagcca
    20881 gtaattacca ctcgattcat ttgtttgctc tccttttctt atccgtgcat gaccaatccg
    20941 ccatcgacat tgatgacttg tccggtgata tatggatttt gtgctaagaa aactgctgtt
    21001 tgtgcaatat cttctacttg tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc
    21061 ttgactttgt ctgctaaaac ttccgtcata tcagttgtga tgaatcctgg cgcaatggca
    21121 ttacatgtaa tgcctctagt tgcagcttct cgtgccacag attttgttaa tcctactacg
    21181 ccagctttac tagctgcata atttgcttga ccaatatttc caatcaaccc agatacacta
    21241 gagagattaa tgatggcccc ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc
    21301 atattgaagg ttccaatcaa attgatatcc agacattttt tgaaatcttc tgcatccatt
    21361 cgcatcacaa gtttgtcatt tgtgattccg gcattgttta ctaacacatg gatcgatcca
    21421 agcttttctt ctgcttcttt gatcatttgt cccgctttct cataatcaga gatgtcaccg
    21481 gaaactccta cgcattttac accgaatgct tcgatttctt ctatcttttc ttttggaatc
    21541 tctccgcgac cattcaaaat gatatttgct ccagcttttg caaaagctaa ggccatcgct
    21601 ttgccgattc ctcttgtact acctgtgata aatacatttt ttccttttac atccataacg
    21661 ttcctcctta tagtattgct attgtttcat caaatgtctg cttattctca acacgcagaa
    21721 ctggtattgt tttatcgatt tttttcataa atccgcttaa tacttttccg gggccgactt
    21781 cgatgacttg ttcgatgccg attgttttta acttatggat actctcatag aaacgaacag
    21841 gttgcatgac ttgctgttca agtagtcccg cgattgtttc tttcttcatt atttccgttg
    21901 ttgtgttgct tatgatagga aaagaaggtt cagagaattg aatctgactt aggtccttcg
    21961 ccagtttttt tgctgccggt tctaaaatag ctgtatgaaa aggcccgctg acatttagag
    22021 ggatcattct tttgaaccct ttttctttta atagtaaaac agcttcgtct actgcttttt
    22081 cttcgccacc aatcacgatc tgctgaggtg tattataatt tgcaggagat acgatgccgt
    22141 acttgctagc ttcatgacag ctttcctcga tcgtttcaat cggcgcattc atcactgcaa
    22201 ccattttccc gcttccagct ggggcagctt ctgtcatata agcgcctctt tttgctacta
    22261 acgcaactgc ttcagtgaag gatagcgcat tactagctac taatgccgaa tattctccta
    22321 aactcagtcc taaagctgca tctgggatga ttccatgctc cttcaataag cgataatacg
    22381 cgatgctgac tgttagaata gctggctgtg tatattgcgt ctgatccaaa cgttcatttt
    22441 ctgtaaagca aagttcagcc atatcgtagc cgagaatctc acttgcttca tcaaatgtct
    22501 gctttacaac ggcttcttca tataaatctt ttcccatacc ttgatattgt gcaccttggc
    22561 cactaaataa gaatgctgtt ttcatgtttt tctccaatat atccagaata tatgtttgat
    22621 tcttcctatt caaaaaggcc gagacattcc tatttcagga aatgtcccag cccccaacct
    22681 aattttaatg attatgcagt ttgtttttct acgtaagtca ctaaatcttg aaccgttttg
    22741 attccgtctt ctgtttcgat tttcacgtca aattcatctt caatttcatt gatgatttgg
    22801 aataaatcca agctgtcagc ttctaagtct tcttggatgt cagtagtcaa tttgatttct
    22861 tcagtttctt ttcctaattc ttctgcgatg atttcttgaa ttttttcgaa taccataata
    22921 atatattcct ccgatattgt ttgcttttta tttttataat ttaacagtct acaactccat
    22981 gagaatactt ccccatgtta aacctccgcc atagcctgta aatattactt tctgctgttt
    23041 tcctaaggtg atctttccac tagaaactgc ttcatccaat aagataggga tgcttgcagc
    23101 agatgtattc ccatatttat ccatattggt caggaacttt tcttgcggca tctttacttt
    23161 tcgggcaatt ttctcaataa ttctgaggtt tgcctgatgc aaaagcagat aatcaactgg
    23221 tttgtctttc atgacttctt ggatattgtt tgcaacgtct cgtacggcaa aatcaaaaat
    23281 atctctgcca gtcatttgca aataatacga accttctgaa tcttctgaat agaacggatt
    23341 tttattttgc tggtagccag cggtcaatga gctacttctg gtaccgtcag ctgctaattt
    23401 ttctttcagc aattttttct gtggattcgc ttctaagaga atcccacctg ctccatcgcc
    23461 aaacaacaca gcagtcgtac gatcagacca atcgattagc ttagatagtg tttcaccacc
    23521 aatcaccagt cccttagttg agccgcagcg gatcattttt tcagccatgc ttaacgcata
    23581 aacaaatcct gagcacgcag cactgatatc aaaagcgata gcatgagttg ctcccaattt
    23641 cccttgtact tgtgctgcta cagatggtgt cgtgtaatcg ggcgacattg ttgctactaa
    23701 aataaaatcc aaatctttag ggtttagatt tttctgtttt atcaattttt ccgccactaa
    23761 aatgcataaa tcagaagtct cctgatttgt tacacatctt cgttcacgaa tacccgtcct
    23821 tgacgaaatc cattcatcag aggtatccat catcttggaa agatcattat tagtaataat
    23881 cttttcagga aggacgctgg ctgttgcagt aatcattcca aattgttcca ttaaatatcc
    23941 tcacttatag tcttgcaaga agtcgtgcaa gtttttcagt gcatccagca aggcatgctg
    24001 ttcctccgta gccattccgt ctaagatgtt cttgaccatt tcacgatgaa aatgctgatg
    24061 gacccgaaat agtaactttc cttttttcgt tagtcccaac tttacaacgc ggcggtcatc
    24121 ttcactgcgg atacgttcga catagccttt ttttactaac ttgttaatag cagtggtcaa
    24181 ggtcccaaca gtaattgata gttctttggc tacttctgaa gttgtttttt tcttgtacat
    24241 cccgattgcc tcgatcgtgt gcatctctgt gattgacaga tctttgaatt gagatttttt
    24301 cagctcagac tcttcgattg tcaaaatatc attgaagaca ctaacaagat aatcatttac
    24361 tgtttttaaa tctggttcca tgttccaccg tccactaatt ag
//
LOCUS       DAYYAG010000031        24143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000031 DAYYAG010000000
VERSION     DAYYAG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.031"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHARQ_001097"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHARQ_001097"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8409532.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHARQ_001098"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHARQ_001098"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8409533.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHARQ_001099"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHARQ_001099"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8409534.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHARQ_001100"
     CDS             complement(3373..4680)
                     /locus_tag="ACHARQ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8409535.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHARQ_001101"
     CDS             complement(4683..5264)
                     /locus_tag="ACHARQ_001101"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8409536.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHARQ_001102"
     CDS             complement(5836..7128)
                     /locus_tag="ACHARQ_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8409537.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHARQ_001103"
     CDS             complement(7125..7748)
                     /locus_tag="ACHARQ_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409538.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHARQ_001104"
     CDS             complement(7751..8587)
                     /locus_tag="ACHARQ_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409539.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHARQ_001105"
     CDS             complement(8604..9491)
                     /locus_tag="ACHARQ_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409540.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHARQ_001106"
     CDS             complement(9507..10790)
                     /locus_tag="ACHARQ_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8409541.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHARQ_001107"
     CDS             complement(11473..12156)
                     /locus_tag="ACHARQ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8409542.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHARQ_001108"
     CDS             complement(12174..12791)
                     /locus_tag="ACHARQ_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8409543.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHARQ_001109"
     CDS             complement(12801..13625)
                     /locus_tag="ACHARQ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8409544.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHARQ_001110"
     CDS             complement(13618..14178)
                     /locus_tag="ACHARQ_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409545.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHARQ_001111"
     CDS             14352..15308
                     /locus_tag="ACHARQ_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8409546.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHARQ_001112"
     CDS             complement(15613..16569)
                     /locus_tag="ACHARQ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8409547.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHARQ_001113"
     CDS             16673..17275
                     /locus_tag="ACHARQ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8409548.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHARQ_001114"
     CDS             complement(17363..18862)
                     /locus_tag="ACHARQ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8409549.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHARQ_001115"
     CDS             complement(18878..19402)
                     /locus_tag="ACHARQ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8409550.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHARQ_001116"
     CDS             19700..20449
                     /locus_tag="ACHARQ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8409551.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHARQ_001117"
     CDS             20440..21225
                     /locus_tag="ACHARQ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8409552.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHARQ_001118"
     CDS             21194..21910
                     /locus_tag="ACHARQ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8409553.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHARQ_001119"
     CDS             complement(22012..23031)
                     /locus_tag="ACHARQ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8409554.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHARQ_001120"
     CDS             complement(23050..24051)
                     /locus_tag="ACHARQ_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8409555.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7144 a         5211 c         4213 g         7575 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aat
//
LOCUS       DAYYAG010000032        23936 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000032 DAYYAG010000000
VERSION     DAYYAG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.032"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..1180
                     /gene="mvk"
                     /locus_tag="ACHARQ_001121"
     CDS             236..1180
                     /gene="mvk"
                     /locus_tag="ACHARQ_001121"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8409556.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1173..2150
                     /gene="mvaD"
                     /locus_tag="ACHARQ_001122"
     CDS             1173..2150
                     /gene="mvaD"
                     /locus_tag="ACHARQ_001122"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8409557.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2163..3248
                     /locus_tag="ACHARQ_001123"
     CDS             2163..3248
                     /locus_tag="ACHARQ_001123"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8409558.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3245..4309
                     /gene="fni"
                     /locus_tag="ACHARQ_001124"
     CDS             3245..4309
                     /gene="fni"
                     /locus_tag="ACHARQ_001124"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8409559.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4558..5361)
                     /locus_tag="ACHARQ_001125"
     CDS             complement(4558..5361)
                     /locus_tag="ACHARQ_001125"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8409560.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5381..6223)
                     /gene="kduI"
                     /locus_tag="ACHARQ_001126"
     CDS             complement(5381..6223)
                     /gene="kduI"
                     /locus_tag="ACHARQ_001126"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8409561.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6239..7246)
                     /locus_tag="ACHARQ_001127"
     CDS             complement(6239..7246)
                     /locus_tag="ACHARQ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8409562.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7253..7897)
                     /locus_tag="ACHARQ_001128"
     CDS             complement(7253..7897)
                     /locus_tag="ACHARQ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8409563.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7997..8755)
                     /locus_tag="ACHARQ_001129"
     CDS             complement(7997..8755)
                     /locus_tag="ACHARQ_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8409564.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8882..10033)
                     /gene="fucO"
                     /locus_tag="ACHARQ_001130"
     CDS             complement(8882..10033)
                     /gene="fucO"
                     /locus_tag="ACHARQ_001130"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8409565.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(10115..10426)
                     /gene="rhaM"
                     /locus_tag="ACHARQ_001131"
     CDS             complement(10115..10426)
                     /gene="rhaM"
                     /locus_tag="ACHARQ_001131"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8409566.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(10428..11273)
                     /gene="rhaD"
                     /locus_tag="ACHARQ_001132"
     CDS             complement(10428..11273)
                     /gene="rhaD"
                     /locus_tag="ACHARQ_001132"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8409567.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(11287..12543)
                     /gene="rhaA"
                     /locus_tag="ACHARQ_001133"
     CDS             complement(11287..12543)
                     /gene="rhaA"
                     /locus_tag="ACHARQ_001133"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8409568.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(12540..14000)
                     /gene="rhaB"
                     /locus_tag="ACHARQ_001134"
     CDS             complement(12540..14000)
                     /gene="rhaB"
                     /locus_tag="ACHARQ_001134"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8409569.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            14153..15022
                     /locus_tag="ACHARQ_001135"
     CDS             14153..15022
                     /locus_tag="ACHARQ_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8409570.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(15148..16047)
                     /locus_tag="ACHARQ_001136"
     CDS             complement(15148..16047)
                     /locus_tag="ACHARQ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409571.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16212..17036)
                     /locus_tag="ACHARQ_001137"
     CDS             complement(16212..17036)
                     /locus_tag="ACHARQ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8409572.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(17047..18342)
                     /locus_tag="ACHARQ_001138"
     CDS             complement(17047..18342)
                     /locus_tag="ACHARQ_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8409573.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(18358..18837)
                     /locus_tag="ACHARQ_001139"
     CDS             complement(18358..18837)
                     /locus_tag="ACHARQ_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8409574.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(18843..19871)
                     /locus_tag="ACHARQ_001140"
     CDS             complement(18843..19871)
                     /locus_tag="ACHARQ_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8409575.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(19888..20994)
                     /locus_tag="ACHARQ_001141"
     CDS             complement(19888..20994)
                     /locus_tag="ACHARQ_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8409576.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(21223..23820)
                     /gene="adhE"
                     /locus_tag="ACHARQ_001142"
                     /gene_synonym="adhC"
     CDS             complement(21223..23820)
                     /gene="adhE"
                     /locus_tag="ACHARQ_001142"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8409577.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         6756 a         5296 c         4337 g         7547 t
ORIGIN      
        1 aattgtattt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg
       61 ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag taaaataaag
      121 gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt
      181 ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt agattatggc
      241 aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc acgcggttgt
      301 ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg cattccttga
      361 agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa atttagccga
      421 agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa aacagcatga
      481 catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac gtggaatggg
      541 atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact tagcatttcc
      601 tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg cccatggtaa
      661 tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt ttacaaaagg
      721 gcatcctttc gactactttt ctttgaacat cgatgctttt ttgattgtcg ctgatacagg
      781 aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg aaacccagcc
      841 tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag caaaacaagc
      901 aatcattgaa aattcaccag aaacgttggc acagacaatg gatgaatcac aatcacttct
      961 ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag caaaagatac
     1021 cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga ttgcattagc
     1081 acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg gtgctgctga
     1141 aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca cgcgcatata
     1201 ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc cttccaatga
     1261 acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc atcttttctg
     1321 atagctatat ggtagatgaa ttttatctag atggcacctt gcaagacgaa aaagcaacaa
     1381 aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca ctaaaagctt
     1441 cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct gccagcgggc
     1501 tagctgcttt agcaggcgct tgcaatactg ctcttaagct tggattagat gatctctctc
     1561 tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc ggtggtttcg
     1621 tcgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc ccttccgatt
     1681 ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt
     1741 ccagcagaaa tgggatgcgt cggacagtcg aaacatccag tttttatcaa ggctggttag
     1801 attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa gatttccaac
     1861 ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact ttagctgccc
     1921 agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg gtgcgacaat
     1981 tgagaaagca aggcattcca tgctacttta cgatggatgc tggccctaat gtcaaagttt
     2041 tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta tttagcaaag
     2101 aacaagtaat tactgcacat gctggtccag gaattgcgat tattgaataa agggagattt
     2161 ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat gcagttgttg
     2221 aaacaggcca tccagcagtt atcgctgcag tcgatcaatt cgtgacagtc actgtagaat
     2281 ctgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct gtacgttgga
     2341 caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat tatatccttg
     2401 ctgctattcg cttgactgaa aagtatgcac aagaaaaaaa cattctttta tccttttatg
     2461 atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga ttgggttcta
     2521 gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg ttgaatttat
     2581 ctcagttgga gattttcaag attgcagcac tagccaattt agcagttcaa gataatggtt
     2641 cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca accttcgacc
     2701 atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt gccctggatt
     2761 ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta ttgattggtt
     2821 ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt tcgagagaag
     2881 ataaaatggt ggcttatcag cttttcttaa aaaatagtac agaatgtgtc aacgaaatga
     2941 tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga aaaaaccgac
     3001 aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca
     3061 aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt gcaggtgggg
     3121 gcgattgcgg aatcgtaatt gttgaccaga aatctggcat tcttccttta atgagtgcat
     3181 gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa
     3241 accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg ataaacaaaa
     3301 aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag cagtttctga
     3361 tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt atatcaatgc
     3421 gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag
     3481 agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag tcccttcttt
     3541 agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca ttgccaacat
     3601 cggtgcaggg acttctgtcg aaagagccaa agaagctatc cgattgtttc acgcagatgc
     3661 cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag accgtgattt
     3721 taccaattgg aaagtactta ttcaagaaac tcaaacagcc attgacgtcc ccctcatcgt
     3781 aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg ctttaggagt
     3841 tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg
     3901 tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag tctcttcatt
     3961 gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag gtatccgcaa
     4021 tgcctatgac atatttaaag ccctttgtct cggcgcaaat gccgtgggta cttcaggaac
     4081 cgtactgacg catctgatga atcacggcgt agaagaaact attatactga tgaagcaatg
     4141 gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg cggccctaca
     4201 ccaacaatct ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa gagggatcga
     4261 cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa gaacactttt
     4321 gtctcacgct ctaaacacgt acaaacggcg gaagtaaaag caactccttc ggaaataagc
     4381 tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt cttggagtta
     4441 aatgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct gtccttcggt
     4501 attcgttcaa tccgctgatt tcacaacctc tccactaaac agtgtactaa agctctttta
     4561 aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat ggccgttaac
     4621 gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga ttggctcgcc
     4681 ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac gttcaccgtt
     4741 ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg gtccaattcc
     4801 attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca gcatttttaa
     4861 tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac tcatcatgga
     4921 gcagatattg atgattttac cgccgccttt ttcgatcata tccggaataa ctgcttttga
     4981 aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg ccgcatccat
     5041 ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat caactgaacc
     5101 tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca
     5161 gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt catcaactga
     5221 ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag agcgtgcaat
     5281 ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat ctaaacgaaa
     5341 catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat tgatccattt
     5401 gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag atgaatgagt
     5461 aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg gcttgttcat
     5521 tgtctacaac taaatgtttt gtttcatctg gtttgcccat catgtggaac acacgtgtat
     5581 cttcattggc ataatcaaag tagacataag cttccatacg acgttcatgt gtatgacaag
     5641 gcatcgtatt ccaagcgctt cctggttcta aaatcgtata acccatttgt aattgacagc
     5701 tttcgcatac atttggatgg atgtattggt agattttacg ttcattcagt gtcaggcctt
     5761 caccagtctc cattggtttt acttggtcga tactgatttt tacatttgga tatttatgat
     5821 gagctggaac agaactaata tagaatttag ctgggttttc agcatcttga gaagaaaata
     5881 caacatgtcg tgtttctttt ccgatatagt atccgtcttg ttttttcatt tcttcttttt
     5941 gcccatcgat ctcgatgaat cctggtccac cgatattgat cacacctagt tcacgacgct
     6001 ctaagaagta atcaacacct agttctttgt ctaatgtgat ttctaattct ttttcagtag
     6061 gtgtcactcc accaaagatc attcgatcgt tgtgcgtata agtcaaactg attttccctg
     6121 gttcaaatac cttttctact aaaaattctt ttctcaattg ttccgttgaa taatgacgga
     6181 tatcttctgg actgtgtgta taacgtgttt ccatttcttg cattttcttc tactccttct
     6241 atcgaatgat ttttccagag ccgcatgcta aaaattgtgc gacttcttct tgactaaatt
     6301 ggttgcagtc cccatggatc gtatgcttca aggcagaagc tgccgtagca aaatcaatga
     6361 tttcttgcgg agacatattg gataaaatcc catggagcac gccacctgca aaagcatctc
     6421 cgccacctac tcgatccacg attggggtga ttctatggac ttgggattcc acatattcat
     6481 ggttcatcca caatgtcccc ttcaattcat tttcacttgc tgaatgaacg gtacgcttgg
     6541 tcgaataaaa cacctgaata ttcgggaact tttcctgcat tttttgataa taataaatta
     6601 gaggttgttc ttctgcttct ggcgctttct cgatacctaa caaataaaga gcatccattt
     6661 ttccagctga gcagatatct actaaaggta aaagctgatg gattacttcg ctggcttctt
     6721 gctgtgtcca taattttcca cgatagttga tatcgaagct gatcaggcag ccagcttttt
     6781 ttgcagcttc tatcacctgt ttcgtcattt tttgccattt tttagacaaa gctggggtga
     6841 ttcctgaaac atgaaatata tctattcctt ggaataactc ctctagcgac cattccaaat
     6901 gatccatttc agcaaaactt gatccggcac gatcatagat cactgaggcg gcgcgttcgc
     6961 cactaccagt ttccatataa taagtaccta gtcttgatcc accagataat acctgtttcg
     7021 tatctactcc ataacattgc aagtgctttt tgactgcttt tcccaatgcg ttatcaggta
     7081 cttttgtagc aaaaatcgct tcatgtccgt aattagctaa agaaattgct acgtttgctt
     7141 cccctccgcc ataatgggca gcgaatcgtt cacttgaatc gatacgggtt ccctgctctg
     7201 tggacaatcg caacatgatt tctccgagtg tcactacttt tcccataagc agctacctct
     7261 tgatttcttt catttttgct gcatactgtt gcgcgacttc cgttactttg tcaaaatctc
     7321 ctgtagctgc gggtgctaaa aggttcccac ctactccgac tgttacgaca ccagcatcga
     7381 accactcttt catattgtcc agactcacac cgccagttgg catgatattg acttgaggca
     7441 tcggcgcctt gaaggcagaa atgatacttg gtccataagc gcttccagga aatagtttga
     7501 cgatatctac cccgcttttc agcgctgttt tgatctcggt aatagtcata catcctggta
     7561 agtaaggaat ttgatacaga ttgcacatct ctgccgtttc ttgatcaaag gaagggctaa
     7621 caatatactc tgcgccagct aagatagcca agcgagctgt gacagcgtct aatactgtgc
     7681 ctgcgccaat cactatttct ggttggtctt tgtagaatga caaaagttct ttgattactt
     7741 gatctgcttg agggactgta aaagtcaact caataccagt tagaccacct ttgatgattg
     7801 cgtggcaagc attcaaagct tcttctttgc tttttccgcg taccaccgca atcactccgg
     7861 cgtttttcaa tcgagataaa atatcaattt ttttcatctt ctccacctat ttcctattta
     7921 gaaattttat tttataactt ggaaatctat acccatatta tacggcagaa aagaaaaatt
     7981 gcaaacgctt ttttcattat ttttgaattc tttgctcgat ttcgcttttt gtttgcttga
     8041 gggcactgat tatttgttct tttgtctcat ctgttaaacg atatttcggc atacttacac
     8101 taaatgcacc aatgatttgt tcgtttttca cgattgaggc tcctatacaa aaaatatcac
     8161 gttcgatttc ttcatcgtca aatgcgatgt gggtttcttt taccgttttg atttcctttt
     8221 tcaaccgtaa tgcattggtg atcgtatatt ctgtgtaagg gatcaacgga tgcttttcaa
     8281 gataatcagt gtactgctct tcactgaatt ctgctaatac ggctttgccc atcgcagaat
     8341 tgtataatgg tcgggtgatt ccgatttttg aagacatgcg gatcgtttga tgttttggat
     8401 ctagtttgtt cacgtacaag atctcattgt tgtccaatac ccctaagtgg atagtctcat
     8461 cgatcttttg ctgcagctgt tctaaaaacg gtaaagtcaa ctctaccaaa tcgatttgtt
     8521 caatgttttt attcgcgtag cgaataagct ttgcacctaa acgatagttt ttctctgaat
     8581 ttttatttac gtagccgatc atcaacaaag tatctagtat tttcaagact gtagaagagg
     8641 tcattcccac accctttgcg atcgtctgca aagagacatc ttggttttcc gctaaatagt
     8701 ctagtatttt agaggctttg atcaatacag ttccataagg ctttttattt tccatataat
     8761 attctcctat ttctttataa gaaaaattat agcggaaata ccatcaattt aaaagagata
     8821 tccagatata aactaccatt tctctttaca ggcaatttaa tgttgaagat ccgcttcgtt
     8881 tttacatcaa actttgatat aaagcaatga tttcttctac tgtcgtttct cttggatttc
     8941 ctggtgtgca tacatctcgc aaagcatctt ctgcaattgc tggaatatct gcttctttta
     9001 cacctaattc agaaatggtt tctgggattc caaccgcttt gctcaaccga tcgatctcac
     9061 cacaagccgc ttctcttaca tcttctaaag acatttcagc tgctccttta atccctagaa
     9121 caagagcgat ctcacggtac ttttctccag tatattcttt gttatatttc atgacagttg
     9181 gcaaaagtgc tgcacacgct acgccatgag gaatgttgta ccatgcactt aacgggtgag
     9241 ccatcccgtg taccagtcct aaaccgacat ttgagaagcc cattcccgca atatactgtc
     9301 ctaatgccat tttttctcgc ccattttgat cgccatctac agaagcttcc aaagaatggc
     9361 cgatgatttc gattgctttt aaatgcagca tatcagtcat ttcccaagcg ccttttgtga
     9421 taaacccttc aatagcatgc gtcatagcat ccatacctgt tgatgcagcc aattttttcg
     9481 gcattcccat catcatatcg ctgtcgatga acgcgacgat tgggatatca tgaggatcaa
     9541 cacaaacaaa ttttcggtga tttacttcat ctgtgatgac gtagttgatc gttacttctg
     9601 ctgctgttcc agaagtcgtt ggtatagcta atataggtaa acatggattt ttcgtatcag
     9661 caacaccttc taaactaatg acgtctgaaa actctggatt cgtcaaaata atcccaatgg
     9721 cttttgccgt atcgattggc gaacctccac cgattgcgat caaacagtct gcctctgcct
     9781 ctttaacaaa agccactcca tcttttacat tttggatcgt tggattaggt acgatcccgt
     9841 cgtataaagc ataagcgatt gctgctttat ctagaagttc tgttactttt gttgctatac
     9901 cgtgctcgat caatcctttg tcagtaatga cagctgcttt gccgaatcct cgctgctgaa
     9961 attcagggac gatcgactca atcgctcctt ttccaaaata cgatgtttca tttaaaatca
    10021 ttcgattact cattttctat tcctcctaat ttgtattatt tttcacaata aaaacgaaag
    10081 aaccccaagc aaccggttgc ccagttgctt gttgctacag ttcaaagact ggcatcaatt
    10141 cccaactgac tggagaggca tctggatttg tctccatgat atcttccatg tacttccacc
    10201 atttttgatt gatagatgta tttgccattt tttcccataa cttttcatct tcgatcacta
    10261 agtaaccaaa taacaaactt gtactcggat caagatagat cgaatagtcg atgaccccgt
    10321 gttcacgaag tgctgtacgc atttctggcc aaagttcatc atgtcttttt ttgtattctt
    10381 cttgtttttc tgggtaaact ttcatgcaaa aagctttttt caccatgtca gacacctgct
    10441 ttcgtatgaa cttcctccaa ataacctgct tttggagtga caccaaacgc atctgctaaa
    10501 tgccacaggt tttcgtccgt gattgtttgc aagattggtc cttgcgcttt gacgtacgta
    10561 tagacttctg ctgccttttc tgctgtctcg atcaaaccaa agacttcatc catgtctttt
    10621 cctgacccgt agattccatg ttgtggccat aaaaccaatc gagtttcttt cattttttca
    10681 gctgttgctt ccccaatctc atttgttccc ggaaccatcc acggaatgat ggaaattcct
    10741 tctggaaaaa caactaagca ttccgtacac atttgccata gcgtacgact aaaggaacgt
    10801 tcgtctaatt catgcgtaaa actcatcgct aatagatgac tagcatggca atgcataacg
    10861 atccgattat ctgggtctac ttctaaacgg gcgatatgac tcatgaaatg acttggcaac
    10921 tcgcttgtag gaaggcattc gttttccagc ccccacaaaa gttccagtgt tttgccatct
    10981 tcagcaactc gaacgattcc aagattctcg gcaggttcgc tcgctacatt tttaaaatat
    11041 cttcctgaac cagttacaat aaaatagcat cctgctaaac ggctggcatc aaaaatcatc
    11101 gagatgcggc ggatcacttt ttcaggatcc aagaacggtg tgatctcttc ttcttttagt
    11161 aaataagaaa tattcccgcc atttctttca tcccatccca agcgataaag attcgacgtc
    11221 gtttcgatca tttctttcac ataaggcgct tgtaaaatat ctattttttt caatatagcc
    11281 acactcctat tttcttaatt ccaacacgtc tttttgatat tcctggactt cttccagcca
    11341 atccaaacca actggaactt gatttttgta acagaaataa ttccatacat cggcatacgg
    11401 gaaatctttc aattcttccg tataagccat tcttgaagta aagtcaaaag ccagctccat
    11461 ttctttcaat cgctcaactg gttccagcat agctttcagc agtgcttttt gtgtatttct
    11521 cgtaccgatg acccaagcag cgatacggtt gatcgttgca tcaaagaaat ccagaccgat
    11581 atttgttttt tccaacaatt cgttacggac taattcttta gcgatttcct gcaattcatc
    11641 atccatcaaa accacatggt ctgaatccca acgcactgga cgggaaacat gcaacatgat
    11701 tcctttgctg aataaagcta atgaagataa tttattggaa atcacttctg ttggatgaaa
    11761 atgtccagca tctagacaaa caagtttgtc acgagtcaac ccgtaaccca tataaaattc
    11821 atgggaaccg actgtataag cttcagcgcc taaaccaaat agtttgcttt ctactgcatc
    11881 ttgtacatac cgttcatcta actcttctgt aaaaatttca tcgagtgatt ccattaaacg
    11941 ttcccgtggg gtaaaacgat caatcggatt atctttcatc ccatcaggta cccaaaagtt
    12001 attgatactt gtttgcccag tttctttccc aaagtactcc gcgattttgc ggcttctttt
    12061 cccgtgttcg atccagtatt cacgaacttc tttatctgga tgagccaatg taaagccgtc
    12121 tttcatcatt ggatgcgaaa agaaagtcgg attgaagtca agaccgattt cttctttttt
    12181 tgcccactct acccattttt caaaatgttt cggctcaatt tcatttagat cgacagattc
    12241 ttctgtatcc aaatagatag catgaaggtt caatttgtgt ttgcccggta tcaacgaata
    12301 agccttctcc agatcttgtc taagctcttc aggtgttcgt gcggctcctg gaaaattccc
    12361 tgtcgccatg attccaccgg tcaattcgcc atccggtgtc aagaaacctt tgacatcatc
    12421 tccttgccag caatgcatag aaattttgat gtcctgtatc ttttctagta cagcatctgt
    12481 atctacaccg atagttgcat atttttcttt tgcttgttcg taatttcttt caatcgtgtt
    12541 catacgatta ctcctttgat tttattcacg taccattcca tcgtgttttg cttattttca
    12601 tctgggtaaa aagtttcaca tggaaacgac tgtttgatta catttcttgc ttgttctata
    12661 ttcgaaaact gacctgtact gagcatttgc atggcaacat tcccgattgc tgtggcttca
    12721 ctaggtcctg cttctacaat gattccggca taatctgctg tcaattgatt cagtagacga
    12781 ttattcgatc cgccccctac gatgtgcagc ttttccaatt tcgttccagt taagtgttct
    12841 actttgatca gctcttgtgc gtaacaaaga gcaagattat catacacaca acgagcgata
    12901 gctcccgatg agacagggac tggctgctct gtttcaaaac agtattgctg aatctcttct
    12961 atcatatttg ggggattcaa aaatcgaggg tcattgattt ccatcacttg ctgcagcggt
    13021 ttttcttttg cagcaagctc tgccaattcc gcataactgt actcgtactc ttgcattcgg
    13081 gcaacttcct ggatcaacca catccccatg atgtttttca aaaaacgcac cgtgttatgg
    13141 gcaccccatt cattcgtata attttcagaa aaagcattgc gagaaacatt ggcgatcttt
    13201 gtttcaatcc ctaaaagcga ccatgtccca ctgctgatat aaccccaacg ttctccttga
    13261 cctggcgttc ctaatacagc tgacgccgta tcatgacttg ctactgtgat aaaggtcaca
    13321 tctggcaaat catattttga aaattgttct ttttgcagat ctcctagtat cgttcccggc
    13381 tctgtcaatg gtggaaataa ttccttcgag acaccgattt tttcaagaag ttctatttcc
    13441 cattcttttg tccctgcatt cagcagctgc atagtagatg cattagtttt ctccgtcacc
    13501 atgttaccag taaagacata ccctaaataa tcaggtatca gtaacaattt atgtgctttt
    13561 gccaaatttt ctttttcttc agcaaacaat tggaacaagg tattgaaagg ctggatctga
    13621 atccctgtct gctcatacaa cttatctaga gaatagattt cagaaaactg ttctactgct
    13681 ccattggtac gcttgtcccg ataagcaatc ggatcattta tccgatcccc ttgttcatca
    13741 atcagacagt aatcgactgc ccatgtatcg atccccgcaa aaaggcgatc aatccctgtc
    13801 tgctttaatt tttcaaatcc aatcaaaatc tctttgataa gataatcgat cttccagcga
    13861 tcgtgaccgt ctttgttccg aaagccgttt ttgaaccggt gtacttcttc caagatcaaa
    13921 gaaccttttg catccctcgt acttttcatt agtcgtccgc ttgatgcacc gatatcgatg
    13981 gcaacaaatg ttggattcat ttctccacct cttttacttt attgttgatt agcactttat
    14041 taataaagcg ttatcattta ttgacaatgc tatcataata cagaatctta tattttgctt
    14101 cacactattt tggtatataa ttgcattatc ttgacaagtt gggagtgttt acatggatta
    14161 ctgggaaaaa gccaaccact catggtcgga agactcaaaa agagtgatct tgacgcctac
    14221 acaaagaagc aggaatttat ttttttatat ccaagaaatc ggttacttca aagcaccaat
    14281 cccttactat actgaacgag cacatttacc ttcttacttg ataaagttta ctttgtctgg
    14341 caaaggttcg cttacatata atgataatca gcaacagcta aaagctggag acttattttt
    14401 tattgactgc caagaatatc agcgttatca aacgatcagc gaagaaccat gggaaatgga
    14461 ttggatccat ttgaatggtc agaacgtcca atcattttat caggagttca caaaagacgg
    14521 ccataacacc ttccattcaa caggagatcc atcagaaaac aagatccacc atttgatcac
    14581 agaattgatc cacatgcaac aaggcacgaa tgcccgtacc gatttccagt cctctgtagc
    14641 tatccacgag ttgctgaatg aactgatttt gcaaaagtat cagctagatt tcgaagaaga
    14701 ggatattccc gcctacgttt acaaactgaa gcaatactta gacgaacatt tcaaagaaac
    14761 gattacactt gatcaattag aacaacaatt cttattgaat aaatatcagc tgagcaaaga
    14821 tttttccaaa tttatcggtt ctccaccaat cgattatttg atcagcaaaa aactttctta
    14881 cgctaaagac cttcttcggt atacagaatt tacggttcga atgatttccg aagaaatagg
    14941 gattgagaat tttgcttact ttagccgcct gttcaaagca aaaacaggac tatcgccgag
    15001 agactttcgt aaattcagct gattcatata atatgtacgt cattttttca aaatattttg
    15061 aaatatatag ataaaaataa aataaccgca gtttctgctt ctatgtaaac aacagcagct
    15121 actgcggtta ttttttagtg atcattctca ttgttcatct tcaaaatgtt cataatgaac
    15181 caactgtttc tgtgtatcac gtttcaaaaa ttctttaggc gttatgccga actctctctt
    15241 gaatgctttc agaaaatgcg gataagaaga aaaaccactt ttactatata cagcactaga
    15301 gctgcggtat tctcgcaaca agtgcttggc atagatcagc tttttcttca ttacatactg
    15361 atgaaacgta aatccagtgt actttttaaa ttctcgtgtg atatggtatt tgctgacaaa
    15421 aaactgcttt tccatttcct ctaaagaaag cggatgagat aaattttttg agatatattg
    15481 taacatttgt tccacacgct cattaggaac tacagcattc tgttcttgcg cactctcttc
    15541 tttttgtaca acttgattga gaaaaatcaa gtattctacc agagcttgtt catattttat
    15601 gtcttcaccg taaatttttt tatcaggagg atggtcgata aatgataatt tttgcttcaa
    15661 tacttcctgt tctatttgca atacacgact cctcttctct cccattggtt gaaaacaagc
    15721 cgatagatta gaccatggcg tggaacgact ttctagaaaa gaaggggtga caaagatata
    15781 gacacgctcg aatacatctg aagattggcg gacgatacga tgcaaatcat tggaatggat
    15841 gagcagcaca cttcctgctt tgatcgtaaa ttgtcgtccg ttcaaataaa aagtcgcttc
    15901 tccctttaat gtagcatgga tctcatagaa atcatgatga tgatagatcg ttttattttg
    15961 gctgacgaca tctttgacat gatagatttc atactgatca gaaaccatcg tattgacttg
    16021 ttcaccgctg attttctttt ggagcatgtg ccgcacctcc ttttgttcac ttatgcacaa
    16081 ttttattata ccacgttttt tgtaatcaaa aagagctgct tttatatact cttataaaaa
    16141 agaagagacg tggcatcttc tagccccgcc ccctccattc taaatattag ttaaccaacg
    16201 tcttctttac attattcaag aagacttctt ccataacgaa tcccatcatc ttttgtttct
    16261 tcaagaacca catacaggta cggacgattc ttcatgatga tttctttgat ttttgtgtac
    16321 tcgatcacac cgtctcccaa atttaccggc acgatttttt cctgttcgac acgaaaatct
    16381 ttcaaatgaa ccgcacatat cttttcacca aaacgttcaa aagcttcttc taccaattgg
    16441 acttgttctt gataatcact acttgtgatc aaattggtcg gatctaaaat gattcctaga
    16501 tagtccgaat ctactgcttg gatcagctgc tctacccttg ctaaagaata caccggatga
    16561 ttcaaacctg gttctatccc caccatcatt tggtattttt ctccagcttt taccaagcga
    16621 cggataacgg acacagcttc agcaaatgct tcatctgtga aattttcttc cgtatattgg
    16681 atttccggca aaacacatcc agtttccgaa gcaaccatcg acgcaccaaa ggaagaagca
    16741 tagcgaagat actgctcgaa ctttgtcagc aactcttgtc gtatcgttaa atctggatga
    16801 atcatattga tatagcaact taaaattcct acggtcactt cttgcttttc taaaactcgt
    16861 ttgacataat ttcccatccc agaattgatc tcatttgcgc cagaaggtat ttcaggaaac
    16921 gaaagaccta aggcgagctg cagtgtatga atcccttgtt cttttgtttt ttctgcaaga
    16981 tcttctatcg tttggacttc ggaaagatca tgtcctctaa ttgtaatgtt caacataact
    17041 attctcctaa ctcagcatat tgggtagcca catgacgatt tgcgggaagt aagtaatcaa
    17101 aaacaagacg actaagataa ccccataaaa aggaagaagc ggtttcagca ttgcctctgc
    17161 ttttacgcct cctacacttg caccgacata tagtcctgta cctactggcg gcgtgatgga
    17221 gcctacacac agattcatga ttgaaaacaa cccgtaatgt acaggatcaa cccctacact
    17281 tgtgacgatt ggtaaaaata ttggtgtaaa aatcaagata gcaggcgcca catccataaa
    17341 catacccacc agtaataaca caagattgac taataacaga ataataaatt tattatccgt
    17401 tacgcccata atcaacgaac taattgcctg aggaataccg gtaaacgcca tggcgaaaga
    17461 catcatggaa gaagtagcca ataagaacat gatcgtccct gacattgcta ctgattgaaa
    17521 caacatatta ggtatatctt ttagttttac cgttttatag taaaacatag caatcaacag
    17581 ggagtaaatg acagctatcg ctgaagcttc tactgctgta aatatccccg tcaagatacc
    17641 accgataatg atgacaatca atagaaggct aggaatcgcc tcttttatta tttttccagc
    17701 atgagccatc tgtccttttt cgacaacagg gtatttattc tttttagcaa cgatgaatgc
    17761 tactagcatg attcctagcg cccacaaagc gcctacgaga taacctccca taaacaaagc
    17821 agagatcgaa gcaccaccag caacaagtga atacatgata aaagcagtac ttggaggaat
    17881 gaccatacct gtcggagctg acgcgatatt gaccgctgtc gcaaacttcc ggtcatatcc
    17941 ttcttccact tgctgaggga tcaacactcc gccaatcgcg gtagaagcgg caatggccga
    18001 gctagagatg gaaccaaata aagcattccc taacacattc gtatgagcca aagcccccgg
    18061 cacgcgcccg acgaacaaca tcgcaaaatc cactagtttc ttagctatcc ctccattatt
    18121 catgataatg cccgagagaa cgaaaaacgg aatagcgatc agcgaaaaac tattgatact
    18181 tcccaccatt ttttgagctg aactaaaagc agaaacatca aacggtataa ctaacaataa
    18241 agtcatcatc gatgagaatg caatacttat agcaatcggc atccctaata caagcaacac
    18301 aacaaataca aaaaataaca ctaatccggc ttctattgcc attttattcc tccattttta
    18361 tattttttct tgaattgctg gcttgttgct taatgtatcg attagattga tcaggctata
    18421 gataatgatg agtacccccg atacaggtac tgacagataa acatatccca taggaatact
    18481 taaagaaggc gagatctgcc ccattgtcaa tgtgacagca ttccatccgc catacaccat
    18541 gatcatcgca gcaaatacca gaaataaact ttgaacaaca acttctaaaa ttttactttt
    18601 tttgccagtt aatttttcac ttaaaaccgt tacggctaaa tgtgcttttt gtcccacaac
    18661 atacgcggaa gacaacatgg caaaccagac aagaccgaat cgaacaagct cttccgtgac
    18721 cgtatttgga tttcctaaaa ccgttctgga aaatacttgc cataaaacaa cagcgaccat
    18781 cccaacaagc aaaacagctc ccaggatctc taataccttg tttaaaatca ttttcatcac
    18841 agctacgctc cttgctcttg gatcttttga taaaggtctt tgtaggtagg atcgtttttg
    18901 aatatatcat gaagtggggc aactgcctct cgaaacggtg tattatctac atcattgaat
    18961 tggactccat gttcttcttg tgctaaagct ttttcttctt caattgcttt tgcaaaagct
    19021 tcaatttcaa aaaccgttga ttctttcgct gcttcttcaa tggctttttc ctgctcatca
    19081 gttagctgtt ctttgatggc atcattcatg atgatgatat ctggcacacg tgtatgctca
    19141 tcataggaat agtatttagc cacaccacca tgtccagctg tatacaagac aaattcattg
    19201 ttttctgatc cgttgatcaa attggattga agagacgtgt acacttcatc acttcccata
    19261 ggtacgggcg aaccgcctaa aagctctacc attttgatag cggtttcgct ttgcatcaca
    19321 cggatcttct ttcccttcaa atcttccggc gtatggatcg gaccgtctac catataaaag
    19381 ctgcgctgac cagaatcata ataggtcaat ccggtaaatc ctaaattttt ggtcgattgg
    19441 tagattggct ccattatttc ttcattatcc atcaccttaa agaaatgttc ttcatcattg
    19501 aatagataag gaactgaaaa aatcgaataa tctttagaga aactttccaa cgctgaggca
    19561 cttacttttg tgaaatctac tgctcctgac tgtgtcaatt cgatcagttc acgttctcca
    19621 cccagttgtc catctgggaa atactggatc tgtacggaac catctgtttt ttcttttact
    19681 aaacgcccaa attcattcat tgcatcaatt actggctggc tgttgcttgc ataagcaaag
    19741 cgcaaggtaa ctatctcatt tacatctgca tctgcgacag ttgccacact agaattattt
    19801 ccgcagccgc ttagacctag cagacctagg ctaagcgaac ttgctgcaaa cagcagtgat
    19861 gtttttttca tatgggtttc ctttctttta acaataagag acgagcaatt ctgtcaaaca
    19921 aagcaccgtt aacgactgtc cataaggcat tgctgtgatt ccgatattcc gataaaaatc
    19981 taaactgtcc cccattcctg tcccgattga aacgttttga acttctcctt tttcatcgat
    20041 ttgttcaagc aatccttgga tagcacgata agcgacttct ttatattctt gtggtaaata
    20101 acgtttatgg accgctttca aaataccgta agcaaatcct gcagttgcag aagattctac
    20161 gtaagaagaa ggatcatcca acaacgtatg ccataaaccg ctttcagact gataagtttt
    20221 taatgcacgc acttgtgctt caagtgtttc gatcaataat ttgcgcaatg catcttcttt
    20281 cgataattct aagatttcaa tgatctctgg aatagcaatc gtcaaccagc agtttccacg
    20341 agcccaaaat gcttcggcat agttatgatt tccttcaaac gtccagccgt gataccacaa
    20401 tcctgttcgt ttatccatca agtatttgat atggatcata aactggtaac gagcttcttc
    20461 taaatattct gggcgattca acagtttccc gatttttgcc aaaggaagca ctgtcatcat
    20521 caatgtatcg tcccacaact gattcttatt ttccggtcca tatgtggcat gctgtaaccc
    20581 gtcttcattt gtccgaggca tgtcattcat gacccattct gcccattgtt ctaaataagg
    20641 caaatagcgt gaatctttcg tgtcttcata caagtaagcc atagtcaata gcggtgccat
    20701 tgtattgacg tttttgggcg gagcgccttc ttccatacga tcctcaaacc attcattgac
    20761 cacttgataa gcgcgaggat tttttgtcat ctggtaattt ttataaatac catatagacc
    20821 gacaccttgt ggccaattcc acactgtcca gcttttgtca tctactttca gtccatcaaa
    20881 gtccaacaaa aattcaccat tatcgtcttt gatcgttgtc aggttttcaa cgagcaggtc
    20941 aattttttct ttcactaatt ctttaggtac aaaaatctct tctttaataa tcatctttac
    21001 gccacctttt taaacgcttt cattttatat ccttaatata gcagtaatac cgtccactat
    21061 aaatatcgct ttttgaacaa aaactgtcta aaattgacct aaatgttaaa taacaaaagt
    21121 aactttcagt tacttggaaa ttaaaaaaag aacttggaga aacaacgttt tccaagttct
    21181 ttttcatcta ttttcttatc ttttttctac gctttctttt gcttatccgt tgtaagcatc
    21241 aatgatgatc tgtttcaatt cagagatcaa tggttctttc ggattagctg tcgtacattg
    21301 gtcttcgtaa gctaattcag ccatacggtc tacggttgta tctagtgttt cttgtgtgac
    21361 tccttgagct tttagactca tgttgatgcc gacagatttt cctaattcat aaacagccgt
    21421 tgctaaagct tcgactaatt cctctgttgt ttcgcctttc agacctaaga attttgcgat
    21481 atctgcgtaa tctgtatctg cacggaaata gtcatattta gggaacatcg catgtttttg
    21541 tggatctttt gcattatagc ggataatatg cggtaaaaga atcgcatttg tccgtccatg
    21601 agggatcccg tattccccgc cgattttatg tgcgactgag tgacaaatac ctaggaatgc
    21661 gttagcaaac gccataccag ccattgtaga agcgttgtgc attttttcac gtgattccat
    21721 atcaggtgtt tttactgatt tttctaaata gtcaaagact aacttgattg cttgtaggct
    21781 caatccacgt gtataatctg aagccatgac tgaaacatag gattcgattg catgtgttaa
    21841 cacatccatc ccggtatctg ctgtgacaga tgctggaact gacatgacga attgaggatc
    21901 gacgattgcg acatctggcg tcaatgcata atctgctaac ggatatttca catgtgtttc
    21961 actgtcagtg atgaccgcaa atggcgttac ttcagaacca gtccctgatg ttgtcgggat
    22021 acatacgaat tgtgtttttt ctggtttgtc gatcttatat gtccgtttac ggatatccaa
    22081 gaatttttgt tttgcaccaa agaaagatgt gtctgggtgt tcatagaaca tccacattcc
    22141 cttagctgcg tccattgcag aaccgcctcc aagagcgatt actgtatctg gcttgaagtc
    22201 tgccatcagt ttcgttcctg cgtatacggt atttgtcgat gggttcggtt caacatctga
    22261 gaagacttcg atcttcacat catttttacg tcgtgccaat acttcacgaa ctgtatcgca
    22321 gtaaccgaat tggaccatac cagggtcaca aacgatcatg acacgttcaa cgttttccat
    22381 tttctctaag tacaatagag agttcttttc aaagaagatt tttggcggta atttgaacca
    22441 ttgcatgtta tttctccgtt tcgctacagt tttgacattg attaagttta cagcagagac
    22501 gttacgagaa acagagttct taccataaga accgcatccc aaagtcaatg acggaatcat
    22561 ttcgttatag atatcaccga tgccgccttc tgcagatggt gtattcacta aaatacgaca
    22621 agctttcatt cgaagaccga atttgacatg caactcttcg ttttctgaat ggataactgc
    22681 tgtatgtcct aatcctccaa gattcaacat accttcacac aaatcaaacg catggtccgt
    22741 gttgttagct ttgatcatgg caagaactgg agaaagtttt tctcttgata gcggataatc
    22801 tgggcctacg ccttccaatt cagcaatcag cattttcgtt ccttttggta cactgattcc
    22861 tgccaatttt gcgatttcca tcgcagaatg tccaacgatt gatggattga ccgcatattt
    22921 tccttcgttc atcactgcat cttctaattt ttgcaattca tctggtttta cgatataaac
    22981 ttggtgtgct tggaattctg cttttactgc tgcatagatt tctttatcca cgatcacagc
    23041 ttgttcagaa gcacaaatca ttccattatc aaatgttttc gaaacgatca aatcgttcac
    23101 tgcacgttta atttttgctg ttttttcaat ataagctggt acgttacctg gacctacacc
    23161 taaagctggt tttcctgtag agtaagcaga cttgaccatt ccagctccac ctgtcgctaa
    23221 gacgatcgcg atacctggat ggttcattag catagaagtc gcttcgatcg aaggatgttc
    23281 gatccattgg atacaatttt caggagctcc ggcagcgata gcagcatcac gtaccactcg
    23341 tgctgcttct gcagaagatt tttgggcact tggatggaaa gcaaaaacga ttggatttct
    23401 tgttttcaac gcgatcattg atttgaaaat cgttgtggaa gtcggattcg ttgttggagt
    23461 tactccgcaa accacaccga ctggttcagc aatttcaatc aacccttttt ggacatcttc
    23521 attgatcaca ccaactgttt tatcatgttt gatattatgc caaatatatt cggacgcata
    23581 catatttttg attgctttat cttcgtaaac accgcggcct gtttcctcta cagccatctt
    23641 cgctaatggc atatgttgat ccaaagctgc cattgccatt tcatgcacga tatggtctac
    23701 tttttcttga tcaaagcttt ccatttcttt caatgctgca tttgcttttt ctgccaaatc
    23761 atcaatcatt gcttgtacgt caattgtctt cgttttttct ttttctatta ctttagccat
    23821 cattatcctc ctaatgggtc atggttattt gttaaataat tcacatgtat atcatacaat
    23881 atttattttt ctatgtaaac acttttttgt gatttttttc acagttattt ttaaat
//
LOCUS       DAYYAG010000033        23058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000033 DAYYAG010000000
VERSION     DAYYAG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.033"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..225)
                     /locus_tag="ACHARQ_001143"
     CDS             complement(<1..225)
                     /locus_tag="ACHARQ_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409578.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKN"
     gene            complement(232..780)
                     /locus_tag="ACHARQ_001144"
     CDS             complement(232..780)
                     /locus_tag="ACHARQ_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8409579.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(825..1157)
                     /locus_tag="ACHARQ_001145"
     CDS             complement(825..1157)
                     /locus_tag="ACHARQ_001145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409580.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1171..1428)
                     /locus_tag="ACHARQ_001146"
     CDS             complement(1171..1428)
                     /locus_tag="ACHARQ_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409581.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1623..2165)
                     /locus_tag="ACHARQ_001147"
     CDS             complement(1623..2165)
                     /locus_tag="ACHARQ_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8409582.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(2186..3277)
                     /locus_tag="ACHARQ_001148"
     CDS             complement(2186..3277)
                     /locus_tag="ACHARQ_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8409583.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNFTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3285..5495)
                     /locus_tag="ACHARQ_001149"
     CDS             complement(3285..5495)
                     /locus_tag="ACHARQ_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8409584.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5511..8039)
                     /locus_tag="ACHARQ_001150"
     CDS             complement(5511..8039)
                     /locus_tag="ACHARQ_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8409585.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8091..8501)
                     /locus_tag="ACHARQ_001151"
     CDS             complement(8091..8501)
                     /locus_tag="ACHARQ_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8409586.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8514..8732)
                     /locus_tag="ACHARQ_001152"
     CDS             complement(8514..8732)
                     /locus_tag="ACHARQ_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8409587.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8729..9721)
                     /locus_tag="ACHARQ_001153"
     CDS             complement(8729..9721)
                     /locus_tag="ACHARQ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8409588.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9800..10711)
                     /locus_tag="ACHARQ_001154"
     CDS             complement(9800..10711)
                     /locus_tag="ACHARQ_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8409589.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10730..11074)
                     /locus_tag="ACHARQ_001155"
     CDS             complement(10730..11074)
                     /locus_tag="ACHARQ_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409590.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11094..11372)
                     /locus_tag="ACHARQ_001156"
     CDS             complement(11094..11372)
                     /locus_tag="ACHARQ_001156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409591.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11731..12231)
                     /locus_tag="ACHARQ_001157"
     CDS             complement(11731..12231)
                     /locus_tag="ACHARQ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409592.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12232..12777)
                     /locus_tag="ACHARQ_001158"
     CDS             complement(12232..12777)
                     /locus_tag="ACHARQ_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409593.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12774..14030)
                     /locus_tag="ACHARQ_001159"
     CDS             complement(12774..14030)
                     /locus_tag="ACHARQ_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8409594.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14390..14701)
                     /locus_tag="ACHARQ_001160"
     CDS             complement(14390..14701)
                     /locus_tag="ACHARQ_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409595.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14724..16199)
                     /locus_tag="ACHARQ_001161"
     CDS             complement(14724..16199)
                     /locus_tag="ACHARQ_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8409596.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16648..17100)
                     /locus_tag="ACHARQ_001162"
     CDS             complement(16648..17100)
                     /locus_tag="ACHARQ_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409597.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17102..17467)
                     /locus_tag="ACHARQ_001163"
     CDS             complement(17102..17467)
                     /locus_tag="ACHARQ_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409598.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17566..21849)
                     /locus_tag="ACHARQ_001164"
     CDS             complement(17566..21849)
                     /locus_tag="ACHARQ_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8409599.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21996..22235)
                     /locus_tag="ACHARQ_001165"
     CDS             complement(21996..22235)
                     /locus_tag="ACHARQ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409600.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22325..22567)
                     /locus_tag="ACHARQ_001166"
                     /pseudo
     CDS             complement(22325..22567)
                     /locus_tag="ACHARQ_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22680..22916)
                     /locus_tag="ACHARQ_001167"
     CDS             complement(22680..22916)
                     /locus_tag="ACHARQ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409601.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6777 a         4991 c         3393 g         7897 t
ORIGIN      
        1 atttttactt gttatttttt cattgactat aactagttca tctggtaaaa aaattaacca
       61 acaaacatcg acaaacagta aatgatataa tccatttcca cctccaagca tattcaatac
      121 tggtgaacat gccttagaaa cacgaatata gttatggttt aaatagatat tttctttaaa
      181 cattgcttgt ttaaaagcca tatgctcttg ttctaaactt ttcataaact tttactctaa
      241 tgcacagcta gcagcagctg ctgcaattcc tgctacaata tttaccttag ctaactcttt
      301 agctgcagct tttcctaatt gtttttctgc aatttttaat aataaatccg cagctttttc
      361 aaaattaaaa gcttttaatg cttcaataaa atctccctgc gtacctatag cccaagccac
      421 tcctcctcca ggaagagaat taatggctac acatccagca tatcgtttcc aatcaacttt
      481 ctccattcta ggaacaataa tatgttcata aaagactctt gctgctcttc cttgttcgtt
      541 atcttgactg attcgttcac gaatagcacc aaaatccttt atatggtata caccatgttc
      601 gtctaaaaaa ccaactgtat caaaataaaa agttaattca tctactaatt tattctttat
      661 ttctaaatca actgtattca ctttttctgt ggcatacaca cttgttccag tactaacaaa
      721 agtacttaaa cctaataaga aaacacctaa aacaccaaat attttaatta ttcttttcat
      781 aatttctcct ccataaaaaa taagttactt tttaaatatc ttgtttatga attcattccc
      841 caaaggcctt ttaagattga tacagcagtc cgttcacttc ttttagactt gtcatacaag
      901 tcttttaaat gatcccatat cagactagtt tgtctatcta gcttcaatcc attaaacgct
      961 aatgaacggt tcattttttc tattacttta gataaagcta cagaaatttt tataccttca
     1021 caacgcgcat cttggatttc aataatagta tcatcaatta gtttttgaat ttcttgccta
     1081 tcaacatatt caatcttaga aagagaatct tttaaagcta ctaatgattc aacgatttct
     1141 tttttctctt cagacatttt cttcctcctt ctagtgaccc gtaaagccat gtagagcaga
     1201 gtccattgaa ccagtaatta cactatctac aacgccattc caatttactc tacattttcc
     1261 atttttcaga tttgtcggtc catacggata gcctttttga cattcaatgt tcggattata
     1321 ccagatagga ttataccatt cagccgcaga agcagattca gtcgatacaa acaatcccac
     1381 accaacaaat ccaatacaca tcacactata gccaattagt tttttcatct cgtatccatc
     1441 tcctaaaaaa tagaatagtt cttatagtta ttcataagac atattttttc cgtttttgtt
     1501 ttatcctttc tgtcctataa caactttttt caagtaaaga atgacatagt ttttcttaaa
     1561 aaaacagtct gtttttttct atttcttcgg tgtataataa atatgagtgt tcatttttta
     1621 ttttaatacg cactccgtac ataataaatc ttgatcgcat caatcttttg agcttcttga
     1681 cctactttca attgaagtaa tacaaattga gtaaccttat tcattgtttc ttctccaaat
     1741 aacaaatatt gatccggatt ctctatatct tgtgtcacct ggtaaagttt cccagttgtt
     1801 ttcatttgta catgcgggtc tacatcaata ctattgtctt taatacaacg atctgtcata
     1861 tagtccctca cagcttgatt gcgtttataa acatctgtga aattactcca ttttctagca
     1921 aaatctttta agaaattttt tgtcatttca tctatttgca tttgttgcgc tgtctcgtct
     1981 tctaatgaac caatttgact gttactttca gtactttgaa gagttccact tgtttctttt
     2041 actgcttcga ttgtttcttc tgtcgttctc aattgtgttt tttgtttttc agaagaaaac
     2101 caatgacaac cagtaagacc tatcaaacat aaccaactaa atactaaaat tatccctttt
     2161 ttcacgtttc tagtcactgc ctttcttatt tcaccttacg aaccagacta cgaatttttg
     2221 tttgattgta cgtgcgttga taccgagcca taatccgtcc ttgctcggca ttctgttcca
     2281 cactagagaa atgttgtccg ccatatgaaa catctgttat aattccggtg tgaccataga
     2341 ttccaggaga caatgcacca cctgcttgcc aattcaccac atctcctgct tgtaaatcac
     2401 ttggttttgg gttcatgatt acttgccaac cataggttgt ccagttataa tcttccccta
     2461 ttaacatcgc aaacatgtgt ccagaaccca ttaactgtgg gccacctaat tgagatacat
     2521 accatgccgt accaccataa cattccccat tatttacgga tcgtccaatg atccgatcca
     2581 aaaccttcgc attgccttta ctttttcctg caataagcga agtttttttt gttggtaggc
     2641 tattccaatg cttgatcgca aaagtatcat attccgtgaa gttataggac gttatgatac
     2701 tcattgctcc tcgataatac ggactccctt cggtaatacc tgctgccgca tagcctccac
     2761 gacctagaat caataaacaa cttccaggat ctttattgcc cacacattga ttaaagttgc
     2821 tttggctcgc caccataaag ccataatagt cataacaagc ttttaagttc ggaaacttca
     2881 tatagaaacc accttcagaa ccaccaattc cacgtgcaga accttgtggg tagggacaac
     2941 caggaaacca ggtaatccca aaaaagttcg tatcgtttct tccagattgt gactgtccat
     3001 aagctgattc atagcctaat tgagaaagaa tcacactcgg taataactga tactttacag
     3061 ctgcaccaac tgctgcttgc gcatgttcat ctgtgattgg accacgtcct ttaatctctg
     3121 aaaaaatcgg taatccttcg gtatattccc cattaaaagc gacagctaga cctgtagctt
     3181 ctcctccatg ctcagaaagt tctcctgaaa aaaagaatgg caatagaaaa aagggaagaa
     3241 taaagaaaag aagacaacct agacaccctt tcttcatttc ttcctcatcc tttcttcgat
     3301 aatggttttt ggactgttcg tccttttggt aaagtaaaat cagttcctgg ttttgcgtcc
     3361 aaaatcggca gtgttttagg atccttttct attttttccg attgctctgt tggcttatct
     3421 aaagaagaaa atgcaggttg taacgatttt ggtgcggaaa tcgacggttt actttcagac
     3481 gaacgttgcc ccggttgttg cacagaacga tttcctgata gagtggctac cgacgaatta
     3541 ggaacagagt attggttcaa taactgctgc attgctggat aactcccatt tttcgcctga
     3601 tttttcactt ggcgaatctg ttgtgaaaga gaaccagcag ctgttaattg tggcacgggt
     3661 acttttccag aaggcaacgg aatcgattga ccaacaaaag cgataccttt atttaacaat
     3721 ggttttcttt gattattccc ttgttgctta ctcaattgtg ccaatgattg tacctgttgt
     3781 tttggtctta ctgaattcat ggacgaattt ggaatcaaac catttgaaag accagaattc
     3841 cactctgttt tcgatttggt agaagacatt tcaggctcta ccccatgata acgaacatga
     3901 cgaccactat aaggcaaggc ttgatttgaa cgcccaccta gttcaaaggg attaaccact
     3961 tgcctccctt ttaccgattc ttgtggtgta cgtttttgtt gaaacttttg aaatccatgt
     4021 atcgctgcag aagcgagtgg attcgttgct cgatacacat tccctgctaa tcctgcacct
     4081 ttgattgcca aattttgtcc agcagttgat agttgaccag gcagttgttt catttgagac
     4141 aaagaatgac ttgctatgtt tttcgtgttg tttatagttt gatctgcagc tcctaaccct
     4201 agtttcttta ataaatcttt ccgttttaca atgagtaaaa ttgataggag tgctttgaca
     4261 aacatatttc ctaaaaaacc aaaaacagta ttcattggaa ttaaatcgtc tactatgtta
     4321 tagatcaaaa atgccaccaa aataatcaca ctgtaaaaag atttttgaaa catcaagccc
     4381 aaaaaggact ttaaggtatt catgagcata taatcaaata ctggaacaaa agaagcgagt
     4441 aatgaaaaag gaatcacaag taataaacac aacaccaaca tttgaaagat aaaacgaatc
     4501 atgccaatca ctacaagtgg taatccaata cttagattaa gcaatggcac catagaacca
     4561 acgactgctt tataaacagc tttagttggt tgaatataga cacgataagc cttctttttc
     4621 cctctatctt ctcctgcagc agtggtcact tttgtctcaa tatcagataa ttcttcactt
     4681 gacgcttcct tggctagaaa ttcttgtggt tcaatctctg cttgatacaa ttcttctaaa
     4741 tttgttgtcc cataatttaa caatatatac ggatcaacaa cagccgcttt ataataaagt
     4801 tggcgcactt tttgaatggc ttccttttgt gtcgttggca ctaaaacacc attgacatct
     4861 tttgtcgcca attcatctag actatttgta gcagaaaaaa taaagccttc tacctccacg
     4921 gacaaatcat ttaacccttt ggtaaaagct tctcctttac taggtacgtt actaccatag
     4981 ccaaaccaaa ccattgaaaa aatgacaacg aagagaaatc gaataaacac catttttgcg
     5041 taatgtttcc ctctaaatat acgaagagta aatagatata acacatacaa gacgacaatc
     5101 cccgcaccaa taggatcgta tatttttgaa taaatagtat ccgaaaaagt gaaaaactgg
     5161 tgaattaact cgttcattgc agctccttca tagagcttct caatgacaaa atcaaggcct
     5221 tgccaaatca atttacttaa actaaagaac atattcgtca tcatttttag aaaatctgtg
     5281 ccatttgccc caaataacat tttgcctaat gaaaattcac tatcagataa ataagaagaa
     5341 aaggtatcaa tatgaaattt cccaatctca tagatcgatt gatccacctg cttttctgcc
     5401 gcatacacca atccaccaac aaaaagcagt ccgaatccaa tcgttaaaaa aaacaaaacg
     5461 caccatttct tttccttatg ctttatataa gccattgttt cacctcattt ctattgcatg
     5521 attaacgtat cctgttcttc tacttgagag tgacttgttt tttccgttgt ttgtaatgca
     5581 gcggtcattt cttcaaacaa actgtgtaca ttgagttttc ctacacgtcc atagggatct
     5641 aacatccaac actgtccttt aatcatgtgg ctcatttcag ctaagttcgc ttcatttact
     5701 tccaaacgta aatgattcaa aatcttttct cgctcactgt cttcatcaaa agcaaagacg
     5761 acaccaaaat tccctttttc tttatctgaa cctaagtcac caataaattg tgtatctaac
     5821 actaattgat tcatctggga acgccccaca cgtgccattg ctgcaataat actacgtcct
     5881 gcgttcgaat tattaaatac ccatgcttct gtaaaataaa tcgctgtttt cttactacta
     5941 tctcgttttc caaacatttc acaaaaacga cctaaactaa tcatcaaaca caaagacttt
     6001 cgatccgcat ctgtatatag ttctggtcgg acattgtcat ttggtaattt caaatcttgg
     6061 atctctaaaa tcgttgtttt ggcattaaag tctaatcccg ggttttctcc ataagaaaat
     6121 cctaaacgta aaatcgaatc ttctaccgtc aagcgtaaaa actcaccata ttctcgaacc
     6181 tctttttgtt ccatttgttc taaatagtcg accacatgca tcaagccaac ttgttgtccg
     6241 gcttcttttt gaactaaaac ttcttggatt ccctttaata ccgccccttt taaaaggtga
     6301 ttggtaccta acggacttaa ctcatcaatc atatcctggg ctacttgttt cgcatctact
     6361 cgggataaac aaacaattgg atctaataca ccataattac ggggatttgt agcatctaac
     6421 gtgacataac tcattttttg caaatgttct acaaacaaag ggtaatgttt ctgataatac
     6481 gggtcttgaa tgagtttcgt aaaccatttg cgcttttctc ctttgggatc cacaaataaa
     6541 ctttgtacat tcataaacga actatacaaa aagagcaagc caactaaaaa ggtcttccct
     6601 ttaccggttt ttccagttac tgctacatga ggcgcatcaa acaacgcacc tttgattcct
     6661 tccgcaatgg ctagaagatt caaaaagaca aaacgatttg acgcataaat taattcttct
     6721 aatggtgttt tttctcctcg agaaccacca tcccctaatg tatcttgccg accaatatac
     6781 cagccggatt ccatacctaa ttctgatact gctgaaaaca accattcgcc aactgcttct
     6841 acagttgttc ggtgcttcca atgtaactgc cgtcccaatg gttgccctaa taaacattta
     6901 tagaacaatt ctcgttgatc attttttgca cggtggatat ggataatcgt atgaagttgt
     6961 tgaataaccg ttgcactatt ttgacgaact tcttcaaccg tttgaccgta tacaataaaa
     7021 catgcgatcc aatcgacgac aggttgccca ttatctactc gttgctttaa atgttttact
     7081 aatcctcgaa cagttaataa gcgttcacta ggttcttcat cattttgaac caattcctta
     7141 tgctctgttt tgaaccgttc cgatagccaa ttcagtttta aaccaatacc tccctgtcct
     7201 ttcaagcgtg gatagtgaat tttcattcgc aattctgctg ggaaagaaag atcctgagca
     7261 aattgaaata gatcacaatt tctcatattt tcaggtgttt ccccaatagg caacaatgct
     7321 aaataactac tccctaactc atcttctaat tgaagaaggc ctttatgttc cttcggtata
     7381 cgtacatacg ttccttgatt gtctttcagt acttcccaat tattttgttc ttctactttg
     7441 tgagccattc ctcgaatata ttgcagacga ttgagataca acagctcttt ttttactaag
     7501 ggacgaatag aaaagctata aagtaattgt gtcacttctt ttacataagg atcactttca
     7561 gaaaacaaag tttccaaatc atctacttct aatccaaata agcgtgctaa acttcctgct
     7621 aaatctaacc agattagatt tattacatca tgaaaactct ccaccgttgc tttttctttg
     7681 actttgattc ctaaaataaa tgaaggacga tagacttgac cttctcgttc caacaatgtc
     7741 actgtttctt ttgcataata cgcaccaatt gcttgactat cgggagaaaa tccctttcct
     7801 aatgtgtcaa acttcgtccg taaattcatt gttcgtggat acatcaccaa ctccacatct
     7861 tcataatctg ctaaaagcaa cagtaaacga gccatcattt cttttatttc ctgtgtcttt
     7921 tttggtgtgt tttttgaaat tgttcgtgga aaaacatgat agtaggccca gatacaatcc
     7981 tctccattta acaaacaatt tttcttgaat gcttttactg gttcttttac aagcaccaat
     8041 gaatttcacc tcccaataaa aaaggaacaa ccagttgatt gtcccttttt ctaatcctct
     8101 aaataaacat acttattcac aaaaagtaat tcttccaatg cattgtttga taccttgtcc
     8161 cagttataat acacacaatc accattcatc atacgaatca tataacaaaa ccaatcccat
     8221 aaataaaaat gcactttttt tccatcaggt tttagttttg ccagtgctaa cacacataaa
     8281 taggggagtc ctatataaaa aacaactcct gctggtggaa aaaaagaaat gacccaacca
     8341 ataaatgaac gtaaaaataa atattcaaag attacgacca acaaaaaagt tgctgcattg
     8401 actaatgcaa ctggaaatgg aagacgccaa ttgcttgtaa tctgttgtac gcgataaggt
     8461 tccgcataaa caatcgaata atcataaggt tcccaatcca cttcactatc ctcctaatcc
     8521 ataaataatt tttcaatgat tttaccaaac gcatcaaata cacgtgttgg atcatttaag
     8581 aagaaataaa ctgcggcacc tgctgcgata gatattaatg catttcctac ttttgatttc
     8641 ataaaatttc gcaatcccag ataaacaacc accattatta aaaaatagcc accttgcgtt
     8701 aatataaaat ctacaattcc cttcagtgtc atttccgttc ttctcctttc ctatccaacc
     8761 aattatgtgt taatttttca acataatact gatttgtttt tttcgtaatg acaatcgtca
     8821 tttgttcttt ttgtaaaagt gacgtatcac catctcgaaa gacgaccgtt gcttttacaa
     8881 taaaaggttt tttcgtttct ttataacgca atactttcat ctctacttga tcaactttag
     8941 ctgtttgatt gagtccttca ggttctttca tcatgtatga catatctttt gcattgtttt
     9001 tcgcatactt atctaagaaa tcttccacaa atccttcaac atctttcgct tccgacgcat
     9061 caaccgtttc gtataatgtg tcatcataag taatcattcg ttgtttttct gtattctttg
     9121 gtagtgccgt aaaataagga taagcaataa tacgacagcc cttgtttgaa atagaaaagg
     9181 gaagattgag tacttgtgtg attgtcacac ttgtttcttt cttactctct ggattcatcg
     9241 agcgaatatc ataagttact ttataactag cagttggaat ggtttcaatt tcttttaatc
     9301 caataaacga agcagcagtt aattttcgaa cacctgaaac acttcctggt tcttgaataa
     9361 agttttgtgc ataaaactct ttttttaatg tttcttgacg ttgttgttct tgttcatgat
     9421 tatcgaatgt attcatatac aaagcaacaa aacgattcat aaaggattca atttcaggtg
     9481 tgtacgttaa ttctgaccga ctttgtactt ctttcttttg taattgttgg atttcatctt
     9541 tcaaaaacga cacttgaacc ttcgttttca tggattgaaa aaatgccaag ccccctgaaa
     9601 aacaaagaaa taaaaccaaa caccaaatcc atttcactgc tttttttgaa tggattttct
     9661 tttgcttcgg tactttctct gtataaactt tttctttccg ccgtagttgt cgttttttca
     9721 taagctactc cttctagtat gtttttacta gtacgtacga ttgacaacga taagcttgtt
     9781 tttctgaaaa atagtccatt caattaccct cattttctat tccttttctc tttcttttat
     9841 atccaacaaa tccagcaata cttgtcaaca ctcctcctag aacagaaagt gcaacatgta
     9901 tttcttcacc agtctttggt aagacttttg tttttccatc tgctgttttg atttcaatat
     9961 tgccacttgg aagtttcttt acaggaatcg acaaatcctt taccaaattc ccttcactat
    10021 cttggattaa tggaatacca tcttttacac caacaattgt ttgatcacct gctactttta
    10081 atggggtggt tggtgttgga atatgggtca attcctctac attactagtg acaatcggta
    10141 cactcattcc ttgagaagta ttcgtcgtaa tttcaccgct tggggttacc gatactgtga
    10201 cttcttttgc tgaaacaata ggtttatttt cttttggaat tgcttctgat ttttcaacag
    10261 gttgtttcgg ttcttctttt tgttcttcac tggtagacga catcgttggc tgttccgtac
    10321 ttgacgaatc agtattcttt tcagaagaac tggttgttcc tgattgttcc ggagttgtga
    10381 ctatacttga atccgttgtt gttgaactat gcacaccttc ttcttcgatc gaatcagtcg
    10441 gtaaagttga ttcaggaaac tctggttctg gtgtcactgg ctctttttca ggtttctctg
    10501 actgcaattg ttcaggttct gctggttttt ccgttgttcc tccatttagc tctgtctcaa
    10561 catctggtaa tccttcccct ggcaatgttt ctacaatttc tgacttaggt gtttctacag
    10621 tttcatctgc ataaaccaat gaactcccta attgaaacga aaataatgaa attcctagtg
    10681 ttgttacgac tgctaatgtt gtttttttca tgattattcc tccaattttt tatgcaattc
    10741 gtgttttgtt catagacgcc gcactaatat tatctgaacg ataaaagaac tcccgtccgt
    10801 tttcttctaa cccaacatat ggattatttt tgaataattg aatcaatagt tcactggtgt
    10861 tgtattttgg atagtttgca aaaaaataat actgtgcttg tcgcccatcc gaaaattcaa
    10921 caagtaattc ataacgatac cgcttcaatc cttttcgttg ttttaaagga tctctatacc
    10981 aacttgtcca tcgttccatt cgtggataga gatagacacg atacacatag aacaatgcca
    11041 taccacatac caccaaaaat aataatcgcc acataaaaat tccccctttt cttctacctt
    11101 tcttgaatca tcgaacattc ataccctcta tagagatttg aaaaatccat catcaattgt
    11161 tcatataaac gttgttgcaa atagcgattg tttcgaatac ataattcttc atagccatca
    11221 atcacttgat cttctttttc atagcatctt gtagctctat gacgctcagc ttgcaagaca
    11281 taaccaaact ctgcaccata tcgttctact gttaataact gttgactacg caataatgca
    11341 atataacaat cacaaaaatg ttcaaccgac atttgatttt tctcacgttt gtgaaatagg
    11401 tattctttag aaagcacatt attcgttaaa caatctgata aatagcgata atctggcggt
    11461 cggttatttt catctgtgaa tttttcccat aataaactga tttttattcg ttcagcggct
    11521 agatagtctt gttcgcgtgg ataaaattca tcaatcgcac aaagtttttt atcacctgaa
    11581 taaacaaatc catataatgc gaaacgaact cttccttttc aattggctct cctaaaaatt
    11641 ccagtgacat accatcttct ccttcctaaa aaaaataaaa aagcagaaac cgatttggct
    11701 tctacttttc tttcctttta tagctctttt ttacggaaga atccgcaaat gacatttttt
    11761 caaaaaatca atcggcacaa gttcacgaaa acttggagca attttggata gaatcacttc
    11821 cccatcttgt aaaatacaat cataaaaatc atcagttact tgatacttac gcttcttaca
    11881 ataattgata aaatagtagc gatcattttc tatttccatt cgttgttcta ctaagcgcat
    11941 acgaacatca tcatagaaat aagccacaaa tcgcccttca agttcatgtt tttcaatggg
    12001 atactcttct ggtaacaatc tattttttcg tcgtttatac attgctggat accttagaag
    12061 aagttcatct aaacgtctgt cagtatatgg taactgacga atcaacgatt ctaatcgttc
    12121 tttatacaga tagaataatt ggcgcaagca atagtgtacc gcctctggat tttctaatac
    12181 tgttggatca cgtgaccaat acgcaccaac ataaatcata atttttttca tttagacaat
    12241 cacaccagca atactaatga ccgatccaca ataggtagaa caagtggcat agcgaccaat
    12301 ttcctctaat tctatctcaa tctcttctaa agaaagatat gtctgttctt ctgttggctg
    12361 gcgactcatt tcccaagtga cagtcaccaa tgaaatttct tccataagag gtttttcgct
    12421 gctaagtgct gccacacagt tttcatatat cttcatttta tcgcagcctt ttttaaccgc
    12481 taaaatcgca ggtattggca atgaaaatcg ataaaagcga atgaattcta gccttccttt
    12541 ccttcggcta aattccaact cttcgagttc ttgttcgtat cccttacgaa cttctaataa
    12601 gcgtgcaatt tcttgccagt tctctttttc ctgataacgt ccagctttta ccgcttgttc
    12661 aatataagga actcgaaaac gaaaataaga caaatcaacc atcttttcat ttaatgcttc
    12721 gattgcagcc tttaaatcca acaggcgcag ccattctgtt gtcgtatatg cattcatgca
    12781 attttccctc ctgacgttcc catccattct agatagtttt gttgctcgtc atttagttcg
    12841 gcattatcaa tcccttcttg tacaaagtct cttcctaaaa tacgatctgc ttcttgtaac
    12901 aactttaatg tcggagctac ttgtttcaaa aaccagcgtt ctgaacgatc taatgagaat
    12961 ttttctggct ttactgtaaa atccaattct tccatcgaac gaaataattt tgcccatcgt
    13021 ttatcaacaa tccgtgcatc actttccagt ctatcccaaa caataagttt tgcattaatt
    13081 aaaccaacgc caatctctcc taaacgtttc ggtgaatcac ctaaaataaa tgcttctact
    13141 gcttgtgttg ctttctcatt ggctaaccgt aactcatatc gattataaat accatatttc
    13201 gttaatgctt ctaattctga aattttttct cggttcgcaa tttcaaaacg tttctcataa
    13261 aaattaaaaa agagaggact ctttcgcgat ccaaaataca aagataatcc ttcacttcga
    13321 aaatagccat catctaaaaa acaaccgcca atattctgga atctcttaaa gtccatttct
    13381 aataaacctg ctttttgttt ttctagtaat tcaaagagat cgaaatgttc ttccgtttcc
    13441 aatccccaaa gttcatctaa cgcaatatcg aatcggctac attgcacacg tgtataacca
    13501 gcttccaact ttccttgatt tttccaaatg gaatatagcc atgttcgcca atctgtttgt
    13561 tgttcacgca tgacttcctc atattcccgg caaccttgtc ctgaaaattg cacaagtaca
    13621 ttttctgtta cagttcgttt tgaatgccag aacacccaga tattcccacg aacagaaaga
    13681 cagtcaaaaa gatacaagcc cgttggacgt gtttcaaatt ctgtcagatc cattcgtaat
    13741 acacgttgaa tcactgtttt gtagtctgtc ccaaaaaagg aaagacgcaa ataatcaatc
    13801 attgctcgaa gatctattcc gcttttcata tacaatcgaa agcgttcctt gattcgttgt
    13861 tcaaaatcct ctgggatttt tcttttccct tgttccatct ttctaatgta atcttttgag
    13921 cagcccatca attgcgcaaa tcttgcttgt gatagcggct ttttatctcc acttatatta
    13981 tgaattgcac ttaacttctt tctgtagctt tttagctcaa tcggtgtcat tttctcgctc
    14041 cttttataaa ataacaaaat cgattttttt aaccctttag aaacaccgat atatcaacta
    14101 gtccccctcg ttttttggga aaagtagtac aactgctgac cttagataat cacttgatac
    14161 aaaagggttt ataaaacttt tttctatgtg ttatgttatt tctgcccccc atattatata
    14221 tcggggggct ttacacaaag aggccgtgcg caagcgcacg acctctttgt gagctgcaca
    14281 cgctgctagc gagtgcgcat tataggccca tgcttcgcag ccttaatgcg caccgccagg
    14341 cagagtgcgg ctaccagact acgtctagag ccctcccgat agataataac tacaaaatta
    14401 aatgatctaa ttttgttatt tcactatatt tttcaggaga aaaaacgcga taaattgaca
    14461 aagttaaatc ctcttctaaa tctactcgat aatgatacaa tcgagtcaat cgttctatat
    14521 cttttttcgt tagcaatact tgattaataa cttttcctgt aaaaacacgt tctttgcgca
    14581 aaaagcggta atcagttact tgttcaatac gattcaacca atcataaaac gttctggaag
    14641 aaagagaagg attaatcatt cgtattgctg tagctactct ataataacaa ctctctgcca
    14701 tttaacttca cctccttagt tgattacaaa atatgcgcat cagaaaaact tgttcctttt
    14761 ggatcttttg ctaaatacat ttggcgctct agttgttctt gtaacaactg attatcatct
    14821 cccatcgttt gatcttgact atcctctttt actgtaaaat catagacaat catttcagta
    14881 aatttctcaa attctttgat aaagtgataa tctttgtaaa caaatggcgc aaaaaactct
    14941 acaggtacat caaatcctgg aaaagcatat ccacgaccag gttgatcttt gttgtaaaat
    15001 ggcttatttt taaaatcttc tccaaaagtc attaaataac caatgggaga caatcgacca
    15061 acagaaatac ggaatgttaa attatcccga acaccacttg gaaaatattc tgcatctggt
    15121 cgttgcattg ctaaaataat gtacatccct gcttgacgac cctttaatac taatggctga
    15181 atcaatttat cgactctatt tctcgcttcc atatccaacg aaccataaaa agctgcccat
    15241 tcatcaaaaa agataaaatg cggtggtaac ccataataag cataattttt ccctggttca
    15301 tatttaggat ctttcatggt tttcatatag gcataccgtt gattcatttc ttttaatgca
    15361 tttcgtaaac aaataatcat ttcttcattt attttgtaaa acacatgtcc tttgaataca
    15421 tcgacgtcac ctaatgctgc taaatctgcc gcttttccat cgcaaatatc tactgtccca
    15481 acacttaaac aagcttgaat taaagaatac aataaatagg ttttcccacc accgattgca
    15541 ccagcaatca acatatttgg tgattgatca tatacccaat ccaaagtttt cattaaatga
    15601 atacgtccat tttctgctat acattgatca ataggaatcc gaccagaatc tgaaaggaat
    15661 acataattga ttcgggcttt ttctcgttct tcacgaatta aatcacgcaa aaatacttca
    15721 gataacaaat gacccatttt taaaaatcgt tcgtgaaaac gcccaccatc taaaggaaaa
    15781 gaaaccacta attcttgttt cttttgataa aaataaactt ttggtaatct cattttttcc
    15841 cttgtttgtt tctcactaac ttgtttcttt gtataaacaa accgattgtc aattaacatt
    15901 cgaacaagta actgacgcca ataaacagag gcaaaatatc cttcttttag tcgcgaccaa
    15961 tgcatcaacc ctaaaattaa cccactaacc agcaacaaat acaacacaat acttattcca
    16021 ggaataagat acatttgcca attcgaaaac gtacttaaac gtggataaaa tactttccaa
    16081 ctaaaccaac ctaacagaat caaaaatgga aacaaaaaga aaccataata gcaaaaccat
    16141 aaataacgat tgaaatagcg aattcgccga ccacgatact tgaaaaaatg agctttcaat
    16201 tgtcacattc ctcctttctg tttttacttt tcctgtgcta aaaacttttg acaaaaagca
    16261 actaatacag gaatatcaca tttagtaaaa aaacataatc gaaaaagatt taatcgattt
    16321 ttataagggt gtgcacacat attacatatt cgtactatac gaatccaaat cacttcctga
    16381 tgatcttgtt tccactggta cggtaatgaa aaacgaacaa gatcacgtaa aaacatacac
    16441 tgcccaataa ccgcacaaat atatgcattt gtttgtacaa aaatcgttgg attgtctcca
    16501 aaatactcaa tcaattgttg gatattttga taacaagcac aatctctcaa agtatttgat
    16561 tggttttatc tgaattcaaa atctcccctt ccttctatct tagataaaaa gaataggaag
    16621 actccttcct attctttcaa aaaaacctta ttttttctct ttttccttag aatcaacact
    16681 tggttgtttt tgatctaaac ctttttcttg tgattgtttc atattaggat tatttgttgg
    16741 agcttctact ttctctaatc ctttcgcata aatcactaaa tctgaatatt cacgattgcc
    16801 atcttgtgct ggaatatata ccacatgata ttctggatca attaatttca caggatcacg
    16861 tcgtttaaaa ccagtcaaat ctacatctgc tggaaattta actcgtcgtg caatctttgg
    16921 ttcatctgga tttccaaaaa ctaattgtgc tcgctgtgat cgaatttctc ccgtataatc
    16981 gcctgtttca ttatcaaata cacgatcaga ctcttctaat gcgccaaagc gtacggctcc
    17041 taaacaagtt tcatctaatg cttctgtaat gtcataatta tttggaaatt ttaatcccat
    17101 cttacaaaac ctccccaatt tttacaccag ttaaaaagac accaacatct tgatagccag
    17161 ttgttcgacg ctctgtttgt cccatatcat ttgtttcacg actattcact cgttctttac
    17221 gaaaaacaaa ataagcttct tcaaatgaaa tacgcacctc tttttctggt aatcgtaatc
    17281 ttttatcctc ttcagctgtg agataaacaa aaaaagcagg ctgttcttct cgcgaacact
    17341 ctgcttgtaa ttgaacatat cgcccagaca caccattaat ttcctttggt ggattaggtt
    17401 cccgtgaacc caataaaaat acttgaccta aaaactcttt cttcatacgc aatgcttttt
    17461 ctgccataaa gtttttcctc ctcttaaaaa ccaaaataaa ttctaaatat ttctacatta
    17521 caatcaaaca agcggttaaa aatcctaacc gcttgttcct catgactaat ctttggcttt
    17581 acgtacttta taaagtgtta tacctaaagc aatgactaat actactaatc caagaaccat
    17641 aagtatacta cttgtttgtt cacctgtttt tggcaacatt ggtacaacag tagctggttt
    17701 aacccgttgt tctggtttca ctggttgcgg tggttttggc gcttccacaa tttgaaccgt
    17761 ttgatctttg tttgttagat ctacattgtg tttcgccact tcttcaaacg tttctttctc
    17821 atcttccgtt ttcgcttgga gccattccgc aaaactcaag ccttttcctg caagattgcg
    17881 accgtccact aagaattcaa ataagaattc catcatttct ttatcggccg caaagacttt
    17941 ttccgcatga gtgccttcaa atggtgtgcc gtctggtgtt aagtagcctt gaacaattaa
    18001 gcgatagatt tgaccaatcg caagaccgtc tactttgacc cattcaagca cagtttgatt
    18061 tggcaaggcc aagatctctt ttccgccatt tttattcgtc gctttcgtcg taatggttgg
    18121 attactgatt ttgatggttt ggtcattgtc ggtaatatct gcgtgagaag ccaataatac
    18181 gccttctcta aacagatctt caaagaccac gacttctttg cctcgcaacg cacgtgcgtc
    18241 tagaataaag tctagattga ctgtaccatc cgttgtttct gccacaaacg ttaaggtact
    18301 tgtgacttct tttccatcta ctagcactgg tttttccgtt actttgtcca ttagaacacc
    18361 gttgactgtg tatgtttttc caacgaccaa atcgtggtat ttcacagcgt ctgtaatggt
    18421 gactttcgtt aatggattaa cctctttcaa gccagtgggt ttatgtgtag ccgttgtacg
    18481 tacacttggg ttagttactc gtaccgtttg ccctttgtcg ttgatgtctt tatggtttgc
    18541 tagttcttca tcatttctaa aaagactttc aaacacgacg atttcacgac catttatacc
    18601 tgtcaaatca aaggtaaatg gtacgatcac cgtttcattt tctgctttgg caataaagcc
    18661 aacactcgtt tctaccgtat ttccatgcac aagcaagggt tcaccggttg ctttatccat
    18721 taagatccct ttgaccaagt atgcttttcc tggaagaatc tcggtaattt caacggtatc
    18781 gtttaaggta aacaatcctg ttgggtctgc gactgtcaat ccgtcttcaa agacagccgt
    18841 ggtatgaacg gttggattat tcactttcac actttgggct tcatcgttga tatccgcatg
    18901 tgctgcaact tcaatgccat cacggaaaag tttttcaaaa acaacgatcg tttgaccttt
    18961 cagaacacta gcattaacca caaattcaag gtcttttgtg ccattgactt tgtctgcaat
    19021 aaaggccaag gtacttgtga tttctttgcc atcttgtaaa attggcgcat tcgttgcctt
    19081 gttcatcaat actcctgaaa tggtgtattc ttttccgaca aacaagttgg tataagaaac
    19141 actgtcttta atgacgatcg tttctaatgg atcaaccact ttttcaccat cggctttatt
    19201 tgacgcttgt gtttctagtt ttggtggaat caagtaatta cgtacagaaa ccactaacgt
    19261 ttccccatca tctggcgcat tttgtttgaa tgcaaccgtg aatgcttgtt tatctgtatt
    19321 tggttggaaa ctttctaatg gggcattttc caagaagtaa tagtcatcgg caactagacc
    19381 tttcactaaa atttccccgt tttgatcagt ggtatattca ccaattgctt gatttttacc
    19441 gttatacaag gtaaagttca cacctgcgag cggcgttttc ttcttgttcc attcttcata
    19501 cttgaataat tttacacttt tccgtgcttg acggttcgca aaatggatca ctttgatcgg
    19561 ttctgtatca ttggtaatcg agaacactaa tgggttttga ttcaaaacgt aatttacggg
    19621 ggctttgatt tcttccaaac gatagcgatc ttttccataa gtaagtgtac cgttagttag
    19681 gaaaaagcct ttttcattgg ttttaaagat atccgttgtt tctgtatcat ttggaatctc
    19741 cattgtgaca taacgattgg ctaaagtatc aaaaatcttg aattctgtat tagcaagtgg
    19801 aatcactttt cctgtttctt gatccacttt caccacttta agacgttgtt caattacttt
    19861 atcttctaaa acataatgga aagtttgccc attttcagaa attgtgactt caagtggttc
    19921 aaatagttta tacccttctt ttcctttggt ttcagaaaga atgtacgtat caaagacaac
    19981 ggtattttta ccaaaagtag cataaccatc attgtctgtc acaacggttt tcaccacttt
    20041 gccagttgtt tttgacgtca aagtgatttc aatgccagct aattttggtt tgttatctgg
    20101 attcgttgaa tcgactaaat ctttattagc aactttcact aaatcaatgg ttcctttgat
    20161 cacttgatct tttgcggtcg cattgacagt ttttccaggt tcaacaacaa tttcaattgc
    20221 ttcatcagaa agcacataac cttttggcgc tttgatttct tttaaaacat atttgccagg
    20281 atcaagatct ttaatttctg cctgtacttt gccgttaatt tttttcaagg taatttcttt
    20341 aatctttttc ccatctgctt gatacaaacc atagaccgca ttttctaaag aagctgcacc
    20401 ttgtgctttc acaccagttt ccgcatcttc tttaaacaca cgaatgtttc cttgttttag
    20461 aatttgtaaa ttaataacag acgcattagg ttctgttgga tgtaacacac caattgtttg
    20521 agctcctgga cgacgatagg ctaatggcgc accttgaact ttaattttat tcaattgaat
    20581 ttttgcttta tccggtgcct cttttgtgac tgtaatccat acttcattgc cgtgaataga
    20641 tacattgaca ccttttggtt tttgaatgtc tttcacatat ttcaaaaaat catttttatt
    20701 ctttactttc aacgtttcgc caatttttac cgtatgtgtt tgattattaa aagttgcttt
    20761 ttcaccaaag ttattaatca aacgttgaat ttctgctttt cgacgatcat aatcaacacg
    20821 gatatttttg attgggattt ttgtgcccaa agcttcccaa atcataaatt gtgccgcaat
    20881 gtattgttca tcggatttgt ctttatttgt gacatatcct aaataagaga tcaagcttgt
    20941 ttttttccgt acatttgaat ttttcaaata gtttttcaaa gcagtagctg tataaacttg
    21001 accttctgcg gctgagacac caggctcaat acaaaacgcc acttgtccat taatctcaaa
    21061 acgaaccata taatttaaag tgaagtttag attgttcgga ctagaagtaa caccaatatt
    21121 ttcgccccaa tcaattttcc gaccacctaa aacagctgca ttggttgcac ggcgaacacg
    21181 aaccgaacga ttggttggta ctgttactaa cagtgtaggt gtacctgttt ttggaatgtt
    21241 ctgctctttt ttcttttgat tttcaatact agccgtagat tctttgtttg gtttcgttgg
    21301 ttgattttga ctagctgcgg ttgaagagat tgttttttca acgttttcct cattttttcc
    21361 ttctttgtca gcagtagccg ctggttgttc cactgtttct tctattcgtt tcttttcttc
    21421 cgcttgtttc tcagaagact ctggctgcac agaaggcgtt gcttcggcag ctttttctcc
    21481 ttctagagtg gacgtttctg aagcaggggt tgtaattgag gcagtttctt caactggttg
    21541 tgaagtacca ggactattcc ctgggtgttg tgtttcccca atcgtctgat cgagagtcgt
    21601 tttggaattg accgttggcg cttctgtcac aggggcctgt tcaacagtgg cttcaccagc
    21661 tgggaccccg gcttctgctg gttggacttc tgtcacttca gaagcgactt gggcaatggc
    21721 ttccatcgta ggttgtatag ccgtcgtttc ttctgcatgt gcttgagcag ttcccaccaa
    21781 gaaacagaat tgagaaaaag ccaccaatgc gagtgctttc aaccaattca ttttcgtttt
    21841 tgcatacaca agaattcctc cttttcctga gaattttata taataaaaca aaacgccgtt
    21901 accaaaagtg ataacgacgt gaactgtctc ttctattttt atctggcgct cttatctgaa
    21961 cacatttcat caatgatttt accctcccac tataattaca gaacaaaaaa agaacctgct
    22021 tcgtggaaat caactgtcca tttcccaatt ggaataccag cttttttggc aacttcacca
    22081 tcaccaaatc tcatgccata ccaattacta ccttcatcct taatttcttt ccaaccatat
    22141 gacatagctt catctaatga atcaaaaatc atgacactat tgccaattgt tacaggatct
    22201 ggcttaagat cttctggaag agaaatatca atcataccat aatgatccga ttcctcagta
    22261 ttattgtcat tactagctac tggtttggtt gttctactgg cttattttga tacaacaagt
    22321 actcttattc ttcttttact tctccgtttt caatttccca ttcaaaccac tccgcaaagt
    22381 cttgcttaaa accatctact cgatacctat tttgtaaata ggcatcttct gacgtcgtag
    22441 gatctaaata ggtaggttta ttctctggac gagcatagac acctacccat gtttcgtaat
    22501 ctccttcaaa ataactatct accaaatagc ctttttttct aagaatctcc cgaacttgat
    22561 ttttaattat tgatacttgt tcaaaaccat cactcattct atcacctcaa ataactctag
    22621 attctcataa atattcccga caatttcaat cctatcaatg atctctaaca aatcgctact
    22681 tatgcttccc cattcgtata agaatttccc ttccgcaaaa taaattttcc ataaacttct
    22741 tcatgatcgt cccaaccaat atcactctcg aaaaattcca cgttgttctt atcttttagc
    22801 tcagtaaatg ccataagttc aacatgtttt ccaacctcga aacaatcaca ccacccatca
    22861 accgttacac gtgtgccttc aatacctaag aaactttgtc ctcgtgtatt aaacactaaa
    22921 actttccgca ttttttgctc attaattaat cacgcgcgaa atctaggaat cacaacttat
    22981 tcgcttcttc tttataaata aaaaaactcc aataatcgga aattactatc ttttcctcat
    23041 tcctttttta aatcttca
//
LOCUS       DAYYAG010000034        22739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000034 DAYYAG010000000
VERSION     DAYYAG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.034"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(487..2379)
                     /locus_tag="ACHARQ_001168"
     CDS             complement(487..2379)
                     /locus_tag="ACHARQ_001168"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409602.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2376..4103)
                     /locus_tag="ACHARQ_001169"
     CDS             complement(2376..4103)
                     /locus_tag="ACHARQ_001169"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409603.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4119..4769)
                     /locus_tag="ACHARQ_001170"
     CDS             complement(4119..4769)
                     /locus_tag="ACHARQ_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8409604.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4973..5269
                     /locus_tag="ACHARQ_001171"
     CDS             4973..5269
                     /locus_tag="ACHARQ_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8409605.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5461..6081
                     /locus_tag="ACHARQ_001172"
     CDS             5461..6081
                     /locus_tag="ACHARQ_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8409606.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6173..7339)
                     /locus_tag="ACHARQ_001173"
     CDS             complement(6173..7339)
                     /locus_tag="ACHARQ_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8409607.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7351..7704)
                     /locus_tag="ACHARQ_001174"
     CDS             complement(7351..7704)
                     /locus_tag="ACHARQ_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8409608.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7832..8854
                     /locus_tag="ACHARQ_001175"
     CDS             7832..8854
                     /locus_tag="ACHARQ_001175"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8409609.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8873..9034
                     /locus_tag="ACHARQ_001176"
     CDS             8873..9034
                     /locus_tag="ACHARQ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409610.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9293..9727)
                     /locus_tag="ACHARQ_001177"
     CDS             complement(9293..9727)
                     /locus_tag="ACHARQ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409611.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9837..11696)
                     /locus_tag="ACHARQ_001178"
     CDS             complement(9837..11696)
                     /locus_tag="ACHARQ_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8409612.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11697..11837
                     /locus_tag="ACHARQ_001179"
     CDS             11697..11837
                     /locus_tag="ACHARQ_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8409613.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11814..12978)
                     /locus_tag="ACHARQ_001180"
                     /pseudo
     CDS             complement(11814..12978)
                     /locus_tag="ACHARQ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13285..14709)
                     /gene="araA"
                     /locus_tag="ACHARQ_001181"
     CDS             complement(13285..14709)
                     /gene="araA"
                     /locus_tag="ACHARQ_001181"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8409614.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14725..15423)
                     /locus_tag="ACHARQ_001182"
     CDS             complement(14725..15423)
                     /locus_tag="ACHARQ_001182"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8409615.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15380..17041)
                     /locus_tag="ACHARQ_001183"
     CDS             complement(15380..17041)
                     /locus_tag="ACHARQ_001183"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8409616.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17259..18659
                     /locus_tag="ACHARQ_001184"
     CDS             17259..18659
                     /locus_tag="ACHARQ_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8409617.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18911..19990)
                     /locus_tag="ACHARQ_001185"
     CDS             complement(18911..19990)
                     /locus_tag="ACHARQ_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8409618.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20076..21035)
                     /locus_tag="ACHARQ_001186"
     CDS             complement(20076..21035)
                     /locus_tag="ACHARQ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8409619.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21047..22558)
                     /locus_tag="ACHARQ_001187"
     CDS             complement(21047..22558)
                     /locus_tag="ACHARQ_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8409620.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
BASE COUNT         6472 a         4803 c         4043 g         7421 t
ORIGIN      
        1 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
       61 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca
      121 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttaccaagag
      181 gttgtgagcc tgccgtttaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca
      241 ggtttgttga ctattgtgac ttgtgaccga ggagttggca ggagaacacc gtttaaggaa
      301 gaggttgtaa aatcagcggt ttgacctgag tattagagat acaaaacgga aaaatagctt
      361 ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac accgtttatt
      421 cggaaaaagg gactgtgaca cgacttttgt cacagcccct aaaaaaaata ataaatttgt
      481 actattctat gaagcttctt cttcagaaaa ctgactgtta taaagatctg catagaatcc
      541 gtctttttcc atcaatgtat cgtggtttcc tgtttctacg atcgaaccat gattcatcac
      601 aataatgttg tctgcatcgc gtattgtaga caagcggtga gcaacaacga aacttgtccg
      661 gttttccaat aggcggttca tcgctttttg aatcagtatt tctgttcgtg tatctacact
      721 agatgttgct tcatcaagga tcaggacatc tggatttgct aagaatgccc gcgcaattgt
      781 gattagctga cgctgccctt gcgaaatatt gcttgcatcc tcgtttaata cagtgttata
      841 tccttctggc aatttacgaa caaaatcgtc tacgtgagca gctttagcag cctcaatgat
      901 ttgttcgtct gttgcatcgt cgttcccata tttgatattg tcatagatac ttcctgtaaa
      961 cagccaagta tcttgcagta ccattgaaaa ctgtgcgcgc aattcatcac gtgaaagatc
     1021 acgggtatct acaccatcgt aacgaatact acctccagaa acatcgtaga accgttcaag
     1081 aaggttgatc agtgtcgtct tccctgcacc agtcggacct acaatagcga ccatctcacc
     1141 aggtttgaca tccaagttaa aatcagtcat tagcaagtta tctggtgtgt agccgaattg
     1201 aacattttca aaacgaactt tgtatggtgt atctttttct tctggaaggc cagatttttc
     1261 attggacatc tcttcttcat ccaacacttc aaatacccgt tcggctgaag caactgttga
     1321 ttggatagtg ttcatcaaac ttgcaagttg agtgattggt tgagagaact gtgttgtata
     1381 ttgcaagaac gcttgaacat cccctaatgt catgttccca tttgcaactt ttactccgcc
     1441 tagtacagcg acgaacacat agcctagatt tttaacgaaa ttcatcaacg gcatgatgat
     1501 tgccgaaata aattgagctt tccaaccagc ggcatacaga ttctgatttt ctttttcaaa
     1561 tacttcctgg tctttttcag tatgattgaa tgttttaaca atggtatgac caccatatgt
     1621 ttcttctact tggttattca ataaacctaa acttttttgc tgatctgcga aaaacacttg
     1681 tgatttcggc gctacgatca tcacgatgat caagcttaaa gggatcatca ataacgcgac
     1741 taacgtcaat tgccagctga ttgaaagcat catcacgatg accccaatca atgtgactgc
     1801 actagttaca aattgagtca aattttgctg cagcgtgctt gcgatgttat ccatatcatt
     1861 gattgctcga gacatgatat ccccatttgt atgtgtatca taataggaaa tcggtacacg
     1921 attcattttg gcttccaaat catgacgtaa ttcataaact gttcgttggg aaacacgtgt
     1981 cataataaat tgctgtaaaa agttgaatac agcagaaatc aaatacatca cgattactgt
     2041 cgcaatgatc tgcgcgattt tatcaaagtc tatcggaaat tttgttactt gttggccttg
     2101 ttgcatctgt tgagcgccag ccattacacc tttgaatatt tctgtggttg ctttccccaa
     2161 gacctttggt gtttggattt ggaagatcac ggctgctatt gctagtacaa atacagcgat
     2221 gatcgcaacc atacgtttag acatatagcc aaacaatcgg cgtaccgttc cccagaaatt
     2281 tttaggcttt tcacctttta caccgagatt tctgccaggt ccaccatgtc ctggaccgcc
     2341 tgctggtcca tgagagggac gtgtgtttgt tttactcatg ctaagtcctc ctctcttagt
     2401 tgtgaatgaa cgatttcttg ataagtttcg tttgttgcta atagttcttc atgtgttcct
     2461 ttgccaacca gttttccttc atctagcaca aggatcatat ctgcatccat tactgtactt
     2521 actcgttgag caactaaaac ggtgatactt tctttcatat tcgttttcaa tgcttggcga
     2581 agattggcat ccgttttgaa atccaacgct gagaaggaat catcaaacac atagatatct
     2641 gctgttttga ctaatgcccg tgcaattgcc agacgctgtc tttgtccacc agagaagttt
     2701 cctcctcctt gttccacatg gctatctaag ccatcttcca tctctgaaac aaaatcttta
     2761 gcttgtgcga tttctaatgc tttccaaatc atttcatctg ttgcatccgg cttaccatac
     2821 tgcatattgt ctcggatcgt tccagagaac agaactgctt tttgaggaac aaagcccatt
     2881 aattctcgca aattatattg agtcatgtca ctaatgtccg ttccatttat ttggatcgaa
     2941 ccagattcga tgtcatataa tcgagggatc aaattgacta aggtcgtttt acctgaacca
     3001 gtccccccta tgattgcgac gatctcgccc gacttggctt ggaaatcaat atcttccaat
     3061 gcaagttttt ctgctccatg atatcgataa ttgacatgat tgaaggccaa tgttgcgttt
     3121 tttccagcaa aagaaactgt tttaggattt tcagggtcat ggataccgat tttttcatcc
     3181 aacacttcat taatacggtc agctgaagct tgtgcacgag gcaccatgat aaaaatagcg
     3241 gaaagcatca taaagctgat caagatctgc atcgcataag tcataaatgc aatcagatta
     3301 ccgacttcca aagtcatatc tgctatgtag tgtccaccaa accaagtgat tgctacgttt
     3361 gtaccgctca tgatcaatgt catcattggc atcatcaatg cgacgatcgt attaactttg
     3421 attgccgtat ttgtataatc tttattcgct aaatcaaaac gattttcttc aaagcgtgtc
     3481 ttatcgaatg ctcgaatcac tctgacccct gtcaatcctt ctcgaaatac caggtttaaa
     3541 cggtcagttt tcttttgcat acttttgaat aatggaacag caaagtacat gattccgccg
     3601 atcaaaataa tcattactgg gatgacaact aagaagattt ttgtcaactg atgatctttt
     3661 tgataagcaa gtatgctcgc gccaatcaac gtgattggtg cattgatcat catacgtaaa
     3721 aacatttgtg taaccatttg gatttgagtc acatcattgg tcgttcttgt gatcaaagag
     3781 gccgttccat atttatcaaa cgcatctttt gtcagactct ctgatttttt atagacatcc
     3841 gtacgcaatt gcttacccag tttctgtgat tcacgagtag caaaaaatgt atttgcgata
     3901 gcagctaaga tactgatcaa agaaaagccg atcatcacaa aacctgtatg ccaaatataa
     3961 tcgacatctc cttgcgctac ccctttatca ataatattgg aggtaagtgt cggcaaatat
     4021 aaatctgcca gtacttggat caccatgaaa actgcagctg caattgctga agtcaaggag
     4081 atccgtttta cgatttttag catctagttc ccttctttct atttatatac tccgtttttc
     4141 agccaagaaa gtttcaaatt gaattcactg attgcttgct ctactgagta atcttcagat
     4201 gttgaaaata agcgatacga atgcgcaatt gttgtaaata cagtattcat cactaattgg
     4261 atcagtaatt ccaattcatg atcatttttt actttcagct tcgtccgatc gatcaattca
     4321 agcaactctt ttgcatcttg tttgtgttct ttatgtggat cgtgaggcgt tttttggcga
     4381 ctcgtatgag gagcgacttt tcttgaggca cggtaatcca tgttcataaa caagttacga
     4441 taaaaagaag cattctcgcc ttccaaaatc tcataaatca ttttagaaaa ataggattcg
     4501 aatcctttga atagatcacc atcggattct ttcattagtt gtaacatatc tttagagctg
     4561 tcacgtctca ttgtctcaaa gtaataaaaa taaagatcct ctttatcttc aaaatactga
     4621 tagaaactgc ctctagggat atccgctaat ttgacgatat tagcaatcga agcttctttt
     4681 aagggtactc tagaaaactc gattctcgca gcctcgatta gtcttttttg tttttcttta
     4741 ggcaaatgga aaaatgtctg tgtcggcata tggattctcc tttctttcaa gatgacacat
     4801 tgtcatatga cgctatgtca ttataacacc tcctacctgt tttgcaagac atttgcatag
     4861 tttttttcat tatcatccga tacattaaaa aagtgataag aggaatcctt ccatttgaat
     4921 taatgagagt tgcccattaa aatagccaca tactaaacaa aggatgagat aaatggccca
     4981 atcaattagt gtccaagact tcaataaatt gcctctcgat gctgacagca tcgttttaga
     5041 tacaagaaat accttagatt acacaaaaaa ccacttaagc cactcgcttt ctatccccgc
     5101 tgctatcttg ccaagacaat taaaaaatct agatccaaag aaactttatt atattctaag
     5161 ctatacaggc aggcgatcag aagcattagc cgatcaactt actgctaatg gttttcgtgc
     5221 tgtttatatc attggtggga tggaagctct tcagtcgagt gttgcttgaa tattgcttgt
     5281 tagattcaaa agaggctgtg acaaaaaatc gtgtcacagc ctcttttata ccctgtcttt
     5341 ttttagttat ttattcttca accttatcaa tatgacttga tagccttttc tttttcgagt
     5401 aaactgattt tgttagtcat agttataaca atcatagcaa taaaaggaga aggtaagcat
     5461 atggcatctg ttttagtagt caaaggtcat cccctaactg cagaagaatc gcgcacagtt
     5521 aaggcattga cgtctttttt aacaagttat aaagaaaatc atccagatga tgaagtgact
     5581 gttttggaac tttatcgcga tgatattccg gaaatcgatg aagaattatt atcaggttgg
     5641 gaagcattac gtgctggtgc agaattcact tccttatcag aaagtcaaca acaaaaaatt
     5701 gctcgtttca atgaattgac tgatcagttc cttgctgctg ataaaatcgt tatcgcaaac
     5761 gccttatgga atctaaatat cccaacaaaa ttaaaagctt ggttcgatac cgtcaatgta
     5821 gctggaaaaa cgttccgcta tacagaaaac gggccagaag gattagtgac aggtaaaaaa
     5881 gcactgcaca tccaatctaa tggcggtgta tacaacggac aagactttgc ctctcaatat
     5941 gtaaaaggta ttttaaactt tgtaggcatc gatcaagtag atcagctatt catcgaagga
     6001 atcgattatg accctgaccg tgcagatgag ttgatgcaaa atgcattaga taaagctgct
     6061 gctttaggta aatcatttta aaaattgaga taaatgcata caaaagagac agaggaaaaa
     6121 atcttcaaag cagcatttgt gccttggata ttttcccatc tgtctctttt tgttattctt
     6181 tctgcttttt caacggattt tcttcataaa gagaagagat tgttgtgtac cattcaaaaa
     6241 catgagtaat cactacttta gctgctgcat aaacaggaat ccccaagatc acaccgatga
     6301 ttccgaacaa tttcccagaa gtcagcagaa ctaataaaat cgttactgga tggattgcta
     6361 actgattccc tagtactaat ggagagatca agcgtccttc tattgtctgc tctactgcaa
     6421 aaacgatcag tactttgatg atcatgaatg gacctgctac aatggctaaa aagatagccg
     6481 gaatcatcgc taaaaatgat cctaagtaag ggatcaggtt taaaaaaccc gcaataatcc
     6541 ccaacgtgac agcatactct aaaccaatga ccgcaaaacc aatcataaac atgattgcca
     6601 cagcaaaagc cacagttagt tgtcctctta tataagaaga aacttgacta ttcatttcac
     6661 tcagcacatt caacgtcggt ttttgcatgc gtgtcggtaa aaatttgaca aaataaggtg
     6721 ctaattgttt accgtctttc aatagataaa ataagataaa cggcatcgtc agcagagcca
     6781 ctaaaatagt cgctaccgcg ccgacaaaac ttccaagact ctgaactgta ctcttagata
     6841 tatcttggat catatcgcct gccgaattca tgaacttatc acctgctgtt tccaactgtt
     6901 cgcgaaattg gttgaacagc gggtcccgca aaatctcggt taatttatta tcaatcgtat
     6961 ctacatattg agggaagtta ctgatgaagc tcactgtttg ttcctgaatc ttagggatga
     7021 tcactacgct cccccatgcg attagtgcaa caaccaaaac aaacagccca atgatactgt
     7081 aaagccttgg tacttttcgc ttttcaaagt aatcaactac cggattcatc aagtaataca
     7141 agattcctgc taaaatgata ggcagaccta cgattgctaa aaattgccag attggtgcaa
     7201 acaaatatga aacttttgta aataaaaaga cgatcagcaa tattaacaat acaacaagta
     7261 aggctgtgac tacttgatta tttaaaaacc agcgccaaaa ccacgatacc ggtctgttct
     7321 tcttattttt tccttccata attcctcctg ttagttataa aaaatttggc ttcccaaacc
     7381 aatagcaggc agtctatgcg gatgaagata gattccattt gcaatttgtc cttcttctgg
     7441 acaagcatca aaaatcaatg atacgtgagc tactgtcttt aaattcccat tggccatcgg
     7501 accgacataa tcgatcgtat atacctgatc atcaaaagca attgttccgc ctgctttcaa
     7561 agaaaaaggt tcgtcttttg atacttcttg aatcaccgaa tattttcgta aggcagctgt
     7621 tgctgtttta tcgaacaaaa tcagcatggg ttcttccttg tttattgctt ctgtcccaat
     7681 ttctgtaatc tttgcctgaa tcattgcact ctctccttta attgctttca aaaacagtat
     7741 aacacattct cattctttag ccgtgtggaa taattatgtg atagaataaa aacgaaaact
     7801 atttcattac aattaggagg aatcagctca tatgatcgaa aagataattt taggaaccta
     7861 tacgcgtcgc gaaagtaaag gaatctatac gatcgacctt gatacagaaa aagaagaatt
     7921 agccaacctt tcattagctg ttgaagaaaa tagccctacc tatgtagcaa aaagtaaagc
     7981 tggcaatctt tacacagtta ctagtgtgga tggattaggt ggtgccggtg cgtacgataa
     8041 ggattttcat tttttgaatg cggtaacaga gtctggcgct cctttgtgtt atgtagcggt
     8101 tgatgaagca cgtcaattac tatacggggc gaactatcat aaaggtgaag taaatgtcta
     8161 ccatatatta ggagatggcg aactgaaagc agcagattca atattccatc aagaagcgac
     8221 aggtcctcat aaaaaccaag atcatgcgca tgtccactat acggatctga ctcctgacca
     8281 acgattagtc gtatgtgatc ttggtacaga tcgtgtgtat acttatgatg tgtccgaaaa
     8341 cggcaaattg aatcttgtaa ctaccttcac tgcagagcca ggaacaggcc ctcgccatct
     8401 tgtctttcat ccaaatggac aattcgctta tttatttggt gaattagaca gcacagtccg
     8461 cgtgctttct tataataaag aaacaggtgc ttttgaagaa ttacagaaaa tatcgacctt
     8521 gccagaagaa ttcgacggtg aaaatggagg ggcagctatc cgcatttcaa atgatggcaa
     8581 gttcctttat gcttctaacc gaggacataa ctcgattgct gtattcgcag tttttgaaga
     8641 tggttcccac attcaaaaca agcaaatcat ctcaacagaa ggggattttc cccgcgattt
     8701 cgcactagat ccaagcaata attttgtcgt gtgtgccaat cagaacaccg ataatctgac
     8761 attatatcgc cgtcatcctg aaacaggatt actggaatta ctgaaaaaag atgtctatgt
     8821 accagaatgt gtctgtgttt attttgatcg ttagtaagaa aaggagaatg aaatgaatca
     8881 gcaagacgta ttagacagac tacgggaaga actaaatatt cctttctttg atgggaaaat
     8941 cgaagataaa gagtatagcg aagaggaata tcagaaactc aaagccgatt tacagaacta
     9001 ctttgaacaa tatgtacaga atgtagaaaa ctaatcagct ttccatacta tctgaaaaga
     9061 acatcaggac aagacttttc cgtctatcct gatgttcttt tctacaaata aaaaaccgcc
     9121 gagatggtta gactcgacgg aaaaggagta tttactcgat tttgagtaag tacaaggttg
     9181 ttgttggtta taggtatgtc attatcataa aggattttga tcactctatc ataaaggtaa
     9241 tattacattt atattagata actctagttt ttattgttat caaagatttc ttttattttt
     9301 tcaacaacaa attagtcatt ttttggatgg cagctgtcaa ttggtctgtc gcttggtctt
     9361 gttttgcttc agatgtattg aatagatctt catctacccc tgattcaaga tcgatacttt
     9421 ggatcatttc ttcacagcct gctacatatg aaagataagc tttttcaaaa tttttatgaa
     9481 tgcccattgc ttttgctggt ggacgcaaag tactgacttt cataagcatt gcccgatatt
     9541 tttttgttcc ttcttcaaat aacaggatga tctcttcaag agtcgatttg tccagttcac
     9601 ttgtttttcc ttcatcaatt gcgtttctga tcacttcata tttcggatgc attccttctc
     9661 cgattgcttc tgtttccttg acgatttgat cgatggactg cacataatag ccaatattcg
     9721 gtaacatgtt cgttttccta ctttctccat tgttgttatt atttattcta cttgttttta
     9781 tcctcaaatg acaagtactt gaaaaaattt aatggtttct atacagctcg ccattcttat
     9841 tcgtgctcaa agaaaccatc tgctaaacta tattgtttcc ctgcgacaat gtcatattgg
     9901 atcaattgat attcgtcgta aatctcttgt tgcgagcgat tgaattgcgc ttctttatac
     9961 cactgtccgt tttgatagta cagttctcgt tcaaacgccg gaagttcctg ttctaatgat
    10021 aaaagcagct gataaaagcc atttgttgtc tgattcgtct gttccattag attcgctgca
    10081 aaataaaacg gactggtgat tgcatcgtga gtcgtttgct tttctaattc tcctttactg
    10141 tcgaacataa gaaattgtgt ttcatgcaat gtttgtttgt cattttttgc ttggatcgta
    10201 tctgaataga tccctggaag atgatccccc caaaaaacaa ctaaagtcct tctagaaaga
    10261 ttcttcaatt cttccgtgaa ctgcttcagt gccgtgctgc tataggaaat atcttgcaga
    10321 taattctcta agtcaagtgt cccacttcca tctgaagttt ttgcaggata gtctagatta
    10381 tcatactttc catcgtaggg catatgtgtc tgcatcgtga ctaaatgaat gaactgaggt
    10441 gttttttctt ccttgagaag tgtcattact tctttgtaag ctgactcatc agaaatatat
    10501 ggattattct ctatcgtatc cgtataggtc atcgtacgct cgctgatgaa ctggtcaaat
    10561 cctaatgtct gataaacatc ctctctttta tacatcgaag tattgtatgg atggatcgct
    10621 gtcgtctggt atgattgtgc gtttaacgct gaaacgatag aaggaagctg atccattttt
    10681 ggtacaagca tcgtgtaagg ggtggtgagt tgcgcattaa ataacgccat agaaaaacca
    10741 gtcaatgctt caaattcgat atttgctgtt ccaccgccat agttttgaga aagcatattg
    10801 ccactatatg tttgatcagc tacttcataa taatctgcca atggctcacc agatacttct
    10861 acgccgttta atcgactagg atctgagaaa ctttcactca tcacgaacac gatgttcggc
    10921 gactcttcct ctacagtttt atttttttca tcggctagct tctggtattt ttcagtgatt
    10981 tctttgatct tttctttcga atatccttca ggttcatcca ttggttctac cttcagattg
    11041 tacaaaaacc caccgataaa accagtgtta tagtagttca ttttctggct gtaaggtatc
    11101 catagtgccg ttttattata tgcttttcgc aacaaattat cgggattgtt gaaattactg
    11161 atatacccta gcagtccaat tgccacaagc agtgtcgtta atcggatcaa ttggaatcgc
    11221 cttccttttt tcaggcttcg aaacatgtac cagcaaaaca atcctatcac ggttcctgcg
    11281 gcagctaaga tcactgtaaa cagcatagtg ccggtcatat ctttcagcaa tccgatttca
    11341 gtgatcatct tcaaatcgtc tggataaatc ggttcttgcc gataactcat tttcatataa
    11401 tttgcaaaac ctaatacacc gatggctacg acatacgtaa gggaacctaa agggacagat
    11461 cctgctagtg caatcagaaa aataagaata atcagcaaga cgagacaggc taagaagaat
    11521 ttttccgtat gccaagaaaa agcaaatttc aacgcaaggt ctacggatag atcattttga
    11581 caccactgca aaaataaatt actaaaaaca acaagaaaaa agatactgat gattgctgct
    11641 gttcctttta aaatcttttt ctgtttggtc gaaagtctgc ttatatatct tttcaaatgc
    11701 ttgctccttc atttctatca ataaaagaat gttgcttctc ttattttagt tgtacgaaat
    11761 tcgaaagtaa agaacggaaa gaagaattaa tgaaatccaa atattctctt tctttatggc
    11821 ctttctggtt tctttaataa gcggaccttt cgtactccag aaattttttc tagctgttgt
    11881 ttcacttgat tgagctgttc aaaatcagta ctgtctatgt caatcaatat gtagacatgc
    11941 tcattcttat ggttacgcga tatatctgcg atcgctagct cgttctcatt cagcagagaa
    12001 aatatctctg ctaatatatt ggttttcttt tgataaaata tagtgaatcg aaacggcgct
    12061 tggaaaaaca ttcgtgcgga tggataattg accgcttctc taaccgtacc gaacaaaaga
    12121 taatctttta aggcttcaac tgccaaacgt ccgctgtctg ccaaagcttc ctgagtcgat
    12181 ccccctagat gaggcaacat aagtatacgg tcattttcta agaactcttc ttctggaaaa
    12241 tcagtcaagt atttagccag atgttgtttt tccaatgctt gcttgcataa cagcatattt
    12301 atcaacaatt tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat
    12361 attcgcctga tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta
    12421 atccgaggta gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc
    12481 gcggatagaa taacccaaga catccatccc taagctatag caacttagcg ctacttttgt
    12541 gcctatcgct ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata
    12601 agcacttctt ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat
    12661 acgacttgct tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt
    12721 tactgcatta gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt
    12781 ttcaacgttg atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt
    12841 aagccattca tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc
    12901 tattattttg aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat
    12961 caaatgaacg tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa
    13021 ttagacaact gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag
    13081 gctgagtgat aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca
    13141 aaatattgag ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag
    13201 actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact
    13261 aagtggcttt ttcacaacct ctttttatct aataaccact gtttccaact caactaattt
    13321 tgcccacgtt tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc
    13381 acctgcctga atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac
    13441 acgtgcaaca ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat
    13501 cagcagttta taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt
    13561 cccatcaaat acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac
    13621 gattcttggt ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat
    13681 tgcttcttgg ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc
    13741 tcgagccatg attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc
    13801 aaaaccgtat ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc
    13861 atacaggtct tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg
    13921 aataccgatt tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata
    13981 atcaccatag tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc
    14041 gtctgatacg cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca
    14101 accaaattga atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt
    14161 gtcaccaaaa cgagccactt ttattccaaa actttcatta taggctactg caacatccat
    14221 ccaatcagca atctcttttt ggatctcagg tcgtttccag tagccaacga cgattttatt
    14281 ttgtttattt aatcgagcat tgatgaaccc atattcgcgg tcaccatgag cggattgatt
    14341 taaattcatg aaatccatat cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc
    14401 taaatgaagc aatggttttt gtagaagctt tgtcccgcgg atccacattt ttgctggtga
    14461 gaaagtatgc atccaagtga tcacacctgc tacttcttct cgataattga cctctttcat
    14521 gatttttgtg atcgtatcag cagtaactgc taattcttga aggcggattg gataaggcaa
    14581 ttggccattt tcattcagtt catcgaccat ttcttgtgca tgtttcttta cttctcgcag
    14641 tgcttcttcc ccatataaat gctgtgatcc tacaacaaac caaaattctt tttctccaat
    14701 attcaacata attagccact ccttttattt gttttgtccg taatacgcat tttttccgtg
    14761 ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag
    14821 ctgtctggta aataaattca tcttgcatac ttcatctagt acgactgcgt tcattaccgc
    14881 actttgcgca tctttccccc aagtaaatgg accgtgtccg tgtaaaagta cccctggaat
    14941 tgctaaggga tcaattttgc gttctttgaa tgtttctatg ataacttttc ctgtttcata
    15001 ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta ccgatccata
    15061 aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt gtgcccagat
    15121 cgtcgcccat gtcgagtgag tatggacgat tcctccgatt tgaggaaatg aacgatataa
    15181 aacagcatga gtaggcgtat ccgaagaggg attgagttta ccttcaatga tatgattgtc
    15241 caagtccaca acgaccatgt cgtctgctgt catctcttca taactgactc cgctaggttt
    15301 gataacgaaa tatcgtgttt caggatcaaa agcgctcaca tttccccatg tatatttgac
    15361 gagtccgtgt ttaggcaagt caagatttgc ttggtatact tcttctttca gttgttccag
    15421 cattttctat cactcctttt tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc
    15481 cctcacggta acgtctcata aatatctgat agcctgctat ttcttctgct gaaggtgtga
    15541 tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa gaagttttct aaagacttcc
    15601 catgtcgatc catcatatag gctgctaata acgcgattcc ccacgctcca ccttctccgg
    15661 ctgtgtccat cactgtaact ggtgcctcca tagcagatgc tagaatccgc tgtccaactt
    15721 ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc aatttggaca ttctcagatt
    15781 tcaatatatc cattccgatt ctcattgcac caaaagcact ggctaaatgg atccgcatga
    15841 aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa taatggcctt ccttcattta
    15901 ctcctgtaat gttttcaccg gaaaaataac cataagaaag taatcccccg caatccgtat
    15961 cgccttctag cgctttgttg aataataccg tgaataattt atctctgctt atctccatac
    16021 ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc attgatatcc gaagagcagt
    16081 tattcgtatg aaccatgcct actagttctc ctgaaggtgt agtgaccatg tcgatttccg
    16141 gatggacatt ttctagttct ttttctaata cgatcatagc gaaggcggaa gtaccagccg
    16201 aaatgttccc tgtccctttg gctacactgt tcgtagcgac cataccggtt cctgcatccc
    16261 cttctggcgg acaaaccggg attcctggtc tcaaatttcc cgtagggtcg atcagataag
    16321 cccctgattc gcttaacttg ccagcttctt ctcctgctac tagaacttta ggcaaaatgt
    16381 gatccaagtc aatcgctacg tgcgcttttt cgattaactg accaaaaata tttttcattt
    16441 cttgatggta ggttttcgtt gaaggatcga taggaaacat gcctgatgca tctcctacgc
    16501 ctagtacttt ctcaccagta agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat
    16561 taatttcttt cacatgttct tctttattca aaatagattg atacaaatga gcgatactcc
    16621 atctctgcgg aatattgtat tgaaattttt ccgttaatat tcgttctgct tcttcagtga
    16681 ttgtatttcg ccaagtacga aatggtacta gtaactgttc cttgctatca aatgctagat
    16741 aaccgtgcat cattgcactg aacccaatcg acccgattgt cgtaagattc tcgccataga
    16801 tttcgaatac ttctgctgcc atttttcggt aactgacgcg cagtcctttc caaatatctt
    16861 ctaatgaata ggtccagata ccattttcta gtttgttttc ccactcataa ctaccagcgg
    16921 caataggagt aaaagatgaa tcaatcaata cagctttgat cctagttgat ccaaactcaa
    16981 tccctaaaga tgtacgacca tctatgacat ctaacatact ttgcgcattt acttccttca
    17041 taatgattta actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt
    17101 acaatgatta ttgtatactt ttgtacgtac atgtcaagca gttatttaca agaatacaat
    17161 aaaaaacact tgttttgtac gtacataagt tataaaatga aattgcttac aaaatctgat
    17221 caaccgcctt ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt
    17281 cctcatcgtt tatttatttt tttggcgcat ttggcggcat tctatttgga tatgatattg
    17341 gtgttatgac cggcgcctta ccttttttac agcatgattg gggtttggca ggtaaagctt
    17401 ctttgatagg atggatcacg tcttcggtca tgttaggtgc gatccttggc ggctctttat
    17461 ctggtcttct ttctgataag cttggacgac gaaaaatgat tcttctttct gcacttattt
    17521 ttatggctgg ttctgtcctt tctgcatctg cccctcacaa tggttcttat tttctgattg
    17581 cagcaaggat tcttctaggt ttagctgttg gcgctgcatc tgccctagtt cctgcttata
    17641 tgtctgaaat ggctcctgct agactaaggg gaagattgtc agggatcaat caagtgatga
    17701 ttgcttctgg catgctcctt tcctatgttg cagattattt gctaaaaggg ttgcctgaaa
    17761 cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg
    17821 gtgttttagc tctaccagag tcaccgcgtt ttcttatgca atctggccgc ttggaagagg
    17881 caaaaagagt attgaactat attcggacac caaaagaagc tgaacaggaa ttcgaacaaa
    17941 tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact ttgtttttag
    18001 aaaaatatcg ttccttagtc attgccggta ttggtgtagc agtattccag caatttcaag
    18061 gagctaatgc tattttttac tatattccgc tgatcgtaga aaaagcgact gggcatgcag
    18121 cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat tttagttgct ggttctttgc
    18181 tctttttggt tattgcagac aagttcaatc ggcgaacttt attgaaaata ggcggtagcg
    18241 tgatgggact ttctttcatc ctaccagctg ttttaggaac tgtactggat gctcatacaa
    18301 attcgttgtt gattctcttg tttttatgta tttatgtcgc attttattct tgcacatggg
    18361 ctccactgac atgggtaatc gtaggcgaaa tattcccact agctgtccgc gggcgagcat
    18421 ctggcctagc ctcttctttt aattggattg gttctttcct agttggcttg ctttttcctg
    18481 tgatgactgc ttctatgtct caagaaatcg ttttcggcat ttttgggatc atctgctttt
    18541 taggggtttt atttattcaa gagatcgtac cagaaactcg tgggaaatct ttagaggaaa
    18601 tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat ttctatccat cattcttaaa
    18661 gaggttgtga cagaagtggt cagcttcaag aaataagaag gaaccgctta gtttccagtc
    18721 acaaggaaca atttcaaaaa acagcttctc atgtttgatg aatattgtga cttgtgacca
    18781 gttggtagga gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga
    18841 tgacaaaagc tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa
    18901 aacccgtctc ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat
    18961 agagaatatt tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat
    19021 cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc
    19081 ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt
    19141 ctgtcaactt atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta
    19201 aagcttgttc ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg
    19261 tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga
    19321 taaaaccttt catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac
    19381 gctgatgtcc tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta
    19441 aacaaatata agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc
    19501 cttgttctct caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag
    19561 gctcaacgat caaaccatct accccataag caatcatttt cttcaaacac tctttctcca
    19621 attcgtgatc gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat
    19681 tttcgattcc tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac
    19741 cgatcgtttt tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg
    19801 tgcccgaccc tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc
    19861 gaatcgtatg tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg
    19921 tatttacttc gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat
    19981 attttgccat ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca
    20041 aggtttattc aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca
    20101 ccaaattctg gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta
    20161 ggattgtcta acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata
    20221 tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt
    20281 tttttagaag aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga
    20341 ttcttgtgct catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca
    20401 gaatacgtaa tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa
    20461 aaaccccgtt tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac
    20521 gtccaaggat tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct
    20581 tgttgggccc acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg
    20641 aaagtttccc ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct
    20701 atatgctgat tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta
    20761 tgattgcttg cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc
    20821 cagatcaatt cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca
    20881 tgttctaatt cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc
    20941 gatcctgtaa aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc
    21001 cgaacgatag gattattata aagtgtcttc atcataacaa atttcctcac attttcttta
    21061 ttctaattac attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta
    21121 ttgaaaaata gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca
    21181 acagatcgct actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt
    21241 caatcgttat cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc
    21301 cgttatctac gatcacactg tccacataag gaacattttt tatcttttca caatcataaa
    21361 cagggcaaat gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca
    21421 tcacataata agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta
    21481 ttgtgtttac taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag
    21541 ccagccctaa tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg
    21601 gactaccgac ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat
    21661 caaacgctag atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag
    21721 caacgatttc ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat
    21781 catcacgctc atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt
    21841 tatgcaaagc taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt
    21901 cagggtacga tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat
    21961 ccactaactt catgactttt gaggcttcat ttgctaacct accatattca taagcactct
    22021 tagcccccac ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa
    22081 aaggcttttt ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat
    22141 tacaatattc tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga
    22201 atatcggttt cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat
    22261 ttggttctat acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta
    22321 cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta
    22381 attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt
    22441 catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc
    22501 caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt
    22561 cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt
    22621 tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa
    22681 atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccat
//
LOCUS       DAYYAG010000035        22716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000035 DAYYAG010000000
VERSION     DAYYAG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.035"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..411
                     /locus_tag="ACHARQ_001188"
     CDS             1..411
                     /locus_tag="ACHARQ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8409621.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            404..1105
                     /locus_tag="ACHARQ_001189"
     CDS             404..1105
                     /locus_tag="ACHARQ_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8409622.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1105..2691
                     /locus_tag="ACHARQ_001190"
     CDS             1105..2691
                     /locus_tag="ACHARQ_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409623.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2710..2931
                     /locus_tag="ACHARQ_001191"
     CDS             2710..2931
                     /locus_tag="ACHARQ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8409624.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2928..3689
                     /locus_tag="ACHARQ_001192"
     CDS             2928..3689
                     /locus_tag="ACHARQ_001192"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8409625.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4069..4578
                     /locus_tag="ACHARQ_001193"
     CDS             4069..4578
                     /locus_tag="ACHARQ_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8409626.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4653..5192)
                     /locus_tag="ACHARQ_001194"
     CDS             complement(4653..5192)
                     /locus_tag="ACHARQ_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8409627.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5332..5943)
                     /gene="rpsD"
                     /locus_tag="ACHARQ_001195"
     CDS             complement(5332..5943)
                     /gene="rpsD"
                     /locus_tag="ACHARQ_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8409628.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6205..7056
                     /locus_tag="ACHARQ_001196"
     CDS             6205..7056
                     /locus_tag="ACHARQ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8409629.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7084..7719
                     /locus_tag="ACHARQ_001197"
                     /pseudo
     CDS             7084..7719
                     /locus_tag="ACHARQ_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            7739..8512
                     /locus_tag="ACHARQ_001198"
     CDS             7739..8512
                     /locus_tag="ACHARQ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8409630.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8920..9717
                     /locus_tag="ACHARQ_001199"
     CDS             8920..9717
                     /locus_tag="ACHARQ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8409631.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9695..10108
                     /locus_tag="ACHARQ_001200"
     CDS             9695..10108
                     /locus_tag="ACHARQ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8409632.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10092..12737
                     /gene="mutS"
                     /locus_tag="ACHARQ_001201"
     CDS             10092..12737
                     /gene="mutS"
                     /locus_tag="ACHARQ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8409633.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12755..14863
                     /gene="mutL"
                     /locus_tag="ACHARQ_001202"
     CDS             12755..14863
                     /gene="mutL"
                     /locus_tag="ACHARQ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8409634.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14885..15445
                     /locus_tag="ACHARQ_001203"
     CDS             14885..15445
                     /locus_tag="ACHARQ_001203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8409635.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15530..17518)
                     /gene="treP"
                     /locus_tag="ACHARQ_001204"
     CDS             complement(15530..17518)
                     /gene="treP"
                     /locus_tag="ACHARQ_001204"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8409636.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17741..18454
                     /gene="treR"
                     /locus_tag="ACHARQ_001205"
     CDS             17741..18454
                     /gene="treR"
                     /locus_tag="ACHARQ_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8409637.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18531..18977
                     /gene="msrB"
                     /locus_tag="ACHARQ_001206"
     CDS             18531..18977
                     /gene="msrB"
                     /locus_tag="ACHARQ_001206"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8409638.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            19147..19749
                     /gene="ruvA"
                     /locus_tag="ACHARQ_001207"
     CDS             19147..19749
                     /gene="ruvA"
                     /locus_tag="ACHARQ_001207"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8409639.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19762..20763
                     /gene="ruvB"
                     /locus_tag="ACHARQ_001208"
     CDS             19762..20763
                     /gene="ruvB"
                     /locus_tag="ACHARQ_001208"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8409640.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20792..21370
                     /locus_tag="ACHARQ_001209"
     CDS             20792..21370
                     /locus_tag="ACHARQ_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8409641.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21422..21904
                     /locus_tag="ACHARQ_001210"
     CDS             21422..21904
                     /locus_tag="ACHARQ_001210"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8409642.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22021..22395
                     /locus_tag="ACHARQ_001211"
     CDS             22021..22395
                     /locus_tag="ACHARQ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409643.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(22508..>22716)
                     /locus_tag="ACHARQ_001212"
     CDS             complement(22508..>22716)
                     /locus_tag="ACHARQ_001212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409644.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7388 a         4067 c         4913 g         6348 t
ORIGIN      
        1 atgaaatttt ttcaagagtt tttcattatt attttctttt ctgtattagg agaaggcata
       61 cggatttttg ctcatttacc tattcctgga agtatcttag gtattctttt tttattttta
      121 gcatttgaaa tgcatcttgt tgatcctgaa aaaatcggag caactggtga ttttttattg
      181 aataatctag ccattttatt tgttcctgct ggtgtaggtt tattggaata ttttgatgat
      241 attgcttcga tatggcctgt tttactggga gcagtcgtcg tgtgttccat cgtcacgatg
      301 gttgcagcgg gaaaaacggc agaaggtgta gaagctttgc tgggctatgt ccgtaagaaa
      361 aaagcagaaa gggtatctgt aaacgaaatg gaggagaatc aaattgactg agttttttgt
      421 gaatccatta tttggcttaa gtttgacttt aggagtgtac gtattatctg acaaattttt
      481 gcggaaatat aaactgcctg ttttgaatcc tctcgtatta tcgattgttg ttattatctt
      541 atttttgcat ttttctggga tcagctataa agactataat atcgggggca attttttgac
      601 tatgctgatt actcctgcga cagtggcact tgccatccct ctttataaaa acttccgttt
      661 actgaaagag aatttttatc ctgtaatggc tgcgatttta gcaggaatag tcgcaaattg
      721 cttggttagt gtagggttcg gttacttctt ctctcttcat aaagagatgg tgatctcatt
      781 actacctaaa tctgtaacga cggctatttc tgtcgattta tctcatacga tgggcggggt
      841 gaatgccgtg acacttgcta ttgttgtctc tacaggtatt tttggttcgc tgatcgccac
      901 tcatattttc cgcctattca agatcgaaag tccagttgct cgcggtgtgg cgttaggaag
      961 tacttctcat gctattggta cagcaaaagc aatcgagatt ggtgaaatag aaggaatcat
     1021 ttccggactt gcgatatgcg tcaacggaat attgaccgtt ttattgctac cgttattctt
     1081 tcagcctttt gctggcttgt tttgatgcga aaaccactat tttatgctac aataaccaaa
     1141 acgttcgagc ataaaaaagg agggaaagac gtgtatttgt cttccgagta tcttaaacaa
     1201 tttgataaag atctatatta taagattctc ctcctagaaa gttttgagga tcaagcttgg
     1261 cataccgctg cacaattagc gcaagttgtt cagctggatg cccgcagcgt gtcgaaatac
     1321 ctgaacgagc tgtctaaaaa ctatcagcag tttagcggta agacacaccc tttgtttaca
     1381 aaaaatcacc gttctggtta taacttttat gatacgctcg attcaattga acatgagcgt
     1441 tttttgatct atctcgtgca aagtacgctg aaattccagt tgctgcacga catttttttt
     1501 gaagaattcc atacaatgta ccaatttgcg caaaagcatt acattagtga atctactgct
     1561 catcgaaaaa tcaatgaatg gaaacagcaa ctgcaaactt acggaattcg gctgcaacga
     1621 ggtacatata tcgctcaagg cgaagaagaa atcattcgac tttacttgca tatgacattc
     1681 tggcagttgt ttcgagggaa aatctggcca tttgaaacga tttctcaaat ggacgtaaag
     1741 aatatggcag aacatatcat ggcttttttt aatgttcgtc tgaacgagat caaaaaaaga
     1801 cgattggaat atatgctagg tgcctttttt ttaagaaaat cacagaagca ttatgttgta
     1861 ttgaatgaga aaaaaagacg gctgatttct gacaatctgt tgttccagcg attttgccag
     1921 gtcatggagc cggtatttcc taattatttt caagtagaag acgagttagg cgcattattt
     1981 cttgtactga tgactcgtga agaatattat agtgatccta agatacggaa gaaaattttt
     2041 gattttcatc aacaagcaaa aacaccgccc tttacagccc tttcagaagc aaaagccgct
     2101 ctttctcttt atcaggaaga gcaggggctg ccagcagaaa atctaacctt cgaggcagaa
     2161 aactatttat tcagcagtca ttttttcgcc tatctttttc ctaatgcgaa agaaacgatt
     2221 gatggcaata gtagtgattt tattaatcat ttagttattg aaaacaagga attgaaacaa
     2281 tggctcgtac atttttttga aagtagacac aaacatccca atcatctggc atttaaaaat
     2341 catgcgtttt tgatggggcg gtatctgact gtgtttaaaa cattaggagc ctttactcca
     2401 cagcttccaa agatcacgat ccttttaatg acagatttcc cattatttga agagcaactg
     2461 ttagaggagg gattacggac atttttccgt aatgagtatc agctgatttt tctgccaaca
     2521 gattatcggg ggagagaagt tgatcttctg atttcgacaa gtaaagtcca ccgcaaacca
     2581 tgggcggatc ttgactattt tattgtgact gaagaactaa aattaattga ttacatccaa
     2641 ctatctcaaa aatttgaaat gattcaaaaa caaaaactat taaaacaata acaaagaagg
     2701 agaaaaaaga tgacatttga agaaatttta ccagaattga aaaaaggtgc aaagattatt
     2761 cgtaaaggat ggagtggatt tgaactttat gttgttctcg tatccgatga tgaatttgat
     2821 ggctgtccgg tgacgccgta cttattgatc aaaacaagtg acgaaggttt ttccagcttc
     2881 gctcctacag tttgtgatat tttagcagat gattgggcga tcgtagaatg aaaaaccagt
     2941 atccagatct agctaaacag gttgtattta tcacaggagc tgcttctgga attggtgctg
     3001 cgcaagcccg agcgtttctt gaacaagatg cttttgtatt tggattagat caaaagttcg
     3061 gtaaaatgga agaaatttct ttgcgatttc ctgaaacttt tgcctacgca ctaggtgatg
     3121 ttcgacaaat gaaagaactc caacaagcgg tatcgaaatg ccaaaatcat tttggtgaag
     3181 ttacgatttt actgaataca gcggggatat tggatgccta caaacccttg atggaaactg
     3241 acgaatcgct gtgggatctg atttatgaga cgaatgtgaa aagcatgtat cagctgacaa
     3301 aaatcatcct tcctgatatg atccgccaaa aaagcggaac gatcatcaat atggcttcga
     3361 ttgctggctt gattgctggt ggaggtggga tcgcttatac aagcgcgaaa catgcgattg
     3421 tcggatttac caaacaactc gctctggatt acgcaggaca gggtatccga gtaaaaggaa
     3481 ttgctccagg agccatccaa acaccaatga atgcggctga ttttgaaggg aaaggtgaga
     3541 tggcagagtg ggtggcaaat gaaacaccag tcaagcgatg ggcacagccg gaagaagtag
     3601 ctgagctgac gttatttctt gcctctcctc aagcaagcta catacaagga acgatcatcc
     3661 caatcgacgg cggatggttg ttaaaataac cttgtgtttt tttcaagctt ctgtatgata
     3721 aaactaaaga gagaaacaac ggttgatgat aaaaaaagtc gatgactgtt cccgacattc
     3781 gatgccgaac agccagttca aaaaattctt tttaataatc gttaatattc gaaactaagc
     3841 ggcttttcac gacctcttga tataaacgat attcaaaagc ttgcaactgc ggtaacaagt
     3901 ccagccaaat gaaaaatata aggagctatt tttatttgac cattcttatt acttgttgca
     3961 gggcagcttt ttcactacct tctgcgacac ttcagttgga attttcttcc catcctttgg
     4021 tcgcggtcat taccaagata aaattatctt gcaaaggaga aatagaacat ggaaaaaaat
     4081 aatacccgtt cagccaaacg ctggacaacg acagatgtga ctcggatggc gctagtcact
     4141 ggtttatatg tggcagtgac gctggttttg tctgtcttta gctttggagt gattcagatt
     4201 cgtctctcgg agatgttcaa ctatctgtcc ttgtataaca aaagatacat cgtcgcggta
     4261 acagcgggag tagctttggc taatattgct tctcctcttg gactgataga tgttgcagta
     4321 ggaagcgtca gcacgctgct tgtattgctt atcaactata aaatcactag tcgcatcaaa
     4381 aacatgaaaa tcaaaatggc cgtgacagca tgcgtatttg ctttttccat gtttacagta
     4441 gctggtcagc taacgatttt atatcaacta ccttttttct tgaattggtg ggtgattgct
     4501 ttaggagaat tggcatcaat gattgtcggt gggataatta tttattggat cggtaaaaaa
     4561 gtggatttgt caaaataaag atctgtttaa tttaaaatag ggcagcatgg aagagactga
     4621 atgttgagtc ttcttccatg ctgctttttt gattaatttg tatgaagaaa tcgatttagc
     4681 cgttttttca tcaagatacc gatataagct gctccagttg ccttgattgc ttccggcagt
     4741 aaaaacggca gtagtccact ggcaaatgca gttgtaaaag taagatcacc acttattttc
     4801 agccagatcg ttccgcagat caatgcagca agagagccca ttagattggc aaagattgca
     4861 tataagtaat gagcatccgt tctttcgatc atccaaccag ttatccacgt ttgtaagaga
     4921 aatccaatca aatatccgcc agttggccca aataaaacag ctatacctcc agtcatccct
     4981 gaaaatacag gaagaccaat caagccaagt aaaagataaa ctccaacaga tatcaatcct
     5041 agttttcttc cgagaaaagt tgctgttaat ccaatagcaa atgtttgccc tgtcaaagga
     5101 accaaaccaa aaggaatggt catctgagaa aggacagaaa taatcgcggc aaattccgca
     5161 gcaagtatca attctttcaa tgttcggttc ataacgccct cttttcgtta acttaatatt
     5221 tttatttggt taacgaaatt gtaaaacaaa gtgattactt tgtaaataga aaatataaaa
     5281 aaagaccagc tatccaatag atagcggtct ttttcaagaa agcttttgaa attataagct
     5341 ttggttgtag tattcaacga caagagcttc gtcgatttct ggatatagtt cgtcacgttc
     5401 tggtaaacga gtcaatgaac cttccaattt ttcagcgtca aagcttacga atgctgggcg
     5461 tccaacagtt gcttctacag cttcttttac tgttacgatg ttttgtgatt tttcacgaac
     5521 tgagatcact tgacccactt ctacgtggta tgaagggata tcaacgcgtt tgccatcaac
     5581 taaaatgtga ccatggttaa ctaattgacg tgcttgacga cgagtcgttg caagtcctaa
     5641 acggtaaaca acattgtcta aacgttgttc tagtaagatc atgaagttaa caccatgttt
     5701 accttctttg attttgctag cttttacgaa caagttacgg aattgacgtt cgttcattcc
     5761 gtacatgtga cgaagttttt gtttttcagt taattgcatg ccgtattctg attgtttgcc
     5821 acggctatta ggtccatgtt gacctggtgc gtaagggcga cgtgctaatt cttttcctgt
     5881 gcctgacaat gagatgccta agcgacgaga aattttccat gatggtcctg tataacgaga
     5941 cattaaaatt tcctccaatg aatataagtt tttggagtaa aataatccgt tgaaaaattc
     6001 ataatcatat agtttgttct tcaatcttca cctttgcagc cgcggttaca caatcgaacc
     6061 cgagacgggg caacaaacta gggatgagtt attatttttc tgctgcatta ttttacacaa
     6121 attacagtat atgatagatc atccacgaat gtcaagataa ttagtacata aaaacgcttt
     6181 acaaacctag gctgtcttta taaaatgaga gggtatgtgc aaatggaaaa aagcgaagga
     6241 agtttaagga gaaaatcaat gaatccggta tcaccattat ttgagaaaat cgatgcttca
     6301 attgaaaaga aggcgaatct ttttgaaaac agcttttcac gttacgctgt tcgagcggtt
     6361 ctcgcctgtt tgtttttgac tttaggaacg gctgtggcat ttgcgatcgc gatgaagggg
     6421 gaagatatcg cgcatggtct aggaaagatt ttatacgcat ttatgttcag ctggggtctg
     6481 gtcatgattc tatacatgaa cgccgaatta ggaacatcga atatgcttta tatgaccgtg
     6541 ggtgtttttc ggcagagact tgctttaagt aaggcagcca agattctatt tgcctgtatt
     6601 tttttcaata tgattggcgg tatattatgt ggatttctaa tttctatgac tacgccattt
     6661 caagatttaa cgttggacaa ctttatgtta gaatcaatag cagtaaaact agcaaaacct
     6721 tcagcaacga ttttggtgga agggatgttt gcgaatatcg ttgtcaatac cgctgtactg
     6781 atcagcatgc ggatgaaaga ggatgtcgga aaagtgatca cagtcgtatt catcattttc
     6841 atttttgctt ttttaggata tgaacacgtg atagcgaatt ttcctgcctt tacgcttact
     6901 tactttgctt ctcacggaca gatggatgga atgacagtca gcaatgtgtt acataatttg
     6961 ttttttgctt tggcaggaaa ttatattggt ggcggtttag taatgggact gggatatgct
     7021 tggctgaatc agtcaaaatc atcctatgtg gactaaaaag tcgttcatta aaggggaagt
     7081 gccatggaag ttttagtttt tccaaaaaat gaggaaaaag cgttacaaat ggaaaagtac
     7141 atgaaaaatc ttttttcctt tgctggagta cctaagccgg aacgcgctct tttacaacaa
     7201 ccgttactgg atgagagcaa aaatttatct atttctgaag ttttagcttt tgttcattat
     7261 tattatgaat aaatcgaaag agagtatcag tatgtagcga tcgatttagt ccagaaaaac
     7321 atcaaacggc ttacatttga agacttagtc gtattgttgc cactagttca gaaaaaagaa
     7381 tggtgggaca gcatcgactc ttggagaaaa gtatacagca catggtgtaa agcctaccca
     7441 gagcagcgtg aagaagtctt tcaattgttt gaaaaaaagg aagatttttg gctacgtcga
     7501 atagcaatca cgttgcaatt aggttataaa cagcaaacag acacaggact attgatccga
     7561 gcaatagacg aagatcgtca aacaaaggaa ttctttatcc aaaaagcaat tggttgggca
     7621 ttacgcgatt atagtaagac cgatgccgat tgggtaaaaa gacagctgac aacagatttg
     7681 agtaagttag ctgttcgaga ggcaagtaag tatttataag aaagcgagac gacaagagat
     7741 gagacgcaaa ttatttgctt ttgatattga tggaacgttg ttagattcaa acaaacaagc
     7801 tttagatagc acaagagaag cattggaaaa attacgcaaa gccggtcatc tggtcacgat
     7861 tgctacaggg cggagccgat ttcatgcgca agagatcatt cgcgatcttg attttacgaa
     7921 ctacatttta tgtaatggag cggccggttt tttagaccat gaacaagtat ataagaattt
     7981 actagatcgc gaccagttgg atgcgttcgt tcaagaagcg catcatgagc agatcgatac
     8041 agcttttgtc agcttagaca atatcaagcg ttcttcttcc aatgatgtaa cggtgatgga
     8101 agaagcgatg cattctttcg gtgctgtctt gcctgaatta gatcagtttt ttgcagagga
     8161 acaagaaata taccaagcgt tagcattttt cgatcaaaca tatgatggga agttctcttc
     8221 atatgataaa attcgatttg tccgctggca tgagaacagt gtagatgtcg tacctaaaaa
     8281 tggttctaaa gcagcgacaa ttctgcactt agcagaacaa gtaggcatcc ctcatgaaga
     8341 catcatcagc tttggcgatg gacaaaacga ccgtgaaatg ttgcgcatgt caggaattgg
     8401 gatagctatg gggaatgctg tgccagaagt acaagcagaa gctaagatgg tcactgatac
     8461 aaacgatcag gatggaattt ggaaggcatt gaaagagtta agcgtgattt aaaagaggtt
     8521 gtgaaaaagc tgttctgcat caagtaataa gcccagccaa acaaaaatag ctcctcctat
     8581 ttttcgtttt tctgggctta ttaccgcaga tgcaagcttt cgaacaccgt ttaaggaaga
     8641 ggtcgtgaca gaagtggtca gtttcaagaa ataagaaggg atttacgaaa attgcttctc
     8701 aaatttttgt aaaattacgg cttatttctg aagaagtaga tgaaacaacg tttattcggg
     8761 aaatgggact gtgacaagat gtttgtcata gtcccatttt tttattttat ttttcaaaac
     8821 taagaaatac caggaaagag ttccttttgt ttgacctttt ctttcttatt gaaaacaact
     8881 ataatataga gtgatgttaa aagagaagag gaatttagtg tgcgggtatt gtttattggt
     8941 gatgtagtag gttcaatggg acgagagatg attactgaat atcttcctag attgaagaag
     9001 aagtaccgtc cgcaagtaac gattgtaaat ggtgagaatg ccgcttctgg acgtggaatc
     9061 acagaaaaaa tctataaaaa atttttgcaa gacggcgtag atgtcgtgac gatggggaat
     9121 catacgtggg acaaccgcgg aatctttgag tttgtaaatg aagctaaaaa gatggttcgt
     9181 ccggcaaatt ttccggaagg gacgcctgga caagggatgg tctttgtcaa agtgaaccaa
     9241 gtcgaactgg cagtgatcaa catgcaggct cgttctttta tggtggactt ggatgatcct
     9301 tttcgtaaaa tgaatgagct ggtagaagaa gccagaaaac gtacgccgat catttttgta
     9361 gattttcatg gagaaacaac aagtgaaaaa caagcaatgg gctggttttt agatgggaaa
     9421 gtttcagcag tggtcggtac acatacacac gttcagacaa atgatgcgcg aattttgcca
     9481 agaggaacag cgtatctgac agacgttggg atgacaggtc cctatgatgg tattttgggt
     9541 atgcgcagag aacctgtaat cgaaaagttt ctgactgcat taccaaaacg ttttgaagtt
     9601 gtggaacaag gccgatcgat tttaagcggc tgtatcttgg aattggatga tacgacaggt
     9661 catgcaaaag aaatccagct gattcagatc aacgatgatc gaccgttcat ggagtagaaa
     9721 gagaggcagt tttttgcagc aggagccgga aattcaaaaa gaagtaagga aactgacaaa
     9781 acttttaaga gagaatgaaa cgatcattcg ctacaaagaa cttgaagaaa agatccagca
     9841 aaaccagtat ttagctgagt tgagagaaaa aatcaaacaa gcgcaaaaag atgctgttca
     9901 ttttgcccac tatgacaaac cagcagcaga aaaagaagca atcaaacaag cggatcaatt
     9961 catgcaagaa tttgatcagc atccgcttgt tgtagcttat agaaaacagc tgttggaagc
    10021 agatgatctg cttcatcatt taacaactat gatacaagaa gaaatcaatg gacaaataga
    10081 ggaggaaaaa catgcctcaa aaaactaaaa atactcctat gatggaacaa tatctaagca
    10141 tcaaggccca ataccaagat gcttttttgt tctatcgttt aggagacttt tatgaactat
    10201 tttacgaaga tgcgatcaaa gcgtcacagc ttttggaatt aacgttgacg agcagaaatc
    10261 gaaatgcaga agaacctatt cctatgtgcg gtgtacctca ccatgcagca caaggatata
    10321 tcgatgcctt gattgaaaaa ggctataaag tagcgatttg cgagcaggta gaagatccaa
    10381 agacgactaa agggatggtc aagcgcgaag tgatccagct agtcactcct ggtacagtca
    10441 tggacagcaa aggattgtcg gcaaaagaca acaatttttt aacagctgta gttagtcaag
    10501 gaaatgaatt tggctttgct tatgccgatt taagtacggg agaattgaag acggctcggt
    10561 tgcatgatga agaagctgtt ttaaatgaag cctctgcatt gcagacaaaa gaactcgtat
    10621 taggcagtga gatcactgat acccttcgtg aacagctaag cgggcgttta gggctagtct
    10681 tttctcaaca aaatgagatg gaagaaaacg cggaatttag ctttttgaca agcgaactag
    10741 tcgatccgct ggaaaaagaa gcaaccggca aactattgac ttatttatcg gtgacacaaa
    10801 agcggagttt agctcatatc caaaaagcag tagaatacca accagatcat tttttgaaaa
    10861 tggattatta ttcaaaattc aacctagaat taagccaatc tattcggact gggaaaaagc
    10921 acggcactct tctatggttg ttagatgaaa caaaaacagc aatgggtggc cgtttactaa
    10981 aacaatggct agaccgtccg cttatccagg caaaacaaat taaagcaaga caagaaatgg
    11041 tcgcttcttt gctagacagc tattttgaac gtatcgattt gcagtcagca ttaacgaaag
    11101 tctatgatct ggaacgccta gcaggtcgag tagctttcgg taatgtcaat gggcgagact
    11161 tgatccagct gaaaacttct ttagaacaag tgccaatgat cagagggttg attgaaggaa
    11221 tcgatcaagg tcaatggcaa agtctgcttt cagaaatgca gccgatggac catctcgtcc
    11281 aattgatcga ccaagctatc caagatgatc cgccgttaca aataacggaa ggaaacatca
    11341 tcaaagatgg attcaatgag cagctcgata tgtaccgttc tgctatgaga aacggaaaga
    11401 aatggttggc agaacttgaa gcaaaagagc gtcaagaaac aggaatcaag aacttaaaaa
    11461 tcggatttaa ccgcgttttc ggttattata tagaaattac aaaagcaaat cttggaaatg
    11521 cagaactgga gaagtatgaa cgaaaacaaa ctttagcaaa tgcagagcgt ttcatcacac
    11581 cagaactaaa agaattggag acacaaatcc tcgaagcaga agaaaaatct gttgatttag
    11641 aatatcagtt gtttttagct gtgagagaag aagtcaaaaa ggctatccag ccgctacaag
    11701 tacttgcaaa agcaatcagt gcagtagatg tgctacaaag ttttgcaacg atcagcgaac
    11761 gctatcagta tgttcggcca gaacttgtca gtgacaagca tcagcttctg atcaaagacg
    11821 gacgacatcc tgtagtagaa aaagtattag gacatcagga atatatccct aacagcgtag
    11881 aaatggcaga agacgaaatg atcttgctga ttacaggacc gaatatgtct ggtaaaagta
    11941 cgtatatgcg acagttggca ttaactgttc tgatggcgca aatgggctgt ttcgttccgg
    12001 caaaacaagc agttttacct atttttgatc ggatttttac ccgaattggt gcaagtgacg
    12061 acttaattgc tggacaaagt acttttatgg tagagatgat ggaagccaat caagccctgc
    12121 gttatgctac gccaaacagt ttgattttgt ttgacgaact agggcgagga acagcaacct
    12181 atgacggaat ggcgttggcc caagcaatca tcgaatatat ccatcgtcat gtacaagcaa
    12241 aaacactctt ttctactcat tatcatgaac tgacggtact tgagaaagaa cttccgcagc
    12301 ttaaaaatgt ccatgttgga gccgttgaaa aagacggaga agttgtcttc ttgcataaaa
    12361 tgatggatgg acctgcggat aaaagctacg gcatacatgt ggcaaagatc gcaggattgc
    12421 cttccaatct actagaacga gcagcagata tcctgcatac gctagaaaat ggcgataacg
    12481 gacaccatac tgttccagct gaagtagaag agaaaactaa aacagccaaa caggagcaaa
    12541 taaaaccacc tgcagaaaaa gtaaatcaag accatataca agaccgtgta aaagaagaaa
    12601 accaacagct ttctttattc agtgaattat ccgaaaatga aacagaagtc attgaatcac
    12661 tgaagcaaat aaatttacta gaaatgacgc caatggatgc actaaacaca ttatatgaat
    12721 tacaaaaaca actgtaggaa gaaggcggat atcgatggcc aaaatccaag aattatctga
    12781 acgtttagct aatcagatcg ctgcgggaga agtagtggag cgaccagctt ccgttgtgaa
    12841 agaattagtg gaaaatgcaa ttgacgccgg cagtactcag attgatatct tagttgaaga
    12901 agcgggactg cggacgatcc aagtgattga taacggagaa ggcattttgg cagacgacgt
    12961 agagaatgcc ttcaagcggc atgcgacaag taagatccat aatcgcgacg atctttttcg
    13021 catacgaacg ctgggctttc gaggggaagc tttgccaagt attgcctctg tttctgaaat
    13081 gactctggaa acggcgaatc aggaagaaaa acaaggcacc tatctgtcgc tgaaaggcgg
    13141 agaagttatt gagcatcgac cagcaccact gcgtcaagga acgaagatta ccgtcagcaa
    13201 tctgtttttc aatacgccag cccgcttgaa atacgtgaaa acactgcaca cagaattagc
    13261 gaacatcggg gacatcgtca atcgactggc attgagtcat ccatcgattg cttttcgttt
    13321 agtacatgat ggaaacaaaa tgctggcaac tgctggaaat ggcgatctga aacaaacaat
    13381 tgcgggaatc tacggattat ctaccgcaaa aaaaatgctg aaaatcgaag gaaaagatct
    13441 agattttgag ttatttggct atgtttcatt gcctgaggtg actcgtgcca gtaggaatta
    13501 cttatcgacg atcatcaatg gacgttttat caagaacttt tccttgaata aagcaatcgt
    13561 tgcaggttat gggtcaaaat taatggtcgg tcgtttccct attgctgtat tggaaatcaa
    13621 aatggacccg cttttagtag atgtaaatgt tcatccgacg aagcaggaag tccggctgtc
    13681 gaaggaaaaa gaactaacag agctaattag taacgccatt caaaaagcat tgtctcaaga
    13741 aaatctgatt ccaagtgcag cggataatct acggtttaag aaaaaagtac ctgatcagcc
    13801 taaaacagaa caaatcgaaa tcgatttgtc ctacgctttt gaagaagaaa aacctaaaaa
    13861 gccaagtagt ttgaattttg atcgacagac aggtaccttt ttcgtagaag aactaaagaa
    13921 cacatttgtt gacagaattg ttgataagtc tgtggaaaat gttgaaaact attctggttt
    13981 ttctgaagaa aacaccactt atccagaaaa aacagcgaaa acggtggata agtttggtga
    14041 aaatgttgat aactttgtag aaaaaacagt ggaaaaatct gaaaagaaag aatttcccat
    14101 tgtggataac atggagaaaa caaatgaaga acaagcagat aaatccgtag aagaggatga
    14161 aaagatttac gaggaagaat tgacactcca tcctgaattt gatttcactc aaggaaacga
    14221 caaaacatta gaacaagcca tcaagaaatt agaaacggaa aaaccagtac aacgttttcc
    14281 tcagttggaa tattttggac agatgcacgg aacgtatctt tttgcccaaa gtagcgatgg
    14341 gttgtacatc attgatcagc atgctgccca agagcgcatt aagtatgaat atttccggga
    14401 aaaaattggg gaagtatctg atgatctgca agaattgctt gttccatttg tgttagacta
    14461 tccaaacagt gatgctttga aattaaaaga gcaaaaacac ttgctagaag atgcagggat
    14521 ttatttagaa gaatttggtc aaaacagctt tattgtacgg gcccatccta cttggtatcc
    14581 caatggagaa gaagaatcga ttattcggga gatgatcgat atgctgctga caactggaag
    14641 tgtgagtgta aaaaaattcc gtgaagcaac agcaatcatg atgagttgca aacgatcgat
    14701 taaagccaac cattatttga atgaagcaca agcacgtgtg ctgctgaagg atctggcacg
    14761 atgcgaaaat cctttcaatt gtccccatgg ccgtccagta ttgattcact ttaccaattc
    14821 ggatatggaa aggatgttca aacgaatcca agacccgcat taagtcaaag agaagaggga
    14881 aaacatgaag attgtattag catcccaatc accaagaaga aaagagttat tggctcatat
    14941 cgtttcagaa ttcgagattg aacctgctga tatcgatgaa acgccatttc cagacgaaca
    15001 gcccgtcgat tatgttcgta gaatggctga agaaaaagca cgaactgtct gggaacaaga
    15061 tgaaaaagaa gaccatttag tgattgccag tgacacgact gttgttctca atcaagagat
    15121 catgggtaaa ccagaagatc tggctgaggc tgaattcatg ctgaaaaaac taagcgggga
    15181 aacacatact gtatatacag ctgttgtttt aaaaacaaag gaacgagaag aacggatact
    15241 agcagaagca catgtcactt tttatccttt gacagacgaa gagatcaaac gctacttgga
    15301 gacgggggat tatgcggata aggcaggggc atacggcatc caaaatgctg cgagtgtatt
    15361 cgtcaaagaa atttctggag attattacag tatcgtagga tttccgattg gtgctgtcaa
    15421 tcaggcactg aaaacattca tttaaacaaa aaagcggact atctgatttt cgattgaaaa
    15481 tccatgatag cccgtttttt ttattgaaaa gattacttat ctactctgac taagatttta
    15541 cttcagcttt accgactgct tcgcccgggc gaacaagtcc tgattggtca agagaaagtc
    15601 ctgcaacttt ttcattattg gtcaaagcaa caataatgtc agttttcttg ccagcttgtt
    15661 ctatttctgg caaatccatt tcagcaatca gggtctctgg tgtgacaagg tatccttctt
    15721 tcacgaatac attgaaagga acacccttta gttcaacagt atctaatccc atgtgaacaa
    15781 gtacttccag cccctcatta gaaagaatac caatggcatg ttttgtttca aagacactcg
    15841 tcactttacc gtttactggt gcatagactt tgccgtttga cggttcgaca gcataaccgt
    15901 cacccatcat tttttgtgag aaaactgggt catttacttc agttatttcc ttgatggtcc
    15961 ctgcagcgac tgcgaataat gtttcttttg ttgcaactgc cgtaccagtt gctgaaacag
    16021 gttcgaaaga ctgtggcgtt gctgttgcgc catttgctgt ttgaagttgt acatcagcag
    16081 cattttcagg aacagcagga tcaatcttat tcaaaattcc ctgacgtcta aagatgatcg
    16141 tcaagccaaa tggaatgatg acagcaatga tcattgcgat gatgaaaggt acccatgttt
    16201 ctgcttggat agctagtatt cctggcaaac cgcctactcc gatcgcgtta gctcgaacac
    16261 ccatcaagtt agcaaacatg ccagctaatc cagagccgac cattgcagcg acgaaaggat
    16321 agacgtattt caaattgatt ccgaacatag caggttcagt tacgcctaag taacaagaaa
    16381 tcattgctgg aatcgagatt tgttcttctt ttttattccc acgatgtaag aagatgatgg
    16441 ctaagactgc agagccttgg gcgatatttg ataaagcaat catcggccat aaattcgttg
    16501 atccgaaatc tgcaatcagc tgcatatcga tggcattggt catatggtgg agaccagtaa
    16561 tcactaaagg agcatacagg aatccgaata cggcactaaa caaccagctg atagaagacg
    16621 tcaatccagt atttacgata tttgagatcc agcttccgac tgtccagccg ataggaccta
    16681 aaataacgtg tgcagctaaa acagttggta acaatgaaaa caatggaaca aaaatcatcg
    16741 agatcgactg agggatatat ttgcggaaga agatttctag atacgctaac ataaaccctg
    16801 ccagcatggc tggaataact tgcgcttgat aaccaatcat ctggacttga gcaaaaccaa
    16861 aatcccaaaa tggaatatct gctgcggctg tggaagctac actataagca tttaataatt
    16921 gcggagaaac taacgtaatt cctaaaacga ttcctaatat ttgtgtagta cccatctttt
    16981 ttgcaatact ccaagtgata cctactggaa gaaaatggaa aattgcttca ccgggtaacc
    17041 acaaaaaggc attgacacca ttccaaaact tcgagtgttc aacgattgtt ccaccaagac
    17101 tttcaaactg gataccttca agtacatttc tgaatccaag gatcaatcca ccgacaatga
    17161 ttgcaggaat cagtggtgta aagatttctg ctaaaacggc aatagcacgt tgaacgggat
    17221 gaaggttttg ttttgcagct gcttttcctt gttctttgga aaccccttca actccagata
    17281 ctgcaacgaa gtcgttataa aaagtagaaa catcgtttcc tatgatgact tggaactgtc
    17341 ctgcattcgt gaacatccct tttacgctag gtatatcttc gatcgctttt tcgtccgctt
    17401 ttcctggatc attcaggaca aaacgcatac gagtcgcaca atgactaact gcagcaatat
    17461 tttcttttcc gccgatatca tgtaacagct tctcggcatc tgcttgatat tttgccatat
    17521 caataaaccc ctttctctta acttgtctat gcaagttgtt tcttcattta ctatactttt
    17581 tttcggagag aattgcaagc gttttattaa aaagtacgag agaaaatgat atcgttttaa
    17641 aaagcttaaa ataagctttt cttaaaaata ggaacattga aatacaaaaa gagctgtagt
    17701 atagtgaaga aatgatggaa gaacgagtga gggaaaaaac atgaataagt tcaatgaaat
    17761 ttttttagat ttggaacaga aaattttagc gaaagaatat cctccgcatt cgcttttacc
    17821 cagtgagaac caattgatca aaatttatgg tgtttcacga gaaacaatta gaaaagcgct
    17881 gaatcttttg actacggaag gttatattca aaaaaaacaa ggaaaaggtt cgatcgtact
    17941 ggatcggaat cgcttcgact ttcctatttc tggattgact agctataaag agctacagga
    18001 gacccaacgt attccaagtg aaacgatcat ccatacgtta gaagaaacag aagtgacaaa
    18061 agcattgaac gagatcactg gctgggagat cggtgcgcct gtttggcatt tgatacgcga
    18121 aagaaaaatc aatggcgaag tagttatatt agacaccgat tatctattaa aggaaatcgt
    18181 tcctatttta actccagcac aagcacaagg atcgatctat gaatattttg aaaatgagct
    18241 ttctttgacg attgcttacg cgcaaaagga gatcacagta gaagaagtaa cggaaatcat
    18301 gaaaacagcg atggatcttc acgatgacac ttatgcagta gtagtcagaa gcctcgttca
    18361 tctggaagat acccgctgtt tcgagtattc tgagtcggtt caccgattag ataaattccg
    18421 tttcgttgag tttgcccgac gccgaaaagt gtaaatagtt caaaagaaag caaattgttg
    18481 gcatctgtcc actttggcgc tatgatagga tcacaaaggg ggcattttta atggataaac
    18541 caataagacc agaaaaagaa gaactgaaag aaaagttatc acccatggct tatgcagtca
    18601 cacaagaaaa tgcaacagaa cgaccattta gcgggaaata tgatgatttt tacgaaaagg
    18661 ggatctatgt agatatcgtc agcggtgaac cgttattcag ctctgcagaa aaatatgatg
    18721 caggttgtgg ctggccatca ttcagcaaac caataacgaa aagacaagtg cgcgaaaaag
    18781 ccgatttttc tcatggcatg catcgaatag aagttcgaag caaagaagca gattctcatc
    18841 tcggacatgt gtttaccgat ggaccagtag atcaaggtgg tctacgttac tgcatcaatt
    18901 ctgcctcact gaaattcatt ccatatgaaa agatggatga attaggttat ggggtattca
    18961 aatcacttgt agaataatgg aaatacacaa agagcggtac aatacgggga tttactcgat
    19021 cgtccgctct ttttttattc aagcgattgc tatttgaaag aaacgctccc tagactcctt
    19081 ttttcacaag gtgaaatgtg ctacaataaa gggaacgtat gtgtgcaaaa gaaagaggac
    19141 tagaatatgt atgaatatct gacaggtgtg gttaccttta tcaaccccta ttacatagta
    19201 gtagaaacca atggtatcgg ctaccagatc gcactaggaa atccttaccg ctacagtagc
    19261 aaactaaatc aaaaaataaa aatttatgtc catcaggtaa tacgtgaaga tgcgcatctt
    19321 ctgtatggat ttgattcgct agaagaaaag caactgtttt tgcgattagt aagtgtttca
    19381 ggaatcggtc cgaaaagtgc ccttgcaatc atggcaagcg aagaccactc cggtttgatt
    19441 caagcagtag aatccagtga tgtcacttat ttgacgaaat ttccaggagt agggaaaaag
    19501 acagcacaac aaatgatctt ggatctaaaa ggaaaattcg gtgagctgag tatcgataca
    19561 ccttttagtc tatttgatga agcaaacaca aaagacgcta cagctctatc tgaagcaatg
    19621 gaagctctct cggctcttgg ttacagtgat cgagaaatca aacgtgtaga aaaacaatta
    19681 aaagaagtag ataatcagac aacggatgag tatctacgcc aagcactcaa gctaatgatg
    19741 aaaaaataag gaggagacca aatgacagat gaatacttgt tatcaccaga atctggcgaa
    19801 aatgaactga gtttggaaaa atctcttcga ccacagctgc tttctcaata tatcggacag
    19861 agaaaagtca aacaagaact tcagatttac atcgaagctg ccagaaatcg ggaagaacct
    19921 ttagatcatg ttctgttgta cgggcctcct ggattaggaa aaacgacgat ggccatggtc
    19981 attgctaatg agatgaccgt aaatattcgt acgacgagtg gccctgctat cgaacgtccg
    20041 ggtgacttag tagcaatctt gaatgaactg gaagcagggg atgtcctgtt tatcgatgag
    20101 atccatcgat tacctagagt ggtagaagag atgctttatt cagctatgga agatttctat
    20161 gtagatatca tggtaggaca agggccaacc gcacatccgg ttcatttccc tcttcctccc
    20221 tttactttga ttggcgcgac gacacgggca ggtatgcttt ctgcaccttt acgagatcga
    20281 tttggcatca tttctcatat ggaatattat gacgaacagg atctgcagga gatcgtttta
    20341 cgttcttcgg atatttttca aacagagatc attgaagaag gggcattgga aattgctcgc
    20401 cgatcacgag gaacgcctag aatcgctaat cgcttgctca aacgggttcg tgattttgcg
    20461 caagtccaag gagacgggaa aatcgatcgt cctatagcgg atcaggcact tactttgcta
    20521 caggtcgatc aggctggatt ggattatgtt gaccagaaat tgttgcgaac aatgatcgag
    20581 ttatatggcg gcggaccagt agggttaagt acgctttcag taaacatagg agaagagcgt
    20641 gagacggtag aagatatgta tgaaccgtat ctgatccaaa aaggattttt aaagcgtaca
    20701 cctagagggc gaattgctac tgcttatgcc tatgaacatt ttggttacga ctatcatata
    20761 tgatttttgc ttttagattg gaggaaaatg aatgagtgat acaacacgta cgaaacaatt
    20821 gatcaaaaac gtattgattg atttgtgcca aacgcaatct tttcgaaaaa tcagcgtcca
    20881 aaaaatcgca aacgaagcaa aaatcaatcg acaaacattt tattatcatt ttacagataa
    20941 atacgatttg cttcgttggg tctattgtga ggatgcattg cattatttag atccacaatg
    21001 tctttcactt gataattggg aagagcaagc attgaaaatg ctgaaagcaa ttgctgagaa
    21061 gaaagaattt tatgcaaata ctgtgctttc tgacccagaa attttacaaa aagagttttc
    21121 tcaactgatc caaaaatcat ttatcaagat ttttgaacaa atggatgaag aaagacaact
    21181 ttctgcaaga gacaaactat tttatgctcg ttttttttct tacggttgca gtggtgtatt
    21241 agtcaattgg atcacgaatg aatatgaaga aacaccgatg gaaatcgcta tgcagctttt
    21301 ccgtttagcc aaggatacgg aattatattc ttatcgtctt tatgcaaaag aagagttgga
    21361 agaacagtaa tagaaaaatt ttttgttgga aaagcaataa tcgctaaaag gagatgggga
    21421 aatgacaaaa attttatttg tttgtttggg aaatatttgc cgttcaccga tggcagaagg
    21481 cttgctgaga aaacgaatag cattagaagg aagggaagca gaattttttg tggattctgc
    21541 tgctacaagc acgtatgaag ttggaaagac accgcaccct ggaacgaaaa aaatattaaa
    21601 ccaagaaaaa gtagatatga cgaatatggt tgctcggcaa atcacacccc acgattttga
    21661 aacttttgat tggatcattg gcatggatca agaaaatgta gaagaactga aacgaagagc
    21721 accacgctca gcgcagggga aaatccattt gtttttaagt agcattcctg gaaaagaaaa
    21781 agaaaatgtt cccgacccat actataccaa taactttgat gagacatatc acttgatcac
    21841 agaaggcctg aatcattggt ggaatatttg gattgatgaa aataaaataa caaacaattt
    21901 gtaaagttaa cgctttcttt ttaaggcgat gttagagcat ttccttgttt ttttgtaaaa
    21961 tgttaggtaa aataaaatta attaagtaac actttcttag ctaaaggagg agtgcattcg
    22021 atgaacgaag agacagtatc gccaatgaag aagcttacga agttagttac tcatacttcg
    22081 tatcaacttt ttgattcttt tgtaaatgac actgaagaac atgctaagaa tgtacgattg
    22141 caaggaaagt ttattcttga gctgcaaaaa gctgctactg aacatagtct agttatctta
    22201 caagtaagag caaatcgttt gagtagcaag tatgaaacga tctcaggctg ggtggcgaca
    22261 aaaacggtcg aaaaagaccg agtgatgatc cgcctacaaa acgaagagca gcagatccgg
    22321 atggttcctg tagaacaaat tgaaaaaata acaatccttt caccagcagg ggaacaggaa
    22381 cggttatcaa aataatttat accagataga aaagcacgta tgagcttgta ccatcaatca
    22441 ttactaatca aaaataaccg aatgctttaa gctgatatca gtaaaacgga acgttttagg
    22501 atgagtatca tagcatgaca gcattcggtt gattagttga atcgattgga tggtacaagt
    22561 ttttttgata aaaaaaggtc ctgctagccg gggagctagc aggataagga gttaaaaatg
    22621 aaaaagtgtt gttagggttg tttgttggta tgcttatata atagtcatta gttgtgaata
    22681 ttttgtgacg aaagttttcg aaagttgtta agtttt
//
LOCUS       DAYYAG010000036        22639 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000036 DAYYAG010000000
VERSION     DAYYAG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.036"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACHARQ_001213"
     CDS             complement(228..1115)
                     /locus_tag="ACHARQ_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409645.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACHARQ_001214"
     CDS             complement(1112..1981)
                     /locus_tag="ACHARQ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409646.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACHARQ_001215"
     CDS             complement(2118..3404)
                     /locus_tag="ACHARQ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8409647.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACHARQ_001216"
     CDS             3677..4255
                     /locus_tag="ACHARQ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8409648.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACHARQ_001217"
     CDS             4255..5184
                     /locus_tag="ACHARQ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409649.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACHARQ_001218"
     CDS             5181..6917
                     /locus_tag="ACHARQ_001218"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8409650.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHARQ_001219"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHARQ_001219"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8409651.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACHARQ_001220"
     CDS             complement(7813..8322)
                     /locus_tag="ACHARQ_001220"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8409652.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHARQ_001221"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHARQ_001221"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8409653.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACHARQ_001222"
     CDS             complement(9623..10714)
                     /locus_tag="ACHARQ_001222"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8409654.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHARQ_001223"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHARQ_001223"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8409655.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHARQ_001224"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHARQ_001224"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8409656.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHARQ_001225"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHARQ_001225"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8409657.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14677..15651)
                     /locus_tag="ACHARQ_001226"
     CDS             complement(14677..15651)
                     /locus_tag="ACHARQ_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8409658.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15632..17515)
                     /locus_tag="ACHARQ_001227"
     CDS             complement(15632..17515)
                     /locus_tag="ACHARQ_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8409659.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17533..19758)
                     /locus_tag="ACHARQ_001228"
     CDS             complement(17533..19758)
                     /locus_tag="ACHARQ_001228"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8409660.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19873..20907
                     /locus_tag="ACHARQ_001229"
     CDS             19873..20907
                     /locus_tag="ACHARQ_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8409661.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20974..21987)
                     /locus_tag="ACHARQ_001230"
     CDS             complement(20974..21987)
                     /locus_tag="ACHARQ_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8409662.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22065..22253)
                     /locus_tag="ACHARQ_001231"
     CDS             complement(22065..22253)
                     /locus_tag="ACHARQ_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8409663.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6450 a         4863 c         4096 g         7230 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    14461 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    14521 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    14581 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    14641 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    14701 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    14761 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    14821 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    14881 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    14941 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    15001 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    15061 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    15121 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    15181 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    15241 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    15301 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    15361 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    15421 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    15481 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    15541 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    15601 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    15661 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    15721 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    15781 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    15841 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    15901 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    15961 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    16021 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    16081 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    16141 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    16201 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    16261 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    16321 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    16381 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    16441 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    16501 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    16561 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    16621 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    16681 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    16741 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    16801 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    16861 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    16921 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    16981 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    17041 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    17101 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    17161 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    17221 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    17281 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    17341 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    17401 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    17461 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    17521 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    17581 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    17641 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    17701 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    17761 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    17821 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    17881 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    17941 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    18001 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    18061 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    18121 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    18181 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    18241 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    18301 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    18361 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    18421 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    18481 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    18541 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    18601 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    18661 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    18721 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    18781 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    18841 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    18901 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    18961 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    19021 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    19081 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    19141 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    19201 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    19261 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    19321 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    19381 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    19441 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    19501 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    19561 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    19621 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    19681 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    19741 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    19801 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    19861 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    19921 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    19981 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    20041 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    20101 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    20161 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    20221 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    20281 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    20341 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    20401 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    20461 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    20521 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    20581 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    20641 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    20701 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    20761 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    20821 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    20881 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    20941 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    21001 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    21061 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    21121 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    21181 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    21241 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    21301 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    21361 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    21421 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    21481 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    21541 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    21601 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    21661 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    21721 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    21781 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    21841 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    21901 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    21961 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    22021 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    22081 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    22141 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    22201 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    22261 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    22321 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    22381 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    22441 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    22501 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    22561 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    22621 taatgtagac taactaaaa
//
LOCUS       DAYYAG010000037        22165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000037 DAYYAG010000000
VERSION     DAYYAG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.037"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACHARQ_001232"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACHARQ_001232"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8409664.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACHARQ_001233"
     CDS             2370..3848
                     /locus_tag="ACHARQ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8409665.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACHARQ_001234"
     CDS             3814..5145
                     /locus_tag="ACHARQ_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8409666.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACHARQ_001235"
     CDS             5259..7811
                     /locus_tag="ACHARQ_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8409667.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACHARQ_001236"
     CDS             complement(7818..9257)
                     /locus_tag="ACHARQ_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409668.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACHARQ_001237"
     CDS             9232..9414
                     /locus_tag="ACHARQ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409669.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACHARQ_001238"
     CDS             9603..9965
                     /locus_tag="ACHARQ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8409670.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACHARQ_001239"
     CDS             complement(10152..11009)
                     /locus_tag="ACHARQ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8409671.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACHARQ_001240"
     CDS             11129..12040
                     /locus_tag="ACHARQ_001240"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8409672.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13698
                     /gene="mdcA"
                     /locus_tag="ACHARQ_001241"
                     /pseudo
     CDS             12047..13698
                     /gene="mdcA"
                     /locus_tag="ACHARQ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13800..14099
                     /locus_tag="ACHARQ_001242"
     CDS             13800..14099
                     /locus_tag="ACHARQ_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8409673.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14089..15765
                     /gene="mdcD"
                     /locus_tag="ACHARQ_001243"
     CDS             14089..15765
                     /gene="mdcD"
                     /locus_tag="ACHARQ_001243"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8409674.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15769..16362
                     /locus_tag="ACHARQ_001244"
     CDS             15769..16362
                     /locus_tag="ACHARQ_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8409675.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16386..17462
                     /locus_tag="ACHARQ_001245"
     CDS             16386..17462
                     /locus_tag="ACHARQ_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8409676.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17487..18356
                     /gene="mdcB"
                     /locus_tag="ACHARQ_001246"
     CDS             17487..18356
                     /gene="mdcB"
                     /locus_tag="ACHARQ_001246"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8409677.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18467..19231
                     /locus_tag="ACHARQ_001247"
     CDS             18467..19231
                     /locus_tag="ACHARQ_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8409678.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19720..21813
                     /locus_tag="ACHARQ_001248"
     CDS             19720..21813
                     /locus_tag="ACHARQ_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8409679.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21838..22140
                     /locus_tag="ACHARQ_001249"
     CDS             21838..22140
                     /locus_tag="ACHARQ_001249"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8409680.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3787 c         4722 g         6477 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaatgca
    13381 ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg aattgcttat
    13441 ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat tggcggtgtt
    13501 acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc agccggcatc
    13561 gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc attattagct
    13621 gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc accaatgaaa
    13681 ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta cccagatgga
    13741 agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg gagagatcca
    13801 tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa atccatgtag
    13861 gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa acagaatcta
    13921 ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat gtattaacga
    13981 gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc ggtgctacac
    14041 caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat gaaaaatagt
    14101 tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg acgtgaattg
    14161 attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat cgtccctgaa
    14221 agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc agtagtaatc
    14281 gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc aaaaatcgtt
    14341 gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt tccagtcatt
    14401 gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt atctatttca
    14461 gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg tttgattcca
    14521 ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag ttatttaatc
    14581 gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca agaagcaggc
    14641 gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg atctcgtcaa
    14701 cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc aatcaaagct
    14761 gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag ctcagcattt
    14821 tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga cgattacaat
    14881 gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc agaaggaaat
    14941 gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg catgaaaaat
    15001 ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat agaagaagcc
    15061 atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg aaacggtgaa
    15121 gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta cgaagaagat
    15181 aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag ccaagcatac
    15241 ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc tgctgcttat
    15301 gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa tgctatttca
    15361 ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa caataaagaa
    15421 attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg ttcggttgct
    15481 gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa taattataca
    15541 aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc ttcagaacaa
    15601 aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg caatacaaat
    15661 gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg acgtgtcgaa
    15721 acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat gattcaagca
    15781 catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc tgaatgggtc
    15841 gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca cgcagaaata
    15901 ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga aatctcaaaa
    15961 catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc gtatagacaa
    16021 gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa catattatca
    16081 ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg gattccggca
    16141 gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat tgaattgcca
    16201 attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca agtagtgact
    16261 cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa aattctattg
    16321 aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa aagagagaga
    16381 aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag aaagtatcag
    16441 ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac agccacagcg
    16501 caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca gcaccaatgt
    16561 ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct tattgggaat
    16621 ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg acggcttatc
    16681 tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca agctttttac
    16741 tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg atgctgcaga
    16801 tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct aataaaaaat
    16861 atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat ctgttctatc
    16921 aaacacaagt attgactggg ccttatcatg acagctggta cttattagat cggtttgttg
    16981 ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc tatcaatatt
    17041 tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc ttttctgtcc
    17101 gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac ttgaatacga
    17161 tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct tccaaaatga
    17221 ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat gcttatcgta
    17281 cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa gatagttggc
    17341 tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca agttgtgcat
    17401 ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt ttcagcaagt
    17461 aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa gcagtgactc
    17521 agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta agtccaaaac
    17581 ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat gctttatttc
    17641 ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata gcatggcatc
    17701 atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa gcggaaaagg
    17761 caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg gcaatggggc
    17821 tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct ttatcgaaaa
    17881 tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt cataaaaaaa
    17941 ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat gaagaagcga
    18001 tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta gataatgatt
    18061 catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta gatgacacat
    18121 gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt gctcatgctg
    18181 ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt tgtcaggaaa
    18241 accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc atgctgaaga
    18301 tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat gactaaataa
    18361 gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct tttagcaagg
    18421 ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga aaaaaatcaa
    18481 ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga cccaaggatt
    18541 cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg gcagaggttc
    18601 atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg gcagagattc
    18661 ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg gatttcttgc
    18721 attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga ataaaagaca
    18781 aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta aagaaaagaa
    18841 agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt gggaagaaga
    18901 aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac atcagaaagt
    18961 tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa ctattcagga
    19021 agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg atttcagatg
    19081 ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta agacacaacg
    19141 acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa gatggcaagc
    19201 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg gagttgtgac
    19261 aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata gcctatcggc
    19321 aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt cttatcgctc
    19381 aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc ttgcatctgc
    19441 gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt tgttcttatt
    19501 acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca aaatttttgt
    19561 aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct tattttttgt
    19621 ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt tattatgtaa
    19681 gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag aacaattgaa
    19741 atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct agcaagtgag
    19801 ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat ctggttgagc
    19861 agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt gaagctggaa
    19921 aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg ctttttgaat
    19981 cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat cccgatccat
    20041 ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga agatatgaga
    20101 cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg aaagcaagga
    20161 ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt tatcaatcag
    20221 aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc ggctcaaaaa
    20281 atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat ctattccaat
    20341 aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt tacattgatc
    20401 tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa aaatacagaa
    20461 ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt tcaattggac
    20521 aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt caatgctaga
    20581 gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca attgatccaa
    20641 tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt attcgaaaat
    20701 ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca agtcatgaat
    20761 ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt cactcatttt
    20821 gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat agcttttctt
    20881 gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta tgtttataaa
    20941 tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga gaatctgaaa
    21001 gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat cgaattagta
    21061 gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg aaataagccg
    21121 cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga agactttttg
    21181 aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac ggagttattc
    21241 actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat catctatggc
    21301 aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga gatccagcct
    21361 atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc cactacttgg
    21421 gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt cgaagagcgc
    21481 tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt gattggcaaa
    21541 cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg agtcagcgta
    21601 gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt aaagattatt
    21661 ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga actaattgaa
    21721 ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat cgaagatttc
    21781 aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag aacaaagatg
    21841 aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt tgcagcacag
    21901 atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga ccattgtgat
    21961 atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa ttttcgttcg
    22021 cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa aaatattgtt
    22081 tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg agttatctaa
    22141 atacaaaaag aaaagagggt aaaaa
//
LOCUS       DAYYAG010000038        22151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000038 DAYYAG010000000
VERSION     DAYYAG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.038"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..541
                     /locus_tag="ACHARQ_001250"
     CDS             20..541
                     /locus_tag="ACHARQ_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8409681.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            638..859
                     /locus_tag="ACHARQ_001251"
     CDS             638..859
                     /locus_tag="ACHARQ_001251"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8409682.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1016..1180)
                     /locus_tag="ACHARQ_001252"
     CDS             complement(1016..1180)
                     /locus_tag="ACHARQ_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8409683.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1283..1804
                     /locus_tag="ACHARQ_001253"
     CDS             1283..1804
                     /locus_tag="ACHARQ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8409684.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1791..2231
                     /locus_tag="ACHARQ_001254"
                     /pseudo
     CDS             1791..2231
                     /locus_tag="ACHARQ_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2271..2429)
                     /locus_tag="ACHARQ_001255"
     CDS             complement(2271..2429)
                     /locus_tag="ACHARQ_001255"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8409685.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2522..3283
                     /locus_tag="ACHARQ_001256"
     CDS             2522..3283
                     /locus_tag="ACHARQ_001256"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8409686.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3470..4822
                     /locus_tag="ACHARQ_001257"
                     /pseudo
     CDS             3470..4822
                     /locus_tag="ACHARQ_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4838..6058
                     /locus_tag="ACHARQ_001258"
     CDS             4838..6058
                     /locus_tag="ACHARQ_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8409687.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6093..6770
                     /locus_tag="ACHARQ_001259"
     CDS             6093..6770
                     /locus_tag="ACHARQ_001259"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8409688.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6767..7963
                     /locus_tag="ACHARQ_001260"
     CDS             6767..7963
                     /locus_tag="ACHARQ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8409689.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7978..8301
                     /locus_tag="ACHARQ_001261"
     CDS             7978..8301
                     /locus_tag="ACHARQ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8409690.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8305..8640
                     /locus_tag="ACHARQ_001262"
     CDS             8305..8640
                     /locus_tag="ACHARQ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8409691.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8637..9032
                     /locus_tag="ACHARQ_001263"
     CDS             8637..9032
                     /locus_tag="ACHARQ_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8409692.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9029..9400
                     /locus_tag="ACHARQ_001264"
     CDS             9029..9400
                     /locus_tag="ACHARQ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409693.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9416..9976
                     /locus_tag="ACHARQ_001265"
     CDS             9416..9976
                     /locus_tag="ACHARQ_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8409694.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10103..10414
                     /locus_tag="ACHARQ_001266"
     CDS             10103..10414
                     /locus_tag="ACHARQ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409695.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10438..10629
                     /locus_tag="ACHARQ_001267"
     CDS             10438..10629
                     /locus_tag="ACHARQ_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409696.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10629..14474
                     /locus_tag="ACHARQ_001268"
     CDS             10629..14474
                     /locus_tag="ACHARQ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8409697.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14475..15347
                     /locus_tag="ACHARQ_001269"
     CDS             14475..15347
                     /locus_tag="ACHARQ_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8409698.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15344..16393
                     /locus_tag="ACHARQ_001270"
     CDS             15344..16393
                     /locus_tag="ACHARQ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409699.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16397..17221
                     /locus_tag="ACHARQ_001271"
     CDS             16397..17221
                     /locus_tag="ACHARQ_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409700.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17239..19371
                     /locus_tag="ACHARQ_001272"
     CDS             17239..19371
                     /locus_tag="ACHARQ_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8409701.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19388..19537
                     /locus_tag="ACHARQ_001273"
     CDS             19388..19537
                     /locus_tag="ACHARQ_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409702.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19534..19980
                     /locus_tag="ACHARQ_001274"
     CDS             19534..19980
                     /locus_tag="ACHARQ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409703.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19982..20119
                     /locus_tag="ACHARQ_001275"
     CDS             19982..20119
                     /locus_tag="ACHARQ_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8409704.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20156..20449
                     /locus_tag="ACHARQ_001276"
     CDS             20156..20449
                     /locus_tag="ACHARQ_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409705.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20472..20669
                     /locus_tag="ACHARQ_001277"
     CDS             20472..20669
                     /locus_tag="ACHARQ_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8409706.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20680..21699
                     /locus_tag="ACHARQ_001278"
     CDS             20680..21699
                     /locus_tag="ACHARQ_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8409707.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21956..>22151
                     /locus_tag="ACHARQ_001279"
     CDS             21956..>22151
                     /locus_tag="ACHARQ_001279"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8409708.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGK"
BASE COUNT         7708 a         3773 c         4912 g         5758 t
ORIGIN      
        1 ggattaagga ggaagagtaa tgcatatagg agataagata cataaattga gacggcttaa
       61 taacgaaaca atgaacgact tagttaaaca aaccggactg tcaaaatcat gggtatctga
      121 tattgagtct ggaaaaaagc aacgcgctga ctttaataag atatgtgcaa tagccaaaca
      181 ttataaagtt agcttagaat actttcagga tgattatttg gatagtgatg aacacgagga
      241 tattattcct ttgacagacg aaacaagagt taaatcagtg aaatcgcttg aacaaagcct
      301 aaagtatctg aataacaaac aagaacctaa ccttctagag atcaaactaa aagatactga
      361 ctcagtacca gaggtttatt acaaaggtca gaagttagag ggattgatag atgtaagcta
      421 tcactaccat acaacaactg atgtggtagc tggttcaaca aacgacatca atatggagta
      481 cctaggtgaa gtaactaagt atccagatat aaaaacaatc ggacacaaga gagatgtgta
      541 aatgaaagaa gctagaccta gagacgagat agacaaacta tacaagacca aacgatggcg
      601 agacctaagg caagtagtaa tagctaggga cttcggcatg tgccaagagt gcaagcgtcg
      661 agggcggaac acaaggggca cgatcatcca tcacatagtc gaggcgaggg aagacctgtc
      721 actgttctgg tccgtagata accttgaatg tatctgtgta gcttgtcaca acagagagca
      781 tccagagaga tcaggcggga agaagaaacc aaaacctaaa tcacatatcg ttaaaatgta
      841 ttcaactcct gaaagataag tttgcagcga aatgaaggta gcccccctac tctaaaagat
      901 taaagagtaa ggcttgagaa gaacggtgct gtccttcctt cgtaaaaaga ccgcttttca
      961 agttttttgg agaaaaagga aaaagccgac caatttaagc cggctttgga cgaagctatt
     1021 tcttagtcca tttgtttcct ttttgagaag taggaggtaa tcggtcgcct ggatcaattt
     1081 ttacttctcg tccgccttgg acatttccac cacgaggtcc cacttcttta taggttcctt
     1141 ttggtttatt gtcttcgccg ggtttataga gttctcccat aggaatccct ccctaaaaaa
     1201 atttcggcac agcactgccg ataacttaat tataaggatt gtgataacga tttcaatata
     1261 tcttttgaaa gaaggtgata ttatgccgca accagcgaag agtgcaaaat tacaattatt
     1321 aaacggaaac ccaaataaga agaataccga agaactccgc aagcgagcgg ccgcagaaga
     1381 caaattaaaa atggctactg acaaaatcaa accgccgtta tggctagatt cgctaggaaa
     1441 ggataccttt gagtttatcg ctgatgaatt gctgtctgtg gatttaatca gtaatccgga
     1501 cgtccataca atggctctct actccaattg gtattcgcaa tacgtttctt tagaaaaaca
     1561 gcttcgaaaa ctacaacgag agtacaagtt gaactatgcg cttgcgaaaa aggaggcaga
     1621 ggcgagaggt gagccgttta atgaacctaa tgaattaatt ggtaatccgc tctctcggca
     1681 gatggataca gcgtcgcgga atctccgttc ttttggcgct gatttaggac tatcaccagc
     1741 agccagagct aagttagcta ttaagatggc tgatgatggt ggtgatgacg atgacgactt
     1801 ctaatatttt ggatatgtcc tacacagaac gtgtggacta ttggcaaagc tatcttgagg
     1861 agcaagcttc ttggggtggc tttttaaaat gtccatatcc ggaattgtta actacttggt
     1921 atgcggaacg attaatcgat ggaagcatac cagccagcaa agaaaatatt caagctgcta
     1981 aacggcatat gcgtgatttg cagcgccaag gaacagatga ttttccttgg atctttgacg
     2041 aagaaaaagg tcaccggcct attagatata tcgaaaaaaa atgtaaacca actgaaggcg
     2101 actttggttc gtttgttttg caaccttggc agcatttcat aattggatcc atgtacggat
     2161 gggtacatcg tgatacagga gagcgtcgct tccgcgaggc tcttattttt gttggacgta
     2221 aaaacggtaa gttttgccgt ttaaaaatcg ggcaaaatcg gtgaaaacct ttatttttgg
     2281 ctttctttta gtaataattc gatagcttta tcaagcaatt tagacatagg aaccatagtt
     2341 tcttccgaca ttttttttaa tccttcgtat aaatctttat caattgcgtt agaaattcta
     2401 attctgtttt ttagtccgta tttgtccatg tgtgacactc ctttttattt tactataaca
     2461 tccgattcca ccgcttgcaa ctaccatcaa ttgatggtag tatataaaag aggtgagtag
     2521 attgaaaaaa tacattattt acaaacactt aaataaaata aatggcaaaa tttatatcgg
     2581 tgtcacaaat gatattggca gaaggtggag gagcggtggg attgaatata aaccaccaaa
     2641 gaacgaaacc caacaccaca gaagtttttg gaatgcaata caaaaatacg ggtgggaaaa
     2701 ttttgatcat ttgattattg aagaagactt aacgatgaaa gaagcttttg agaaagaaaa
     2761 gttttatatt gaactttatg actcaactaa taagaaaaaa ggttataaca tcgcaaaagg
     2821 cggaaatggc ggaataatat ataaagttca ccccaaagga atgctaggga aaaaacaatc
     2881 taaagaattt tcttcaaatc acagtaaatg ggcaaagaac cataagaata attgtatgac
     2941 aaacggtgat gttgtatggg gtgttactca cgaacaccca aaaggcatgt tgggaaagca
     3001 ccacagcaaa gaatcaatta tgaaaaagaa agcatatagt ggtgaaaatg ctgtaacctc
     3061 aaaaccaatt gtcgcaattg aacgggatgg aactaaaaga gaatttcaca gcgctaagct
     3121 ttgcatggct tactattcga ttagtacatg tgttttttac cgacttttaa aagatggcgc
     3181 cccttatgtg ataaatccta aagcgaacta cagaaataaa gaaaaaatat tagctatcga
     3241 aggaattatg ttcaaacata aagaagatac cgaggtaagt tagtagattg cgaaaggtta
     3301 ctaactaccg taacgcgtag gagttgaata aatataatgc tcccaagagt gtccgacagc
     3361 agataatttg ctgaaaatgt acgctaaact gggccagaac tgactgaccg atgaaaatga
     3421 gggtgacctc cagagtgcga gataaaaagc tcgcagataa taacaatcga aaacatcact
     3481 aatttcaggc ctttccacat acatggtcgc ttatgatgat gaacaaggcg ccaacgttta
     3541 cgtattggca aatgctcgtg atcaagcaag cttgttgttt gataaggccg cagaaatggt
     3601 caaacaatcg ccggcgctct ttaagaaatt tggtaagcct aaacgatcaa gtattaatta
     3661 tgctcccgcc ttttctaaaa tggaaccacg cgcctcagat agccggaaat tggatgggct
     3721 aaacactcac tttggtattt ttgacgagat ccacgagttt acgaattaca atctgatcaa
     3781 cgttatcaag aaatcaagag gaaccagaaa acagcctctg atagtttata tcacaactgc
     3841 tggatatgta ttagatggtc cgttgatgtc ttattttgag caaggtgtgg actgtttgga
     3901 acatttggaa gatgacatcg atgaacggac tttctattat ctggcaaaac ttgacagtgc
     3961 ggaagaggct gatgacccaa gattatggat caaagccaat ccgaatattt gtctaatgaa
     4021 ttttgttggc atgctagatg actatgttaa ggataaaaaa gatccaaaag aatatgctga
     4081 ctggattacc aagcaattta acttgttttc cgatatcgat gagctgtcat ttgtcgatat
     4141 gcctaccatt aaacgaaaca ataaaaccat cgatattgaa acgctcaaag gtaagaagtg
     4201 tgtcggtggt tttgacttgt ccgaaacgga agactttacc gcagccgttt tagaatttcc
     4261 gcttgaaaca ggcgaggtat tcattttgca acacacatgg atcccacaag ctagatttga
     4321 tcgagataac aatcaagagc gtatcaaagc gtgggagaag gtgggagatc taacgattat
     4381 tcctggtgat tacgtcaatt atgaatacgt cttaaattgg tttgtagaaa attcgaaaat
     4441 ctatgacatt gtaaaaatca attatgacaa ggccaaggcg ctacgattaa ataaagaact
     4501 agaaaatgca ggatttgaaa ccaacgagat tcggcaaggg tttctatcat taggtgggcc
     4561 aatgcaaaac ttcaaggaaa tgctattgga cggtaaggtg attttcaaca attccaagct
     4621 ttaccgatgg tatctatcca acgtcaagct ggtgatggat cgcaactcaa actggatgcc
     4681 gtctaagcag tccaagagta gaaaaataga tggttttgca gcaagtttga acagccacgc
     4741 cgaagtgttg aatatgttgg ttaatcctgt cggaaccggg aaagtaacct attactcgat
     4801 ttccgattta atgaatatgt aagaaaggtg tggaggaatg agtattttag atcgtttgcg
     4861 ttcttttggc cgagcgaagc cgaaagcgag caaacaagag tattttttga atgacccggg
     4921 attgataccg tatttagtcg gaaaagatga aatatcagaa gggatttttt ccgtaattag
     4981 ccgtgtatcg aacgtttttg cgtctctccc tctcaaaatg atagatgcgg agtttggcca
     5041 accggacgac tgtcctgcat acaacttgtt gagcgaaggc cctcgatatt ttacaaagtt
     5101 tgattttttc cgggacgtgg aagttttgag aaactaccaa gggaatgcgt atgtgcagat
     5161 tttccgaaat atcaatggag aagtagcaga tatggcgtta ataaaacctg gtgcttgcca
     5221 tccagtgatt gatatggata gcggggagct ttactaccaa gtaactgcga ctgacaaagg
     5281 cagttataag caagttattt atgtacatta catggaaatg ctccacttta aacaaccgag
     5341 gtttggtggc ttggaaggta cagaccccac aaaagtatta acgaataccc tcggatatga
     5401 tcgagaagtc cgaaaaatct ctttaagtca gcttaaagga ggtaatgaag ggctaaaagt
     5461 taagtttgct agcaatgtgg atgaagaagc taaaaaagct acagttaaaa acattgctga
     5521 tttttatcga caaaacggtg gactacttgt ggaagaaaac ggtgtagaaa tcgaacgttt
     5581 acaacgagag ctggtagaca gcaagctttt agatactgat aaagtatctc gctccagaat
     5641 cgcgatggtc tacaacgtgc cggaacattt cattgggagt aaccagtcga gttactcttc
     5701 acaggaacag ctcaatatgg agtttttgac atacaatcta gtaccgaccg ttaatcaata
     5761 tgaagcggaa ctaaataaga aaacactatc gagaaccgaa aaagcaaaag gttatcgata
     5821 taagtttaat gtcgcaagtt tgctaagagc tgacactcag gcaagggggc aattctatca
     5881 aatcatgcga cgaggcggag catactcagc gaatgatgtc cgaagatttg aggacttgca
     5941 accgataaat aaaaccggta tggatgatta tcatatttcc ggagacctat atccaatcga
     6001 tatggatcca acattaagaa aaacaaactc gtctaaaagc gtagccgaaa acggttaggc
     6061 ttttttagtt tgcaccgaag ggaggtgaaa ggatgaaaaa agtgacgtta agcggcgatg
     6121 tcgtggataa cgataccgcg tggttatatg actggtttgg gattgattgt atctcaccag
     6181 ggaaaatttc tgccgctctt acagaagcag cgggggatga agtagaactt gatatctcat
     6241 ctaacggtgg ggatgtccta gcggcaagcg aaatatatac cgctatccgt gcctatccgg
     6301 gaaaggtatc cggaaatgtt gtgagtattg cagcaagcgc tgcaagtgta atcgcttgtg
     6361 cttgcgaacc gcttagaatc tcgccaacag cacacatcat gattcacaac gcatgggtga
     6421 ccactagcgg caacgccgag gaattaaaag ccaatgcgga aatgttaagc agtgtggatg
     6481 agtctatcgt taatgcttac gagatcaaga caggactcga ccggaaaaag cttgctgatt
     6541 taatggcaaa agatacttgg ttaaacgcac aaacagcagt ggcggaaggt tttgcggatg
     6601 aaattatgtt tgcagaagca ccagtaacgg tactcaatgc ctctcaaccg gttattccaa
     6661 aaaacgcggt aactaagttg aaaaatctaa tactcaaagc ggaaacaccg caagaagaaa
     6721 cgctcttgca gaaaaaacta aaagccttaa atggagggaa aaacgaatga atttagaaca
     6781 attaaaaaat gcgtgggtcg aggcgggaag taaagtctct gatttaaacg cgcaactcaa
     6841 tgcagcattg gttgacgatg aaaaaacaga agaagatgta gtaagtttgc aagcacaagt
     6901 aaaagcagca cgggctaaac gggacggatt gaaagagcaa gtggcaaata tggaagccga
     6961 acaagtctta aacgtcaaaa aagaaccatt agataaaaaa gatgaaaact tgaaaaacaa
     7021 gtttatcaaa gactttaaag cgatggtcaa tggtgatcct gctattatgg ctactttgac
     7081 atctaatacg gatgaatctg gtaatgctat cggattgact attcctgtag atgtgcaaac
     7141 gactattcat actttggttc gtcagtttga ctctttgcaa gaatacgtaa acgttgaaaa
     7201 agtgaccact actagcggtt ctcgggttta tgaaaaatgg tctgatatta acccactgac
     7261 cgctttggat actgaagacg gtgaaatccc agcaaatgat gatcctgcac tttacttgat
     7321 caaatacttg atcaaacgct acgcaggtat ttctacagta actaacagct tgctaaaaga
     7381 tactgccgaa aacattttgg catggttgtc taactggatc gcgaaaaaag tagttgttac
     7441 tcgcaataca aaaatcttgg cagctattga tggaatcaaa gcagcgcaaa agaaagatgt
     7501 tacagatgtt gatgggatta aagatatcat aaacgtccaa cttgatccag ctatcgaagc
     7561 tacatctatg tttattacaa accaagatgg cttcaatgtt ttagataaag tgaaacgtgc
     7621 tgatggatct tacttgttac aaaaagacgt agcttctgca actggatata ccttcttagg
     7681 taaaccaatc aagaaaattg cttctcgttt cttgccaaac aaaggtacgc aagctactcc
     7741 taaatatcca ctgtatattg gtgatctgaa agaagctgtt acattgtatg atcgcgaaaa
     7801 catgagcttg ctgacaacga atattggcgg gggggctttt gaaacagaca ccaccaaagt
     7861 acgggttatc gatcgatttg atgtgcagtt agtcgatgat gaagcagttg ttttagctac
     7921 atttacatct atcgcaaacg aagtgccagt aacaaacggt taaggagctg atttcttatg
     7981 attcttgatc ctaaaatgga tttagacgaa atcaaaaacg cactaaagat tgataccgat
     8041 gatgacgatg tggaagtaag acgtgcggtg caagctgcaa ttgcatacat tagaggggct
     8101 atcgggaatg ataagccctc tttttataag caagaaaatg acacggttga tctgattaat
     8161 ttagctattc tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg
     8221 aacttgcgag agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagatat
     8281 ttgctttttg tggaggagta gcgtatgccc cttattcaaa caggaaattt aaatcaacgc
     8341 atcaagtttg tccgagatac gacggtcaag gacgaggacg ggcaagttgt ccggacttct
     8401 acaaccattc ttacttgctg ggcaagtgtg cagacacaac gcctgaacga tattaagacg
     8461 tccattggta cggctttgga aggaacactg acgttcatta tccgctatca acaaaaatca
     8521 gagctaacca atgatatgaa agtgcgttgg aatggaaaaa cgtttgaaat cattacgatt
     8581 acgaaaggcg agtttgcgaa ggacttcacg acaatcattg caaaagaggt ttcaaaatga
     8641 gtgtagaagt cgatgcaacc gaagtgtaca aagcgcttag ggaagtaaaa gcaaacgttc
     8701 aacgagtgga aagcccagca cttagaaaag ctggggagta cgctcaagaa aagttacaac
     8761 aaaacacacc ttactgggat ggaacgaagt caaacggtaa acgtggttcg tatatgcaag
     8821 aacatgctaa ggaccatgtg gttacaagct cggtaaaaaa cggattgata gaagtcggct
     8881 atgataaaga tgtttcttgg cggatgcact ttatcgagtt cggaacaatc aaacaacgtc
     8941 caaaaggttt cgtacaaaaa acacaaaagc aaatcgaaaa acaagtaaca caaatcattg
     9001 ctgacgaagt aaaaaggagg ctaggacttt gaaaacggca gtatcacaag tctattcaat
     9061 tctgaatagc aatgaaaaaa caaagaacat tgatttttac accaatagtg ttccggaatc
     9121 ggctcaaaca gtacctagcc ttccagttgg cagaattaca gagatattcg gcaactatga
     9181 agatttcgca agcaacaatc ctttgaccat tcaatttaac gtacaggtag atgtatgggt
     9241 atcgacctta aaagaggttg atgcctttta ttttgccctt gatgagatta tgagggggaa
     9301 tggttggcaa tgcgcataca cggaacaaac agatgatgag gatttagaag gtgcaaagcg
     9361 gattatcaaa cgatatgtag caaatatttc actaaattaa aaggagagaa aatagatggc
     9421 aacagtagga tttgagagcg tcatttttgg cgtaaaaaca ggtgcaggcg gcactctaaa
     9481 agaattagta gcagataagt cgaaaggcgg agcgatcgaa gctaaaatta ctggattagg
     9541 cgcaacttct aacacaacat acgcttcaaa cgtaccattc ttcattgcaa gtaaaggggt
     9601 ttcgtcgcca aaagttacgc ttgacgtggc agacttaatg gataacggca tttacagcga
     9661 aatcattggc gctaaaaccg tggatggtgt aaatgtaatt ggttcagaaa ctgaagcgcc
     9721 ttacgtgtcg gtagtcatgg ttacagcgaa caaagaagga aaacgcttat tcatgggatt
     9781 ggcaaaagga aaattcagtc atccagatat cgacatgaaa acagctgaag acaaaggagt
     9841 tgaattgcaa accgattcta tcgaagggga attcatttct gatgaacgtg gctatgtata
     9901 cttaacagcc gtagaatcag aagaaatgac cttacaaaaa ttcaaggact tggtaaataa
     9961 caaagcgggg gagtagttaa ccctgcatct acaccaacga cagatacagg gacaccaaaa
    10021 gaaccagaac caaaaattga tacacaaggt tagccatttt ttggctagcc ttattttttt
    10081 gtaaaaacaa ggaggaaaac aaatgattga attgcaattg aaacttgacg gaaagaaaaa
    10141 aacattcaaa caacaagata tttccgcacg tgcaatgcgt gagtgtatca aattttacga
    10201 gaaagcggaa aaagcagacc taactgattt agaagcaatt gattcaatga ttgcaattac
    10261 agcagatatt ttccaagatc cagcagttac atttgatgct attttagacg gtttgactgc
    10321 gagcgagtta gtaccggcat tagaaagtgt ttttgaacaa atcaatgaac tgggaaacaa
    10381 tgaaaaaaag cagacggcga gcaaaaagag ataagttttt ctgaagctag gaaagcaatg
    10441 gatcaaatct acaaagattt aatcgaatca ggttggacga tgagagatgt ggacgaagcc
    10501 gactatcatt atttgttaca cctttttgga gaagtggaga gtggcgaaga atatgtagat
    10561 ggtgctgatt tcatcaaaca atttttatcg gctgaagact tggtaaaact tgaggaagga
    10621 ggtaaataat ggcaggaaaa ggacaaccgg caggaaatat caagctaggg attagtttag
    10681 atagcactaa ttttggtaac acgctggacg aaatcaatgc gaaagtcaaa caagctgagt
    10741 cgaatatgcg tgccaatcta aaggcttatg attcagcagg acgttcatac gaagcactta
    10801 gtcaaaagac gaaagacttg tctacggtta tggaagggca aaacgccaaa gtaagagaat
    10861 taacaaagcg ccgtgatgaa gcgattagca agtatggcga ggaatcgaaa caagttgcta
    10921 accttaacac acagataaac aatgctaccg caaaatataa tgcttacagt cgccagttga
    10981 atgacacaaa aaaagaattg gtgtattcca aaacagccgt caatgattta tctaacgaaa
    11041 tcaaagaaaa tgaacgacaa atgaacgccg aagtaaaagc gttgaaagcc gctggtgatg
    11101 aatctggtgc gtttgaagca aaacaaaaag ggctagccaa acaaacggaa ttatccgaga
    11161 aagctatcga agaacagcgc aaagttgtga aactgatggc tgatgagttt ggcgattcag
    11221 caaatgaaac cgaagatgca aaaagggcat tagaaaagtt agaacgacaa agccaaatat
    11281 ctagcaggca attagaagca ctcaaaagct ccagcgatca atcaggaaaa aaaatagaag
    11341 attttggcga caagtccaca aggtcagcta ggaaactgga tgggctaaaa gacaaattag
    11401 gctcgctaaa aagcgcattt tcgtttggtg cagttgctgg attagcgcat aacgctatta
    11461 gcagtgtagt aagtggcgtg caaggcttgg ttggcgaagc agtaaacgca tcggattcat
    11521 tgatgaagtt ttccaaaacc atggagtttg ctaactttgg gaagtcacag atagaaagct
    11581 cgaaaaaaga aatgaaagac tacgccgata agacggttta tggtttagaa gaaattctga
    11641 acacaaccgc acaattggca tctaacggga ttcctaacta tacagaacta accaaggcgg
    11701 caggtaactt gaatgccgtt gcaggcggtt ctagtgatac attcaaatcc gttgccatga
    11761 tgctaacgca gacggcagga gctgggaaac taacaactga aaactggaat caattagcag
    11821 atgcgatacc gggcgcttca gggctgttac aagacgctat gttgaaaaac ggagcttata
    11881 caggaaactt ccgtgatgca atggcgcaag gtcaaatcac ttctgatgag tttaaccaag
    11941 cgattgtaca gttaggtatg aatgacggag cagttaaagc agctacttcc acagatacat
    12001 tgagtggttc ttgggaacag atgaaatcta ctgtaataaa tgggttacaa agtatcatag
    12061 acaaagtagg cgttgaaaat atcactggtt tcatcaacag agtaacaaaa gggattgaaa
    12121 attctattcc taaaattact caatttatag gttggttgag agatattgga acgtggatcg
    12181 ttgaaaatag agagccacta acatggattg tcggaatcat aggcggaatt acattagcag
    12241 taaaagcatt gaacgtagca agtatgttgc tggcaattac tggcggaaca ttggcagccc
    12301 cttttgtggc gattggtgta gcattaggcg cactagcagg tgctttggtg atagcttata
    12361 caaaatctga aacatttaga aatatagtca atgcggcttt tacagctgtg aaaaacgtag
    12421 ttatgagcgt tatcaataat ttggtggaat actacaaaat gttgtggagc gtgttgcagt
    12481 ggctttggga aaaaataaaa gaatgggctt catggattgg caataaattc attgaaatga
    12541 agaacagcgt tgtgaatacg gttcagaacc tatggaatgg tgtgaaaaac ttcttcagta
    12601 atggcgttgg agacacttgg aataaggtag ttggctgggt aaaaaacatt ttcaacaaag
    12661 caactgaatt gaagaacaaa gtttctgatg caattggtaa cctgtggaac ggtatcaaag
    12721 acacattccg tagaggcatt gatacggtat tcaattggtt ttcagaacta ccgacgaaga
    12781 tgaagaatgc cattattggc ggtaaaaatg ccattgttga tgcgttcaaa agtattttca
    12841 acgcagcact taaagcgata ggtaaaccag ttaacgcaat catccatgga gcttcatggg
    12901 tactagaaaa actgggtgct gacaaactcg aagaatggaa agtgccacaa tacgcaaaag
    12961 gaacaccaaa cggaggtcat ccgggcgggc ctatgatggt aaatgacggt agaggtgctg
    13021 aagcggtaat cacacctaac ggacaagcat ttatcccacg agggcgaaat gtagtgttga
    13081 atgcaccaaa aggcacacac gttctaacag ctgaagaaac agcttatatg actggaaaca
    13141 aagcaccaag atatagatac gccaaaggta caggcttttt cggaaatcta tggaacaacg
    13201 tcaaaggatt tgctggagat gttggaaaca agctgaaaga tgtagtcggc gatgtatggg
    13261 attttgtaac agacccggga gcgttggcta ggaaagtgtt aaatggtctt ggcgtactgg
    13321 aagggcttgt caaatatcct ttagatgttg gtaaaggtat tctaagcaag gctaccgaag
    13381 cattgacgaa caaaatcaca gaactattca gcagtggcag tttagacact tcaatgggca
    13441 tgcaaggcgt ttacaaatac ttggcggacg ttgcagttgc agtaatgaag aagtttccag
    13501 gctttgtggc aactagtggg tatagaccag gtgaccccta ttcgcatggt aaacgtaatg
    13561 ccattgatat tgcactacct ggtgtcacag gaggctcgcc acgctacaca gaagcagcca
    13621 attacgcatt tgagaagttt gcaaacaaaa tcggctatgt tatcacaaat ggcaaggttc
    13681 gtgaccgttc aggacaatca ggtacaggta ttcacaatga ttggagacca tggcccgatg
    13741 gagatcacta tgatcatgtg catttaaacg gtgtgaaaga cccacaaaac actcaaattt
    13801 caggagatag cgtgggaggc agtggggtag aaagatggcg caatgtagca attagagcgt
    13861 tgaaaatgac cggtcaatac agtactgcaa acttaaatgc attactaaat caaatgcgta
    13921 cagagtcaaa tggtaatcct aatgcagtta acaattggga tattaacgcc aaaaatggaa
    13981 caccatcaaa agggttgctc caagtgattg acccaacatt cagacagtat gcaatgccag
    14041 gattcaacag caatattttt gacccactat ctaacatctt agcttcaatc agatacgcac
    14101 tatcaagata tggctcacta acaaatgcct atcgtggagt tggttacgca aacggtggaa
    14161 ttgtaaacca acatcaaatt gcggaaatcg cagaaggaaa caagccagaa attattattc
    14221 ctttagataa ggctaaacga tcaagagcga tgcaattatt gtctatcgca atggacaaac
    14281 taggagtttc tcctaaaact tatggaaatg cgacaactgt ttccagtgat tacgaaatcc
    14341 tagaagccat tgaaaaacag gcggcaacaa cgaatcagct gttatcgtta ttgctggcat
    14401 ttttcaaagg aaatagccga actgatagag atttagcttt agatattcag aagatcttgg
    14461 ttaggaggat gtagatgcga actgtattac tgagaaatag aataaacgaa gaaattgatt
    14521 tgtctacgga ggattatttt gctactgggt tgagtaatat ggggtttgaa gtaaaaaaag
    14581 aacatgtggg acagtggggg aatttcagag aaagcagtga gaacgttgaa atatctgaat
    14641 ttcagtcatc tgtaatcatt tctgtgcatg ggtttcgaga aaaagaactg tacaattcac
    14701 ttgtgcagtt cctatcggaa ggtccgtttg aactggaatt tgcttttgac ggcgaaacaa
    14761 tggtaagaag atgcagtcta aaatctttaa gtaagactga aatcgatccg aaaacatcgt
    14821 tactaacaga cactttagaa ctatatttca catcaaactg gtattccgta aaacgcgaaa
    14881 aattaattca gcgaccgaat gtagtgaaaa cacgtggtaa ggtttttccg tataaaagat
    14941 cttatatcta cactcaaaac ttgtgggaga aaaaaggtgt attcaaattc aataataatt
    15001 ctgtatacct aacgaatagc aaggaacgga tgtccccgct aaaaattcgt gtgattggga
    15061 agtgttcaaa tccgtattgg gaagtaatcc aaaactcaca aatcatcgca acggatggat
    15121 acttcataga tatgactgaa acacagactc tagaagtatc aagccttttt gaagatacaa
    15181 cggcgatttt aaaagatatt gcaggggtag aatcttctgt ctaccaacaa caggattata
    15241 caaaaactaa ttttgttcag gccccgactg gagaattcag cattgtgttt catgttgggg
    15301 gggcagacgt agagattgag ttatacgagg agcgtgatct gttttgattt tagcagtcac
    15361 gctttttcat cgtgatttaa tgctttataa cgaacattta ttctctaaca gttttgaatt
    15421 tggtgcggac gagatcaacg aagaggctag tagctttacg atggataagt acgttcctgt
    15481 aaaaacaggc gattttctac tagcaaaata tattcctagt ggaaaatttg catattttgg
    15541 tgtaattacg tcgcaggaag acgaaaaaat cagttgtaaa agcttactta gtttagctga
    15601 tagtgagata ccgactgcac gtgtgtcagg agataactac gaagagcata tccggcggtt
    15661 gattgaatac tatttgctga atgatccaac gaaacaacta aaagatattt tagacgtcaa
    15721 agcagaaagt gcgacctctc attcgtatca agctaccgat acgaataaac acaagttaag
    15781 tgcgtatatt ctcaatggtt tcaaaaaata caacgtaaaa tggtatttca agggaattca
    15841 aaacagaaaa atctatacag gcatacgtgc tgtaaatgaa tcaatttaca ttaaagacaa
    15901 ttcttctgaa tttagcgatt gggatgtgtt tgttcaggcg ccgggcgctg gaaacgagaa
    15961 caagctatta atcgttgata aagcaatgaa agatatagag aaaccgataa tactgtcaac
    16021 atggtatttg gacgaagaaa acaatttgac acaagatgga tcgaaagaaa atatcacgaa
    16081 accaactgtc aatttggtta atatctacga tcaaaccgca gaagataaag catcctacga
    16141 agatgtggca aaatcagagt tgaaaggcaa tacgtattcg catgaaatca aagtgaacgt
    16201 tgtaagaaat gcaaaaaatt tgaatgtcga aacgattgaa acagggatgt ttgccacgat
    16261 ttcttataaa ggaaagatat acaagtcggt tttgacagct tggcgaatat caagtgataa
    16321 ggagtttgtg gaattgactt tcggaaacat aagaagtcgt tttatggatt attttgaaga
    16381 taatggggga taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt
    16441 aagcgcagaa aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat
    16501 tgatggttac gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc
    16561 aggagcagcg attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt
    16621 tccagcaaac tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc
    16681 tggatctatt cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga
    16741 atttataaca aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc
    16801 actatgctca gttacttcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta
    16861 cgagctgact ctctctaaag gagaaatttt gtggagtggg actgcgttaa tgcatgatac
    16921 tcaaactgtc caaccttcaa aaaaaatgtg gcagacagtt tcaggttttt tattagtatg
    16981 gctcccatac gaaaacgggc aagcaattgg ggatagatat gtaactacgc ctttttataa
    17041 agagcgtgta acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca
    17101 caaaaagtgg tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc
    17161 agcaagcaat gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta
    17221 gttaggaggt atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag
    17281 ccttatctac gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat
    17341 gtacaacttc ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg
    17401 aaatttgtgg gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat
    17461 tcgactaatg ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa
    17521 ttcaaacaag catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac
    17581 ttcatcatag acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt
    17641 tcttcattgg aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat
    17701 aaacaggtcc aatacgatca aatctactcg aaatataacg aattagtaag attgataaat
    17761 gaatcagata agcaagtaaa tgatcgtatt gataagacaa atgatcgtat tgatcaaacc
    17821 aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag catcgacttc
    17881 ggggactatg attatagtgg gaatccgaac cttagcgcta aacttaatgc ttcaagtttt
    17941 tctttaggta ctggcgcaac agtcgcggat gataatgatg agattgtgtt taccttagat
    18001 ggcacaaatc aactatcgaa gtacgctagc aaagcgcaag tgccggtagc agaagggaaa
    18061 caatatacga ttagttgtga aattatgtta gaagatgggt tcactggcga cccttcagga
    18121 atacgtctgc aacacgcata tttgccaggc ggggttgttg ttttacagac agagacagta
    18181 cctaaaaatg aattgaatac gtggcagacg cttatcggaa cgcgaacggt aaaatacacc
    18241 tctgacgtac caaatgaatg gtaccctttg tttagagaca tccgaacact taaaccgacc
    18301 ggtaaagtta gactaagaaa aattaagatt gaagaaggct caacagccac cccatatcag
    18361 ccaaacttac ttgatgaccc ttactggcta ggtaaagcgc ctttgggtga gaatattgct
    18421 aataagtctg ttacgtttcc aatcaaatct agcgcctaca gcctatacca agctaacatg
    18481 gaagaagaat ttgttttagg tcaaacctat acaattacca tgaaggcaac caaaccacct
    18541 atccaaacgt tcattgttta caacgaggat agtggagatt atagatatgg taacttagag
    18601 ccagtagaag ggttagttga tacatggggt ctgactttta caccgcagaa ggttggagtt
    18661 aactacccca aacggttaac tattatccag tatccccaat caacaacagg tgcatgtcaa
    18721 attgactggc tcaagatcga aaaaggcaat actcgaaccc cgaatattag tgagtataaa
    18781 tatcgtggta ctggtatgcg tgattcaaac aatccaaaag attatgtttg ggatctagca
    18841 ccagaatatg tcgaagataa tttggccaca gatattaaaa tttctgaaat tactggtaaa
    18901 gcaaacaatt ataccgatgg gaaagtatcg gagattaatt cgcagttgac tgcttcaatt
    18961 aatgaagtag acaccacagc taaggatgct caaacaaaag cgaatgctaa tgcgactgct
    19021 atagatgaat tagacaataa gatcgatgaa cgcattaatg atacagctac taccacatta
    19081 acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa aacagtttct
    19141 atatattttg tggctttaaa cggaaaaagc agtggtggaa atgattcaac gatactaaca
    19201 attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt
    19261 tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga
    19321 aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta gaaaagcgtg
    19381 aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt
    19441 gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga ttctaaattg
    19501 gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggacgt
    19561 actggcggag agtacgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca
    19621 gatcagccac cgcttgaagg aattcgtgct gatggcgaac cgttaacaat cgctgatcaa
    19681 atgtttgatc ctaaattgaa tcaatggatt attttagcga acgcactaga tcacaacgat
    19741 ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag
    19801 atcaatgcca aactcatgct aagcgatgta gcaattaaac aggaaaatac tgcattgaaa
    19861 gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat
    19921 agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag
    19981 tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca
    20041 agttcgacta ttcgttccac tatgcattac agacgaagaa gcagataaaa tcattagcaa
    20101 agaagaaagc gcatcttaag tgatgcgttt ttatttaagg taaaggagtt gtcacatgat
    20161 caatttaggg gaatggggaa caatcgcagg atcaattact gcgattgttt ctttgatttt
    20221 attagtaata agaccagtca ctgcatcttt ctcgaagatt actgaaactc tttcaaaagt
    20281 aagtcacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat
    20341 gattcatgaa gaactaaaga aacacgatga aagattagat acacatgcag aaaaattggt
    20401 agaacacaca caacaaatta aaactttatt tagagaaagg tctcggtaaa aaagaaagga
    20461 gttaagaaga aatgatttta cccgataagt attatcaagt cattaaatgg acagttttaa
    20521 cagtattgcc agctttatct gtattagtag ccacattagg caaagcatat ggatggaatg
    20581 gaacagatat ggcagttctg actatcaatg cagcagcaac atttttaggt gttatcactg
    20641 gtgtgtcggc ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt
    20701 actgcgatga gcctattaac ggctcttttt ttattgccga ttaatgggtt tgcctatacg
    20761 attaacaatg aatttaattt aggtgtaaac gaaggtagtt ctcaagtagc aaataatcag
    20821 tacattttac tgcatgaaac ggctaatgaa acagcaacag gacgcaatga agcgcagtat
    20881 atgcaacgtt catggactag cgcttatact gcttatattg tgggagacgg cggaattgtt
    20941 tatcaagtcg gtcaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgcaaat
    21001 agtcctgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
    21061 aaggcatacg ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta
    21121 gacacaccgt ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg
    21181 ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
    21241 gcctatgatt tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
    21301 gtcattgatc caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac
    21361 gcccacattg atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac
    21421 atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta
    21481 gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta
    21541 ggaaatgttg gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat
    21601 gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
    21661 catgataaac gctggtattt aaatattccg caacgataaa aatagcccct cgtttttgag
    21721 gggaagtaca taatgcaaat ttgtccataa cttattgatt atcttgtaaa aatgcggtaa
    21781 tttaatagtt agtgagaaga ggtgcttaat tatagttaaa aaagaggaaa tagaagcagc
    21841 atgtaaccaa gcaaatgtta ggtttattgg agaagcttta tctggatatc ctaagtataa
    21901 atacttacac aactggttac aaagtaaaag tttttatctt aataaagaaa gatgcatgcc
    21961 taataaaaat tatagggttt ataaaagagg aactattgta tatgttgatt ttggggtgaa
    22021 cgtgggttat gagttatctg gtaatcattt tgctattgtt ttaaataata aagataattc
    22081 gaaaaatggt gttgtatgtg tagtaccaat ctcttcaaaa aagaaaagta attatgtatc
    22141 tgtaggaaaa a
//
LOCUS       DAYYAG010000039        21813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000039 DAYYAG010000000
VERSION     DAYYAG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.039"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHARQ_001280"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHARQ_001280"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8409709.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHARQ_001281"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHARQ_001281"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8409710.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHARQ_001282"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHARQ_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8409711.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHARQ_001283"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHARQ_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8409712.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHARQ_001284"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHARQ_001284"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8409713.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHARQ_001285"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHARQ_001285"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8409714.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACHARQ_001286"
     CDS             complement(8557..9027)
                     /locus_tag="ACHARQ_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8409715.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACHARQ_001287"
     CDS             complement(9073..9498)
                     /locus_tag="ACHARQ_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8409716.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACHARQ_001288"
     CDS             complement(9565..9834)
                     /locus_tag="ACHARQ_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8409717.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACHARQ_001289"
     CDS             complement(9845..11440)
                     /locus_tag="ACHARQ_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8409718.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACHARQ_001290"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACHARQ_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8409719.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACHARQ_001291"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACHARQ_001291"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8409720.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACHARQ_001292"
     CDS             15906..16880
                     /locus_tag="ACHARQ_001292"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8409721.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACHARQ_001293"
     CDS             17050..18450
                     /locus_tag="ACHARQ_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8409722.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACHARQ_001294"
     CDS             complement(18528..19250)
                     /locus_tag="ACHARQ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8409723.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACHARQ_001295"
     CDS             complement(19329..19835)
                     /locus_tag="ACHARQ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8409724.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACHARQ_001296"
     CDS             19958..21757
                     /locus_tag="ACHARQ_001296"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8409725.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6053 a         4752 c         3911 g         7097 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ttgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttg
//
LOCUS       DAYYAG010000040        21789 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000040 DAYYAG010000000
VERSION     DAYYAG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.040"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..2000)
                     /locus_tag="ACHARQ_001297"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="ACHARQ_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="ACHARQ_001298"
     CDS             complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="ACHARQ_001298"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8409726.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3230..4519
                     /locus_tag="ACHARQ_001299"
     CDS             3230..4519
                     /locus_tag="ACHARQ_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8409727.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4558..5163)
                     /gene="ytpR"
                     /locus_tag="ACHARQ_001300"
     CDS             complement(4558..5163)
                     /gene="ytpR"
                     /locus_tag="ACHARQ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8409728.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5368..5832)
                     /locus_tag="ACHARQ_001301"
     CDS             complement(5368..5832)
                     /locus_tag="ACHARQ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8409729.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5854..6174)
                     /locus_tag="ACHARQ_001302"
     CDS             complement(5854..6174)
                     /locus_tag="ACHARQ_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8409730.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6318..7397)
                     /gene="pepA"
                     /locus_tag="ACHARQ_001303"
     CDS             complement(6318..7397)
                     /gene="pepA"
                     /locus_tag="ACHARQ_001303"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8409731.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7597..7947
                     /locus_tag="ACHARQ_001304"
     CDS             7597..7947
                     /locus_tag="ACHARQ_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8409732.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8170..9117
                     /locus_tag="ACHARQ_001305"
     CDS             8170..9117
                     /locus_tag="ACHARQ_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8409733.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9174..10592)
                     /gene="pepV"
                     /locus_tag="ACHARQ_001306"
     CDS             complement(9174..10592)
                     /gene="pepV"
                     /locus_tag="ACHARQ_001306"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8409734.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10741..11586
                     /locus_tag="ACHARQ_001307"
     CDS             10741..11586
                     /locus_tag="ACHARQ_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8409735.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11590..12030
                     /locus_tag="ACHARQ_001308"
     CDS             11590..12030
                     /locus_tag="ACHARQ_001308"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8409736.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12080..12274
                     /locus_tag="ACHARQ_001309"
     CDS             12080..12274
                     /locus_tag="ACHARQ_001309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409737.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12410..13087
                     /locus_tag="ACHARQ_001310"
     CDS             12410..13087
                     /locus_tag="ACHARQ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8409738.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(13495..14184)
                     /locus_tag="ACHARQ_001311"
     CDS             complement(13495..14184)
                     /locus_tag="ACHARQ_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409739.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14159..16288)
                     /locus_tag="ACHARQ_001312"
     CDS             complement(14159..16288)
                     /locus_tag="ACHARQ_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8409740.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16336..17673)
                     /locus_tag="ACHARQ_001313"
     CDS             complement(16336..17673)
                     /locus_tag="ACHARQ_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8409741.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17925..18284)
                     /gene="rplT"
                     /locus_tag="ACHARQ_001314"
     CDS             complement(17925..18284)
                     /gene="rplT"
                     /locus_tag="ACHARQ_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8409742.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18337..18537)
                     /gene="rpmI"
                     /locus_tag="ACHARQ_001315"
     CDS             complement(18337..18537)
                     /gene="rpmI"
                     /locus_tag="ACHARQ_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8409743.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18609..19133)
                     /gene="infC"
                     /locus_tag="ACHARQ_001316"
     CDS             complement(18609..19133)
                     /gene="infC"
                     /locus_tag="ACHARQ_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8409744.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19177..19302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19453..20085
                     /locus_tag="ACHARQ_001317"
     CDS             19453..20085
                     /locus_tag="ACHARQ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8409745.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20087..20992)
                     /locus_tag="ACHARQ_001318"
     CDS             complement(20087..20992)
                     /locus_tag="ACHARQ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8409746.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21077..21736)
                     /locus_tag="ACHARQ_001319"
     CDS             complement(21077..21736)
                     /locus_tag="ACHARQ_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8409747.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
BASE COUNT         6485 a         4420 c         3943 g         6941 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 ccccgcattg atccggaagg cacggtagat ttgctcgaca aggatcagac gcatcaattg
     2401 atgtggatac gtcatttttc caaaagagat ctgagcattg cttcttttca tgacggcttc
     2461 gctgagtcct aaagagccgc cgataacaaa aacaaggtgg ctttttcctt ggatacctaa
     2521 ttggtcgatt tgtttagcaa aagattcact tgtaggattt ttcccttcaa ttgctaaggc
     2581 atagacgtat tcttgttctt tgattttcgc taagatccgt tccccttctt tttcttttac
     2641 ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa cttcgatcaa
     2701 ttcgattttt gcataggcat tcagccgttt gacatactca ttgatacctt ggatcaggta
     2761 tttttctttt aattttccta ctgagataat tttgatattc atgttttctc tcctttggtc
     2821 ttcatgctca ctttgtttat tataatcaat tttttcgtct atatgtttat tttggctatt
     2881 tttgttaaaa agatttcccc aacagttatg cacaacatcc acaggtttat ccacagaaaa
     2941 cagtgttatt ttaccttatt tcatcacatt cattaggcaa acatacgttt tataagaatt
     3001 tatccacttt atacacaggc tgtggatatg ttttatccct tatttttcca atcctatccg
     3061 cgtagtttta cacagtatct gtggaaaaag aaaatcaaat attttcatct gaaaacaatg
     3121 atttttccta aagtttttca taaaaagttc acagtctcaa agtagactaa agtcaacaaa
     3181 taagactata ctaacagtag ataaatgaat gtaattggag gaggaacaca tggatagaaa
     3241 aaatgtgaca ccaaagatga aaaaaagtaa aaataatagc ctctggcgta agttaggtct
     3301 ggggcttgtt ggagggatcg ttggaggact actgacagct ggcatttttt atgcagcgat
     3361 gggcactggc aacaatagtt ctactgctac aagcggcaat cagaattctg ccggtgaaac
     3421 agttgtggaa aatgtcaaag tcaatgtaga ttctgatatc accaaagcgg tagataaagt
     3481 tcaagacgct gtcgtttctg ttatcaactt acaaagtcaa aaccaatcaa gcggttttgg
     3541 cggactattc gggcagcaag aagaaagtag cagctcagat gatagtaatt tggaagctta
     3601 tagtgaagga agcggcgtga tctacaaaaa agacgggaat actgcttatg tagtcacgaa
     3661 taatcacgta gtagatggcc agcaaggtct tgaagtcttg atgaaagacg gtacgaaggt
     3721 aaaagctgaa ttagttggaa cagatgctta ttcagattta gcggtgttga agatcaattc
     3781 agacaaagtc gaaacagttg cctcttttgg tgactcaagt gctctgaagg ttggtgagcc
     3841 tgcgattgcg attggttccc cactaggatc tgaatatgca aattctgtca cttcaggaat
     3901 catttcttcc ttgaaccgac aagtgacaag tacgaatgaa tcgaacgaaa cagtcaatat
     3961 caatgcgatc caaacagatg cagcaatcaa ccctggaaat tctggtggtc ctttagttaa
     4021 tatcgaagga caagttatcg gtatcaactc aagtaagatc gcaagtactt ctgaatctac
     4081 ttctaatgta agcgtagaag gaatgggatt tgctattcca agtaacgatg tagtgaatat
     4141 catcaatcaa ctggaaaaag acggaaaagt gacacgtcca gctctaggta tcacaatggt
     4201 cgacttatct gccgtttcta ctcagcaaca agaacaaatc ttgaagattc cttcatctgt
     4261 aactaacggt gtcatcgtga caaatgtcca aacagctaca ccggctgaaa aagctggatt
     4321 gaaacaatac gacgtcatta ctaagatcga tgacaaagaa gtttcttctg gtgtagaact
     4381 tcaatctgct ttgtataaga aaaaagtggg agattctgtc aaaatcactt actatgaagg
     4441 ctcgaaagaa aaaacaacta ctgttgaatt gacgatcgat caatcggcat taaaacaaaa
     4501 tcaaaatagt gaaaattaat aaaaaagagg ttgtgacaaa gacttgtcac aacctcttta
     4561 ttttggaaat gcttctccga ctacggcatc ctcaggcaat actaagattc ctttttttgc
     4621 tggagcattt ggcaaatgca attcacgagc ggaacagatc attccatagc tatctacccc
     4681 tcgcaaagtt cctggccaga tcatcattcc atctggcatc atagctcctg gttttgcaac
     4741 gacaactttt tgtccagctt cgatattggg tgcaccgcaa acgatttgca atactgtacc
     4801 attgtctact tctgtctcag taatcgataa atggtcagaa tcaggatgtg ggacgcatgt
     4861 ttttacgtac ccgacaacga tcttcggttc atgatcagct tcaagctgtt cttcgaatcc
     4921 tgcttctttg atcaatcgat tcaatttcgc taattcctca tctgttaagc ttacttggcc
     4981 gttcccttcg attgttagat gatcggagat atggaaaaag ttccaagcaa caacttgacc
     5041 ttgttcatct ttgatccgag caacattcca tttgcgttca acagtgtttt ctgttccttt
     5101 atcgtcggct ataataacca tcaaggtgtc tccgacgtgt gctttgttat aagcaaaaat
     5161 catgttcttt cctccattat cctttgctga gatttatctt aacaaaaaat tgcattcact
     5221 gcatgaaatt tttttcatgc tttatttccg aagaagcctc ttctggaaca ccatttattc
     5281 gtaacaaggg actgtgacaa gacttttgtc acagtccctt tactgtttta gttaattgat
     5341 taaaaaggtc gtacaagttc cacttgctta attacttggc tgttcttctt cgtcagaaac
     5401 gatcaagaca tcacaaggtg aatgacgtat cacgtagctg gctacacttc ctgtcataaa
     5461 gcgttcgact gcatttagac ctgactgccc aaccatgatt agatcgatct catatttttt
     5521 tggcaatttg actgccatgg attcacgaac agaaccatat gcaataattc cttctatatt
     5581 tgtgtaatca acagatttcc catattcttt acattcttcg atcatttcct tggctgtttc
     5641 agtttgctga tcgactagat tttgatcaat aggtgcatag cccataaagt tataaaattg
     5701 ctgatcgatt acgtttgcta catatacttt cccattatta cggcgagcca cttctaccgc
     5761 ttttttgaaa gcagcaagtg cttgttcact accgtcgata cctactaaaa tattcttata
     5821 ttcttgattc atcttgaccc ctcctaacag atgtcagcct tttgttacac tttctaggaa
     5881 cgattcgatt tcctgttttg ttttacgatc tttattcact agacgaccta attcttttcc
     5941 gtcttggatc acgacaaaac ttgggatacc aaaaatgttc cattcagcgg ctacatctat
     6001 gtacttgtca cggtctactt caataaattg ccaatcagaa aagtcatttt caatttcagg
     6061 catttgcggt ttgataaaac ggcagtctcc gcaccagccg gcagtaaaga aaaatacact
     6121 ctttccagtt tctacgtagc ctgctaattc ttctaaacta tttgggataa tcattctttg
     6181 tcctctactt tctgtttatg tatttttcaa ttaaattata cacctttctt tcgaactaac
     6241 aaacattgat cctcttttta taaaaaaaat ctggcaaaaa gcaatctttt gccagatttt
     6301 atagtttcac tatcgcatta ttttccgtat acgatcgtat ttactgttga tttatccaaa
     6361 cctttcagtg tttgaatcaa catttctcgt gcagcttcaa aatcgcgaat actgaacatc
     6421 gtttgatgcg tatggatata acgtcccact acgccaatca ctgtacttgg gattccttcg
     6481 ttttgtgtat gagcagcacc tgcatctgta ccgccttttg aaacaaagta ttgataaggg
     6541 atattgtttg tttcagcgat atccagcaag tactcgcgca aacgaggaag catgatcaaa
     6601 cctggatctt ggatacgcat caatgtacct tctcctaaat gtccgaatgt gccatttttc
     6661 gtcactgtat catctgctgc agagcagtct actgcaaaga acagatctgg cttgaattta
     6721 ttgacagaag ctttagaacc acgcaagcct acttcttcct ggacattcgc gcctgcaatc
     6781 aatgtatgac ctaatgtttc attttgcaac gcttctaaag cttctaatac cattgtacag
     6841 ccatagcggt tgtcccatga tttactgata atattctttc cgttggctgt tttgatcgtt
     6901 tcagcgtaag gaacgattgt gtctcctgga aggacaccgt aactcattgc ctcttctctt
     6961 gattcaaacc cagcatcgaa caaaatatcg gttacttcta ctgatttttg tccgcttgtt
     7021 cctcgcaata aatgtggggg aacggaagaa gaaatacatg gataattctt accagtagag
     7081 gttttaagtg taaaacgctg agcagaaaca acgtaaggat tccagccgcc tagaggtacg
     7141 actttaaaaa gcccgttttc ttggatttgt gtcagcatga atccgacttc atccatatgt
     7201 gctgcgacca ttacacgggg tgcatccgct tcttcatgat ggcgcagacc aaaaatgccg
     7261 cccaaaccat ctaactgtaa ctcatctacc agcggttcca tatgactttc catataagcg
     7321 cgaacatctt gttcaaaacc gcttgttcct tgtaattctg ttaactcttt gattcgttga
     7381 aatgtttttt cttccatatg ctattcgtct aaatttcaga ctaaactcct ctcagactta
     7441 tattgatctg cggataaaat atgatttttt ctgcagaccg atcgtattta ttatagctta
     7501 ttttttctgg tagcggttat ttatttcaaa gaaaatatga gagtgtggta aaataagcac
     7561 aaggagtaac aagtgagaag aaggaggtat gtccacatgt atgaagaaag cgaactaagc
     7621 tattttaaag gcgggttagc agttggaatc ggtctaggat tattgagcgg ggtagcttca
     7681 acgatttggt atcatcgcaa aaaagtattg tcagcagacc aagtacttga aaatatcaag
     7741 tcatcttttc taaaagaagg tgcaatcgaa ggttcttgga tctcttttga aaaggaacct
     7801 attaggaaat tcgccgttcg gtcgagtgct tatcggggag gtctttctcg aatagaagat
     7861 ggcgaaactg tttattatga atttttagca gatgctcata caggaaccgt attagaaatt
     7921 tcaagaaaaa aaggtgcaac agattaatac gtcaaaaaag tgagagaacg aatacgcacc
     7981 cactattcaa aagagaggta tcatgtcctt cttttgtagt gggtggtttt ttagatagag
     8041 ctttccaaac atatattcca gtattttctt tttaaataaa tgatatttag cgggaaaaac
     8101 tagcatttcc tttgattatg aggtattttt aatttagctt ttgttttcta tagttaagga
     8161 gagtgaaaaa tggaacaaca agaaaaaatt gctgttctga ttccttgtta caatgaggaa
     8221 gcaacaattt caacagtcat tgcagacttt aaacgagaat taccagaagc agatatttat
     8281 gtctatgata ataattcgac cgatcgaaca tacgaattgg ccgttcaagg tggagcaatc
     8341 gtaaaaaaag aacctcgtca aggaaaagga aatgtgatcc gccaaatgtt ctttgatatc
     8401 gatgccgact attatttaat ggtagatggc gatgatactt atcctgcaga agctgtacat
     8461 ggtttgctcg agaaattacg ttctggtgaa gcagatatgg tcattggtga tcgcctatca
     8521 aatggaacct actttgatga gaataagaga cctttccacg attttggaaa taaccttgtt
     8581 agaaatacaa ttactagaat gtataaaaca aaaattcttg atgtcatgac cggctatcga
     8641 ggattcaatc gtatctttgt caaaagtttt cctattatga gttctggttt tcaaatcgaa
     8701 acggaattga cgatccatgc tttagataaa aaattcaagt tcgttgaaat cccaatcgat
     8761 tatcgtgatc gtcctgaagg cagcgaatca aaattgaata cattctctga cggattcaaa
     8821 gtgatcatga tgattattaa aatgtttaaa gattataagc ctttgctttt ctttgggatt
     8881 tggaccatct tcttcttttt atttggattg gtcacgggga ttcctgtgat tcgcgaatat
     8941 atgctgacaa gctttattac acgcatccct tctgcgattt taagtactgg attgatgact
     9001 ttagcacttc tttcattagt aacagggttg attttggata cagtcgtcac gaacgctaag
     9061 aaagaatatg aattgaatct atatcatgtt tatcatgaat accacagtag aaagtaaaaa
     9121 aaggatcaag actgcaaaaa taatttgcaa ttcttgatcc tctttttaat taattatttc
     9181 gccaaacgat agatggcatc tgcatagatt gctgttgctc tgaacaagtc atcaagactc
     9241 ataaattcgt ttgcttgatg cattgtatca gtatatcctg ggaacatcgc tccataagca
     9301 acgccacgtt tcaacaaacg tccgtaagtt ccgccaccga tgatttgttc gtagcctttt
     9361 tctccagtgt ggtcttcata gacttgcaat aatgtagcca ccaatggatc atccattggt
     9421 acgtaatgtg gttccattac gcgagcacct cgagtaactg ttgcgccttc ttgccccact
     9481 gtttgttcga tgtcactttg caatccgtct actgttacac cttttggaaa acggaaattc
     9541 atattgatga agttcgcttc ctctgtacca ttttcttcaa aagcaaatag tccagcattc
     9601 attgtcaatt tgcccatttt ttcatcttca taagcgactc ctagtttttc tccataaaaa
     9661 tcttcatgga cataagcagc tgctacatgg ataaattgtt tagcagatcc taggaattca
     9721 tagttatcta agaacaatgc caagaaagaa cctgcattga taccagattg tgggcttgca
     9781 ccatgtgcgc ctttaccaac taattcgatt tttacatacg tattgtccgc ttcaatcgtt
     9841 ccgcttactg gatttgcttc gatgaattgg taaaatgctt cttccatcgc gatagctgca
     9901 tctgcacttg gtacttgaag tgctgcagtt gctgttcctg gcaccatatt ttcgcgaagt
     9961 ccggaaacaa aagatttcaa gacataatct cctgcattgt cgcctttgaa gcgtaaagct
    10021 aaagaaacat ttcctttttc accattgatg attgggaatt cagcatctgg tgagaaacca
    10081 aaatctggtt cttcttcatg ttcgaaataa taagccatat cgccccagcc gctttcttca
    10141 tcgctgccga ctacgaagcg aacttttttt gatagtttca agtcaagttc tttaatgatc
    10201 ttcatcgcat agtaagctgc catacttgga cctttgtcat cgcttgatcc gcgtgcaaag
    10261 atctttccat ccttgattac tggttcataa ggatctgtgt cccagccatc tcctgcagga
    10321 acgacatcca tgtgtccaaa tatccctagc gtttcgtctc cttcgccaag atcaatatgc
    10381 cctgcatagt tatcgacatt ttttacttta aatccatcac gttcgccata agctaacata
    10441 tgtttcaatg cgtcgcgagg tccaggtcca aatggtgcat ctggtgttac ttttgagtca
    10501 tcgcgctcgc tgttgacacg taaaagattt ttcaagtctt caagcaagtc ttctttacgt
    10561 gcttctactt ctttttgcca atcaattgtc ataaagttaa tcctccactt atttaagtct
    10621 tcgtgttcat gataccacaa attacagaaa aaaatagttc aaaaaatctg acaattcctt
    10681 ttcttatctg tttttctcta aaaaaaatat acgctataat gagtaaaagg aggtttttag
    10741 atgctgataa acgaacaact agtgaaaaga gtcatcacac ctcgtccaaa aatttcacat
    10801 aaaggaacat ttggacgtat cgttttgatt ggcggcaatg aacaatatgg cggagcaatc
    10861 attatgagtg ctttagctgc cgtacacagc ggagcgggtc tcacgactgt agtaactgat
    10921 gaaaaaaatc ggagtgctct tcacagtcat ctgccagaag caatgattgt agtttggacg
    10981 gatactgacc agtttctttc cgtaataaag caagcagatg tactcttgat tggtcccgga
    11041 ttgggtactt cttctaagag tttggaacgt ttcaaacaag tactagaaaa ccaatcaaaa
    11101 gaacaatggc tggtgattga tggctctgct ttaagtttac ttgctgctaa tcatcttttg
    11161 ctaccctatc ccgaacaaac tgtacttaca cctcatcaaa tggagtggca acggttgagt
    11221 tctctaccga tacaagaaca acgggataaa gaaaacaaaa agaagcaaca ggaaatcggt
    11281 gctgtgctcg ttttgaaaag tcatgagaca aaaatttatg gtcaccagca cttctacacc
    11341 aatcctttag gttctgctgc tatggcaacg ggcggcatgg gtgacacttt agctgggatg
    11401 atcacaggat ttttagccca gtttcctcaa aatgaccaaa cgattgcagc agctatttat
    11461 cttcatagtt tcattggaga ccagctggca aaagaacaat atattgtttt gccgacacaa
    11521 atcagcgaag cacttccgaa atggatgaat tatttttgta aaaaatcaat ggaaaacgag
    11581 gcatgagtca tggagaaatt tacaggttat gtgcgagaac ctttttatta tgaaacagat
    11641 caaatgggga ttattcatca cagcaattat atccgctggt ttgaagaggc tcgggtagcc
    11701 ttattggtac atctagggtt tccctataaa aaaatcgaag aatctgggat catcatcccc
    11761 gttctggaag taaattgcag ctataaagaa atgatccatt acggagaaaa agtgagcatc
    11821 catccaacta tccaaaagta tacggggaca cggttggatt ttagctacga aattgtggga
    11881 actgaagaca aaaaattaaa aactactggc agtagtaaac actgtttttt actttctgat
    11941 tcgcaacgat tagtgaagct gagcaaagtc aatccggagt tagatcagtt attccaagcg
    12001 tacgcttctt tggatgatgt aaattcttaa taaagaccgt ctaagcattt ttcttgtatt
    12061 gtgtcgagta gttcgtataa tgaaaagaaa aaagcaggtg atccccatgt atgtcgttaa
    12121 agtaatgcac ggttacatcg ataaaactgg ctgtcgcact cgggaaaaaa acttagacaa
    12181 tctgctcatt ttcaaagaca aaaaagaatc cgaagctttt gctaaaagaa tcggcggtcg
    12241 tgtcaaaccg atccaagagg tacgaccaga ttgattcaat atttttcttt tcgaggctct
    12301 aataggcatg cctatcaggg ctttttttgc agactgtgtt tttatgccca cacgatttgt
    12361 aagcgattta tgttataata gattatatct gcatagaaag gaaggttaaa tggttatcaa
    12421 taatttgcaa cagcaagctt atgaatctat acgtaaacaa atcatttatt ccgagttagc
    12481 acctggaagt aaaatctccg ataaagtgtt agaagaacaa ctagctatcg ggcgtacacc
    12541 gattcgagaa gcactgatcc aactgcgcaa tcaagagctt gtaaaaacca ttccacagtc
    12601 cggcacttat gtttcaaaaa tcgatatccg atctgcttca ttagcccgtt atacgcggga
    12661 aaagttggaa aaaccgatcc tgcaagaatg cagtgcgaaa atgaccaagc aagatcaagc
    12721 taaactagaa aaaatactag aacaaaccga tcaggcagta atcgctcaaa ataaaaaagc
    12781 tttttttgat ttggatactg attttcaccg aacttgttat gaaattgctg gaaaacgaga
    12841 aatttgggat tggctagaaa gttatagtac gcgtttcaga tggttacgtt tgacgatttc
    12901 agaattagac tggggtcgag tattagatga acatcagaca atgcttcaat caatgattga
    12961 tcataacttt gatgaagtcg gatttctttg cacgatgcat ttacatatga ttatcgagga
    13021 gcaagaatac gtgattcata attaccccga ttattttgaa gatattcagg acagacccat
    13081 caaataagag gttttggaat cagcgttttg acctgagtat tagagaaaga aacggcaaaa
    13141 tagttcctca tattttgccg ttttttttga gctaataccg agggtcagct gattgcacac
    13201 cgtttattga gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
    13261 aaatagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    13321 caccgttttc caagaggtcg tgagcctgcc gttcatctcc gagtcacaag gaacaatttc
    13381 aaaaaacagc ttctcatgtt tgttgaatat tttgacttgt gaccgaggag ttgacaggag
    13441 aacatcgttt attcggaaaa aaaggactgt gacacgactt ttgtcacagt ctctttattt
    13501 gtaatctaaa tcatactccg tacatctttt ttctattttt tttatttctt ctaagatccc
    13561 ttggctacga atttcattat atggccaaag taagactgcc tctaatacat aaggcgaaaa
    13621 atcgctttga ttcatctctt ttgaaaaact catcgctgct atctcgctca atgcacgaat
    13681 gtttgattga tgtgtataaa aaggcaaccg ccacagcaca attttttcgc tttgcgtata
    13741 aattccctta acagaagctt cgatgtggtg aaatagttca tgtccaataa tcaaagcttg
    13801 cgctgtttct ttagagatcg aagctagtcc tgaacatttt aatttttcta acggctccgt
    13861 catcaatcga atcccttcat cttctgcata agttgcaaaa gtgattcgtt cgtttatttg
    13921 ctctgattca caggaaacga ttggaacagc catttgttca caaatttctc ctacatcggc
    13981 aaaagggtac ttttttttga tgctacatgc cgtttcgata ccacaatcga ttgattgttg
    14041 tataagaatt ttctgcttgg acaaaggaat ctttttccaa aataaatctc gccgaaactg
    14101 gtagaatccc caaatttctt cggggatctg ttgtaattcg tttagtaact cttctaactt
    14161 atgtttgttt gatatttccg gcaacgacga tcacttcttc ctccggttcc atagtagata
    14221 cttccagatc catcaaaagc tgtagttgtt ccagatccgt cgctgtaaaa tcagagactc
    14281 ttgctcctaa gcagagataa aattctggtc gggcaatcaa ttcagtttga taaactgcca
    14341 tatcttccca aagttgttcg acagctgtca tataattggc tgcagttgtt ttcatacatg
    14401 aagcatgtcc acgttggacc ttgataaatc ctttttgatc gatgatccga agacttccag
    14461 cattttttga acgattctgt tcgccgcata cataaaagaa aggtgtattt tccaataagc
    14521 aaacttcatt tttatttaat cgcatatcct ctgcagcaag ctctagtgct tcttctgttg
    14581 tgatctcctt ggttagatcg gttgctttca cttccgtaga accagtagcg atagccgtta
    14641 cttttgaagt ctgtggatcg atttctacgt ggacttcgat cgattctggt gtagcgcctg
    14701 attcaatcgc tttattcatc gcttcatttt tcaacgatcg tatatcttct ttacttggcg
    14761 aagggatgat acgttctaca acatctcgga ccattgcaag tgctacacca atagaagaaa
    14821 tcacttcggc gttttcagga atcgaatatt tcacacccat tttattacta aagtaagtaa
    14881 tcaatgaggc agcgccgccc cctacaccta caagagaaat ctgatctttc tccaagtgat
    14941 acttgtctgc caattctaaa atcaccggtt caatcttagc ataggatttt tccatgatct
    15001 gttctgcgat gtcttcaacc gtcgtatgac aataatcagc taatgcttgg atggcttttc
    15061 gtgcagatgg gacatttcca taagaaaaat gttcttcttg cactaatcca aggacattgg
    15121 ctgcacatgt atttgtaata gtcacttctt ctccgtcttc catcactacc ttgacgtaat
    15181 cagcagggtc tccttcttta ggagagaaaa attcaacctt aggacctttg attttttctg
    15241 tctccgtaaa tactgcataa tctagaccgg caatatgtgc agaacgtgga ccgacatcga
    15301 tgattcctga ctgattagcc cgtaccatcg aaccgcctgc cacacctaga acccgaacat
    15361 ctaatgaaga gatatatgtt gggtggccac ctacaattga ataatcgatt gctggacgtc
    15421 catttttaat gaccccgata ttggttgttg ttccgcctac ttcaaaataa acaccattag
    15481 acgctctcaa gtacatcaaa gagcccatga ctgatgctgc tggaccggaa agcatggtta
    15541 aaacaggtcg ttttttcatt tcattgattt ccattactcc gccgtctccg cgcataatca
    15601 tcaaagaaac attgacccct gcttctcgta cggaatcttc tgtagaggtt gccgtatcta
    15661 gcatttttgg taaaatagat gcattgattg ctgctgtccg agttcttctt gtcaatccat
    15721 ataattttgt gatgtcagaa gccatcgttg tagggatcga ttttaccgat gctgcttcat
    15781 aaacaactcg ttctggttcc ccattatcta ctccaaatgc catagaagaa accaagacct
    15841 ccgctctttc tctttctaaa gaagaaatcg tttctgaaac acggtctaca tttaagtgtt
    15901 tgacaggtaa gaatgcattg acaatctcta tttttttctt attccctaaa tcgatatcgt
    15961 tcaatctagt ttgtcgtttt gctaaaaaac cttctaatcc accttttgcc atcccgatta
    16021 cgccgacttt tgctacgtct ccttcaatca gtgcattagt cgcttgtgtg gtagaatgtg
    16081 caacaaacac aacatcttct ggactgatat tattttccag caaacaattt tgaaaagact
    16141 gaacgacacc tgcggctact ccacggacat catcgtgggt tgttttcact gaagattttc
    16201 cgatgatttc gtgcgtcgca ttatcgattg cgactgcttt cgtatgtgta ccgccaacat
    16261 caattcccat tcgcacaaaa cgtttcattt tcttttcctc cattcatcta atcgttttta
    16321 tcgacgtttt gtttactagg cgttcccgta caataagaac gtgatcaggc ataagatcgc
    16381 tccgatgatc caaccagttg ggatggattt cttcatatat tctcgagaag ttacttttgt
    16441 ataacctaat ccccaagcaa cccaagattg tgtaatatct aaatgctgag gagcaatcgt
    16501 tgtgattgca aataacggga acaaaaaggc tggcgacatc gttggattaa cagctaatac
    16561 gacagctaaa atagctgagc ctgaaccgac tagattcatt ggaccgcgga aaaatcctag
    16621 tggtgtcaat atagcaaata cgattgccaa gactaatggt gattgcggta tccaatctcc
    16681 aaaaatagct gaaaaataag gagaagcata cgcagctgaa ttattgaaca tcgctaaggt
    16741 catcaagaaa ccaaccattg gcgcaacatc tactgcgcca tctgtaaata atttcgcaaa
    16801 ctgacgacag atttctttat aacctccgcg caattttcca gtaaacagca atgcccagat
    16861 acctgctaga ataaaaccta agatgattgg aagatctaaa agaacgaccc ctaaaactgg
    16921 taataatacg gctaaccatg agatcattgg tgcgtcgcca ccttcagctg gtacgtttgc
    16981 tgccattgca tagcgtttct ttttgttcat tgatatatta gcgaccgtta gtaccacaac
    17041 taagcttaca atcatcccaa tcataccaat ttggaaatag tcattataag tatagctttc
    17101 agccgctgga ttgaaaccag cataaatcgt ttgatattgt ttgaaattga cgatattagc
    17161 aaagattcca gccataatcg agcccataaa tgagaacagt gcaaccggta ctgaaatacc
    17221 taatgacatc aagattggca acacaataac tgcaatagaa atcactggtc cgattcctgt
    17281 catcgacatg aatataacag cggttacaat acatagtaag ctcatcgtga ttcttggctt
    17341 gtcccctcca agttcaacta cctttcgaat cagggtagct gcgattccgc tatctactag
    17401 aacacgcccg aaaaaggcac caaagaagac atttaccaag atcgatttgg cgtactcagc
    17461 tggccccgtc gtatagatat gcgtcaacac atcaattact ccttgccctt ccatcgccgg
    17521 gtttggtgaa aacgtgtttc cgactagcgc taatcccatc catacagtag ccatgatggc
    17581 aaagccgacc atcaagttgt agccttttac acagtaccag accatgaaaa agaatgagat
    17641 aactaacaaa ataccaataa taatatcact catgttattc ctccgtccat tttgtttttc
    17701 tgataagcaa actaatatat cagtcaaact cattctaact tatgtagtta gcgctttcat
    17761 taaagggtga ccataaatgt tcagttttca attaaaaaac cttaattatg ctattttaga
    17821 cttatacgtt attgtcctgt ttcatttgat ggaggattgt aaataaaaaa agaggaaaga
    17881 acataacttt tcgttatctt ctttcctctt tataattgtt tagcttattt agccaaagct
    17941 tcttttgctt ggtcagccaa tgcagtaaat gctgctgcat cgttaacagc caagtcagct
    18001 aacattttac ggttgatgtc gatttcagca actttcaaac cgtgcattaa ttttgagtag
    18061 ctcaagccat tcatacgagc tgctgcgttg atacgagcaa tccacaattt acggaagtcg
    18121 cgtttctttt gacgacgatc gcgatatgcg tagttgtatg aattcattac ttgttctttt
    18181 gctgttttaa ataaagtgtg ttttgaaccg tagtaacctt ttgctaattt tagcactctt
    18241 ttacgacgtt tacgagttac tgttccacct ttaacacgtg ccatgtataa ttcctcctaa
    18301 taaaaataaa tcttcagatt ctgttatttg tacttcttat ttcatttttg ctagttgttg
    18361 gcgaatacgt ttgaagtcgc cactagaaac catgcttgct ttacgcaatt gacggcgttg
    18421 tttttttgtt ttgccgtgga aacggtgact tgtaaacgcg cggaagcgtt tcaatccgcc
    18481 tttaccagta cgtttgaaac gttttgctga tccgcggtgt gttttttgtt ttggcatgac
    18541 tatatttcct cctcaaaatc tttcgttatc cgactatcgg ctgtccaaca gccagttagt
    18601 cattcaaatt acttactgtc gtttttcggt gccagcgtca agaacatgct gcgtccgtcc
    18661 attttcgctt tttgttccac tgttgcaata tccgcagttt cttcagctaa gcgattcaag
    18721 actttttgac caatttcttt atgggtaatg gcacggcctt tgaaacggat agaagctttc
    18781 actttatcgc ccttttccaa gaacttacgt gcattacgaa gttttgtatt gaagtcattc
    18841 aagtcgatcg taggacttaa gcgaacttct tttacattga tcactttttg ttttttacgc
    18901 gcttcgcggt ctttcttttg ttgttcgaaa cggtatttac cgtagtccat gattcgcgca
    18961 actggtggtt tcgcaccagg ggcaactaac acaagatcca agtttgcttg ttctgcaata
    19021 ttcaatgctt ctgcttttgt cttaactcct aattgctcac catctgaacc gatcaatcgt
    19081 aactctcgtg cacgaatgcc gtcgttaacc atcatatcct ttgctatggt cattcacctc
    19141 caaatattat gagagaaaga aaagcttacc gaataaacgg caataaaaaa acgagttctc
    19201 ttacagaacc cgcgcgtact tcacctaata gaaccagtga aaactatcta gcccagtgac
    19261 attatcaact aaggcgagaa gcgggtgctt ctgcttttat ttctcaacca tcatagtata
    19321 gcaaaaacta atctttttgt caatgttttt catacagttt gtcttccttt tctgaaaatg
    19381 attagattag cagactttct agcagtgatt cccgcttctt tgctatacta aagaaaaacg
    19441 aggtgaaccc acatgaaaaa aatcgaacct attccacctt ctcttcctct tcttacaaag
    19501 ggagagtttt atttagaaga tgaggagatc ttctctggtc ttctgatcaa cgggggtgac
    19561 caaagctatc tcgatgccaa aaatatggta ttaagagaga gtcatatcca aaaacttacg
    19621 atgcaaaaaa cagttttacg tcattttgaa tgcagtaatg tcatttttga aaaatgtgat
    19681 ttttccaatc tggagtggat tggtggcagt ttccaccaag tcgtgtttca tcaatgtaaa
    19741 ttaacaggta cgaattttgc cgaaagttat cttcgagact gtactttcac tgattgtatt
    19801 gcttcgatgg cttcctttag tagtacaaac ttgaaagctg tctccttcga tcattgtcaa
    19861 ttagaagaca gcgaatttta tgaggtcact tggaaaaacc tttttttgtc tgacaatcag
    19921 ttgaccggtt cgaactggtt tcgaacttct ctgaagggat tagactttac gactaatttc
    19981 ttcacagcaa tcgctttatc tcaagattct ttggcgggac taatcgtcaa ccaagagcaa
    20041 gcacttgtga tcgcccaagc ccttggtttg cagatcaaag actagatcaa cgaatccgcc
    20101 gccacagtgc aataagcatc caaatagata agaccaaact gatgatcaag gcgattcccc
    20161 atccgaaaac ggaatgggta aagggtaact ggagattcat tccgaagaat cctgtcacaa
    20221 tagttggaac ggtcaagatc agcgaccaga ccgtcaaaaa tttcatcgtg tcattcaagt
    20281 tgttattcag caaattatta taagtgccag agagctggtc taaaatctgc gaagctaagt
    20341 tcgtcatttc aactgcttgt cgtgcttcga tcagcgcatc gtccaactct tctttttcct
    20401 tttctgacaa cttgcgataa atagccaatg ccttgatttg ttctaaaagt actacatttt
    20461 gttttgttcc tgtaactaag taaaccaaac cgatctctaa atctgaaagt tgcagcagat
    20521 tcttgttcgt tgttttttct cttaatttca gattaagccg ctgacgttca ctattcactt
    20581 cctcgattaa aggaaaaaag gaatcagaaa tcaaaaacaa tgtacgaaac aataaactgt
    20641 atacactcat gtctggcatt cgttccaaaa gcgatcgaat tagtcgaatc acgtactcgg
    20701 tattcggcgt ggtaaatgta aacaaaccgt cttcttttaa aataaaagcg ataggactag
    20761 tttcataatg attatcgact ttttgctggt gcggaacatt tgatacaagc agtaacgctt
    20821 catcaaaaga atcgtactct acgcgcgccc gttcattttt atctagagaa taagtcagca
    20881 tctcgtcact aatgccatat tcttctttta agtttttaac agctagcaca tcgttttgct
    20941 gtacattgat ccaaaaatta cggtcttgat taaaatagtg tttttcccgc atactctatc
    21001 ctcctgacat ttgtttccag tataaagaaa aaagtcgctc aaataaaggt ttacccttta
    21061 tttgagcaac tgtaaatcaa atggctactt tggtttggcg aatctctgct aatttttctt
    21121 cgatcatttt caagacttct tcttttgctt gaggatcttc cacaaaatta tagcggtctc
    21181 catcgatttg gatttttgga ctttctttgt attcttcata ccattgatcg taacgtctgt
    21241 tcaattcttt gtaataatcg tatagacttg gatcaaagga caattgttca tacgaacgac
    21301 cacgtttttc gatacgctcc aacatcgtat caaaagatac acgaatatga actaataaat
    21361 ccggatgttt tttatgggca gcatagggaa gttcttccat catgttttcc agcagttcat
    21421 catacacacg aacttctgtt tctgttgctc ttcctagatc tgcatttaga tggaaaagca
    21481 gtgaatcttc ataaatcgaa cggtccaata cattattgtc atctttcatt gcttgtttga
    21541 tgcttgcaaa acgtttattc aaaaaataaa tttgcagcaa aaaagcatac tgttcagggt
    21601 ttgcataaaa taagggcaag acttcattat catcaatcga ttcataaaat gcctgactat
    21661 caaagtgttc agccatcatt tctgttaaac ttgattttcc ggcaccaatt gtacctgcta
    21721 aaacgatcac gctcatttac ctccagctca cttcatcact aaatattgtg tttaactcca
    21781 accttttta
//
LOCUS       DAYYAG010000041        21486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000041 DAYYAG010000000
VERSION     DAYYAG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.041"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHARQ_001320"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHARQ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHARQ_001321"
     CDS             complement(1878..3080)
                     /locus_tag="ACHARQ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8409748.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHARQ_001322"
     CDS             complement(3140..3343)
                     /locus_tag="ACHARQ_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8409749.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHARQ_001323"
     CDS             complement(3485..4552)
                     /locus_tag="ACHARQ_001323"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8409750.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHARQ_001324"
     CDS             complement(4610..4717)
                     /locus_tag="ACHARQ_001324"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409751.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHARQ_001325"
     CDS             4880..5692
                     /locus_tag="ACHARQ_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8409752.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHARQ_001326"
     CDS             complement(6066..6257)
                     /locus_tag="ACHARQ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409753.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACHARQ_001327"
     CDS             complement(6502..6732)
                     /locus_tag="ACHARQ_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409754.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACHARQ_001328"
     CDS             complement(6716..6913)
                     /locus_tag="ACHARQ_001328"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8409755.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACHARQ_001329"
     CDS             complement(7059..7937)
                     /locus_tag="ACHARQ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8409756.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACHARQ_001330"
     CDS             complement(7976..9094)
                     /locus_tag="ACHARQ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8409757.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACHARQ_001331"
     CDS             complement(9091..11190)
                     /locus_tag="ACHARQ_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8409758.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACHARQ_001332"
     CDS             complement(11241..12062)
                     /locus_tag="ACHARQ_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8409759.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACHARQ_001333"
     CDS             complement(12059..12868)
                     /locus_tag="ACHARQ_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8409760.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACHARQ_001334"
     CDS             complement(12889..13383)
                     /locus_tag="ACHARQ_001334"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8409761.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACHARQ_001335"
     CDS             complement(13395..13895)
                     /locus_tag="ACHARQ_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8409762.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACHARQ_001336"
                     /pseudo
     CDS             complement(13960..16656)
                     /locus_tag="ACHARQ_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(17084..18157)
                     /locus_tag="ACHARQ_001337"
     CDS             complement(17084..18157)
                     /locus_tag="ACHARQ_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8409763.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="ACHARQ_001338"
     CDS             complement(18154..18981)
                     /locus_tag="ACHARQ_001338"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409764.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="ACHARQ_001339"
     CDS             complement(18982..19788)
                     /locus_tag="ACHARQ_001339"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409765.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="ACHARQ_001340"
     CDS             complement(19800..20885)
                     /locus_tag="ACHARQ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409766.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="ACHARQ_001341"
     CDS             complement(20902..21444)
                     /locus_tag="ACHARQ_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8409767.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         6539 a         4623 c         3468 g         6856 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctattta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatatag gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgt
//
LOCUS       DAYYAG010000042        20768 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000042 DAYYAG010000000
VERSION     DAYYAG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.042"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(384..1319)
                     /gene="murB"
                     /locus_tag="ACHARQ_001342"
     CDS             complement(384..1319)
                     /gene="murB"
                     /locus_tag="ACHARQ_001342"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8409768.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1392..2312)
                     /locus_tag="ACHARQ_001343"
     CDS             complement(1392..2312)
                     /locus_tag="ACHARQ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8409769.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2425..3177
                     /locus_tag="ACHARQ_001344"
     CDS             2425..3177
                     /locus_tag="ACHARQ_001344"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8409770.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            4041..4574
                     /locus_tag="ACHARQ_001345"
     CDS             4041..4574
                     /locus_tag="ACHARQ_001345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8409771.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(5064..5588)
                     /locus_tag="ACHARQ_001346"
     CDS             complement(5064..5588)
                     /locus_tag="ACHARQ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8409772.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5581..6054)
                     /gene="tsaE"
                     /locus_tag="ACHARQ_001347"
     CDS             complement(5581..6054)
                     /gene="tsaE"
                     /locus_tag="ACHARQ_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8409773.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(6177..7157)
                     /gene="pta"
                     /locus_tag="ACHARQ_001348"
     CDS             complement(6177..7157)
                     /gene="pta"
                     /locus_tag="ACHARQ_001348"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8409774.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7310..7990)
                     /locus_tag="ACHARQ_001349"
     CDS             complement(7310..7990)
                     /locus_tag="ACHARQ_001349"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8409775.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            8116..8985
                     /locus_tag="ACHARQ_001350"
     CDS             8116..8985
                     /locus_tag="ACHARQ_001350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8409776.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            9011..9526
                     /locus_tag="ACHARQ_001351"
     CDS             9011..9526
                     /locus_tag="ACHARQ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8409777.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9573..10136)
                     /locus_tag="ACHARQ_001352"
     CDS             complement(9573..10136)
                     /locus_tag="ACHARQ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8409778.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(10191..10370)
                     /locus_tag="ACHARQ_001353"
     CDS             complement(10191..10370)
                     /locus_tag="ACHARQ_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409779.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10447..10956)
                     /locus_tag="ACHARQ_001354"
     CDS             complement(10447..10956)
                     /locus_tag="ACHARQ_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409780.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(11052..11432)
                     /locus_tag="ACHARQ_001355"
     CDS             complement(11052..11432)
                     /locus_tag="ACHARQ_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8409781.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11762..12511
                     /gene="pnuC"
                     /locus_tag="ACHARQ_001356"
     CDS             11762..12511
                     /gene="pnuC"
                     /locus_tag="ACHARQ_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8409782.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12695..13972)
                     /locus_tag="ACHARQ_001357"
     CDS             complement(12695..13972)
                     /locus_tag="ACHARQ_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409783.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(14051..14695)
                     /gene="pcp"
                     /locus_tag="ACHARQ_001358"
     CDS             complement(14051..14695)
                     /gene="pcp"
                     /locus_tag="ACHARQ_001358"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8409784.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14894..15838)
                     /locus_tag="ACHARQ_001359"
     CDS             complement(14894..15838)
                     /locus_tag="ACHARQ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8409785.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15840..16526)
                     /locus_tag="ACHARQ_001360"
     CDS             complement(15840..16526)
                     /locus_tag="ACHARQ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8409786.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17000..17320
                     /locus_tag="ACHARQ_001361"
     CDS             17000..17320
                     /locus_tag="ACHARQ_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8409787.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17474..18379
                     /locus_tag="ACHARQ_001362"
     CDS             17474..18379
                     /locus_tag="ACHARQ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409788.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18376..19149
                     /locus_tag="ACHARQ_001363"
     CDS             18376..19149
                     /locus_tag="ACHARQ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8409789.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(19330..20754)
                     /locus_tag="ACHARQ_001364"
     CDS             complement(19330..20754)
                     /locus_tag="ACHARQ_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8409790.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         6096 a         4255 c         3674 g         6743 t
ORIGIN      
        1 acataattct ttttctcaaa ttctaaatat ctagctagca gcagtttttc tctatcttcc
       61 tcttctataa ggtaatccaa cttcatatcc tacacctcaa aaaatgcctc ataccaatta
      121 cttgatatgc agatgaaagt tcatcttttc acctatactg attttatgat ggattatgct
      181 tgttttttca tcagttattc tcatttttta tctttttttt gagaatattg caaaaaatgg
      241 aaaaatttat gaggaaatat tctatatttt ttgttttatt gtgttaaaat gtacaagatt
      301 tgcgccattt gcgtaaacaa ccacaaaaaa gagcaggacg gccaccgtcc tgctctttta
      361 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
      421 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      481 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      541 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      601 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      661 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      721 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      781 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      841 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      901 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      961 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
     1021 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
     1081 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
     1141 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
     1201 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
     1261 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
     1321 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1381 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1441 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1501 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1561 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1621 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1681 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1741 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1801 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1861 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1921 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1981 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     2041 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     2101 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     2161 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     2221 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     2281 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     2341 tttcatctta caccattttt ttctaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2401 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2461 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2521 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2581 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2641 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2701 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2761 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2821 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2881 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2941 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     3001 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     3061 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     3121 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     3181 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     3241 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     3301 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3361 catatttttc gtttggttgg gcttattacc gcagatacaa gcttttgaac accgtttatt
     3421 aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac aaaaatagtt
     3481 cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     3541 attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca aacaaaaata
     3601 gttcctcata tttttcgttt ggttggactt attaccgcag atgcaagctt ttgaacaccg
     3661 tttattgaga agttgtgaaa tcagcgtcct gacctgagta ttagagaaac aaatggcaaa
     3721 atagttcctc atattttgct attttttgag ctaataccga gggtcagctg attgaacacc
     3781 gtttagtgga gaagttgtga aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa
     3841 aatggttcct cacaactttg cataaatttg acttaatacc gagaagtcat gatccccatc
     3901 taactccaaa tcacaaggaa tgagttcaaa aactagcttt tctcaacaaa tattacataa
     3961 aaaattctat gttttttgtt aaatttattt gcttttttca aaagtatgtt acagtaattt
     4021 cccaaggagg ttttacagaa atgaattttg ttgccatgga ttttgaaact gccaatcatc
     4081 agccttatag tgcttgttct cttgcgctag tcatggtaaa aaatagccag atcgttgatg
     4141 aattctatac attgatacag cctgagactc cttttttctg gcgaaatgtc caaattcacg
     4201 gaatccatca agaagacgta cgcaatgcgc cgaaattccc agaagtctgg caaactatcc
     4261 aaccatattt tcaaaaaaat cgtttagtcg ttgcacataa tgctgctttt gatacaaagg
     4321 tcttagcggg atgcttagat tattatcatc ttgaacagcc taattatctt tctttatgta
     4381 cagtgaaaag tagccgtcgc ctgtttcctg aatttccaaa tcatcgattg aacactgtgt
     4441 gcgaaaattt gaacatccaa ttagatcatc accatgatgc attggaagat agtcgtgcat
     4501 gtgcggaaat cctgctttat caagaaaaaa aattcgggac agatccattg aaaaaacttg
     4561 tgactataaa gtaaaagaat acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc
     4621 actccgagtc acaaggagca atttcaaaaa atagcttctc atgtttatta aatattgtga
     4681 ctggtgaccg aggagctggc aggagaacac cgtttattgg agaggttgtg aaaaagctgt
     4741 tctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt
     4801 ggacttatta ccgcagatgc aagcttttgg acaccgttta ccaagaggtt gtgaaatcag
     4861 cgttttgacc tgagtattag agaaacaaac ggcaaaatag ttcctcatat tttgccgttt
     4921 tttgagctaa taccgaaggt cagctgattg aacaccgttt attcaaaaaa aatggactgc
     4981 gacacgactt ttgtcacagt cccttcgtgt gtttttctgc cttctcaaaa ggatgacagt
     5041 ttctcactct tttcctattt cttctattca atcaaataac tcattttata tacatctaaa
     5101 aattcaccta gatctgttct tgcccctcta ggcatgaccg cttcgatttg gaagcccatt
     5161 tttcggtaaa ggtgtacggc tctttcatta cgaacttgaa cgtcaagctc caatcgaaaa
     5221 agtacaccca tttccttcgc ccaactgatg atttcttcta gcataagagt gcctagtccc
     5281 attccccaat attcctgcag aatactgata ccgacttctc ccacgtgaga caaacgaaat
     5341 tgactgtctg ccttgacaga tgctgttcca ataatcgtcc cttcatagat tgcaagcagc
     5401 aataaattat tattgctttc gtatatataa tccaactctt cttctaaggt ttctggtggt
     5461 aacaggagtt ctgcttcatc caaaactaaa aattctgtct ccttattaac ttgtcccatc
     5521 atcaaaagaa tgtcttttgc atctcctggc actgcttccc gcaagatgat cccaacttct
     5581 tcactcatga ttttcagccc atttttctag aattcgctgt ttgaaggttt cactttgctc
     5641 cccgtatgag attagtttca cataccgctt ttctaaatct gtatcacttt tttctaaaat
     5701 cagttccaaa taatcctccg gcagtgcttc ttctaataga tttccccatt caacaacgga
     5761 aagcccatca ccttcaaaat attcatctaa ccctaaatct gctccgctag ctgctacccg
     5821 ataaatatcc atatgataca acggaatccg accttgacta tattctctaa taatcgtgta
     5881 tgtcggactt ttgatcattt gcgttatccc taatccttgg gcaattcctt tcgtcaacgt
     5941 tgttttaccc gcacctagat caccagtcaa tatcagattg tcacctggca ttgcggaatg
     6001 accaattgcc tctgcgaatt gttctgtcat tgttaatcct gatagttcga tcatttttca
     6061 tcttctcccg ctcttctttc tttttgccag tatatcgcgt aatgaagttt ttttaaagaa
     6121 aaaatgaact agtcaagaac agcagctgct gtttttggct agttcgcgtg tatttgttaa
     6181 tcaatcaatg tttgtgctgc tgtgataatc gataatttat atacatcttc ttcatttgct
     6241 ccacgagaca aatcagaaac cggtttgttc aagccttgta gaattggtcc gattgcttca
     6301 aagttaccga aacgctgagc aattttatag ccgatatttc ctgactgcaa ttctggaaat
     6361 acaaagactg ttgcttgacc agctacttct gaatctggcg ctttcagttg tgcaactgaa
     6421 ggaacataag aagcatcaaa ttgcaattcg ccgtcgatca tgtattgtgg cgctagtttt
     6481 ttagcgattt tagttgcttc aactactttg tctacttctg gcgatttcgc tgagcctttt
     6541 gtcgagaagc tcatcattgc tacttttggt tcgatatcga acaattctgc tgtttttgca
     6601 gaatctacgg cgatttcagc caattcttgc gcagttggat tcacgttgat cgcacaatcc
     6661 gaaaagacat atttttcttg atctcttcca cgtaccatga tcatcgctcc gcttgtccga
     6721 ctgatgccag gctttgtttt gatgatctga agtgctgggc gaacagtgtc gccagtcgaa
     6781 tggatcgctc cagagaccat tccgtctgca atccccatat acgtaagcat cgtaccaaag
     6841 taattcacat cttttaaaat cttttctgct tgttctttgg atgcctttcc atttctacgt
     6901 tcaacaaaag tatccaccat ttcatcccac ttatcgtaat tatcagggct gatgatcgta
     6961 aagctagatg ttttgattcc tctggcatgc gctgcatttc ttatttcttc ttcgttccct
     7021 actaaaatgg gttccacaag ttcttctgct ttcaaccggg cagctgcgcc taaaatacga
     7081 gcatcagtag cttccgggaa aacgattttg atcccacggc gaacaatttt aaatcttaaa
     7141 ctatcaaaca attccacaag atgacctcca gacgttttta tatgtattat catacactat
     7201 tccagaaaaa aataacagtt tattttatct gtatcagtca aaaatgctgg aaaaattaag
     7261 tttttcacat acaaagcgaa ctgcactata acaggaaagc gctgttttat tacactgttt
     7321 caggcagttg ccaatcaatc ggttcttctc ctaacgcaag gagcgcatca ttcgttttcg
     7381 agaagggttt ggaaccaaaa aagccacgac ttgcagataa cggactggga tgtggagatg
     7441 ttatgatgat atggcgactc ttatcgatca ttttgatttt ttcttgtgcc ggttttcccc
     7501 acaagataaa cacgattggt ttttcccgtt catttaattt ttcaatgatc gtgtctgtta
     7561 atctttccca acctttcccg cgatgagaat acgcttgccc ttcccgaacg gtaagaacgg
     7621 tgttgagtaa aaggactccc tgtttagccc atgacactag atttccatgt tgaacaggtg
     7681 cgattccaag atcatcataa agctctttat agatatttct taacgatggc gggatcttta
     7741 cgccaggttg cacagaaaaa gataagccat gagcttgatt ttctccatga taaggatctt
     7801 gtcctaaaat caccacttta acttcttcat aaggagtcaa ttccaatgct tcatagatat
     7861 gatacatatc tggatggatc ttttgggacg catattcttt ttttaaaaat tctcttagat
     7921 gcagataata atcttttgaa aattctgagg acaaaacttc ttgccaactg ttatgaatga
     7981 tttctttcaa aaactccacc tcctaataaa aagaataact cacattttct tcaatcacaa
     8041 gggacaatct tcaccctcag cacttgatta atatggtaaa ctaggaacaa gaagcagaaa
     8101 ggaacgataa tcattatgat caaattaatt gcatcagata tggacggcac attattagat
     8161 gcccatatga gtgtttctcc tgaaaatgcc gaggcgatac gttttgcaaa tgatgccggt
     8221 gttgagttca tggtcgctac tggtcgaaac tatcaagaag ctcgcgcagc tttagatgag
     8281 gtaggaatcg attgtgcgat gatcactttg aacggggcac aggtatttga taaagaagga
     8341 aattcattgt ttaccgtgtc gattccaaat aatcaagcaa tctctgtttt agatattcta
     8401 gatgctaacg gtatttacta tgaagtagca accaacgatg gtctttattc tgaaaatcaa
     8461 gcgaaacgaa ttgaaagttt tgcttccatg gtggctaccc atttacctca ccttacgtat
     8521 aaaatggcga ttgccatggc ctcagctaag ctggagttat tgcacatcac atacgtggac
     8581 agtattcgtg ctattttgga agaaaagaat ctggaagttt taaaaattat ttgttttcat
     8641 acagaagggc cagctgtatt aggtccagtt ggaaagcaga tttctctttt aggcgatcta
     8701 gccgttactt cctctggtca aaacaatatc gaagtgaacc ataaaaacgc tcaaaaagga
     8761 attgctgttg cccatgtcgc tcacgagcgt gggatttctt tagaagaagt catgacgatt
     8821 ggcgataact tcaatgatgt cagcatgctg caagtagcag gagtcagttt cgcaatggga
     8881 aatgcagaaa tcgaagtaaa agattatgcc aaatatacaa cggacacaaa ccttgaatct
     8941 ggcgtaggaa aagccatatt gcgcgcgatt gcagaagact tgtagtttta tctagaaaag
     9001 agggatatag atgtcagaat ttttcattca ggaaaagcag cttggacgtg caactcggac
     9061 aattgtcaaa aacgaaaaag gacattccct gtttttgatg gttggtcgct ggggaacaaa
     9121 gggcgatgca ttgtctcttt atgccatgaa tggcgatctc gttgctcaca tcaagcagat
     9181 aagcctgaca ttcggtacgc gttttgagct gtataaagag ttcgaaaagg tgggaaccat
     9241 gcagaaaata ttcaattggc ccggtgactt ttattatatt cgtagacttc actggacagt
     9301 ccaaggtgat atctataatc atcaatatca aatccatcat ttcaatcagc aaatcatgaa
     9361 aatggataaa gcaacacttt tttcaggaga ttactatgta ttggatgtct caaatgatga
     9421 agatgctcca gtttgtattt gtattgctgc ggttttagat tactggctct accgtaaaga
     9481 caaaagtcgc gataagcctg tttcttaccg acttaactta agttaaaaag atgtacctga
     9541 aatgcggaaa tacgcatttc aggtacatct ttttagatat tcttttgcat atgatcataa
     9601 agatgaccac tgatcatccg ctgaccgaca actttgaagt catggcgttc gtataatttt
     9661 ttagcctttg gatttttttc atccacactt agaccgatga cggagcgatt agctttcttc
     9721 gccaatttag gtaatgcttc taataatcgc gaaccgatgc cttgtcctct aaaatcttct
     9781 gatacactga tagagtccaa ataccattcg ttgggaaacg tttctttatc aatgaacaaa
     9841 cgtgtagacg catctagctg atatttctct aagataggta ccagtggctg atcgatgatt
     9901 gattcttctt catctgtata accgtaagca actcctgcaa ctttaccgtc tatttcttct
     9961 acgatacctc gtgaataact gtaacgataa tttggatcag tgattgcttc ttctaaaatc
    10021 tctaaagtct tttgttcccc tgtctgcttc aaaaatggca gttccatatc ttttagaatc
    10081 actaagatca atggggcgat ttctggagca tcttcttttc tcgcaaaacg aatcatagtt
    10141 tcctctcctt ttctccattc taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa
    10201 aaatcttcaa tcttttcagt aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt
    10261 cggcaataag ggcagacgac tggcatttct ttatcattat gttcgactac attattgact
    10321 tcttcgtatg caaatttttt tccgcaatta gggcatttca ttttttccat gttatctcaa
    10381 cttcctttca tctttactct cttagcttac tcttatattg taaaaaagag aagcaatatc
    10441 cttcttctat gcagatatcc atacctgacg gtcctgttct ccatctatta atcggacttt
    10501 ataataagga cgttcatctt tgactagaag tgtccactct ttcacttttg cttttttccc
    10561 gtagtagctg actgcttcct tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc
    10621 tatatcaaaa agatgttctg cggccttttc cgtgtcattt cgtacggttg ttttcccagt
    10681 ggatggattg actgctatga cttgtccttc acaaaaaaac tggtattcat atgttgcatt
    10741 cgtaggattc tccaaatctt tttcaactgc aaaatgaata gcagatatgt tatgttcttt
    10801 tgtttcgttt tttacgattg ctgctgcttt tttcaatgag atccgatatt cctggtcact
    10861 aatatcatcc atgtgaaaag cggcattgag cagtcccgtc ataccggtaa tccctattac
    10921 gatgagggca gcaacaccaa acatcatttt tttcattctt cattctcctg tttctttttt
    10981 ttaaagaata tcaagaaatg aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa
    11041 ttttgttatc tttattcgtg cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc
    11101 attttttttc ctgtaaaggt atcttctcta atcggttctg attcgatccc taaggactgc
    11161 aattctgcaa ttgtttcttc aatatcgtct actttgaaag caagatgacg caagccacaa
    11221 gcttctggat agtttggtcg ttgtggcgca ttagcaattc caaaaatctc caattcagca
    11281 cttcctagtt tcagatctaa tttatagtca tctcgttctt tgcggtaatt ttctctgatt
    11341 acttcaaatc ctaatttatt aacataaaaa tcacgtgatt tctgataatc tgaaacaatg
    11401 atggctatat gatggatcgt tgtaaggttc atatgagcac tcctattctt cgtttaagct
    11461 cggatctata ttaatctcca gttccattgt aaatgtgtca gattgaaaaa aatagctgtt
    11521 tttccttttt ataagaaaaa aggcaaaaaa aacagaaagt aggtcctcca agacactctt
    11581 cctgttccca atataataaa tgaaagtaag aaattatatt ttcaagaaaa caaacagaaa
    11641 attatcataa cacaaattat ttataattga aagactagtt ttttttcaag aaaagcatac
    11701 aatagattgt gttagtaaat acagaacaca ctacatttag tgtcttaagg agtcctcata
    11761 catgaagtca tattttcagt ttttagctca tcagctaaaa ggctggccgc aacaaaatta
    11821 ttatcttttc tttttcagtc ttggctgcca ggtcatgact cttgtcaata gtcccatcac
    11881 ttggctgtcc gttattacat ttatcggtac cacgcttggt gtattgtgta ttttatccat
    11941 caatgcggca aaatcagtca acggtatttt aggtattttg tctgcgattt gttttattat
    12001 cgttgggttt tcagcaaaga actatttaag tatcggtgaa cagattgctt atatgatcac
    12061 attggatatc cccgtattgt taagtaaaga atggaatcat aatatggctt caaaaatccg
    12121 aaaattcact ggaaaaagct ggtttatcgc aatcatcagt actttccttg tctatcttgt
    12181 ttcaggattc ctgattggtc gttatacaga tgatcctcgg ccgtgggtag atgccattgc
    12241 tttttctatt agcttgactg ctggtatcat cagtttcttg cgttacaaca accaatatta
    12301 ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg tatatttcat ttacgcatgg
    12361 atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac ttgctgaatg atgtccttgc
    12421 atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa cgtatgcaaa aaatggaaga
    12481 agaatactac aacgcacaac aacacgcata aaaaaactga gcgtgggaca aaagtaaaga
    12541 acacttttgt ctcacgctct aaacacgtat aaacggcgga atcagaagca actccttcgg
    12601 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct
    12661 tggagttaaa cgcttctgtc ccagcctcta ttttttatag taattccttc agatactttc
    12721 ttgcctcatc attgaaacgc agtgattctt ctaacttctg ataatattcc aacgcttgtt
    12781 tctgagcttc agtaaaacga taatgttttc ctttccattt gtcgattgtc tcttgcacat
    12841 ttccagattg gatatctttc actactagtt gccatttttc ttttgagact gtacggtctt
    12901 tcttatcagc agattgtata gcttctcgtt ctttacgcaa tacttttaaa aaaccatttc
    12961 tgactatccg aggaatgctg tcgaagaacc cttctgtaaa accatagcga caatattgct
    13021 tccaaataaa tagatagatt tttggttgga tgaatcgttg gtttttcgca aaatattcca
    13081 tctgagcagt gacttctggt gttcgttgac gttttgtctt tctcaacacg gaaataaatg
    13141 tttcaaaata agcattagga tgtactggca cttcttcata tacgatggat tgccaatctt
    13201 tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac atcctttact aattcttctg
    13261 caaagtcgtc aaatagttct ttttttatct gtcgcttttt tgactgccca ttgatgtgtt
    13321 cacttatttt cttgatttca ttttccaatg cttgataatc cgctatgtaa atcggcattt
    13381 ctacttggcg aaattctgcg agccgctgag ctaattttat atccggtaaa tatggctgtt
    13441 tcatccgacg attttggtac aagacaaaac agtatttttg ccatagtcct tcaggaaaat
    13501 cgatattctt ctgcttaagc cataataatt cgtctaaggc cagatcaact tttgtcattc
    13561 cctgatggat cgaagctgcc actgtcggag agacatgcag gtgatcggca aagtggttga
    13621 tgcctagttt taggatcttt ccggtttttg gcgatttaac gatgtattgc acttttaatt
    13681 ctctaccaca ctcacaatac aatttttgct ggcttcgttt tgttcgaaaa aacggattga
    13741 tcttgaagtc agaaaacacc caatctgttg cagctaaata atgttcttcc ataaaacgag
    13801 agctgattag atatcggcgg tgcttatcga ctagttcccg ctgttttttc gttaattttg
    13861 catagatctc ccaacgttga ctgcttgtgt attgtttaga aattttgatg ggatagcttt
    13921 tgctgatttc ttttggaaac cattcttcaa agacggattg ccattcttcc acggctgttt
    13981 ctttcctttc taattacaaa aagctggcta ctaacataac gttagcaacc agccagtgtt
    14041 tcttgctaaa ttagtgagtt gttccggcat ttccggaaat atcttcttta ttttctactg
    14101 ctgcttcaac tgcataagtc aaagaagaag caatgtcttc taaagacata gaaggcgtac
    14161 ccactggacg atcgatcact tgatcgggtg agaatggcac gtggatgaac ccacctcgaa
    14221 ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata catgatatca ttgcagacaa
    14281 atgttcctgc tgtataagag atgtacgctg gtagaccatg atcttgtaca ttttttgtca
    14341 ttgctttgat tggcaatgag gtaaagtaag cggctggacc gttttcttct aaagccgtat
    14401 cgaatggctg atttccttca ttatcaggga ttcttgcttc tgctagattg attgcgacac
    14461 gttcgaacga gactgctgac cgcccacctg cttgtccgac acagatcacg acatctggtt
    14521 ggtgctgttg gatggcttct ttgactactt gactgctttt ttcgaatacg gtcggtactt
    14581 ccactttgat gatttcagca ccgctgattt catctggcat tttttttact gcttcatagg
    14641 ctggattcac tttgtcccca ccaaatggat caaaacctgt tactagtact ttcatctgtt
    14701 gttcctccgt ccgattgatt taacatatgg ttatctttta tcataccatt ttccttgaac
    14761 agcgtaaata aaggaaacaa tagtttaaat aacataacag atcaaactta atatattctt
    14821 ataaaaaaat agtgttccac tacaatgtta gaagaggttg cgacaattat tctgtcacaa
    14881 cccctctctt ctattattta aatagaatca tataagcaat ttggaaaact agcataaata
    14941 acgcaataaa caactggttt ttgatcactc cgtatttgtc tttcatctcc agcatcgcta
    15001 caggcacgat attgaagtta gcagccatcg gtgtcagcag ggtcccacaa aaaccgcaag
    15061 tcaatgcaac cattcctatt aacgctggat tcgctccatg actaaatacg aatggtgcac
    15121 cgatcccaac cgtcatcacg gtgatggccg caaatgcatt ccccatgatt accgtaaata
    15181 tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac atttccttct ggaacgatcg
    15241 tacctactgc attggaaatc actgtcccaa ctccagcgct tgtaaatacc gcacctagag
    15301 aagctaaaag cattggcaac atgcttaacg gccctactgt gcccagcata tctgctgcat
    15361 catctaaaaa tgtacttggt cgattatctc gagaaaagaa catcaagatt agcatggctg
    15421 caaaaacacc gacgcccacg ccgactaaag cacctaaatt ggtaaataga gcaaacaaga
    15481 tcgcaaagac accgatactc aaagctggaa taaaaatttt cattcctagt ttgtctgaca
    15541 ttttttccat atattctttt gatggaagcc ggctttctcc tttgctgact ttcttagcaa
    15601 ttgctggtat ggtcatcgca aagatcaagc caccgttcac gattgccgga agccatcttc
    15661 ctcctgcaat aactacccct aagaccgtcc agaaaaaggc tgacccataa cgatgaggat
    15721 tggttttatc tgttaaattt cttactccag tgtaaatcaa tacgagtccc atcacgatat
    15781 aaaggatctc cagtaattta tcgcctaata aaatatcagg atttgtaaaa aagtccatct
    15841 tattgcccct ttcctggata atatttcttc gctaatttct tttctttaag atagaagtag
    15901 acacttgcca caactaaagc cgtgatagcc actggaactt ccactaaagc aagctgtccc
    15961 aatgttacgt catatcctag atctttcagt gttgattgga ctagcaacgc ccctgctcct
    16021 ccgatgaata gtacttggcc aaagaaccag cagacatttt ccatagctga agccatacct
    16081 tttagttctt cttcatgctt aggatggact tctaaccctt ttgattcgat ggtccccaat
    16141 gccatcggta aaatgattgg acgaacaaat ccagcaactc caccaaaact cacgttaaaa
    16201 gccgcaaaga tcattcgaaa gatcccataa atatctacaa tcataccagc agatactttc
    16261 ttgaatcgct tgatcaagga agccgctgat tctttcagac catttctttc aagcgttcct
    16321 gttgccaaca taatgatgat aaaaatcgcc atcccgcgat tgtttacaaa acttgtacct
    16381 aaaagttcta acattccttg aatgccaaga ccgccagtca aagctgtaac aactaaagca
    16441 accattacga tcaatatcga gtctaattta aatgcaaacc cgagaataac aatcaagacg
    16501 cctaaaaggc taagataatt ttccatatat tttcctccat aaaatgacaa tattttttta
    16561 attataaagc aaaacacata aaaaatatag ttgttttctc acgaaataga cactattttt
    16621 aaatattttt cgttattttt ttatcacaaa aattatctta cttattgtaa ggtaaagctt
    16681 tttttaattt attgcccgat aatatataga aataaggtct agttactgtt ttttttaaat
    16741 gattaataaa taacaacttc tttctatgaa attgcaaagc attcatcttc ttttccgcta
    16801 acgatatatc cgtcagtgct tggtacactt tactaaaaag tatgagattg ccgatatctg
    16861 gataggtgtg ctatactaca gaaaaagata tacaatctgc caccgggtag gataaagagg
    16921 atgatcattc cattaggtct ttgaagggat gattttcctc tttttgtgta tttttttata
    16981 gttaaggagg aatgtaagta tggcatggat ggaattaatc atagcaggaa ttttggaagt
    17041 attctggtca acgatgatga aatggagtga tggtttttct aaaatcaatt attcttttta
    17101 cacagtgatt ggcatgattg ctagttttta ctttttgtca aaggcaatca aaagtttgcc
    17161 gatgagtctc tcttatccta tctggacagg gattggcgca gttggttcgg tcataattgg
    17221 tgtggtcttg tttcatgata agctgaatct ttccacttgg ttctttgtgg ggatgctact
    17281 catcagtatc attggcatca agattactag cggccactag acacttggaa taagtactga
    17341 tcagaaaaaa taagcctttt tatttaatat tcaatttttt tatgaaaaaa taagaaataa
    17401 gtccaatgac ctagcgactt ctccactcat caggtgtatg cttgtactat ctatagaaaa
    17461 ggagtgaata tttttgaaaa gtctttcggt ccaagaatta tccaagaact ttggtcacaa
    17521 acaagccctc agcgatgtca gttttagctt atcccctggc aaaatcgttg gattagtagg
    17581 tcctaatggc gctggaaaaa caacgatcat gaaagctatt ttaggactga tccattactc
    17641 agatggaaca atctgtattg gtgaaaatga gatttctccc tctagtcata aagggttgga
    17701 acaagtagga gcattgatcg aatacccggg tatctatcca ttcttaacag gctatgatca
    17761 tctgaagtta ttctctgaaa ccaacgatgt atctgctatc gacactatcg tgaatcagct
    17821 caagatggaa aagtacattc acaaaaaagc gaaatcctac tcgttgggaa tgaaacaaaa
    17881 attgggaatt gctcttgcct tactgaacca tccgtctatc gttattttag atgagccgat
    17941 gaacggtttg gatccacaag caacaagaga tgtgcgaaaa atcattaccg acttggctac
    18001 tcagggcacc tctttcttga tctccagtca cttactcagc gagcttgaaa aaatggtcga
    18061 tgagctgatc ctgatcaatc aaggaaagat catcaaacaa tgtacaatgg ctgaattgaa
    18121 ccaaacagcg caagatttca tcgttatctc tacaacaaat gatctagctg ctcgaacgat
    18181 cttgacagaa gctggatata ctttggaaga tcctgataaa atcaagcttt taaaagaaaa
    18241 cgaagaatta ttaggaaccg tgatccagct attgtctgca caaaatatcc atgtgacaga
    18301 cgtccagcat ctgacgaatg atttggaatc ttctctattg gaaattctgg attcagtaaa
    18361 gggggaagaa gaaaaatgac attgcttgta aaacaagaat tatttaaatt gatcaagaaa
    18421 aagtcaactg ctgttttatc tgttctatta gttgttttgc tcataggaac agcacttcta
    18481 gccaaaaaat acacgacgat cattgatcca gttgaaatga ctgctcagct ttttagtgcc
    18541 acatcttgga ttgtctttat catgattgca gctgcaagca cgatcatttc gatggaagca
    18601 caatatggta cattgaaaaa tctcctttat cgaaagtatt ctcgaggaga aatattagta
    18661 agcaaatgga tcacattagt tatttattct gtttatttat accttttagc tattattgtc
    18721 actgtgttga tgaaactgat tcttttccct tcgatttcct tcacggagaa ggtttctact
    18781 ggtcaaacac tgatccaaag cttgttcact tatactttgg gtagttatat cggtttatgg
    18841 ctgattttaa gtttagtgtt gatgattgct tgctttatca atagttccgg cgcatccatc
    18901 tcagcaggaa tcgtctttta ttttgcttcg tcaatcatat ctggtatcct tattgcactc
    18961 atccaaaaat gggagtggat caaatggaat ccgatcagta tgttgaatct gcaaaaccaa
    19021 attggaaacg aagagatcat gaagcaattg actcatcttt ctactaatca aatgctattc
    19081 ggtaatttag cttacatcgt tttgttctta gcattaggat atctcgtatt taagcgaaaa
    19141 aatgtatgat gactcatcaa aaaagaggct gtgaaagaga cgttcagcct tattgccgat
    19201 tggctgtctc ttaaacaccg tttattctga aaaaggagct gtgacaacat ttgtgtcaca
    19261 gctcctttcc tagtatgttt ttgaataaac gacaggctca caacctctct ttatacttct
    19321 tttatacttc tatacgtgaa gcatcccagt ggcaaataaa accattgcaa tcactccgac
    19381 catcgtcaca cctataagta aaatcttatc taagtggata tgttgtacag gctcgttttg
    19441 ctccttacgt gctttgaggt aaaaataaat gcctggcaag aaaagaacaa gaacaagcat
    19501 tacttcatta aaggctacga ctagcatagc ccatacttga aaggcacttg ctaaaagccc
    19561 tactaaaaat tggacccatt ctttccgctg ataagaaagt ttcatttggt acaatcctaa
    19621 gaaaagccac gtgaagaaaa tagctgctgt accaagtgtg taagcaaagt tataagcact
    19681 gtcagtgaat aataatgaaa gtaaaaagac attcgtacat aacgtcgtca atacgatagc
    19741 ataagttggt gccccatttt tgttgagttt tccccatgct ttaggcaaaa gtccgtcttc
    19801 agccatgttt cccattgttt caatcggtaa gatcgtccat gagagccaaa caccaactaa
    19861 agaaatgatc aaaccgccgt tgatcacgac tgctccccat ttaccaacca tcatttcaaa
    19921 cacatagccc atagcaggct gcgccttgat cgtttgcagc tgattcatcg tcattgtccc
    19981 atatggaagc atggaaacca agacgtaaat aataatcaag ccgaatatcc ctagaatcgt
    20041 tgctttccca acatctgatt gtttttttgc tcgtgaacct acgacagagg ctccttcaat
    20101 accaacaaat acccaaagca tcaccatgat gcaactcaat acttgactgg aaaacgggat
    20161 cggctgtcca ttcgcattca atataatgtc gctagaaaag ttatcaatga attgttggat
    20221 cgaaaacgtg ctgatagcta ctacaataaa taaaaataat ggcactaatt tacagaatgt
    20281 gacgatagca ttgacgaaac tagcattttc gactccccga ttcactagcc atgcatatcc
    20341 ccataaaaag actgtactca tcacgatcga tgccagattt tgcccacctt caaatactgg
    20401 gaagaaatat cctaatgcgc tcatcaacaa tgttgcaaaa gctacgttgc ctaaccagca
    20461 tgtgagccag taaccccaac cgctgataaa accagcgaaa cggccaaaac cttcttctgc
    20521 gtaactaaag atcccgcctt ttaaatctgg acgtttttta ttgatattat tgatcgacaa
    20581 ggagagcatc aacattgctg ttcctacgag tacccaactg aaaatagctg ggccgccagc
    20641 cgatccggag atgtccgtca taataccaaa tacgccacct ccaatcgctg aaccaattac
    20701 taaggcagca agtggaatta aaccaattcc ttttttctgt tgctctgtat ccatgtaagc
    20761 atcctcca
//
LOCUS       DAYYAG010000043        19974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000043 DAYYAG010000000
VERSION     DAYYAG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.043"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACHARQ_001365"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACHARQ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACHARQ_001366"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACHARQ_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACHARQ_001367"
     CDS             complement(1147..1716)
                     /locus_tag="ACHARQ_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8409791.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="ACHARQ_001368"
     CDS             complement(1790..3079)
                     /locus_tag="ACHARQ_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409792.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="ACHARQ_001369"
     CDS             3406..4281
                     /locus_tag="ACHARQ_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8409793.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="ACHARQ_001370"
     CDS             4295..4618
                     /locus_tag="ACHARQ_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409794.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="ACHARQ_001371"
     CDS             4615..5367
                     /locus_tag="ACHARQ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8409795.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="ACHARQ_001372"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="ACHARQ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACHARQ_001373"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACHARQ_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8409796.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="ACHARQ_001374"
     CDS             complement(9831..12017)
                     /locus_tag="ACHARQ_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8409797.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACHARQ_001375"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACHARQ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8409798.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="ACHARQ_001376"
     CDS             complement(12239..12679)
                     /locus_tag="ACHARQ_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8409799.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13251)
                     /locus_tag="ACHARQ_001377"
                     /pseudo
     CDS             complement(12753..13251)
                     /locus_tag="ACHARQ_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(13536..15002)
                     /locus_tag="ACHARQ_001378"
     CDS             complement(13536..15002)
                     /locus_tag="ACHARQ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8409800.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15313..17394)
                     /locus_tag="ACHARQ_001379"
     CDS             complement(15313..17394)
                     /locus_tag="ACHARQ_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8409801.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17536..17658)
                     /locus_tag="ACHARQ_001380"
     CDS             complement(17536..17658)
                     /locus_tag="ACHARQ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409802.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            complement(17918..18277)
                     /locus_tag="ACHARQ_001381"
     CDS             complement(17918..18277)
                     /locus_tag="ACHARQ_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8409803.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18307..18648)
                     /locus_tag="ACHARQ_001382"
     CDS             complement(18307..18648)
                     /locus_tag="ACHARQ_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409804.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(18645..19319)
                     /locus_tag="ACHARQ_001383"
     CDS             complement(18645..19319)
                     /locus_tag="ACHARQ_001383"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8409805.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
BASE COUNT         5684 a         4211 c         3454 g         6625 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtagctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttcctttttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttt ggttcattgt ttccttcaaa atgccagata
    12901 aaagataaaa attctaagta tttcctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaac
    13081 gtagaagtat aatttccttc aacgatccat gacggattag atactataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaatg cggaaagccg gtgatttctt tgatcttcct
    13201 tgcgagggac gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    13261 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg
    13321 actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc aatcagctga
    13381 ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt tggctgttct
    13441 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    13501 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    13561 tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    13621 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    13681 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    13741 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    13801 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    13861 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    13921 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    13981 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    14041 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    14101 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    14161 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat attatcgcta
    14221 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    14281 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    14341 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    14401 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    14461 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    14521 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    14581 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    14641 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
    14701 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    14761 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    14821 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    14881 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    14941 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    15001 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    15061 acaaacttac aagacagtgt tattttatgt gtgtattatt ttatttaata cacatcgttt
    15121 tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattg accttattga
    15181 atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt ataatcctat
    15241 ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac gttcattcgt
    15301 cctcttcatt gatcaacgtt tattttttac atgcaagctt cgcatggcat tcaaaacagc
    15361 taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa aaccaaaagc
    15421 acctaaggaa agtaccaaaa tttttatccc aatagcaaag acaatgtttt gtttgacgat
    15481 tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt gatcattcat
    15541 gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca ttgcgattcc
    15601 tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa atgcggtctt
    15661 tttattgtta ctttgtaaga tttcctctaa ccgctgcact ttatcttctg gaagcaattc
    15721 gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt ttttcgagtc
    15781 gcccgtcaac atgactgtgt ttttcacacc agcctgtttt aattgaacca atgcgtctac
    15841 ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt gatcgattgc
    15901 tagataaagc aatgtaccga tttcttgact tgaaggaaaa gagatttcaa atctttccat
    15961 caatttgtga ttacctacta atacttgttt gccatcaact tcagctaaga tcccttcacc
    16021 tgcaaattcc tggatattag ctgttggtaa aagcggacct gcgtagcctt ctaaaacaga
    16081 ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca atatattgca tgaatatctt
    16141 tggatcgatt tttgtatcga tcgtctggat cgaaaagtcg cctttcgtta atgtgccggt
    16201 tttatcgaat accactgtct cggtttgcgc aagcaactcc aagtaatttg aaccttttac
    16261 taatacaccg attttacttg ctcctccgat accgccaaag aaactaagtg gcacggaaat
    16321 cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga tccattcaga
    16381 aaacgccgct ccagaaacaa caagtggagg taaaacagct aataaaacgg ccaatcctac
    16441 aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa ttttcagctg gtgctttttt
    16501 actgctggca ttttctacta attctaataa tttgctgata gtagaatttt cataagattt
    16561 ttctacttga actaaaaatg gtttattttt attgataaat ccgcttaata ctgtttcacc
    16621 aacatataca gatttaggga ctgattctcc tgtaagtgct gacgtatcga ctaatgattc
    16681 tccttcgatg atcgttccat ctaacggtac acgttcacca ggcttcacaa gtacaaactg
    16741 accgatcagc acgtcttcag ggttcacggt catcaatcct tccgccgtct gcacgtgagc
    16801 tgtttctgga cggatcgcca tcaattcgcg aatcgatttt cttgaatgac tgactgccaa
    16861 tccttggaaa tactcgccga tttgataaaa tagcatcact gctacggctt ctggatattc
    16921 accgatcaac atagcgccaa tcgtagcaac agacataaga aaattctcat caaagatttt
    16981 tcctttgacg atattcatca ctgccttctt cactacatca tatcctatca gcagataaat
    17041 caataaatac cctatcaagc gaattagttc attaaaatca aataatagca ataaagcgat
    17101 acctgcaagg gaaaataaaa tccggaacag cgtatttctc tctttttcat ttccatgttc
    17161 agatactttt tccttatcta tttctgttac tttgacatct ggttctaatt ccttcactac
    17221 tttttttgtt tcttgttcaa tcgtagccaa atgatcttcc tccgcgtcaa tcgttagttt
    17281 tccagaagtg aagtttaccg ttgcttcctt gactccattg attttctgaa ctcctttttc
    17341 gattttcatt gcacagttcg cacaatctaa accttctaat ctgtaacttt tcattttaat
    17401 gacctcctcg cctcaacata tgaatatctc ttcataacta attataaatg aatggttatt
    17461 catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct gcatcggaaa atttcttcca
    17521 tacagatgtt ttggcttatt ctttcttttt ctcttcgatc attgccactg ctcttatcca
    17581 acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc ctgttgctgg
    17641 caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa tccctgcaaa
    17701 atgtccttcc cagatatacg aagctccact acgctttcaa aaatatagaa agagttcttt
    17761 aatgaaaatt atttcaactt tccataaaca gtaaccccat ataaataaaa gacgtaaaag
    17821 tcacatggaa gaacttatac aggaaaacgc atagagtcag gtgaaataca tgtatcccac
    17881 ctgactctat gctttatcta acctttatta ttctctttta tacaatttct gcaccgaatg
    17941 gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc cctatgatcg
    18001 tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg cctgaaacga
    18061 tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt acttctttgc
    18121 caaaaggcgc aaaagaaagg aaggcgatct taaaacattg cataccgatt gggataccta
    18181 tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg attagtccgc
    18241 cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt ctattactgt
    18301 ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt ccagcatcaa
    18361 atattctgca aatttttttt ggatttcttc cgtattttct tttgttacta atgtgtgttg
    18421 gattagtttt cctgatggtc gctgacccac ccgttcttct gactgttgca cgtccgcttg
    18481 cgcaaaaccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt tattcttatc
    18541 atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag taatcagctg
    18601 attgatttga ggtagatctt cttttactgc ccaccgtatt tccatcattt ctcacttttc
    18661 tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc tatccagctt
    18721 tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct tgtatatttc
    18781 gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac cagcaatgcg
    18841 ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc tttacgccag
    18901 ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat gttgcagatt
    18961 cccgcttttt gaagttcatt tagtaatttt cgataaacgg attcaaattg ctgagcagat
    19021 ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt gattccgatc
    19081 aaaatcgata catagtcagg ttgtaaagct aaacaatctt ctcgccagcg tacttgcaga
    19141 tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat cgttggatat
    19201 tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc agttgcttct
    19261 gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg ttttttcatt
    19321 tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat cccataagcc
    19381 ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag tcccttcaaa
    19441 caattgaatg aacgctcatc aagtgtctag ttttttatgc tatttttttc ttaacgtgct
    19501 ggattcccct tgattccttc attgtttggt ccagagagat cgatcacacc taagcttccg
    19561 tcaatcagac cacttgatga cgccatcaga acatatattt ataggctcat ctaagacttc
    19621 cagtaaatgt tcctttacta atatctcttc tagattgctt gggatagatt ggccataact
    19681 ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggatcat tgcatgctga
    19741 aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg tgctagatgc
    19801 tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat aaccttcttt
    19861 gtatatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct
    19921 tttgctttct tctcttatct gctggaaact tcctttatcc cgtatttaaa aaaa
//
LOCUS       DAYYAG010000044        19972 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000044 DAYYAG010000000
VERSION     DAYYAG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.044"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1923
                     /locus_tag="ACHARQ_001384"
     CDS             157..1923
                     /locus_tag="ACHARQ_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8409806.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1938..2375
                     /locus_tag="ACHARQ_001385"
     CDS             1938..2375
                     /locus_tag="ACHARQ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8409807.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2388..3539
                     /locus_tag="ACHARQ_001386"
     CDS             2388..3539
                     /locus_tag="ACHARQ_001386"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8409808.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3601..4299)
                     /locus_tag="ACHARQ_001387"
     CDS             complement(3601..4299)
                     /locus_tag="ACHARQ_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8409809.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4292..5644)
                     /locus_tag="ACHARQ_001388"
     CDS             complement(4292..5644)
                     /locus_tag="ACHARQ_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8409810.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5867..7267)
                     /locus_tag="ACHARQ_001389"
     CDS             complement(5867..7267)
                     /locus_tag="ACHARQ_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8409811.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7428..8165
                     /locus_tag="ACHARQ_001390"
     CDS             7428..8165
                     /locus_tag="ACHARQ_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8409812.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8290..8769
                     /locus_tag="ACHARQ_001391"
     CDS             8290..8769
                     /locus_tag="ACHARQ_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8409813.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8766..9533
                     /locus_tag="ACHARQ_001392"
     CDS             8766..9533
                     /locus_tag="ACHARQ_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8409814.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            9763..10332
                     /gene="lepB"
                     /locus_tag="ACHARQ_001393"
     CDS             9763..10332
                     /gene="lepB"
                     /locus_tag="ACHARQ_001393"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8409815.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10436..11038)
                     /locus_tag="ACHARQ_001394"
     CDS             complement(10436..11038)
                     /locus_tag="ACHARQ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8409816.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11144..11374
                     /locus_tag="ACHARQ_001395"
     CDS             11144..11374
                     /locus_tag="ACHARQ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409817.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            11365..11613
                     /locus_tag="ACHARQ_001396"
     CDS             11365..11613
                     /locus_tag="ACHARQ_001396"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8409818.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            11719..12303
                     /locus_tag="ACHARQ_001397"
     CDS             11719..12303
                     /locus_tag="ACHARQ_001397"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8409819.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12306..13379
                     /gene="prfA"
                     /locus_tag="ACHARQ_001398"
     CDS             12306..13379
                     /gene="prfA"
                     /locus_tag="ACHARQ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8409820.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13372..14211
                     /gene="prmC"
                     /locus_tag="ACHARQ_001399"
     CDS             13372..14211
                     /gene="prmC"
                     /locus_tag="ACHARQ_001399"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8409821.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14204..14383
                     /locus_tag="ACHARQ_001400"
     CDS             14204..14383
                     /locus_tag="ACHARQ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409822.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14405..15430
                     /locus_tag="ACHARQ_001401"
     CDS             14405..15430
                     /locus_tag="ACHARQ_001401"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8409823.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15621..16865
                     /gene="glyA"
                     /locus_tag="ACHARQ_001402"
     CDS             15621..16865
                     /gene="glyA"
                     /locus_tag="ACHARQ_001402"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8409824.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            17018..17965
                     /locus_tag="ACHARQ_001403"
     CDS             17018..17965
                     /locus_tag="ACHARQ_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8409825.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18099..18728
                     /gene="upp"
                     /locus_tag="ACHARQ_001404"
     CDS             18099..18728
                     /gene="upp"
                     /locus_tag="ACHARQ_001404"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8409826.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18867..19109)
                     /locus_tag="ACHARQ_001405"
     CDS             complement(18867..19109)
                     /locus_tag="ACHARQ_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409827.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19230..19302
                     /locus_tag="ACHARQ_001406"
     tRNA            19230..19302
                     /locus_tag="ACHARQ_001406"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19263..19265,aa:Thr,seq:cgt)
     gene            complement(19421..>19972)
                     /locus_tag="ACHARQ_001407"
     CDS             complement(19421..>19972)
                     /locus_tag="ACHARQ_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8409828.1"
                     /translation="FAPSIISIFSLTITGFIAFTLVFGKKDTKKMFSKPVAPVKNIAK
                     YFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYFLAI
                     FSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIKSNS
                     IITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         6791 a         3558 c         4080 g         5543 t
ORIGIN      
        1 gttttattta aaattgaagt tttctgatac cgaaatcttt ttattttgct gacatactca
       61 aacctttggc acaaagtttc gtgccaaagt acgactattt ctctttttag taagcggata
      121 caattagagt atcaaaagga gagagggaga gacaaaatgg aacggacgat tcccaaaaac
      181 aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta ctttatcaag catggtcatg
      241 ccaaatattg gagcactgat tgcctggggt gtattaacgg ctctatttat cccagatggt
      301 tatttaccaa atgaggcttt cgctaccatg gtaagcccaa tgctaactta tctgattcct
      361 ttattgatcg gttatactgg cggaaaagta attgctggtg accgcggatc agttgttggg
      421 gcaattgcaa cgatgggtgt gattgtcgga acagatattc caatgatgct aggcgcgatg
      481 atcatgggac ctcttggtgg atatgccatc aagaaatttg atcagctatt ccaaaaacgg
      541 atcaaatccg gttttgaaat gctagtcaat aatttttcag caggtcttat cggttttgga
      601 ttagcgttat taggtttttc tgcgattggt ccagttgtcg atgcattgac tcaagcaatg
      661 gcaaaaggtg tagagatcat tttatctgcc catttgattc cattgacaag tatttttatt
      721 gaaccagcaa aaatcttatt tttgaataat gcaatcaatc atgggatttt gacaccattg
      781 ggaactgaac aagtagcaga ggctggaaaa tctattctat tcttgcttga agcaaatcct
      841 ggtccaggac ttggcgtatt acttgctttt actttgtttg gcaaaggagc agcaaaatct
      901 tcagcaccag gagccatgat cattcacttt ttaggcggga tccatgaaat ctattttcct
      961 tacgttatga tgaaaccgtt attattctta tcagttatag gcggaggaat gtcaggaagt
     1021 ttcattttcc aattgatgga tgctggacta agagcgccag cttctccagg gtcgatcatt
     1081 gcgattctag cgatgacacc gttcaattct taccttcctg ttattttggg cgtgattgtt
     1141 gccgcagctg tttcgtttgg tatttctgca gtaattttga aagcggatgc gaaagataca
     1201 aatgaagact tcgaaaaatc tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt
     1261 gcccaaggta ttcctcaagg agctacattc tctggcgtac aaaaaatcat ttttgcttgt
     1321 gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa
     1381 gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc aactgacaga tgactcgaat
     1441 acattgatcg ttactcaggt cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg
     1501 cgttttgtct cagtagaaaa ctttttgaat tcacctcgat atgatgaaat cattcaagaa
     1561 ttaagcagca agcagaaaac atcagtttct gaaattgaga atgagccagc agaagagttt
     1621 ctttcagcaa acatcaaaca aatcaaagag attctacttg tccacgatga tcgttccgga
     1681 tcagcaacaa tggggatgac cgtgctcaaa gatatattag acaagaacca tttaactatc
     1741 ccgttaagaa aagtcaatat ccaagattta aaagatgaac ctgattcact gatcgtagcc
     1801 aaagaaagtt tagcggaaaa agtacagatg gctgcacctc aatccattca tttatcagtc
     1861 aacagcatgg tcaatccgca aaaatacgaa tcgattgcac aatatttgaa acagacagct
     1921 taagggagga aaacagcatg caaagtttgc cattcgacat gatcgagctc aatcgatctt
     1981 ttaaaaccaa agaagaagcg atccgctatt gcggtcagat gcttgtagaa gcagggtgcg
     2041 ttgatgagga atatatcgat gccatggtag atagagacaa aatgttatct gtttatatgg
     2101 ggaattttat cgcgattcct catggaacag atgaagcaaa aaaatatgtg aaaaaatccg
     2161 gcatctgtgt cgttcaagta ccagatggtg tagattttgg taccgaagaa gaagaaaaga
     2221 ttgcgactgt tttgttcggt attgcaggag tcggagatga gcatttgcaa cttgtacagc
     2281 aaatcgcact ttattgcagt gatatggaca atgttgtcca gctagcagac gcgctcagca
     2341 aagaagaagt aacggaaaat ctagcaattg cttaaggaga gagaaaaatg agagcagtac
     2401 attttggtgc aggaaatatc ggtcgaggat ttattggaga ggttttggca gctaatggat
     2461 ttgagattac tttcgtagat gtgaatcaca cgatcattga tgctttaaat gaaagaaaag
     2521 aatacgaaat cgaattagca gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag
     2581 gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga aatcgctgaa gcagatttag
     2641 ttactaccgc cattggcccg aatatcttac cttttattgc tgagttgatt gcaaaaggta
     2701 tccaagaaag agaaaaggaa ggaagcacaa catctttaga tatcattgcc tgtgaaaata
     2761 tgattggcgg ttcagcattt ctagaaaaag aagtgtataa atatcttccg gaaacatcat
     2821 ttactgacaa atatatcggc tttccaaatg cagcagtcga tcgaatcgtc ccattacaac
     2881 accatgaaga tcctttattc gtccaagtgg aacctttcaa agaatgggtc attgacgata
     2941 gccaaagaaa aaataaaaaa atccaattaa aaggggtttt atatgtagat gatctagaac
     3001 catatatcga acgcaaacta tttagtgtca atactggtca tgcgactgtc gcttatacag
     3061 gtgccttgtt aggttaccag accattgatg aagcaatgca agatgcgtta gttgtggcac
     3121 aattaaaatc agttttgcag gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg
     3181 cagagcagca tcatgcgtat attgaaaaaa tcattcatcg cttccaaaac aaaaatatct
     3241 ctgatgcgat cacacgtgtc gctcggacac cattacgtaa gttaggttat caagaaagat
     3301 ttacacgtcc agtgagagaa ctacaagaac ataacttgac ttgcccacat ttgacggcaa
     3361 caatggggat tatttttaat tactatgatc ctgaagatga acaaagccgt caattgcatg
     3421 aaatgaagat ccatgaaaac ttggaacaat tgattcaaga agtcacagga atcaatgatc
     3481 cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa
     3541 ataggatacg aaaaagggga atgtgacgaa atttttgtca cattcccctt tttcgaataa
     3601 ctaggctgtt tgtccttcaa gatagggcag atccaattct ttaccgtaac gttgttctaa
     3661 agctaggcgg atcaaacgag ctgccaggtt ctcatttctt gcaaggatag gtccatggaa
     3721 ataagaaccg aagacatttc ggtaaatgac accttcagat tggtcttcac cattatttcc
     3781 ttgaccttgt accactttcc ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt
     3841 gtggttttca aatccataat aagtttcatt gaattcttcg ttatggataa cgatatcacc
     3901 gataaaccgg ttattttcct gacttaacgt gtagtgatcc agtgcactga ttccttggat
     3961 cttttctcca ttagctccca tgtagtagtg acctagcaat tggtagccac cacaaatggc
     4021 taacatgacg ccatcatttt cgatatagtt gatcaaagaa tctttctttt tctggatgtc
     4081 ttttgaaatg attaattgct caaagtcttg accaccgcca aagaaaacca agtcatattt
     4141 atcagcatca aaaggttcat gtatactaac gatctccgta tggcaagaaa cccccatttt
     4201 ttctgctata tatttcaaca tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt
     4261 tccataaagg tgggcgatat tgagttcata attagccacg gttcattcct cctttgataa
     4321 atccttgagc agctagttct ttacgaagct gcaatacagc agtgtaagta gctaaaatat
     4381 agacatgctc agttggcaat tctttgattt tttcgattac ttctggtaat tcttttgttt
     4441 ctgtcaattt attttctgga atcccggcaa cttttagacg taaagtcata tctgtatgac
     4501 ggtctcctcc agaaataaca gctgggatat ccatatcggc aaagttttca aaattaccat
     4561 cccaaatcca gctgacatcg attccgtctg cgtaattggc atttaataaa gccactaaag
     4621 aaaatggata aggagctaga ccaatcatat cgattacttg attgatacct acaggatttt
     4681 tgacaagtac taatgtacat tttttcccat tgatattgat gacttcttgt cggccgaata
     4741 ctttctcgtc atatcctaaa ccttgtttga ttttatcagg atcgacattg aagtactctg
     4801 ccactgcagt agctgctaaa gcattgtaga cattatacat accgccaact tcgattccgt
     4861 attcatgacc atcgatcacg aaatctgctg acacattgtc catccgtacc atatcagtta
     4921 atcgataatc caattcagga cgtttgaagt cacaatttgg acagtaatat ttccctaagt
     4981 ttgcataagt gatcatttta taatgcaaga tgtgatgaca atttggacat aagacaccgt
     5041 ctgtattgta atgagccatt tgttcacgat cttcttcatg gtcaaagcca taatattttc
     5101 taggattgac tgtttcgata gaattgaaga ttggtgaatc accgttactg ataatcgttg
     5161 catttggtga ctttgcggca ccgtctacaa tcattttata ggtcgtatag atttctccat
     5221 accgatccat ttgatcacgg aaaatatttg taaataagaa cagttctggt ttcatatatt
     5281 ctgtgacttt gctcaaactt gcttcatcta tttctaggac agcaaacttt ttctgtcctt
     5341 tttttggttt tgcctgcaag aaagtcgata caattccctg taccatattc gcaccagtag
     5401 ggttggttag aacttcatcg aattgctggc gcaaaatatt gactgttaaa gcggtagtca
     5461 atgttttacc gtttgttccg gtgacaacga cgacttggta gtcttttgcc aaactatcta
     5521 gtatatgtgg atcgactttc agcgctagtt tacctggtaa acttgatccg cctttaaaaa
     5581 aagtttttaa tgcccattgc gacgtttttc caactgcaat cgcaaaatga ctgcgtattc
     5641 ccattatgta acctccaaaa acgatttgct gacgaataaa ttcaactaat cataccatat
     5701 gttgaaaaaa aggttaaatt gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc
     5761 atgaaatcgg acaaaatcgc atgatttaac aaaaaaacag cttgaaacaa aatgattgtt
     5821 tcaagctgct gtgattttat tattccctct tacctatatt cccattttaa ccgataataa
     5881 ttttctcgct tggatattca tagcctagat ctcgtgtttc ttttggcacg aataacatca
     5941 atgtatatag cattccgatc cgcccaataa acatcagtgt agcaatcgtg attttgccca
     6001 caacagacaa gtctccggta atccctaaag ataaaccagt tgttccaaac gcagaagtta
     6061 cttcgatgat gatcgagatc agcgtttgtt cttctgtagc agataaaaaa acgatacaaa
     6121 agaaacacat accaagagaa agcataaaga caacgactga ttttcgcaca tcatcttgat
     6181 cgattctccg tccaaaaata ttgatgttat cttcactttt gaggaaggaa tatagataca
     6241 aaccgatgat tgctacagta gttgttcgaa tcccgccgcc tacagaacta ggactgcatc
     6301 cgataaacat cagtaaagaa aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt
     6361 gaatctgcag accagcatta cgagtcgtaa tcgaataaaa catcgaattg atccatttga
     6421 cactcatatt ggaatcttgg aataaatggt ccttttccaa tagataaatc aagacagttc
     6481 ccgaaacaaa caagataaca aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa
     6541 acggaagctt cgcattggaa cgtttgtata atagccattc ccggcactcc atcaatacag
     6601 gaaaaccaat tcctccgaca aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat
     6661 gtgcaaaggg cttgatggaa tccccagtga cgtcaaaccc agagttcgtg acagccgata
     6721 tcgcctgata aaaaccgtaa aaaacggcat cacgccaacg atcaaaatag cctctgtaat
     6781 aaaaataaat agaaaagaat gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag
     6841 tgatccgaat cagtcggacg ataccagaca aacgcggttg attcatatcc gtcatgatca
     6901 gctgtcgctg tttcagtgtg atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca
     6961 tcatgatccc caaaccacca acttgaaata atatttccag cagtataata ccgttatcat
     7021 tgaacacaga attgatatca aaagtcgata gtccagtcac actcactgta ctgattgcca
     7081 tgaacagcat gtccacaaaa gaaacatggc tatctggttc gcgaaaaatc ggcatataaa
     7141 atagaacgag tgataagacc gtcatcaata tataataaaa aacgatgatt tggatcgatg
     7201 agaaatgatt ggcagcaaac ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa
     7261 catgcacagt actatcctcc tgacaatagt cgtttctacc agtatacaag gggaattagg
     7321 aatactcaaa gaaattatca aacagataac aatacagctt tcgattaaaa tagaaatggc
     7381 ttataataag gaaaatccta gaaaaaagaa ggaggaggaa taacaaattg attaatgctc
     7441 aacaaatcgt agaaaaaatg aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa
     7501 tgatcatgca atgggagaaa gaagaagtaa agcctaagat attaatacat acatgctgtg
     7561 ctccttgcag tacgcacaca ttggaattca tgtctcaata tgcagagatc acgttatttt
     7621 tttcaaattc gaatattcat cctaaaagtg agtatttgcg aagattacat gaacaaaaac
     7681 gatttgtaga ccaatttaat gagaaaactg accatcatgt tgcgctaatc gttgatgaat
     7741 accgtccaaa tgaattcacc aaaatggttt taacaaacca tcttgaaaac gaaaaagaag
     7801 gtggtgctcg ctgcagtgca tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc
     7861 aagaactagg atacgattac tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag
     7921 ctttgatcaa tcagatcgga atggatatcc aaaagattta cgggacacaa tacttgccaa
     7981 gtgatttcaa gaaaaacaaa ggctatgaac gctctttgca aatgtgcaaa gagtatgata
     8041 tttatcgcca atgttattgt ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga
     8101 aagaaatcaa tcgggaagca aaagcttact taaaaacatc aaccgtagtt tttgaggaaa
     8161 attaaagaaa tctttcggaa aaacgtaaga aaaaaataaa gaatttatgg tagaaataag
     8221 gtagactgta taatttgtcc gtagtaaagt aaggaaacaa gaataaattt caatgaagga
     8281 agagataaga tgcgaaaagt agcacctatt ttgaagccaa tcgctgtatt aacaatctcg
     8341 acgattgtcg cgactactac aggtattgtg aatagttcca gctaccgtgc accaagttca
     8401 agttctagta ctgtagaaga aacaacacca actacttcag tctcgacatc tgaaccaaca
     8461 acaacggaaa gtttgaatga aaccactacg acaagtacat gggacgatac aacaactagt
     8521 tcgtcgtatg atgagacata tacaactgac agctcaagcg aaacaagtaa tacggtacca
     8581 gatggcactc catcggattc aaccaatcca agtacaagtc cggatacaag tgctagctcc
     8641 gaaacgatac caagcagtac gactgtagat gataacacgc aaaatgatga tgcagcacgt
     8701 acagcacagc aaaaccaaga ttcaggaaat acccaaacgt ataaccagca agcaggtgat
     8761 tctcaatgac atttttacta gaccttatca acggggtgca taaattcctt ggctatttag
     8821 acatcagtcc taaatatctt aaccgtggat atacgatttt aagtgtgatt cccacgcttt
     8881 atttattgag aattgtttat ggactttggc aaaaccaaaa ctatcttcaa tttttcttat
     8941 atggacttgc atttctagtg cttgtttatt ttactgtttt aaatgtattt tattactttt
     9001 tagataaaaa cacgaaagcc gatgtcactc agctttttgt gaagtattta cctgatgaag
     9061 cgttcaacat ccagacagaa gaatcgatag ataatggaaa ttcgatcgat aaagtgaata
     9121 cacaagaggt catggtttct tatgacgaag attatcaatt gaaattagcc gagaatatgc
     9181 ggtatttgat tggaaatggt gagatcaaaa caaatgaact cggtcgcatg gatggatttc
     9241 tggtcgatag aaatacattg tatccttatt actatgtcaa gaaaacagga gagaaagagt
     9301 atgttctaaa tattggacga agctactcgg acatggaaaa agtagggacc attcgacttg
     9361 actcaacaga agacacattg gaacctgtcg gattgttcat cgtcggcggt gattttgtca
     9421 aagacgggtt acgctatcat gagccttatc gtctgaaatt gattgcaaaa aaggcacagt
     9481 cgcaggaaga accagtggtt tatagtcgta gccaaagaag aagacattca taatgtgatg
     9541 aaacgccgtg tatcagtggt tgcgataaaa acgtttattc ggaataaggg ggatgtgaca
     9601 cgtcttttgt cacgttcctt ttttatataa cgagaaagat aagacgaaca catcacttat
     9661 tctttccttt catttataaa cattgtagaa taaagaaagt acaacgaagt attgtgaaaa
     9721 aacgtaataa ataacgagga aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa
     9781 aattggggga ttttgatcct tgtattagcg gctattttat tagctagagt gtacgtgttt
     9841 acaccagtta ctgtaaacgg gcattcgatg gaccctacat taagtgatgg acaacgattg
     9901 atttcatcta agatctcaaa ctatgaacga atggatatta ttactacaaa ggaaccgggg
     9961 gatgaagaac ggatgattgt taaacggatc atcggtatgc ctggagatac agtcaagatg
    10021 gaaaatgacc agttgaccat taatggcaaa aagtatgatg aaccttatct ggatgagttc
    10081 aaaaaagaat tttcagaaga taagttacaa ggagaatatg cttatagctc aggattccaa
    10141 gcacaagcag aaagttcatc cacctttacg agtgactttg aatatactgt gccaaaagga
    10201 aagtatcttg ttctaggcga taaccgcttg atttcaaaag acagccgaat gtttgggtta
    10261 gtcgacaaag atatgatcca aggaaaagtt gttttccgtt attggcctct ttcagaaatt
    10321 aaaatcatgt gatgaaaaaa taaaaagaag ctgtgacatg ttttttgtca cagcttcttt
    10381 ttccgaaaat tccttcttat ttcttggagt tgctcacttt ttttgcaatc tcattttaag
    10441 ctttttggct gatggataag aaaagcgaag cgtttttgtt cataaatagc ttttccccac
    10501 gatggaaggt atgactagta gcagagacaa agcccgtttt ttccaatcga ttattcaatt
    10561 cttcttggct aaagccatta tggactttcg gatgactaat ttgttcattc ttgtcaaagt
    10621 ctacgatgat caattgaccg ccaggagcta aaatttcata aagttttgta agaatattct
    10681 ccgtatcagg aatgtgtaaa agtacaagtg atagaaggat gagattagct tggacagcag
    10741 gaaattcgac agaagcatca gcgtggatcg ttcggacatt tttcagatct gctgcttgga
    10801 tcttttcttc tgccattttc agcatagttt ctgaagcatc ggcaatgatc agttctttga
    10861 atctttccgc aagcggtaaa ctaacaagcc cagttccgcc tccgtaatcc agcaggactt
    10921 tttggtggct gtcgcgcggt agataagtag ttagttcttc gcgaataatc ttggctaatg
    10981 cttgacggtc aggggaatca taatggttag cgatggaatc aaaaatattt tttgtcatca
    11041 aatagctcct tacgtaaaac tttgaaattt taacttatta tagcacgtga aaagaaagaa
    11101 agcatgctat actcaattca aaaagggaga atctagaaag aatatgccga tatcgtttgt
    11161 aaaagataga gaggaaaaag gaaagtgtgt ccgagaaata ttactagact tgcctgagtg
    11221 gtttgggctt cctgaatcaa cggaaaaata tatcgaggaa tcaagtaaac tacctttatg
    11281 gtgtgaaaaa agaaaagaag aatatttagg ctttatcact ctttcacaga caagtgaaga
    11341 tactgccgaa atctattcta ttgtatggga ataaaaaaag actatcatca ccaaggatta
    11401 ggaacaaaac tttttaaaga agcagaaggt tacgcagcaa aacattacaa atatttgcaa
    11461 gtgaagactg tagacgaggg gcactactct atatatgatc aaactatttg tttctacgaa
    11521 tcattaggat tttcaaggtt ggaagttttt ccaaatttat gggatgaatg gaacccatgt
    11581 ttagttcttg tcaaaaaatt ggaacaaaag tgagcaatag atttaatact aatctgtgct
    11641 tgatgaaaat tctcactgta aaaaatgctg aaaatcaagt ataataccat aggaacaaac
    11701 tggaaggtga aaaagagcat ggcacaacta ttttttaagt atggcgcaat gaatagcggc
    11761 aagacgatcg agatcctaaa agtcgcacat aattatgaag aacaaaataa gccagttgtc
    11821 ttaatgacaa gcggtattga tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga
    11881 ctgaaacgta atgcgatgcc gatatttgaa gaaacagacg tatttgacgt aattcagaaa
    11941 atggaaagca aaccctattg tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat
    12001 gtcattgctt ttacgagaat tgtggacgaa ttggatattc cggttatggc atttggtttg
    12061 aaaaatgatt ttcgaaacga gttatttgaa ggatcaaaat atttattgct ttatgcagac
    12121 aaaatcgaag aaatgaaaac aatctgttgg ttctgccaca aaaaagcaat catgaattta
    12181 cattatattg atggaaaccc tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa
    12241 gcttattacc ccgtttgtcg aaaacattac tttcacccat caatgattac agaaggagaa
    12301 taagcatgta cgatcaatta caagcaattg aagaccgtta tgaagagctt ggagaacttt
    12361 taagtgaccc agaagtgatt tctgatacaa agcgttttat gcagctttca aaagaagaag
    12421 caaacacgcg cgaaacagtc gaagtttatc gtcgttataa agaagtagta caaggaatca
    12481 ccgatacaga agaactgctt ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag
    12541 aagaattatc tgaattaaaa aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc
    12601 ttttaccaaa agatccaaat gacgataaaa atatcatcat ggaaatccgt ggagcagcag
    12661 gcggagacga agcagcatta ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg
    12721 aagcacaagg ctggaaaaca gaagtgatgg aagctaacgt taccggaatc ggtggatata
    12781 aagaagtgat catgatgatc accggagaca acgtatattc gaaattaaaa tatgaaagtg
    12841 gtgcgcaccg cgtccagcgt gttccttcaa ctgaatcgca agggcgtatc catacttcca
    12901 ccgcaacagt tgtcgtgatg ccagaagctg aagaagtaga gatcgatatt gaagacaaag
    12961 atatccgtac agatatctat catgcttcag gtgctggcgg acagcacgtc aataaaacag
    13021 catctgctgt acgtttgact cacttaccaa cagggatcgt tgttgctatg caagatgaac
    13081 gttctcaaat caagaaccgt gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata
    13141 agatccagca agaagcacaa agcgagtatg atgccaatcg aaaatcagca gtcggaactg
    13201 gggatcgttc agaacggatc cggacgtata atttcccgca aaatcgtgtg actgatcatc
    13261 gtatcggatt aactatccaa aaattagatc agattttagc agggaaatta gatgagatcg
    13321 ttgatgcatt gatcatgtat gatcaaacat caaaactgga agctatgcaa aatgggtaaa
    13381 atgacctatc gagaagtcct gtcacgggct tcttcttttt tagaggatca tgggaaagaa
    13441 gcatatagta tccaattttt atttttggaa cgaaaacaat ggaaaaaact tgattggctc
    13501 cttcatatga acgaagagat ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga
    13561 cttctgcttg cagatcatcc acctcagtat ttgctaggat atgcagattt ttatgatcat
    13621 cggttaaagg taactgaagc aacactgatc cctcgaccag aaacagagga gttagtcgaa
    13681 tggtgtttag acgagacgcc tggtgtaccg ttagaagtga tagatattgg gacagggact
    13741 ggagcaattg ctattagttt gaaagcagca agaaaaaatt ggcatatatc tgctgtggat
    13801 ctttcagaag aagcattaga agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc
    13861 agtttttatc atggagatac attaggacca gtaatggatc agcaatttga tgtgattatt
    13921 tctaatccgc cgtacatcag tcggaatgaa tgggaactga tggatgaaag tgtccggtca
    13981 ttcgaaccta aaatggccct ttttgctgag aacgatggat tagctattta tgaaaaaata
    14041 gcagttgaag caccaagcgt actaaaatca aatggaaaga tttttttaga aatcggattt
    14101 cgccaaggtg aagcagtaaa aaaaatattt cagcaagcat ttccagataa aaaagtggcg
    14161 ataaaaaagg atctttttgg caatgaacga atgatccaag tttatggttg aagttgtgga
    14221 tgaaaaattt tttatttgtt tttttattat aaaaaaacac ttatttgaca aggatgtaaa
    14281 cgactttttg gtttatcaac aagtttatcc actattgaca aatagattat ccacaaacag
    14341 tggaaaactt gaggataaac tcagacaaaa taagaatgta tgaaaaaggg gttgtggata
    14401 aactgtggaa acgaaacgat atgatgaaac agaattgcaa gaggcagccg aagcactgaa
    14461 agaaggagaa cttgttgctt ttcctaccga aacagtatac ggattgggcg ccaacgctct
    14521 tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag ataatccatt
    14581 aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat gtggacaact ttcatccggc
    14641 agcagagaag attgtgaaga acttctggcc tggaccatta actttgatat ttaagatcaa
    14701 aaaagacact ttgccctcag ttgtgacggg aggattatcc acagctgcat ttcgtatacc
    14761 ggataacaaa aaaacactag aagtaatcga actttccggt gttccgcttg tcggtccaag
    14821 tgcaaatacg tcgggaaaac caagtccaac gactgcagat catgtatatc atgatctaca
    14881 aggaaaaatt accgggatta ttgatgatgg agctacgcga attggtgtag aatccactgt
    14941 tcttgattta agtgatccaa cagccatgcc tatgatttta cgaccaggtg cagtaacgaa
    15001 agaacaaatc gaagcagtca tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga
    15061 aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac tactcacccg atacacgtgt
    15121 tctgatggtc agagatggcg attggtcaac tgcagttcaa tgggcaaaaa ataagaagat
    15181 ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag atactgcagc
    15241 tgtttatatg tataacgaca actcagtaga agcggcggct aaaggattgt ttgcaggtct
    15301 aagaggatta gatgaaccaa cacttggatt agatttgatt tttgtacaag tgtatccaga
    15361 aactggattg gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac aaaattattt
    15421 tgaaaaatga tcaattggtt attgtcgcat tcttttagta tgatacaatg ctatcaagta
    15481 aatcttctat cagtgaacga aaaactaagc gtaactaatc gtaatagaag agatacgaat
    15541 atttggcaat ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa ttgtaggaaa
    15601 ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat ctttgggctg
    15661 cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc tctgaaaact
    15721 ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa tacgcagaag
    15781 gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt gaaaatttag
    15841 cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag cctcactccg
    15901 gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac acgatcttag
    15961 gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac ttcagtggca
    16021 aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt gattataatg
    16081 tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt gccagtgctt
    16141 acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta ggcgcaaaac
    16201 tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat cctaatccag
    16261 ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc cctcgaggcg
    16321 gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg gtatttcctg
    16381 gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca ttcaaagaag
    16441 cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca aaagcaatgg
    16501 tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca gataatcatc
    16561 tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag agcattttgg
    16621 acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta agtccattca
    16681 aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc aaagaagaag
    16741 atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat gataacgctc
    16801 aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg ttgcataaaa
    16861 aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt ttctgtttat
    16921 tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata tgaggtgtaa
    16981 ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta tctcgttaga
    17041 aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct atgaactagc
    17101 accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga acaaatattc
    17161 cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca atattcgtct
    17221 cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc atggagcagg
    17281 ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg cggttcgtac
    17341 gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat atccaacagc
    17401 aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt caaaaaatct
    17461 ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg ctacagtcat
    17521 gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc tgtattatcc
    17581 agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa actatttttt
    17641 gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag aagagcgtgc
    17701 tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc ttccttcagc
    17761 aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt atgcgcgcaa
    17821 acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca tccatgattt
    17881 tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg cattatctac
    17941 ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt cttttcaaaa
    18001 acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt ttgttttaca
    18061 attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt caagtgatcg
    18121 atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc ggaacaaagg
    18181 tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta tcacgggata
    18241 tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa aaaacgttgt
    18301 ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg gttgacggta
    18361 tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga gatgaagaaa
    18421 cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca cgtcagttgt
    18481 ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc gatgcattga
    18541 aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct gagggagtaa
    18601 aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta gatgaaaaat
    18661 tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt ttgtttggta
    18721 caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct tttttggtca
    18781 tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat aaaaaaacca
    18841 tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta gttaacgatt
    18901 actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc aatctcaaca
    18961 acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc gtgaattgtg
    19021 aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac gatagttgta
    19081 tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa ttataccacg
    19141 ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta caatcagcca
    19201 aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta gagcaacgca
    19261 ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga gaagacagat
    19321 atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga aataaagtat
    19381 gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat aaaacgacta
    19441 gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg atcgagttgc
    19501 ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa aggatgtgtg
    19561 ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat ccaaagatga
    19621 ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata gcgagaaaat
    19681 acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat aataacgggc
    19741 tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt tgcaaaaata
    19801 ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata tttttgacag
    19861 gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca agtgtaaatg
    19921 ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca aa
//
LOCUS       DAYYAG010000045        19825 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000045 DAYYAG010000000
VERSION     DAYYAG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.045"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..561
                     /locus_tag="ACHARQ_001408"
     CDS             79..561
                     /locus_tag="ACHARQ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409829.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            758..1648
                     /locus_tag="ACHARQ_001409"
     CDS             758..1648
                     /locus_tag="ACHARQ_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8409830.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1974..4448
                     /locus_tag="ACHARQ_001410"
     CDS             1974..4448
                     /locus_tag="ACHARQ_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8409831.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4551..6287)
                     /locus_tag="ACHARQ_001411"
     CDS             complement(4551..6287)
                     /locus_tag="ACHARQ_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8409832.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6284..6988)
                     /locus_tag="ACHARQ_001412"
     CDS             complement(6284..6988)
                     /locus_tag="ACHARQ_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8409833.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7119..7622
                     /locus_tag="ACHARQ_001413"
     CDS             7119..7622
                     /locus_tag="ACHARQ_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8409834.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7582..7920
                     /locus_tag="ACHARQ_001414"
     CDS             7582..7920
                     /locus_tag="ACHARQ_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8409835.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7941..9359
                     /gene="ffh"
                     /locus_tag="ACHARQ_001415"
     CDS             7941..9359
                     /gene="ffh"
                     /locus_tag="ACHARQ_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8409836.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9471..10049
                     /locus_tag="ACHARQ_001416"
     CDS             9471..10049
                     /locus_tag="ACHARQ_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8409837.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10053..10574
                     /locus_tag="ACHARQ_001417"
     CDS             10053..10574
                     /locus_tag="ACHARQ_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8409838.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10653..11207)
                     /locus_tag="ACHARQ_001418"
     CDS             complement(10653..11207)
                     /locus_tag="ACHARQ_001418"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8409839.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11460..11735
                     /gene="rpsP"
                     /locus_tag="ACHARQ_001419"
     CDS             11460..11735
                     /gene="rpsP"
                     /locus_tag="ACHARQ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8409840.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11747..11998
                     /locus_tag="ACHARQ_001420"
     CDS             11747..11998
                     /locus_tag="ACHARQ_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8409841.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12122..12913
                     /locus_tag="ACHARQ_001421"
     CDS             12122..12913
                     /locus_tag="ACHARQ_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409842.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13083..13604
                     /gene="rimM"
                     /locus_tag="ACHARQ_001422"
     CDS             13083..13604
                     /gene="rimM"
                     /locus_tag="ACHARQ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8409843.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13594..14355
                     /gene="trmD"
                     /locus_tag="ACHARQ_001423"
     CDS             13594..14355
                     /gene="trmD"
                     /locus_tag="ACHARQ_001423"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8409844.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14491..14838
                     /gene="rplS"
                     /locus_tag="ACHARQ_001424"
     CDS             14491..14838
                     /gene="rplS"
                     /locus_tag="ACHARQ_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8409845.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15148..15390
                     /locus_tag="ACHARQ_001425"
     CDS             15148..15390
                     /locus_tag="ACHARQ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8409846.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(15478..15624)
                     /locus_tag="ACHARQ_001426"
                     /pseudo
     CDS             complement(15478..15624)
                     /locus_tag="ACHARQ_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15681..15995
                     /locus_tag="ACHARQ_001427"
                     /pseudo
     CDS             15681..15995
                     /locus_tag="ACHARQ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16024..17283
                     /locus_tag="ACHARQ_001428"
     CDS             16024..17283
                     /locus_tag="ACHARQ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8409847.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17336..18232
                     /locus_tag="ACHARQ_001429"
     CDS             17336..18232
                     /locus_tag="ACHARQ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409848.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18243..19112
                     /locus_tag="ACHARQ_001430"
     CDS             18243..19112
                     /locus_tag="ACHARQ_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409849.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19122..19797
                     /locus_tag="ACHARQ_001431"
                     /pseudo
     CDS             19122..19797
                     /locus_tag="ACHARQ_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6499 a         3457 c         4108 g         5761 t
ORIGIN      
        1 aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag
       61 tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt
      121 tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg
      181 gagatattag gcctgatcca aggaagcgga ttagtggttt ctaggaattt tccagctttg
      241 actgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac
      301 tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctat gttttctttg
      361 ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggattcagct
      421 tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt
      481 attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta
      541 tctataaaaa ataactattg atggtggatc atcagtaaaa aagcatatga gctgcgtaaa
      601 atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat
      661 gatttttaca taacttaact atatttttac agcaaccata cgagggattt acagtcgact
      721 gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct
      781 aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc
      841 tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc
      901 gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa
      961 ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg
     1021 agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc
     1081 aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac
     1141 agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa
     1201 ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag
     1261 cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca
     1321 agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag
     1381 ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga
     1441 accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta
     1501 cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga
     1561 aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga
     1621 acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac
     1681 tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa
     1741 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
     1801 tttattcgaa aaaaagagct acgacataga actgtcgtag cttttttttt gctatatgac
     1861 aggaatgaaa ggtgtttgca agcttaatgt gaggaacact ttccatatct gttctatact
     1921 ggataggaga agtttggaaa tatccagaaa acgaaatcaa tcgaggtagc cacatgaaga
     1981 ataaatggat aaatcgaagt aaaaaaaaga aagtaacaca aagatcatca gataaagtca
     2041 tccgtctttt tccatcgtat aaagaaggat taacggcaga tcaagtcaag gaacgaatag
     2101 aaaaaggtgc agcaaataat tcagtcgatc ccacattcaa aacaaatcag caaatcgtat
     2161 tggaaaacat atttacgtat ttcaatttga tctttcttat cttagccatt ttgctttgtt
     2221 tagtagaatc ctataagaac ttgacttttt taccagttat catagccaat acaggaatcg
     2281 ccatttttca ggaaattcga tccaaaaaga tcttggacga actaaatgtt ttaaatacag
     2341 caaaagtcaa tgtcatacga gacgggatag aacaaacaat agatatagaa gaccttgtaa
     2401 tagatgacat tgttatttta gagactggac atcaaattcc agcagatgca gaggtcgtgg
     2461 aagggaaact gcaagttaat gaggctttat tgacaggcga agcagatgaa ataaccaaag
     2521 aaataggcga tccattaatg tccggcagtt tcattgtttc cggaaaagct tacgcccgat
     2581 tagacaaagt tggagcagat tcgtatattt cccagctgac agtcaaagcc aagacaatgg
     2641 gagaaggaga acagtcggag atgattgctt ctcttaatca attgatcaaa tggattggga
     2701 tcatcattat tcctatcggt atcctgcttt tttctcaaag ttactttttt aatggaaaca
     2761 cgatcaaaga aagtgtcgtt gcaatggaag cagcattgat cggtatgata cctgaaggac
     2821 tgtacttgct gacgacgatt gcattagcat taagtgcgac taaactagca aagcaaagag
     2881 ttcttttaca caatatgaaa agcattgaga cattggcaag ggtaaatgtc ctttgtgtag
     2941 ataaaacagg aacgatcaca gaaaataaaa tgtctgttca aaaagtgatc gtctctaaaa
     3001 aacaagaaat aatcagtcag tcagatcaat tggaagcatg cattgctgac tatgcaaaag
     3061 cgatggcagg agacaatgca acaatggaag ctgttaaagc atattttaca aaaaccactg
     3121 atcaatccta tcaccatgtt attccttttt cttctgtcca aaaattcagc agtgtagcgt
     3181 tggcagacaa ggtgtatgtt ttaggagcgc ccgaaatggt tttgcgcgat caattgccgg
     3241 aatatgcgga agagtttgtt tctttcgctg aaaaaggcta tcgcgtgttg gtctttggtg
     3301 tgtacgatgg gattcttgaa gaaccagtat tgacagaagc agtcacacca ttaggctata
     3361 ttctgattgc taatcctatc agaaaagaag caagagccac atttgattac ttcaaaaaac
     3421 agcaagtagc catcaaagtg atttctggag ataatccgct caccgtatcc aatgtagctg
     3481 tccaagcagg gatttcacat gctgatcagt acgttgatgc atctaaatta tcagaagaag
     3541 aatatgaagc agctgtcgaa aaatatacag tttttggacg cgtcaagcca gaacaaaaga
     3601 aaatcttcgt tcaattgcta aagcgtcgta acaatacagt agctatgaca ggtgatggcg
     3661 tgaatgatat tctagccatg aaagaagcag actgcagtat agcgatggct tccggaaatg
     3721 aagccgccat gcaggcctca caagtggtct tacttgattc tgacttttca cgcatgccag
     3781 aagttgttgc agaaggacgg cgcgttgtga acaatattga gcgatcagcc agcttgtttt
     3841 tagtgaagaa tattttctcc tttttgctct cactgttttc agtgatcttt gctctgacct
     3901 atcctttaga accatcacaa atcacattga ttagtttgtt tactatcgga cttccatctt
     3961 tcttgcttgc tctagaagaa aacaaaaagc gaatcagagg gaaattcatc atgaatgtga
     4021 tggaaaaagc tgtgccaggt ggactaacag atatgatcgt agtcggtgcg ttggtgatct
     4081 gcggggtgac tttgaatcta aataaaacag atgtttctac agcatctacc atgcttttga
     4141 ttgcagtagg gttcctcgtt ctctataaaa tctgttcacc gctgaataaa ttcagaagtc
     4201 agatcattct tttctgtgct agcggtaact ttttttcggt catctttttg cataagctgt
     4261 tttcaatcac cggtatctca gctgtatcac ttttgctgtt gagcattcta tttttctcgg
     4321 cagattcgat ttttaggcat ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg
     4381 aaaaagagca aaagccgttt agtcgcgtag ttaaaaaatt tctatcgata tttaaacgag
     4441 aaaattgacg aaaagtatct caaaaaagag taaaaaagaa caaccactcg tgtatctttc
     4501 gaagcaggat gagtcatcca actttaaaac gcacgagcag ttgctctttt ttacattttc
     4561 ggaatggtaa cggtgaaagt cgaacctgtt ccaagatgac tgtctacagt aatcgtacca
     4621 tgaaggagtt gcacgtagtc ttttacaatc gctaacccta atccggtccc gcctgaatgc
     4681 cggcttcgcg ccttgtctac gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg
     4741 tcgataccta taccaaagtc ttggacagaa aacgatagat catctgttgc ctgatagcgg
     4801 ataataatct cgctgtcgct ttttgaatat tggacagcat tttcaatcag atttttgata
     4861 attggataaa ggatatctgt tttagtagta aatggttcgt tctttccaat caatcgaatc
     4921 gtcaaccgtt tcttttcgat gattggctga taattttgaa cgatttgctg gaaataaggt
     4981 tctgtataaa ttgtctggat ctcgtaagaa ttctcgccat cttttgacag ctggatgatt
     5041 tcccgaatca gtttatctaa ccggatcgca tctttttgca tgatctcaag aaaagaagtc
     5101 agagtttgcg ggtcatcttt tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa
     5161 gtcacaggag tttttaattc gtgagagacg ttgccaacga aatccttttg tagcttttct
     5221 aaacgtcgca ctttcgttaa gtcatacgcc acccctagaa tctgtccggt accttctgaa
     5281 ttagtaaagt agcgcaaact caagtctaat gtctgccgac cttctgtaag ggtgatctcc
     5341 tgatggatca gcggatgctc aggagtcact tgatgaatca gctgaatgag ttttggctct
     5401 tgaatgactt ctgtgtaccg ctgttcaggt tgatagctta ccacgtttaa atgtagctgc
     5461 atctttggat taagcagtag taaacgagaa tcagcatcat caatcaggaa tacgccaatc
     5521 atcagttcgt tcagtaacgt atataattgc tcttctgtgg tagtatacgc ccgatacatc
     5581 ttactcattt gttcgctcaa cgcattgatc gtctgataca attcttccca ttgatcgggt
     5641 gtttgcatga ttacttccgc ctgattagga ttttgcacca ttttttttag tacgggcagc
     5701 acggttttca atggctggtt tttttgatag atcagataat agatcattcc tgcaagaaca
     5761 aggaagaaag acagaaagaa gaaaaagacc caacggcgaa aactctcagt tctcggtaaa
     5821 aaaccgcttg tcggttctgc gatacgtaaa atggcttcta actggccact ttttttgact
     5881 ggtaatgcga catataaaag atccccattc aaagtagtac ttttgcgcaa agcgctacct
     5941 aggttcccac cattcaatac ggctttgatt tcgggacggg agtttcgctg ttcattcaat
     6001 gtttcattgt ttgtgtcaaa taagatcttt ccagtagcat ccattaaagt aatccgttcg
     6061 ttcgactgat ggacgaattc ttccaaagca gttttgtttt gcgctgcttg gaggttttcg
     6121 acatctaatt gccggatcag cagttgtgct ttttttgtca ggtagctttc ttgctgttcc
     6181 acgatctgct gttgaaaata atgggaaatc aattgccagc caccaaaaaa caaactaact
     6241 aaaagaataa gaaagaagag acgttcataa atttttttcc atttcatcgt ttgggttcct
     6301 gaaacttata gccaaatcct ctaatagtca ccaagtactt tgggtgtttt ggatctcgct
     6361 cgattttttc tcgcaaatga ctaacgtgta catctacgat tcggctttgt cctgcgaagt
     6421 caaaattcca aatgcggtct agcaatgtgt ctcggtcgat cacacggtct tttcgtttca
     6481 tgaaataaac aagtaattca aattctttcg gtgttagttc aatcagatgg tcgtctacgg
     6541 tcacttgata attggtcaaa tcagcagtga tttgtccaat cgatagatat tcaggctcag
     6601 cttcatcttt tgattgctta cgaggttcaa ttcgtctgaa tattgctttc atacgggcta
     6661 atacttcgcg aggactaaat ggtttggtca aatagtcgtc agcacctatt tctaggccaa
     6721 tgatccgatc cacttggtca tctttagccg tcagcatcaa aatcggtgtt tcattttttt
     6781 cttgacgcag ccgctgagtg atctccatgc catccatcgt tggaagcatc acatctaaaa
     6841 tgatgaaatc aaatgattgt tctaatgcta attctaatcc ttcgcctcca tctgtggcac
     6901 tggttacctt gtagccttct ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt
     6961 catcatcaac aactagtatt tttttcattc aaatctcctt taaataggtt tcattctgtt
     7021 tatattttag catagaatca gtacaaaggg ctattttaca aaagaaataa atggtataat
     7081 aggaaggcac tttgctgaat agaaaggaag agcagtcaat gattggaatc agtgcatgtt
     7141 taggcggtct ggcctgtcgc tatgatggac agaaaaaaga agtaccagaa ttgatggaac
     7201 tagttgaaac tggagaagct gtcatggtct gtccagaagt gatcggcgga ttaccaattc
     7261 ctagagatcc cgctgaaatc attggtggaa atggattcga tgtgtggaaa gatcaagcaa
     7321 aagtatggac gacaagcggc gaagatgtaa cggaagcgta caaaaatggc gcgatcaaag
     7381 cctatcaaaa attgcaggaa aaacacattg atctagtcat catgaaggaa aaaagccctt
     7441 catgcggaac ccattcgatt tatgacggga gtttttccgg aataaaaaaa aatggtgccg
     7501 gagtggctac tgcttatttt attcagcaaa aaatgactgt gctctctgat gaagaatggc
     7561 tgaaaactag aggtgagcag aatggaaatc gaaaagacca atcgaatgaa cgcgctcttt
     7621 gaattttatt cgaccttatt gacagaaaaa caaatgaact acatggaatt atattatgca
     7681 gatgattttt cattaggtga gatcgcggaa gaatacgaag taagccgaca agcagtatat
     7741 gacaatatca agcggacaag caagatcttg gaggattatg aaaaaaaact tcatcttttt
     7801 tctaattata ttgttcgaga acaggtattg gaaaatatga ctgcatatat tacagataag
     7861 tacccggaag ataaaaaact acttgaatat gtacagcaaa ttcaagcaat cgaagaataa
     7921 ataaaaggaa tgaagaaaat atggcatttg aaagcttaac tgaacgtctc cagcaggcga
     7981 tgggaaaaat aagaaaaaaa ggaaaagtat cagaagctga cgtaaaagaa atgatgcggg
     8041 aaatccgcct tgccttacta gaagccgatg ttaacttgca agtagtcaaa gattttacta
     8101 agcgcgtacg cgaacgtgcg gtaggggcag aagtgctgga aagtttatca cctgcacaac
     8161 aaatcgtaaa aatcgttgat gaagaattga cgatcacact tggttctgaa acagcagagt
     8221 taaataaatc accaaaaata ccaactgtta tcatgatggc tggtcttcaa ggggcaggta
     8281 aaacgacctt tactggtaaa ttagccaatt atttaaagaa aaatgaaaat gctcgtccgc
     8341 tcctaatcgc aggggacgta tatcgaccag ctgcgatcga ccaattgaaa gtattaggtc
     8401 aacagctaga cgttcctgta tttgatatgg gaactgaagt cagcccggta gagatcgtcc
     8461 gtcaagggat ggagttagca aaagaaaaga aaaacgacta tgtattgatc gatacggcag
     8521 gacgtctgca tatcgatgaa acattgatgg atgagttgaa acaaatcaaa gaattgacgc
     8581 aaccaaatga gattttgctt gttgtcgatg ccatgactgg tcaagatgcc gtcaacgtag
     8641 ctgatagctt caaccagcaa ttaggtatta caggggtcgt catcactaaa cttgatgggg
     8701 atactcgagg cggggcagca ctatcgatcc gctcagtgac aggcgcacca atcaaattca
     8761 tcggttctgg tgaaaaattg actgacttag aagtattcca cccagatcgt atggctagcc
     8821 gtatccttgg tatgggggat atgctgacat tgattgaaaa agcgcagcaa gactatgacg
     8881 agaaaaaagc ggaagaactt gcacagaaaa tgcgggaaaa ttcattcgat ttcaatgact
     8941 ttattgaaca gcttgatcaa gtgatgggta tgggcccact ggaagatcta atcaaaatga
     9001 tcccaggaat gaatcaagtc cctggaatcg aaaacgtcaa ggtagatcca aaagatgttg
     9061 aacgcaaaaa agcgatggtt tattctatga cgccagcaga aagagcaaat cctgatctgt
     9121 tgaacccaag ccgtcgtcgc cgaattgctg ccggttctgg aaacagtgtt gttgaagtga
     9181 atcgaatgat caaacaattc aaggaatcaa gaaaaatgat gcagcaaatg tccaaagggg
     9241 atatgaacat cccaggtatg gaccaaatgt ttggttcagg cgtcaaagga aaactaggca
     9301 aaatggcgat gaatcgaatg atcaagaaaa ataagaagaa aaagaaaaaa cggaaataag
     9361 atgaaaaaaa cagcttcggt acagactgaa aatgtctact gccggagctt ttctatcagc
     9421 aatttttatt ctttactaat acaattactc taaacgaaga ggtggcacag atgagatgtc
     9481 cgtggagtga acaaacagaa agtatgcggc aatatcatga tactgtctgg ggtgttcctg
     9541 aatatgatga ccaaaagctg ttccgaaaat tgatgcttga tattaatcaa gccggattga
     9601 gctggcagac gattttaaat aaaagtgaag cattcgatga agcatatgat cgattcgata
     9661 ttaaaaaagt cgcaaattat ggagaaacaa aaatcagtga actaatggaa aacaaaggaa
     9721 ttattcgtaa ccgaagaaaa atcgaagcag ccgtccaaaa cgccaaagca gtcttgaaaa
     9781 ttcaagaaga aatcggcagt ttttcagagt atctttggtc gttttctaat ggaaaaatcg
     9841 ttgattcttc ttatcattca caagaagaag tcccaacaac aagtcctctt tcagatgaat
     9901 tagcaaaaga tctaaagaaa agaggattta aatttatcgg aagcacaaca atctatgctt
     9961 ttttagaagc tgtcgggatc atcaatgatc accttgacag ttgtttccgc aaagaagagg
    10021 tcgtacgtat cggcagaaag ggcgaataaa gcatgcgaaa aatcatattt tatggtgcta
    10081 taagtttaga tggctatttg gcaacgaaaa cggatgatct tcaatggctt tttgacactc
    10141 caacaggtaa gcaaaccact tatgaagctt tttatgaaac aatcgatacg accatcatgg
    10201 gcagaaaaac ttatcaggaa gcaaaaaaat atttggagac cgaagcaatt tatccagaga
    10261 aaaagaattt cgttttttcc acccacgcac aattacaatt gccagatgct gaggtcgtaa
    10321 acgaagatcc tgttgctttt ctaaaaaatc tacaaatgac agaaggaaaa gatatctggg
    10381 ttgtgggtgg cggtcgattg atcaaaccgc tattggaaca tcaaatgatt gatgaatggt
    10441 atatccagat tacccctgta ttattagggg acgggattcc tttattccaa aaaggaacat
    10501 atgaagcgcg ttttgaaatg ctagacacga cacgattcgg tgaatttatc gaattgcatt
    10561 atgtacgaaa gtagaagtaa ataaaataaa aaaggcaact atatgcagaa atgtcctgta
    10621 catagttgcc ttttttatta ccgttatgct ttttactctg ttaatttgtt agaaaaatct
    10681 agatacgcat ctaaaatcgt ttggaagaaa cttaatgttc cttcaacgat attaccattt
    10741 tcatcaatta gattcgcaac gtttccgata tatgcttccg gttgctgcaa ggttggcaca
    10801 ttcaaaaaga caagtgattg tcgtagatgg tggtttgcgc caaagccgcc gatcccacct
    10861 ggtgatgcag taacgactaa acctggttta tgatcccaaa cactgtgtcc ataaggtctt
    10921 gagcctacat ctaaagcatt tttcaatact gctggcacag aacggttgta ttcaggagta
    10981 acgaaaacga caccgtctag tcccctcact tcctcacgaa aacgagtcca ttcagcagga
    11041 gcctgttctg gagtttcgag atcctcattg taaaatggca gatcatcgat ttttacaaat
    11101 acactttcgt acccttcagg aagcaatttc gcaaaagctt ctgctacttt tttgttgtaa
    11161 gaatcttttc ttaaactacc aataaaaaat ccaatttttt ttgtcatacg atttttcctc
    11221 cttcaaacta tctataacaa tcttacaaaa aataagtaac tggagcaaat aaaaacactt
    11281 actgacaaaa gctacataga agatagatta cttattttta acaaaaaaga agtctaaaat
    11341 cttctatttc ctaaaaaaaa tcgaatttaa tcaagctgta aagaaaaaaa gctttacagg
    11401 gtaaaaaaaa actgttacac tacatcttgt gaataaaaca atggaggtgg aaataaataa
    11461 tggcagttaa aatccgttta aaacgtatgg gttctaaaaa gagtccgttt taccgtatcg
    11521 tcgtagctga ttcacgttct cctcgtgatg gacgtttcat cgaaactgta ggtacttaca
    11581 atcctttgaa agaccctgca gaagtagttt taaaagaaga cttagttctt gactggttat
    11641 caaaaggtgc acaaccttct gacactgtac gtaacatcct ttcaaaagaa ggcgttatgc
    11701 aaaaacacca cgaagctaaa ttctcaaaga aataaggtga ccggatatga aagatttaag
    11761 cgacttagtc ttaacaatcg ttcgtccgct agtcacttat cctgatcaag tcaagttaga
    11821 cgtcgtagag tcgaaagact tttacgagta caatctaaca gtggctcctg aagatattgg
    11881 ccgtatcatt ggaaaacaag ggcgtgttgc taaagcaatc cgcacaatcg tttacggtgt
    11941 tcggatcaat gcaccgaaaa aagtgcgttt gaatatcatc gatgataagg aaaagtaaat
    12001 aaacgtgctg tactcgaatt ttcgagtgca gcttttttgt ttttttatta attgaagaaa
    12061 tgattatttg gcaaaacaag caagaaagcg tatccttata ggaggaggga gtgaacagcc
    12121 aatgagaaag aaaatgttat ttattccatt tgctctattg ttaagcgctt gtagttcggt
    12181 tactcaagaa tcagacaata cacatctatc aagcagtata tctgaaactg ttaccagtcc
    12241 agaaaaaagt agtaccaaaa cggctaccac cactgaacaa acaatggcta cctcaaataa
    12301 tgagaaaaag actgctttag accagttgaa agaacaacag ccaaatgttc ccatgcctct
    12361 agatgtacca gtctcttcag gctatctcaa tatcgcagcg acacatacaa agcaaggtta
    12421 ttctatttta tattacagaa cagatcgtcc cctcgggttg aatgctgacg aattgaatca
    12481 agaaacgcct attgcaacct atctatatca atatgggttt gcttctagtc aagaaacaat
    12541 ccaagttttg cagccctttg agatcgacac aaatggtcag caagtggact taggttctcg
    12601 aatcacaggg tatcagcaag gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa
    12661 ctggcgtatt cgaatccgtg ggaataatat agaaggccaa gatcccttac tattagctaa
    12721 agaaatcgta gcttatttgg aagaaaacag tctaccagca cctgaacaat ttggtaaaat
    12781 tactgttgat atgggtgata cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa
    12841 aaatgcctat acgatcactc atcaagatcc tatgtctgcg ttgaagatgg cagtttctat
    12901 gaagaggttg tgaaatcagc gcgctgatct gagcccatca tttaactcca agtcacagga
    12961 agtaatcaaa aatagcacgc cgcatttttg cataattttg gcttaatacc ttaaaagaaa
    13021 actagaattg cctcttcaca catgctaaaa taaaacagac aaaaaagaaa tggggatata
    13081 agttgactga atacttgaat gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag
    13141 tccgtgttat ctcaacaacg gatttcccag aagaacgtta caaaaaagga acggtgttaa
    13201 cactattcca agacggaaaa gcacctgtgg aattaacagt caaaagtcat cgcaaacata
    13261 aaaattttga cttattgagt tttgaaggac atccttctat caatgatgtg gaaaaatatc
    13321 gggatggcat attaaaagtg tcaaaagaca actctgtaga tctagctgaa aatgaattct
    13381 attaccatga gatcatcggt ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa
    13441 aagaaatctt gtcaccaggt gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg
    13501 atatattgct tccatatatc gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg
    13561 tccgtgtaga aattccagaa ggactgattg acgatgaaga ttgatgtatt gactttattt
    13621 ccaagaatgt ttgaaggtcc tatgggggaa tccatcatcg gtaaggctgt agataaagga
    13681 ttattagata tcaatatctc taacttccgc gattattcag ataacaaaca tcagacagta
    13741 gatgattatc catacggcgg tggagcaggt atgctgctca aagtccagcc catctatgat
    13801 aacatccaag ccattgaaaa agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac
    13861 ccagctggta aacgattcga tcagaaaatg gcagaagaat tttcacaaga agaacactta
    13921 gtattcattt gtggtcatta tgagggatat gatgaacgta tccgcacatt agtgacagat
    13981 gaagtttcct taggagatta tgtccttact gggggagaat taggtgcgat ggtcatgatc
    14041 gatgcaacag ttcggctttt gccagatgtg ttagggaatc agacttcagc tcaaacggat
    14101 tcttattcaa ccggtttatt ggaacatccc caatatactc gcccggcaga atataaaggg
    14161 atgaaagtac cagaagtatt gacaaatgga aatcataaat tgattgaaga atggcaatta
    14221 aaagaatcac taagaaggac ctatcaacgt cggcctgatc ttttggaaac acttgaatgg
    14281 acacctcaaa tgacgaaatt tttagaagaa atcaagaaag aagaacaaga aaaaacagca
    14341 gatgaagcca agtgactttt tctaaaatat tctttacagt aggttgataa tatgttagta
    14401 ttttatggtg agtgcaaaag cacttaacta ttacgatatt ccgctatgag aaaagctcat
    14461 aagaatattt ggaataagga gaagaaaacc atgaatccat taatcgaaga attaacaaaa
    14521 gaacaattac gttctgacat cccagcgttc cgccctggtg acactgtgcg tgttcacgca
    14581 aaagttgttg aaggtactcg tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc
    14641 cgcggtgctg gaatcagcga aacttataca gtacgtaaag tttctaacgg agtgggcgtt
    14701 gaacgtacat tcccattgca tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt
    14761 aaagtacgtc gtgcaaaatt gtactaccta cgtgcattac acggaaaagc tgctcgtatc
    14821 aaagaaatcc gtcgttaatc attgttatca gactgagaaa gaacccttgg tattcaaggg
    14881 ttcttttttc ttagcatgta atttgaacac cctgatccag tttttttctt taattattgg
    14941 taggggagaa taatggatta gaataaatct acctataagt agtagtaaaa aaaagagcac
    15001 tatgataata tctttcttaa gagtaaccgc tttaataaaa aatccagaat attctaacat
    15061 aaatggagta ttcttagaat caaaaggaga aagaagatgg tttatactta tacggagcaa
    15121 gaaaaaatga aagtttgaga cacattcatg aatgatggaa aagtaaaaca agtaccaagt
    15181 tcaacaaaaa agaaaaacat cctattgaaa gaagtcttga aacgctttga ccatggagtg
    15241 acttatacag aaacagaggt taattcgata ctgctgaacg tattttctag tggcgattat
    15301 gtcgaacaaa gacgttatct aatcacattt ggctttttca aaagaagttc agacggtcgg
    15361 gcatatcaga tgatgggaat tgaaaattag aagttttaat ttatactcat tgaaaaaagt
    15421 tcaagtatgc gatattggtt ctacaccgca tacttgaact ttttttgtca tctgtatcta
    15481 taatacaaag ccaagaacgt tattcaaaat atggatagct atgataaaag ctaatgagcg
    15541 attcgcagtt aaataagcaa tcgtcagtaa aatcgttcca ggagcgtaca tgaaaaaatc
    15601 aattggatgc gttacatgaa taaagataat tctcttttat ttgtacgaat aaataccaaa
    15661 acgtacctcc taaaattaga ttcaaagaag atagcaaaga ggaaaaagat agttctgcag
    15721 cagctatagc ggcatgtgga ttgctagaac tttccaaaca actgcctgtt tcagatcctc
    15781 gtcatttcga ttacgaagag atagcaatca aaatcttgga agaattacag aaaaactata
    15841 caacgaaacg agttaacgga tcaaacggtc tgcttctcca tgccgtttat gataaaaaca
    15901 gcttaaaagg tgtagatgaa tgtgtgatct ggggcgatta cttttatgta gaaggaatca
    15961 ctcgtctagc taaaacatgg tactgttact ggtgatgtat aagagaagag gaggattcat
    16021 ataatgaagc gatggaaaaa aatgatgatt ggatcaatag ttttaggaat gtcactcgta
    16081 tttggtgctt gtggtaatca agaagacagt agtgaagaat cagcaaatga aattaccgtt
    16141 tcgacatgga attatgaaac aacacctgaa ttcaaagcgc tctttgaagc attcgaaaag
    16201 aaaacgggaa tcaaagtgaa agcagtagat attgcttcag atgattatga tacgaaactc
    16261 accacgatgc tttcttctgg agacacgaca gatgtattga cgatgaaaaa tcttttgtcg
    16321 tattcaaatt atgctttacg agatcagtta gtcgatcaaa cagaacgaat tagagaatta
    16381 gacactgatg ctgctgaaga aacatatgaa atgtatgata tcgatggaaa aacatatgca
    16441 ttgccatatc gaacggactt ttgggtacta tattacaata agaaaatgtt tgatgatgca
    16501 ggaatcgaat atccaaaaaa tctaacttgg gacgaatatg aagatttagc aaaaaaactt
    16561 agtaaaaatg acggacaagt ttacggagca tatcaacata tctggcgttc gacaatccaa
    16621 gctattgcag ctgctcaaaa cgaggcgaat cttgttgaac cagattatca atatatgact
    16681 gattattatg atcgagcatt gaggatgcaa aaagaaggcg cgcagatgga ttttggcacg
    16741 gctaaatcaa cgaaagtaac ctatcagtct caatttgaag aacaaaaagc agcaatgatg
    16801 tacatgggaa cttggtatat ggctggcata ctcgcaaaca aagaagcaag taagacagaa
    16861 gtagaatggg gaattacagc tattcctcag aagaaaaaag gagaatcggt cacgacgttt
    16921 ggctcaccaa cagcatttgc tgtcaataaa aactcaaaga aacaaaaagc ggcgcaagaa
    16981 tttattgaat ttgcagccag tgaagaagga gcaaaagtat tagcaggagt aggagtagtt
    17041 ccttcatatc gtacggatga gattgatcag ctttatttta atctcgctgg aatgccaacg
    17101 gatgaaattt ctaaaaaagc tttttctcct gatgaaatca aacttgaatt tccgattgat
    17161 acgtatgggc ctgcaatcga taagatttta caagaagaat atgacttagt tttggttgga
    17221 gatgaaacgc cggaaaaagg aacggtaaat atggagaaac gtgtagctgc ggaaaaagaa
    17281 taatcatgaa aggagagaag ccaagagata aaacactctt ggtttcgtca tcgaaatgga
    17341 gaatagtata aataagaaag ctacaaatca tttgcgtaga aaacgaacgt ggatcgctta
    17401 ttcgtttatc gccccgaatt tcattggttt tttcgttttt acattaatac caatcctgtt
    17461 ttcgcttgta ctagcattta tgaaatggga ttctttttca acgccagaat ttgctggact
    17521 agataacttt cgtaaaatgg ccaacgatga gacattttgg atttctttga aaaatacttt
    17581 tttatatacc gctgctgtag tgcctggcac acttgtttgt tcgttagggc tagctttact
    17641 actaaatcag aaaattagag ggatgaagct ttttcgtacg atatttttct ttccgtatgt
    17701 tacttcctta gtagcaattg ctgttgtttg gaatatgttg tttcatccaa cagtgggacc
    17761 tattaatatc tttttaagac gatttattga acatcctcca ggttggactt caagttcaaa
    17821 ttgggcgctt tgggcaattg taattgtcag tatttggcga gggatgggtt attacatgat
    17881 tctatacctt gcaggactac aaagtatcca aaaagaacta tatgaagcgt cttcgctaga
    17941 tggagcaaac cgttggaaac aatttttgaa tgtgacatta ccttcgttga gaccaacaac
    18001 cttctttgta acgatcatgc ttgtcatcaa ttgtttcaag gtgtttgacc ttgtgcaagt
    18061 gatgacggat ggcggccctg gtcgagcgac aaatgtgctg gtttaccaaa tctacaatga
    18121 agcattcaaa aaatttgatt ttggttatgc ttcagctatt tcacttgttt tgttcgtaat
    18181 tgttttgggc gttacggttg ttcaattttc ttggaataag cgtcgtactt aggaggtgtg
    18241 atatggaaga gagtgttcga aatgaaagaa aaaaagcaaa aattcgttca aaaaacatgc
    18301 cctttattca atggttgggt atgattctgc tgattgtatt agctttattg acgatcattc
    18361 cttttttatg gatggtttct gcttcattca aagcaaataa tgaagtgtta acgattccga
    18421 ttcaatggat ccctaaaact tggcatttag agaattatct ggttatttgg tctcgtattc
    18481 ctttagtgac attttttaag aatacagctt ttttaagcgt tgtgatcacg ttgattcagt
    18541 tgtttacttc tagttttgct gtttacgggt tttctgaact aaattttaaa gggcgcgata
    18601 ttttattttt agcatatatc ggcacgattg ctgtcccttg gcagtcttat atgatcccgc
    18661 agttcattat gatgagaaaa gtgggtttaa cagatacgtt gtggtcgttg gtgctattac
    18721 aagcgttcaa tgcttttggt gtgttcttgt tgaagcaata ttacagtagt atcccagatt
    18781 ctttatgtga gagtgcgcgg atcgatggct tatcagagtg ggaaatctat cgcaagatca
    18841 ttttacctct tacaaaaccg gcgattgcca gtttgacgat catcacattc gttaatacat
    18901 ggaacgatta tatggggcca ttcatttatt tgtcttctac ggaaaataaa acaatccagt
    18961 tagggttgaa aatgtttatt gggctatttg atgcagagta cgcattaatc atggctgcct
    19021 cagttgtttc tgttcttcct gttgctttta tatttttagc gatgcaaaaa tattttgtag
    19081 aagggatcgc ttcatcagga atgaaggaat aggaggtgat tatgaaagag cagcgttttg
    19141 acaaaaatgt ttatatggaa ttcttttatt ggttgtatca atcattggca agtagccttt
    19201 gtttttggtg tgtcaatatc ttgtatatag cagctgcgtt ccttttgtcg atagatggaa
    19261 ggaatgtctt ttcctttatt ttttctttac tttttattgg tccaggttgc atcgtgatga
    19321 taggtatgac agaagaatta gcaaaagaaa aaagtttttc accagtacgt ttgttcataa
    19381 agacgtttcg ccaattttgg ctaaagggat ttttttattg gctatttgct tggatagtta
    19441 gtgtgataat gatttttgat tgtttctttt ttatacgttt ttcttatgga aaatggttga
    19501 ttccgttgtt tgttttactt gcttgtttta cttgcttgtt tatcagtttc ctttagcatc
    19561 aactgctggt attttcaggt gagaaatcca gcgtcgaaac caaatcaagt tctacggata
    19621 gcgttctact atacgttgaa aaaatggtat gtgagtttac tagatttttt actattgaca
    19681 agtttgtttc tcttcttttt cgtaaagccc caatggtgta ttctactagg tccttctatc
    19741 gtatttggtt tgatttactt caacaatcgt aaattgatga gaactatgga tctgtagact
    19801 ttataaattg aaactttcaa ttaag
//
LOCUS       DAYYAG010000046        19629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000046 DAYYAG010000000
VERSION     DAYYAG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.046"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHARQ_001432"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHARQ_001432"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8409850.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHARQ_001433"
     CDS             complement(1172..1867)
                     /locus_tag="ACHARQ_001433"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8409851.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHARQ_001434"
     CDS             complement(1880..2596)
                     /locus_tag="ACHARQ_001434"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8409852.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHARQ_001435"
     CDS             2926..3534
                     /locus_tag="ACHARQ_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8409853.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHARQ_001436"
     CDS             3859..4182
                     /locus_tag="ACHARQ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8409854.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHARQ_001437"
     CDS             4364..5734
                     /locus_tag="ACHARQ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8409855.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHARQ_001438"
     CDS             complement(5864..7165)
                     /locus_tag="ACHARQ_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8409856.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHARQ_001439"
     CDS             complement(7519..9510)
                     /locus_tag="ACHARQ_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8409857.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHARQ_001440"
     CDS             complement(9654..9920)
                     /locus_tag="ACHARQ_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8409858.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHARQ_001441"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHARQ_001441"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8409859.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHARQ_001442"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHARQ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8409860.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHARQ_001443"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHARQ_001443"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8409861.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHARQ_001444"
     CDS             complement(14080..14601)
                     /locus_tag="ACHARQ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8409862.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHARQ_001445"
     CDS             complement(14604..15446)
                     /locus_tag="ACHARQ_001445"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8409863.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHARQ_001446"
     CDS             complement(15439..16164)
                     /locus_tag="ACHARQ_001446"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8409864.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHARQ_001447"
     CDS             16340..16636
                     /locus_tag="ACHARQ_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409865.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHARQ_001448"
     CDS             17204..18796
                     /locus_tag="ACHARQ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8409866.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHARQ_001449"
     CDS             18809..19549
                     /locus_tag="ACHARQ_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8409867.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DAYYAG010000047        19548 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000047 DAYYAG010000000
VERSION     DAYYAG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.047"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(541..1566)
                     /locus_tag="ACHARQ_001450"
     CDS             complement(541..1566)
                     /locus_tag="ACHARQ_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8409868.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="ACHARQ_001451"
     CDS             1841..3526
                     /locus_tag="ACHARQ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8409869.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="ACHARQ_001452"
     CDS             3540..4823
                     /locus_tag="ACHARQ_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8409870.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="ACHARQ_001453"
     CDS             4893..5762
                     /locus_tag="ACHARQ_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409871.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="ACHARQ_001454"
     CDS             5759..6595
                     /locus_tag="ACHARQ_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8409872.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="ACHARQ_001455"
     CDS             6621..7688
                     /locus_tag="ACHARQ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8409873.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="ACHARQ_001456"
     CDS             7685..8512
                     /locus_tag="ACHARQ_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8409874.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="ACHARQ_001457"
     CDS             8557..9582
                     /locus_tag="ACHARQ_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8409875.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="ACHARQ_001458"
     CDS             9579..11762
                     /locus_tag="ACHARQ_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8409876.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="ACHARQ_001459"
     CDS             11762..12892
                     /locus_tag="ACHARQ_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8409877.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="ACHARQ_001460"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="ACHARQ_001460"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8409878.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="ACHARQ_001461"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="ACHARQ_001461"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8409879.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="ACHARQ_001462"
     CDS             complement(14378..15064)
                     /locus_tag="ACHARQ_001462"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8409880.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="ACHARQ_001463"
     CDS             15144..16682
                     /locus_tag="ACHARQ_001463"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8409881.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="ACHARQ_001464"
     CDS             16802..17269
                     /locus_tag="ACHARQ_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8409882.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17803..18231
                     /gene="rplM"
                     /locus_tag="ACHARQ_001465"
     CDS             17803..18231
                     /gene="rplM"
                     /locus_tag="ACHARQ_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8409883.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="ACHARQ_001466"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="ACHARQ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8409884.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..>19548)
                     /locus_tag="ACHARQ_001467"
     CDS             complement(18727..>19548)
                     /locus_tag="ACHARQ_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8409885.1"
                     /translation="TKKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSITA
                     PKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKS
                     ETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQA
                     DQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLR
                     HTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         6330 a         3530 c         4149 g         5539 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatcctttt atgattttta tatttgcata ttctttcgat tttttggt
//
LOCUS       DAYYAG010000048        19522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000048 DAYYAG010000000
VERSION     DAYYAG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.048"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHARQ_001468"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHARQ_001468"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8409886.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHARQ_001469"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHARQ_001469"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8409887.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHARQ_001470"
     CDS             8118..9086
                     /locus_tag="ACHARQ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8409888.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHARQ_001471"
     CDS             9104..10009
                     /locus_tag="ACHARQ_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8409889.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHARQ_001472"
     CDS             10006..10812
                     /locus_tag="ACHARQ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8409890.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHARQ_001473"
     CDS             10969..11937
                     /locus_tag="ACHARQ_001473"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8409891.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHARQ_001474"
     CDS             complement(12254..12778)
                     /locus_tag="ACHARQ_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8409892.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14876
                     /gene="scm"
                     /locus_tag="ACHARQ_001475"
     CDS             13065..14876
                     /gene="scm"
                     /locus_tag="ACHARQ_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8409893.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15031..16017)
                     /locus_tag="ACHARQ_001476"
     CDS             complement(15031..16017)
                     /locus_tag="ACHARQ_001476"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8409894.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16040..16606)
                     /locus_tag="ACHARQ_001477"
     CDS             complement(16040..16606)
                     /locus_tag="ACHARQ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8409895.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16620..17036)
                     /locus_tag="ACHARQ_001478"
     CDS             complement(16620..17036)
                     /locus_tag="ACHARQ_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8409896.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17493..19427)
                     /locus_tag="ACHARQ_001479"
     CDS             complement(17493..19427)
                     /locus_tag="ACHARQ_001479"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8409897.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         5589 a         4252 c         3781 g         5900 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    14461 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    14521 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    14581 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    14641 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    14701 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    14761 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    14821 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    14881 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    14941 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    15001 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    15061 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    15121 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    15181 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    15241 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    15301 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    15361 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    15421 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    15481 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    15541 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    15601 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    15661 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    15721 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    15781 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    15841 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    15901 gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    15961 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    16021 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    16081 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    16141 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    16201 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    16261 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    16321 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    16381 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctc
    16441 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    16501 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    16561 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    16621 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    16681 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    16741 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    16801 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    16861 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    16921 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    16981 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    17041 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    17101 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    17161 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    17221 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    17281 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    17341 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    17401 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    17461 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    17521 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    17581 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt
    17641 acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg
    17701 catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc
    17761 atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt
    17821 gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc
    17881 aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca
    17941 gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc
    18001 catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt
    18061 ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa
    18121 ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct
    18181 cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata
    18241 taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata
    18301 gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca
    18361 atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc
    18421 gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc
    18481 ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt
    18541 ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct
    18601 gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt
    18661 gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg
    18721 attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca
    18781 gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg
    18841 atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc
    18901 ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta
    18961 cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga
    19021 atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta
    19081 ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct
    19141 aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt
    19201 tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct
    19261 acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg
    19321 atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa
    19381 acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc
    19441 cttatagact atctatataa tgaatatatg ataattcttt tagtttgata cttattacac
    19501 aatctccata tattatatag at
//
LOCUS       DAYYAG010000049        18504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000049 DAYYAG010000000
VERSION     DAYYAG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.049"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHARQ_001480"
     CDS             99..746
                     /locus_tag="ACHARQ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8409898.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHARQ_001481"
     CDS             1139..3784
                     /locus_tag="ACHARQ_001481"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8409899.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHARQ_001482"
     CDS             4094..5407
                     /locus_tag="ACHARQ_001482"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8409900.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHARQ_001483"
     CDS             5397..6050
                     /locus_tag="ACHARQ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8409901.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHARQ_001484"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHARQ_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8409902.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHARQ_001485"
     CDS             6947..7147
                     /locus_tag="ACHARQ_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8409903.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHARQ_001486"
     CDS             complement(7401..9080)
                     /locus_tag="ACHARQ_001486"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8409904.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHARQ_001487"
     CDS             complement(9082..9294)
                     /locus_tag="ACHARQ_001487"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8409905.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHARQ_001488"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHARQ_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8409906.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHARQ_001489"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHARQ_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8409907.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACHARQ_001490"
     CDS             13269..13466
                     /locus_tag="ACHARQ_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409908.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACHARQ_001491"
     CDS             13619..14104
                     /locus_tag="ACHARQ_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8409909.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHARQ_001492"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHARQ_001492"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8409910.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACHARQ_001493"
     CDS             14981..15508
                     /locus_tag="ACHARQ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409911.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACHARQ_001494"
     CDS             15599..16336
                     /locus_tag="ACHARQ_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8409912.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACHARQ_001495"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACHARQ_001495"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8409913.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACHARQ_001496"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACHARQ_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8409914.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5983 a         3200 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAYYAG010000050        17974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000050 DAYYAG010000000
VERSION     DAYYAG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.050"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1412
                     /locus_tag="ACHARQ_001497"
     CDS             72..1412
                     /locus_tag="ACHARQ_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8409915.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1630..2073
                     /locus_tag="ACHARQ_001498"
     CDS             1630..2073
                     /locus_tag="ACHARQ_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8409916.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="ACHARQ_001499"
     CDS             complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="ACHARQ_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8409917.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4398..5621)
                     /locus_tag="ACHARQ_001500"
     CDS             complement(4398..5621)
                     /locus_tag="ACHARQ_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8409918.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5652..6491)
                     /locus_tag="ACHARQ_001501"
     CDS             complement(5652..6491)
                     /locus_tag="ACHARQ_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8409919.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6508..7188)
                     /locus_tag="ACHARQ_001502"
     CDS             complement(6508..7188)
                     /locus_tag="ACHARQ_001502"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8409920.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7190..8263)
                     /locus_tag="ACHARQ_001503"
     CDS             complement(7190..8263)
                     /locus_tag="ACHARQ_001503"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8409921.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8319..8557)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8600..8833)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8910..9581)
                     /locus_tag="ACHARQ_001504"
     CDS             complement(8910..9581)
                     /locus_tag="ACHARQ_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8409922.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9668..12316)
                     /gene="mgtA"
                     /locus_tag="ACHARQ_001505"
     CDS             complement(9668..12316)
                     /gene="mgtA"
                     /locus_tag="ACHARQ_001505"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8409923.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12658..12832)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13005..13442
                     /gene="ndk"
                     /locus_tag="ACHARQ_001506"
     CDS             13005..13442
                     /gene="ndk"
                     /locus_tag="ACHARQ_001506"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8409924.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13464..14330)
                     /locus_tag="ACHARQ_001507"
     CDS             complement(13464..14330)
                     /locus_tag="ACHARQ_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8409925.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14490..16583)
                     /locus_tag="ACHARQ_001508"
     CDS             complement(14490..16583)
                     /locus_tag="ACHARQ_001508"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8409926.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17332..17955)
                     /locus_tag="ACHARQ_001509"
     CDS             complement(17332..17955)
                     /locus_tag="ACHARQ_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409927.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         5168 a         3722 c         3167 g         5917 t
ORIGIN      
        1 gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa tttaaaagaa
       61 agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca
      121 tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg
      181 ggatcgccgg cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg
      241 tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg
      301 gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt
      361 ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt
      421 ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca
      481 tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa
      541 tcagtgtcat tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg
      601 gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg
      661 gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac
      721 tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca
      781 aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc
      841 tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa
      901 aaatgaatat cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct
      961 catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc
     1021 gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg
     1081 ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca
     1141 aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta
     1201 tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca
     1261 gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca
     1321 ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa
     1381 agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat
     1441 gtggaataga tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt
     1501 tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa
     1561 aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt
     1621 ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt
     1681 atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca
     1741 cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg
     1801 tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt
     1861 tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg
     1921 aaggccatga ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa
     1981 gatcctgaag taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac
     2041 aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag
     2101 gatgttccag aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga
     2161 ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2221 cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt
     2281 ttattaagag gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa
     2341 tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg
     2401 tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa
     2461 tggctttcca catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc
     2521 gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat
     2581 tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt
     2641 ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg
     2701 aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt
     2761 ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca
     2821 gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca
     2881 gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa
     2941 atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag
     3001 ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc
     3061 atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt
     3121 gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga
     3181 ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc
     3241 tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta
     3301 aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt
     3361 gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac
     3421 atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt
     3481 gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca
     3541 accagcgctg gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt
     3601 gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca
     3661 gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg
     3721 atccctgctt gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg
     3781 atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt
     3841 tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag
     3901 acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt
     3961 gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt
     4021 cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct
     4081 cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc
     4141 ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc
     4201 atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac
     4261 acctaaccgc tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt
     4321 tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca
     4381 cacaagttta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct
     4441 tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta
     4501 ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca
     4561 gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg
     4621 attgttggta tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt
     4681 tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca
     4741 tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa
     4801 gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta
     4861 tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg
     4921 acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat
     4981 tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact
     5041 cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga
     5101 tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct
     5161 acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg
     5221 cccccttctt cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat
     5281 tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca
     5341 tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta
     5401 ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa
     5461 aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt
     5521 acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg
     5581 attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct
     5641 agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat
     5701 gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac
     5761 gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg
     5821 tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct
     5881 acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca
     5941 tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct
     6001 tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg
     6061 ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca
     6121 tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact
     6181 aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc
     6241 aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg
     6301 tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta
     6361 tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt
     6421 ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata
     6481 atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact
     6541 gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc
     6601 tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac
     6661 tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat
     6721 gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc
     6781 catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg
     6841 acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat
     6901 cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg
     6961 gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag
     7021 taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc
     7081 aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc
     7141 cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc
     7201 cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac
     7261 cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa
     7321 cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg
     7381 ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa
     7441 tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa
     7501 tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt
     7561 cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt
     7621 ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga
     7681 cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg
     7741 ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca
     7801 aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag
     7861 atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct
     7921 gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt
     7981 ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt
     8041 tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct
     8101 gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc
     8161 cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt
     8221 ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc
     8281 taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg
     8341 ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc
     8401 tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg
     8461 atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa
     8521 ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta
     8581 ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt
     8641 ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa
     8701 cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct
     8761 tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt
     8821 tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact gaaaagtcaa
     8881 gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt taacatttca
     8941 ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt ttcttcgacg
     9001 gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa
     9061 gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc ttctaatcct
     9121 gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga cacttcgtct
     9181 ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat atctgttgct
     9241 cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt taagtctgcc
     9301 aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa
     9361 tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc tgctttcact
     9421 aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat ttcttttggg
     9481 atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt tttttctgtg
     9541 tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt aatcctttcc
     9601 aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa agaaccagcc
     9661 ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca gctgtcacaa
     9721 gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca ggaagtggca
     9781 tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca ataattccta
     9841 atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt actttcggtg
     9901 tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac catcctgcat
     9961 ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa cttccgccca
    10021 ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca aatatcgaac
    10081 ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat ttttttggct
    10141 cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa acatcataga
    10201 tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg aacgtacttg
    10261 caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg atatatttca
    10321 tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt
    10381 tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta tctacagaaa
    10441 taccgatatc agccactttc attgcaggag cgtcattgat accatctcct aaaaatccga
    10501 cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt
    10561 ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc atttccgaga
    10621 ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac actgaacgtg
    10681 taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct ttcagagctt
    10741 tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa
    10801 ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg gtcttttgtg
    10861 cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca agtacggctt
    10921 cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat ttagaaatgt
    10981 tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct ggatcagcaa
    11041 ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact ttttgataat
    11101 tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca tctaacaaat
    11161 ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca cgatcatctt
    11221 catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc aatgtccccg
    11281 ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc aaatttttga
    11341 tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc gtaacgatca
    11401 ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga aaagcttcta
    11461 gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc aatgacataa
    11521 atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat tgaattggtt
    11581 tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca gaaacaatga
    11641 ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt tcgtaacttg
    11701 tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga tagctttccc
    11761 ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc atatccgctg
    11821 gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct tccataggaa
    11881 tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt gatttcagtt
    11941 tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg ctgacgataa
    12001 ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct gctaatacat
    12061 attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat ggcgtgatgt
    12121 atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc gcattttcac
    12181 cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct gatgcatgga
    12241 atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat
    12301 ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt tctcgctgct
    12361 tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc tgagaacaga
    12421 tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg tcatccattt
    12481 gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt ttcgaatagt
    12541 tatttttcca attagaccac ctccttttta tttttcaccg atggggattt gcggcaacaa
    12601 aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct
    12661 cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc tgcttttagc
    12721 taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt
    12781 ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta ttcggttttc
    12841 cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac
    12901 tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt ctcattttcc
    12961 acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa agaacacttg
    13021 ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc caacgttttg
    13081 aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct gaattagcaa
    13141 aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc gattacatga
    13201 cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta
    13261 agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga ggagactacg
    13321 cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct gcggtaaaag
    13381 agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt
    13441 gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag cagttttttc
    13501 tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa gaattcttgg
    13561 tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata ataatctgcc
    13621 atcaatgttt catagatcac tgtacttctt tctttatgtg ataagctccg tttcatatct
    13681 tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga agtcaggttc
    13741 attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg tcgcattctt
    13801 tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca aggaaaatca
    13861 gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta ttcttcaatg
    13921 attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt tttttcgacc
    13981 atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg cctacggtta
    14041 ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag cattcgtctt
    14101 tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata ttctgcaagt
    14161 gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt tttcactgaa
    14221 tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa ttgggactgt
    14281 gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat aatagttctc
    14341 ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg aaaaatacac
    14401 gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca ttagaatgtt
    14461 gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg ttcaacgtct
    14521 gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga aaagacactt
    14581 gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc atttttatag
    14641 tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg caagagattt
    14701 tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg atctttggag
    14761 atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg attattgtaa
    14821 gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg aaaagcaact
    14881 acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc tttatcatcg
    14941 attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta ttcatgattg
    15001 actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg catctgtgcg
    15061 ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa atctttatta
    15121 tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt ttggtaaata
    15181 ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata atgtgccgtt
    15241 cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc gaaattcttg
    15301 tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta aaacggctgt
    15361 tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa tatcccataa
    15421 cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc ataacctaag
    15481 tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa aacagcgctt
    15541 gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt gtctgaccca
    15601 agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa catattttca
    15661 gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct gatcgttgct
    15721 tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac cttcatatta
    15781 atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc tttagcgatc
    15841 ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa aacttcatca
    15901 atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca
    15961 taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc aaatgttcgc
    16021 tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa aataagccct
    16081 gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt gataggtatt
    16141 ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag ccagtagatg
    16201 acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga cactttcgaa
    16261 aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa aataatattc
    16321 aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa cgctggcttt
    16381 ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat tgggttgatc
    16441 cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc atagtaggca
    16501 atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg
    16561 cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt tttcttattt
    16621 tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt tttattcttt
    16681 ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac
    16741 taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca aaagccagtt
    16801 tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc tccttatttc
    16861 ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct gccaactcct
    16921 cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt
    16981 ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc
    17041 ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac ggtgttcaaa
    17101 agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct attttttgaa
    17161 attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc
    17221 aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg
    17281 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc
    17341 ataggattga tattgtcctt gtgatgactc attcgtaata attgaggctt gattttcttc
    17401 acttcccgtt atcgtttgtc cactgccaga atctgaagga cttgttcctg tcgactcgtt
    17461 tttttgagaa gagacagttg ttttcttgac ttgttcattt gtctttttcc attccgctaa
    17521 acgatcggcg accacttttt tgtctacttt ttcagaataa gcggtgatca cttttccatt
    17581 tttcaaacgg gctactttgt cgccatcatg agtcaaaagc aaaacggtca aatgcttgtc
    17641 tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat taataaattg
    17701 attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt aattggcagt
    17761 tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat ctactgattc
    17821 tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca tttttcctga
    17881 aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg
    17941 tctttttttc ctcatgcttt cctccactcc tatt
//
LOCUS       DAYYAG010000051        17963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000051 DAYYAG010000000
VERSION     DAYYAG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.051"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..1592)
                     /gene="abc-f"
                     /locus_tag="ACHARQ_001510"
     CDS             complement(9..1592)
                     /gene="abc-f"
                     /locus_tag="ACHARQ_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8409928.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1697..1825)
                     /locus_tag="ACHARQ_001511"
     CDS             complement(1697..1825)
                     /locus_tag="ACHARQ_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409929.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2170..2313)
                     /locus_tag="ACHARQ_001512"
     CDS             complement(2170..2313)
                     /locus_tag="ACHARQ_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8409930.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2536..3015)
                     /locus_tag="ACHARQ_001513"
     CDS             complement(2536..3015)
                     /locus_tag="ACHARQ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8409931.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3088..3372)
                     /locus_tag="ACHARQ_001514"
     CDS             complement(3088..3372)
                     /locus_tag="ACHARQ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409932.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3450..3644)
                     /gene="ssrS"
                     /locus_tag="ACHARQ_001515"
     ncRNA           complement(3450..3644)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHARQ_001515"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3716..5002)
                     /locus_tag="ACHARQ_001516"
     CDS             complement(3716..5002)
                     /locus_tag="ACHARQ_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8409933.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5140..5811
                     /locus_tag="ACHARQ_001517"
     CDS             5140..5811
                     /locus_tag="ACHARQ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8409934.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5777..6265
                     /locus_tag="ACHARQ_001518"
     CDS             5777..6265
                     /locus_tag="ACHARQ_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8409935.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6278..7225
                     /gene="prmA"
                     /locus_tag="ACHARQ_001519"
     CDS             6278..7225
                     /gene="prmA"
                     /locus_tag="ACHARQ_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8409936.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7226..7990
                     /locus_tag="ACHARQ_001520"
     CDS             7226..7990
                     /locus_tag="ACHARQ_001520"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8409937.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8223..10436
                     /locus_tag="ACHARQ_001521"
     CDS             8223..10436
                     /locus_tag="ACHARQ_001521"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8409938.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10447..10893
                     /gene="dtd"
                     /locus_tag="ACHARQ_001522"
     CDS             10447..10893
                     /gene="dtd"
                     /locus_tag="ACHARQ_001522"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8409939.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11064..12323
                     /locus_tag="ACHARQ_001523"
     CDS             11064..12323
                     /locus_tag="ACHARQ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8409940.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12412..13359)
                     /locus_tag="ACHARQ_001524"
     CDS             complement(12412..13359)
                     /locus_tag="ACHARQ_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8409941.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13753..15045
                     /gene="hisS"
                     /locus_tag="ACHARQ_001525"
     CDS             13753..15045
                     /gene="hisS"
                     /locus_tag="ACHARQ_001525"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8409942.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15060..16829
                     /gene="aspS"
                     /locus_tag="ACHARQ_001526"
     CDS             15060..16829
                     /gene="aspS"
                     /locus_tag="ACHARQ_001526"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8409943.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16938..17813
                     /locus_tag="ACHARQ_001527"
     CDS             16938..17813
                     /locus_tag="ACHARQ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8409944.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         5676 a         3342 c         3837 g         5108 t
ORIGIN      
        1 aaaacgtcct agtcaatcag ttggccgagc ggctggcggc tgatctgctc ctgatcaata
       61 acgagtgctt cttggaagtg ttgctgcaaa aagtagcggt catggctgac tgctaggagc
      121 gttccttgat agtcttgcag catttcttca atttcttctc gagcatagct gtcgaggtga
      181 ttggttggtt catccaaaat caggacgttg attttttcta aaaagagttt cagcaacgcc
      241 aaacggattt tttcaccacc ggataaatct ttgatccgtt tacccacatc gctttgataa
      301 aagccacttt tggctaattg ccgtcgagcg acttcttcgt ttccagtcaa tgtgatggcg
      361 taagccagca aacggctatc gggatcggca aactgcagct gctgcggcaa ataaccgatt
      421 tgcaagctac tacctaattt gatggtgcca ccgtaaggct tttcctcacc gagtaataat
      481 ttgagtaagg ttgattttcc tgcaccattg tcgccgatga tggctaaatg atctccacga
      541 tagactgcaa aactgctgtc tttgaatagt acttgatcag cgtactgctg gtgcaagttt
      601 tgaatgatga aaacttcttt gccagatttt ccaccattag acaagctttg cagccgattt
      661 ttcgggggag gtggcggttt gacgagtgtc agtttcgcca ggcggcgttc cagttctttg
      721 gcttttttga agaaggactc attgtctcct tcatgtgccc actgacgaaa acgacgaatc
      781 atgactttca accgctggac ttctttttgc tgcagttcgt aattttttgt cagctcagcc
      841 aagcgagctt ctttcaagac tcgaaaacgg ctgtagtttc cgggatactc aatcaatgca
      901 ccttcttcca gttcccatgt ttttgtggtg acttgatcga gaaattgccg atcatgagaa
      961 acgaccagaa ccgatttttt tgtattctgt aaatagtttt ccaaccagcg aatactgtta
     1021 agatctaaat gattggtcgg ttcatcgagt aaaagtaaat cggcatcggc tgctaaaatt
     1081 ttggctaaag cgactttaac tcgttcgcct ccgctaagtt ctgttaaccg tgcttcctga
     1141 cggtgtggaa tccctaagcc ttgcaagatc gctgcaatcc ggtcttctaa tacgtaaccg
     1201 cctagttctt caaagcgatg ctgcagctca ccgtaacggg ccagacactt ctctaagttt
     1261 gtatctggtt ctgtcatcaa tgcttcgtag cgactcattt gctgtttgag gtcctgtatc
     1321 tcagcggcag aatggagcaa gtagtggttg acggtcgctt cggaaactgt aagttcttgt
     1381 tcgaccgtcc cgatctttaa gccttttttg cgactgataa caccgctgtc gacaccttct
     1441 ctgccggtca agatttgtaa aaaggttgat ttgccagttc cgttgatacc gactaagaca
     1501 attttttcac cagcttcaat ttgacaggat aaatcctcaa ataatggatt cccgccgatt
     1561 gtttttttaa tagaaattgc ttgtatgatc atgatctctc tccttgagac cactcaaaaa
     1621 ggccatggtt atccatggcc tgtgttattt aaactcagat tatcatgaga aaaatacctt
     1681 gagatggcca ttgattttac gtgaattcaa ctgttttgga caaacttgtc ccgtcaacag
     1741 caactgtaaa accaattgca tggctaaatg cgatatggta tcgttggtaa ttttcattac
     1801 ttgttatttt agtcatgcgc atcatagtat tttccctcct tcgtgtagtt gtcttttcca
     1861 gtgtacctga aagtgtcaga aagttcaact agcgctgaca tttacttaat atttatcgat
     1921 gtgtaattct gtcagagagc tttttttatc tgactgaggg taatgtctcg tggaccacat
     1981 cattgtattc tgagctaaac cagcgttcag gcgaaatcat atggtgtttg ataaaaaact
     2041 ttgtctacat gacttccttt tgccatttgc ttaataccgt tgcattaaac agctcttctc
     2101 tttggaaatc tcaccaacat cgcgttcagg agtatcgttg tggttgcaat ggcaagcggt
     2161 agcctttcat caattcttga atgatttacc gaatgtagca cttgccttct cgcggtaccc
     2221 gaacccgaaa taaaatttta gtttggcgat aaaatcggta tccgccatcg atacttcata
     2281 ttttttcgta tcagattcat cggaaacttc cacattgacg aaccaataag ctttggcatg
     2341 cttaggtcgt tttaaaccac accagggcga ctaatcgccc tggtgttcac ttttttttat
     2401 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
     2461 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
     2521 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
     2581 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
     2641 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
     2701 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
     2761 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
     2821 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
     2881 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
     2941 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
     3001 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
     3061 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
     3121 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
     3181 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagctcc
     3241 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
     3301 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
     3361 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
     3421 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
     3481 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
     3541 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
     3601 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
     3661 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
     3721 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
     3781 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
     3841 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
     3901 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
     3961 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
     4021 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
     4081 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
     4141 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
     4201 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
     4261 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
     4321 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
     4381 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
     4441 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
     4501 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
     4561 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
     4621 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
     4681 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
     4741 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
     4801 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
     4861 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
     4921 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
     4981 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
     5041 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
     5101 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
     5161 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
     5221 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
     5281 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
     5341 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
     5401 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
     5461 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
     5521 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
     5581 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
     5641 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
     5701 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
     5761 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
     5821 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
     5881 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
     5941 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
     6001 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
     6061 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
     6121 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
     6181 ctgggataat cagcaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
     6241 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
     6301 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
     6361 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
     6421 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
     6481 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
     6541 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
     6601 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
     6661 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
     6721 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
     6781 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
     6841 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
     6901 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
     6961 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
     7021 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
     7081 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
     7141 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
     7201 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
     7261 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
     7321 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
     7381 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
     7441 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
     7501 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
     7561 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
     7621 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
     7681 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
     7741 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
     7801 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
     7861 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
     7921 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
     7981 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
     8041 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
     8101 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
     8161 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
     8221 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
     8281 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
     8341 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
     8401 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
     8461 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
     8521 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
     8581 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
     8641 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
     8701 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
     8761 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
     8821 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
     8881 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
     8941 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
     9001 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
     9061 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
     9121 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
     9181 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
     9241 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
     9301 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
     9361 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
     9421 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
     9481 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
     9541 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
     9601 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
     9661 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
     9721 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
     9781 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
     9841 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
     9901 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
     9961 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    10021 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    10081 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    10141 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    10201 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    10261 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    10321 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    10381 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    10441 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    10501 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    10561 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    10621 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    10681 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    10741 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    10801 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    10861 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    10921 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    10981 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    11041 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    11101 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    11161 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    11221 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    11281 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    11341 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    11401 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    11461 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    11521 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    11581 gaacgtttac ttctccgatt gatcacataa aaggtgtcga aaatcgcaaa aaatcagaaa
    11641 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    11701 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    11761 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    11821 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    11881 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    11941 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    12001 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    12061 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    12121 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    12181 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    12241 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    12301 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    12361 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    12421 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    12481 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    12541 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    12601 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    12661 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    12721 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    12781 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    12841 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    12901 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    12961 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    13021 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    13081 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    13141 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    13201 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    13261 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    13321 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    13381 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    13441 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    13501 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    13561 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    13621 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    13681 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    13741 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    13801 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    13861 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    13921 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    13981 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    14041 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    14101 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    14161 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    14221 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    14281 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    14341 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    14401 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    14461 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    14521 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    14581 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    14641 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    14701 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    14761 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    14821 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    14881 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    14941 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    15001 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    15061 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    15121 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    15181 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    15241 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    15301 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    15361 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    15421 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    15481 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    15541 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    15601 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    15661 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    15721 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    15781 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    15841 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    15901 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    15961 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    16021 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    16081 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    16141 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    16201 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    16261 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    16321 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    16381 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    16441 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    16501 gttatgaact aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    16561 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    16621 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    16681 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    16741 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    16801 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    16861 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    16921 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    16981 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    17041 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    17101 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    17161 tacctttatt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    17221 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    17281 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    17341 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    17401 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    17461 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    17521 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    17581 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    17641 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    17701 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    17761 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    17821 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    17881 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    17941 aaaaaagcat gtttattttt tta
//
LOCUS       DAYYAG010000052        17846 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000052 DAYYAG010000000
VERSION     DAYYAG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.052"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..947
                     /locus_tag="ACHARQ_001528"
     CDS             135..947
                     /locus_tag="ACHARQ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8409945.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            944..1744
                     /locus_tag="ACHARQ_001529"
     CDS             944..1744
                     /locus_tag="ACHARQ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8409946.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1905..3173
                     /gene="rseP"
                     /locus_tag="ACHARQ_001530"
     CDS             1905..3173
                     /gene="rseP"
                     /locus_tag="ACHARQ_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8409947.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3241..4950
                     /locus_tag="ACHARQ_001531"
     CDS             3241..4950
                     /locus_tag="ACHARQ_001531"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8409948.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5162..9514
                     /locus_tag="ACHARQ_001532"
     CDS             5162..9514
                     /locus_tag="ACHARQ_001532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8409949.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9658..10131
                     /gene="rimP"
                     /locus_tag="ACHARQ_001533"
     CDS             9658..10131
                     /gene="rimP"
                     /locus_tag="ACHARQ_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8409950.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10155..11330
                     /gene="nusA"
                     /locus_tag="ACHARQ_001534"
     CDS             10155..11330
                     /gene="nusA"
                     /locus_tag="ACHARQ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8409951.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11352..11645
                     /locus_tag="ACHARQ_001535"
     CDS             11352..11645
                     /locus_tag="ACHARQ_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8409952.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11642..11953
                     /locus_tag="ACHARQ_001536"
     CDS             11642..11953
                     /locus_tag="ACHARQ_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8409953.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11966..14272
                     /gene="infB"
                     /locus_tag="ACHARQ_001537"
     CDS             11966..14272
                     /gene="infB"
                     /locus_tag="ACHARQ_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8409954.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14299..14646
                     /gene="rbfA"
                     /locus_tag="ACHARQ_001538"
     CDS             14299..14646
                     /gene="rbfA"
                     /locus_tag="ACHARQ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8409955.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14754..15258)
                     /locus_tag="ACHARQ_001539"
                     /pseudo
     CDS             complement(14754..15258)
                     /locus_tag="ACHARQ_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15423..17786
                     /locus_tag="ACHARQ_001540"
     CDS             15423..17786
                     /locus_tag="ACHARQ_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8409956.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6101 a         3154 c         3920 g         4671 t
ORIGIN      
        1 tttatttaaa aattaaaaaa agaaaaaatt attcagtttt taaataaaaa aacaatgatt
       61 tcataggaaa aacagttaga tattgctata atacttaaga tagtgggaat cttttaagta
      121 taggaggaaa aagaatgtta cgtttttttc cgcaaaaaaa caaatatatt caagaaaaaa
      181 gcgaatttca atttaatacc gatggaccaa ttcctcagca cattgccatc atcatggacg
      241 gcaatggtcg ttgggcgcaa aaccgtcgtt tgccacgagt tgctggccat aaagaaggca
      301 tggaaacagt aaagaaagtc acaaaaaaag catcaaggct tggagtgaaa gtgttgacgc
      361 tttatgcctt ttcgactgaa aactggaagc gaccaaaaga tgaagtaagt tttctcatgc
      421 aactacctgt cgacttcttt gatacgttcg taccagaatt gattaaagaa aatgtcaaag
      481 ttcatgtcat gggttatgaa aacgtattgc cagaacatac gcaagatgct gtaagacgtg
      541 caattgagca aacaaaaaac aatactggaa tggtattgaa ttttgctttg aattatggaa
      601 gtagagcaga gatcgtgact gctgtccaag aaattgccga agaagtagca aaaggagaaa
      661 tacacgcaga agaaatcgat gatgaattga ttgctaagca tttaatgaca ggtttcttgc
      721 ctaaagagtt acaagatcct gagctgatga tccgtacaag tggagaagag cgaattagta
      781 attttctgtt atggcagatc gcatatagtg agctgtattt tacaaaagcg ctttggcctg
      841 attttgatgg tgcgcatctt gaagaagcaa tcgcctctta tcaaaatcga gaccgtcgat
      901 tcggcggtgt aaaaaaaaca gaagatgaga aaggggatca gtcatgaaac aacgtgtgat
      961 cacagcagct gtggcgctag tcttgtttat tccgattata tggatcggag gcatagcggt
     1021 tgagcttaca gcagcaatct tagcggtagt aggagtttac gagctgttcc ggatgaaagg
     1081 attgacactc ttaagttttg aaggagtttt atccgccgtt ggtgcagtgt tacttgtttt
     1141 gccaaaagaa agatggtttt tctttcttcc agagaatgta agtacattta tgttatttta
     1201 tcttatagtt atgattcttt taggaacatc agttatttct aaaaatacgt atacgattga
     1261 tgaagcaggt tttcctgtgc ttgtcagctt gtatgtggga ataggtttcc agaattttgt
     1321 caatgcacga acagaaagct tggctgtttt gatatttggt ctatttattg tttgggcaac
     1381 tgatatcgga gcatatatgg ttggtcgacg ttatggtcag cataagttaa gtcctgatat
     1441 ttcgcccaac aaaacgattg aaggtgcgct cggtggtatc ggtagtgctg ttgtagtcgc
     1501 atttctttat ttattgctat acccagcaaa agaactattc aatcatggta tgtttgtcat
     1561 gcttctgatg acgattattt tttcagcagt cggacaattt ggtgatttgg tcgaatctgc
     1621 aatcaaacgt cattatgatg tgaaagactc gggaaatatc ttaccaggcc atggtggaat
     1681 cttagaccgt ttcgacagtt tgctgtttgt atttcctatc atgcatttat ttggcattat
     1741 ttagtaagta tacctgcgtt tctttgtgaa tttcataaaa gacgcaggtt tttatttttc
     1801 attatattga tactattggt gtctttggta tactatggta aaattatagt gttttatcgt
     1861 tgttttataa aaaacacaaa aaacaagtaa aggagaaaaa agctatgaaa acgattctga
     1921 catttatcat cgtttttggt atattagtga ttgttcatga gtttggtcat ttcttctttg
     1981 cgaaacgatc aggaatcctc gtccgcgaat ttgctatcgg tatgggtccg aaaatttatg
     2041 ggcatcaagc aaaagacggg acaacatata cgcttcgcct attacctatt ggcggttatg
     2101 ttcgaatggc aggcaatggg gatgatgaaa cagaaatggc accaggaatg cctctttctc
     2161 ttttactaaa ttctgacggc atcgtagaga agataaattt aagtaagaaa atccaattga
     2221 caaatgctat cccgatggaa ttgagccgtt atgatttaga agatgagctg actatcactg
     2281 gttatgtgaa tggagacgaa acagaagtag taacctaccc tgttgatcac gatgcgacga
     2341 ttattgaaaa cgacggaaca gaaatcagaa ttgcgccaaa agacgtacag ttccaatcgg
     2401 ctaaattgtg gcagcgtatg ctgaccaact ttgctggacc catgaataac tttattttgg
     2461 caatcgtgtt attcatcatt ctggcattta tgcaaggtgg tgttcaagtg acgaacacta
     2521 atcgcgtagg agagatcatg cctaatggag ctgcggcaga agcaggctta aaagaaaatg
     2581 atgaggtagt cagtgtggac ggaaaagaaa tccattcatg gaatgatctg acaacagtca
     2641 ttacgaaaaa tccaggtaaa acattagact ttaagatcga acgagaaggt caggtgcaat
     2701 ctgtcgacgt gacaccgaaa tctgtagagt caaatggcga aaaagttggc caacttggta
     2761 taaaagcccc aatgaataca ggttttatgg ataagatcat tggagggacg cgtcaagctt
     2821 tcagcggttc attggaaatt ttcaaagcct taggatcact atttacaggt ttcagtttag
     2881 ataaattagg cggacctgtc atgatgtacc aattgtcttc agaagcagca aatcaaggtg
     2941 taacaactgt tattagtttg atggcgcttc tttcaatgaa tctcggaatc gtcaatctgc
     3001 ttccgattcc tgccttagat ggcgggaaat tagtcttgaa tatttttgaa gggattcgcg
     3061 ggaaaccatt aagtcaagaa aaagaaggaa tattgacatt agctggcttt ggatttttaa
     3121 tgttgttgat ggtgctagtt acatggaacg atattcaacg attctttttc tagaaaaagt
     3181 tgacaaatag ggtataataa caaacgataa ctattttgag gaaataaagg agtatataga
     3241 atgagacaat caaaaatgtt gattccaaca ttacgcgaag ttccaaatga tgcagaagta
     3301 ttaagccatc aaatcttgct aagagctgga tatatccgcc aagtatcagc gggtatttat
     3361 tcatatctgc cattagcaaa tcgtgtattg gaaaaactga aacgcattat gcgggaagaa
     3421 tttgaaaaaa tcggagcggt agaaatgctg atgcctgcca tcttgccagc agaactttgg
     3481 gaagaatccg ggcgttacga aacatatgga ccaaacttgt atcgtttcaa agaccgcaat
     3541 gatcgaaaaa tgatccttgg accaactcac gaagaaactt ttactgattt gatgcgtaat
     3601 gaaatcaatt catataagaa attaccttta aatctttatc aaatccaacc taaatatcgt
     3661 gacgaaaaac gacctcgctt cggtttgctt cgcggaagag agtttatcat gaaagatgca
     3721 tattctttcc atgcaacgga agaaagtttg gacgagacat ataaagatta cgaaaaagcc
     3781 tataccgaag tattcaagcg ttgtggctta gaatttcgct cgatcatcgg agacggcggc
     3841 gcaatgggcg ggaaagactc aaaggaattc atggcgatct cagaaatcgg ggaggatacg
     3901 atctgttatt cgactgaaag tgattatgct gcaaacttgg agatggctac aagctactat
     3961 gtatcaaaaa aatcgcatga aacacagctt gaattggaga aagtagctac acctaacgta
     4021 aaatcaattg acgaagtagc agatttcttt tctgttgaac ctcaaaaaat catcaagtct
     4081 gtattgttca ttgcggatga acaacctgtc cttgttttgg tacgtggaga ccatgaagtc
     4141 aatgacgtaa aactgaaaaa ctttttatct gctgactttt tagaagaagc aacagaagaa
     4201 gaagcacaaa aatatctagg tgctgagttt ggttctgtcg gaccagtcgg tgtttcagat
     4261 gacgtaaaag tatatgcaga ccgttatgtc caagatttag cgaatgccat tactggagcg
     4321 aacgaaacag gattccacta taaaaatgtg aatccaaaac gcgatttcac tgtgctttct
     4381 tatgaagatc ttcgttttgt acaagaagga gatccttcac cagacaacaa cggtgttctt
     4441 gcctttactc gaggaatcga aatcggacat atattcaaaa tcggcactcg ttacagtgaa
     4501 tctatgggag caactgtact agacgaaaat ggacgagaaa aatttgtgat tatgggctgt
     4561 tatggtatcg gtgtaagtcg tttattatca gcaatcgtag aacaacattc agatgaacaa
     4621 ggaatcaatt ggccaaaagg aattgcgcca tttgatctgc atatcgtcca aatgaatcta
     4681 aaagacgaat accaaacaag cctgacgaat gaactagaag aagcgatgac aaaagctggc
     4741 tatgaagtac tggttgatga cagaaatgaa cgcgcaggag tgaaattcgc ggattctgat
     4801 ttgattggct gtccaatcag aatcactgtc ggtaaaaaag cagtggatgg aatcgtagaa
     4861 gtgaaaatca agaaaactgg cgaaatggtt gaagtacgta aagaagaatt ggcgaatact
     4921 ttaccaatcc tcttgaacca aggcgaataa taagcgttaa aacacagaaa ataaaaatag
     4981 ctgaatcagc tgtttttgac aatctgatga ttcgctttta aaattggaac tgcgatttga
     5041 atcgcagttc caatttttta atgtaaaact cagaaaaaac gagtatacta aaaggtactg
     5101 aaaaagaaga ggataagatg cgatcaattg cattgtttct catcagaaag ggcgtatatc
     5161 tttgtcagaa aaacgagatt tatttgaaaa actgttggaa caaatccaac tagaggaagc
     5221 agaaaaaaat catccactgc ttactgctgg tgaaatggat agtgtagtgg tccatcgtaa
     5281 atcgcgttta tgggaattta cgctacattt ttccaaaatt ttgcctatta tgctatatcg
     5341 gtcattgact cagcatctga ctatcgcttt taaagatatt gcacaagtca aactgaatat
     5401 cttagcagat gaccagacat tcgatgaaca gctcttgcaa gattactggg cccaagcttt
     5461 ggaaaatcag caatgcgata caccactagc acagcaagta ttgaaaacgc aggtacctgt
     5521 gataaaagat cgtaaagtca tcttgccaat cgacagtaca ggagctattt cttatttgaa
     5581 gcaacaatac ttgccattag tagaagagtt gttcgtcagt tacggattcc caaaatttcg
     5641 tatcgaacca gaagtggatg agcagcaagc ggaacgtgta ttgaaacttt ttgaagaacg
     5701 caaacaagaa caggcagaag cctttatgaa gcaagcagct gaaagtctag ttgtccacga
     5761 acaaaagaaa aaggaaagaa aagagcaatc gccagctctt gaaggaccca tccagctagg
     5821 acgaaatatt ccagccgatg aaccaacgac accaatgatc agtatcgttg aagaagaacg
     5881 aagagtcaca cttgaggggt acgtattcga caaagaagtc agagaactac gttcaaaacg
     5941 caagatcttg actttgaaaa tcacagatta tacttcatca tttatcgtca aaaaattctc
     6001 gaacggcgaa aaagatgaac aagtatttga tgcgatcagt gtcggtagct ggttaaaggt
     6061 acgaggaagt attcaagaag atacgtttgt ccgtgatttg gtcatgaatg cgcaagatat
     6121 catagaagtc aaacatacac ctcgcaaaga ttatgctcct gaaggggaaa aacgagtaga
     6181 gcttcatgtt catagcaata tgagtacgat ggatgcaaca aacagcatct ctgatcttgt
     6241 ggcacaagca ggaaaatggg gacaccgagc tatcgccatt acagaccatg gtggtgcgca
     6301 agcctttccg gaagcacata gtgctggaaa aaaagcaggt gtgaaaatct tatacggcgt
     6361 ggaagcgaat gtcgtagatg atggtgtccc aattgcttat aatgatgcac atgaagcatt
     6421 gagtgaagcc acatacgtcg tatttgacgt ggaaacgaca ggattgtctg cagtatatga
     6481 cacgatcatc gagctagccg cagtcaaaat gtataaagga aatgtgatcg aaagctttga
     6541 tgaatttatc gatccaggac atccattgtc acgaacaacg gtagatctga ctggtatcac
     6601 ggatgaaatg gttcgagggt caaagtcgga agaagaagta ttgcggatgt tccttgaatt
     6661 ttcaaaagat acaattttgg tagcccacaa cgcggctttt gatatggggt tcttgaatac
     6721 tagctacgca aggtatggta ttcctgaagc ggccaatcca gtcattgata cgttggaatt
     6781 agctagatat ctgtatcctc aattcaaacg tttcggatta ggtgtgttat ctaaaaagtt
     6841 tggtgttagc ttggaacagc atcaccgagc aatctatgat gctgaagcaa ctggtcactt
     6901 agcatggatc tttgtgaaag aggcaatgga caatcataac atgctttatc atgatcagct
     6961 aaatgagcat atcggagaag gagattctta caaaagggct cgacctttcc atgtaacgat
     7021 tttagcaaaa aatcaggcag gactgaaaga tttgttcaaa ctgatttcaa tgtcgaatgt
     7081 cgaatacttc gaacgcgtcc cacgaatacc acgctcccaa ttgaaaaaga tgcgtgaaaa
     7141 tctattgatc ggctcagctt gtgataaggg agaaatattc gaagccatga tgcaaaaagg
     7201 ggtagaagag gcaagaaacc gagcaaaatt ctatgattat attgaagtta tgccaaaagc
     7261 ggtctatgcg cctttgatcg agcaagaatt ggtaaaaaat gaacatgatt tggaagaaat
     7321 tatccaaaat ttggtagaaa tcggaaaaag tttggataaa atcgttgttg caacaggtaa
     7381 tgtacattat ctgaacgaag aagatgccat ttaccgaaaa attttgatca attctatggg
     7441 cggagcgaat ccgttgaatc gtcatagttt gccggatgtc catttccgaa caaccgatga
     7501 gatgctaact gcatttcact ttttagggga agaaacagcg aaagagatcg tagtagaaaa
     7561 tacgaataaa atcgcagata tctgcgaaga agtgattcca gtcaaagatg aactttatac
     7621 accgaagatt ccaggttcag aagatgaaat atcagaattg agttacacaa aagcaaaaca
     7681 aatgtacgga gatccgttac ctgagatcat tcaaaaacga ttagaaaaag agttgaattc
     7741 tattaatggt aatggcttct cggttattta tctgatcgcc caaaaactag tgcataaaag
     7801 taatgaagat ggctatctag taggttcacg tggctctgtt ggctctagct ttgttgcgac
     7861 gatgacagga atcactgaag ttaatccatt agcccctcat tattattgtc cagagtgcca
     7921 atattccgag ttttttgaag atggtacgta tggttctgga tttgacatgc cagaaaaaca
     7981 atgtccaaaa tgtggtgcac gcttaaacaa agacggtcat gatattccat ttgagacatt
     8041 tttaggattc catggagaca aagtgcccga tattgatttg aacttctcag gtgattatca
     8101 agcagaagcc cataactata cgaaagtatt gtttggcgaa gactatgttt accgtgccgg
     8161 aacgattggt acagtcgccg acaaaacagc atatggctat gtaaaaggat acgaaaggga
     8221 caataatctg cagtttcgaa gtgcagaagt cgacagatta gcaaaaggcg ctactggagt
     8281 caaacggaca accggccagc atccaggggg gatcatcgtt attcccgatt atatggatgt
     8341 ctatgatttt acgccgatcc aatatccagc agacgatcaa aattcagaat ggaaaacgac
     8401 tcattttgat ttccactcga ttcacgataa tgtattgaaa ttagatattc tagggcatga
     8461 tgacccaact gttattcgga tgcttcaaga tttatcaggg atcgacccac aaacgatccc
     8521 tacggatgac ccggaagtga tgcggatatt tgccggtcct gaagtacttg gagtaagtca
     8581 ggaacaaatt tattccaaaa cagggacatt aggtattcca gaattcggca ctcgttttgt
     8641 tcgagggatg ttagaagaaa cgcatcctac aacttttgca gaactactgc aaatttccgg
     8701 actatctcat ggtacagacg tatggttagg aaatgcggaa gaattgattc gacgaggaga
     8761 tgcgacactg gcggaagtaa tcggctgtcg tgatgatatt atggtttatt tgatccatgc
     8821 tggattagat agcgggatgg catttaaaat catggaaact gttcgtaaag gacaatggaa
     8881 caagatccct gatgaattgc gagaaaccta tctatcggcg atgaaagaaa ataatgtgcc
     8941 cgactggtat atcgattctt gttcaaaaat caaatatatg tttccgaaag cccatgctgc
     9001 agcttacgta ttgatggctc ttcgtgtcgc ttatttcaag gtttatttcc ctattttgta
     9061 ttactgtgca tacttttctg tacgtgcaga cgactttgat ctagtctcca tgtgcaaagg
     9121 aaaagatgct gtgaaacaag caatgaaaga aatcacggac aagggattag atgcttccgt
     9181 aaaagaaaag aatcaactga cagtgctgga gttagccaat gaaatgctgg aacgaggatt
     9241 caaatttgga atgattgatt tgtataaatc ggatgccgtt aactttgtta ttgaaggtga
     9301 tacgttgatc gcaccattta gagctgtccc aagtttggga accaacgtgg cgaaacaaat
     9361 cgttgaagca agaaaagacg gtccattttt atccaaagaa gatcttgcca cccgcggtaa
     9421 agtttcaaaa acattgatcg aatatatgaa cgacaatggc gtattgaaag atctaccaga
     9481 tgaaaatcaa ttatcgttat tcgatatgct ataagcatct gttttcctgt ctgcttgtaa
     9541 ttttaagcag agtatggtat agttcttata ggtaatgata ctaactgtga gtgagcggat
     9601 ttttcgctca ctctttttaa tggaattacg cacttaattt ttaaggaggc gaaacttttg
     9661 agtagcgtcg ttgaaacagt cacagaaatg gtgacaccga ttttagatga acaaaatttt
     9721 gaacttgtcg aagtcgaatt tgtaaaagaa ggaaaaagct ggttcttacg agtatttatt
     9781 gacaaagaag gcggtatcga tatcgaagaa tgcgcctttg tcagtgagaa actgagtgaa
     9841 aaacttgatg cagcagatcc agatccgatt cctcaagctt atttcctgga ggtatcctcc
     9901 ccgggagcag agcggccatt gaaaaaagaa agcgactatg aaaaggcact tggtgattat
     9961 gtccatgtct ccttatacca accagtagat ggcgaaaaac aatatgaagg atttcttcaa
    10021 tcatttgatg ctgaacagct tacattgaaa atacgcataa aaacacgaga aaaagaattg
    10081 acttttgacc gaaaaaatat tgcaaaagct cgcttagcta tccaatttta atcacggaga
    10141 ggaatcagaa aaaaatgagt aaagaaatgt taaatgcgtt agacgcttta gaagctgaaa
    10201 aagggatctc aaaagaaatc gtgatcgatg ctttagaagc agcattggtt tctgcataca
    10261 aacgtcacta tggccaagca caaaacgtcg aagtggaatt tgatcagaaa aaaggaaaga
    10321 tccatgtcta tgcggtaaaa gaagtaacgg aagaagtgat ggattcacag ttggaagtct
    10381 cattgaaaga tgcactttta atcaatccag catatgagat cggtgatacg atccgttttg
    10441 aagtaacacc aaaagatttt ggacggatcg ctgcgcaaac ggctaaacaa gttattttac
    10501 agcgtgtacg tgaagcagaa agaacgatta tttataacga attcagtgct tatgaaaaag
    10561 acatcatgca aggaatcgtc gaacgccagg ataaacgtta tatttatgtc aatcttggta
    10621 aaattgaggc agttctttct aaacaagatc aaatgccaaa tgaattttat caaccacatg
    10681 atcgaatcaa agtttatgtt tcccgagtag aaaatacgtc taaaggtccg caagtatttg
    10741 ttagccgcag ccatccagac ctattgcgtc gcttatttga acaagaagta ccagaagtct
    10801 atgatggaat cgttgaaatt gtcagcatcg cccgtgaagc tggcgatcgt tcaaaagtgg
    10861 cagttcgctc aaatgatcca aatatcgatg cagtcggtac atgtgtcggg ccaaagggac
    10921 aacgagttca agcaatcgtc aatgaattaa aaggcgaaaa catggatatc gtcgaatgga
    10981 atgaagatcc agctgtattc atcgcaaatg cattgaatcc tgcacaagtc atggatgtga
    11041 tcttcgatga gacaaatcca aaggcctgca cagttgtcgt tccagactac caattatcgc
    11101 tagcaatcgg taaacgcgga caaaatgcac gtttagctgc taaattgact aaccataaaa
    11161 tcgatatcaa atcagaatct gacatgacgg aattttatga aaatcaagaa caacaaaaag
    11221 aaacagaaga actgcatgat gaagcaatcg ttcagtcaga tttaacagac gatgaatatg
    11281 aaacaattgc attcaacaat gaaacggtcg aagaaaaacc agaagcttaa ctttagtaag
    11341 gaggcaaaga gatgaaacaa aggaaaattc ctttacgcaa atccgtggtt tcaggtgaaa
    11401 tgaaacctaa aaaagagatg gtccgaatca cccgttcaaa agaaggggaa gtatcgatcg
    11461 atccaactgg aaaaatgcct ggacgaggag cgtatgtctc acttgagccg gaagaagtac
    11521 aacaagcttg ggataaaaaa attctcgatc gagtactcga aacaacatta acagatgagt
    11581 tttatcaaga acttcttgac tatgtcactc accaaaaagc acgtaaagag ttatttggaa
    11641 aatgaataca gttaatcggc aaaaagccat gaatctcatc ggtctagcga tgcgcgcggg
    11701 aaaactaatc acgggtgaag aactgaccat tgctgaaatc cgcaaacaaa aagcaaaatt
    11761 ggtgtttgtg gcaaccgatg caagtgagaa tacaagaaaa aagataaaag ataagagttc
    11821 atattatgaa gttccttgct ttgagctatt ttctgaagca gaaatcacgc agatgatcgg
    11881 aaaacctcgc aaggtattcg gaataatgga tgccggattt gcgaagaaga ccaaggagct
    11941 aattgaaggt taggaaggtg attgcatggg caacaaaaga atctatgaat tagcaaaaga
    12001 actcaaaaaa tctagtaaag acgtcgtgga aaaagcacaa caacttggca tcgatgtgaa
    12061 aaaccatatg ggtgcgatta ctagcagtga tgagaagaaa ttgcaacaag cgttcaaaaa
    12121 cccaagcacg aaacaacaag ctgcacaaca agcaagcaga aaaccagcca atcaacagcc
    12181 gaacgatcaa cgtaaaaaag aaacaacgaa caatcaacaa aaaaataaaa ataaccgcag
    12241 ttatcaagat cgcggtcaag ggagcggtca agtgaatcaa ggaaaaaaac caacaacaaa
    12301 ccaaaataat acgggaaata ccagcagcca aaatcgtcaa ggaacgagcc aaggcggcaa
    12361 tcaaaaccgt actaacaata acaacaaacc aaacaataac aatcgcggca aatttaataa
    12421 taataaccgt aatcgttata ataaaaaagg taaaaaaggc aaacagcaac aatcaactaa
    12481 accagctgta ccaccacgca aattccgtga attgccagat gtattagagt atactgaagg
    12541 tatgaacgta gcagatattg cgaaaaaaat tcatcgtgag ccagcagaaa tcattaaaaa
    12601 attatttatg cttggtgtca tggtcaatca aaaccaagca ctggataaag atacgattga
    12661 actgttagcg acggactatg gcatggaacc acaagaaaaa gtccaagttg acattgctga
    12721 tatcgataaa ttcttcgagg cagaagaagt cgatccagat aaattagtct cacgtccgcc
    12781 agtagtaacg attatgggac acgtagacca cgggaaaaca accttattgg atacattgcg
    12841 ccattcacgt gtaacaagcg gcgaagccgg cggaatcaca cagcatatcg gtgcctacca
    12901 gatcgatatt gatggtaaac caatcacttt cttggataca ccaggacatg cggcctttac
    12961 aagtatgcgt gcacgtggag caagtatcac agatatcacg atcttagttg ttgctgcaga
    13021 tgatggcgtc atgccacaaa cagttgaagc aatcaaccat gccaaagctg caggcgttcc
    13081 aatcatcgtc gctgtaaata aaattgataa accaggtgca aatcctcagc atgttatgca
    13141 agaattaagt gagtatgaat taattcctga agcatggggt ggagaaacga tttttgttga
    13201 gatctcagcg aaattcggcc aaaacatcga agaattgctg gaaatgatcc tgttagtcgc
    13261 agaagtagaa gatttaaaag cagatccaac acaacgcgca atcggaacag tgatcgaagc
    13321 acgtcttgat aaaggtaaag gtcctgtgtc aaccttactt gtccaagaag gaacacttca
    13381 cgttggtgac ccaatcgttg tcggtaatac ttacggacgt gtccgtgtaa tgacaaacga
    13441 tcttggccgt cgcgataaag aagcaggacc tgcaacgcca gtagaaatca ctggcttgaa
    13501 tgatgtacct caagcaggtg accgtttcgt agtcttcgaa gacgaaaaga cagcacgtgc
    13561 agcgggtgaa gaacgcgcaa aacgtgcatt attggaaaac cgtgctgcca caagccgtgt
    13621 gactttggac aacttatttg aaagcctgaa agaaggcgaa ttgaaagaag ttaacgtaat
    13681 catcaaagca gatgtacaag gttcagcaga agcactggct gcatcattga agaagattga
    13741 tgtggaaggt gtccgtgtaa aaatcgtcca ttcagctgtc ggagcaatca atgaaagtga
    13801 tgtaacctta gcagcagcca gcaacgctat tatcatcggt ttcaacgttc gtcctacacc
    13861 gcaggcaaaa gtgcaagctg atactgaaga agtcgatatt cgtcttcacc gtatcatcta
    13921 taaagccatc gaagaaatcg aaactgcgat gaaagggatg ctagaccctg aatttgaaga
    13981 aaaaatcacc ggtcaaatga tcgttcgtga aaccttcaaa gtttctaaag ttggtacgat
    14041 tgcaggtgcc tatgtaacag aaggatatat tcgtagagat agcggtgtcc gtgtaatccg
    14101 tgacggaatc gtaatctatg aaggccaact tgcaagtctg aaacgattca aagatgacgt
    14161 gaaagaagtc aaaatgggtt atgaatgtgg tgcgatgatt gaaaaattca atgacatcaa
    14221 agtagatgac gtaattgaag gatttatcat ggaagaagtc aaaaatgact aaaaactgat
    14281 aaaaggaggc caatcattat ggctaactac cgtgatcgcc gagtcggtca agaaatctta
    14341 aaagaagtca atgatatttt gcgcaaaaag gtccgtgacc ctcgtgtgga gaacgtaacg
    14401 atcacagatg tgaaagttac gggggactta cagcaagcaa cgatctatta cagcatttta
    14461 tctgacttag catcagataa gaaaaaagca caagatggat tgaataaagc atcaggattg
    14521 attcgtcgag agttagggca caatctaagt atttataaga cacctgaatt gttctttgaa
    14581 cttgatgaat cagttgtcta tgggaaccat atcgatgaat tgcttcgcga cttgaacaaa
    14641 gactaatcaa aaaatcccgc aaatcctgtg aaatcagggg ttgcgggatt tttttgtttt
    14701 cattaagacg tattacctaa gagttccatg actttagtct ggtagcgtgt cagctaacca
    14761 aaaaagagaa aaagaaaagt aaaaaattcg actttctttt ttctatagta tataaatttt
    14821 tgcatataga taaagatcaa gataccgata acggaagcta gattgtaatc tattttagtg
    14881 acagtcaggt atagccaagt aactagaaga tcagtggaac ctgcaccata aaagtttgag
    14941 tttgtcggtc cgttgacagt taaaagaaaa atcacgttga agttattgat atttccgatt
    15001 acgccgcttc gatttggtca cgatccaaat tttggataat ccctgtagaa attagcatag
    15061 tagctgggat accaattcac atattgacaa agataacaga gacttttaca taaaacggat
    15121 caatcaggaa aggaattgct cgatccagcc ccagttttcc aacaatgtgt taactggcct
    15181 agcgtcattc agcaaattat gcatgatgag gagcgaaatg aactgcggaa cagccattgt
    15241 gataacgaca agttttttgg atcaatgtaa aaaacgtcag cgaaaggaaa aatttcgtgg
    15301 ttttttatac ctgttcattt ggctagatat aaaatccaaa caaatgaaca gaagtgctta
    15361 ttaggtttca attgggagta tctgcgctag aataagagta ataatgaaaa ggagtgttat
    15421 gtatggatta ttctagtaaa gaatactttg ataaaatgac ggcttggtgg cgtgctgcta
    15481 attatctttc tgttgggcaa ctttatctta aagataaccc cttactgaga cgtacactga
    15541 aaccagaaga tgtcaaaaaa catccaatcg gtcattgggg aacgattcct ggccaaaatt
    15601 ttatttatgt ccatttgaac cgagtcatca ataaatatga tttgaacatg ttttatatcg
    15661 aagggccagg acacggcgga caagtcatgg tatcgaatgc ttatttagat ggaagttaca
    15721 cagagatcta tccagaagta acggaagatg agacaggaat gcaaaaatta ttcaagcgat
    15781 tttcatttcc tggaggcatt gcttctcatg cagcaccaga aacaccaggt tctatccatg
    15841 aaggcggaga gcttggatac tctctttcac atgcagtcgg agctgtatta gacaatcctg
    15901 aagtaatctc tgctgtcgtg attggtgatg gtgaagcaga aaccggtcct ttagctggaa
    15961 gctggttctc aaatgtcttt atcaatccag tgatagatgg agctgtttta ccgatccttc
    16021 atttaaatgg tgcaaaaata gccaacccaa cgatcttggc acgtaaatca gatggagaat
    16081 tagcaaatta ctttaacgga cttggctggg agcctttctt tattgaagga aatgatccag
    16141 aaaaattaaa tcctgttatg gctgaaaaaa tggatcaagc aatcgagaaa atcaaatcca
    16201 tccaaaaaga agctcgcctg aagacagcaa cagatgtcgt gatgccaaaa tggccagtat
    16261 tgatcgtacg cacaccaaaa ggatggaccg gtccaaaaga atgggacggg gaaccaattg
    16321 aaggaacctt ccgagcacac caagtaccga ttcctgtaga tcaagaacat atggatcatg
    16381 cagatgcttt actccgttgg ctgaaatcct atgaaccaga aaaattattt gatgcacaag
    16441 gacgtatttt agaagaaatc agagaaattg cgcctactgg tgaccaaaga atggcaaaaa
    16501 atcctatcac caatggtgga atcgatccaa gaccgcttat tatgccagat tggaaaaaat
    16561 atactttgca atttgaaaaa ccaggctccg ttaaagcaga agatatgacc gaacttggta
    16621 aattcgttcg cgagatcatt gagaaaaatc cagaaaattt ccgtatattt ggaccagatg
    16681 aaacaaaatc aaaccgattg aatcaagttt tcaaaacgac aaatcgccaa tggatggaaa
    16741 aaatcgaacc agaaaatgat gaatggcttt cgccatctgg tcgcgtcatc gacagtcaat
    16801 tatcggaaca ccaagatgaa gggttcttag aagggtacgt tctgactgga cggcatggtt
    16861 tttttgcaag ttacgaatca ttcttgcgag tggttgactc tatgttgact cagcacttca
    16921 aatggatacg gaaatcacat gacctttctt ggcgaaaaga ttatccttcg ctgaatctca
    16981 ttgcaagctc aacagtcttc cagcaagatc acaatggata tagccaccaa gatccaggaa
    17041 tattgacaca tttggcagaa aagaaggcgg agttcatccg tgaatatctg cctgcggatg
    17101 caaatacatt gctcgcagtc atggataaag cattccgctc gtcagaaaaa atcaatttga
    17161 tcatctcttc caaacatccg cgtgctcaat tttactcagc agaagaagca gccgtcttag
    17221 tgaatgaagg cttgaaaatc attgattggg cttcgacagc aaaagaggag gaacctgaat
    17281 tggtcattgc tgctgcagga acggaatcta acttagaagc attagcagct gtaactttgc
    17341 tgctggaaga attcccaaaa ctaaaaattc ggttcatcaa tgtcgtagat ttattgaaat
    17401 tacgtcatcc aagtcaagat ccgcgtgggt tgagcgatga agaatttgat caatatttta
    17461 caaaagacaa accgatctta tttgctttcc acggttatga aacattggtc cgcacgattt
    17521 tctttgatcg tcataaccat catttaatga ttcatggcta caaagaaaat ggagatatca
    17581 cgacaccatt tgacatgcgt gtagtaaatg aacttgatcg ttatcatttg gcaaaagacg
    17641 cagctttaaa aatcaaagga agtcaagcag aagattttgc ggaaaaaatg gatcagaaat
    17701 tacaagaaca tcaaaactat attcgtgaaa atggtattga tctgccagaa gtattggatt
    17761 ggaaatggaa aaatcttgac caataaaatg ataaaatcga tcccgcaaaa cagaattttt
    17821 tacattgtat tatttgtcaa attaag
//
LOCUS       DAYYAG010000053        17768 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000053 DAYYAG010000000
VERSION     DAYYAG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.053"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..930
                     /locus_tag="ACHARQ_001541"
     CDS             100..930
                     /locus_tag="ACHARQ_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8409957.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1174..1423)
                     /locus_tag="ACHARQ_001542"
                     /pseudo
     CDS             complement(1174..1423)
                     /locus_tag="ACHARQ_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1614..1808)
                     /locus_tag="ACHARQ_001543"
     CDS             complement(1614..1808)
                     /locus_tag="ACHARQ_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409958.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2313..2498)
                     /locus_tag="ACHARQ_001544"
     CDS             complement(2313..2498)
                     /locus_tag="ACHARQ_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409959.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3348..3689)
                     /locus_tag="ACHARQ_001545"
     CDS             complement(3348..3689)
                     /locus_tag="ACHARQ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8409960.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3689..3919)
                     /locus_tag="ACHARQ_001546"
     CDS             complement(3689..3919)
                     /locus_tag="ACHARQ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8409961.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4206..4544
                     /locus_tag="ACHARQ_001547"
     CDS             4206..4544
                     /locus_tag="ACHARQ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409962.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4749..5311)
                     /locus_tag="ACHARQ_001548"
                     /pseudo
     CDS             complement(4749..5311)
                     /locus_tag="ACHARQ_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6043..6624)
                     /locus_tag="ACHARQ_001549"
     CDS             complement(6043..6624)
                     /locus_tag="ACHARQ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8409963.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6807..7433)
                     /locus_tag="ACHARQ_001550"
     CDS             complement(6807..7433)
                     /locus_tag="ACHARQ_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8409964.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7455..8786)
                     /locus_tag="ACHARQ_001551"
     CDS             complement(7455..8786)
                     /locus_tag="ACHARQ_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8409965.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8941..9342)
                     /locus_tag="ACHARQ_001552"
     CDS             complement(8941..9342)
                     /locus_tag="ACHARQ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8409966.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9428..9634)
                     /locus_tag="ACHARQ_001553"
     CDS             complement(9428..9634)
                     /locus_tag="ACHARQ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409967.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9729..11231)
                     /locus_tag="ACHARQ_001554"
     CDS             complement(9729..11231)
                     /locus_tag="ACHARQ_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8409968.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11249..11704)
                     /gene="argR"
                     /locus_tag="ACHARQ_001555"
     CDS             complement(11249..11704)
                     /gene="argR"
                     /locus_tag="ACHARQ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8409969.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11802..12419)
                     /locus_tag="ACHARQ_001556"
     CDS             complement(11802..12419)
                     /locus_tag="ACHARQ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8409970.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12640..14013
                     /locus_tag="ACHARQ_001557"
     CDS             12640..14013
                     /locus_tag="ACHARQ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8409971.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14088..15200
                     /locus_tag="ACHARQ_001558"
     CDS             14088..15200
                     /locus_tag="ACHARQ_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8409972.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15272..16909)
                     /locus_tag="ACHARQ_001559"
     CDS             complement(15272..16909)
                     /locus_tag="ACHARQ_001559"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8409973.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17077..17685)
                     /locus_tag="ACHARQ_001560"
     CDS             complement(17077..17685)
                     /locus_tag="ACHARQ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8409974.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5022 a         3630 c         3112 g         6004 t
ORIGIN      
        1 ataccaatac tttatataat tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca
       61 gaatgagaat gtattaaaaa tagattagga ggaattttga tgtttagaca ccagaaagaa
      121 ttgcagtttg aagcaaaacc agatcgtccg aatccattaa tcgcgaaata tctgcaggag
      181 ctgatcggcg gccaatatgg cgagatgagt gtagctatgc agtacctgtt ccaaggctgg
      241 agctgccgag gagaagaaaa atataaagat atgctgatgg acattgcaac agaagaaatc
      301 ggccacgtgg aaatgttggc gacaatggtt gctcgtttat tagagggtgc gcctgttgaa
      361 gatcaagaag cagcgatgca aaaagatcca acaatcggtg ccattatctc tggaatgaat
      421 ccacagcacg cgatcgtaga tggcttagga cctcgtcctg ctgacagcgt gggcaatcca
      481 tggagcggtg cctatatcat tgcgagtggc aacttgttag cagactttag agctaattta
      541 aatgccgaat cacaaggaag acttcaagta gcacgtattt acgaaatgat cgatgatcgt
      601 ggcgtaaaag atatgctgtc tgttctcctt gcaagagata gctatcatca aaatctatgg
      661 gaatgtgcag tgaaagaatt agaagaaaaa gaaggttcaa tcttagtccc aagtactttc
      721 ccgcgtgaca aagaacgaaa agacattgct tatgacttct ataacttctc agagggagac
      781 caaagtagtg aaggttcttg ggctagtggc aaagcactgg atggcgaagg cgaatatcaa
      841 tggttgaaag aacctaagat cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa
      901 atgtatggga cacctccatt caagaaataa atatctaccc gtagaaacac ccgttttttg
      961 ttccacacgc tttgtgagca aaactgacta aagtcattaa tcataaccac ccttcaaaag
     1021 ggtggttttc tctagagcta taagctcaaa tcaccggcca gcgcctaaag acgctggctt
     1081 tcatatttta tgttcaagcc atattgcctt tgccagtttt gcccacctct taaaaggttt
     1141 atgtactact ccttagccct taaaaggatc gtcttactct cgtacactca gcttatctaa
     1201 cgcactatca tgtttttctt gttcttgaat gtactttttg atggtcgctt cattttgtcc
     1261 tacttttgtc acatagtatc cttccaccca aaagtgtcga tacccaaatt tatattttag
     1321 atttgcacaa tctcttgatg atctcctgaa tattggctcg atattgatta taaattattt
     1381 ttcttctata ctttggtgtg aatacaatgt ggtatttaca catctacttt gtacgggcta
     1441 aactatttcc ctttattgct ccacccgcat agcaagtggt tttgtatttc aatcgctcgg
     1501 cgcgctcaat taggtctaaa gacaataaat ctaaaaaccc gctgcttcac tgacagaaac
     1561 aacgggtttt cagaaaaaaa tgaaatgaaa aagatctctt acggcaatct attttatttt
     1621 tttgattttt tataaatgga aaatgcttca agaagtccaa gaaataaagc cgtataaaaa
     1681 caagaaccta tgaaatcttt atttattaga tattcaacag aaatcccgat aacactagcc
     1741 aacaatatat ttatcagaag tgtaaaataa ggatgctttt tatagaatag aactagtgtt
     1801 gttttcatag actctccttt cttcatattt ccaattaatc acaaattcac ggcattacta
     1861 gaaaaattaa aggggcgcag aagtggaaaa atactcttcg actttcccta caaaccaggc
     1921 catcttgtgt ttaataacta taccaaatct aaggtacctc taaatctaaa ttccatttgt
     1981 ggcggtgacc tccaattttt aactcttcta tctcccatcc agtatacata accttgatac
     2041 ttcgaccact tatatatcga acacacaaat acctatccat tgcaggagta ctacctaact
     2101 tagttgctac ttgcatctaa ataacagctg aaaagatagg acttactctt agtaaagtat
     2161 ctgaactatt aatttttaaa ctatcatttt tatcctcaac tacatgcaca tctgttgcaa
     2221 cttcactttc tgtctcatcc agataaatga ttaaggaatc gggaattgca atatcttcag
     2281 ctgacgctgg aatagttgag ctaaattagt catcatatct ttattataaa ataaccaaca
     2341 gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa tgttgaaaaa
     2401 ttcagcaact cattactgta tgattttagc agcccaacca acatataccc tataaaagct
     2461 aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt ccttaaataa
     2521 cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc aaaataaaaa
     2581 aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc tctactgaca
     2641 taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat caattagaga
     2701 ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga ttctactttg
     2761 gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg tcgttagaac
     2821 aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg tatggatgtt
     2881 ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg cctttttgtg
     2941 ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa attttacgtt
     3001 tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt agatttttta
     3061 ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc gcgacttttc
     3121 gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt
     3181 gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt aagatacgga
     3241 aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct ttttttaatt
     3301 tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta aaacatatac
     3361 tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat ttttttgagt
     3421 tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc ttttatatct
     3481 agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt aattggacat
     3541 atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag tcctggtcgt
     3601 cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat atcacctttt
     3661 ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca ggagttaaat
     3721 cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat ggatcttcaa
     3781 tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat tcttggtttt
     3841 ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt ccttgtaatc
     3901 tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat ataacactaa
     3961 gtaacgcaaa attccccttt gtaaactatc tagaaaattg ttttgtttaa agtatatctt
     4021 attggatata aatagaaata aattgtagcc agttcatttt ttctttaact aaaaaaataa
     4081 tccaagaaac attgaaccta tggatccaaa agctacccaa atactatagc taaccccccg
     4141 ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca acaggccatc
     4201 ctatcatgcc cgattataaa caaaaagctg gctattcctt ttgctctgac aaatttcaat
     4261 tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc
     4321 cgcttcaaat agaaacattg gttcaacgaa tttcaactag acaagacttt gctattgtag
     4381 aacgtgagat cgtagctatt tttgtagaag aagggatttt acatgatcca actcatatca
     4441 atgtggataa atggcaacct cttatctata aattcagaga atatacaact gccaaccagt
     4501 cgttaacttt gaattttaga ttccaagaat tcaaagagtc ataatgataa acatcgttag
     4561 gtataaataa aaaagactcg cagcacgagt aaaaaacaaa tagaagctat acattagaaa
     4621 ggtcgactgt gaccctatag atgcagtata tgcagcttat gagcatttct tctgttctct
     4681 ttgtttttgg gatctgtgct gaagtctttt tcgttttttg ccgctctttc tttgaaaaat
     4741 cgattatgtt ataccttctc cattactaat tcaggcatct gctgagtaat gatttttcgc
     4801 gcaaaagaaa tatctgttac ccaaggttcg atttgttctt cagtcaagac aagcggcatt
     4861 cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag tcattatgat tgattctgcc
     4921 attttttcat cctgatggct acgataaaaa ccgccaatat acactatttt ttccttattt
     4981 gaaaacataa atttgtcttt ttcttcattc cactcattgg gaaaacacaa cgattttgca
     5041 aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca ttaatcatca
     5101 attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg aggatgtttt
     5161 cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca gctgctaatt
     5221 ctgctgcctg cttcggttgt ttcgtctggg cttctcgata ataattctga aggagctctg
     5281 actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt tttttctatc
     5341 atacactgca acaagaagtt cgcaccaaca aaacgctttc aatttttgta gaactaaaaa
     5401 agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact cctcggtcac
     5461 aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct tgtgactcgg
     5521 agtgaaacgg caggcttacg acctctgggt aaacggtgtt caaaagccag tttctcggta
     5581 ttaagccaaa tttatgcaaa aatgtagaaa gctattttca caaaattttt cttaatactc
     5641 gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc
     5701 ggtaataagc ccaaccaaac gaaaaatatg agcagctatt tttatttggc tgttcttatt
     5761 acttgatgca gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctaacc
     5821 ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta
     5881 atactcaggt caaagcgctg atttcacaac ctctaataca cggtgttcca gaagtagctt
     5941 cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc
     6001 ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa ccttttgcga
     6061 agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata gatcctgtgt
     6121 gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg cttgacgatc
     6181 tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt tcgtatactt
     6241 gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca ctgtactcaa
     6301 ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct catgatcagc
     6361 gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag catttaagat
     6421 gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg ctagacaaac
     6481 ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag aagctgctag
     6541 aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt tggtcgcttc
     6601 caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat agtcctatat
     6661 tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt gccaaattat
     6721 ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt gattcaagaa
     6781 ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc ctctgttgtc
     6841 atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc ttcttggcca
     6901 ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac caattcacgc
     6961 atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt gtcgattcca
     7021 ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt atgaggagag
     7081 ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc ggctcctgaa
     7141 ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc aagcatcata
     7201 tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata atgaggagaa
     7261 ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat attttcgatc
     7321 gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc tggtttcaag
     7381 aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa catggtggat
     7441 tctccattcg ttatttattt taatacatca tttagagctt cactcattgt cggatgagaa
     7501 tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag cgaaatcaag
     7561 ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa gtcagtttca
     7621 gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc tttagggaca
     7681 ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc ttgtttttct
     7741 gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac agctttccgg
     7801 ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg ataatcatct
     7861 agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca aatgttgtct
     7921 gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag tttaacatct
     7981 gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc caaaactttt
     8041 gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc tgttgattct
     8101 tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc atttgtcatg
     8161 tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc agaatgggca
     8221 tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc caagccaata
     8281 tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat cgcttgcgta
     8341 gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat tggttcagaa
     8401 ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt attcgtcact
     8461 tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc agttgcctca
     8521 tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc tttccgagca
     8581 acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt agaaggaata
     8641 cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac gactaaaacc
     8701 gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc aaaaccgatc
     8761 acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct accgattggt
     8821 acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta tttgtcaaaa
     8881 aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc ctttattctt
     8941 ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt aagggcagaa
     9001 ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat cgatatgcac
     9061 ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag agacacgtgc
     9121 ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta ccgccccatt
     9181 gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt ctgcatggat
     9241 tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt ctccattgcg
     9301 cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc tcctttcgat
     9361 tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct ttctacaaaa
     9421 attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa aacaaaaaaa
     9481 tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc ttttctacat
     9541 aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc ccataaacca
     9601 atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc tggtctccct
     9661 accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag cctttcaaaa
     9721 agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa taattttgct
     9781 aattttacta acgccaactt gttctttccg aattcatcat tttctatctg taatactagt
     9841 aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg ctcttctaga
     9901 tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc ttcttccaac
     9961 agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc gctgaacaat
    10021 tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg ttcatctaat
    10081 gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat gacatatgca
    10141 ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc gatggctagc
    10201 tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt tcgatagccg
    10261 gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag atgagaagaa
    10321 gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat tgtacttaat
    10381 actgcgatca aattatttcg attgatcact gttccagaag cactgacaac tgcagaacga
    10441 tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac agcctgcttg
    10501 atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa tcggccatct
    10561 ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac tttttcagca
    10621 aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt tagtggtttg
    10681 ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc ataaggtgtc
    10741 aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg ctgctgtgat
    10801 aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa ttttattcca
    10861 ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca cgagaactgt
    10921 tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc gatgacatgt
    10981 atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc gcttatccta
    11041 ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt cttctttttc
    11101 tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt caagtttact
    11161 tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg cagcgctttc
    11221 atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc ttttctgcaa
    11281 atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca ccggccatcg
    11341 ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca tcggcaccat
    11401 ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa acctcgcgtc
    11461 taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta aataatgtat
    11521 agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga atgtctcgag
    11581 aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc agctcttcct
    11641 gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc tgtttctttt
    11701 tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt tcttcatatt
    11761 ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt ttctatttta
    11821 acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct gatgtaaatc
    11881 tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc ttctaatttc
    11941 tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc acaaagacct
    12001 tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt gctagaacgc
    12061 tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat catcaattga
    12121 taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg cagtccccat
    12181 tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca atttttttta
    12241 ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg tttaaagtct
    12301 tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg aacctctagt
    12361 atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat tttttccatt
    12421 tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt tgagtgactt
    12481 ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt aaataaatcg
    12541 tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta taatagaaat
    12601 cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca aaaacagctg
    12661 cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt attgatcgag
    12721 acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac gactgcacaa
    12781 gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc gctgtctact
    12841 tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct gcttttcttg
    12901 gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt tggtcgattg
    12961 ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat cttgacattt
    13021 gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac ggccattgct
    13081 ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg gaatcatatc
    13141 ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt tgctattccc
    13201 gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg aatttgtctt
    13261 ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag ctggattgct
    13321 ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa agtggagcat
    13381 ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt gattggcttc
    13441 ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt ccttcagatc
    13501 gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc agcggttggt
    13561 gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa gaagccaatt
    13621 tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact cataggacac
    13681 gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat cggattatct
    13741 tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca aggagacggg
    13801 aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc attagtagcg
    13861 gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc tactacatta
    13921 ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt tgctcgattc
    13981 gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg tgttgcatct
    14041 gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg gaaaaaattg
    14101 ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat acatcaaaat
    14161 tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc gctgggatta
    14221 tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct aaagacggcg
    14281 ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt tacgcacaat
    14341 cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt cttgccgaga
    14401 aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct gaacagacat
    14461 ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt ggtggtcgtc
    14521 tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa caaatggagc
    14581 agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc aagggaccgt
    14641 ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt aagcctttat
    14701 tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc ttagcatttc
    14761 agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga gaagctgatt
    14821 tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac gatcaaggct
    14881 gggatttggc tcctactagc gaagcttata agggattgac taagagtaca gaacagttct
    14941 tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt acccgtgctt
    15001 atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc ttactggctg
    15061 ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat ctattagcca
    15121 acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc tccacctata
    15181 ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga gcgatattgc
    15241 gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc aaattctcca
    15301 aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca atgaatcgta
    15361 aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca aaagaaacag
    15421 gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac ttgaattctg
    15481 ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg atttttttat
    15541 catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa aacaactctc
    15601 gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca aatggttcaa
    15661 tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat cgttttgttg
    15721 ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata attggcggaa
    15781 gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga tgatcgactc
    15841 ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt atatctcctg
    15901 ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga caagtaagga
    15961 aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag taagaaggtt
    16021 ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca tgcaagatcg
    16081 aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca gaaccggaca
    16141 acccaaatac agccatcgta taggtctggt cattactagc atattgtttc atcccaccgt
    16201 ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa gtcgcttttt
    16261 ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga gcatcaaaca
    16321 aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag atataaagat
    16381 ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa ttcgctggct
    16441 gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc agcaaatgac
    16501 tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa aactggcgct
    16561 tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt tccttgtcta
    16621 agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca tcattgaata
    16681 ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt gttaaatctg
    16741 tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta tcaaaaactt
    16801 catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg gataacagtg
    16861 gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg gctcctcctc
    16921 attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt atttgtactg
    16981 ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa cttgaataaa
    17041 caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt ttgctaacca
    17101 gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt ctgctacacg
    17161 ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt gataacggcc
    17221 gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg cgatccattc
    17281 ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag ctacttcaac
    17341 tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg tagatgctgt
    17401 attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg ggattgttaa
    17461 ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag caattgcatc
    17521 gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat cacctgattt
    17581 tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg cagtagcacc
    17641 agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa actccttttt
    17701 ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata tttcataggt
    17761 aaaagaaa
//
LOCUS       DAYYAG010000054        17347 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000054 DAYYAG010000000
VERSION     DAYYAG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.054"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..588)
                     /locus_tag="ACHARQ_001561"
     CDS             complement(43..588)
                     /locus_tag="ACHARQ_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8409975.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(600..1292)
                     /locus_tag="ACHARQ_001562"
     CDS             complement(600..1292)
                     /locus_tag="ACHARQ_001562"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8409976.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1311..1595)
                     /gene="macP"
                     /locus_tag="ACHARQ_001563"
     CDS             complement(1311..1595)
                     /gene="macP"
                     /locus_tag="ACHARQ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8409977.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1608..2171)
                     /locus_tag="ACHARQ_001564"
     CDS             complement(1608..2171)
                     /locus_tag="ACHARQ_001564"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8409978.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2399..3061
                     /locus_tag="ACHARQ_001565"
     CDS             2399..3061
                     /locus_tag="ACHARQ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8409979.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3185..4381
                     /locus_tag="ACHARQ_001566"
     CDS             3185..4381
                     /locus_tag="ACHARQ_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8409980.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4482..5858)
                     /locus_tag="ACHARQ_001567"
     CDS             complement(4482..5858)
                     /locus_tag="ACHARQ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8409981.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5895..6762)
                     /locus_tag="ACHARQ_001568"
                     /pseudo
     CDS             complement(5895..6762)
                     /locus_tag="ACHARQ_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6851..7876)
                     /locus_tag="ACHARQ_001569"
     CDS             complement(6851..7876)
                     /locus_tag="ACHARQ_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8409982.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7894..8568)
                     /locus_tag="ACHARQ_001570"
     CDS             complement(7894..8568)
                     /locus_tag="ACHARQ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8409983.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8752..9645)
                     /locus_tag="ACHARQ_001571"
     CDS             complement(8752..9645)
                     /locus_tag="ACHARQ_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8409984.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9719..11416)
                     /locus_tag="ACHARQ_001572"
     CDS             complement(9719..11416)
                     /locus_tag="ACHARQ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8409985.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11409..12722)
                     /locus_tag="ACHARQ_001573"
     CDS             complement(11409..12722)
                     /locus_tag="ACHARQ_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8409986.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12722..16663)
                     /locus_tag="ACHARQ_001574"
     CDS             complement(12722..16663)
                     /locus_tag="ACHARQ_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8409987.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16772..17230)
                     /locus_tag="ACHARQ_001575"
     CDS             complement(16772..17230)
                     /locus_tag="ACHARQ_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8409988.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4861 a         3896 c         3014 g         5576 t
ORIGIN      
        1 tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa tttctgcgcc
       61 tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga aacttgccac
      121 ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg ctgtatgcaa
      181 cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc gttctcgcaa
      241 acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta tccaataaac
      301 attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca attcccgtcc
      361 acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa aggaatcttt
      421 tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa acttctttgt
      481 atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca gctttccatc
      541 ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct tagtcctcct
      601 tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg
      661 ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg
      721 atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc ttccatttcg
      781 caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg cgagtcgaca
      841 aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc ttttgctgct
      901 ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat tcctggtaat
      961 tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac tttgtctgat
     1021 ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc tgtattgatc
     1081 accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc taagactttt
     1141 cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga aataaataaa
     1201 gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat ttttacttct
     1261 tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt ctatataaat
     1321 gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga tttcatcaaa
     1381 aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt gattggttta
     1441 ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg ctgataagct
     1501 gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga
     1561 agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta ctttcctttg
     1621 gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat caactgatct
     1681 ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac ttcgtcttca
     1741 tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat atgaaggact
     1801 tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga cttcgcacga
     1861 tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg tccttctcct
     1921 ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt tcgaaattgt
     1981 ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc gccaggatga
     2041 aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc tacagcaaca
     2101 tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc aaattgctca
     2161 ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt ttcatttttc
     2221 aatttttctc aaaaactcct tattagttta ccatagataa accttagatt ttctctaaat
     2281 atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta aaataagtat
     2341 gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag ggtttattat
     2401 gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct ttgtccgaaa
     2461 caatagtttt tcttttgttc taggactttt attgataggt gtagttttag ttttgtgggg
     2521 actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac tgactgacga
     2581 acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat tttttcgaca
     2641 aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc agcagacagc
     2701 caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag ctttattcaa
     2761 agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt atacgcatct
     2821 gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg agatcaaaaa
     2881 caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt
     2941 aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg acgtagagat
     3001 atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac aaaaggatta
     3061 actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct gtttcgagta
     3121 ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca aggaggaatt
     3181 ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg atacacttga
     3241 agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt cacaacaagc
     3301 ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca aactaccggc
     3361 ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg atgcacagtc
     3421 tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac aatcgatgtt
     3481 gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg aactgatgta
     3541 tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca tcttccagcg
     3601 tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg
     3661 tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc tgctaaaaga
     3721 taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg cactgaatat
     3781 ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca ttccagaagc
     3841 gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca atgattacac
     3901 gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta gacagatcac
     3961 gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa
     4021 gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt
     4081 gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg aaacaacaaa
     4141 cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga
     4201 aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg atttgatcga
     4261 aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga
     4321 agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt taacccaata
     4381 gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac aactgttgat
     4441 gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc attcatcttg
     4501 ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac gataccgaac
     4561 acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa aatggagacg
     4621 attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt gccaaacatt
     4681 tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc catgatattc
     4741 gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc tgccacacca
     4801 gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt cataaactta
     4861 ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata catcatcgag
     4921 atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc cactgttcca
     4981 tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc agtatacgga
     5041 acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa
     5101 ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc tgccattgca
     5161 acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag cacaccggaa
     5221 aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg
     5281 ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa acgtaatgct
     5341 ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac cgcaatcaag
     5401 tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg aagttcggcc
     5461 cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac agaggaaacc
     5521 gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat
     5581 acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc aaattctgcg
     5641 aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag tgacagtgct
     5701 ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt accgatgatc
     5761 gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat ctcactcgta
     5821 tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc ccattttagg
     5881 ttggaaaaca gcgattaaac tgttttcata taccctaaaa gggataccac ctaaaatgac
     5941 taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt cgccaaggat
     6001 gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga agccaacagc
     6061 taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc ataataatcc
     6121 acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag caaagaagct
     6181 tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat ccaaattcgc
     6241 ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca atgctctaaa
     6301 acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt catcttgtcg
     6361 ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt atacataatc
     6421 tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca aaattaattg
     6481 aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc catggaactg
     6541 tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga aaatccaagg
     6601 ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg caccgatcgt
     6661 cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta aaccaatact
     6721 accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc tccttttatt
     6781 tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt ttttcgtttc
     6841 taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat aaagtcattg
     6901 ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt tcggccagat
     6961 tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga ccatttctcc
     7021 cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg atccctgaat
     7081 ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg atgtttccgt
     7141 gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa gcgatccatt
     7201 tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata tttttttgga
     7261 taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt ccatattctt
     7321 ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact tgttctacgt
     7381 attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg tcaatatcga
     7441 actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt tcatggaccc
     7501 atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc attacttctt
     7561 ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct tctgtcctcc
     7621 caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat tttccccacc
     7681 atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg ccctcaatca
     7741 aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa
     7801 agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga tctttcaaat
     7861 atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc cctgtcctac
     7921 ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta accgattgat
     7981 gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa
     8041 tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata gacgatattc
     8101 atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt caacattcaa
     8161 cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt aagaacggcg
     8221 aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg tcacaaaatc
     8281 atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc tggagataaa
     8341 aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc catccaaaac
     8401 gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt ctgagaacac
     8461 ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt cctccatgat
     8521 caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa aaaatccctc
     8581 catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt tggcttacct
     8641 ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt
     8701 gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct attatctgtt
     8761 atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac cgaaaagcgt
     8821 tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt atttttttgt
     8881 ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg tcactagtaa
     8941 tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat cctgcttttc
     9001 tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa cgacttcata
     9061 agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca tttcttcaag
     9121 atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg aatgtgtact
     9181 ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat ctaacaaaag
     9241 atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat agatggctcc
     9301 gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa ccgcttgctc
     9361 aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa gcgtctgatt
     9421 tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc gttcaagctg
     9481 cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat ctgctaaaaa
     9541 tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata attgtaataa
     9601 aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct tcaccttctt
     9661 atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt tttgttgctt
     9721 atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc atcaaactta
     9781 ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt gacccgcgtt
     9841 tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga atttcttcat
     9901 tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt ggaagtacat
     9961 aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac ccttgaacct
    10021 caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa
    10081 ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa aatattgcgt
    10141 attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat tcactgcttg
    10201 atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc ggctctactc
    10261 cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga
    10321 aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt aaatctcctc
    10381 cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt tcttctgtca
    10441 aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt ccttttgtta
    10501 aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga ccaactgtag
    10561 gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg acagaatcga
    10621 tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt tctagatcaa
    10681 tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt ctaaggtcgc
    10741 tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga tacaaatgca
    10801 catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc accatatcag
    10861 gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata tgagctggcg
    10921 aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct tccgtcactt
    10981 ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca atacttgtca
    11041 gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca atcgcttcgg
    11101 cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc aaatcgtaat
    11161 atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct tcaccaaaat
    11221 ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa ccttttttgt
    11281 gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg actagttttc
    11341 cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt cccgtgacaa
    11401 tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac agcacctaac
    11461 aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg cgggaatacg
    11521 gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg tttgtccgtt
    11581 gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc agagactgga
    11641 actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata ttgtggttgg
    11701 ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact tgcttcatcc
    11761 atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga aggcaagaaa
    11821 cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac atttttgtca
    11881 ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt agcaggagcc
    11941 atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac agcaccttct
    12001 aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa gtatggatct
    12061 cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc ggttgattct
    12121 gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc gatatcaaca
    12181 tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc atcccgatct
    12241 tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc aaccgtatct
    12301 ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat tagctgacgg
    12361 atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt catgttttca
    12421 gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt cgctccattt
    12481 agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga gatagccaag
    12541 ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat catccgttta
    12601 tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc atgtggaaga
    12661 aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa cagctttttc
    12721 attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct gctcctgcaa
    12781 gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct cctgtagcag
    12841 gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga tttgtctcca
    12901 aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg tttggataat
    12961 acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga tcattttcta
    13021 tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt tttctatagc
    13081 tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct gaagcattga
    13141 atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca tactctcctg
    13201 cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta ttcatactgt
    13261 caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt acttgtagta
    13321 caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg atgaccgtat
    13381 cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga tcagatagcc
    13441 gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac agatgcagag
    13501 gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa tagaagtatc
    13561 cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg tatgctcgat
    13621 acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca tctaatgtat
    13681 ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta ttgtaagcag
    13741 tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat tgccaatttt
    13801 cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt cgcaagatcc
    13861 atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca atgattttgt
    13921 attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct ggctctccga
    13981 ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca tctactgaat
    14041 agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc cctttcgcta
    14101 ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac tctgcgccat
    14161 tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt ggcttgaatt
    14221 catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct atgttaggct
    14281 cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga tctttacctt
    14341 ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag tgacctaaca
    14401 tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca tctactaact
    14461 gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact gctccagttt
    14521 catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg gtagttttac
    14581 ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat gcaaattcga
    14641 ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta cggcctttat
    14701 gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata tcattctcat
    14761 aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt ccgtcttgag
    14821 ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca cctaaagaaa
    14881 tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga gtcgccacta
    14941 cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct tggtacttca
    15001 tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg ccagctccaa
    15061 atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc gttctcttga
    15121 aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc gcattgtaat
    15181 atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca tttcgtccaa
    15241 tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca atggagaaga
    15301 tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca actgtgttga
    15361 tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta gaagaaccat
    15421 caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa cgatagtgta
    15481 catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga tgagtaaacc
    15541 ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg tcattatctt
    15601 cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg ctcgtacgat
    15661 aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca acgattagga
    15721 ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca tagccatttt
    15781 taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct gtattctcac
    15841 ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg atttgctcat
    15901 ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga atatccagac
    15961 tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac gcagcatttt
    16021 tagtttgact attggtccca gcggttaatt cactcatact cgctgactta tctagaacaa
    16081 aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct agatagacat
    16141 tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt ggcagaacag
    16201 gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt tcaaaattcc
    16261 gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct tcgcctgcag
    16321 ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg tttgtataat
    16381 cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct tgatagcgtt
    16441 cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta aacttcgggt
    16501 agcgatgctg ttcgttttca acttctccgc taggatagat atattcatag gggtcttgcg
    16561 tagctgttgg cccaagacgc tccaaaatac cgttgaataa ctggcgttga atgcgctgga
    16621 gtgcttgacc gctgggtacg attggttcgt ccagcttttc catctcattt tcttcgctac
    16681 gttgaatttc agcggaatct tctgtatcag tttcattttc ttgtaattct tggttatcta
    16741 ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg gggatctttt
    16801 ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt ccgacatacc
    16861 attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca taggaggata
    16921 gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca tcgattgcta
    16981 aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc cattcaaggg
    17041 tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct aagaatacct
    17101 catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata tgatagactg
    17161 actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc ttcaatttta
    17221 accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca acaatcaaca
    17281 aaacaatgcc catctaatta taatttaatt taattttaat tttaaatcaa atgatatcct
    17341 ttttagt
//
LOCUS       DAYYAG010000055        17259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000055 DAYYAG010000000
VERSION     DAYYAG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.055"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..558
                     /locus_tag="ACHARQ_001576"
     CDS             121..558
                     /locus_tag="ACHARQ_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8409989.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            653..1537
                     /locus_tag="ACHARQ_001577"
     CDS             653..1537
                     /locus_tag="ACHARQ_001577"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8409990.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1761..4928
                     /locus_tag="ACHARQ_001578"
     CDS             1761..4928
                     /locus_tag="ACHARQ_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8409991.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4996..5493
                     /locus_tag="ACHARQ_001579"
     CDS             4996..5493
                     /locus_tag="ACHARQ_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8409992.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5512..5976
                     /locus_tag="ACHARQ_001580"
                     /pseudo
     CDS             5512..5976
                     /locus_tag="ACHARQ_001580"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5973..6731
                     /locus_tag="ACHARQ_001581"
     CDS             5973..6731
                     /locus_tag="ACHARQ_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8409993.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6817..7557)
                     /locus_tag="ACHARQ_001582"
     CDS             complement(6817..7557)
                     /locus_tag="ACHARQ_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8409994.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7618..8871
                     /locus_tag="ACHARQ_001583"
     CDS             7618..8871
                     /locus_tag="ACHARQ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8409995.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9193..9420
                     /locus_tag="ACHARQ_001584"
     CDS             9193..9420
                     /locus_tag="ACHARQ_001584"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8409996.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9543..9869
                     /locus_tag="ACHARQ_001585"
     CDS             9543..9869
                     /locus_tag="ACHARQ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8409997.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9937..10650
                     /locus_tag="ACHARQ_001586"
     CDS             9937..10650
                     /locus_tag="ACHARQ_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8409998.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10960..11810
                     /gene="licT"
                     /locus_tag="ACHARQ_001587"
                     /pseudo
     CDS             10960..11810
                     /gene="licT"
                     /locus_tag="ACHARQ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            11931..13796
                     /locus_tag="ACHARQ_001588"
     CDS             11931..13796
                     /locus_tag="ACHARQ_001588"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8409999.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13810..15253
                     /locus_tag="ACHARQ_001589"
                     /pseudo
     CDS             13810..15253
                     /locus_tag="ACHARQ_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15361..16062
                     /locus_tag="ACHARQ_001590"
     CDS             15361..16062
                     /locus_tag="ACHARQ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8410000.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16188..16700
                     /locus_tag="ACHARQ_001591"
                     /pseudo
     CDS             16188..16700
                     /locus_tag="ACHARQ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5758 a         2901 c         3711 g         4889 t
ORIGIN      
        1 tgatttagtt ctttttattt taaaaatttt ttttacttag aatccagcga ccctagatat
       61 ttttctgtat aatcttaaat aaagaaaaca atatcaaaaa atgcacgtag aagtagataa
      121 atggagtggt tttgcaaatt tcttttattt ctagaagtta ttcacgtata cggatttgtt
      181 tttctcacaa ataaaaaggc aggaagtaca atggatcatt tgtatataaa atatattcat
      241 caattttccg ttttggtgtt aggcacacta attatagcgg ggattgtcgc atttattatt
      301 caaggccgtg acatattaac agtacatcaa gagtttttca gttatgcatt tggtattcct
      361 ctgatcgcta taaggtctga taacaaaaag tggaagaaac gctattacat tctcttgcta
      421 tttttgtgtt tttatctgcc acctaagatt tttactttat taggtataca atgcctatca
      481 ttttctttta tttcctatat cattgtttta attttcctca gtttcatagt atggatgttt
      541 tatcttgtca aaaaataaat gaatgtttca tgaacagtac ttcatcacag cctaattttt
      601 ttctttatgc tatcgttata ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa
      661 agttttatta gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt
      721 agcgatagca aaattggaat cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc
      781 aggggatgca aaaaacttta ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt
      841 catgggcgga gatggaacag tcaacgaagg aatcagtgga atcgctgaac aagaacatcg
      901 gccaaatttc ggatttttcc cattgggtac ggtgaatgac ttggctcgag cattagggat
      961 accgctagaa cctgaggaag cgatcaatca tttttcgatt gaatcagtca agccattgga
     1021 tattggaaaa ataaatgacg actattttat gaatgtcgtc gcaattggct ctatccctga
     1081 atcaatcaat gatgtagatg cggagaagaa gacgaaattt ggaaaactag cttatttcat
     1141 gtcaggaatc aaacagttag cttccacaca atcctactcg tttcacgtag aagttgatgg
     1201 aaagaaggaa gaaattgaga gcagtacctt gctgatagga ctaactaact cagtcggcgg
     1261 ttttgaaaca ttacttccaa atgcaaaagt ggatgatggt aagctccatt tggtttattt
     1321 aaaagattct tctttgatcg atacgcttaa aacattgcca gacctattaa aaggagtaga
     1381 tgaatctacg aaaaatctag cctaccttac ttgtgagcaa ttagttgtgt cattagctga
     1441 tgaggaagaa ttgacgacga atgttgatgg agatgaaggg gataaattac ctgtgacaat
     1501 caaagtattg ccctcacatt tacaggttta ttgttaaaat ggaggttgtg accgaggagt
     1561 tggcgggaga acaccgcttg ttcgggataa gggactgtga caagactttt gtcacagcct
     1621 tttttttgta caaaaaagaa atttacacct aatagaaaaa tttttccata aaaatacata
     1681 ttttaaatat gaaagtacaa ttggaaatag attaaaacga taaaataaaa ttgtcaaaac
     1741 gtcagagagg atggtggaga atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt
     1801 ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa acaaacgaat
     1861 caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa tcgattactt
     1921 ctgaaactac agaatctcaa agtgaggctt ctactaataa aaggcaagaa gaaaataaga
     1981 caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa
     2041 gagctgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg gcgcagcttc
     2101 tcgcagacga atcagtaact aagatccgac tgattcagcc tttgacttta gacagggaat
     2161 tagagatcaa acgagatatc gttattgatt ttggtggatt cgctcacaat ttcggcacac
     2221 accatattta tatcaatgag atacctcagt cgatcgagtt ccagaatttt aaaggaacag
     2281 ctgcacatcc aggtggattg acaccaaata tcgatggaaa tgccattatc attgcttata
     2341 tgaccgattt ttggggaagc agatatcatt ttacgggaga aataaaattt accgggacac
     2401 ttgatttgta tgatgggtcc aaattaggac tgatttacgc tcctcgtgca actgtcacat
     2461 tagatggtgt ttccggtgtg ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc
     2521 cgggtaaagc ctatttttgt agaagctatc aattgaatgt gatcaatggt agtcaacttt
     2581 atggaccgta tctagggaaa ttctacggtt ttctagattc aggagatgag actggtagtg
     2641 gaaaaacagc gccaggaatc catatcttaa gcggttcaaa ggtctctctt gattatgatc
     2701 gggcagatgg cgttagcgat gaaggagagg cagtcgatac gctacctggt aatgtcatgt
     2761 ttgaagtcag tggttcggga agcgaatttt cggtaaatac aaagatcaat attaccaatg
     2821 acaacaatcg cggaatcatc caaatgagag gttcaggatc gaaagtcact gtatcgaatg
     2881 gtgggaaaat caccatcaat acagcaacta cgggcggatt tcgcttgcaa ggagattcta
     2941 gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga gatgacgatc
     3001 aacccgggaa caatgggatg cgttttgtag gaaaaggcct ttctttgatc gtagaaggag
     3061 ccggatcagt tatcgacatc gataagcaga gtgggcgttc cagcgcagtt cgttttgaga
     3121 atggtacgca aaagctgaca gtaaccaatg gaggctcatt gagggtaaga aatacaggtg
     3181 atggcaaatc ttacgtgaac agactcaatc aaggaaatca agcgatccaa ttttatgacg
     3241 cttctggttt tctagaaagt ccaggttcag ctgattttac agtcactgga gaaaaatcaa
     3301 atatcgatat acgcgcagat tccggagcaa ctgtagatac aactggagat attgatttaa
     3361 attttacggc aggtgaaaaa acttatttag tcatggtcgg gaaaacaggg gaagataaga
     3421 ccggcatcat cagtggggat gtgttgaatg tggacttaga atctcctacc tattttgatt
     3481 tccaaaacaa acggattgga agcgagacca atggcatagg cggctgggta ttccaaggaa
     3541 atggagattc ttctttaaag atcgcccatt cagaaatcgc tttatggcga aaaacaggcg
     3601 caacattcga taactttgaa aaagacccgg attatttttc tgatatgaca gacttatcga
     3661 tcaatgggac cgacttaggc aatttcgtat caagccctag tgcaggttta acaaatgaat
     3721 ttgctcagac aggtgccgga atgaaaaatt ataatcgaat cagtgccaat aatcagctcc
     3781 cgatgattga atccttaaga gtgccaacga atgcagataa aaaaatctat gggcatgtcg
     3841 ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga
     3901 ctttacgtat tacttacgca gatacgacga aatcatctgt agaaattact gctctcacgc
     3961 aaggacaaac agatgaaaat gaaggggcag gatataaccc atggggagaa ggcgaacaag
     4021 gcggcttgtt cgaagttcaa gtacccaatg atgagagatt aaaaacggag gatcaagtgc
     4081 aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt gacagaactg gactctgcca
     4141 aaacgacagt cgatgtgatt cctcctgaac ctgctaattt aagtaccaca ttgatcaatg
     4201 cagcttctcg cacgatcaca ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa
     4261 atcaagctgt tttgtcagaa acaactattg atgatacagg ggaatttgtt ctttctatcc
     4321 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
     4381 aagctactgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
     4441 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
     4501 tcgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgatttt ggtcagatca
     4561 aagcaactgg tttgacacaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
     4621 tttatgatca gcgagaaacg aaaaatccgt ggatcttaac gataaaatta tctcagcctt
     4681 tcacaaatga tgcaggattt tctatgcagg atgtcctata tttcaagaaa ggagaggagt
     4741 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
     4801 aagcagatta ttcagaatat ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag
     4861 aaaatcagat agctggtaat tactcagcag agataacatg gacattagca gatgcgccga
     4921 cagaataaag atcaaggagg atgattgatg aatcgaatat ggaataaaag tcatcgaaac
     4981 aactgctctt tacttatgat ccttctcctt ggattcatcc tttggcagac acctgtccat
     5041 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
     5101 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
     5161 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
     5221 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
     5281 gatatgaaaa acttagcaaa aacagtggga gaaataactg ttcctcctca aagtgcggtc
     5341 actgagacac tttcactgaa agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg
     5401 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     5461 cagttttctc aagtgaaagc aatcttgatt tgaaatagcg aaaaagagtc ttccccagat
     5521 ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     5581 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     5641 aaagaaacaa aagagcctta cctttcaata aaaaaagaaa aaatagatgt agcacccaat
     5701 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     5761 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     5821 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     5881 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     5941 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     6001 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     6061 ccactacaga aggaacgatt tctgtgacca aaccagatgc tgcaaatcct gaagacgttg
     6121 ttccagttcc tcctgttgat ccaaatgatc ctgatactcc ttttacacct gacgaaccaa
     6181 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     6241 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aagcggaacg gacagtactg
     6301 gagcacaaac acaagtacag aatttcgtac aagtaacgga taaaagcgga aattatgccg
     6361 gttggaaatt atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     6421 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     6481 gtggaatgcc atctaccgtt tctggaactg gcgctgaagg aatcgaaatc ccagcagatc
     6541 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     6601 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     6661 atgctgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     6721 aggaagcgta aaatcatatc aacgatttca gggagtggag ccgattcaag gcccactccc
     6781 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     6841 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     6901 gttgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     6961 acagtaagat tttaaaagta aaaagtaagc atacttttct aactctgtct tcaattgttt
     7021 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     7081 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     7141 ttgatcaata aagattttat cgacatttac gatctgctgt tctttccaat tgatctgttc
     7201 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     7261 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     7321 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     7381 caagtcgata tcgataaaga aaacatcgtg attatcaata gtgcccaact ggatcaactg
     7441 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     7501 tctgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     7561 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     7621 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     7681 ttcttttcga aacaactgct agtaaaaaac agctttggtt gtatagcttt gatggtggtg
     7741 acacttttac ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     7801 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     7861 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     7921 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     7981 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     8041 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     8101 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     8161 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     8221 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     8281 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     8341 gaaacgttgg aatttttaga aatattagaa gatcattctg cagctatttt tgaaaaaccg
     8401 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     8461 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     8521 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     8581 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     8641 ggatcacgtt ttagtatagc gaacaaggca aatccaacga atttgacgct atcggagatg
     8701 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     8761 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     8821 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     8881 aaaggataag tttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     8941 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     9001 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     9061 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     9121 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagca aatccctacg
     9181 atcacaaaaa aaatgctgac ccagcagtta agagagttag aacaagatga aagtatccat
     9241 cgaaaagcct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     9301 tcattaagag agattctagt agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa
     9361 aaagcctcaa gggaagaaga cacgctactg catacacatg gtggctacct aaattattga
     9421 ttttctatga catttatttt aaataaaaga aaacaggagt ttttttaaaa gcatcatcta
     9481 ttttttatgt aaaaaaatgg tatgattaat caagtaagaa gtgattggaa gaaggcaagc
     9541 aaatgaatag tatggaaaaa gtcaaccagt ttagagatga acgtaattgg cgacaatttc
     9601 ataacgaaaa agatttggct atctctattt ctttggaagc atctgaattg ttagaacttt
     9661 ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt agaacgaatc aaagaagaac
     9721 tggcagatgt attgatctat tcatacatga tggcagacaa ccttcacctg gatctagatg
     9781 agatcattga agaaaaattg atcaaaaata atttgaaata tcctgtagaa aaaagcgcag
     9841 gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa
     9901 aagaaaaaca gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa
     9961 agatggctag tcaatatcct cttgttcctc ttgttattag taggactagc tttgattttc
    10021 aatgaacaga tcaaggatta ttttgtcaga gagacaggag ataaatatgc tatagcgaat
    10081 gtaacaaaag aagatctgaa aaagaataac gataaagatg tcagttttga ctttgatgcg
    10141 gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac agatctacca
    10201 gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt taccggtttt caaaggcttg
    10261 gataatacgt cactacttta cggagcagga acattatctc cagatcaaga aatgggaaaa
    10321 ggaaattatg ccttagcaag ccatcgtgca acgaaccctg aattgctgtt tacaccgcta
    10381 gaaaatttag agatgggtgc caagatctat ttgacagatt tagaaaatgt gtatacatac
    10441 aaaacatttt ttaaagaaaa agttgctcct acagataccc aattactgaa tgaagtcgag
    10501 ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg acgccgtcac gcgactagtt
    10561 gtacaaggag aactggaaag tgttacatcg ataaaagatg cgacagatga tatgagatct
    10621 gctttcaatc tagagacaaa aacattttag gatactggga cataagtcgt tacagattaa
    10681 aaacagccaa aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg
    10741 caaagaggtg catctctgat ttggttgttt tctagataga cttgtgttcc agttttttta
    10801 ttttttatat gaaaaggctt gcataaataa aaattcgatg gtatgatgat tttgaataag
    10861 aaatggattg ttactgtacg gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa
    10921 ttctaggttt ttttattttt cttgagaggg cgaagcaaaa tgaaaattga taaaattttg
    10981 aataacaatg tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa
    11041 ggactagctt ttcaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
    11101 tttgtttaag agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg
    11161 cagaggagat cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg
    11221 aattttcgcc taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag
    11281 caaaagaaaa tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga
    11341 aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac
    11401 tttctgagga tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg
    11461 aagacatgca agtgacgatg accgctacca caatggtaag agatatactg agtatcatca
    11521 gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc
    11581 atctgcagtt ttttgcccag cgttatctca aaggggaagg atctgacgag caagatgaat
    11641 ttctctacgc attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca
    11701 atgagtatct tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa
    11761 cgattcatat tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac
    11821 tgtatggcag gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt
    11881 ttcaatctgg gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc
    11941 aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag
    12001 tacattgtgc tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa
    12061 agttaaaaga agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag
    12121 tgattggcag taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag
    12181 tttcggagga tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct
    12241 ctagtatatt tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct
    12301 tatcattggc aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt
    12361 ttgctgcagc agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta
    12421 aaaagttcaa agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag
    12481 ctatcacagc agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc
    12541 tttcaccatc tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa
    12601 gtaaattaga gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc
    12661 cgcttgtcgt tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga
    12721 cggttgccgg taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg
    12781 tagcaggtct aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg
    12841 gattcgtacc gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa
    12901 tgcttcttcc agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa
    12961 aagatacgaa acttcgagca ttagggattt cttctacagt gacttcgtta tttggaatca
    13021 ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta
    13081 tttctggagg tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca
    13141 gtttggtcag cttattgacg attccgactt tcattaatac ggtggatggt gtagagtcaa
    13201 atgtgacagt agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat
    13261 tgattttagg atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg
    13321 caggagaacc gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac
    13381 tgccattgag tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg
    13441 ctattgatcc agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt
    13501 ttccaactgg acatgctgta gggatcacaa cgactgacgg agcggagatt ttgattcata
    13561 tcgggatgga tacagtagaa ttaaatggga atggctttga gatcttagtc aaacaagggg
    13621 atctcgtcaa agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg
    13681 gctatagcgt aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg
    13741 aattagacca aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag
    13801 gaaatcagga tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc
    13861 aaatcaatta gaaggggctt atgatcgaga cggaaaagga ctatccgttg cagatgctat
    13921 gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga
    13981 tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat cattacgatc gtttcaaaga
    14041 agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac
    14101 acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga
    14161 tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta
    14221 cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga
    14281 cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg
    14341 gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt
    14401 aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt
    14461 tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg
    14521 catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac
    14581 aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata
    14641 ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca
    14701 aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta
    14761 ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt
    14821 gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc
    14881 agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat
    14941 tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga
    15001 ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg
    15061 tgtagatttg atgggctata cgccttgggg atgtatcgat cttgtcagtg catcaacagg
    15121 agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac
    15181 actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg
    15241 cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt
    15301 gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt
    15361 atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc
    15421 tcaggaaaat atccagtcgg ttcgatgttg ccgacagaaa gtgaattaga agaactgttt
    15481 caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc
    15541 gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc
    15601 aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg
    15661 ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag
    15721 caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt
    15781 cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg
    15841 tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca
    15901 gtatttttaa cagaagaaga acaaggaatt ctgaaaacag acgcacagat cggcatcaaa
    15961 cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac
    16021 catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg
    16081 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
    16141 tcgtttggtt aggcttatta ccgcagatac acggctgttt ctggattacc atttattctg
    16201 gataaggggg tgtggcacga ttttcagaag gaatctatga accaatcact gtattgattt
    16261 tagcttcgat ggtaacccca ttcggcttga cgatcgctta tttcttaggg aaaatcattc
    16321 gtaaaaatat actcaatcgt caagaaatcg atacattgaa gaccgctttt cctatgggga
    16381 tctgtcagat cacagaggga tgtttcccta tcgtattgaa cgacttggca cgcaatgtca
    16441 tagcaacggg agtcggtggg acagtaggtg gcggattaag catgttttgg ggcgcagata
    16501 gccgtattcc ggcatcggga atgtttgcag tcgctacgat gaccagacca tgggcattca
    16561 ttggtgcatt gttagctggt tcttttgtca caggaataac cattttatta ttgaaaaaac
    16621 cagtagatcc gaatgcagaa atcatccaag aagaaaaaga ggaagaagac atatcttggg
    16681 atgatttgac aattagttag gagaggcaga atggattaca agaaaaaagt acaatagatg
    16741 taccttgaga caggaatggt atgaaagaat cagagttaga aagaatcgaa tatacagatt
    16801 ttggtttagg agcgatcgaa actgtccaga gcatcggcac cctccttgaa atgacgatga
    16861 aggaaaaatg agaacctttc gttgtcgaaa gggtctggta taccttatgt cgaaggaaag
    16921 gaaacaaaaa aactttcctg tactattcca aaaaggaaag aaagttatta tacagtaaaa
    16981 aaagaaatta ttcttcatgc tagagatcag tacacgttcg agcccaatat caaacactag
    17041 ttccaggcag gcgcaacagg tgcggttatc actgaatttt cttccagcag tgacgattca
    17101 agtgatattt ttacaaatcc agctatttat cgttaacaaa aagattttta ggagaagctg
    17161 atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg gcttttttat ttagagaaac
    17221 atcatttatc agtgaaacat aacacgagaa caaaatgat
//
LOCUS       DAYYAG010000056        16844 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000056 DAYYAG010000000
VERSION     DAYYAG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.056"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..982)
                     /locus_tag="ACHARQ_001592"
     CDS             complement(2..982)
                     /locus_tag="ACHARQ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8410001.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1006..2490)
                     /gene="galT"
                     /locus_tag="ACHARQ_001593"
     CDS             complement(1006..2490)
                     /gene="galT"
                     /locus_tag="ACHARQ_001593"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8410002.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2502..3491)
                     /gene="galE"
                     /locus_tag="ACHARQ_001594"
     CDS             complement(2502..3491)
                     /gene="galE"
                     /locus_tag="ACHARQ_001594"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8410003.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3740..3907
                     /locus_tag="ACHARQ_001595"
     CDS             3740..3907
                     /locus_tag="ACHARQ_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8410004.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(3969..5780)
                     /locus_tag="ACHARQ_001596"
     CDS             complement(3969..5780)
                     /locus_tag="ACHARQ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8410005.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5777..6142)
                     /locus_tag="ACHARQ_001597"
     CDS             complement(5777..6142)
                     /locus_tag="ACHARQ_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8410006.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6305..6700)
                     /locus_tag="ACHARQ_001598"
     CDS             complement(6305..6700)
                     /locus_tag="ACHARQ_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8410007.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(6718..7680)
                     /locus_tag="ACHARQ_001599"
     CDS             complement(6718..7680)
                     /locus_tag="ACHARQ_001599"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8410008.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(7680..7892)
                     /locus_tag="ACHARQ_001600"
     CDS             complement(7680..7892)
                     /locus_tag="ACHARQ_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8410009.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(7913..8611)
                     /locus_tag="ACHARQ_001601"
     CDS             complement(7913..8611)
                     /locus_tag="ACHARQ_001601"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8410010.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(8631..9173)
                     /locus_tag="ACHARQ_001602"
     CDS             complement(8631..9173)
                     /locus_tag="ACHARQ_001602"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8410011.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(9305..10309)
                     /locus_tag="ACHARQ_001603"
     CDS             complement(9305..10309)
                     /locus_tag="ACHARQ_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8410012.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(10306..11295)
                     /locus_tag="ACHARQ_001604"
     CDS             complement(10306..11295)
                     /locus_tag="ACHARQ_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8410013.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(11292..12098)
                     /locus_tag="ACHARQ_001605"
     CDS             complement(11292..12098)
                     /locus_tag="ACHARQ_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410014.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12264..13220
                     /locus_tag="ACHARQ_001606"
     CDS             12264..13220
                     /locus_tag="ACHARQ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8410015.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(13297..13815)
                     /locus_tag="ACHARQ_001607"
     CDS             complement(13297..13815)
                     /locus_tag="ACHARQ_001607"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8410016.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(13903..14052)
                     /gene="rpmG"
                     /locus_tag="ACHARQ_001608"
     CDS             complement(13903..14052)
                     /gene="rpmG"
                     /locus_tag="ACHARQ_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8410017.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            14280..14726
                     /locus_tag="ACHARQ_001609"
     CDS             14280..14726
                     /locus_tag="ACHARQ_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8410018.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(14921..16816)
                     /gene="asnB"
                     /locus_tag="ACHARQ_001610"
     CDS             complement(14921..16816)
                     /gene="asnB"
                     /locus_tag="ACHARQ_001610"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8410019.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         5074 a         3588 c         2921 g         5261 t
ORIGIN      
        1 tttatttcgt tgattctcga ataatcaact ctgtttccac aataatcttt cttgaaaccg
       61 gtgctttgga attacttaat tgaagaatcg tctccactgc tagctctccc atccattcgg
      121 tataaacttt tacagtagaa agagagggcg ttacatattt cgctacactg acatcattga
      181 aacccatcac agaaatatct tctggtacca ctaaaccttt ttcttgaatc gctttcagtg
      241 cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt
      301 ttaaatactg cttcactgct tgatatcctg actcgacaga aaaatctgct tcgataattg
      361 gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa tgccataatt ctgggatctt
      421 ttagccgctg atggtttttc ttcgtatatt ctgttccagc aagcatagcg atatgttgcg
      481 ctttatggga gacaaaatgt tcaatgactt tttgaacact tgacttaaag tcgacaacta
      541 gagagtcgat tccttgagaa gtgccgtcag aatctaccaa aagcaaggca ttttgggatc
      601 ctttcatttc taataactga gcttcgtcga attttcctag tgcgatcgtt ccatctgtcg
      661 gaagatcact aatggaatcc cacgtttctt tcaataaagt aacgtttgct tcttctgcct
      721 ttttttcgat tcctagacga atagataaat aataaagatc ttcaagctct tccgtatcgt
      781 cgtaccactg gatcaggcga agcgtttgct tagtatttgc tgcattttta cgatgtttcg
      841 tataatttaa gcgttctgct gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa
      901 gatcaggatc atagttgaga actcttgaaa cagtagcagg agaaactcct gccaactgcg
      961 caatgtcttt aattgttgcc atttgtctct cctccttttt tacgtttata aaattgcagt
     1021 aaatcgttca aacgctgcct gtcctttttc atctcgcttg aatactccag cgtcttccag
     1081 tacacgtgca aaaacctgac ctacagattt ttgtacgaca tcggtgaccg tatctttcgt
     1141 caaatcagga tgttcttttt ttaattgttc tgcccacgga cgatgatatt cagccatctg
     1201 gttttcttct cctaataaga aatgctctac ttctactagt tctggtttca aacgaggtgg
     1261 taaaacagca agccccatta cttcgatcaa cccaatgttc tcctttttga tatgttggac
     1321 atctttatgt ggatgaaaaa tcccatcagg atgttcttct gaaacattgt tgtctcgaag
     1381 gacaaggtcc aactcaaagt aattgtccct tcttcgtgca attggcgtaa tcgtatggtg
     1441 tggtgtacca tctgctgaaa aagctctgat agatactgtt tcatccgaat atgctttcca
     1501 cttttccaat actgagttag ctgcttcaat caacaatttt ttctctgttg attgcaaacg
     1561 gatcacagac atcggccatt tcacgactcc tgcgatgact tgcggataat ctgctagttt
     1621 gataagtttt tctatcggtg cttttgccat aggaaattca tgacgtcctg cctgataatg
     1681 atcatgagaa agaatcgatc ctccaacaat cggcaagtca gcattcgaac caacaaagta
     1741 atgaggcaag acttcagtaa tccgaagcag tctagcaaat gtttcctttg tgattttcat
     1801 cgggcggtgt tgttctgata aaatgatcga atgttcattg taataagcat aaggagaata
     1861 ttggaatccc cagctttcac cgtctagatt cattcgaata atccggtgat tggtgcgggc
     1921 tggatagttc aagcgacctc tatatccttc gttttccatg caaagcatgc aggtaggata
     1981 gtctactttt accgcctcgc gttctgctgc gatttgtttt ggatcttttt ctggcttaga
     2041 taaattgatt gtgatttcca gttcaccata ctccgactct gccggaaatt tgatgttttt
     2101 tgcgatggct cttgttttga tgtagtcatt tttcttactt aaattaaaga aatactcagt
     2161 tgcttcctct gggcttttcg aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg
     2221 cggtgtgaga aaatccatta attgcgcttc aaaaatctct tgttctgctg gcaattctga
     2281 aagaacacca ttttctcttg ccttagcgac aagctgatcc aataagtcta cagattcttc
     2341 cttaactggt cttacttcaa tgctttctag agcatcttct cctaccatcc ctaaaagacg
     2401 attctgtaaa tataagcggt ccatttccat ccatccgcca gcttcgatcg caagtgtggc
     2461 aaaatcacaa atcgtttgac tgattgacat atctcattcc cctattcttc ataaccattt
     2521 ggatggctga catgccaatc ccatgctgtt ttgataattg ctttgatatc tgtgtattca
     2581 ggtttccaac ccagaacctc tctcgctttt tcacttgaag cgactaatcg gctaggatct
     2641 ccagctcttc ttggtgctat ttctgctgga atttcttttc ctgtcacttc tctagctgct
     2701 tctagcattt ccttgactga gtatcctttg ctgctgccta aattgaagaa gttgctttca
     2761 tttccttctt tcaagtattc caaagctaaa atatgtgcag caatcaaatc ttctacttga
     2821 acataatctc tgatacatgt tccatctggt gtatcataat cgtctccata aacagccaat
     2881 gcttttcttt gaccaagagc tacttgtaaa atgattggga caagatgcgt ttctggtgta
     2941 tgatcttctc cgatagatgc atctgctttt gctccggcta cattgaagta gcgcaaagcg
     3001 acatagcgca tgccatatgc ttggtcacac cacttcatca ttttttccat catcaattta
     3061 ctttcaccat acggattttt aggattagct ggtgtatctt ctgtgattgg agactctttt
     3121 ggttctccgt aagttgctgc agtagaagag aacacgattt tattcacatt gaactcgtgc
     3181 atgacttcaa gtaagatttg cataccgtat acgttgttgt tgaaatacat caatggtttt
     3241 tcgacagatt ctcctactaa agagcttgca gcaaaatgga ttactccttc aatcggttcc
     3301 ttttcaaata cggaacgtag aaattcttta tcccggatgt ctccttcata aaaatgagca
     3361 tctggatgta ctgcttgttt gtgacctgtg agcaaattat ctaccacagc tacttgatac
     3421 cccttttgaa ctagctggtc tacagcgtgc gaaccgatat aaccggctcc gcctagtact
     3481 aaaattgtca taaaaaacac tcctttgatt agtaaaattg tgtttgttga ttatatataa
     3541 tttaaaaaaa cgaagtttac ttttatggtg aaaacaaaca aagtaaaatt tcgtaaattc
     3601 atatggtaaa tttataataa aacatttagt aaacattttc aaggattttt tgcaaaggtt
     3661 tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt ccataataga tataacaaat
     3721 aataaatgag gtggattcaa tgaaaaaatt tgtatctgga atattagtag gaagtctggc
     3781 aacagccgct gccgttgctg gtttggtcgc ttctgtgaaa aaaacagtga tcgatccaat
     3841 tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgca aacgcgtttc
     3901 tcgttaataa ttatttaaaa acacgcatga agacttgtga aagaacagat aagtcatcat
     3961 gcgtattttt atttgtcagt caatataagt tgtgctattt tatctgcctg tctcagattc
     4021 catggatctg tataaggtct agctactgct gcagttagcc aatccacacc ttcaacaggt
     4081 attccatagc aataaaagtc agactggatt tcccctgaag catcaatcac gcgattcgta
     4141 cctcgctcaa catctatagc ttctgaatga taacttcctg tattatctga aaatgaaaaa
     4201 gggcgaacaa gtttgttttc cttcaaattt tttatgacag gattacttga acgagaaaag
     4261 cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt atcttgtaga
     4321 gaaataacat agcggttgtg cttttgctga atatcaaatg gcggttcgaa caaatgaaaa
     4381 atacccgcat ctatcagtgc tgctaattct ttcgtccgaa tgactggcgg accgatcgtt
     4441 aaaaagctat ttagatgagt aaaccatccc catagtattt ctttgtattc ctttgcagaa
     4501 aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc agtcttttaa tgcatcaaat
     4561 gttgaagcaa atgctcctgt gcaattgcct ttttccgctt cttttatctg cccttcaata
     4621 aatgctcggc tagcttctgc aaaggactca tcctgatagg taagaggaat ttctaaatac
     4681 gaccaatccc atctataagg gattagtgct ggatgctttc cgagacactc ctctgaagag
     4741 agggagagaa aatcatgctc aaaatttttc tttggaatcg gcaaatgata ttcttcgatg
     4801 attttcttgt aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa tgtttcgcca
     4861 gtcaaaaatc ccgactgatg ccattgctcc aggttttctt ttgttagtaa ccgtggccat
     4921 gccattgatc cgccttgctt ttggtttctt cctcgagggt aataagggag cccccttctt
     4981 gatccactgt atacaatcgg ttccttgcct gatggatgat agacaagttt tcctttttcg
     5041 ttctcgaagc gcccttctct tgatactgta aacaatccga cataatcaaa aaaacttaag
     5101 cctagacctc ttaaaaagac attttcttta ggcggtatct tcgaaagatc gacatctgca
     5161 ggattagaag gaagctgata cagtaaatca tttttctttg cgtagtctac ccacactctt
     5221 tcttcttctg tctcatcatt ttcccagtga ccgcttgcta aaacaagctg atcgatagaa
     5281 tagctttgct gatctgtctg taaaagataa ccatcttttc ttttgctgac tttttttact
     5341 aaagaataaa caaactgaat agaagctgga aattcttctt gcaactttga gaaaaaccag
     5401 cgttgataga gtccgtataa acatcgagta gattgctgat ctttttccag acgattgatt
     5461 tcttctataa ttagttgctt atttttggtt accgtctgtt cgatataatc tgtagcaggt
     5521 cctttactcc attgaaacaa gttgggtccc tgtgttacca atcctccact ggacaatgtt
     5581 tcatctgtaa aaagcgtcac ttgttgagaa acagaattca ttaataactc cggtggctga
     5641 ttcaaccgcc aaactcgacc tccaggacca ttgggatcga aaagaaaaag ttcaagagaa
     5701 tgtctgtcct gattattatg gagaatcctt tctgctatac ttaatcctct aggtccggca
     5761 ccaatgatcc ctattttcat ttgcatgcct ccatttcgat aaattttttc aataaaacag
     5821 catggttctg atgttcattt tttgcaccat aaacaaaagt aatgactgga tgttctttca
     5881 taagttgact gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc aattcttgct
     5941 ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt ttgcgtgctt
     6001 cattactagg tgcaatttct ttcaaccaca gatctaactg cgctttttct tttgaaattc
     6061 ctcgtggcca gaacctatcc acgagtattc tatagccatc ctctttttca gcatcaaggt
     6121 aaacacgttt taatttgatc atagttgttt ccttctttct gccttttcgc ttagcttata
     6181 tatgtagata atagcacaaa ataaaaaaaa tgaacggcac aactatccat tgcgccgttc
     6241 atgtgatctt ggtcaaaatc taatgattga ttcggttttt aaagctgtca tacaatcatc
     6301 tgttttatac ttttttactt ttgatttttc cagaccatcc gtcatatcca cccttaagta
     6361 aataaatctc ttgataacct gctttacgta atttatttgc tgtacggatg ctcaagcttt
     6421 tcttttgatc gtaaatatag acaggctggt cttttcgaat agatcctaat gaatctttca
     6481 aaacagtgta agggatgttt cttgcaccta aaatatgccc agcatcaaac acatcttttt
     6541 cgcgaacatc gattacttgt gcttttctca ttccttctcg aaattcttct tctgtcaaag
     6601 tcgttgctga acgcttggcc attactcgta gatagacttc ccaaaagatg attgcaagca
     6661 caatcaatag caaaatgata ttaattaccc ataaaaccat gtaatgcctc cgttttttta
     6721 agcaaatttc aaggctaatg aggcagcacc aatgacacca gcttcattac caagctctgc
     6781 taatttgatt tttgttgatt ctcttacttg cgggaaagta aattctttaa aatatttctc
     6841 tacacggcta cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca caatacttga
     6901 cggattcaat gtattcccca agttggcgca agctaaacct aagtagaagc atacgcgatc
     6961 aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat ctttacttgt
     7021 tacttcttca ccgttgtcta acattgcttt cagttttgaa tctcctgcgt attcttctgc
     7081 caagtaacga cctagacgta ccacaccagt agcgctagac acagtttcta aacatccgcg
     7141 tttaccgcaa gtacattcga acccttcagg atccacagtg atatggccga tctctccgcc
     7201 tgctccgctc acgccgtgaa tcaaatttcc gccagcaacg ataccaccgc caacacctgt
     7261 tcctagagta acgaatacaa cgtctggatc gttttctcca gcacctttcc atcgttcacc
     7321 taaagcagca acattcgcat cattatcgat agcgaatttg attcctgttc ctgcctcgat
     7381 cagttttttc acaaattgaa cttctgtcca gtttaagtta tacgcaccaa taaccgttcc
     7441 agcttcgctg tccacgcttc ctggggtacc catgccgatt ccgataaagt cttcggcttt
     7501 catattatat aaattcaaac gatggttgat tgattcaatg atttcaggaa cgatatgctt
     7561 accatcatcc aaaatattgg tatcgatgct ccatttttgt tgaatctctc cttctggtgt
     7621 taaaattgca aattttgctg ttgttccacc taaatcaatc ccaataatct tcttgtccat
     7681 tacaaatttc ctttctttcg gctttcttca attcggtgct ctctttttaa tatgcgccac
     7741 gctgtcatgt atgtatcatg atcaatcaaa cggctatcat acagattacg caattcgata
     7801 gtcattaatt caatgtcata gatgcgtgca cccacataga taaagatgcc aaattttttg
     7861 aacagctgct gtacatcata taagctctcc atctttaagc acttccttca tattatacaa
     7921 gtaaaccata tttttttaaa cccagtaaaa tacatattac aaaaagaaat acaaaaacta
     7981 gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt agacccaaaa
     8041 cattccctaa cactaatccg ccaattagcc caccgacatg tccccaaaca tctacagagg
     8101 tatccagaag accaaaaaca aatgtcaaca caataaataa aagaaaatgt ctgactagct
     8161 gctggatcgc tgggttatgt ttaaaatgaa accctaagat aaacaacgca ccgaacaaac
     8221 cgaatatcga agtactcgca ccaccagaca gaacattcgc ttcgttaaaa gcaaagcttg
     8281 ctgtatttcc cattatcccg ctgaacatat aaatcaagaa aaaacgccaa tggccataga
     8341 ttgcctcgat ttgctgcccc ataaaataca gtatcagtga gttcattacg aaatgcatca
     8401 gtccaaaatg gatgaagatt ggtgtcacta aacgccacca ttcattgaag tgaacaagat
     8461 aaggaccata cattccggca taataaggga tctcaagtcg cggaaataat tccatcatga
     8521 ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca taacggtcgt cttaaccatg
     8581 cacgcaattt tatttgctgt tgataattca tgaaaacctc ccatttcatt ttagtcaata
     8641 aacaattgtt ggatcttctg atcaaagctc tccggttgcc attgttcatt caattgttca
     8701 cgaaaaacaa ggctacatgt ttttttatga aaattattta aataccggtc gtaataacca
     8761 ccgccaaaac cgattcgata accggctcta ttgtagataa tacctggtac aatgaggaga
     8821 tcgatcatct ccggcaaata gagcttctct gattgtggct caagaacacc aaaactcgaa
     8881 cgtacaagaa cggactgttc atccatttca taaaacgcca tctgacgttt cggcaaagtt
     8941 ttaggtacaa ctattttctt ttgttctttt tctgctcgtt ggataaccgg ctgtgtatcc
     9001 agttcaaatt cattcgataa agtcgtccct atcacttttg ctgacttcca gtactcgctg
     9061 gcgaatagct gtttatagat tcgctgttct ttttcctttt tcttgccact tttggccaga
     9121 tttttcagct tttcgattcc tttccggcgt aatcttactt tcaattggtc caaaaactca
     9181 cccttccttc tctcataaaa tggtatagga aaaaaagaaa aatagcaatc gaactttcaa
     9241 ataaatgcat aaatagaata aaaagaagac ctagaaatga ttggccttcc ttttttaata
     9301 aatttcaact gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga aaatatggcg
     9361 caccaaaaag ggcaatcaaa attcctgtag gtacttcccc acttggtaga atcactctag
     9421 caaccatatc tgcactaagc agaaaaatgg aaccgaacaa accgctcaga acaaaggtcc
     9481 agttattttc tttacgtacg actagcttcg cagcatgagg agctagtagt cctacaaaag
     9541 ataaactacc tgtcaccgct acactaccgc ttgccaaagc aacagctaac aacaaaagga
     9601 ttttttgact tttagccacg ttaaggccta atccttgcgc gcgttcttga ccaaatgata
     9661 gcaatccaat cctctgtgca taaagtcctc ctagcggaat taagataacc gcccatggaa
     9721 gcaatgccca aacataaggc cagctcgcac cccagattga tcccgctaac catgcattca
     9781 caaatccata attttcctta gataattgga tcgtgcatag caatgttaat gcagaaaggc
     9841 cggcattaac ggcaataccg gacaataaga ttcgattcat acccaaaaag ccatttcgat
     9901 cataggccgc aagatagaca cagcaagcag ctaaaacacc acctagaaag gctacaacta
     9961 gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc actaaaaatc
    10021 ctgctccggc attaatgccc aatatactag aatctgccaa ttcatttctt gttactcctt
    10081 gcaagatata accagaaacg ccaagacaag ctcctgctaa taaactgatc agtaatcgag
    10141 gaactcgaaa ttctctaaca atcagcgcca aagaagatga ttctttccct atgaacgtgg
    10201 caaaaagctg atccaggtaa ataaaaggcg taccaatcat taaactacct atagacagga
    10261 tgacaagtac aaaaagcaag aaatagaacg tccaatatat ttttttcacc gttgttccct
    10321 ccttacttgt atcagtaaaa acggaacgcc aatcaatgca atgactaatc caaaaggtgt
    10381 ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc aacataacca atccaccgcc
    10441 aactaaaaaa gagacaggta gaacataacg atagtccgtt ccaaccattc cgcgaacaac
    10501 tggcggaacc aacagcccaa taaatgaaac aggtccgact aacgaaacgg caatggctgc
    10561 taacactgca actagacaga aaacaatcaa ccgcgttcgt ttaggatgac ttccaagagc
    10621 taatgcatgt gtttcactca aatgaatcaa agatattgct tttcctaatg aaaaagaaac
    10681 caaaacagca agtatatata aaggaaacaa tactgctaat tgtttccatg tgatattggc
    10741 agctccgcct acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt gggtaagtgc
    10801 ttggctgata gcagtaaaaa aagaactaat agctacgcca gctaaaatca gctgtaaagg
    10861 attcatgccc attttcgaaa actgcaaaaa accaaatacg agcaaagtta cagcaaaaga
    10921 acctgccaat gacattagaa acacagaaaa atcagaagct ttaggccaaa aagcaaaact
    10981 aagggccatt gccaacgaag cacccgcgtt gattcctagt aagcctgaat ctgctaaagg
    11041 atttcttgtc actccttgca tcaatgcacc gctcaatgca aaacaactcc ctacaagaaa
    11101 tgctcccgcc acacgaggca aacgaatatt tcgtatgatt tggtgacttt gttttgatct
    11161 atcaaaatca aacaacgcct gccaaaaatc tgcttttccc acagtcgctg cgccatttga
    11221 aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga gtctattttt
    11281 cttcatagtc ctcataattg atcctcctgc tcttttggca acaaatcata ggttagaaaa
    11341 actggtttat ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa tatttttttc
    11401 atacactctt ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt accgtttttc
    11461 attgctaata aataatctga aaaacgtgaa gcatgattaa tgtcatgaat cgacatcaag
    11521 atcgttgttt gtttcatttg attgatttca gtcaataact gtaggatctc taattggtgt
    11581 gccggatcta aaaatgttgt cggttcatcc aagatcaaga tatctgtttc ctgagcaagc
    11641 gccatagcga tccagacacg ctgggtttgt ccaccagaca gcgttgcgat atcccgcata
    11701 cgaaatccag ctagacctgt agcatccaac gcccactcga ttgcttcatg atcttgcgtg
    11761 tccatgccac tgaacagttt ttgataaggg tatcgtccgt aagatactac ttcttctact
    11821 gtcatacctg tagggtgttc actagtttgt gctaataatg taattttttt tgcaagttct
    11881 tttgaactgt atgtagagat atcagagtta ttgacaatga tctttcctga tttcacaggt
    11941 agtatacggg taaaactttt caataaagta gattttccac ttccatttgg cccaatcaaa
    12001 ctagtgattt tcccttcagg aatcgtaaca gacatgtttt caatgatttt tttgttttcg
    12061 tagccggctg caatgtcagc tgctcttaat ctcgtcattt cacccatcct ttcgctgaaa
    12121 atcattctca attatgacgc tgtttcaaca tttcgtcaag cgctactttc atatctatag
    12181 ccaaatgaag cggttgacag cgttcgaaat atcaggaata ataaccaatg agaaagattc
    12241 tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa agtattcaca ttatctagca
    12301 tattgctgct ggctggagta ctgagcgctt gctccccctc ttcagataaa gaaacagcta
    12361 caactgccaa ttcgacgact tcatccaata gtactcatac agtcacagat acacttggac
    12421 ataaagtaga gattccaaac gcgcccaaac ggatcatcgg cagttactta gaagactatc
    12481 tgattgcact aggggaaaaa cctatcgccc agtggactgt tggcagtggt tccattcaag
    12541 attatctgca agatgattta aaggatgtcc caacgatttc ttatgacctt ccttatgaga
    12601 aagtgttaag ctttgagcct gatttgctgt tgatttcttc ttccgctact gtcgaaggcg
    12661 ggaaatacga acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac ggaacagatg
    12721 tcacatggga agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa aaagaggcag
    12781 aaaaaatcat ttccgattat caaaaatcca tcaagaaaac tcgagaagaa ctagcagata
    12841 aaatcgagaa caagacagct gcagtgttgt gggtgaccaa caactcagcc tttatggtca
    12901 gcaaaaatcg gtcaagtgga agaatcgtat acgacgatct ggaatttggt gttcctgatc
    12961 tagtggaaga ggtctccaaa aaagccactt ctgactggtc tgctgtttcc tctgaaaagc
    13021 tagctgaatt agatgctgac tacatcatcc ttgtgaacag tgacaaagat gctgccatgt
    13081 tcaatgaacc aagctggaag aatcttaaag cagtgacaga caatcatgta ctgcaattcg
    13141 gacctgaatc tagttggcta tataacggac ctattgccag tcaggaaatg gtcaatgata
    13201 tcaaagaaaa actaaattga ttttaactaa aaaaagaatc ttcacgatta gtcgtgagat
    13261 gctttttttc ttattaacaa actagacgca tagattttat tcttcttttg aagcaaccct
    13321 attttttact ctttcccatt tttgaaaacg atgggcatat agattttctt gatccgttat
    13381 cccctcatac tcctctataa gggcgaagtc tgaaaaatca atattcccaa tatacgtatc
    13441 gccttcaaac gtgctatcga tcaatgttcg ccagatccga ccagtgtaag gaagaagttc
    13501 ttgaaaaatc cgactaccac ccgaaacata gatatcttct gagatcgttt gactatacga
    13561 caaaagttca tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa aatttggttg
    13621 cgttgtcaac acgatgatat ggcgataagg aagtgaaaga tcccccattc cttcataggt
    13681 ctttcttccc atcactaaaa ttcgatccat tgtatgttcg cgaaagaaac gcatatcatt
    13741 tggcaatcgc catggtaaca aaccattttt gccgatcaga ccatttttat cttgtgccca
    13801 catggaaata aacatattat ccgctcctta tgattttaga tgttttgaga taaaaaaagt
    13861 ctcaattctt tgaccagcaa ggaattgaga actttaaaaa cattattttg tttcacggtg
    13921 caaagtaact ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat caggattgtt
    13981 acgtttgttt ttgcttgtta gatagttacg ttctttacaa gaagtacact ctaaagtgat
    14041 gtttacgcgc atgtttttcc ctcccatttt tacaagaatc ctctaagatg actctcagac
    14101 ttgattatct taccatgatt ttttctagat tgctagtgtt tttcacgatt ttgttaagta
    14161 aaactacttg acgataaata caacggtttt acttctcaaa caaaatcttt ttctttttgt
    14221 ataaataccc ttacacttgt ttatatagat tatgtttttt aataaagggg gatactaata
    14281 tgccatggaa catgaaggac tttcctgcat caatgaaaaa cttagataaa ttgacaagaa
    14341 aaaaagcaat cgacatcgca aatgcattat tagatgaagg gtaccctgat aaccgtgcga
    14401 tcccaattgc cattgatcaa gcaaaaaaat ggtatgaaga tgctagtgaa tcagaaaaac
    14461 gaactttcga aaaagaaaaa aatccaagca agacggatga acacgatacg aatcctcgtg
    14521 ctggaaaact gcttgattca gatgtgatcg tagaatatga ggaagaacaa tggatcgtga
    14581 aatctaaagg ggcaaaaaag gcaagcaacc attttgatac aaaaaaagaa gcaatcgaaa
    14641 aaggaaagca agtagcacaa aacaaagaat caactttgtt catttataaa aaagatggga
    14701 caaaagaaaa agaaatcgat tattgaacat atgtaaaggg gttgtgacga aaatcgtgtc
    14761 acaaccccct tatgttcgaa acaatggtgt tcctctgcca actccgaacc ataagtcata
    14821 atatctataa aacgggaaga gctgtttttt gaaattgttt cttatcactc agtgttaaac
    14881 ggtaggagaa aacctctttt ttattgatat ttgattttca tcaaacagtc acatagtcga
    14941 ttgcttctgc ttgcggaatc gtttcatcta caaagtagac tttataccat gtcaagaaac
    15001 tgaagattgt ccagattttt ctttgctctt ctttacgtcc ttcgtgatgg tcatctagca
    15061 acttcatgat tttttcttga tcaaaaaatt cttttgcaaa atctgcttcg aataatgctt
    15121 tgacttgttc gtagccgtgt tcttctttca accaagcttt gattggcaca gggaatccta
    15181 atttcacacg attcgaccat tcttccggaa gatgtttgtt cgcagcttgt ctgaaaatat
    15241 cttttgtgtt gttttgattc aataaaaact tacttggtat ttgttgagcc aatttcatga
    15301 cttcgatatc caataaaggt actcggactt ccaaagaact tgccatagaa agtttatctg
    15361 cctttaacaa gatatctttt ggcatccatt gatgaagatc gacatattgc atcttattga
    15421 cttcatcttt gattccttct gttttctcgt aatgaacagt catgatttca tccacagatg
    15481 gtgctgtttg atattttggt gttaacactt ctgatgcttc tgattcttcg aacactcgag
    15541 catgaccgat aaaatattct tttgcaggag caagtgattc atataaatgg atccgtccat
    15601 ggaaattttt catttttccg attttacgtc ctaaattata gcgtgttcct tttggtaatt
    15661 ttttcaatcc ttgagcaatc acacggatga attttgaatt cgtatggaat ccatacgttt
    15721 ggtatcccgc aaacagttca tccgcccctt ctccagattg gactacccgc acactttgtg
    15781 aagcaagatt tgctaaaaag tacaaaggta cgcaagaagg atttgaatca ggttcatcta
    15841 agtgatactg gatcaaaggg aatgctttga acgacatgtc tccgtcgatc actgctgctg
    15901 tattatctaa gtccaatagt tcggttagtt ttctggcttc aatcgcttca ttataggttt
    15961 tatcatcaaa cccgattgag aaagaatgat ctggtttcaa tacagaggtt acataactgg
    16021 aatcaacccc gctggataag aaagaaccaa cttctacatc actgaccgta tgtgcttcaa
    16081 tagaagcttg aacggtttcg tcgatttttt cgatccattc ttgtcgggaa tgcgtttctt
    16141 ttgtttcaaa atctgcatcc caatattgct gaatgtctag ttttccatct ttatacgtat
    16201 aataatggcc ttcaggcaaa cgatagacat ctttgaagaa tgtttcgccg tttaatggag
    16261 aatattggaa agtcatataa ggtttcaacg cttctttgtt caattctttg ttaaaatcag
    16321 gatgaggcag aaaacttttg atttctgaac caaacataaa tgttccgttc atttctgcat
    16381 aataatatgg tttgatccca aagtggtctc ttgcaccgaa taactcattc ttttcattat
    16441 cccaaatagc aaaagcaaac attccgcgga tcttttgcaa taattctgtt ccccattctt
    16501 cgtaaccatg caaaaggact tctgtatctg catgtgtttg aaaaacgtgg ccagctgcaa
    16561 tcaattcttc tctcaaaggc tggtaattat agatttcgcc attaaaaata atgatctttg
    16621 tccgatcttc attgtaaata ggctgagtac cgccctctaa gtcaataata cttaatcttc
    16681 tgaatcctaa agcaacatgt ttatcgatat attctcctga actattaggt ccacggtgaa
    16741 cgattcgatc catcattgta ttaataattg tttttttgtt gtccttgtcg ttaacaaaac
    16801 ctacaatacc gcacatttaa ctttcacatc ctaatataaa atta
//
LOCUS       DAYYAG010000057        16749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000057 DAYYAG010000000
VERSION     DAYYAG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.057"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACHARQ_001611"
     CDS             complement(<1..283)
                     /locus_tag="ACHARQ_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8410020.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYR"
     gene            complement(285..482)
                     /locus_tag="ACHARQ_001612"
     CDS             complement(285..482)
                     /locus_tag="ACHARQ_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8410021.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(876..2603)
                     /locus_tag="ACHARQ_001613"
     CDS             complement(876..2603)
                     /locus_tag="ACHARQ_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8410022.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2596..3789)
                     /locus_tag="ACHARQ_001614"
     CDS             complement(2596..3789)
                     /locus_tag="ACHARQ_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8410023.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3976..4620)
                     /locus_tag="ACHARQ_001615"
     CDS             complement(3976..4620)
                     /locus_tag="ACHARQ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8410024.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4714..4848)
                     /locus_tag="ACHARQ_001616"
     CDS             complement(4714..4848)
                     /locus_tag="ACHARQ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8410025.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4850..6982)
                     /gene="feoB"
                     /locus_tag="ACHARQ_001617"
     CDS             complement(4850..6982)
                     /gene="feoB"
                     /locus_tag="ACHARQ_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8410026.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6979..7452)
                     /locus_tag="ACHARQ_001618"
     CDS             complement(6979..7452)
                     /locus_tag="ACHARQ_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8410027.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7853..8077
                     /gene="nrdH"
                     /locus_tag="ACHARQ_001619"
     CDS             7853..8077
                     /gene="nrdH"
                     /locus_tag="ACHARQ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8410028.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8236..10395
                     /gene="nrdE"
                     /locus_tag="ACHARQ_001620"
     CDS             8236..10395
                     /gene="nrdE"
                     /locus_tag="ACHARQ_001620"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8410029.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10445..11410
                     /gene="nrdF"
                     /locus_tag="ACHARQ_001621"
     CDS             10445..11410
                     /gene="nrdF"
                     /locus_tag="ACHARQ_001621"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8410030.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11499..12638)
                     /locus_tag="ACHARQ_001622"
     CDS             complement(11499..12638)
                     /locus_tag="ACHARQ_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8410031.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12947..13660)
                     /locus_tag="ACHARQ_001623"
     CDS             complement(12947..13660)
                     /locus_tag="ACHARQ_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8410032.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13914..14615)
                     /gene="nagB"
                     /locus_tag="ACHARQ_001624"
     CDS             complement(13914..14615)
                     /gene="nagB"
                     /locus_tag="ACHARQ_001624"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8410033.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14849..16381
                     /locus_tag="ACHARQ_001625"
     CDS             14849..16381
                     /locus_tag="ACHARQ_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8410034.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16572..>16749)
                     /locus_tag="ACHARQ_001626"
     CDS             complement(16572..>16749)
                     /locus_tag="ACHARQ_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8410035.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5200 a         3448 c         2885 g         5216 t
ORIGIN      
        1 ctcgatataa accatttatt ccagcagttc ttccaatttc actcaaattt cttattaatt
       61 cctcttgata ttcagtgaat cttattttat tagtataagc agtaaaatat agataattca
      121 ccaatctatt tattaaagga ctgggatcta attcatattt ttgtttttct aattttaaat
      181 ataccttttg taaaacctca agaatatcag aatatttagg atctttactt atagatatat
      241 cattatataa ttcatgaaca atttgcttag catttttttt cattttagca cttccctgga
      301 attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt tgccttggcc
      361 caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata ttttccacta
      421 tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt taaatgtttc
      481 ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt cattaaattt
      541 caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa tttaatcact
      601 tttaagtata taaactatgt atattcttta atattttttg agaatgcact tcgtacataa
      661 taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact cgcacccata
      721 aattcataat agtttgtcgg cgtctttgaa cgtttcgaag atgagctacg cattctcttt
      781 ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat atttgatcca
      841 attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct ttcgactaat
      901 tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc attgttgatt
      961 tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa tactttgtaa
     1021 gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg ctcttcgctt
     1081 cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa catccaatga
     1141 ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc tagtgcaacg
     1201 gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc atatttttgg
     1261 atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat gattttctta
     1321 cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg cactgctaaa
     1381 ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt atattgtgca
     1441 tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga ctcgccattt
     1501 gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc atagcccatt
     1561 tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca ttcaacataa
     1621 gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc aatcgcacct
     1681 aagatcacta ctactacagc accaatgatc agtccttgac gttttttact tttctgtgtt
     1741 gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa acggtcaatg
     1801 atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt
     1861 tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga ggcaataacg
     1921 accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt acttttagct
     1981 aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga atcagaagct
     2041 tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc
     2101 agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa aaatgcaact
     2161 gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat cggtttgata
     2221 atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc aatgatgatc
     2281 gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt ccatagacct
     2341 tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg acgtttctta
     2401 gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg aatcactaac
     2461 agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg
     2521 ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc cactggtaat
     2581 tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta atgcttcaat
     2641 cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg caagtggagt
     2701 cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc caatcaccgt
     2761 cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg
     2821 atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca gcatgctgac
     2881 ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga tattcgtcgt
     2941 caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga cttcttccac
     3001 aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg ttccgatctg
     3061 catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag cttcattcaa
     3121 atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca gtaattcgtc
     3181 ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca tcagcaagat
     3241 ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc caccagataa
     3301 ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg ctttttctgc
     3361 tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt ctgtattttc
     3421 caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga ttttatgacg
     3481 acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat caatatagat
     3541 actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg atttcccact
     3601 cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat tcacgtcata
     3661 cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt gatcattcat
     3721 catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt ttacttctac
     3781 ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga aaaaagtgac
     3841 cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa
     3901 actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt aatatttttt
     3961 taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg
     4021 ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa atttagcata
     4081 ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg tggcgccagt
     4141 cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt tttctgataa
     4201 agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt gattgaccag
     4261 ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca cacttgccat
     4321 gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata ggaaatcttt
     4381 tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca tgattccaag
     4441 atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt acgtactggc
     4501 aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa cgatacgttc
     4561 ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct ttccagacat
     4621 tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc aaaataagca
     4681 aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac
     4741 tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag tatagacaat
     4801 tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc tattcctctc
     4861 cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg aacaaaacat
     4921 aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc tgtccaccct
     4981 ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa ccgcattgat
     5041 agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata gccccaattg
     5101 ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct gctgcgggag
     5161 tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact tccgcatgac
     5221 caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct gtaatcgctg
     5281 cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc
     5341 cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa ttataagaag
     5401 agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt ttaacaaaag
     5461 acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg
     5521 gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt ttcaagctga
     5581 ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct acccaagatt
     5641 gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca gaacaaggca
     5701 tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg
     5761 ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag gattttcctg
     5821 aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc atatatccgc
     5881 agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga aatcctaaca
     5941 ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat cccgctactt
     6001 gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat
     6061 tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag atcagccaca
     6121 tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga tcgattttgt
     6181 cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat agttgggtaa
     6241 taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat agtttctctg
     6301 ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg taatcctctc
     6361 ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg ccgctttgtt
     6421 ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca tatgtacagg
     6481 gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt agtttttcga
     6541 ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca taggaaagct
     6601 tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc agaccaatga
     6661 tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt tctaaattgg
     6721 tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa tctcttgcaa
     6781 tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa
     6841 tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc ggccaattcc
     6901 ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg ctgtttggat
     6961 ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta gttcggcctc
     7021 tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat ctcccagagg
     7081 ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg
     7141 cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg cttttaattg
     7201 atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga tttgacctag
     7261 tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat tgttgcctga
     7321 taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca aatggcgttt
     7381 tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc
     7441 taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa tcattcatta
     7501 tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca catagaatac
     7561 tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct ttagtattga
     7621 tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt ctctattaaa
     7681 tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca caaaacttag
     7741 tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt ttactaagag
     7801 ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca ccatgaacgt
     7861 aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt ttttaacaga
     7921 aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag tagactggtt
     7981 gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa cagttatcgg
     8041 attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga atcaaaacgt
     8101 gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact tccaaatcct
     8161 tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga
     8221 aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt caagctgaat
     8281 aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa agaagcttta
     8341 gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttccatc tattatggaa
     8401 aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca attgtattca
     8461 ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc tttcaaatct
     8521 tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga cggttccgct
     8581 tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc gaacggggat
     8641 gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca gccagctaca
     8701 ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg tttcttgact
     8761 caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt gcaattgtcc
     8821 cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg cgctcctatc
     8881 aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt cgaagatagt
     8941 ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta tttgaacgtt
     9001 ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt
     9061 cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga attgacacgt
     9121 aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata tggtgtccca
     9181 ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc aaacattcgc
     9241 aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca acaagaatct
     9301 ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat cgatggcaag
     9361 atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc tgtgattaac
     9421 ggcaaacagg aatatgaagt attaggaaca gatatctcat gtaacttagg ctcgacaaat
     9481 atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca
     9541 ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca aaacggaaat
     9601 aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct tgcaaaagaa
     9661 caaatggaat atggctcaga ggattcatta gactttacaa atatttactt catgctgttg
     9721 aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca agttttccat
     9781 aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt aactggagaa
     9841 ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat cccaactgct
     9901 gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca tcaaaatcgc
     9961 ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc aagtattcat
    10021 cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat ttattaccca
    10081 gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga tatggacatg
    10141 cgtaaagtaa tcgatgttta tgcaacggca caacaacacg tcgatcaagg catgagtttg
    10201 actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac aacaacgaag
    10261 caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg aatcaaatcc
    10321 atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa
    10381 agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt aaaggagcat
    10441 tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag atatcattga
    10501 taaatccact tgggagaagc tgacagagca attttggcta gatacacgta tcccactttc
    10561 aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag taggacatgt
    10621 atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg atcagttgcg
    10681 taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat tcatggaatc
    10741 cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa aagaaatcga
    10801 agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa
    10861 tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct ttttagaaac
    10921 attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca acaaattggc
    10981 taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg gaacctacat
    11041 cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag aaacgttaaa
    11101 agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt atacagaaga
    11161 attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct ataatgccaa
    11221 caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca atgatgtcaa
    11281 cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct tctcacaagt
    11341 cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt atttgattgg
    11401 attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag agtttagcgt
    11461 acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt ttcttccagc
    11521 acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa atagattttc
    11581 cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat cagcatgcct
    11641 aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa aggatggata
    11701 ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg ttggatcact
    11761 aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc gacaatgatt
    11821 gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat tccgcaaatg
    11881 accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa gctcattaca
    11941 ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt ttcgtttaat
    12001 tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg agacaacgca
    12061 tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac ggtaaataac
    12121 aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga taaactgact
    12181 aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg ttcttccaaa
    12241 ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct tgtttcatct
    12301 gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg aggcactaca
    12361 ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac
    12421 actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat ataatagata
    12481 aaaccagcaa caagcaatgg taaaaaaatt gccgaaagca ttttcaatat tggactaaaa
    12541 atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag ctccactgtc
    12601 cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca aggtcctttg
    12661 atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt tgcttaaaaa
    12721 aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa aacacggttt
    12781 ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg ttcaaaagct
    12841 tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt tatttggctg
    12901 ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat tgcgcaatac
    12961 tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc acatcggcat
    13021 aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg tagtcatcaa
    13081 cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca aaataatcgg
    13141 taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag
    13201 gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga
    13261 tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt gcccataaag
    13321 aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta
    13381 agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc
    13441 cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc tcaacttgga
    13501 tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata cctgctggac
    13561 gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg atccctaaac
    13621 gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa
    13681 tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag
    13741 gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc aatcagctga
    13801 ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt tgtcttctct
    13861 aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga aaattacaac
    13921 ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg caatacactt
    13981 gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc agctttgctt
    14041 tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat tcccattgaa
    14101 acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc attgattgtt
    14161 gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa
    14221 ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg tgcatcgatg
    14281 atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc tggtacaaac
    14341 gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg gtagctttgt
    14401 tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat ttcactgaaa
    14461 tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc tgttgccaat
    14521 cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc tttttttccg
    14581 ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt atattttggt
    14641 atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga ccaaattgca
    14701 tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa gatggttcaa
    14761 tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg ctttatgtct
    14821 gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca acattaccca
    14881 gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg ctggcgaaca
    14941 tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa tcatcactgg
    15001 tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca aaggttacct
    15061 cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa aaactgcgta
    15121 cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat atagcgagac
    15181 cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag accaaggagt
    15241 gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga tcgatgtctt
    15301 aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg tgattttagc
    15361 aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca atcacttaga
    15421 tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt
    15481 gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag acatcgagtt
    15541 tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa
    15601 tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc
    15661 ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca
    15721 attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg tctttgattt
    15781 tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag tcatcggata
    15841 tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa atattgccat
    15901 tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg gtgagattga
    15961 aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact tttcacaaga
    16021 tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc aatttccaaa
    16081 agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta atcaattagc
    16141 aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt
    16201 gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata tcgatcaaga
    16261 aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa tcatcgtctc
    16321 tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg aaccaaaata
    16381 aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc acaactcctt
    16441 cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa
    16501 tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa
    16561 cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga
    16621 aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac
    16681 tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac
    16741 ataaataca
//
LOCUS       DAYYAG010000058        16725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000058 DAYYAG010000000
VERSION     DAYYAG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.058"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..656)
                     /locus_tag="ACHARQ_001627"
     CDS             complement(<1..656)
                     /locus_tag="ACHARQ_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8410036.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     S"
     gene            831..2255
                     /locus_tag="ACHARQ_001628"
     CDS             831..2255
                     /locus_tag="ACHARQ_001628"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8410037.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2554..3261
                     /locus_tag="ACHARQ_001629"
     CDS             2554..3261
                     /locus_tag="ACHARQ_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8410038.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3248..3571
                     /locus_tag="ACHARQ_001630"
     CDS             3248..3571
                     /locus_tag="ACHARQ_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8410039.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3660..4697)
                     /locus_tag="ACHARQ_001631"
     CDS             complement(3660..4697)
                     /locus_tag="ACHARQ_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8410040.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5018..6001)
                     /locus_tag="ACHARQ_001632"
     CDS             complement(5018..6001)
                     /locus_tag="ACHARQ_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8410041.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6416..7186)
                     /locus_tag="ACHARQ_001633"
     CDS             complement(6416..7186)
                     /locus_tag="ACHARQ_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8410042.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7179..8114)
                     /locus_tag="ACHARQ_001634"
     CDS             complement(7179..8114)
                     /locus_tag="ACHARQ_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410043.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8430..10079)
                     /locus_tag="ACHARQ_001635"
     CDS             complement(8430..10079)
                     /locus_tag="ACHARQ_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8410044.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10196..12799)
                     /locus_tag="ACHARQ_001636"
     CDS             complement(10196..12799)
                     /locus_tag="ACHARQ_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8410045.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12811..13860)
                     /locus_tag="ACHARQ_001637"
     CDS             complement(12811..13860)
                     /locus_tag="ACHARQ_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8410046.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13886..14200)
                     /locus_tag="ACHARQ_001638"
     CDS             complement(13886..14200)
                     /locus_tag="ACHARQ_001638"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8410047.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14216..14662)
                     /locus_tag="ACHARQ_001639"
     CDS             complement(14216..14662)
                     /locus_tag="ACHARQ_001639"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8410048.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14663..16585)
                     /locus_tag="ACHARQ_001640"
     CDS             complement(14663..16585)
                     /locus_tag="ACHARQ_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8410049.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4956 a         3399 c         2755 g         5615 t
ORIGIN      
        1 gaatactcga aaggtttacc agacgataga gcaacaactt tgtctatctc aatcataaat
       61 tcacctggat ctatttttaa tatttttgcg tcaaattcat tcgcagtatc tcctttgata
      121 cgtatgtgcg aagtctttat ttccagaccc agttcatttg tgatataagc atatacagat
      181 ttctttaaat gttttggttc caagcctggt atgactgcta atggcatgaa tgtttgctca
      241 atcgagtatg gttgacaatc aataaaacgg cttcgtacaa tattatatac atagtctttt
      301 acactgatac ttaatttatc agctactttt ttcgatggaa gttcaattga gaaagtatgg
      361 atttctgaat ctacgtgttc ttcaccaacc acgctaaaca aaccttcgtt tggaccaaga
      421 actcgtgagt tatcaattgg tgttctgacg acttcagtcc catacccact tctacttcgc
      481 aaaactcctt cggaaacaag taagtctacg cctttcttta ctgttaactt gcttgtatga
      541 taaataactg ccaattcatc tcctgtagga agtttctcac caattttata ctctccattt
      601 acaatttttt tctttatatc taagtaaatt tccatatact tcgcaatttt tttcaatggt
      661 gaccctccaa tcaaaaacat atcttcatct atactttaac ataaataaac cgataaaatc
      721 cacatcaaaa ttatacacac aataaaaaag atatatatat ttaaacaaaa ctatttactt
      781 taagttgtaa gcggtgttat cattaagtgg aaacaggagg taaaaaaatt atgacaacta
      841 tgaaaaagct aacttttcca gatgattttt ggtggggttc cgcttggtca gctgagcaag
      901 cagaaggaaa aggagaaaca ggaaaagcag agactatctg ggatcgctgg tttaaagaac
      961 aaccttaccg gttttatcaa cgaatcgatt cctctattac aaccgatcac atccatcaat
     1021 ataaagaaga tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc gtttctattt
     1081 cttgggcacg aatgtttcca aatggcggta ccggaaaagt aaatcccaaa gccatttctt
     1141 tctatcgaaa attattttcc gaaatgaata ataacggaat caaagtattt gccaacttat
     1201 atcattttga catgcctgct actcttcaag ataaaggagg atgggaatca cgtgaagtag
     1261 ttgatgctta tgttcattac gctgaaactt gttttaaaga atttggtgac ttagtttgtc
     1321 attggtttac cttcaatgaa ccacttgggc ctattttagg tacttatctt gaagattttc
     1381 attatccaaa cctcattgac tttaaacgcg gagcgcaagc agcattcttc actatacttg
     1441 cacacgcaaa agcaatacaa gtatttaaag aactagctct tcctagtaaa ataggcgtta
     1501 ttctaaattt gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg aaagcagcag
     1561 aaatcgctga tttattttat acgcggagct ttttagatcc tatggttaaa gggaattttt
     1621 ctaaaaagct tatcctgtta ttacaagaat ataatcagct tcctgaagat tatacgaaag
     1681 aagatcttgt atgtatctac gaaaataccg ctcaaattct tggactaaac tattacgagc
     1741 cacgtcgcgt caaatctcgt tcttctatgg tggatacatc tagtccattt ttaccagagt
     1801 ggtttttcga accacatatc atgcctggac gacgaatgaa cgaataccgg ggatgggaaa
     1861 tttacgaaaa aggaatttat gatttgtgca tggatatcaa gaaccattat ggaaacatcg
     1921 aagcatttat ctcggagaac ggaatgggcg ttgcggatga agaacgtttc atggatgata
     1981 cagggcaaat aattgatgat tatcggattg agtatatcaa agatcatctt gcttacttat
     2041 ggaaggcaat aaatgaagga tgtaacatta aggggtatca tctttggaca tttattgatt
     2101 gctggtcatg gatcaacgcc tataaaaacc gatatgggct ggtttcactc gaccttacta
     2161 ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa gcaattaagt gatacaaatg
     2221 gatttagata cgatgtagac ttgttagttg aataatattc taaaattcca cttgaagtga
     2281 acacattaga aaacaagaat aatgagaaat accaataaca aatacttttt cttcatactt
     2341 catttatact tgtcagtata agagactgtg acaaatgtag tgatcctcag aagttaggtc
     2401 tggaaatcta acttgagaag atttttttaa actacgtcca gtctttttta tttgtttaaa
     2461 attgaagttg acaagtcttc attaaaaatt tagaatattc acaagtttga cgacaattat
     2521 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
     2581 gcgatcaaag acacgttacc taccgttttt ggttatatcg gtattggact tgcatttggt
     2641 atcgttggga aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc cctgctgatc
     2701 tatgctggtt ctgcccaatt tatcacggtc agcatgcttg ctagccatag tccactgctc
     2761 tccatcgttc tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt
     2821 gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca
     2881 gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt
     2941 cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt
     3001 ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc
     3061 gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa
     3121 cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt
     3181 atcccaagta acttagtgat tttagttgta acactgattg ggtgcggatt gggggtgtgg
     3241 atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac
     3301 ttggctttct aggatcctac catttgtctt actgaaaaaa ttcgatttgc cgcaaccgtt
     3361 gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct
     3421 atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt
     3481 tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggtaggaat
     3541 tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta
     3601 ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct
     3661 tatccgttga acggaccact aacaacgact tttcctaata tatgtcctgt ttctacgtct
     3721 ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta
     3781 ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt
     3841 tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat
     3901 tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt
     3961 ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc gggtaatatc gaataataca
     4021 gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga
     4081 taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca
     4141 gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc
     4201 ccagctccgt tgatcactaa gatttttttc ccgcggttgg cattttcttc tggagtcaga
     4261 ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcggct
     4321 tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac cagttggtat
     4381 tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc gccgacagaa
     4441 aatttttgca cttcatcacc tacagcaaca actttcccga ctccatcaaa ccctaagata
     4501 cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat
     4561 actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca
     4621 tcaataaagc tttttggatc atcgatcgac aacccttcat aaaaaccgac taccttcatt
     4681 aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag
     4741 ttttcatttc aatatatata gtatgctatt tgttccactt ttattcatca taattattct
     4801 cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg acaagacttt
     4861 tgtcacaacc tcttttaaac ggtgttcaat caactgaccc tcggtattcg ctcaaaaaac
     4921 gacaaaatat gaggagctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt
     4981 tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg aaaagcgttg
     5041 gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc atcagtagat ttcgtattca
     5101 tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt ttaaagaaac
     5161 gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc agatctagtt
     5221 gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcggttct ttttcaaatg
     5281 cataaggatt cttgaaaatc ccccggccaa tcatgatgcc atctacgcca tatttttcgg
     5341 ccaattcttc tcctgtctgg cgatctggga tatctccgtt gatcgtgatc agtgtttgcg
     5401 gagcgatttg gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt gcatctactt
     5461 tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct tgatgaagta
     5521 ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc gtcttgacgc
     5581 tgacaggcaa accgccagct ttagccgctt caatcaattg tgccgctact tctgggcgaa
     5641 ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag cccatgttga
     5701 tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta aaaaattctg
     5761 gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag gtcaatcttc
     5821 ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta aactcagtaa
     5881 agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca tctgttacat
     5941 cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca
     6001 tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc aaaaacattg
     6061 tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat gtttctgatt
     6121 gagcaaaaaa agactgtgac aaaaatctta tcacaattcc ttttccggaa taaatggtgt
     6181 tcaaaagctt gcatctgcgg taataagtcc aaccaaacga aaaataggag gagctatttt
     6241 tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct ccactaaacg
     6301 gtgttcaatc agctgaccct cggtattagc tcaacaaacg acaaaatatg aggaactatt
     6361 ttcccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct cttttctaac
     6421 ttctcaatcc tcttccttta ttgatcaaat accaagtaac ggcgtacaaa acaacgatga
     6481 atagcacaag tacgatcatc gatgttgcta ctggcacatc gattgttcca aggaatccgt
     6541 aacggaaacc ggagatcata taaacgatcg gattgacttt cgaaacagct tgccaaaatg
     6601 gcggcaacat agagatggca tagaaaacgc cacctagata agttaacggc tgcaagacaa
     6661 atgtaggcac gatcgataca tcgtcataag actgagcaaa aatcccgttg agcagtcctg
     6721 ctaacgaaaa caagattgcc gtcatcaata atgtaatgat gaccatgaac catgaataca
     6781 catgaagtgg tacaaagaac aaggaaatga tcgttacgag agagccgacg agaatactgc
     6841 gacccactcc gccaatcaca aagccccaaa tgatcacatg cgtaggaaca ggcgctacca
     6901 agatttcttc gatgtttttc tggaattttt gagagaagaa ggacgaagaa acgttggcat
     6961 acgaactagt gatagcagac atcataatca aaccaggcac gatgaattcc atatatgaaa
     7021 aaccacccat gtcaccgatc cgaccaccga tcatttttcc gaaaataaca aagtacaagg
     7081 atgtcgtgat cacaggcgga accagtgttt ggacccagat acgcaaatag cggttcgttt
     7141 ccttgatagc taaacttttt aatgcagtaa aataaagatt aaacattttt ctcctccgta
     7201 tgattatttt cttctgtgat tttcaaaaac agttcttcta aacgattcga cttattcctc
     7261 atagaaagaa ctttgattcc ttgtcttgtc agctgatcga agattccgtt caccccttga
     7321 tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa atgtacaagc gtatccttca
     7381 atcacaggtt tggtctcata tggcgctaaa tcaaagataa atgtttcaaa ttgcagtttg
     7441 gatagaagct ctttcatact agtattctcg atcagttcac ctgattggat gatcccgatg
     7501 ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg tcaaaatgat cgttgtacca
     7561 ttttcgttca attctcggag aaactcccac atctcgcgtc tcaattcaat atcgactcct
     7621 gctgttggtt catccaagat cagcaaacgt ggttcatgca tcaatgccct agcaatcatc
     7681 agtcttcgct tcattccccc ggaaagcata cgtgcacgga catttctctt ttcccaaaga
     7741 ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt ctttgcgaga gacaccgtag
     7801 tagcctgctt gattcacgac gatttgctgg accgtttcaa agggattgaa attgaactct
     7861 tgcgggacta atccaatctg ttgctttgct cggactagat ccgtatccag atcataaccg
     7921 aagacattca cttttccaga tgttttattg actaaagaag taatgattcc gatcgttgta
     7981 gacttcccag ctccatttgg tcctaatagc gcatagaaat ctccttcttt cacggaaaga
     8041 tcgattcctc gcaatgcttc gaccccactt ccataaacct ttttcaaatt atttatttca
     8101 agcgcatttg ccatttattt cctatctcct tatagacatg attctttatt gtatcatgga
     8161 atacagcaga tgaaaaaaca acttggttcc agcgaaaaat caggttgctg ggctcaggaa
     8221 aatctcctaa tgaaagacat caatcggacg aagtcagaca accttttcag aaaaaacaaa
     8281 tcgaagataa aggattacta ttcatatgtg caacaaaagg agcgtgagac aaaagtaaag
     8341 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcaaaagc caagattttt
     8401 gatccaataa atactttcga tcacttcgcc tatcccaata catgccactg ccgcttttca
     8461 tcaaataacg cggcttccgt ttttgcggtt tctactaaag tctctgggta atccaaaact
     8521 ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt cttttaattt gaaatcaaaa
     8581 ctgattcctt ttccttctgt tacttgttct tcaaagtgga cattctcaca ataattcttt
     8641 aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg atactccgca
     8701 agccaccgaa cgactgaagc ggaagaagca atccgttcga tcgtatttgt cccttttaaa
     8761 atctcatcta cgaagcagta gcagcgttct ttggaagcca cttggtccaa caaacgtttg
     8821 atcgatttga tttctgaaac aaaataactg tcgccttcaa ataaatcatc ttcgattgcc
     8881 atggaagtca acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc tgtttggatc
     8941 gtctgtgcta gaatacagct gatggctata cttttgacat acgtagattt tcctgatgca
     9001 ttggaacccg tgactaatgt attcttctgc cagttgaccg gattcactac cgcttctttt
     9061 aacaatggat gatagatatc tttcgctgtc acaccgccct cagtaaactc tggtcggcaa
     9121 gtgatgggca tataagtacg gtaattcaat attgcagcgg ctacttctaa ttctcccagc
     9181 aattcccaca gcttgatggc ttcagttgaa tgcttttcta atttagtgag aacgaaatga
     9241 taggagataa atggcagcat gaacatgatg ttgacatatt caaaaatcaa ctctgcttca
     9301 ctcccactct tcatacgaaa tgaaaaacct aatttaggga tgtctcttaa tggtttagtt
     9361 tgctgtttca gctcttcttg aagtggtgtc ggcagttttg ctagctgatt ggcggctgaa
     9421 atcgttgaaa ccaaataact catgctattc agctctgttt ctagtgtttg tttcttcact
     9481 agataataga ccgtattgaa tactaaccca ccgattgcca aatagattcc tagtacttgt
     9541 cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc caagaaaaat aaataaacct
     9601 acgcgtccaa tctttttact ttttccatcc gctagatatt gttttgcaaa attatgatct
     9661 ttttttccta aacgagcgaa ttgatattgg attttttctc tcagttgagg attttcctga
     9721 taaaaagcaa tcagtttttc taactgctga tcttcaccaa aatcaaagct gcgtaaacgt
     9781 tgatacaacg cttctgaacc tacactagaa taggtactgt tgattccttc gaatacttcg
     9841 aacatatcca ggtcatacca agtaagatca tcgatttcgc tatcccatga acggaacttc
     9901 ttctccgtga gccatgcttc cttcaagctt tcttctttat caaatctcgg ttgataagga
     9961 attttttccc atttgctacg aactgtttcc tttaatttca aacgtgaaaa aatgtctaaa
    10021 gcaactaata aaataacagc tgcaataata attaaaacga taaattctct ttgacccata
    10081 tccctctccg ttcctttatt tgttagcttt agtatagcac tgttgcgcta tttgaaaaga
    10141 gggtaaaaaa acacctttca aaataaaagg tgtttttttc ttgtatatta catatttatc
    10201 tctcatttat caaaattgac aaataatcat agggaggaat tgttgttaat tcttgtttat
    10261 tctccagtgc attgacaact gtcccaaggg aaaggatagc ctcaggtact attttttctt
    10321 gttctgttgg attagctaag cgaagaacaa atccttgttc atgaaaataa gaaggataaa
    10381 tcgatgatac aattaagtct tcaggtaatt caatcaatga aaatttacga ggaacatcaa
    10441 gttcattttg taatggccaa atcttgttat ctaacctgtg aataaataca ttcagtttct
    10501 gcttttgata agaaatagat tgttctaacc gtttttctgc ctgttttgcc actttgaact
    10561 cattaaatct tccttcttct acattaaatc caaaagtgac tttatactca cctatctctt
    10621 gagctagcgg tgtttccatc atgacatgcc cttcatttgt cgtatctcct gaagctcgcc
    10681 caggtcgcca agcaagatta ggttttccaa gttcccctgt tgttgaaaat aaggttatga
    10741 ttaattgatt ttctatacgt tcatactctt ttaaaccatc tgcataaaac gtaaaataat
    10801 gatcattttc tgccacagag acactcttat cgaatatttc taaattaaca ggtttctcta
    10861 ccatagtttc tgaccaattc ggaggaatga catcaggggt attttcaata aatccgtttt
    10921 gaatttgtgc aattgatttt tcagatttat ctaaaacatt aattgctaag cgcatacgat
    10981 gcgagtaaac agtattatga attttgagtg tgccttcaac gatttgactt tctcccagac
    11041 tgaacgatat ttcataagat atttcttcca tttcgggttt ttcagataaa cgatctttta
    11101 aatctttagg aagttgagct tttccgtaaa caactaatgt ttcttgtaaa gaatctttat
    11161 atactttagc tgtttctaat tttagagata gttctgtatc tccttctaat ggtgaataat
    11221 catacgtatc gccatcattt gctgaatcga tcaaatgaac gaaatcaaga tattgtctat
    11281 ctccaactat caaattcaat tgcccatctt taaaagaaag tttatattta ttgttactta
    11341 tctcagtttt gtctatctcg attgctgttt ctaatttttc attatttttc tcaaattcaa
    11401 caacagtgta tcctagtgct ggcacattta catttatttt tatgtcaagt tcataataag
    11461 ctggttcatc aaaatattcg aaacctgtag caatcatccg ataagcattt ttccgttctg
    11521 gaaaataaac ttctttttca atgatattat agggactacc tttgaagtgt attttctttg
    11581 aatcagctac tacatgcacg attttcgatc cactataatt ctcagtcgtt gtgttgaaaa
    11641 caaggacttc attcgatttt agctttaatg catcagccat ccgttttaca ataaggtttt
    11701 cgattccgtc agctatctca ttagcttctt tgattcgatg gaaaatatct tccgcaacat
    11761 tatctgaaac acagccacca atactatcat gagcctggtt ttgcaaaatc tttttccata
    11821 accgacgaag taggccttta ctgatatgga taccatttct ttctgcaatc agcatgagcg
    11881 gctcaactcg ttttagaatt ttttgttcca atttaaaatt ttctattttc atttttgttc
    11941 ttactgagcc aatagaacga tgaacacgag agtaaaccgg ctgacgaagt tcacctttat
    12001 attcaggtaa ttcttttttg tttctgataa cttctacata agaaggataa tctgaaataa
    12061 tattgtggaa tttactgtat ttattcaact tctctacgac ttgatcaaaa tttaaaatca
    12121 ttgacttttg atctacaccc gaaggtacta aaatatcttt gttttttcct tgcatatgaa
    12181 gcatttcaac tgcttcatcc aacttttctt ttgcaaattc aggaatatct ttaattgcgt
    12241 ctaaggtcat cattccagtc atataaccaa atggaaaatt catggcataa acaaatttat
    12301 ctcccaatcc tttccagata aaataaggag atggaaccat cgtttcaaag tttaatcctc
    12361 gccaacacat gaaattatcg atttctactt gatgcaaaag tgtaggcaaa ttcgcattga
    12421 acccaaacgt gtcaggcaaa taccctacca tcattggatc tccatattta aggatcgtat
    12481 cactgattcc aatcattaag tttcttaaaa ttgattcagc atccactaat aaggcatctg
    12541 tttgtgcata ccacggtccc acgaatagtt gtcctgtttt catcagtcgt tggataacct
    12601 taagcttttg cggattgatc tccaaatact catcgacgat tgaagtttgt ccatctaaac
    12661 aaaagtttac tttagggttc ctttccaatt catctagaac ttcagtaaag acctgatcag
    12721 acaatacgat ggcatcttca tttgtaaaat accattctct gtcccagtgc gtgtgttgaa
    12781 ttatatgtgc atttaccata tcagattctc ctactttcct tcttctactg aatttacttg
    12841 ttgttctttt ttatacttga tggattttag tgcattgacc attaacgctg taaccactcc
    12901 cccaataaca attgatacta aatataaagg tatattcgta acaaaaggaa ttgccataaa
    12961 ggttgaaagc attgtttctt gagtaacacc aaaaaatcca tttagaccac atgctactgc
    13021 agtgcccacc acgatgcttg gtattactcg taaaggatct ttcacggcaa acgggatcgt
    13081 cccttcagta atccccacaa ggcccataat catagctgtc tttgcattat ctcgttccga
    13141 ttcatcataa agttttttgt ttaaaattgt tgctaaaaac attccaaatg gcggaatagc
    13201 aattcctaat aacatggctg tactaggtcc aaagcttccg cttgaaaaag aagcaacccc
    13261 aaaagcatat gcgattttat tgataggtcc tcccatatca aacgccatca ttcctcctaa
    13321 aatagccgac atcaaagcag catttgctcc tgacatgcct tctagccaat tagtcagtcc
    13381 tgttgtcatt gcttgaatag gcgtagctag tacataccat aatatgattc caacaactaa
    13441 tgtagtaaca aaaggaatca gcataattga ttttagacta ataacttgca tcggtacttt
    13501 tacttttttc aatgcattag ctaaatagcc acaagctaat ccagcaacca ttcctccaat
    13561 aaatcccata ccaagatttt gacacatcat cccagtgata aaagctggtg ctagaccggg
    13621 acggtcagca attgaatatg cgatataggc tgaaatcatc ggtacgataa actgtaatcc
    13681 cgtctgacca atcattcgca ggactcccca gaatgtatcc gtctggtccc aaacacctgt
    13741 gccaccaaaa atgactgcaa tagaagttag taaccctgct gctacgacaa gcgggatcat
    13801 ataggaaacc ccactttgga aatgatttag aatttctctt ccttgtttct tgaattccat
    13861 tagtaactcc cccacgatat attttttatt tgttaattaa tgcaattgct tcatcaatcg
    13921 tctcttcacc atcttttact acctctgagg tccctactgt taaaatcggt ttaccgtcaa
    13981 agcgacctcg ttctgaaaca tttgtatcta ctgcaaatat gacaacatct gcctcggaaa
    14041 catccgaaat tgaaagtttg ttttctatcc ccatagcccc ctgtgtttct actttaatat
    14101 ctaacccttt cttcttcgca aacttttcta aattagcttt tgccatataa gtatgggcaa
    14161 tccctgcgat acatgctgtt actgctacta tttttgtcat atgaaattcc tccttttaaa
    14221 atgatagagc ttttaccaat tcttttttat ttctcgaatc ttttattctt ttaacaaact
    14281 cgtcatccat caatcttcca gataattctg ctaaaatacg taaatgatta gtacctttat
    14341 cagtgtcttt tattgctaac aaaaagacga gatcaactgg ttgatcatct aatgcgcccc
    14401 attcaacaga ccgctttaac ttggctacag ccactgttga ttcaattact gctaggcttt
    14461 tgccatgagg aatggcgata tttttcccaa ttcctgtggt catatggttt tctctgtcaa
    14521 atacgtcttt ttgaaattgt ttgtggtcag acacaaaccc attagtctct aacaatttcg
    14581 ataatttttc gactacactt tttttatcag atgcatttaa atctatcact atattattta
    14641 tattgatttt ttcactgatc atttataagc cttctttcaa tatataaact aatttttcaa
    14701 acgacgaact cgtttttaaa tttgctaata actcatgatt cgaagcgatt tcattgaatg
    14761 tttcataaat tttattcaat tcaggtttta ctttttcatt aagtgcaaaa aagaaaacag
    14821 aatgaacaag tgtcccatcc caatcaattc cttctggatt aacgtagaca cagattactg
    14881 gcttttttat aaaatctact tcgatatgcg gcatagcaaa atcattcatc gctgttgaag
    14941 atagtttttc tcttttgatt gctccctcta gtatcccttc tttcgcgtaa ttttctttta
    15001 ccaatttttt ggtaattata tttaaaacgt tcacttgatt atcctttatt tggtgggtaa
    15061 aaaagcaaaa tcgtttatct aataaattta gaaaagcatc ttctgtttgt tcatttaact
    15121 gtagatactg ttttactttt ctgacatctg cccctaacat caaaggacta acaacaataa
    15181 caggaaaatt tgtattttca atcggaatcg tgcttataac aagttcatca gtgacttttc
    15241 tatcttgtag ttcattgatg cttagtacat cttctatctc aaccatatct gcaaaaccgt
    15301 tatttattct ttgctctaat aattttgatg ttccatatcc gctactacaa actaatatca
    15361 ctttcgtttt gtcaggtttg taacgatcca aaagtgattg aatatggagt gttacgtaag
    15421 cgatttcatc ttcattaaaa tgtatacaga atctctcttc gatttcctct aataaatcta
    15481 cacttatcat aaatgacctt ttaaaagaat atttgatttt gtccgtataa gggttataga
    15541 tatttactcc caatttgagt cttttaactg ctgcattgag atgtaatact agattattga
    15601 tcaattcttc atcaggataa aattcattta atttaattct tactaactct tgtagctcaa
    15661 cacccatatc taaataggaa agcttcgttt gatctaagtc gtgattcata gcattttttt
    15721 caatcgaagt aatgtgaatg tcaacataag cttgttcatc tattggcaca ataattgaaa
    15781 atgctttttc aatcattttg attaaaataa gtgtattctt tgagatttct tcttcagaat
    15841 tattactacg tgataagaaa ttgcttgttt caatcctttc tatcgcaata gcaaggtgga
    15901 tagctaaagt ttgaaattca taatctgtaa atttcaattg tgtcttattc atgtatcgat
    15961 taagcaattc ggctactttt ttcaatatgt ccttatttaa gaaattattc gcttcaccaa
    16021 tgaaattgat attcaagtct aagagatctg ttttactgtt agatgctgta aagccacccc
    16081 aataatagtt cacaacttct gacatcaacc ttcttttact tttttcatca gctttgatgc
    16141 gcacgccatt tcgatctgaa actaaggaca tcccatgctt gtttagtctt tttctaacat
    16201 ctttcattgt gctttctacc gtcgacctgc tcacgtataa ttcgtcagca agattttgaa
    16261 tctttagaga atcattagta ttcaataatt tagcaaacac ataagttaga cggtcctccg
    16321 gagagtctgc gatattgatt ttatgactgg ataataaatt tcgaagtgtt tctttttttc
    16381 caataagcga aacgcctttt ttaggttgaa ccaacatttg tacatctggc attttttgga
    16441 ttatttcttt caaaattaaa atatctttgc taattgtttt cgtagataca tataattgtt
    16501 ctgataattc tttgtaagtt gtgtgctcat tttcaatcaa aaagtatagt attttcattt
    16561 gccgttcgct aaattcgatg cccattatct tcacctcctg aatacagctt aagtgtacaa
    16621 ttattatctc tattttcata cagaagaaag ttcttgatta atgtagaagt ttctaatatt
    16681 cttgattatt tcaaatcaat aaaaaagcct gcaatattta tatca
//
LOCUS       DAYYAG010000059        16714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000059 DAYYAG010000000
VERSION     DAYYAG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.059"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACHARQ_001641"
     CDS             complement(251..1462)
                     /locus_tag="ACHARQ_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8410050.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACHARQ_001642"
     CDS             complement(1581..2216)
                     /locus_tag="ACHARQ_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8410051.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACHARQ_001643"
     CDS             complement(2352..3005)
                     /locus_tag="ACHARQ_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410052.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACHARQ_001644"
     CDS             complement(3108..3314)
                     /locus_tag="ACHARQ_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM8410053.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACHARQ_001645"
     CDS             complement(3390..4136)
                     /locus_tag="ACHARQ_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM8410054.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACHARQ_001646"
     CDS             4296..4520
                     /locus_tag="ACHARQ_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM8410055.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACHARQ_001647"
     CDS             4544..5305
                     /locus_tag="ACHARQ_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8410056.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACHARQ_001648"
     CDS             5317..5574
                     /locus_tag="ACHARQ_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410057.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACHARQ_001649"
     CDS             5666..5917
                     /locus_tag="ACHARQ_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8410058.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5893..6066
                     /locus_tag="ACHARQ_001650"
     CDS             5893..6066
                     /locus_tag="ACHARQ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410059.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6180..6587)
                     /locus_tag="ACHARQ_001651"
     CDS             complement(6180..6587)
                     /locus_tag="ACHARQ_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410060.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACHARQ_001652"
     CDS             6748..6972
                     /locus_tag="ACHARQ_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410061.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACHARQ_001653"
     CDS             6982..7176
                     /locus_tag="ACHARQ_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410062.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACHARQ_001654"
     CDS             7179..8201
                     /locus_tag="ACHARQ_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8410063.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACHARQ_001655"
     CDS             8164..8979
                     /locus_tag="ACHARQ_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM8410064.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACHARQ_001656"
     CDS             8997..9779
                     /locus_tag="ACHARQ_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410065.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACHARQ_001657"
     CDS             9779..10627
                     /locus_tag="ACHARQ_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8410066.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACHARQ_001658"
     CDS             10624..10866
                     /locus_tag="ACHARQ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410067.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACHARQ_001659"
     CDS             11009..11377
                     /locus_tag="ACHARQ_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8410068.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACHARQ_001660"
     CDS             11392..11553
                     /locus_tag="ACHARQ_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8410069.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACHARQ_001661"
     CDS             11550..11876
                     /locus_tag="ACHARQ_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410070.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACHARQ_001662"
     CDS             11873..12688
                     /locus_tag="ACHARQ_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM8410071.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12690..12815
                     /locus_tag="ACHARQ_001663"
     CDS             12690..12815
                     /locus_tag="ACHARQ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410072.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12827..13138
                     /locus_tag="ACHARQ_001664"
     CDS             12827..13138
                     /locus_tag="ACHARQ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8410073.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACHARQ_001665"
     CDS             13135..13380
                     /locus_tag="ACHARQ_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410074.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACHARQ_001666"
     CDS             13377..13793
                     /locus_tag="ACHARQ_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8410075.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACHARQ_001667"
     CDS             13790..14128
                     /locus_tag="ACHARQ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410076.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACHARQ_001668"
     CDS             14125..14355
                     /locus_tag="ACHARQ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410077.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACHARQ_001669"
     CDS             14362..14565
                     /locus_tag="ACHARQ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8410078.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACHARQ_001670"
     CDS             14562..14858
                     /locus_tag="ACHARQ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410079.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACHARQ_001671"
     CDS             14935..15348
                     /locus_tag="ACHARQ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8410080.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACHARQ_001672"
     CDS             15361..15747
                     /locus_tag="ACHARQ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410081.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACHARQ_001673"
     CDS             16298..16525
                     /locus_tag="ACHARQ_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410082.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="ACHARQ_001674"
     CDS             16522..>16714
                     /locus_tag="ACHARQ_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410083.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DAYYAG010000060        16582 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000060 DAYYAG010000000
VERSION     DAYYAG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.060"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACHARQ_001675"
     CDS             <1..546
                     /locus_tag="ACHARQ_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8410084.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="ACHARQ_001676"
     CDS             735..1403
                     /locus_tag="ACHARQ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8410085.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1923)
                     /locus_tag="ACHARQ_001677"
     CDS             complement(1603..1923)
                     /locus_tag="ACHARQ_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410086.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1960..3342)
                     /locus_tag="ACHARQ_001678"
     CDS             complement(1960..3342)
                     /locus_tag="ACHARQ_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8410087.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="ACHARQ_001679"
     CDS             complement(3498..4685)
                     /locus_tag="ACHARQ_001679"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8410088.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="ACHARQ_001680"
     CDS             complement(4692..5723)
                     /locus_tag="ACHARQ_001680"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8410089.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5769..7193)
                     /locus_tag="ACHARQ_001681"
     CDS             complement(5769..7193)
                     /locus_tag="ACHARQ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8410090.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7368..8273
                     /locus_tag="ACHARQ_001682"
     CDS             7368..8273
                     /locus_tag="ACHARQ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8410091.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="ACHARQ_001683"
     CDS             8364..9041
                     /locus_tag="ACHARQ_001683"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8410092.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9038..9208
                     /locus_tag="ACHARQ_001684"
     CDS             9038..9208
                     /locus_tag="ACHARQ_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410093.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9322..10218
                     /locus_tag="ACHARQ_001685"
     CDS             9322..10218
                     /locus_tag="ACHARQ_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8410094.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="ACHARQ_001686"
     CDS             complement(10273..11643)
                     /locus_tag="ACHARQ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8410095.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="ACHARQ_001687"
     CDS             11881..12627
                     /locus_tag="ACHARQ_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8410096.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="ACHARQ_001688"
     CDS             12624..13463
                     /locus_tag="ACHARQ_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8410097.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14413
                     /locus_tag="ACHARQ_001689"
                     /pseudo
     CDS             13464..14413
                     /locus_tag="ACHARQ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14554..15306
                     /locus_tag="ACHARQ_001690"
     CDS             14554..15306
                     /locus_tag="ACHARQ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410098.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15325..16476
                     /gene="queG"
                     /locus_tag="ACHARQ_001691"
     CDS             15325..16476
                     /gene="queG"
                     /locus_tag="ACHARQ_001691"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8410099.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5394 a         3091 c         3328 g         4769 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgcaaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt aatcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaatgca gaagcatatg tggaaaaact aaaagaatta gatacacaag cgaaggaaag
    14041 ttttgcttct atcgaagaga acaaaaaatt attagtaaca agtgaagggg cattcaagta
    14101 tttttcacga gcatatgatt tgccagcagc ttatatatgg gagatcaata cagaaagtca
    14161 aggtacgcct gatcaaatga aagcgatcat tgatcagata agagcaaaag aagtaccggt
    14221 tttattcgtg gaaaccagtg tagactcaag aagcatggaa cgggtagcaa aagaaacggg
    14281 tttgaaaatc tatgataaac tgttcactga ttccatagca aaagaaggag aacaaggaga
    14341 ttcttactat cagatgatga aatggaatat tgaaacgatc catgaaggtt tgtcacaaac
    14401 aaaagaaagc tgaaaagtgc aaggtctagc ttgaggagca gaccttgcat tttttataag
    14461 agatatgaaa aaaaattatg ctgtttttcg tcgtacagat accttttttg aagaaattcc
    14521 gttatagtta gagtaaggaa ggaaggtgtg gctatgaatt ttacacgtaa agataaatat
    14581 acgattaaag aagaagtctt tcgattgatc agccagcagg aaaataccga caaggatcaa
    14641 gaccttcaat ttcttgaagc agaattagct aaattattca tagcaaatca aaatgagaca
    14701 acagaaccag atgaagagct gtacataggc aaatggttgt ttgatgaatt tcagccttta
    14761 gatgaaaata ccggtatcgg ttattttgtg ttcctttctc cagtcggctt cacacctggc
    14821 gtattagtta aacaaaacga cgaaataacc gtcaaagcat ggcctgcatt attttcagtc
    14881 atcaaagcag aaggagtaat caaaaagatc cagtcggcct attataaaag tcaatttatt
    14941 gccaacagca aattaatgaa tatgttttat aatcgtctag tagaaattgc taggcctggg
    15001 aaaatcccgc aaacattcac gtacacacag caaaaaaatc tctatgaaga agtattgtat
    15061 tcccgcgatc tgtctgagtt ttcgccatgg gaagaggtaa ggctccatga actgttaagt
    15121 acaaaaccag tttcattgaa tttttttaat gcattagata acaaaagaaa attaatttca
    15181 acaaattatt gggaagtatt tgctgcaaga aaaaaatgga tggaagaaaa gaagattgcc
    15241 gatcagcaga tccagtttgt ccagctgaaa aaagaaaaac tatattatgg gttatacagc
    15301 cgttaacagc aagcgaggaa gaccatgaca ttaaaagaag aaatcataca agaaagcaaa
    15361 cgattaggaa ttgacaaaat cgggttcacc acggcggaac cttttgattc attaaaagaa
    15421 tcgttggaag aacaaaaagc agctggccat acttctggct ttgaacatcc aaatatcgat
    15481 gagcgtcttt atccagaaat gacatttgat cagccgaaat cgatcatttc cattgcactt
    15541 gcgtatccta ctaggatcca tgaagaagtg cctagggatg aaaaacgcgg gcagtttgct
    15601 cgtgcttctt gggggatcga ttaccatgat atactcagag accgtttgaa ccgattgatc
    15661 gaattcatcc aaacaagagc agaaaaatgg caaaaagaag aggaatggcg tctagctccg
    15721 caagtagata ctggagaact gatcgatgtc gctgtagcac agcgagctgg tttaggattt
    15781 attggacgaa acggattgtt gatcactgaa gagttcggtt catttgtcta tcttggtgag
    15841 atcgttacta atattgtatt tgagcctgat aaaccaggag aatttggttg cggtgactgt
    15901 atgcgctgcg tgactgcttg tccgacaaaa gcattgcttg gtgacggaag aatgaatgcc
    15961 cagcgatgtt tgtcttatca aactcagaca aaagggatga tgccggaaga atatcgtaaa
    16021 aaaatgcgaa atgtcattta tggatgtgat atctgccagt tagtctgccc ttacaatcaa
    16081 ggaaaagatt ttcattttca tgaagagatg gaaccaaaag tagacgaaat atatccaaaa
    16141 ttaaagccaa tgctttcgat gtctaataaa gacttcaaac agcaatttgg gcatttagct
    16201 ggttcatggc gtgggaagaa acctttgcag agaaacgcat tgattgcttt agctaatcta
    16261 ggtgatcgaa cggcattgcc tgagatttca gaatgtttaa cagatgtccg ccctgtcatc
    16321 agaggaacag cagcttgggc tattggacgt ctaggaacaa aagaaccgga aaaatggctt
    16381 gatgtattag atgaaatatt agctaaagaa acagaagaag tcgttataaa cgaattggaa
    16441 catgcccaga aattattgcg gagaaagtta aaataaaaaa atagttaaca ttattcaaag
    16501 tttttgtcca gtaattgttg taacaattac tggttttttt gtatttttta cattttttta
    16561 agagaatttg ataaaaatca aa
//
LOCUS       DAYYAG010000061        16460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000061 DAYYAG010000000
VERSION     DAYYAG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.061"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..1622)
                     /gene="guaB"
                     /locus_tag="ACHARQ_001692"
     CDS             complement(138..1622)
                     /gene="guaB"
                     /locus_tag="ACHARQ_001692"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8410100.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2127..2822)
                     /locus_tag="ACHARQ_001693"
     CDS             complement(2127..2822)
                     /locus_tag="ACHARQ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8410101.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(2912..4012)
                     /gene="ychF"
                     /locus_tag="ACHARQ_001694"
     CDS             complement(2912..4012)
                     /gene="ychF"
                     /locus_tag="ACHARQ_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8410102.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(4021..4212)
                     /locus_tag="ACHARQ_001695"
     CDS             complement(4021..4212)
                     /locus_tag="ACHARQ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8410103.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4233..5123)
                     /locus_tag="ACHARQ_001696"
     CDS             complement(4233..5123)
                     /locus_tag="ACHARQ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8410104.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5110..5877)
                     /locus_tag="ACHARQ_001697"
     CDS             complement(5110..5877)
                     /locus_tag="ACHARQ_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8410105.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(5889..6602)
                     /gene="rsmG"
                     /locus_tag="ACHARQ_001698"
     CDS             complement(5889..6602)
                     /gene="rsmG"
                     /locus_tag="ACHARQ_001698"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8410106.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(6834..8735)
                     /gene="mnmG"
                     /locus_tag="ACHARQ_001699"
     CDS             complement(6834..8735)
                     /gene="mnmG"
                     /locus_tag="ACHARQ_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8410107.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(8752..10149)
                     /gene="mnmE"
                     /locus_tag="ACHARQ_001700"
     CDS             complement(8752..10149)
                     /gene="mnmE"
                     /locus_tag="ACHARQ_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8410108.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10448..10831
                     /locus_tag="ACHARQ_001701"
     CDS             10448..10831
                     /locus_tag="ACHARQ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410109.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(10999..11757)
                     /gene="jag"
                     /locus_tag="ACHARQ_001702"
     CDS             complement(10999..11757)
                     /gene="jag"
                     /locus_tag="ACHARQ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8410110.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(11775..12593)
                     /locus_tag="ACHARQ_001703"
     CDS             complement(11775..12593)
                     /locus_tag="ACHARQ_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8410111.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12590..12946)
                     /gene="rnpA"
                     /locus_tag="ACHARQ_001704"
     CDS             complement(12590..12946)
                     /gene="rnpA"
                     /locus_tag="ACHARQ_001704"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8410112.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13064..13198)
                     /gene="rpmH"
                     /locus_tag="ACHARQ_001705"
     CDS             complement(13064..13198)
                     /gene="rpmH"
                     /locus_tag="ACHARQ_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8410113.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            13721..15055
                     /gene="dnaA"
                     /locus_tag="ACHARQ_001706"
     CDS             13721..15055
                     /gene="dnaA"
                     /locus_tag="ACHARQ_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8410114.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15253..16383
                     /gene="dnaN"
                     /locus_tag="ACHARQ_001707"
     CDS             15253..16383
                     /gene="dnaN"
                     /locus_tag="ACHARQ_001707"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8410115.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4532 a         3491 c         2943 g         5494 t
ORIGIN      
        1 ttcacgaaaa ttgcttttca aatttttgtg aatttcttct tatttccgaa ggagttgctt
       61 ctacttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc
      121 cacgctcttt aatgttttta agattcaact gaatagtttg gtgcttcttt tgtgatttgt
      181 acatcatgag ggtgtgattc ttttaatcca ctgccgctca tttggataaa ttgcgcttca
      241 tcacgtaatt gctgtagatt ggctgcccca acataaccca ttcctgaacg caaaccgccg
      301 atcatttgga aaataatatc tgcaacactt cctttataag caacccgacc ttcgattcct
      361 tctggtacta atttatttgc ttcattgaca ctaccttgga agtaacggtc ttttgaacct
      421 ttttccatcg cgcctaatga gcccatacct cggtatgttt tgaatcgacg tccttggaag
      481 atttcgaatt cacctggtga ttcatctgtc cctgccaaca tacttcctag catcactgca
      541 tgtccgccgg cagctaatgc tttgacgatg tcaccagaat atttgatccc tccgtctgca
      601 ataatcgctt tgccatattg acgagcaact gatgctgcat cataaatagc tgtgagctga
      661 ggtacaccta caccggctac cacacgtgtc gtacaaatcg atcctggtcc gataccaact
      721 tttacgacat ctacacctgc atcatataaa gcttttgtcg cttcagccgt cgcaacgttt
      781 cctgcaatca atgttgcatc tgcaaaagtt gaacggattt cttgaatttt tcggatcaca
      841 cctgcactat gtccatgagc tgtatcgatg atgattgcat ctgcccctgc ttctaataaa
      901 gcattcgcac gctcaaatgt atcgcttgtt acaccaacgg ctgctgctac aagcaatcga
      961 ccatgttcat cttttgctgc atttggaaat tcgatcactt tctcgatgtc tttgatcgtg
     1021 atcaatccgc ttaaacgacc ttcgttatct acgattggta atttttcgat tttatgtttt
     1081 tgcaaaatct tttctgcatc ttttagagag gtacctactg gggcagtcac taagttgtct
     1141 tttgtcatca catcagcaat cggcatgtga taatctgtta caaaacgcat gtcgcggttt
     1201 gtaattatcc ctaccaattt tctgttttca agtgtttcaa cgattggcac accactgata
     1261 cgatatttgc tcatcaattc ttctgcgtct gctacaagat gctgtggcgt taagaagaac
     1321 ggatcaatga ttacgccact ttcagaacgt ttgacttttc ttacttcatc cgcttgctgg
     1381 ctgatcgtca tgtttttatg gatcacacct agtccgcctt gtcttgccat agcaatggcc
     1441 atcttgctgt cggtaacagt atccatactt gcactgatga ttggaatatt caacttaata
     1501 tttttagcta attgtacgct catatccact tcgtttggta acacgtgact ttctgctggt
     1561 atcaataaga catcatcaaa agtaaatcct tttttcgtga actttgtttc ccagttggac
     1621 atttttagta ccactcctct aatttagttt atctattaaa ataattgatt ttttcattta
     1681 agtcaatgat ctacttggaa tcaagttagc tttctcatag aataccaata taaagcgttt
     1741 acatctgtgt gtattaaaag agattgtaaa aaagctgtcc tgcatcaagt aataagaaca
     1801 gccaatcaaa aacagcttct catgtttttg aattggttgg gtttattacc gcagatgcaa
     1861 acttttgaac accgtttagt ggagaggctg tgaaaaagct gcttagtttc gagtagtaag
     1921 aaaaattttg tgaaaatggc tttccacatt tttgcataaa ttggacttaa taccgagaaa
     1981 ctggcttttg aacaccgttt atcgtaaaaa gggactgtga caagactttt gtcacagtcc
     2041 cttactatta tttaactatg gataaacaaa atgaggttct ttactacccc tagatatcta
     2101 gcaaaatagc tgatcttttt gctagtttat cttcttggga cggataaact tccaataatc
     2161 ccgtattttt tcttcaagta gtaacggatt gctaaagcag ttgccccgat aatgataact
     2221 actactggat ctaatactgg attgataact ggtggcaata gtgttgtcgc agaaaacagt
     2281 acgacccaaa gaagtgtcgt tccacctagg ataagcaatg tcttacccat tcctggtttc
     2341 ttgcttcgat ctgctccggg atattcatat tgatagatgt atttgtacat catataaaag
     2401 acccagccac cgattgcaga acctagaacg agtgtagaaa agccataagc tggcgttgtt
     2461 ccacgtgaaa acaatccggt aatcgcaatc atgatactta atagaccaag caacagcaac
     2521 gtattatcta accacatcaa taaaggggtg gatgaatttt ttacttctgc tggcttattg
     2581 atgattgctt ccgttctttc agagactgtc ccaaacaatt gacgggctgt tcttccgcct
     2641 ttttgttctg caactaaagt aggcagaatg tcatgaaggg cttttacttt ttcttcttca
     2701 gacaaattcg ctgcttctaa agattttttc aaatcaaaaa tgtactgttg atttcttttg
     2761 gttaattttg tttctaaact attgttttct tttacgattg ctcgtaattg ttctggttcc
     2821 attatctaat agtcctcctt agccgttcct tttctcaacg gtcgtcacaa aagacattct
     2881 accatattct acagctagtt tgatagccct tttacacatt aaagcggaat aacattacgt
     2941 cgccgtcttg tacgacataa tcttttcctt ctaatcgaac acgtccagct tcttttgctg
     3001 catgcatgtt tccgtaatga tccaagtctt caaaagagac agtttctgca cggataaatc
     3061 cgcgttcgaa atctgaatgg atgattcctg ctgcttgtgg tgctttgatt ccttttttga
     3121 atgtccaagc acgtacttct tgttccccag cagtaaagta agtagctaag cctaacaaat
     3181 cataagcagc acggatcaac tgatccaaac ctgattcttc gattcctaaa gcttctaaaa
     3241 attccgcttt gtcttcttca tctaattctg cgatttcttc ttctgcacga gcactgacca
     3301 cgatcacttc tgcattttct tctgcagcaa agttgcgcac ttcttgtaca taatgattcg
     3361 cgtctgcatc tgctacgtca tcttctgaga cattggctac ataaagaatt ggttttgtcg
     3421 tcaataaaaa tagtgattta acgatttttt gttcttcttc agtaaattcg atcgttcggg
     3481 cagattttcc ttcttccagt acaggtttga ttttatccag tacagctagt tctgccactg
     3541 catctttatc tttggtttta gcaacttttg ctacacgagt atagcgtttg ttgatagatt
     3601 ctaagtctgc taacactagt tccaagttga tcgtatcaat atcttctagt ggatcaacac
     3661 gtccttctac gtgagtaata ttatcatcat caaaacagcg aacaacgtga caaatagcgt
     3721 ctacttggcg gatatggctt aagaattgat ttcccaaacc ttctccttta cttgcgcctt
     3781 ttacgattcc agcaatatct gtaaattcaa aagtcgtcgg aacagttttt ttcggatgta
     3841 caagttctgt taagcgttgc aaacgccagt caggtacttc taccatccct acgttggggt
     3901 cgatcgtcgc aaagggatag tttgctgctt ctgctcctgc ttttgtgatc gcattaaaca
     3961 aagttgattt tcctacgttg ggcaacccaa caattccagc agtcaatgcc ataaatttat
     4021 tcactctttc tctgcatttt tttcaattac tttttttaat ttcttttcaa aatctcgacg
     4081 ggtcatcatg acgatatgtc cgcaattcat acatctgatt ttgatatctg cgcccattcg
     4141 aatgacttcc cagcgattcg tctggcaagc gtgaggtttt ttcatttcaa cgatatcgtg
     4201 caagtcgtac ataaaaatag cctccttttt gtttattctt catcaaattg aatattcaag
     4261 atatcaagaa ttcgagtcaa atctttttga gaaagatatt caatctcgat cttacctttc
     4321 ccattttttt cttggatagc tacacttgta ccaaatttat ccatcaaacg atcttcgctt
     4381 tctcggatat aatatggttt ttcctttgct agccttggga tttttttctt ttcttttcca
     4441 tcattttgat ttaactcaga aacaagctgt tccagttggc ggacagttaa attttcagtt
     4501 actgcacgat tggctaattt caagatcaat gttttatttt ttaaacctaa cagcgtacgg
     4561 gcttgtccca tagataaacg ctgatcttgt accatttcct tgacaagatc cggtaaagaa
     4621 agcaagcgca aataattggc gatataaggt ctgctctttc ctaaacgact agctacttct
     4681 gcttgtgtta gtttcaggtt tttcatcagc atctcatatg cttctgcttc ttctaacgga
     4741 ttcagatctt ctcgctgcaa gttttccagc actgctactt gcatcatcgc ttcttcgtca
     4801 aagtctctaa cgatagcagg gatcgtttct ttttcagcta gtttggacgc tcgaaaacgt
     4861 cgctcccctg caataatctc atatcctttt actgcggaac ggcgtacgat gatgggttga
     4921 aagacacctg attgttcaat agaattagct agttcctgca aagatacttc ttcaaatgtt
     4981 ttacggggct gatacggatt tggtcgcagc tcattcaatg gtagctggac aacggtatca
     5041 tcttttacat ccaccgtctc taagttttct aaatcttgaa acagtgcatc gattcctcgg
     5101 cctaatcctt tacttttctt cacgacttac cacttccttt gccaatgctt ggtagacttc
     5161 agctcctcga gaacgtggat cgtagtcgat gatcggtttt ccatgactag gtgcttctga
     5221 taaacgaata tttcttggaa tgatcgtttc gtaaactttt tcgcggaagt atttacgtac
     5281 ttcttctacg acttcatttc ccagatttgt tcgtgcatca tacattgtca gtaagactcc
     5341 ctcgatctct aattctggat taaagtgttt ttggactaaa cgaaccgtat tcagtaattg
     5401 gcttaatcct tccaatgcgt aatactcaca ctgaacaggt atcaaaatcg aatcacttgc
     5461 tgtaaatgaa ttgattgtca gatggccaag agaaggcggg caatcgatca aaatataatc
     5521 atactgactg cttacttctg ctaaagatcc ttttaatctt gattctcttg ccatcattga
     5581 cgtcaattcg atctctgctc ctgctaattg gagagtcgca ggcacaatac tcaaattttc
     5641 gtgttcagtg atcaaagttg cctcatcaat tggcaattca ttgactagaa catcatagat
     5701 gtctcttgtc acatcaggtt ttctgatccc gacaccactg gtagcatttc cttgtgcatc
     5761 catatccact agcaatactt ttttgccaag gctagcaaga caagcaccta gattcaccgt
     5821 cgtcgttgtt ttgccgacgc cacctttttg gttcgcaaca gatattattt gtgccatctc
     5881 ttctcctcct atttgattgg ctgcttatta ggcagtccgg gctttcttgg gtatttcttt
     5941 ggggttggtt tctttttttg aataacgatg atatgccgtt gatcagagac attcggcaag
     6001 gtaaccgcct cttctttgag gaatttacca cctaataaag caatcgcttg ttttgcttct
     6061 gtcaattcct cttcactttt tgctgctttc aatgcataaa aatagccttc ttgtttagcc
     6121 aagggcaaac acaattccgc taacacattc aaacgagcaa ccgcacgagc agtgacaagg
     6181 tcaaatgatg cacgaaagtg aggattttgc ccaaatgttt ctgcccggtc atgatataag
     6241 gaagcatcga ttcctaataa tcgggttaac tcagataaga aggttattcg tttattcaga
     6301 gaatcaacga tcgttatctg cagttctgga aaggcaattt ttaaaggtat gctaggaaac
     6361 cctgcccccg aaccaacatc gcataaagat aaaggttgcg taaagtctac ttcaaatgct
     6421 aatgtaagtg aatcgtaaaa atgcttcaga tatacgtctt tttcatccgt aatagctgta
     6481 aggttcatct tttcgttcca ttcgactaat gtccgataat aaatagcgaa ttgattcatt
     6541 tgatgatcgg taagctctat ccctttttct gccaaggatt ggcgaaattc ttctggtatc
     6601 ataaatattc ctttcattgt gaaacagcaa ttgtttcaca gattctctcc ctactttaac
     6661 gcaaaatctc tagacatgca atccttccat atggattctt aaaaatttga aaagtaagaa
     6721 aagccgatag actttttctt tctagtatga ctcataaaat atacacagaa gagagaacta
     6781 gctttgagca atttctgcaa aaactagttc tctcttctat ttattattac gtattacgct
     6841 ggttcactga ctttcgcaat cttcccttgt tcgatataga ccattaggat acttaggtct
     6901 gcagggttta cgccactgat tcggctagct tgggcaattg tttctggttg gattttctgc
     6961 aatttttgtt ttgcttctgt tgctaatccg ttgattgctg cgtagtcgat gttttctgga
     7021 atctttttcg cttccatgcg ttttaatttt tctacttttt ccatggcttt tttgatgtag
     7081 ccttcatatt tgatttggat ttctacttgt tcgatttctt ttcgtgtcaa ttcttcattt
     7141 tctggaataa accgtagaag ttctgcataa gtaatctcag gacgtcttaa aaagtcgctt
     7201 gctagtacac catcttttaa tggtgccgca ttaatcgttg ctaaaaagtc ttggacttct
     7261 tctgttggtt tgattcgtac attcatcaaa cgtttgattt cttgttcaac tgcttctttt
     7321 ttcttcaaat aattcttgta ttgatcttct tttactaagc ctacacggtg acccatttct
     7381 gttaatcgca aatctgcatt atcatgacgt aaaagcagac ggtattcagc acgtgaagtc
     7441 agcaaacgat aaggttcgtt cgttcctttt gttaccaaat catcgatcat gacaccgatg
     7501 taaccgtcac ttcgtttgat cactaaaggt tcttttccct gaactttcaa agctgcattg
     7561 ataccggcaa tcagcccttg tccagccgct tcttcataac cgcttgtacc atttgtttgt
     7621 ccagctgtat aaagattttc aacgacttta gtttctaatg tcggacggag ctggtgtggc
     7681 tctactacgt catattcgat agcatatcct gtacgcatca tttctacttt ttctaatcca
     7741 gcaatagaat gaagcatgtt gacttgaaca tcctctggta aagaagtaga taaaccttga
     7801 acatagactt cttccgtatt taagccttct ggttccaaaa ataattgatg acgtggtttg
     7861 tctgcaaaac gcacgatctt atcttcgatg gatggacaat agcgagcgcc taccccttca
     7921 acgatccctg taaacattgg ggcacgatgt aagttgtctc gaataatttg gtgagtcgtc
     7981 tcacctgtat aggtcagcca gcaagattcc tgatctagtt tataggcact atctggtgtg
     8041 ctgaagctaa aatgattcgg ctctttgtca ccaggctgct cttctgtaac agaatagtcg
     8101 atcgtacttg attttactcg aggcggtgtt cctgttttga accgttcgat agcaaagcca
     8161 agttctttta gatgattcgc tagtccaata gaaggttgag agttattcgg acctgatgaa
     8221 tatttcaatt caccgataat gatttctccg cgaagtgctg ttccggctgt aataacaact
     8281 gctttggccc ggtagctcgc tcctgtggac gtcactactc catgacaaac accatcttcg
     8341 acgatcagtt cttcgacgat tccttgtctt agcgtcaaat ttggttcttt ttcgatcgta
     8401 tgtttcattt ctgttgaata agcatgtttg tctgcttgtg ctcgcaatgc tcggacagct
     8461 gggccttttc ctgtgttcaa catacgcatt tggatatagg ttttatcgat attcttaccc
     8521 atttctccgc ctaacgcatc gatttctcga acgacaacgc ctttagctgg tccgcctaca
     8581 gaaggattac atggcatgaa tgctaccatg tccaaattta ttgtcagtaa taatgttttt
     8641 gatcccatac gtgcagcagc aagtgcagct tctgaaccgg cgtgtcctgc gccaacaacg
     8701 atcacgtcat acgcatctgc ttcataatgt tccaatcgtt tgttcctcct tttattttcc
     8761 taaacaaaat tgactgaata attgagtgat tagctcatct tgtacactgt cgccgacaac
     8821 ttctcctaga tagtcccagc aacgcgtcat atctatttga acgagatcca caggcattcc
     8881 tgaatcaata cctgcaatga cttcttcaag tgagagggaa gctttttcta acaacgcgat
     8941 atggcgggta ttagaaacat aagtagcgtc tctttctcct gtttctccgc tgaagaataa
     9001 atcagaaata gctgattcca aacgatctag tccgtcgttt ttagctacgg aaattgaaaa
     9061 aactggttca ttttcgatca atttcttcaa tttttcttgt tccagctgtg ctggcaaatc
     9121 tgttttattt agaagaatga tccgtttcaa tccacttgtc gcttccaata attgttcatc
     9181 ttctgctgtc agtggttcgc tttggtttag aacaagtaag atcaagtctg cttctgctaa
     9241 tgcttttcgg cttctttcga ccccgatccg ttccaccaca tcttctgttt cccgaattcc
     9301 agctgtatcg attagcttga gcggtacccc ccgaacattg acatactctt cgattacatc
     9361 tcgagtcgtt cctgcaatat ccgtaacgat tgctttttct tctcttaaca aatggtttaa
     9421 caaacttgat ttgccgacat ttggccggcc gatgattgcc gtacttaacc cttcccgcag
     9481 aaccttccct tgcttggatg tagcaagtag cgcttggatt ctttgttgga tcatctgcgc
     9541 tttttcaagc aataacttcg tagtcaattc ttcgacatca tcatattctg gataatcaat
     9601 atttacttct acttgtgcta atgtctctaa aatctcttgt ctcaatgagc ggatcaaagc
     9661 agacagattg ccgtctaact gattaagagc tagtcccatt gctttgtctg tttttgcccg
     9721 aatcaaatcc atcacagctt ctgcctgtga aaggtcaaca cgaccattca agaatgctcg
     9781 ttttgtgaat tcgcctggct cagctaatct tgcaccttct cttaacaata gctgcaaaat
     9841 ttgattgact acaacgatcc caccgtgaca attgatttct actacatctt cccgcgtaaa
     9901 tgtctttggt gctctcatca cggagaccat gacttcatcg acaagctgat tgctcttggg
     9961 atcaacgata tgtccataat ggatcgtatg acttggtact tctgataatc gtttgtttcc
    10021 agattgataa actttatctg ctagttgcac agcttgatcg ccacttaaac gaacgatact
    10081 gattgcacct tctccaggtg gggtagaaat tgccgcaatc gtatcaaatt ctaaagtaat
    10141 ttcagccatt ttttcacttc ctttttcatt ttgcgcacaa aaaaagtgcc cactccaccc
    10201 tttatgcaca ataaacggtg aacttcgcac tttgactgct tgttcttgat taaattctgg
    10261 tcatagatta tcacacttgc tataaggaat caagtgttta aaacaattag atgaagttga
    10321 gcctgttctg cttccattac cttatattta attgtccttg gatgtctctt tgcgttcttt
    10381 ggtttattcg aagagaagat actgtataaa caaggcttta tctttcttaa aaaaaacggt
    10441 acactaaatg gtagcgctat ctaaactata cacttttgaa aggagaaaat atgtgagccc
    10501 gttcagatct gctgcattgg aagaagaaag tgaccctcgg atcaacgatg tcgttttatc
    10561 tacagggaat atcaaccaga aatatatcgt ccgcgatatc gtttttgttg tggaatgctt
    10621 agaagctgat ttgtttgacc catcttttcc caaagatgac cttttcttaa caacgaagag
    10681 gaaattaaag acgaaggtac ttgaatatgg tgcgaatgca gtaatcaact gtcattttga
    10741 gtatcagcaa aaaaaacgcg acggaaagta ttttctggaa ctttttgctt atggaacagt
    10801 gatccaattt atccaaacaa cgattggtta aatgaaacag acaaaaaaag cggatcaaaa
    10861 ctgaagacag tttcgatccg cttttttcta tcaaagttag tgactacttc atcgtatact
    10921 ctgataagaa tgatttctag taaaatatac ttggtttgaa aaactttgtc aacgtgattt
    10981 attcaaaaag taatggaatc aataatattt tttagcaggt tctactacta aataacgata
    11041 aggttcctca ccttccgaat gtgtttttac atgttcatct ttgcttaatg cagcatggat
    11101 ttgtttacgt tcaaatgcag gcattggttc taagaaaaca ggctgccctg tccgttttac
    11161 tttttcagcc gtgcgatcag ctaaacgctg aatgatctct tggcgctttt cacggtaatt
    11221 gcctacattg actactacag acagcttatt tcttgctaaa cgatggatga agacttgtgc
    11281 cagatattgc aatgcattca gtgtttttcc atgtttccca atcagtatgc cttgcttttg
    11341 tgtttctaaa tggaaaacaa ccatattatt tgattctctt tccattttga caagagctgg
    11401 agcatcaagc tgttttgaaa tttcagtaag ataaactgcc aattccgtta aagctgcttc
    11461 atcacctagc tcacttactt cttctgtttt ttcttcaaga atcgtttcgt tgactacctc
    11521 gatctcagct gttggttctt cttccaaaat ttcttctaca gtctgttcta ctgtttcaga
    11581 aactttttcg ctgatacttg gctggatcga aagccgtgca ttctttcgac ccattcctag
    11641 gaaacctttt tttccttctt ccagtatttc gatttcgact tggtctttgg ccaaacccaa
    11701 atcagccaga cctagttgga cagcttcgtc tattgttgca gcttcataaa tcggcattag
    11761 cattacctcc cctattattt ccgttttttc ttaggattca ttgctttctt aagagcacgt
    11821 tctctttcgc ggattctccg tgcttcttct tcacgctctt ttcgaatttt gaaagggttg
    11881 ttgatcagca gcgtttgtac aacttggaag gcattggaaa ctacccagta taatgaaaga
    11941 ccgctggcta aattgacacc catcactaaa atcattacag gcatcacgaa attcatgatc
    12001 ttcattgatg cattcgattc aatttgactc atgctagata aatacgtact tgcaaaagta
    12061 aagattgctg ctaaaatcgg caaaataaat gttggatccg cgttccctaa gttcaaccat
    12121 aagaaatgcc cttgtttcaa ctctggtaca cgtgaaatag attgccataa cgccatcaag
    12181 attggcattt gtactaataa aggaagacaa ccagcatatg gattgacgcc attttctgca
    12241 tataaacgct gctgctcttc tcttaactta ctttgtgttt ccggatctct tgaactatat
    12301 tgttgctgca gtgcttttaa tttcggttgt aattcttgcg ttttacgcat gctctttgtt
    12361 tggaaatgca tcaacggcaa taggatcacg cggatgatca gtgtaaataa aatgatccct
    12421 atccctgcat taccaaatga caacgccttg atcgcctcag caaagtaata aacaatataa
    12481 cgatcccaaa ttcctgtact ctgtgcgctg acttccccag ttccgcaacc ggataagacg
    12541 atgaccaaac taacgatacc ggccatcaaa agtaaacgtt tgtatttctt cacttactct
    12601 tttcctctct gtcgattatt tttgcaagtt ttaagacgtg gactaaattg gagcgaacct
    12661 catcataagt caaccccttc acacttggtc tagcaatcac aataaaatcc aactccggat
    12721 caatttcttc ttttatagaa taaactgctg ctcggatttt tcgtttgact tcattgcggt
    12781 ttaccgcgtt gccgaccttt tttcctacag ataaaccaac tcgaaaatga ggctgtcccg
    12841 gtttttccaa acggtaaacg acgaatttgc ggttagcaaa agaatctccc gtatggaaca
    12901 cggcttgaaa ctctcgttct ttttttactc gatacgcttt tctcattagt tttctttcct
    12961 tttctatctc tcacttacgc tcataatcat accataatta cgattgtcct ttcatcattt
    13021 ccctaaaaaa gagtataaaa aaaaccactg aaaccccagt ggcctaagct gaaagaactt
    13081 ttcttccttt gcgacgacgg ctagctaaca cacgacgtcc atttttagta ctcatacgtt
    13141 tacggaatcc gtgaactttt tgacgtttac gtttatttgg ttggtatgtt cttttcatat
    13201 attccacctc caaaatgttc ttatctattt tataaacaca gacatacttt gatagtataa
    13261 cgacaaagta gtgattttgt caatataaaa tagaagatcg attgttaaaa cttatccaca
    13321 agattatttt tttctatggg aatcctatgt ggaaaaagtt atgcacaggg attatccaca
    13381 gataataaaa ggctggtttt acacagaaaa cacacgtgta aaaaaagtaa tccacaatca
    13441 ggcgatttcc tgtgtattcc gtctcctaac tattggtata tctgtatttt ttatccacag
    13501 acgcaagagg aaatctattg gtttttacga gttttccacc tgtgcattag tggtgtggaa
    13561 aacttttttt gacaactgtg tttttctctt ttttcttttt gtcgattgtg tggataattg
    13621 taattaaaaa atttttttct tcggtttctg tggaaaacta ttgaaaaaaa tgctaaatta
    13681 gtactagtta ttcacttttt aatgaaggag gaggccttat atggtatccc tcgatgcttt
    13741 atggaatgaa ttaaaagcaa cataccaaaa ggatttatcg cctgctagct ataatacatg
    13801 gatcgaaaca gcgaaccctc gaacccttga tcaaaaccag ctcgttgttg aggttcctag
    13861 caagattcat aaagaatatt gggaaaaaaa cctggcgaca aaaatcgttg aagtcggtta
    13921 tatgttatca ggaaatgaga ttatccctcg ctttattact ggcgaagaag cagagcaaga
    13981 agaagttata gaagaaaaaa atccaaaagt tgtggcacct agtccgctaa aaaaagccat
    14041 gctgaaccct aaatatacct ttgatacgtt tgtcattggt aaaggaaacc agatggctca
    14101 tgcagcagcg ctcgttgtag cagaagatcc aggttctatt tataacccgc tcttctttta
    14161 tggaggcgtg ggacttggta agactcactt gatgcatgca attggtcatc aaatgctgca
    14221 aagtcagcct aatgccaaag taaaatatgt aagcagtgaa acttttgcta atgatttcat
    14281 caattcgatc caaaacaaaa cagcagaaga gttccgtcaa gaatatcgaa atgttgattt
    14341 actgctagtg gatgacattc aattttttgc agaaaaagaa gctacgcaag aagagttttt
    14401 ccatactttc aatgccttgt acaatgaagg gaaacaaatc gtactaacca gtgatcggtt
    14461 gccaaacgag attccgaaac tgcaagaacg ccttgtttct cgctttgcat gggggctgtc
    14521 tgttgatatt acaccgccgg atcttgaaac aagaacagcg attttacgta aaaaagcggc
    14581 ggctgaacgt ctagagatcc cagatgacac actaagctac atcgcaggac aaattgattc
    14641 taacattcgt gaattagaag gtgcgcttgt tcgagtgcaa gcttttgctg cgatgaatag
    14701 tgaagatatt tcgactagtc tagctgcaga cgcactgaaa acgttaaaat caggaaaagg
    14761 acatcctcaa ttgtcaatcc tgcagatcca agaagaagtg gcaaagtatt atcacatcca
    14821 attgaaagat ctcaaaggaa agaaacgcgt caaatctatt gttgttccta gacaaatcgc
    14881 tatgtatttg gcacgtgagc tcacagataa ttctttaccg aaaatcggag ctgaattcgg
    14941 tggcaaggat catacaacgg ttatccatgc tcatgaaaaa atccagcagc tgatggattc
    15001 tagtgtcagt atgcaaaatg aagtatcaga aatcaaaaac tttttactaa attaatttgt
    15061 cgattctatc aactgaaaat agcatgtgga taaaagaaaa aaacgttcaa aagttataca
    15121 cactttttcc acagctgttt tttcgtttct ctcttacgtt ttttgagttt tccacagaat
    15181 caacatgccc tattactttt attattcttt ttaactaata ataaataaac aaacattcaa
    15241 aggagataca tcatgaaagt tactttaaac cgagctagct ttatgcagga attgcaaact
    15301 gttcaacgag ctatttcaag caaaaccacg atccctattt tgacaggtgt aaaaatcaca
    15361 ctgacacaag aaggtttgac tttgacgggg agcaacgctg atatatcaat tgaaactttt
    15421 ttgtctgttg aaaacgaaaa agcaaatatg caaatcgaat ctactggttc cattgtttta
    15481 caagcacgtt tctttagcga aatcattcgg agacttcctg aagaaacatt tactttagaa
    15541 gttttagaaa ataaacaagt agcgatcact tctggaaaag cgaattttat cgtaaatgga
    15601 ttagatgcag ataactatcc tcatcttcct gttgtcgaaa gccataacca gatgaaatta
    15661 cctgtacacg tattgactaa actaatcaac gaaacagttt ttgctgtctc acaacatgag
    15721 agtcgtccaa tcttgacagg tgtgcatttc attttatctg ataattcttt attagctgta
    15781 gctactgatt ctcaccgtct aagtcaacgc gtgattccag tagaacaagc ggctgatcat
    15841 tttgatattg ttattcctgg aaaaagtttg atcgaattat ctcgctcatt aacaaatgaa
    15901 gaagaaatcg tcgaaatcag cattatggaa aaccaagtgt tgttcaaaac agaaacgatg
    15961 tatttctatt cccgtttgct agaaggaaac tatccagata ccaatcgttt gattccttca
    16021 agttttaata cagaggttga attttctgtt cctagctttt tagcggcgat cgaacgggct
    16081 tctttacttt ctcatgaagg ccgtaacaac atcgttcgtt tatctattcg tccagatgcg
    16141 gttgttttat acggaaattc gccagaaatc gggaaagtag aagaaagctt gagttataca
    16201 gcaagcagtg gcgatccatt agatatttct ttcaatccgg attatatgaa agcagcgttg
    16261 cgtgcatttg gagatatgag catcaaagtg aaatttatct ctgctattcg tccatttaca
    16321 ttggaaccaa cggaagatgg cgttcaattt attcagctga ttactcctgt acgtacaaat
    16381 taacaatgat tttcaatgtt cttgaaaatt taattgaaaa gaaatagaaa aaataaaatt
    16441 ttaaacagaa ggccctaaaa
//
LOCUS       DAYYAG010000062        16315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000062 DAYYAG010000000
VERSION     DAYYAG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.062"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHARQ_001708"
     CDS             complement(<1..1835)
                     /locus_tag="ACHARQ_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8410116.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHARQ_001709"
     CDS             complement(1832..2536)
                     /locus_tag="ACHARQ_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8410117.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHARQ_001710"
     CDS             complement(2548..4857)
                     /locus_tag="ACHARQ_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8410118.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHARQ_001711"
     CDS             complement(5052..5516)
                     /locus_tag="ACHARQ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410119.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHARQ_001712"
     CDS             complement(5516..6142)
                     /locus_tag="ACHARQ_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8410120.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHARQ_001713"
     CDS             complement(6149..6514)
                     /locus_tag="ACHARQ_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8410121.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHARQ_001714"
     CDS             complement(6504..6842)
                     /locus_tag="ACHARQ_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410122.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHARQ_001715"
     CDS             complement(6832..7158)
                     /locus_tag="ACHARQ_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8410123.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHARQ_001716"
     CDS             complement(7139..7417)
                     /locus_tag="ACHARQ_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8410124.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHARQ_001717"
     CDS             complement(7419..8783)
                     /locus_tag="ACHARQ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8410125.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHARQ_001718"
     CDS             complement(8796..9365)
                     /locus_tag="ACHARQ_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8410126.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHARQ_001719"
     CDS             complement(9337..10524)
                     /locus_tag="ACHARQ_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8410127.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHARQ_001720"
     CDS             complement(10588..12315)
                     /locus_tag="ACHARQ_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8410128.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHARQ_001721"
     CDS             complement(12312..12764)
                     /locus_tag="ACHARQ_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8410129.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHARQ_001722"
     CDS             complement(12876..13256)
                     /locus_tag="ACHARQ_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8410130.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHARQ_001723"
     CDS             complement(13253..13639)
                     /locus_tag="ACHARQ_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8410131.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHARQ_001724"
     CDS             complement(13641..13919)
                     /locus_tag="ACHARQ_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410132.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHARQ_001725"
     CDS             13967..14695
                     /locus_tag="ACHARQ_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8410133.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="ACHARQ_001726"
     CDS             complement(14826..15302)
                     /locus_tag="ACHARQ_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8410134.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15618)
                     /locus_tag="ACHARQ_001727"
     CDS             complement(15262..15618)
                     /locus_tag="ACHARQ_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410135.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="ACHARQ_001728"
     CDS             complement(15596..15781)
                     /locus_tag="ACHARQ_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410136.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="ACHARQ_001729"
     CDS             complement(15782..16054)
                     /locus_tag="ACHARQ_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8410137.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16251)
                     /locus_tag="ACHARQ_001730"
     CDS             complement(16051..16251)
                     /locus_tag="ACHARQ_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410138.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         4566 a         3452 c         2536 g         5761 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttt
//
LOCUS       DAYYAG010000063        16009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000063 DAYYAG010000000
VERSION     DAYYAG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.063"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            684..1487
                     /locus_tag="ACHARQ_001731"
     CDS             684..1487
                     /locus_tag="ACHARQ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8410139.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2072..2527
                     /locus_tag="ACHARQ_001732"
     CDS             2072..2527
                     /locus_tag="ACHARQ_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410140.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2646..3704
                     /locus_tag="ACHARQ_001733"
     CDS             2646..3704
                     /locus_tag="ACHARQ_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFM8410141.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3746..5695
                     /locus_tag="ACHARQ_001734"
     CDS             3746..5695
                     /locus_tag="ACHARQ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8410142.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5698..9339
                     /locus_tag="ACHARQ_001735"
     CDS             5698..9339
                     /locus_tag="ACHARQ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFM8410143.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            9739..10077
                     /locus_tag="ACHARQ_001736"
     CDS             9739..10077
                     /locus_tag="ACHARQ_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410144.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            10163..10408
                     /locus_tag="ACHARQ_001737"
     CDS             10163..10408
                     /locus_tag="ACHARQ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410145.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            10428..12584
                     /locus_tag="ACHARQ_001738"
     CDS             10428..12584
                     /locus_tag="ACHARQ_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410146.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            12596..12829
                     /locus_tag="ACHARQ_001739"
     CDS             12596..12829
                     /locus_tag="ACHARQ_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8410147.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            12819..13685
                     /locus_tag="ACHARQ_001740"
     CDS             12819..13685
                     /locus_tag="ACHARQ_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410148.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            13700..15670
                     /locus_tag="ACHARQ_001741"
     CDS             13700..15670
                     /locus_tag="ACHARQ_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410149.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         5919 a         2255 c         3214 g         4621 t
ORIGIN      
        1 cacatcgctt tttgtccacg tttgcatcgc tttttgtcca tgttcgcatc gctttatgtc
       61 caccctaaaa tctatttaag attgatataa agcctttttg gaggttgcta aagttttaaa
      121 gattaaaata tatattataa atataaataa aaggggaagc tgaagcttcc tccttttcct
      181 ttttttcaat tattcaaagt tacacaagca aaataaactc ctagatagtg ttagtttttt
      241 ttaagatttt ttatttttaa gattttgaaa taagagagga gtaatcctcc tttattaaat
      301 atctgttaat atatctgtta aatatctgtt aatatatctg ttaggggagg cttaatctct
      361 tgggagagta agtattaaag gggtataaag gtaacacttt tggacagtaa aggtaacaaa
      421 agtggacagt aagggtaaca gttttggaca gcaaaggtaa caaaagtgga catttgaggt
      481 atcacactag gataagacgt taccttcggt tttcttgatt tggaaggcga aaaaatagaa
      541 aattagatta gtaacaaatc tttgttgact gttaccttta gttgtgattt aatttaggta
      601 acaagcagaa aaaattgttt cctttaatta gtaccagatt aacatgatta aaggagcgca
      661 aaaagaagat tggagagtaa tcaatgtctc aagaagttac cgaaaagatt attgatagtg
      721 atgtagattt agtatcagaa caaacaaatg caattgtgcg ttataacaat ggtctgaata
      781 caacagcttt gcgaggattt aagcctgttg aaatggatgt gttttgggct attttatcaa
      841 aaatgaaacg tcaaggaaca gcagaagtaa cttttgattt caacctcttt cgttatttgg
      901 tcaaatacga cagaagagaa aaggacagtt tttattctgt gttaaaagat ttgagtgaca
      961 aactaggtaa tttaacctac cgttttgaag attcagacgt ctatgaacag ctgtggcttt
     1021 ttcaaaaatt ccggatagat aaacagaagg aaacagttac aattaaggca aatgaggaat
     1081 ttgaatttat cttaaactca attggcaaaa actttactcg ttttgaactt gataatttta
     1141 caaaactaag aagtagctac acaaaagaat tgtatcgtca tctcatgcaa tacagaaata
     1201 aagagtggcg aaatggttat tgggtagtca aagttgaaga ttttagaaga gcattaagta
     1261 taccttcaag ttatagaatg acaaatattg ataaaagaat actagagcag gcgaaagaag
     1321 aatttttagc tcctgatgaa aacggaatac aggtatttga aacttttgat tatgaaaaaa
     1381 tctatgcaaa gaaaggaaga agagttgatc ggctcagatt cacttttagt gagccagaat
     1441 caaacttgcc aaccatttca atgcataatt ggttagaaga agattgagaa gaagcaggtt
     1501 gtaattttaa tacaacctgc ttctttaaaa aagtatgggt aacacttttg gacaataaat
     1561 gcaagaagtt tttttgtaag caagtttcag tctaaataat atttgaaaga atggctaatc
     1621 tatacaaatt ttaacaacta tgggtaacac ttttggacaa gtcaagaaaa aaaacaattt
     1681 ttcttaattt gttgatcgac taaaggtaac acttttggac aacacagaat aaaaagtccg
     1741 gtgatatcag ttttggtagg aaacatctta atatctttgt gagattgaag gtaacatttt
     1801 tggacaagtc aactatttgt taaaagttaa tatagagtaa ttagataagt tttttctttt
     1861 cgtctgataa atggataata ttagttattt tattgctaat tagtctttca tgatgactga
     1921 aatagctaaa ataaatatct cgttggataa ttgctgatac tttaagggat atctaaattt
     1981 gctgattgat ttatttatgt tattatgata ttataataac ataaatgtgt tacgcccgaa
     2041 cctcatttta aaaaaatgaa ggggtgagac aatgaatgct attaattggc atgaatttgt
     2101 taactatatg gagagcttac tagatgatct aaaaagtttt agtattgctg atgcagtaaa
     2161 gcatgggtta ccagttagta caacgatgaa attacgagga ttgaaaattc cagatgaaat
     2221 tattaagctg agaactaaaa caattattga aaccgcaaaa gtcgttgaca atatgaacca
     2281 tgaactaaaa ggcaagaaaa caacttacga gaaagatttt gaacgttttt taaaatctgt
     2341 tattggaaaa tatctaggta aaaaatcttt cagtgaattg agagatgaag agattgtacc
     2401 tactccaatg gttagcaaaa tccgaaaaaa caaagccgag gggaattact atcttggtac
     2461 aatagcaaaa atcattgatt atttggaaaa taaagagcaa aagaaaggaa cgaagaaagg
     2521 aagataattt ttgtgttaaa tattaatggg gaagaatata ccgaaatgat tcaaaatccc
     2581 gaacctagaa ttaccgaaaa aacgtctgat tgtttgctct tttatgaaag ggggcagaca
     2641 aactaatgga aaaaaacgtc aattcaacat ctaccaaaga tcaatacagt attattggag
     2701 ggttgcttgg tgataaggat aagagaacac gtcacggatt acgcattatg cttgcaactt
     2761 ttctaggagt gcttttttta ttaacaggct tgggtagcta catttatcaa ttaggaaact
     2821 ataattatta tctcgtgaac aatacaacac ctataggctc ttcattggag tttgctcgat
     2881 caggagcaga gatcactatt tcagatgtgt ggacagatca aaacagagat gtaacagtag
     2941 taaaactcgg ttactcgcaa aacgctagaa agttgttatc tacgcaagga aaaaactaca
     3001 acttatatat ggcaacgaaa gaaaaagatc gtcctaaaaa gcttgaaatg agctatggga
     3061 tgttaagtac tgatggggat gctttcttat tcctaaaagg taaattagat gaaagagctt
     3121 atcaagtttt tattgcaaac caagtatcac taatttctgg tgatgataca gctgacgatg
     3181 ataccaaagt caatgtgaat gaagcagatt cagttacaaa agcactttct caatattcta
     3241 tgaatgatgt taataatgaa ggagtaattt cttttgggaa aaaagcaaaa cctgttaata
     3301 ctcctgataa tatcaatttc agaataaatc catattcaga ttcaactaat gtctatcaag
     3361 gaagctttct taccaaagat ggcgatattg attattcaaa agttgtagca gttacaagta
     3421 agaataatgt tgtaagtaag ttagaagatc agttaaatgc ttcaaatact aacatcaaga
     3481 aaattgaagc tacaaaagct gagtatctgg aacgtttaca acaaaatcct gacgataaag
     3541 attcgcaaga aagtattcgt caactagaca gtcgaatgga gaccgaaatt aaagcaatca
     3601 atgatacaca aaaattgatt gatcaataca aaaatgctga ctttaacaaa gattcttttg
     3661 gagacatgaa gacgacattt aagtacctaa ctctgactga ctagaagtaa attattctta
     3721 ttttagagga agtgaacaca aacagatgga aaatagtacc gagtttttta agaatattca
     3781 aactgaattt aatcatgctc tcacaccaat gaaaccaccg aaacataagt tagtgggacg
     3841 tgatgacgaa ctccatgagt tgtcactgat cttgaataga ccggaaacac cgcaagctat
     3901 cttgttaggt gaagccggaa caggtaaaac agcacttgca agtgaatggg ctgaaagaca
     3961 gcgacaagat ggaaagaaag tacaagtata caaattatca atcggacaat tgcgtgttgg
     4021 gggagatgat gtcctccaaa cacgtttaga aacattaatt ccaagattga aagaatatga
     4081 gcgaatttta aaatctgagg acaaagaaaa tgaatttgtc ctcttcattg acgagtttca
     4141 taaagttgtt agctcttttg gtagtggcaa taaaattggg ggtgattcgc taaaagaacc
     4201 gttagcaaat gaatcacttc aattgattac cgctactaca agaaaagaat atgaaaacta
     4261 tatcaaaaga gatgatgcct tagggagacg gttacaaccg ttagaattga atgaactaaa
     4321 aagcgatgtt gtcgtcgata ttttgagaca atggctaaat acatcctctg ttaaagatga
     4381 tcctcaggct tcttttaaat tatcgcaaag gattcctgat gaattacttc aagaaatcgt
     4441 acaagcaaac aagctttatc gtgaggattt gtccgaaccg gctaaatcaa ttgccgtaat
     4501 ggaggatttg attgcaaaag atcgagtaga cggaaatgct tataatcgag aaagtctatt
     4561 gcaaatattt ggtaagtata atattcagct agattctgat tttgatatgg aaaaacttca
     4621 agaggaatta aagtccataa aagggcaacc gttagcagtt tatactataa caatgttgtt
     4681 taaacgtatg aagcttggat ttgatcgttt taaaggtcga aagcaaccaa aagcaactgc
     4741 tctgtttaca ggatcaacag gggtcggtaa aactgcaatg gctaaagccg tggcaagagg
     4801 actttatggc gatgaaaata aagttctctt agttaatatg ccggactaca aaacaaaaga
     4861 tgatgcaatg cgttttagac gactcatagg tactcatgta aggcatgatc cttctgcgat
     4921 tattgtgtgt gatgaatatg agaaagctgg tgtagaaaca ctcgatgcct ttttatcaat
     4981 tttagatgaa ggtaggataa gttttactga aagaggatgg gatgatcgag aagttcacga
     5041 cactgtttca ttgtcaaata caattttatt tgcgacaaca aatgcaggtg cggagatgtt
     5101 cgattcgtta aacagatata gctcactatc tcgtgttaca aacaaggaaa attctaatga
     5161 atatagcact gaactaaaaa gagaataccg aaaagtcgca tcagggatca aacaagcctt
     5221 gattggtgaa ggtctaaagc ctgaagttat taaccgattt ggacgaatag ttccattttg
     5281 ggctttatcc gaacaaactc ttctggaaat tgcttataat gaaatgttga agagtgctga
     5341 attagtacga aaacatttaa atatagattt acgtcttcca cctaggaaag aaagagtata
     5401 tggtcaaaat ttttatgcag acgatctctc catgcactta gtttttgaca tggcttcaca
     5461 agaggattct aaatcagggg gtgcaaggaa tatcttgcat ttagttgaat ctgagtttga
     5521 agaagcgatt actgaagcaa taattactta tccaaacgca aaaatattcg atgttaagtt
     5581 gagtgaagcc ggagcgtttg aaggaaaagg tgattctaat gctgaaatta ttgcaaagcc
     5641 ttccgttgct gaaagtaaag aaaacaagat tgtattttcg attggagggg cgtagctatg
     5701 aaattcaaga aatatttact acaaaacctt agaataaaac gatgggtcgt aagaggtcta
     5761 gttattggag gagtattagc agttacttta ggcgctcctt ttggaatgaa tgatccaact
     5821 cctgacaatc aagatagcaa acaagaaaca gttataaata agaacgttgc atatgcggaa
     5881 agtaaaccgg cagaacaagg aatgtggaca ctatatgctc gtatcctaat gggagataaa
     5941 gaagttaatg atagtgccgc taaaactgga caaatgaatt tgttaggaca aggtggtatc
     6001 agtgccgatt tcccttattc tgacatttct aaacaaggta attcgattgc agggggtaat
     6061 gcaggtactc aactagcatc tactttagca acttatagtt actacggata tattgaaaca
     6121 gtctcaggaa atgctattgc cagtcaagca tcatccattc taggaagtat tggtaggacg
     6181 attggtagtt ttatcggatc aattagttta atcctttcta atattgctga atccataaat
     6241 gaactccttg gaaaattttt catgagtatg aatatatttt ctcttatggg gtggggagaa
     6301 ggtggatcgg atgtatctaa tccggtatct aaagcattaa ctagcttgct taacaatttg
     6361 ggattaaata atgaattatt taaagcgata gcaagcttat ctatgctctt tttcacagca
     6421 atactaggtt atcaaggaat gaaattattg tcgaaagatg gtgttaactc gaagggatta
     6481 tcaccagtac ttgaaaaatg gattgttcgt atttttgtat tgtttatatt cttaccagtg
     6541 gcaggatcgc tattgggagt ttatatcaac ggcatccagt cagcaaaaaa agatggagat
     6601 ataccaacaa atgttgtttc tgactacatc attaacacaa gactttgggc ttctactcaa
     6661 aatcttgctc catcatctga tactggtggt gatgtacctg acggaaagcc tacatccgaa
     6721 atcaagttcg ttgatacaga ttatgaacca tctaaaaaat ctgcaatgat aagagcgatc
     6781 aatactagca gttatgccag acaaaataat gatctagatt ctaattcagt cggtatggct
     6841 ctattaaaag aatggtcatc gaatgatact ttcaatatta atacttatgc cggtgatgtt
     6901 aatgtcgccg aaaatgatgc ttatatggga agagcgcctg ctgtttggac aggctataat
     6961 ttaggggatg gaaaagaagg aagtgcaatt aagaaaccaa ccaatttaaa tggatatatt
     7021 tggtcagcaa atcaaaatgt gacagacgac aatcgcaagc ctgatggaaa aggaacagcg
     7081 cataactttg atgctactca accttggggc gttgctaaca atgcaacttt tagcacacag
     7141 tcggtagctt tattgctcca atcggcactt gatacatcgg gagctaagtt ttatgcgtat
     7201 aatatcgctc cttcaggaat tcaaggcagt gctaagaata tgtcaacagt aaaaacagaa
     7261 tggcgtacag catccttagt tggtcatgga gctatgggta agtttggctc gtggctagcg
     7321 atgaacgctg aatcaatcgt tttagcagtt ctttacttag catgtgcttg ggcgatgttc
     7381 aatgtgaata tcgtgaaagc atttaaacgt gaatttattc ggatttggga agtagttcta
     7441 acaggatcac ttgcatcagg agcaggtgtg atggcaattt cattaggtgt tgttacaaca
     7501 tctctgatcg gactacaatt acctatcttt ctgattactt ttattcgtgc atttggtgat
     7561 actgctgtgg gattcattcc taatacatta gttggcgatg gcgtagcaaa tattgttcga
     7621 tctgctatga ttttaggtgt aggatatctt ttagtcttta aaaaaggcga aaaagtaaat
     7681 aatttgatta atatgcttat tggcttacca cttgaagtag cgtttgggtt tgatgaacgt
     7741 gctaagttat tatttggtaa tagaaatgat gctgtgcgag ttgtggacgg cgtttctaaa
     7801 gcaatcaaga aaagccaagg agctaaaaat ccctattatg atggtggcaa cagctcagta
     7861 aaagatatgg ttgcaggagg tcttggagca gttggagcgc aaaaaatggc tgaacgtttg
     7921 aacaatatgg gagatagcaa tgctgatgct aagtccattg ctgataaagt aattcctgaa
     7981 acaccagaag gaatgtcatt caacggtggc ggagttggtt ccacaatcaa taatacctct
     8041 ttcaaagatt tagttcgtca agatcaacta cgtaataact cccaacaata tattgatcat
     8101 ttaggacgta tggttgatga aaacggaaac tttgtcgatg aaaatggtga aatattaaaa
     8161 gatagtaaat acggtaaaga tgctaaaggc aactttattg acgaaaaagg gcgtttggtc
     8221 gatgaagatg gaaaccttat agatactaat ggtaaaccat tggaagatag tgaagatggt
     8281 aatattggta tggatagcga aggacgttta attgatgaaa aaggtcgctt agtcgatgaa
     8341 gatggaaatc ttgttgacga aaatggacgc aggttgctaa atggtgattt tggtcatgat
     8401 agtaacggac gtttagttga cgaaaaaggt cgtttagttg atgaagatgg aaatcttgtt
     8461 gatgagtttg gtaataaact tggtaaacac agcgatggat ataatgtgaa tggagattta
     8521 gtcgatgagg atgggaatat cattgagacg agagatgcgc ttgtggcaga tcgaaataga
     8581 gaactcgaac aacaacggca tacgccaatt gctgttgcaa ttcctgagaa tgaagaagac
     8641 gagattactt tagatcaaac tcatcaagaa ctgaggaatg aaatgagtgg tgaggacgtg
     8701 tcagacagag tgcatcggga attaggagat ggagcatctg gtgaaagtca gtcaagagag
     8761 cttaacagca actttgataa tagtgctatt cggaatgaca cagtacttaa agacagagaa
     8821 tcgctaacaa aagaaacgga taaagaacaa tcagatgtga agaaaaagtt cattcctact
     8881 cctaatgtta atcaaacaga taaatatgaa acaaatattg gatcaggaat gtcgaaagac
     8941 aatcaatcaa ttagagaacc tgaactgaca aataaaaata atagaacaaa taattctaaa
     9001 tctacttcag ataattcagg gaaatctaat caatcaacca agaaaacatc aacagctaaa
     9061 tcagccgttg aatatctatc aaaaactggt aaaaatgttt acacctcagc agataaagct
     9121 ttagcaaaag ctccaaaagg tagtctagca gggataacct atgaagctaa aaacgaagct
     9181 attaaaatag gtaaaggaac ggctaaggtt gccaaggcta ctgctacagg agcatccaaa
     9241 actggtaaag tagtgaagaa atctaccatg tcagtcaagg actatattgt aaaatacggt
     9301 aatcaaaaat caactgaaaa taagtctgaa gacgagtaaa aagaaggagc attgatttgc
     9361 tccttctttt tactttgatt tctaactgaa gatgtcaagg aaaatttcta tttatgcaaa
     9421 aaaattattt taaattgtct ataaatgaat taaatattta ctgttcaaag ttgtataatt
     9481 aaaaagggct tatgctggta aagaacagaa ttattatcta aacaaaaact tgataaacca
     9541 gcatttttag ccaataaaaa aagtgaaata atttaatgga aaatctcttt taatcatcga
     9601 aaatttatgt tattatgata acataaagat gtaaatatcg agacgtcttt ttcttagtta
     9661 gatcattgac aagtgaacaa ataagatttg tcgatgatat aattaactta ttaaaaatat
     9721 taaaaaggag gtttttatat gttcgattct attaaaaatt tggttagcgg aatgacgcct
     9781 gatatgaaaa ctttagtaca agttgttttg tatttcttgg cattcgtttt ttcgttgaaa
     9841 ccaggattca ctgcaatgaa attttttggt gatgcaaaat ggggagatgg attaaaatat
     9901 gttggggcga ttattgcaat ctttattatt cctgtaatta caaccgttat gctttacaca
     9961 atgggtacta aaacaggtaa tgatttgaac caaaaagcta atatcatatc agcacttatt
    10021 ccagttgttg gtatctatct agtttcaaaa tttccaaaaa atgaactgtt aggttaaaag
    10081 tgaattgaac aaatttggat aatatgccga tttgttcaat ttacttttta ccaaaaaact
    10141 acgctccaag cgtagtttag aagtgaggga agaaagtaaa tgtaagatgg tttttgatag
    10201 agaaaaaatg ttagctcacg ccaacgaagt attaatgagc agtctgaaag gtaccgaact
    10261 ggcaaagata atgaatatga atgttaacca gttctatgat tatcgaaatg ggagtaaaaa
    10321 gattgaaaaa gccagacttg aaacgcttat taaattcgaa aaagcatatg tatatatgct
    10381 agataaacag aaaagaacta ttgactgaaa aaaggagttt tacaataatg gcaaaattca
    10441 aagatattac cgaatctatt aattatgtgg cagaaacagt tcctgtagtt gcactctctt
    10501 tgaagccaca attactcgaa gtgtacaaaa aagaagaaaa agaagcgaag gcaaaaaaag
    10561 ctaaagcaaa ggtatggaaa gaattcgatg cgttgctgac caaagattta ccaaagaaaa
    10621 attatatggt aattgaagat gtacaaaagc tcaacagtga gggagttata gatgaggatg
    10681 atgctattct cgattctgag aagtatgtcg ttctattgaa gtcaagtttg aattatgtag
    10741 ctgatctacc agaaaaattt aaagagaagc tattagcagg tgtccgtttt atggatagta
    10801 gtggtcaatt attagttatt ccggatgatc ctaatgtgtt tgaatcagaa gttgaagaag
    10861 ctacagactt ctatagcgca gtagagcaat tagaagtagc tactaatgga ttagaacgaa
    10921 ttgctgagac cttcaactct tctgaagaag ttgaatttga agccgtagca gaaaatgtac
    10981 cagatgaaaa agaagctctt tttgtggatg ataacaaacc aaccgaagca gaaaaccaag
    11041 aagtaagcat agaagaaaca caaatacaaa cagaaaccta ccaacaacca aaaattgatg
    11101 aaaaacccga atcccaaagt gacaaagaaa attatgttta tgaagctgaa aaaatcacat
    11161 ctgaaagtga tgtaccgaaa aacttagatg ctgatttttt agaaaaaaat tttgaacaga
    11221 tcattgctga caaatttcaa cctgtagttt tagatgagat taatgaccct gtcgatttaa
    11281 acgaggagat gctatccata cctgagttga aaccattgct gtttttagct aaagagagta
    11341 tgaacgctcg catctctatt gctaataata aaatcaacag tgaattaaat aagacgaagg
    11401 agcaattgat cctcaaagga aagcaaatgt tagctgatga gcttttgcgg atagaggaag
    11461 caacctcatt aaatggtcaa aatcagttta caaatacact atcggttgct aaagagagct
    11521 acgaagcaga agcatctaaa attgatgatt tagtgatgga aaaatccaat atcattcgta
    11581 aagattatga tatggcgaaa caggcttatt tggatgctga actaaagaaa ctagagcaac
    11641 ggtatgatgc tgagcattta ccagaagtta aacaacaaga agatgaatat agaaaagcaa
    11701 ctgaagaaag tcttgatcaa cagtaccaag aagcaattga atctattaaa aacgatgcat
    11761 tgaaacaaag ccaagaagca aaagaagttg tggtggaacg aattatcaat tcattgaaac
    11821 cagaaattga aaaatcatta acagacgtgc aagaaaaaac tttagagatt gtcgaaaaaa
    11881 caaagtcaga aaatgaagct gagttccatg atacaaaaga gaaaacaaca gatgtggttg
    11941 tcaaatattt tgcgaacaaa gaagaagttg aaaagcaggt agctaacaac acgaaagaat
    12001 tagaaacaaa acttgtagag caacaaaaaa ctatcgaaga atataaagat cgaatgaaag
    12061 aggtcaaaga tttagcagag ggttataaat taaatagcga aaagttggca gaacaaagtg
    12121 aagctttaag agaagactta tctcgttccc gaagtgatct agctcaaatg gcagaaaaaa
    12181 cgaaatcatt agcagttgct acagaaaatc aatcaagttc aaaacgtgat tttacaagta
    12241 aaaagatggc tactatcatt tctgtcgctg caatggcttg cggaacgatc ttgggatcgt
    12301 cctactttat tagtcataat aatcaacaag cacaggctca aactcaaaca tctatttcga
    12361 gagtggaaaa ttcgattggg acaaatacga actctgcttc aacaaatagt agctatgaag
    12421 caacaccaac tacagagttc aaagttggga aagatgttgt catcagtatt gatgggaaaa
    12481 caactaaggc tgaagtgatt tctattgatg gagaaaaagc agttgtgaaa actccagacg
    12541 gaaatcagta caaaattcaa gctcacttat ctgataataa ctaaggaggg ctattatgcg
    12601 agaagaaaaa atagtttatc cagtaattca aaacaatcaa gaaaacatga cctcttctca
    12661 gcacttacag gaagcacagc tcgtgtatca ggtaaatgaa ctaccacaaa cgaacgagac
    12721 aatgagcaag tcaatatctt taattggctt gcttattgtt gcaatagtga taactgtagg
    12781 tgtttaccgt aagttgaacg tgaaaggaag tatttctgat ggcagataac tcaaactatg
    12841 gatttaaaca taacttacat caaaaacctt ctgaaacaat tgtcagagtt gaatggcttg
    12901 aaaagcatgt caatagcacc tattctgtta cagcaaaaag attaattgta tcagctatag
    12961 ttgttgcagt cgttgttctt ggatcaattg tttacttagt ttctgatacg attaaactga
    13021 aatttatttt tgttccaacg ttattaatga taattggatt tcttattcta attagccagt
    13081 atatgtttat tgatccatct agtggttcat tgatttctgg ttctattaga ctgttcaaat
    13141 cagtagttaa ttcagcaaga gtatctatgg ggaaaaagaa accatttcga ggcacagaga
    13201 ttttaaaagt gaaacaagat ggaacaatcg tatatcgaga tgggagtgta gcattaatgt
    13261 tcaatctaga tggggctaca tcactaaccg cttttccaga agaagttatg gctcaggaat
    13321 cgaaatcaat acaatatcaa aataccagag atcgttcctt agctgaaatc aaaatcactt
    13381 catctcaacc tcagaatacg gaaagacaaa ctcgaaatat gaaaatacaa aaacgaaatg
    13441 caaggagtaa caaagcagtc tttgacataa tagatcagca agaacgattt gtatcagaat
    13501 atattgatgg tgtgaaaaca acagtaatcc aatatcttat cttgaaatct caatctaaga
    13561 tggtattgga tgaaggtgtg gaacgtttag aaaactatac gagtagagga ctttattata
    13621 gtgtcactca gttgtcagct aaacaaacaa aagaaatatt atctgatatt tatgcactga
    13681 aatagaggtg agagattaaa tggaaattat gggtagatca caactattaa atttcaaaaa
    13741 aaagagaaaa actaaaaaag aacaaaaaac tagaaattca aaggtagtga ataatacctt
    13801 tgttagtgat ttaacatctt tagttggtta tcaatttaca ccatcttaca ttaggacagg
    13861 taagcggtgg ggaacaattg tgaaattagt gaatacttat ggaatgaata gaaattatgg
    13921 atttggttgg atggttaaag ctattcctga agtgagagta gaaggggtaa aagcctatct
    13981 aattgaagca gataagccag tgtcacaaaa agatcaacaa gaaataatgc acgagaatgt
    14041 tgggggatca acgaatagtt atgaaaataa tttagaagga aaaaattcag atgctgaaat
    14101 tagcaaaact gttatgcgga ttatggattt gaatacagca tctttactag acagcatggc
    14161 aaataaaatt attgattgga agctagaaat cctattagta tccgattctc ctaataaaat
    14221 cagcgaacag ctaattaaac tccaacggtt gtatgatgaa aaaatgtatg gattaaagct
    14281 tatgtcatat ggaggagatc aacgtgatct gtttgataga cttttaagtg aaccaatcgg
    14341 gaatgtctat gagtatacgt ctatgagttc ggattttgca ggaaatgatc atgcaattcg
    14401 caaaggttta gatgatgcga atggatgggc tatcggcact atgtctacta gctacgcaag
    14461 tggtgtagct tttatggcac tggatgattc ttttacaagt aaagttttag tggcatcaca
    14521 caaaaacgct acaattaaag gctatgatga aaaaatctca gcatcatcac tttgggggca
    14581 attgattgct aataatgcaa tggcaaatca taaacgaact tttcatattg tcttaaataa
    14641 atttgattac tttggtgatc caactggtag aacatttgtt gccaaacctt cgttaaacag
    14701 tgtaatagaa aaggttaatt tggctgaaga aggtcttaac tctttggagc tttttggtaa
    14761 tcaaaaggac attggtgatt tatatagacg gaatctcgat aaactaactc agatttttta
    14821 tttgttagca ggcagaacat tagattctgc aactagagtt ttattgaaaa aagaattagg
    14881 taacttttat ataagtcaga aactttggga tattaattat gaagaacgtt caaaactttt
    14941 ggtgaacagt agtgatacaa tgccagttct tggggatttt attagctatt tgactaatgc
    15001 tgaaacaaaa gccaaaaaat ctggtactga aaaagaagtc gatcgtatta ttgtattaaa
    15061 agacacattg agaacagcat tagacagctt ctctaggatg tttaatcaaa gaacgacact
    15121 gaaagaaatc ggtaaaagtg acaaattaca gttttactat aacttaggtg atctaagagc
    15181 agaaaccaat gttatggaag ctcaattttt aaataccttt gatttcatca catcacaagc
    15241 aaaaaaagat gatattgtta tgattcacgg cttcgataag ttatctattg aaaccgttgg
    15301 gctaattaaa aaaagattaa caatgcttga agaagatgga attcgtcttg cctttctatt
    15361 tgatacaatc ggttcaggag aagacaatga atacaaagaa gatattgaaa cagctgatat
    15421 attcaataca gatggaaaaa tttaccaaag ttttgaaagt gattttaatt acactatcat
    15481 tggaaagatg accagtggag agttaatgaa gtatgaaaaa aaagttgctc ctgctagaaa
    15541 aggagaagat gatcaaaaac tttctgttga tttgagacag tatttgacaa caggaagaag
    15601 taataaattt cagatcagga gaccagtgga tctgactaat aattttgtta ttggagagtt
    15661 tattctttaa gttttatctt tgattttgag tgtataagga aaggataaga aaaaacatgt
    15721 atatttgaga ctaaaaacag tcttagagaa catggtacgt tttgtgaaaa gagaactata
    15781 gaaatttcat aaagtgaaac cttactgagt gaaataatta tagtggaggt taaataatga
    15841 aaccaaacat aaaaatttta gatcgaatat ttctaggtag agacacagaa gtaatcctaa
    15901 ttcaacatga agaaggattt gaagttagta taggtattca gaaacttcaa aaacctcatt
    15961 attgtaatca gttgtataaa aactttactg atgaagaaaa agcgagagt
//
LOCUS       DAYYAG010000064        15740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000064 DAYYAG010000000
VERSION     DAYYAG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.064"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1499
                     /locus_tag="ACHARQ_001742"
     CDS             72..1499
                     /locus_tag="ACHARQ_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410150.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1591..1956)
                     /locus_tag="ACHARQ_001743"
     CDS             complement(1591..1956)
                     /locus_tag="ACHARQ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8410151.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1953..2318)
                     /locus_tag="ACHARQ_001744"
     CDS             complement(1953..2318)
                     /locus_tag="ACHARQ_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8410152.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2510..2758)
                     /locus_tag="ACHARQ_001745"
     CDS             complement(2510..2758)
                     /locus_tag="ACHARQ_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410153.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2757..2885
                     /locus_tag="ACHARQ_001746"
     CDS             2757..2885
                     /locus_tag="ACHARQ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8410154.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2942..4636
                     /locus_tag="ACHARQ_001747"
     CDS             2942..4636
                     /locus_tag="ACHARQ_001747"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8410155.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4736..4909
                     /locus_tag="ACHARQ_001748"
     CDS             4736..4909
                     /locus_tag="ACHARQ_001748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410156.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5287..6420
                     /locus_tag="ACHARQ_001749"
     CDS             5287..6420
                     /locus_tag="ACHARQ_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8410157.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6451..6771
                     /locus_tag="ACHARQ_001750"
     CDS             6451..6771
                     /locus_tag="ACHARQ_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8410158.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6800..7264)
                     /locus_tag="ACHARQ_001751"
     CDS             complement(6800..7264)
                     /locus_tag="ACHARQ_001751"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8410159.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7396..8649)
                     /locus_tag="ACHARQ_001752"
     CDS             complement(7396..8649)
                     /locus_tag="ACHARQ_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8410160.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     SPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8678..8995)
                     /locus_tag="ACHARQ_001753"
     CDS             complement(8678..8995)
                     /locus_tag="ACHARQ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8410161.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9021..9341)
                     /locus_tag="ACHARQ_001754"
     CDS             complement(9021..9341)
                     /locus_tag="ACHARQ_001754"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8410162.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9805..11220)
                     /locus_tag="ACHARQ_001755"
     CDS             complement(9805..11220)
                     /locus_tag="ACHARQ_001755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8410163.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11237..12553)
                     /locus_tag="ACHARQ_001756"
     CDS             complement(11237..12553)
                     /locus_tag="ACHARQ_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8410164.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12703..13401)
                     /locus_tag="ACHARQ_001757"
     CDS             complement(12703..13401)
                     /locus_tag="ACHARQ_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8410165.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13690..14679)
                     /locus_tag="ACHARQ_001758"
     CDS             complement(13690..14679)
                     /locus_tag="ACHARQ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8410166.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="ACHARQ_001759"
     CDS             complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="ACHARQ_001759"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8410167.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4897 a         3192 c         2692 g         4959 t
ORIGIN      
        1 tgatttcaaa aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa atacaaggcg
       61 gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag ctgtttttat
      121 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
      181 tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga
      241 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      301 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      361 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      421 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      481 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      541 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      601 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      661 aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      721 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      781 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      841 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      901 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      961 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc
     1021 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt
     1081 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1141 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1201 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1261 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1321 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1381 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1441 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1501 gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1561 aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1621 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1681 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1741 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1801 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1861 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa
     1921 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1981 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     2041 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     2101 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2161 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2221 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2281 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2341 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2401 tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat
     2461 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2521 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2581 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2641 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2701 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2761 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2821 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2881 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2941 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     3001 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     3061 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3121 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3181 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3241 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3301 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3361 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3421 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3481 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3541 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3601 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3661 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3721 agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata
     3781 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3841 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3901 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3961 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     4021 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     4081 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca
     4141 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4201 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4261 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4321 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4381 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4441 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4501 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc
     4561 ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4621 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4681 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4741 agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4801 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     4861 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     4921 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4981 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     5041 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     5101 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5161 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5221 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5281 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5341 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5401 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5461 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5521 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5581 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5641 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5701 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5761 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5821 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5881 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5941 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     6001 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     6061 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6121 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6181 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6241 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6301 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6361 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6421 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6481 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6541 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6601 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6661 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6721 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6781 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6841 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6901 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6961 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     7021 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     7081 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7141 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7201 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7261 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7321 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct
     7381 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7441 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7501 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7561 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7621 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7681 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7741 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     7801 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     7861 gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     7921 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     7981 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     8041 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     8101 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8161 tgccggtgac acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8221 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8281 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8341 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8401 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8461 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8521 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8581 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8641 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8701 gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     8761 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     8821 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     8881 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     8941 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     9001 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     9061 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9121 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9181 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9241 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9301 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9361 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9421 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9481 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9541 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9601 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9661 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9721 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
     9781 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
     9841 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
     9901 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
     9961 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
    10021 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
    10081 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10141 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10201 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg
    10261 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10321 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10381 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10441 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10501 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10561 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10621 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10681 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10741 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    10801 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    10861 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    10921 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    10981 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    11041 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    11101 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11161 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11221 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11281 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11341 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11401 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11461 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11521 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11581 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11641 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11701 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    11761 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    11821 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    11881 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    11941 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    12001 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    12061 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12121 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12181 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12241 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12301 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12361 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12421 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12481 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12541 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12601 gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12661 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12721 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    12781 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    12841 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    12901 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    12961 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    13021 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    13081 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13141 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13201 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13261 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13321 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13381 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa
    13441 ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt
    13501 catagacata caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc
    13561 tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc
    13621 ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt
    13681 acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa
    13741 attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac
    13801 acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc
    13861 tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc
    13921 ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag
    13981 ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc
    14041 ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg
    14101 catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt
    14161 atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg
    14221 catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa
    14281 gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga
    14341 tttacgagag ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga
    14401 aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt
    14461 ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat
    14521 gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt
    14581 ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata
    14641 ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat
    14701 ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt
    14761 aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca
    14821 gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt
    14881 gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg
    14941 gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt
    15001 gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc
    15061 caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta
    15121 tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta
    15181 cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga
    15241 tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg
    15301 cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct
    15361 tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc
    15421 ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat
    15481 tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg
    15541 gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc
    15601 ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa
    15661 taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa tccttatcga
    15721 cagattctcc tttttaatca
//
LOCUS       DAYYAG010000065        15206 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000065 DAYYAG010000000
VERSION     DAYYAG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.065"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHARQ_001760"
     CDS             complement(93..1136)
                     /locus_tag="ACHARQ_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8410168.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHARQ_001761"
     CDS             complement(1513..2478)
                     /locus_tag="ACHARQ_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8410169.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHARQ_001762"
     CDS             2712..3557
                     /locus_tag="ACHARQ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8410170.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHARQ_001763"
     CDS             3748..4935
                     /locus_tag="ACHARQ_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8410171.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHARQ_001764"
     CDS             4941..5297
                     /locus_tag="ACHARQ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8410172.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHARQ_001765"
     CDS             5299..5637
                     /locus_tag="ACHARQ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8410173.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHARQ_001766"
     CDS             6032..7066
                     /locus_tag="ACHARQ_001766"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8410174.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHARQ_001767"
     CDS             7059..7745
                     /locus_tag="ACHARQ_001767"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8410175.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHARQ_001768"
     CDS             7769..8617
                     /locus_tag="ACHARQ_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8410176.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="ACHARQ_001769"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="ACHARQ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8410177.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="ACHARQ_001770"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="ACHARQ_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8410178.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="ACHARQ_001771"
     CDS             10883..12118
                     /locus_tag="ACHARQ_001771"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8410179.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /gene="sufU"
                     /locus_tag="ACHARQ_001772"
     CDS             12105..12575
                     /gene="sufU"
                     /locus_tag="ACHARQ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8410180.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="ACHARQ_001773"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="ACHARQ_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8410181.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..>15206)
                     /locus_tag="ACHARQ_001774"
     CDS             complement(14069..>15206)
                     /locus_tag="ACHARQ_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8410182.1"
                     /translation="TFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAEAQ
                     TALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAF
                     GELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLP
                     RKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDID
                     YFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILY
                     GYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFES
                     GASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4973 a         2836 c         3250 g         4147 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgtt
//
LOCUS       DAYYAG010000066        14961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000066 DAYYAG010000000
VERSION     DAYYAG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.066"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHARQ_001775"
     CDS             5..2377
                     /locus_tag="ACHARQ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8410183.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHARQ_001776"
     CDS             2438..3898
                     /locus_tag="ACHARQ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8410184.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHARQ_001777"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHARQ_001777"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8410185.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHARQ_001778"
     CDS             6129..6278
                     /locus_tag="ACHARQ_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410186.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHARQ_001779"
     CDS             6353..6946
                     /locus_tag="ACHARQ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8410187.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHARQ_001780"
     CDS             6959..7858
                     /locus_tag="ACHARQ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8410188.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHARQ_001781"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHARQ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8410189.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHARQ_001782"
     CDS             8724..8984
                     /locus_tag="ACHARQ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410190.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHARQ_001783"
     CDS             9429..9635
                     /locus_tag="ACHARQ_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8410191.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHARQ_001784"
     CDS             9656..9886
                     /locus_tag="ACHARQ_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410192.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHARQ_001785"
     CDS             9902..10339
                     /locus_tag="ACHARQ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410193.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHARQ_001786"
     CDS             10332..11039
                     /locus_tag="ACHARQ_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410194.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHARQ_001787"
     CDS             11203..11403
                     /locus_tag="ACHARQ_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410195.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHARQ_001788"
     CDS             11419..11598
                     /locus_tag="ACHARQ_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410196.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHARQ_001789"
     CDS             11923..12186
                     /locus_tag="ACHARQ_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8410197.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHARQ_001790"
     CDS             12180..12530
                     /locus_tag="ACHARQ_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8410198.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHARQ_001791"
     CDS             complement(12554..13168)
                     /locus_tag="ACHARQ_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8410199.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACHARQ_001792"
     CDS             13482..13904
                     /locus_tag="ACHARQ_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410200.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACHARQ_001793"
     CDS             13914..14117
                     /locus_tag="ACHARQ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410201.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACHARQ_001794"
     CDS             14328..14912
                     /locus_tag="ACHARQ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8410202.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5512 a         2429 c         3035 g         3985 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg a
//
LOCUS       DAYYAG010000067        13968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000067 DAYYAG010000000
VERSION     DAYYAG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.067"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1330)
                     /locus_tag="ACHARQ_001795"
     CDS             complement(146..1330)
                     /locus_tag="ACHARQ_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8410203.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1355..2362)
                     /locus_tag="ACHARQ_001796"
     CDS             complement(1355..2362)
                     /locus_tag="ACHARQ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8410204.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2534..2893)
                     /gene="comGG"
                     /locus_tag="ACHARQ_001797"
     CDS             complement(2534..2893)
                     /gene="comGG"
                     /locus_tag="ACHARQ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8410205.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2865..3290)
                     /gene="comGF"
                     /locus_tag="ACHARQ_001798"
     CDS             complement(2865..3290)
                     /gene="comGF"
                     /locus_tag="ACHARQ_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8410206.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3253..3600)
                     /locus_tag="ACHARQ_001799"
     CDS             complement(3253..3600)
                     /locus_tag="ACHARQ_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410207.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3572..4042)
                     /gene="comGD"
                     /locus_tag="ACHARQ_001800"
     CDS             complement(3572..4042)
                     /gene="comGD"
                     /locus_tag="ACHARQ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8410208.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4017..4295)
                     /gene="comGC"
                     /locus_tag="ACHARQ_001801"
     CDS             complement(4017..4295)
                     /gene="comGC"
                     /locus_tag="ACHARQ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8410209.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4292..5341)
                     /gene="comGB"
                     /locus_tag="ACHARQ_001802"
     CDS             complement(4292..5341)
                     /gene="comGB"
                     /locus_tag="ACHARQ_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8410210.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5298..6227)
                     /gene="comGA"
                     /locus_tag="ACHARQ_001803"
     CDS             complement(5298..6227)
                     /gene="comGA"
                     /locus_tag="ACHARQ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8410211.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6359..8113
                     /gene="ade"
                     /locus_tag="ACHARQ_001804"
     CDS             6359..8113
                     /gene="ade"
                     /locus_tag="ACHARQ_001804"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8410212.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8211..8873)
                     /gene="trmB"
                     /locus_tag="ACHARQ_001805"
     CDS             complement(8211..8873)
                     /gene="trmB"
                     /locus_tag="ACHARQ_001805"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8410213.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8943..9725)
                     /locus_tag="ACHARQ_001806"
     CDS             complement(8943..9725)
                     /locus_tag="ACHARQ_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8410214.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9929..11140)
                     /locus_tag="ACHARQ_001807"
     CDS             complement(9929..11140)
                     /locus_tag="ACHARQ_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8410215.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11133..11870)
                     /locus_tag="ACHARQ_001808"
     CDS             complement(11133..11870)
                     /locus_tag="ACHARQ_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410216.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12008..12442
                     /locus_tag="ACHARQ_001809"
     CDS             12008..12442
                     /locus_tag="ACHARQ_001809"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8410217.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12435..12779
                     /locus_tag="ACHARQ_001810"
     CDS             12435..12779
                     /locus_tag="ACHARQ_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8410218.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12959..>13968
                     /locus_tag="ACHARQ_001811"
     CDS             12959..>13968
                     /locus_tag="ACHARQ_001811"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8410219.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4435 a         2862 c         2357 g         4314 t
ORIGIN      
        1 caagcaaaat tccctaagaa gatcctgtaa aaaagaaggg aaattgaagt taaattacca
       61 ttttgcggtc ttctccgcaa aaaaaacaca tgcaagaatt ccctcttgca tgcgtttcat
      121 cttttatcct aacgaatgct ttggcttagc cgcgcagacg ctccacatca cgggcaatca
      181 ttagttcctc atctgttggg atcactaata ctcttacttt tgattctttt gtagagatat
      241 ctacttcttc accacgcaat tcattcaacg ctggatcgat ttcacaacca aaccaagaca
      301 tacctttgat gatttcacta cgtacatgtc cgtcattctc gccgatacca gcagtaaaga
      361 caattgcatc aacaccattc atcgttgtca cataaccgcc gatgtatttt cggatgcggt
      421 ccacgaaaat gtcataagct aaacgaatat gttctttttc atagttcttt tcaagatcac
      481 gcatatcact agacaatcct gttaggccta gcagaccaga tttcttgttc aaaacattga
      541 tcatttcatc aatatcaatc tctaattttt ccattaaata tgggagaaca gctggatcga
      601 tatcacctga acgtgtaccc attgtcaccc cagctaatgg tgtgaatccc atagaagtat
      661 ccatggattt acctccatcg actgctgtga tcgatacacc atttcctaag tggcaagtga
      721 tgatttttag ttcttcgatc ggtttgttca ataattttgc cgcacgttcc gacacataac
      781 gatgacttgt gccatggaag ccatattttc ttactttgaa atcttcataa taagacaccg
      841 gtaagctata taaataattt tgctttggca ttgttgagtg gaaagatgta tcgaatacag
      901 ccacactcaa aatgtcaggt aaaatctttt taaacgcttt gatccccatt aagtttgcag
      961 gattatgcaa aggagcaaat tctgctaatt cagcaatgcg gtccattact ttctcatcga
     1021 tcactactga atccccataa gtttctcctc cgtgaacaac acggtgccct actccggtga
     1081 tttcatcata agagcttaaa atttgcagtt caatcaactg atctaacagc atttttactg
     1141 ctacttcgtg gttttcgata tccatgattt gttcgaattt ttgattatcg ccgtacttga
     1201 tggtaaaaat agaatcttgt aaaccgatac gttcgacgat ccctttcgca actacggttt
     1261 cttctggcat ttgatataat tgccatttca aacttgaact cccagcattg attgcaattg
     1321 ttttagacat atgactctcc ttctcgttga atgattacag agcagatgat ttccagtttt
     1381 taaactcgtt gaaaaattcc gtcaccttct ctggttcttt taatgaagca agtttcacaa
     1441 gcaagacttc ttttacttgt tgtgcttgtg gtcctttttt ctgtaagatc aaaatacttt
     1501 tttgcgattg tttattacgg aacaattcat ctggcaattg aatcattcct tgaagataac
     1561 cttcttcttt aaaccatttt ttgatttctt cactttgatc cgtttctaaa aatccactcg
     1621 gcatcaaaaa caaaccaaaa ccatccggct tcacatattt cattgattgc tctagcaata
     1681 aatgatgtgc ataactatgg ccttcttcgg tacttgttaa aaactctttt gctttttcgt
     1741 cattcggata ataaccgatt ggcaaatcgc tgattgcaaa atcgacagga tctattaata
     1801 attcctgcaa cccgtcttga tgaaagtatt gaacatttgc ttgtgtcaaa tcacttgtag
     1861 aagctgcgac agccaacaat gtatcatcaa tatccacacc aattccttct gtttggtacc
     1921 ccgcattcga taggtttaaa agaactgtta ataacaagtt gcccattcct gccgcaatat
     1981 ccagaatctt aaccggtgct tttttatttg tgtatagttg ttcaataaga aaaacaaata
     2041 aaaaaccgat actatcagga gtgagttgat ggttcgcctg caaatgttct gtctggctgc
     2101 ccttcaataa aagtaattga gacagtctgc gccattcttc tggctcgaaa gaaagtttct
     2161 tcaactcttc ataaagagct gtgatccgtt gcgttgtctc ttttgtcggc actccatcca
     2221 ccacacgtac ttgataatca tctattaaat tttctgcatt ttcgacatag gcttctaaaa
     2281 aggaagttcc caatgcattc tgtaaaagct gaatagcttc caggttttga ttaaaccctt
     2341 gttccatttt ttctggtaac atggtgcacc tcatttctag atatattata acaatttttt
     2401 cacctgttta attctatcga acaattgaca aaatttcaat cgttttcact gttttttatt
     2461 tgtgaaacgg caaacatttt tttattaaaa acagtgttat aacagaaaag agggcgtttt
     2521 cacgaacatt ccatcattct gtactagtat ctgtatcatt ttttacttct tctgtgaacc
     2581 gaaagataaa cggtgagact tccacctcta agaataggga ctgttgtacc cactgatatg
     2641 ttacagatcc agttgtgtag taaaaaactc cttccgaact ttcggcaagc gtagactgtt
     2701 ccgataaagc catctgcttc attatttttg ctatataata atttttgatt cgtatattaa
     2761 gatccattga caaacggtag ctttctaatg ctgccatcaa taaaaacgta cataaaagaa
     2821 ataaaagcaa gacagaaaat aaaatagagc ccttataaca ttttctactg atcgtttttt
     2881 gtccatatgg catatccatc ttctccattt tcaaagtgta cataaaaaga aattgtgtgt
     2941 tctttttcca ataattgaag ctcagtgata cttgttaaca taggttgatg tccgccggaa
     3001 gttcttcttt tacgtatcat tgcctgatat ctttcgatga tatactgttt atcctcgatc
     3061 gtgtagtgca gttgctgatt tttcacagag acattgtgac tgctcttgag ttcttcttct
     3121 aattgtgcta aaaatattag ccactctttt tgatctttac gctgcaaaaa ttcacttact
     3181 tggatggttt gctgaatcaa aagattgaat gtgagcaata gactgctcaa tatcatcagt
     3241 ccaattagac attcaatcag cgtaaatgct ggaattactt gttttttcaa tactgatcgc
     3301 ttctttctcc atattgacaa acactttttc gattactcca ttattatcga caatttggat
     3361 atcgtatggg atcatttctt cccttcgctc tggtcttttg tgatacatcc gctgcgtctt
     3421 gatatcttca taaatcattc gaataaatag cagttcctcc ccacttttct tttctgcctg
     3481 aagcaaaaac agctggccaa agctcactaa gtagacacag acagaagcga tcataagcgc
     3541 tgtaagggac tccaacaaaa caaatcctgt tttattcaat tctttttgta tagcgaccac
     3601 tccctatctg gaattgatac gtaatcgtcc gctctttttc ttcccaataa aattgataag
     3661 cttttagtga actttcattc ccggttctag cagcaaaagt aatagaagca tggcgtttca
     3721 aggtaatctc atcaggaata tctaatactt cccaatttat tttatctgga ttcggaacat
     3781 gaaaaatgat ttgattctct tcactattcc aataaaagcc tgtttggatc tgattcacaa
     3841 tagcgatttt ttgcgtagaa tagattcgtt tttcaaattg gccgaaaaat tgatagactg
     3901 ccaattcatt cttccatgca gaaaatgcga gaactggaaa agaaaggatc gttgtaagta
     3961 ataaaatcag taagagtgat tcaaacaacg tatatcctga tgctaaaacc ttaccttcaa
     4021 ggccgctgat agatttctgc atgttctcgt ttgatatagc cttcattcaa caattcctcc
     4081 acagtaggaa ctcttccatt attttgaagc gcatagagct ctttttgtgt gtctaccaac
     4141 tggatgatcg cttcacgact ttcttggtct acatgattcc tataacgcga caaattaggg
     4201 acgaacaaca acactaatac actgatgatc agtaagacga ttagcatttc gatcaatgta
     4261 aaaccgctat aataagatat ttttctcttt ttcatggcaa aacctcctct atgcttccgt
     4321 aaacaggtaa taaaacagcg gcataaacta ataaaatcaa agccgctacg cccaaaaaaa
     4381 tgattggctg aataaaatgc atccagtgat gaagacgttt gaaaagctgt tttcttgtcc
     4441 attcgctgta aaaaatcagt tctttaccta aacttccctt tgcctctccc tgtaaaacaa
     4501 ttttactgaa ttcttctgat agaaaacgat aattctggaa ctgctcagcc aatgacatac
     4561 ctgcctccaa tccttgaatc aacttagagg caagatgctg aatcagactt tcttgtttcg
     4621 tttcctttaa gcagactatg atttgcctta attcgaaacc ttcatgaaaa agctttccaa
     4681 tctctagtga caaataagcc gattgataca aggaatatat cggtccaatc aataattgtc
     4741 tgcttatcca ttcactctga tcaacaggag acaacttcat cagacgatac cttactagta
     4801 aagccaatag agtaaccgct aggatgaata gtagaacata ccaatgaaag acttgtatca
     4861 gctgaatacc ccaatgctct tgcatgacca ttcctgaacc aataagctga ggcaaaacga
     4921 acaaccgcat actaaacaag atacctagca aaaaggtgag caatagcatc ggatatgcaa
     4981 gcagcttttt caaatcattg cgaaatcctt caacaagccg aaactgtttg gcaatttcca
     5041 acaaagttgt cggcagattc ccatgacgtt cagctaactc gatctgtacc agttgctctg
     5101 ctccatatcc tatttgcgaa agtacctcac taaacgcttg cccttccttt agctgatgtt
     5161 ggatatttgc caaaataaca ggtggaaacg aacggatcgt gagtaataaa taaagggatt
     5221 cttgcagact aaaaccagat aaaagcagtt ctcccatggt ttgtgcaaac tggttttgct
     5281 ggcttcttgt taatccttta gaatagttct gactgccaaa tattttcctg agcttcttca
     5341 taactttcct cccatgattt ttttactgat tcttcctcca taaattgata actccataaa
     5401 acagaaggat ggtgattggc atcttccaaa agttcttgat aaatgacacc agctaaacat
     5461 tcagatagct cttctttttt ctttgtcagt tcatggattc tgaccaatgt accttcaaga
     5521 ttgcgtgcat gtacggttgc aaaaacccgg tgccctgtta gtgctgctct taccgcagca
     5581 tgtgcagttg attcatctct tatctcgcca atgattagca agtctggacg atgccgtaat
     5641 gcgagtctca aaagtgtatc ataagtctgt gctattttcg gattgatctg tagctgcaga
     5701 aaaaacggtt cttctatctc tacaggatct tcgatagtaa tcacttgcaa tttttcttcc
     5761 aaagccaacc gatacattag agaagtcttg cctgatccga ctggtccgct gaaaaggtat
     5821 aatccacgtc tgacggtttt ctgtaaaata gttgcaaaat ctcgagaact aaagcaacga
     5881 tggggtgttt caaaagtacc gtataatagc cgaatcacta aactctctcg ttgaaggtaa
     5941 tctcctacag aagacaaccg cagacgaact tcctgttttt ctaaagaata agtaaatgca
     6001 cctaactgag cttttctttt ttctccgata tccatctgtc ccaaatactt aaatcttgag
     6061 atcagttgct gtccatctgc cgaagagact ttcttccaga atgttcgttt ttttccatag
     6121 cgaaaataga tgtaaaatac ttctgaagct ggcaatatat atacgtcttg ggcatgtttg
     6181 gcaaaacttt cacttatcat ttgatccgat aatcgtttta tttccattat tctcccctcc
     6241 acaaaaaaca tacgtctttt tgatcttttt gtttcaaata aaatgaattt acaaaaaaat
     6301 attggcaaaa accgaattta tgttatactt ataaaaattt atcttgggag gcactccatt
     6361 ggaaacaaca ttaaggaaat atatcgaagt agcaagtaaa aaaaagcctg ctgacctagt
     6421 gatcaagcat gctaagatcg tcaacgtctt tacgaaagaa atcatggaaa aagatgtagc
     6481 aatctgcgaa ggaatgatcg taggaatcgg tgaatacgaa ggaaaaagcg tacataatgc
     6541 taatgggcaa tatctagtgc caggattcat cgatggccat gtgcatatcg aaagctcact
     6601 tttatcacca agcgaatttt caaaagtatc cttgcttcat ggagttacta ctgtagtgac
     6661 agatccccat gaaataggaa acattgctgg gagtacaggt cttgagttta tgatagaaga
     6721 tgcaagacaa acactcatga atatcttttt tatgctacct tcttgtgtac ctgtaacccc
     6781 ttttgagacc aatggtgcaa atttaaatgc aaaaaaatta gccccttttt tacaaaaacc
     6841 cgaagtttta ggcttagctg aagtcatgaa ctatcaagca gtagcaaata atgaaacaga
     6901 catcctagaa aagatccaat tgatgcacca gcataagaaa aaaattgacg ggcatgcagc
     6961 tggtatcggc atggaagagt taaatgttta tcctgcagca ggtattcgga cggatcatga
     7021 agctacaact gcaaaagaag ctaaagaacg tttggatctt ggtatgtatt taatggttcg
     7081 agaagggacc gttgcaaaag atctggcatc gctactaccg gcgatcacac ctgaaaattc
     7141 aagaagatgc ttctttgtca ccgatgataa actgatcaat gatttagtaa aagaaggatc
     7201 aatcgatcat atcgtccgtc aagctattgc acttggaatc gatccattac aagccattca
     7261 gatggcaaca ttgaatgcag cagaatgttt tgatttgaaa acactaggtg cgattgcccc
     7321 tgggtatcaa gctgatttct tcttaacaga cgatcttacc aagctgccaa ttcatgaagt
     7381 tttctataag ggaacattag ttgttcaatc tgggcaactc caagaccatt tatttgctga
     7441 tcattcaaat tcatatacgt gttgtctgcc aaaactaaac gctcaaccat taaatccaca
     7501 ttcgttagag ctgcttcttt cttcttcaca agcccatgtg atcgaaatta tcccgaatag
     7561 tctagtgact aaagatttgg tagaaaaagt ggaccgacaa ggcgaacgat tcattccttc
     7621 aatcgaaaaa gaccaattaa agattgcggt gatcgagcgt caccatcaaa cgggaaacat
     7681 tggattaggc atagtgaaag gatttcagct gaaacaagga gcgatagcga caacgattgc
     7741 ccatgactcg cataatctag ttgtcgttgg gacaaacgac gaggatatgc tgtatgctgc
     7801 taatcttttg attcaaaaag gtggcgggat gaccgttgtc aacaatcagc aggaaatagc
     7861 ttgtgtttct cttcctatag gcggaatcat gacacaggaa cctttttcga tcgtctatga
     7921 acagttgaat gaattaataa ctaaagcaca tcaactagga gcagctaaaa cctttgatcc
     7981 ttttttgaca ctttctttct taacattatc tgtcataccc gaattgaaga tcaccgacaa
     8041 aggattattt tcattttctc gcttccaatt gattccacct tgtggaaaaa ccgatgaaat
     8101 aaatacattt taaatcaaat aaatcccaga aaatacatta catctgtaat gttcattttc
     8161 tgggatttat ttatggatat taatgttttt atgaaagcag aaagttagtt tcacagactt
     8221 ttctttttgg tcttatcttg aaattgagct tctactcgat aaatacgttg accttttgaa
     8281 gaaaattttt cttcatactc cgtcataata ttgccttcaa attgactttg atgtaaatct
     8341 aaccatacct gtttgaggat catgccatat tgagaaaaac ttgccagaga atactcaaac
     8401 aatccttgat tatctgtttt gaaatggatt tctccatttg gacgaagaat ctgttcatct
     8461 acagcaagaa aagtcttata tgtcaatcgc cgtttttcat gacgtttttt tggccatgga
     8521 tcggaaaaat tcagatagat ttgatctact tcgctgtcag cgaaatactc tgtcaacgct
     8581 gatccatcca catgtagaag tttcaggtta ggaagcggtt gttcgatcaa cttgtctagt
     8641 gcaatcgaaa caacactgac ctgcatttct actccaatat aattgatttc aggatgggct
     8701 tttgccattc cagtgatgaa ctggcctttc cccataccaa tctcaatgtg gattggatga
     8761 tcgttttcaa acaattcatt ccattttccc ttccacaatg ttggatcact aattacaaaa
     8821 ttcgggtgag ccgctaacat ttccgctgct cccggacgat ttcggacacg cattatttat
     8881 ctccattcct ttcctctaat taaaaaggtc tggccaaatc tccgcagagt ttgggccaaa
     8941 cctcagccac tgaaaattcg ttttaattgt aaaatttctt cgttcatccg tcgatgatct
     9001 tcacgtttgc aatatctggt gatttcttgg agcaaattca tggccgcata ccaatgaaat
     9061 ttttcaagca tttcgtcagt cgggcgtatg ccataactga tcagccactg attccagcta
     9121 gacagtggca cataatttcc taaaatcgtt gcaatatcca ccgcagggtc tgcaaacatg
     9181 attgaatccc aatcgactag atataaataa tttttacata ctaaccaatt acgatggttt
     9241 acatctccgt gtactactga taagttttcc ttatctattg acgggatctg ctcacgaaga
     9301 aaacgatgga caatttgcag aaattgattt ttctggagtt cttccggtaa ttctttttca
     9361 tatttttgca gcatcttctc cggcgtcatg acacgaccac cgatacgggt cagcatggtt
     9421 ttaagagaat ttgaatgatg taaatggtac aaaacatcaa tcacgtcgtt tctttgacca
     9481 atctcattag gagtcagcaa gcgaccttcc agccactctt gtgctgtcaa agtatcgcca
     9541 ttccccgttc gttttgtcca aacaagtttt ggtgtaattc cttcccttga aagagcagcc
     9601 aacatcggag tcgtgtttcg tttaataaag actttgtcct catcacgcat gcctatgtat
     9661 gtttttcctg tgtcgccttt gatgggacgt aaccgccact cttggtctaa ctgaaactcc
     9721 atcatgcatt ctcccgtcct cttactcttt gttttgcagt acttattatt ctagtcgcct
     9781 gaacactaca cgtcaagcac atatacgttc tttttcattt aattgccatt caaaaaacca
     9841 gcttttctca aaaaaaatag aacgaacagc attcttactg tcaacgctct atttttcatt
     9901 ctcattttta ccctacacag ctttgttttc agtctgacat cttcttcaaa cgagttggta
     9961 catatagttt aacaaagagc atagtaacaa tcaaataagc cgctagcgga acccatcgct
    10021 gctgcccatc taatacgact gcagcaatca aaccaaaaat aaccgacgcg ataattaata
    10081 cccatgtgag tagatactga atcgcctgct tcttctgatt cttatggatt ggataaagct
    10141 gaacgagaac catatagcgg aactggttcg ctaaaggtaa cagttggaaa ccgatcaaat
    10201 ataaaaataa gaaaccgata atcagactaa aataaaagtc gactactccg gcaatcaata
    10261 acccacctat aattactaat cggaaataaa gaccactaaa atcagaacct cttgccaatc
    10321 ttcttgcaaa caagtaaaga taagtgtttt ttgattctct tttaatatga gtcaatatag
    10381 gatccaaata gcgtcttctt ttgatctttg ccgtaatttc aggaacatct gtgaataaat
    10441 tgataaattg atagatctga tgcaacctat gttcttctac atgcaccatt ttctcccaat
    10501 ccaatccttt ttcttgttgc ttccacaaaa accagtaata tagacttgcc tgtactaatg
    10561 cacctgctat tcctgcccat attagccaat aaaatgatag aaacaaaaag atcagagaag
    10621 aaaaaaacca gcctaatttc caataacgaa taacttgttg atctagtcga aacaattctg
    10681 ctctcttgat ttgcagatga ctgaatttca aactccataa tatgatcaga tagacgaaaa
    10741 acatcgaaaa ggattgctcc gtactaacca ccactaatgg catggccaat ccgcatgcca
    10801 atatcaaaaa gatcaacgga aaaatgcaag aatagcgaaa agctcgttcc aaataggttt
    10861 gcatttcttt ttctttagga agtaaaaaaa caatatctgc tgcctctgca aaagtagcca
    10921 tcttacccac gaataaacag agcatccaaa atacagagat tatgagccct ccaattgcaa
    10981 acggtggctg cagggttttc aaccaattag aataatataa tcccacaccg ccaatcaaga
    11041 aaagacaaac taaaacaaaa tgatcgttca atacataacg catatatttc atcattttct
    11101 tttgatgtct tgctaaacgt ttttgaaaaa aactactcat gtgcctgctc ctttgttaaa
    11161 gcaatataga tttcgtccaa agaagaatcc ggcagctgaa attcttttct caacatctca
    11221 agagaaccgt ctgctcgtat ctctccgtta tgtaagacca caaaacgatc acaatatttt
    11281 tctgcagtag ctaaaatatg cgtagacatc aaaataccgg ctccttcttt tttcatttct
    11341 accatcaatt caagcaatgc attgatagct agtggatcca agcctaaaaa tggttcgtcg
    11401 atgatatata aagaaggctc gatcaaaaag gcgcataaga tcatcacttt ctgtttcatt
    11461 ccctttgaaa aattagctgg gaaccagtca agacgattag ataaccgaaa agtctccaac
    11521 aacttttctg ctcttttcat tgctatatcc ataggtatat cgtaggccat cgcagtgact
    11581 tcgatatgtt ctctcaatgt caattcttca tataaagaag gagtctctgg aatataccca
    11641 atttgtttgc gatattcttc cgtatttttc tttaaggata gatgattgat ttctatcgtt
    11701 ccttttgaag cttccagcag accgataatg tgcttgatgg tcgtactttt ccctgctccg
    11761 ttcagtccaa tcaaccctac tatctctcca ggctgtacat cgaaactgat atcttttaac
    11821 acagggagat gtccgtaccc ccctgtgaca tgatcgataa ttaaactcat tctttttcct
    11881 ccgccattta tttttattta ttataccacg aagaaaatga cggtattttg tttttttcct
    11941 tacaatagct ttaacttatg gtaaagttaa gctgaagtat ttattcttta tagaaaggat
    12001 gttttatatg gaaaactgta ttttttgcaa aatcatcaat caagaaatcc caagctataa
    12061 aatatatgaa gacgacaagg tctatgcttt tttagacatc tctcaagcaa cgaaaggaca
    12121 cacattagtt gtgcctaaac gtcatgtagc agatattttt gaatatgatc ccgaacttgc
    12181 aggagaggtt ttttcgcgtg tgcctaagat tgctcaagca ttggaaaaag cttttccaga
    12241 aatgaaaggt cttaatatct tgaataacaa ccgtgaacta gcctatcaat ctgtttttca
    12301 ttcacatatc catttagtcc ctcgctacag caaagaggat gatttttcta ttcactttgt
    12361 caatcatcaa gatagttatg gatcggaaga actaaaagct attcaagaaa caatcgtaaa
    12421 gcaggtgtcc tgcgatgatt aaaaactttt tcaaaggact gttatttgga gctgctgctg
    12481 gcactgtcgg aggtctgtta ttggcgccca ggagcgggaa tgaaacaaga aagaaactga
    12541 tcgaagaatt ggaagaagca acaactctga caaatgattt aaataacagc ctgaatcatt
    12601 ttaaaagcgc attgatcgaa acaaaagaaa cagctgaatc tgtgattcct gctttccaag
    12661 aagcaatcaa aaaagatatc gaagatttta aattccaagc cgaaccacgt atcacacaaa
    12721 tcaatgaaca agtagaaaaa ctaaaagctg atctgccaga gtatcctgaa caaatataat
    12781 aatgattatt ttctcaacag atttaactat ataagtgaga aaagtttgcg tatttcctag
    12841 gaatgtgatg aaattttgaa ttttgtttga aactaccacg acaacgtttt taaaggaaat
    12901 gggatatggt atagttaata tataaaaata ataactaatt acaggagtgc aaattttaat
    12961 gaaaaaaaaa tcaattattt tggcagctac tagtgcgttg gctgttttaa ctctagcagc
    13021 ttgttcaggc gatactaata aagatatcgc aacgatgaaa ggcggaacga tcactgtatc
    13081 tgatttctat gatgaagcaa aacttgaatc atcgaaccaa tccttagtac aacggatgat
    13141 catttataaa gtattcaata acaaatatgg cgataaagta accgataagc aagtggatgc
    13201 agaatacgac aaacaagcga aatcattagg tgatactttt gaatcacaat tagaagctgc
    13261 tggatatacg aaagatactt ataaagaata tattcgtaac aatttagctt ttgaagctgg
    13321 cttgaaagct cacgtagata ttacagatga cgatttgaaa actgcttgga aatctttcca
    13381 tccagaagta gaagcacaaa tcatcaaatt atcaagtgaa gatgaagcaa aagatgtgaa
    13441 aaaatcagcg gatgatggag atgatttctc taaattagcc aaagacaaat caactgatac
    13501 agagacaaaa gaagatggcg gtaaagttaa attcgactca acgacaacaa ctattcctgc
    13561 agaagtaaaa gaagcagctt tcaaattgaa agacggtgag atctccgatg ttattactac
    13621 aacaaatcct acttcttatg cgacagaata ctacgttgtc aaaatggtga aaaaccaaaa
    13681 taaaggcaat gacatggata aatacaaaga tcaactaaaa gatatcgcta ctgaaactaa
    13741 actaagtgac aatgcattca caactaaagt aatcggtgaa gaattaaaag atgcaaatgt
    13801 aaaaatcaaa gatgatgcct ttgaaaatgt gttgagtgca ttcacaacaa cttcatcaag
    13861 tacgaaagac tcaagtgaaa ctacagcttc aacaaagagt tctgacacaa aatcaactga
    13921 ttcaacaaaa gaatcttcaa ctgaagaaac aactgattca tcaaaata
//
LOCUS       DAYYAG010000068        13917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000068 DAYYAG010000000
VERSION     DAYYAG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.068"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHARQ_001812"
     CDS             131..505
                     /locus_tag="ACHARQ_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8410220.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHARQ_001813"
     CDS             complement(619..1248)
                     /locus_tag="ACHARQ_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8410221.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHARQ_001814"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHARQ_001814"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHARQ_001815"
     tRNA            1668..1741
                     /locus_tag="ACHARQ_001815"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACHARQ_001816"
     CDS             complement(1879..2043)
                     /locus_tag="ACHARQ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410222.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACHARQ_001817"
     CDS             2338..3666
                     /locus_tag="ACHARQ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8410223.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACHARQ_001818"
     CDS             3868..4701
                     /locus_tag="ACHARQ_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8410224.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACHARQ_001819"
     CDS             4717..5454
                     /locus_tag="ACHARQ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8410225.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACHARQ_001820"
     CDS             complement(5609..6577)
                     /locus_tag="ACHARQ_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8410226.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACHARQ_001821"
     CDS             6809..7642
                     /locus_tag="ACHARQ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8410227.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACHARQ_001822"
     CDS             7676..8515
                     /locus_tag="ACHARQ_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8410228.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACHARQ_001823"
     CDS             8541..9101
                     /locus_tag="ACHARQ_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8410229.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACHARQ_001824"
     CDS             9300..11021
                     /locus_tag="ACHARQ_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8410230.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACHARQ_001825"
     CDS             11186..12328
                     /locus_tag="ACHARQ_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8410231.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACHARQ_001826"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACHARQ_001826"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8410232.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYAG010000069        13814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000069 DAYYAG010000000
VERSION     DAYYAG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.069"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..2132)
                     /gene="glyS"
                     /locus_tag="ACHARQ_001827"
     CDS             complement(57..2132)
                     /gene="glyS"
                     /locus_tag="ACHARQ_001827"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8410233.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2134..3051)
                     /gene="glyQ"
                     /locus_tag="ACHARQ_001828"
     CDS             complement(2134..3051)
                     /gene="glyQ"
                     /locus_tag="ACHARQ_001828"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8410234.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3435..4247)
                     /gene="recO"
                     /locus_tag="ACHARQ_001829"
     CDS             complement(3435..4247)
                     /gene="recO"
                     /locus_tag="ACHARQ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8410235.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4390..5289)
                     /gene="era"
                     /locus_tag="ACHARQ_001830"
     CDS             complement(4390..5289)
                     /gene="era"
                     /locus_tag="ACHARQ_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8410236.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5304..5699)
                     /locus_tag="ACHARQ_001831"
     CDS             complement(5304..5699)
                     /locus_tag="ACHARQ_001831"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8410237.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5683..6159)
                     /gene="ybeY"
                     /locus_tag="ACHARQ_001832"
     CDS             complement(5683..6159)
                     /gene="ybeY"
                     /locus_tag="ACHARQ_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8410238.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6176..8368)
                     /locus_tag="ACHARQ_001833"
     CDS             complement(6176..8368)
                     /locus_tag="ACHARQ_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8410239.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8392..9363)
                     /locus_tag="ACHARQ_001834"
     CDS             complement(8392..9363)
                     /locus_tag="ACHARQ_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8410240.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10069..11448
                     /gene="brnQ"
                     /locus_tag="ACHARQ_001835"
     CDS             10069..11448
                     /gene="brnQ"
                     /locus_tag="ACHARQ_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8410241.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11602..12048)
                     /locus_tag="ACHARQ_001836"
     CDS             complement(11602..12048)
                     /locus_tag="ACHARQ_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8410242.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12075..12251)
                     /gene="rpsU"
                     /locus_tag="ACHARQ_001837"
     CDS             complement(12075..12251)
                     /gene="rpsU"
                     /locus_tag="ACHARQ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8410243.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12413..12841)
                     /locus_tag="ACHARQ_001838"
     CDS             complement(12413..12841)
                     /locus_tag="ACHARQ_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8410244.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12933..13793
                     /locus_tag="ACHARQ_001839"
     CDS             12933..13793
                     /locus_tag="ACHARQ_001839"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8410245.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3777 a         2876 c         2495 g         4666 t
ORIGIN      
        1 aaccagagaa atagcctatt ttgtgcctgt tctctggttt atttacatat tagaaattat
       61 tttgtgatga tttgatctaa acttgctagt gacaaagcca tggcagcaat ttgacttaat
      121 tgcgttaatc ggttcttacg tactgctggt tcttctgcca tgaccatcgt ctgatcaaaa
      181 taattttcga tcagtgggcg aaggcttaca agctgatcat aattttcgtt gatcgtttgg
      241 tcagcaaagc ttgttttcat tgcctctact gctttgtgca gttctttctc cgcttcgttt
      301 tcgaacagtt cagggtctac ttctgattgt ttttcaagct ccgctttttt cgctaagttc
      361 acgacacgtg tcaaagcttc catcgatggt ttgaagtctt catctgcaag atgctgcttg
      421 aacaattgtg ctgctgcaaa tacttttgtc agatcttttt gttctgcatt caaaacagct
      481 tcgatgacat catgacggat attcttcgtt gtcaataatt ggcgcagacg gcctttgata
      541 aagtcgatca cctgttgttg tcccgctgac aagccaatgc caaaacgatc cgtatcttta
      601 ctgataactt cactaatcgt ttcttgcaga acagacaaag gaaaggccca ttttttcgat
      661 tcgatgattc gaacgacacc atatgcttga cggcgcaaag catatggatc gtttgagcca
      721 cttgggatca tcccaactgc aaagaaagaa aagatactat ctaatttatc agcaatcgct
      781 aaaacagaac ctactgtagt ctcaggtaaa tctccttcac tggatatagg catgtaatgt
      841 tcacggatcg ctgttgctac ggctggtttc tcgcctttaa gcagagcata tttttcgccc
      901 atgatccctt gtagttcagg gaattctcct accatgttgg ttactagatc aaatttatag
      961 atttcagcag cgcgttgcag atccatcaat tcttcctcag atagtcctac ttgttcgcct
     1021 atgatgcgag cgatgacttg tacccgttgc attttctcat agatgctacc gattttttca
     1081 tggaaagtaa cagtttttaa tttttcaacg tattggtcga ttgaagcttt cttgtcttca
     1141 ttataaaaga attccgcatc ttctaaacga gcagtcaata ctttttgatt gcctcgtacc
     1201 acattttcca attttacccg atcgccatta cgagcagaaa taaaatgagg aagcaacata
     1261 ccttgatcgt tccgtacttc gaaataacgt tgatgttctt tcatagaagt gactagtact
     1321 tcttcaggaa cagatagata tttttcttcg aagtcgccag aaaagacagt tggatattcg
     1381 actaaattgt tgacttcttc tagtaaatct tcatctagat caatgatcca gtgatttttt
     1441 tctgctaatt cgtttgcttg tgtgatgatc atttcttttc ttttttgcgg tgaagcaata
     1501 acaaattgtt cattcaattt ttcttcgtat tgttttgcat gcgtaaatgt cacatcttcg
     1561 cctaagaaac gatgacctct tgaagctctg tctgttttga catccaagat ctcaaacggg
     1621 attatttcgt catctaacaa tgccacgatc cagtgaatag ggcgaatata ttcaaaatcg
     1681 tattctgccc agtgcatcgt tactgggaaa gtgaggcttt taatgacatc tgctaaacct
     1741 gtcaatactt cttttgcttc tttgccgtga gtgtatttcg ttacatagac atattccacg
     1801 ccttttaatt ctctaaaagt gatagcatct gtcgtcagac cttgtccgcg gacaaatcct
     1861 tcagctgctt tcgtccagtt tccatctgcg tccaaagcaa tttttttggc tggtcctttg
     1921 acttcttctt cgctgtctgc ttgacgttct ggaatgtttg ttactttgat tgctaagcgg
     1981 cgcggtgtag aaaacgtttc gatcgtatca taagtcaatt ggttttcatc taaaaatttt
     2041 gctgtttttt cttctaattg tttcatgctt ggtgtgacta catgagcagg tatttcttcc
     2101 agcccgattt caagtaataa gtcttttgcc atcttattca gcctccttca cgttttctgg
     2161 atttgctgtg tttttattca ataaagggaa tcctaatttt tcgcgttctg ccacaaagat
     2221 tttggctaca gcacgagcca tgttgcggat acgggaaaga taacctgcac gctctgttac
     2281 ggatactgca ccgcgagcat ctaataaatt gaatgtatgg ctacatttca aaatataatc
     2341 atacgctgga tgaacaagat tttcatcgat acagcgttta gcttcttttt caaaacgatc
     2401 aaaattgctt agtagcaggt cttggtcgct tacttcaaaa gaatatttag aatgttcata
     2461 ttctggttgt ttgaagatct caccatattt cacgccatca gcccattcca gatcataaac
     2521 actttcaact tcttggatat aagaagcaag acgttctagt ccataagtga tctcagaagt
     2581 aactggttta caaggtagtc ctccgacttg ctggaagtat gtgaattgtg tgatctccat
     2641 cccatcaagc catacttccc agcccacacc ggcacaaccc atcgatgggt tctcccagtt
     2701 gtcttcaaca aagcgaatat cgtgttctaa tggatcaatt cctaaaagac gcaagctttc
     2761 taaataaagc tcttggatat tttcaggtga aggtttcatt acgacttgaa attgatgatg
     2821 ctgatacaaa cggtttgggt tttctccata acgcccgtca gctggtcggc gggatggctc
     2881 tacataagca gcattccatg gttccggtcc gattgcccgt aagaatgtat atgggctcat
     2941 cgtacctgct cctttttctg tatcataagc ttgcatcagc atgcagccgt ttgatgacca
     3001 aaatttttgt aatgttaaaa tcatatcttg gacggtcaat tttttactca tctgttttgc
     3061 ccctttcaat ttttaagcgt tgctgtcaat aagaatacag gaaagaaaac gacaaaaagt
     3121 ccctatgtct gattacagac atagggacga ataatttcgc ggttccaccc tacttccgaa
     3181 taaagattcg gcagcttcat ttatcgtact cgcaagtgcc tcttcaaaaa tcgtttgggt
     3241 ccggcttcca ctctcccgaa ctcgctaaac cacgtgtttt ttactgatct tggtcaatgt
     3301 acgtattcat tttgcagttt tctggtttgt ataatagatc ctctcagtga acgatctatt
     3361 taatctacgg ttaataatag acggaagtcc ctgtatttgt caaatagttt tgattttttt
     3421 gttgtatctt tcatttattc ttctttttca ggtatctctt tttcaggcat atttttctct
     3481 ggcgtcttta atacgtgtgc ccaagacttc atttgatcaa tgaacttttt gctttttaga
     3541 tgaataccga catattcatc atacaactgg tcaatgaggt cacggattgc ctgcttcgtt
     3601 tcttctttca agtggattcc actgatctta tcataagaaa tagccgaaaa cattcgaacg
     3661 aaataaacag cgcgaggatc tgcatgataa cggtgttcgt ccattggcca gtgtttctcg
     3721 catatcacac cgccatattt ggaagaatag tcaaattttc cttttgtttc cccgcatacg
     3781 acacagtgcg tccaaattgg cgcgattccg aagcgttgca gtatttgcac ttcaaagata
     3841 ttcgtgatga tttctgcatc atctccttgg tccatccgct gcagagcttg ttgcgtaaat
     3901 tggaataaat gcgggtcata ttgatgatct tcgattgctg catctactag atttagcaaa
     3961 tacgtaccat aaccgctcaa aaagatatct tcttggatgt tccgaaacgc atggacgtct
     4021 ttgacactgc ttaaaaacga caaaccttca cttcgaaaat taccgatata gactgcttcg
     4081 gtaaagggga gcaaagccgg agctaatggc tgattttgcc gatggattcc tttgatataa
     4141 aacatcattt tgccagaaga ttccgtaaag attttgacta atttatcttt ttctttgtaa
     4201 ttacggctag ataaaatcaa gcctttcgat tctgcaagct ggctcatatt ctccctccta
     4261 aaaaataaat ctggaaaagc agattctgcc gatccagatt atttatgttt cgatcttccg
     4321 ctgtttttct caacgtacgg atttttatcc agtttatttc tatacctaac ctataagaag
     4381 tccagaatat cagtactcgt cttttcggta gccgaagtct tgcagataga cttttttgtc
     4441 tcgccagtct ttttggactt tgacccaaag ttctagaaat actttgtctc ctaaaagatt
     4501 ttcgatatcc tttcttgctt tcgttcctac ttgcttcagc atttttccgc ctttgccgat
     4561 aatgattcct ttttggctat ctctttcgac gatgatcgtg gcctggatat ggattttatc
     4621 attctcgttt cgcttcatcg aatcaacaac gactgcgaca gaatgaggca cttcgtctcg
     4681 tgttaaaaac aagactttct cacggatcaa ttcagaaacg atgaagtatt caggatgatc
     4741 agtcacttga tcatctggaa aatattgtgg gccctctggc atttcactga ctaatacatc
     4801 cattaagcgt tcgacattgt ttccttctgt agcagagatc ggaatcactt gtgcaaaagg
     4861 catctgcgtc gaataatctt cgatgattcc cagcaactcg tctggatgga tcttatcgat
     4921 tttattgata atcagataaa caggagaatg cacgtttttt agacgttcaa taataaaatc
     4981 gtctccccgg ccacgttttt gatcagcact gatcataaat aagactgcat ccacttcttt
     5041 caacgcactg taagctgctt ctaccataaa atctcctaga cgatgctttg gtttatggat
     5101 tcctggtgta tcgataaaaa tcagctgagc ttctggtgta gtatatactc cttggatttt
     5161 atttctagtt gtctgtgcct tgtcactcat aatggcaatt ttttgaccca cgattcggtt
     5221 caataatgtg gattttccta cattcgggcg gccgacgatt gcgacaaacc ctgatttatg
     5281 ttctggcata tacgttctcc tttttaatta aacaacaaat gatatatttt agggacaaat
     5341 aaaatcaacc caatgatgac agcaaaacaa gcggtcacta aaactgctcc agctgccata
     5401 tctttgattt ttttcccaat cggatgaaaa tgaaaatccg taaacatgtc cactacattt
     5461 tcaaaaacag tattgatgat ctctactatc caaacgagaa aaacagctaa aagcagccat
     5521 aaccactcta tccgatcaag ctggaaaatc acgcccataa ataaagcaag gataccgaac
     5581 gcaacatgtt tcctcatatt gcgttcttcc tcaaaaacgg ttttgactcc ttggacagca
     5641 aattccacag acatcaggaa atgtttgttt ttctctacgt ttttatcttt tgagtccata
     5701 ggcatccaag atctctttct gtaaaccaaa catttctttc tcatcttctg gtgtcatatg
     5761 gtcatacccg ttgatatgca agaatccatg taccgctaaa aagccaagtt cccgatcata
     5821 tgaatggccg taagcttctg cttgttcagc agcacgttca atagagatca tgatgtctcc
     5881 tagtacacga ggtaatggat tctctccatc ttcaaaaatc actgggattt cgtcttcgcc
     5941 ttcttcttcc agagcaaaac tgatgacgtc tgtcggtgca tcttttccac ggtaatcacg
     6001 attgattact tggattgccg cattgtccat gaaagtaaca gacatttctg tttcctctgg
     6061 cagctttaaa taagatgctg caaattgcag caagtcatcg atttctttca ttttctcttc
     6121 tgagacttgg tttgtttcat ccataaaggt aatatccatt atacatcgct tctttctatt
     6181 cttcttgttc ttcctttatc ttctcaactt ctgttttcat tttaggatat ttgatccgag
     6241 aatggaaggt gctacttaaa ccgctgatta atgatttttc cacttttcgg atttctttca
     6301 aggtcagtcc actatcgtct aattgaccat cggaaatacg ctcttcgata agattatgga
     6361 caaaactttc gattttttcg ctcgtcggat gatccattgc ccgtactgca gcctcacaac
     6421 tgtcagcaat attgacgatc cctgcttcac gactttgcgg ttttggtcca ggataacgga
     6481 attgctcttc tgttacttcc ggattgcgtt ctttcgcttt gatgtagaaa taacgcatta
     6541 gcgtggttcc atgatgctgt ttgcaaatat cgatcaccat ttgcggcata ttataatctt
     6601 ccaatatttt ggctccatct gtcacatgtc caaagataat atgtttactg tcttctggca
     6661 ataagaaatt gtgcgggttt tccgctcctg aaggcaagtt ttcaacgaaa aagctagcat
     6721 gtttgatctt gccgatatca tgataatagc aagctactcg agttagcaaa gaacgtccgc
     6781 cgatttctgc tacagcgtta gcacttagat tagctaccat catactatga tgataagtac
     6841 ctggcgcttc ttccagcaac tgtttcaata aaggatgatt tggattgctg agttcattta
     6901 atacaattac actgtcatca ttgaccatca attcaatata tgggtgtaga ccgatcgtca
     6961 gcaagaatga gaagagtgca ccagtgaacc cgcagacgag cattaaccaa gtcgttgcat
     7021 caccaaagct cattccttga tagacgatca aaatgacatc catcatcaca ggaaaaataa
     7081 tgacccacat ggcagcagat aatccttgtt ctgagatccg tttgcgtctg acaatcgttg
     7141 ccatgagccc agagaaaaca taagacatca agataatcgt tagaaagttc gtgccaatcg
     7201 aatcataaaa aatgaaaagt gcggatactg cttggaaaat accagccatg atccctgctc
     7261 ttcgattcaa gaaaaagctt aaaacaagtg gtgcaaaagc agcgggataa aacagcggga
     7321 tataagtagc ttgttccgtt tggaataatt ggaaaaattt catcagcact atgctgactg
     7381 acatagctgt cacatagaaa agcacgtatt ttgttctttc tcctgcattt tggaattgaa
     7441 gtgaattata aatcagtacg ccgatttgaa gcaaaacagc taaaaccatg gcaattagcg
     7501 gaaaaatcga agttgtttga ttagtcagtc ctagtaattc caattttttc atagctgaag
     7561 catcgatttg attaccttca cgaacaatga tttctccttg gaagatcatc actggttgta
     7621 cagcttctcg tgcggattgt ttcaattctt ctgtcttttt ctcattcaat acgtcattca
     7681 ctacgatacc ttgatccact aagtagcgaa tcatttgttg ttgtgctggt gtgacattca
     7741 aatattggat ttgctcttct gcttcttgtt taaaagcttc caattcactt tcacgaactt
     7801 gtttgctcat ctgttcgtca atgagcttca gactctcatc cctcacagta tgaatctcag
     7861 tttcagtcat ttcaaatatc gtcgaataaa aattgttcgg tagtttttga taaaacacaa
     7921 cattttctgc attcacattt tcaaaatttt tcttcagcgc ggcgatccgc tcttctgtag
     7981 taggctttgg gatattttca tcttcctttg ccttatcaat cttttcgtca taagatttgt
     8041 tgatggcatc attcgtctta tcgatcaatt caaatagttg cttgatccga ttgttctggt
     8101 catctgcaag atctttttgg taagtgtatt ctggagtgac tgcttctgct gccagttttc
     8161 ttttttgttc ggtttcttcc gtgttctcga tcgttttgtt cgcacggata ctttcttctg
     8221 ctacttggcc ttctctgaaa tcattggcct tttgtttgac actactaaat gtaacgccgc
     8281 agataatgat aaaaaacaag aacagcagga aaggcaatgc tgcctttcca agtttttctt
     8341 gtagtttctg cagtgtttta ttcaacatgt gcttatcctc ctaagcaaag cttattcttg
     8401 atggagatct gccttttcat aagctttgat gatttcggca actacagggt gccgaacaac
     8461 gtcacttgct tcaaaattaa caaaatcgat tttcttgatt tgctttaatg ttcgttctgc
     8521 atgaacaaga ccacttgtcg tccctcgagg caagtctatc tgacttgtat ccccattgac
     8581 aatcattttt gattgataac cgagacgggt caagaacatt ttcatctgag cgactgtcgt
     8641 gttttgtgct tcgtccaaga taacgaatgc atcatccaat gttcgccccc gcatgtaagc
     8701 taaaggtgcg atttcgatta cgcctcgttc catcaatcgg ttcgtatgat ccaatccaaa
     8761 aatttggtat aaagcatcat aaacaggccg cagataagga tccacttttt cttttagatc
     8821 cccgggaaga aagcctagat tttcacctgc ttccacagca ggccgcgtca aaatgatttt
     8881 ttgcacttca ccttttttca aggcagcgat tgctaatact actgcaagaa aagtttttcc
     8941 tgtaccggct ggtcctacgc caaatacgat atcatgtttg gctaccgatt cgacatattt
     9001 cttttggccg ctgtttttta cccgtatagg ttttccatta cggtctttga cgatctcttc
     9061 ctcatacatc tcaacaaaat aatctaatgt acctttttgc cccattttca atgccgtcac
     9121 aacatcgggc gtacttacgt ggattcctcg tttgatcaat tcctgtaatg tccgaatgac
     9181 tgaggcagcc aaggaaatct gggattgctc accaatgatc tgaatgattt ctccacgtgt
     9241 atggatggtt gtttccgttg tttcctcaat cagttttata tgcttgtcgt gtgcacctaa
     9301 aagcatgctg acatcatcag cagccgtcaa tctaatctct aaagaactag actcttccgt
     9361 caaaaataga catccccttt ttttcgattc attctttcct attttaccat actcagaaga
     9421 aactatatag caaataagaa aaggttctaa aagagttgct taaccataag taccaagaaa
     9481 cgttaaaaaa ctagccgctt ccattctcta aaggtctcgt ttgaataaaa aaagctgatc
     9541 atgtaagcta tgcataagat agaggacaag tattccgtct aacttagcaa gtattctctg
     9601 ggtatttttc ttgtttcaaa aaaagaaaac aaaatatttg aaatattctt gcatttcaaa
     9661 aaaaacaggt gtattataac gtcaacaaat aaatatacag caaagcgtcg aggaaaacag
     9721 taggctattc agtgatttat tcccagtgag tcatgtaagt gagaaatgac aataaatcga
     9781 atagacgaac atcatttccg agtcaatgtc gaaaacgtag atcattgtgg ctgcctccat
     9841 tatcaggcta gcggataact ttatcgaaaa gcgtccgtga atgaagaagc ttttttaagc
     9901 gaaaaaaggt ggtaccgttc tagaatctag agcccttttc ccaagtggaa aagggctttt
     9961 tttatatttt aaggtggtgg ttttcatgga atctgatatt agtggtattt ctagtttctg
    10021 caacgtttat tttgttactc aaaatgataa ttattggagg aaaaatttat ggaagaaaca
    10081 aaaacttatt catggaagca attattgatg gtcagttcac ttatcttcgg gatgttcttc
    10141 gggtcaggta atctgatctt tcctgtacac ttaggtcaaa tggctggatc aaactggatc
    10201 agcgctggtg taggatttgc catctcaggc gctcttttcc cattattagc tatcttagct
    10261 gtagtagtga caaaaagtga tggtctgtat gacttagcta aacctgtcgg aaaatggtat
    10321 gctgccttgt tcttgatcct tgttcattta acgatcggac cattctttgg tacaccgcgg
    10381 acagcagcaa ctgcttttga aatggctgct aaaccctttt tgcctgaaaa atatgattca
    10441 ttaggtatgt ttttattctc tgctcttttc tttttacttg cttatttcct tactgtacat
    10501 ccaacacgtt tggtcaaata tgtcggaaag tatttaaact ccttgtttct tgcattatta
    10561 gctatcattt ttgtcgtttc tttattccaa cccatgggga atctaaacca atctgctgca
    10621 gattcttatc agacgaacgc tttgacaaat ggcgttttag aaggatacaa cacggttgat
    10681 gcagtagctt tacttgcttt aagtgtcact tttgtccatg cagttcgtga tcttggtttt
    10741 aaagataaaa aagtcacaga attaacagca aaagctggta gcctgagcgt tgcgttagaa
    10801 gtcatgattt attttggtct ggtccttctc ggtgcgatga gtttgaataa gatgtcttta
    10861 tcagaaaatg gcggaactgc actttctcag atcactaatt tttatttagg taacttcggt
    10921 gctagcttct taggggtaat ggtcacatta ggtgtcttca ctactgctat gggactcgtt
    10981 gtttcttttg cacaagattt ccataaactg ttccctaaag tcagttatat gacatggctg
    11041 cggttaacga cttttgtctc ttttgtcgtt gcgaatgccg gattggataa tatcatccaa
    11101 tggtctttac cagtattgat gcttttgtat ccattgtcac ttgcgttaat cttactttcc
    11161 ttaacagcaa aattcttcca aaagagtcca tttgtctatc aagtaacgat gttcttttct
    11221 gccgttccag ccgtattgga tatgttggca aattcgcctg cattagtgag tcagcaaact
    11281 gtcgtagcaa gcttactagg attttatcat cattatgttc cttttgctgc attaggtctt
    11341 ggctgggtcg tcccaacgtt gcttggttac gctggcagct tgctctttta ttatgtatat
    11401 cgcgtatcag gatataaaca agaagcaagt gagttaccag aagaataacc aaatcaaaca
    11461 caatttaaca taagagatat taaacaggaa aaaaacagct agaagaaaga ataagattct
    11521 ttcttctagc tgttttttag ttcttttttt acgaacacaa aaaactgcgg ataatcactg
    11581 tccgcagttt aaatgataaa attaagattg taatagttct ttcacaatcg cattcacttg
    11641 gtttccatct gcttttcctt tgactttagg cataattgca cccataactt tgccaaattc
    11701 tttcattgat gaagcaccag tttgagtgat tgcttcttga acaagttgtc gaatttcttc
    11761 gtcggaaagt tgttccggca tatatttttc gactattaca atttcacttt ttactttttc
    11821 agctaaatcg tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat
    11881 ttcacgagac aacaccgtta gttcttcttc ttctgtcaag tcgtgacctt ctttaatctg
    11941 ctcgttttgt atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc
    12001 tttcatcgct gctttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc
    12061 aattcatcac agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt
    12121 cttacacttg gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt
    12181 gacacggaac gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact
    12241 gtttttgaca tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa
    12301 atatatctca aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc
    12361 aaggaaatct tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac
    12421 aattttcaca tcttccgaaa atctcgaatc tatgcccttc gatcgtgcat ccacctagct
    12481 gttcttcgaa ataattcatc ggacacatat ggatttcttt cgttgttccg cacactgtac
    12541 agataaaatg atggtgatgg ccgattttcc cctgacagca atgaaaacga aacttcattt
    12601 ctccatttaa atcggtttct tccagtaaat cgatctctgc aaaatcatgg agattccggt
    12661 aaatcgtatc ataacttacc cctttgaatt cctgattcat atagtcaaaa acttctttag
    12721 cagatacgta gcggttacgc ttcactaaat aagttaacaa tgcttctctt tttttagtat
    12781 atttaaaacc gttatttttc aatgtttcta atgattgttc gatcaatgtc tgttttttca
    12841 tgacttattc ctccaaatca aaatgattac gaagtacgca ttctagagta tggtataata
    12901 aacataaaaa aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta
    12961 ctttttttgt catctccgac tctgctggag aaactgcgac aaaactagcg caagctacta
    13021 tggctcagta tcctaccgtt gagttcaacc tattccgcag aacgttcgtg acagatgaaa
    13081 aaacattaag gcaggcattg caagatgcat tagaagaaaa agcacttgtc ctgcatacat
    13141 taatcaatca agaactgatc gagataacac gttctttttg tgaagaacat caattattta
    13201 gttttgatgt gatgacgcca ccgatatcag aaattgaacg tttaactggt atcaagccaa
    13261 ttcggcaacc tggtgccctc catttattga atgagaacta tttcaaacgg atcaaagcaa
    13321 tggaatttgc tgtaaaatac gatgatggga aagatcctcg tggtttttta gaagcagatg
    13381 tcgttttgct aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca
    13441 aaaatttaaa agttgccaat ttacctttga tcccacaagc acatattccc aaacagcttt
    13501 gggagatcga tcctaaaaaa atcgtgggac taacgaataa cccagatatt ttaaataata
    13561 tccgtaaaga acggatgcgc tcttacggat taaaccctga tactgcttat tccgatatcg
    13621 aaaaaattag agctgaactt gattttgcga atgatcttta tgaaaaacta ggatgtgtag
    13681 tgatcaatgt ggcatcttta tctattgaag aaacggcttc gcttattttg aatgctttag
    13741 acctagaaga tcatagttat tatggaactg aaacaacaga aaaaagcgat taatcatttt
    13801 tgtctttttc ttaa
//
LOCUS       DAYYAG010000070        13719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000070 DAYYAG010000000
VERSION     DAYYAG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.070"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..362
                     /gene="yaaA"
                     /locus_tag="ACHARQ_001840"
     CDS             120..362
                     /gene="yaaA"
                     /locus_tag="ACHARQ_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8410246.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            349..1473
                     /gene="recF"
                     /locus_tag="ACHARQ_001841"
     CDS             349..1473
                     /gene="recF"
                     /locus_tag="ACHARQ_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8410247.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1470..3416
                     /gene="gyrB"
                     /locus_tag="ACHARQ_001842"
     CDS             1470..3416
                     /gene="gyrB"
                     /locus_tag="ACHARQ_001842"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8410248.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3436..5907
                     /gene="gyrA"
                     /locus_tag="ACHARQ_001843"
     CDS             3436..5907
                     /gene="gyrA"
                     /locus_tag="ACHARQ_001843"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8410249.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6143..6442
                     /gene="rpsF"
                     /locus_tag="ACHARQ_001844"
     CDS             6143..6442
                     /gene="rpsF"
                     /locus_tag="ACHARQ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8410250.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6492..7004
                     /gene="ssb"
                     /locus_tag="ACHARQ_001845"
     CDS             6492..7004
                     /gene="ssb"
                     /locus_tag="ACHARQ_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8410251.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7030..7266
                     /gene="rpsR"
                     /locus_tag="ACHARQ_001846"
     CDS             7030..7266
                     /gene="rpsR"
                     /locus_tag="ACHARQ_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8410252.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7409..9382
                     /locus_tag="ACHARQ_001847"
     CDS             7409..9382
                     /locus_tag="ACHARQ_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8410253.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9388..9840
                     /gene="rplI"
                     /locus_tag="ACHARQ_001848"
     CDS             9388..9840
                     /gene="rplI"
                     /locus_tag="ACHARQ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8410254.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10114..11481
                     /gene="dnaB"
                     /locus_tag="ACHARQ_001849"
     CDS             10114..11481
                     /gene="dnaB"
                     /locus_tag="ACHARQ_001849"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8410255.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12261..13553
                     /locus_tag="ACHARQ_001850"
     CDS             12261..13553
                     /locus_tag="ACHARQ_001850"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8410256.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4579 a         2473 c         3016 g         3651 t
ORIGIN      
        1 aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata aatttgtttt
       61 attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag ggatgggcat
      121 tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg ttgaaagaag
      181 tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac actgtgttag
      241 tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg atggttgaag
      301 ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat gaggctgaat
      361 aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt ttccaagaac
      421 ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga aagtatctac
      481 gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat tggctggact
      541 gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct tccattagaa
      601 atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca gaaaaagtta
      661 agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct ttctttagta
      721 aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca gatcaatcca
      781 atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa ccagtacttg
      841 aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt gtccgaacaa
      901 ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa gcgattggag
      961 ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct ggaaatcgag
     1021 tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca agaacaattt
     1081 ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac gacagttggt
     1141 cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac ttatggatcg
     1201 caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga tttgattaaa
     1261 gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga gctggatgat
     1321 aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca
     1381 acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt ttatgttcaa
     1441 caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta gtagaacgcg
     1501 ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc gttcgtaaac
     1561 gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt gtttgggaaa
     1621 ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc catgtgatta
     1681 ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct gtagatatcc
     1741 aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat gctggaggaa
     1801 aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga tcttctgttg
     1861 taaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat gtgtactatc
     1921 aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa acggatcgcc
     1981 atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca actgagtttg
     2041 atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc gggatgcgta
     2101 tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat tatgacggcg
     2161 ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt ccagaacctg
     2221 tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa tatacagacg
     2281 gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa ggtggaacac
     2341 atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa
     2401 agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa ggattgacag
     2461 ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa acaaaattgg
     2521 gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc atgaaattct
     2581 tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta gcttccagag
     2641 ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct ttagagatta
     2701 gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt
     2761 tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat cgagagtttc
     2821 aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc atggacaaga
     2881 tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg tttggagaag
     2941 attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat gccgatgtcg
     3001 acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg cgtcctattg
     3061 ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa caagggaaga
     3121 atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt
     3181 cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga gaaatggatg
     3241 atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc cgcgtcagtg
     3301 tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat cgagtagaac
     3361 cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat atttaatcat
     3421 gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt caatctaacc
     3481 agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc acgtgcactt
     3541 cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg catgaacgaa
     3601 ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg agatgtcatg
     3661 ggtaaatacc atcctcatgg agacattgcg atctatgagt caatggtacg gatggcacag
     3721 ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc tgttgacggt
     3781 gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc tacagaaatg
     3841 ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga tacagaaaaa
     3901 gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac aacggggatc
     3961 gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt agcggctatc
     4021 gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt attacctgga
     4081 ccagattttc caacaggtgg tttggttatg gggaaatcag gaatccgaag agcgtatgaa
     4141 acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat gccaaatggt
     4201 aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa attgatcgaa
     4261 cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt gcgggacgaa
     4321 tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag cgcatccgtt
     4381 atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt caatatgttg
     4441 gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaatccttga aaactatatc
     4501 gaacatcaac gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa agcagaagca
     4561 agagcacaca tcttagaagg tttgcgtatt gctttggatc atatcgatga aatcattgcg
     4621 attatccgcg ggtctaaatc agatgacgag gcaaaagcaa caatgatcga acggttcgat
     4681 ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt gaccggtttg
     4741 gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga agatttaact
     4801 gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt agcagacttg
     4861 aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt cttaagtctg
     4921 gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa caatggttac
     4981 atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa
     5041 ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc tacccatgat
     5101 acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata cgaaattcca
     5161 gaatacgggc gtacagccaa aggaatccca atcatcaatt tactaggtat cgattcgagt
     5221 gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca ctatctcttc
     5281 tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc taacatccga
     5341 agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg
     5401 acagatggtc aatctaatat cattattgga acacataacg gctactctgt cactttcaaa
     5461 gaagaagcag tccgcgatat gggacgtaca gcttccgggg tcagagggat acgcctgcgt
     5521 gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt attagtgatc
     5581 acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa aggccgcgga
     5641 ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc tggattgacc
     5701 actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat tatccgtttc
     5761 aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt gatgaagatg
     5821 gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc agaacaaaca
     5881 gttgaagaac aaggatcaga agaataacca tcaagactaa gtgtgtaagc tagcaagaat
     5941 aaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa tttctcttaa
     6001 aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta taattagagc
     6061 ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta agagccgaaa
     6121 gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt acattattcg
     6181 tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa
     6241 agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga
     6301 aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc catcatcagc
     6361 aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca ttcgtcacat
     6421 gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg
     6481 agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa gatccagatt
     6541 tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg aatcgtaatt
     6601 ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat
     6661 cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt actggacgta
     6721 tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca gaagtagtag
     6781 ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg
     6841 gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac tctaatcaaa
     6901 catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac ccattcggct
     6961 caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat ttgataggag
     7021 ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt agactacatt
     7081 gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa aagatttatt
     7141 tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa aaaccaacgt
     7201 aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt cgttagcgaa
     7261 gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc aattcctttt
     7321 tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt atggtaaaat
     7381 caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt tatccccgaa
     7441 acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa ttttaccatt
     7501 tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc gctattggtt
     7561 gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg cttcgactgt
     7621 agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga ttgcttatca
     7681 gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca aagatcaaag
     7741 tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa aaaagaaaaa
     7801 ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa agggtgttgt
     7861 ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga acagtcagcc
     7921 ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat tagatgacaa
     7981 agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga tgaatgaata
     8041 ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac agatgatcga
     8101 tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag cagaagctga
     8161 aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa gaaccctcga
     8221 tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg gaggcgacca
     8281 agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg ggaaatctgc
     8341 taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat
     8401 ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata tggatgcttt
     8461 aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag cttacatcgt
     8521 tatcaatgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa tccataagca
     8581 tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga aaaaagaaga
     8641 ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc agggcctata
     8701 tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag agtttccaag
     8761 taaacctctg ctgacttata ttgaatcttc agcttcttca gcttcagagc tagtttctga
     8821 gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg ccacattatt
     8881 gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg cacgaacttt
     8941 tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac aatatttatt
     9001 gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa gcgactatgt
     9061 gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata gtgttatagc
     9121 agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt tcgtcattac
     9181 aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga tcaacgtcca
     9241 aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta ctcaactgga
     9301 aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag aaaatatgaa
     9361 tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga tgtaaaagga
     9421 aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc
     9481 aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc aggacaaaag
     9541 aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc
     9601 ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga cagccgtttg
     9661 tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata caaaatcaaa
     9721 ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta cacaaacgta
     9781 cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt acaagaataa
     9841 caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa gacaaaccga
     9901 aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt
     9961 gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt ataataaaag
    10021 agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca ttttcttgta
    10081 aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca ggatcgaatc
    10141 ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct tgacgcagaa
    10201 acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg tggccatcaa
    10261 attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga cgtggttact
    10321 gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt aagttatctc
    10381 tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc gaaaatcgtt
    10441 gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt tacgaaagga
    10501 ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag tatcttggag
    10561 gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact gaactccacg
    10621 attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct accaactggc
    10681 tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat cattcttgct
    10741 gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca
    10801 aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc tcttgtaaat
    10861 cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg tcagctttcg
    10921 gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc aaatattttt
    10981 atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg
    11041 caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat tgaaggtaac
    11101 ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa aaaactggcg
    11161 aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt agaacagcga
    11221 caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga acaagatgcg
    11281 gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg agatgacgat
    11341 gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc
    11401 ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt ttcgtcgctt
    11461 tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc gagagctctc
    11521 gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta aacagatctc
    11581 caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat
    11641 cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat tttgacgttt
    11701 gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt aaacggtgtt
    11761 ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag cagttttttg
    11821 aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc ttaaacggtg
    11881 ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga gaagctattt
    11941 ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg
    12001 tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa
    12061 ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac ctctttttta
    12121 ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat aaaaataaaa
    12181 atcattcgat tttatttgaa atatgtatct attattgata caatggttca ggattaaata
    12241 atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa tggggcgatg
    12301 aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt gcacggtacc
    12361 aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat aaactgcatt
    12421 tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac ggcgttgtag
    12481 tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt gtagaaacaa
    12541 gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc aaattggacc
    12601 aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa ggaatcggac
    12661 ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta ttagataaag
    12721 aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa
    12781 tgtttgattc agaaccactt gattttgacg atatttttga agaatattat gaatacggac
    12841 aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct ttggatgcag
    12901 gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac cagggaactt
    12961 atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga agcggtgtag
    13021 ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg
    13081 atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc gaagtaggac
    13141 gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta
    13201 tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg atcgatgtat
    13261 taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc gaactaatct
    13321 accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat gaagaattgc
    13381 caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca gaaaacgcac
    13441 gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg
    13501 gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc taaggataaa
    13561 aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat ctatacgata
    13621 ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata ggctgtctct
    13681 tgaacactgt ttattcagaa taagggactg tgacaaaaa
//
LOCUS       DAYYAG010000071        13581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000071 DAYYAG010000000
VERSION     DAYYAG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.071"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..229
                     /locus_tag="ACHARQ_001851"
     CDS             <1..229
                     /locus_tag="ACHARQ_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8410257.1"
                     /translation="DAQNTVNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNL
                     NNYIEKLEKAKSDVEDTANRSQEIVTEHTGK"
     gene            complement(511..583)
                     /locus_tag="ACHARQ_001852"
     tRNA            complement(511..583)
                     /locus_tag="ACHARQ_001852"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(548..550),aa:Lys,seq:ctt)
     gene            964..1533
                     /locus_tag="ACHARQ_001853"
     CDS             964..1533
                     /locus_tag="ACHARQ_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8410258.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1520..2380
                     /locus_tag="ACHARQ_001854"
     CDS             1520..2380
                     /locus_tag="ACHARQ_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8410259.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2570..3274
                     /gene="yycF"
                     /locus_tag="ACHARQ_001855"
     CDS             2570..3274
                     /gene="yycF"
                     /locus_tag="ACHARQ_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8410260.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3279..5114
                     /gene="walK"
                     /locus_tag="ACHARQ_001856"
     CDS             3279..5114
                     /gene="walK"
                     /locus_tag="ACHARQ_001856"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8410261.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5111..6427
                     /gene="yycH"
                     /locus_tag="ACHARQ_001857"
     CDS             5111..6427
                     /gene="yycH"
                     /locus_tag="ACHARQ_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8410262.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6428..7297
                     /locus_tag="ACHARQ_001858"
     CDS             6428..7297
                     /locus_tag="ACHARQ_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8410263.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7352..8161
                     /locus_tag="ACHARQ_001859"
     CDS             7352..8161
                     /locus_tag="ACHARQ_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8410264.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8264..8893)
                     /locus_tag="ACHARQ_001860"
     CDS             complement(8264..8893)
                     /locus_tag="ACHARQ_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8410265.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9063..10355
                     /locus_tag="ACHARQ_001861"
     CDS             9063..10355
                     /locus_tag="ACHARQ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8410266.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10431..11093)
                     /locus_tag="ACHARQ_001862"
     CDS             complement(10431..11093)
                     /locus_tag="ACHARQ_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8410267.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11318..11602
                     /gene="groES"
                     /locus_tag="ACHARQ_001863"
     CDS             11318..11602
                     /gene="groES"
                     /locus_tag="ACHARQ_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8410268.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11653..13278
                     /gene="groL"
                     /locus_tag="ACHARQ_001864"
     CDS             11653..13278
                     /gene="groL"
                     /locus_tag="ACHARQ_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8410269.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4563 a         2326 c         2894 g         3798 t
ORIGIN      
        1 tgatgctcaa aatactgtta atagtttaaa tcaacaaatt aatcaattgt caaatgaaaa
       61 aaatactatt aaaaaccaat tgcaaagtac tgaacaaaaa ctagaaaaag cgcaatcaga
      121 aaacagtaat ttgaataatt atattgaaaa actggaaaaa gccaaaagcg atgtagaaga
      181 cacagcaaac agatcacaag aaatcgtgac tgaacataca ggaaaataga tgcatttttt
      241 agtttaaatt tctttaacaa aatagtattt tgttgctaaa taatttttat ttgaatattc
      301 tacaatgata tctcccctca aaatggaggg gaagtacata ctgtgataat ctacaggttt
      361 attgactgat taggatgtta ttaaaaaata aaatactaaa atgcataccc gaatttcata
      421 cccgaaaaat aaaaaatgcc accacgtaca ctcttttcga aactacctaa gtagccaaga
      481 atgtactggt gacgtgggtt tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc
      541 gctgattaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa
      601 taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc
      661 tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc
      721 ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact
      781 cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt
      841 agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac
      901 tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt
      961 atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt
     1021 ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta
     1081 gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct
     1141 atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac
     1201 aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct
     1261 gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat
     1321 ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa
     1381 tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca
     1441 actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat
     1501 ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac
     1561 ggatgtgcca gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat
     1621 ttataaagat caggtatata cagataaaat tgacattacc tctgaagaga tctatcaacg
     1681 tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt
     1741 tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt
     1801 gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata
     1861 tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa
     1921 atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga
     1981 aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat
     2041 cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa
     2101 tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa
     2161 aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc
     2221 ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc
     2281 acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc
     2341 gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg
     2401 aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt
     2461 tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa
     2521 tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt
     2581 tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga
     2641 aggctatgat gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt
     2701 agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc
     2761 tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga
     2821 aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc
     2881 caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa
     2941 ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga
     3001 agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact
     3061 actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac
     3121 ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt
     3181 gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt
     3241 cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt
     3301 ttcgatcagt caattttaag atcgctatta cctttattct gattttattg atctcgatcg
     3361 aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta
     3421 aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa
     3481 aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg
     3541 cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa
     3601 ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt
     3661 tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc
     3721 aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata
     3781 tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa
     3841 ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga
     3901 tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca
     3961 aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac
     4021 gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat
     4081 cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg
     4141 atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga
     4201 cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa
     4261 tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact
     4321 tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg
     4381 tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg
     4441 aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt
     4501 ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa
     4561 tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc
     4621 ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata
     4681 tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc
     4741 tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg
     4801 cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata
     4861 acgtggtttt aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag
     4921 tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac
     4981 ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga
     5041 gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg
     5101 aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc
     5161 gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa
     5221 cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt
     5281 ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga
     5341 aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa agctttctga
     5401 agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga
     5461 gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt
     5521 agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga
     5581 tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa
     5641 tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac
     5701 agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa
     5761 actgaaaaag tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt
     5821 tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg
     5881 tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct
     5941 gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt
     6001 aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg
     6061 gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga
     6121 agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat
     6181 tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca
     6241 gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac
     6301 atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa
     6361 atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc
     6421 gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa
     6481 tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc
     6541 taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa
     6601 gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac
     6661 ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt
     6721 agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga
     6781 tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt
     6841 cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat
     6901 ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct
     6961 ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc
     7021 tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac
     7081 attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta
     7141 ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat
     7201 cgaaacaagc gataaagtgg tccaaattga gagtgtcaac gctttcagca atacgatcgt
     7261 gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag
     7321 tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat
     7381 cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg
     7441 gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga
     7501 tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca
     7561 tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg
     7621 ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat
     7681 ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc
     7741 agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga
     7801 caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat
     7861 ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca
     7921 gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga
     7981 tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat
     8041 gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg
     8101 acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta
     8161 aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta
     8221 tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac
     8281 tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat
     8341 ctgccatgct gatcgtactg tcgccagcag cgtttctttg taaataatca tcacttaaca
     8401 aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc
     8461 aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg
     8521 gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta
     8581 agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct
     8641 caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag
     8701 catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt
     8761 gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta
     8821 aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga
     8881 gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat
     8941 attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat
     9001 cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag
     9061 aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta
     9121 gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct
     9181 cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa
     9241 caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt
     9301 ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag
     9361 tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat actcagatag
     9421 atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg
     9481 gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac
     9541 ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta
     9601 cgcttatcgg ttcagcggtt tttcaacagt tgttcagaat gactgttgtt caaggagtat
     9661 ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat
     9721 taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg
     9781 ggttggttag tcccgtcttg caagcgaatt cattagcgaa tgcagaaatc ttagcctcag
     9841 gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt
     9901 tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg
     9961 cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca
    10021 tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt
    10081 ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg
    10141 ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct
    10201 tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca
    10261 tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa
    10321 aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt
    10381 agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc
    10441 cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc
    10501 atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa
    10561 aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact
    10621 gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg
    10681 aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg
    10741 atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat
    10801 tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa
    10861 ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt
    10921 tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct
    10981 gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata
    11041 aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc
    11101 ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat
    11161 cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca
    11221 aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc
    11281 taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg
    11341 cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc
    11401 tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt
    11461 agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa
    11521 atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga
    11581 tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga
    11641 ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta
    11701 cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat
    11761 gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca
    11821 aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta
    11881 gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa
    11941 gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt
    12001 cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt
    12061 gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt
    12121 ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa
    12181 gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt
    12241 tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca
    12301 tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa
    12361 caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca
    12421 ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct
    12481 cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt
    12541 acagtaatta ctgacgatct aggtttagaa ttgaaagatg taacaatcga aaacttagga
    12601 aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa
    12661 aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct
    12721 gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt
    12781 gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat
    12841 gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct
    12901 ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt
    12961 atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt
    13021 tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat
    13081 gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta
    13141 acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca
    13201 gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg
    13261 atgggcggta tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag
    13321 acagataaaa agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg
    13381 gaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
    13441 ccgctgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct
    13501 cttttttgtg tgtaattata atgaaaatag ttatttttaa tagttataat attttatcta
    13561 ttaaaagtaa atatccattc a
//
LOCUS       DAYYAG010000072        13085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000072 DAYYAG010000000
VERSION     DAYYAG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.072"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHARQ_001865"
     CDS             complement(79..2199)
                     /locus_tag="ACHARQ_001865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8410270.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHARQ_001866"
     CDS             2429..3607
                     /locus_tag="ACHARQ_001866"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8410271.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHARQ_001867"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHARQ_001867"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8410272.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHARQ_001868"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHARQ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8410273.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHARQ_001869"
     CDS             complement(4960..6213)
                     /locus_tag="ACHARQ_001869"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8410274.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHARQ_001870"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHARQ_001870"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8410275.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHARQ_001871"
     CDS             7790..9136
                     /locus_tag="ACHARQ_001871"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8410276.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHARQ_001872"
     CDS             complement(9444..9830)
                     /locus_tag="ACHARQ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8410277.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHARQ_001873"
     CDS             complement(10098..10247)
                     /locus_tag="ACHARQ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410278.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHARQ_001874"
     CDS             10709..10921
                     /locus_tag="ACHARQ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410279.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHARQ_001875"
     CDS             complement(11199..12998)
                     /locus_tag="ACHARQ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8410280.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DAYYAG010000073        12943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000073 DAYYAG010000000
VERSION     DAYYAG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.073"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..855)
                     /locus_tag="ACHARQ_001876"
     CDS             complement(241..855)
                     /locus_tag="ACHARQ_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8410281.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1461..1838)
                     /locus_tag="ACHARQ_001877"
     CDS             complement(1461..1838)
                     /locus_tag="ACHARQ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410282.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2138..3019)
                     /gene="galU"
                     /locus_tag="ACHARQ_001878"
     CDS             complement(2138..3019)
                     /gene="galU"
                     /locus_tag="ACHARQ_001878"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8410283.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3062..4081)
                     /locus_tag="ACHARQ_001879"
     CDS             complement(3062..4081)
                     /locus_tag="ACHARQ_001879"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8410284.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4714..5718)
                     /locus_tag="ACHARQ_001880"
     CDS             complement(4714..5718)
                     /locus_tag="ACHARQ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8410285.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5731..6780)
                     /locus_tag="ACHARQ_001881"
     CDS             complement(5731..6780)
                     /locus_tag="ACHARQ_001881"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8410286.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6797..7618)
                     /locus_tag="ACHARQ_001882"
     CDS             complement(6797..7618)
                     /locus_tag="ACHARQ_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8410287.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7605..8384)
                     /locus_tag="ACHARQ_001883"
     CDS             complement(7605..8384)
                     /locus_tag="ACHARQ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8410288.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8401..8871)
                     /locus_tag="ACHARQ_001884"
     CDS             complement(8401..8871)
                     /locus_tag="ACHARQ_001884"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8410289.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8883..9302)
                     /locus_tag="ACHARQ_001885"
     CDS             complement(8883..9302)
                     /locus_tag="ACHARQ_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8410290.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9383..12160)
                     /locus_tag="ACHARQ_001886"
     CDS             complement(9383..12160)
                     /locus_tag="ACHARQ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8410291.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12351..12575
                     /locus_tag="ACHARQ_001887"
     CDS             12351..12575
                     /locus_tag="ACHARQ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410292.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3786 a         2711 c         2114 g         4332 t
ORIGIN      
        1 ttttattttt ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt
       61 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
      121 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
      181 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
      241 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
      301 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
      361 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
      421 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
      481 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
      541 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
      601 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
      661 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
      721 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
      781 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
      841 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
      901 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
      961 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
     1021 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
     1081 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
     1141 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
     1201 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
     1261 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
     1321 ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct
     1381 tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt
     1441 ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa
     1501 tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc
     1561 atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct
     1621 tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac
     1681 cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta
     1741 ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata
     1801 tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta
     1861 taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc
     1921 ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt
     1981 tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta
     2041 gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca
     2101 aatagccttt cattctcagc aggccactta tttccagtta actttctgtt cttagctttt
     2161 tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat
     2221 attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa
     2281 actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta
     2341 tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta
     2401 gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa
     2461 acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt
     2521 tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat
     2581 aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca
     2641 tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc
     2701 ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag
     2761 cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa
     2821 agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg
     2881 aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt
     2941 cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga
     3001 ctgctttttt gaccttcact tgctgttcct cctaaacgac gattaactat tgatcatcta
     3061 tttaacgaag agaaaattca ttctccattt ttccgtcacg taacatgatt tcttttgctg
     3121 cttttttgat atcttctcca ttatacaaaa cattatagat tgtttgagta attggcatct
     3181 caacacctag ctgttgagct agctctactg ctgcttttgt cgttgccaca ccctcaacga
     3241 ccattcccat gttttctaga acttcttcca gcttatgtcc ctgtcctaac aagttaccgg
     3301 cacgccaatt gcgagaatga acacttgtac aagtaacgat caagtccccc acaccactca
     3361 aaccgataaa tgtcagagga ttggcaccca tcgcgacacc aagacggctg atttctgcca
     3421 atcctcgagt catgattgct gcttttgcat catctccgaa tcctaatcca tggatagcgc
     3481 cggcaccaat tgcaatgata tttttcagtg ctgcaccagt ttctacgcca atcacatcat
     3541 tatttgtata aatcctgaaa taatcattca tgaacagttt ttgtacatag gctgctgctt
     3601 tttgatcaat acttgcagct gtaattgttg tgatatcatg tactgccact tcctcagcat
     3661 ggctaggacc agataataca acaattgcct gtcttttttc ttctggaatt tcttcagcca
     3721 aaacttctga gattcgttta tgcgtatctt gttctagtcc tttacttgca tgtataatga
     3781 ttggcttagt attcatttta ccaataagtt cctgtgcgac tgaacgaatc gcttttgttg
     3841 gaacaacaaa taaaatagca tctgcgttat ccactgcttc tgccaattct ttatatccga
     3901 taatcgattc tgggatcgtc aaatcaggta aaaaatgctg atttgtatga tacttattga
     3961 tttcatcaat ctgttcaggt acatttcccc aaatacggac atcatgcccg ttttctgcca
     4021 atgtttgtgc taaagcagtg ccccaagatc caggaccaag aactgcgatt ttttgtttca
     4081 tggatattcc tccaaataaa taattgataa tttccattat agtatacatt tcttaaagtt
     4141 gggactaatt tccattgttt ttaagtgttt gtttacttct tttatcgttt ttacttgaaa
     4201 taatcaatga tatgttacaa tagtctacat tttattttta cactcgttat gctttgcttg
     4261 ttcctaagag gaagcagttt tgacaatgtt actaccagca ttactattag gaggttagga
     4321 aacttctcct agtgagtatt gatttaaaat acataagaga cagattgaac atttctaaat
     4381 ctgtcaggtc ctgcaacgga cagattgtct ctgttgacgg ttgacatagg aacttttgtt
     4441 ttagtcaaca ttctggatac aaaacgctat gtaaactggc gcaactcgtt gtacttggac
     4501 ttttactacg gatccatgca acaactaaat agtactattg cataacaaaa aatggttggc
     4561 acccacatgt cccattaaac agattataag ttgaactgta gataatgcaa aaaatgtgtt
     4621 ttataacgta attgatgcca atattttaaa aaaataaagg gagcagttat cgctgctctc
     4681 tttatttttt atattctttt tatccagtct atcctaataa tcaaactgac gatagtagcg
     4741 gcgtatctct aatggatggc agttcaactg ctccacatga gcatcgatgc gttgtgtcac
     4801 tacatgcatc aataagaaag acaaaacacg tccgcgatat ttttcactga taccttctac
     4861 tggatagtct tttgtgtcta tcaatgtata attgctgcaa atttttggca aaagtttagc
     4921 cacacgttca gataacactc tctgttcatc ttcgcctaaa aataatgtga ctggggttgt
     4981 ttcttctacg atctctaacg tcccgtgaag aaactcggca gcatggatag atttcgatgg
     5041 aagccagctt tgttcttccc agtaacacat ggcatatgaa taaacagctc cccattgatt
     5101 ccctgctcca ataaaataat gcatactatc acgtcgatgt ttttctgcaa atgataagcc
     5161 aaatgcatct gcttttttct ctgcttccac taaccctgtt ggtaaataac gttcgagctg
     5221 ttcatagtat tctgaataag cttcgaattc tccattcaaa tacatcaatc gatctgctgc
     5281 cataaaaaat ttgatttgtt ctgttccagg agctggataa gtgaccacaa aatcgcatag
     5341 ctgagacaac ttactgttcg ctttatcgat aaatccaatc agtgtcgctc ctactttctt
     5401 gatttgttct actgctttca ctacttcttg agttgtaccg gtaacagagg acaaaataac
     5461 gatagaatct tttgttaatc ttttgtttcc agtagtgtaa tattctgcag catgctgtac
     5521 aaagacaggc aagttgcttt tcccattcat ataagtgaca gcttgcatcg aggaagcata
     5581 cgttccaccg attcctagat aatagacagc gtcaaatttt tccgcataaa gtttatcgat
     5641 gatttcttct acttgcggac gtaaagctag tgcgcctttt attgctttga tttgttcttc
     5701 ttcattgaat ttcagcatga tttccacctc ttataattta cttttcaaga ctgtatcaaa
     5761 gtcttcaaaa atctttttgt tcaagtcaat acctttagct actgcggttt tataagcgaa
     5821 tagttgaaac ggaacgacaa gcgctaatac agacagatat tcatcacatt ctattgctaa
     5881 tccaagtgtt ttctcgtttc ttgctttttt tgtggtaatg gtgagagtct ctccaacgta
     5941 ccgactcata tatctttgca acttttgcga acgttcctta tttacacttt ccccttcaat
     6001 aaagaatagc aaatgcgaag catctgcctc caggtatggg ccgtgcatat aagcttctaa
     6061 ctcaaatcct tgtgacggaa cgcggattgt ttcagtcaat ttcgtctctg cttctttagc
     6121 tgttccccag tttggaccat atccaatggt gacgaaacgc tgtgctaaac gaaataaact
     6181 ttgatatcca tcaaaaaatt cttccgtttt ttgaataatc gctggtaaat ggttgattat
     6241 tttgcgcaac tgttgcatgt agtcttgttc tatttttttg ctgattttat ttttacttat
     6301 tccaattcct atccctaaga gtagtaactg taaaacagtc gcagaatagc cttttgtaac
     6361 gaagcctact gattctaccc cggcacctaa atcaataatt tgtgcagctt ctaaagcaag
     6421 tggactttgc acgtcatttg tgatagcaac aacaggtaac ccttgttttt gtatcatttt
     6481 tactgcttca acagtagaag cacttttccc gctttgtgac acagcaatca cagtatcgat
     6541 tgaaggagaa agttttccat aattgaaaaa ataaaaaggt tcttgaatat cgacagtaag
     6601 atcaccataa gattccaatg ctggcttcgc tgctaaacaa gcgttataag atgaacccgt
     6661 tgctaagatc agtaaattca ctgttttttt catatgttct cttgtcgaaa aatcattctt
     6721 tttcaagatc gtacttagta cttccttttc ttcatagata taatcttgca tcgtaaacat
     6781 tttctctgct ccttttttaa aaaatcccta tccacgaacc aaaaattcca aagaaagcta
     6841 gtccgatcaa gatcgttaat ggtttcactt ctttcttcaa tagataaaac actatgccaa
     6901 atgctccaag acataagata tttggaacga tatcatcaaa aatactttga acagtcacag
     6961 cattttctcc tgaaccgatt tttaaaggaa tattgatggt gatcatcgtg gcagtcattg
     7021 ctccaacgac catcagtcca ataatagacg cgcctaatgt caaactttcc atcataccgt
     7081 ttgcctggat ctttttcaaa aaacccgttc ctaagcgata tccccaacct gttgctagat
     7141 agcgaaacag aatgtgcgga atattgaaaa tcaaaatgaa caagataggg cctaaaatat
     7201 ttccttgcat ggctaaagag gtacccacac ctgtggcaat caatcgcaaa gttccccaaa
     7261 agaaagaatc tcctaaccct gcaagaggtc ccatcaaaga tgttttgata ctatcgatcg
     7321 cagtcacatc aaattcagga tcatccttat tttcttcctc cattgcagcc gtgattccta
     7381 aaatcagtgt aacgatatgt ggggtcgtgt taaaaaactc tagatgccgt ttcaaagctg
     7441 ctgccatttc ttctttcgac tgatataact ttttcaaaat cggaatcatt gcataaacat
     7501 aagccatatt catctgacgt tcataattcc aagaaaactc catttgaaaa gaacgccaaa
     7561 atacgtgatt tagctctttt ttcgttattt tgctgttttt gatctcagaa ttcttcatct
     7621 tcttctacct cccctgcagt tgtttgaagt ggtagttgac ggacattcat aatattgact
     7681 accacaactg ctaaaatcgc accaaaaata gctactccgg tcacaggaat ctctaagtaa
     7741 gaagctatag caaaacctaa gaaaaaatat ggtgcaactt gtttgttgat cagcaaacga
     7801 gcaagcatcg caaaaccaag agctggaatc aaccctgtag ctactgacaa ccccttttga
     7861 acgaagtcag gaatcatatt taatagattt ccgatcgtat tgcttcccac catataagaa
     7921 accattacca caacaccaag catcaaggaa agaccgatac cggcaagcaa gtgcatatgc
     7981 tcgactcctt tataatttcc ttcttctgca taattgtctg ctttatggtt cataattgga
     8041 atcaaaagcc ctaagtaaat atttttcaat atcaatgtca acgtagcaat cggcaaacct
     8101 agtaaaagcg cggtttctgt accggctcca gctgtaatag caaaagccgt ccctaaaatc
     8161 ccaccggtca caacatcagg tggaatcgaa gcacctactg agaacgatcc gataaatgct
     8221 aactctaaag ttgcgcccat aatgatccca gtttttacat cacctaacac gattcctgta
     8281 aacagccccg tcacaatcgg acgagaaagc aacgatgtcc ctagtgcata ttctgattgt
     8341 gcgacaaatg caacaattcc taataagact gcttgtacaa tcattgaggt tcttcctttc
     8401 ttacaaaact tctcttacat ccactttttt atctgctggt acttgacgga tttctacttc
     8461 tatttgtttg tcaattaatc gtttcaagcg ttcctcctct tctggtaaaa tatttaccgc
     8521 ttttccgatg ctgcgagtac caggttttgc cttgatccct cctaagttga tggttttgat
     8581 ttctggatac gcttctgcca gcttatatgc atcctcgatc gattccacta cgatgaataa
     8641 cttatattta tctgtcaccc cgcttttcaa cgcggcaatc gagtcatcaa ttgatttgat
     8701 cactagtttg actccttgtg gcttggctaa tttgatcgtt gtctttcgta tctcattcgt
     8761 aggtacatca tcattagcaa tcaaaataca gtctgcgcct attgtttgtg tccatgaaaa
     8821 agctacttgt ccgtgtaata accgatggtc gactcttgtc aatacaatca tttttcttcc
     8881 tcctaaaatt cgtcttcttc tattttctga ttgagccgct cgttacaaaa ctggatcatt
     8941 ttttttgaat cagttagcac ctgctggatc aaaacagttg tctctacttc agcgtccaac
     9001 cgagatacta gctcaatcaa tagtggcaaa ttcaatccgg caacgagata gacatgatcc
     9061 cgattgatat attgtaagaa ttcgttgttg acacttccgc caaaaagatc cgtcagaaca
     9121 atgatttctt tatctttgta cctcataaaa agctgcctta ctgtttccgc taaatcaaaa
     9181 ttctcatcag tatagcaatc gaatacagta atagaatgat ttttcccata gatcagctct
     9241 aatgaatcta atatccctga agcaaatttt ccatgggaag ctaagataat ttctctatcc
     9301 actttttcat ctcctctttt cacttacaca tactaaaaaa gcaagttgca tgccaaaatt
     9361 agtggcaaca acttgcttat gttcaaaatt cttcttctat cgagagaaac tctgtatctt
     9421 cactcaaaat atcatagata taggcaatct cagaattggg gatcatgaca ctataatgct
     9481 cttcaatgac actaaacgca gatttaatag cagataaccc ttctttttga cactgcttcc
     9541 atttctctaa tccttgatag gtttcgattg gcatttgtcg gatcaatcgt tcaatgagac
     9601 agctcacatg gacatacaac gctaattttt tgggattgct gatgcttcga ttcaactgca
     9661 cctctaattc tctcataaaa tgatcgattt ctcccatcac cttctctgta tctagaatag
     9721 tcacggaatc cagtactttt tccaatgaaa aattacgaat gatgcgctga ttgaatattt
     9781 ccttctctga agcattcata gtagtgtcca accattcagc tagtacagac atcttttccc
     9841 ccgagatgag gtcttctaat gaaagatagt cgatattggt gatatcagga tctgtggtgc
     9901 caatcattcc tacgacttcg tatatggaaa ataatgggtc actgtttttc ttatctttta
     9961 attgctgata ttcatatggg atgattttca actgacaagc cgtaggtaag cttttttcta
    10021 gcagttcact taagtggacc gccgtaccga ttccagtgaa gcaagtagta atgagtgctt
    10081 tgggtttgtt ttcctctgga tagagcagtc gccattctgg ctggatctgt cgaacagtct
    10141 cttctgctat ctcttctaat gatacatttt tttgcaggca ttcccctact gtgattgcta
    10201 aaggtgtcgt cacattattc aaaatcaaaa gcggtacgga taactgtttt ttaaaaaact
    10261 gatggatttc tttcagagat cccatgtcta ctaatatgat caatccattt gaaacatcgc
    10321 tgttttcgca ataatcaagg atttctgaag caatctgctg tggtgtgaca tctagtggca
    10381 tatcaaatga ttcaaataga tcctcttgta ataaacgatt tactacattt gccatactgc
    10441 tagcagttgc ataaccgtga gcagcaatca tcgcttttgg cagtcgtttt tcttttgacc
    10501 atttcgcttt ttttagatag atcgtcagta aaatccgatc catacttgtg tcttcaatat
    10561 ctaatttgat cttacttaat tccagaattc gttggacata gtgataacta gatggataag
    10621 actgagaaac ttgttgttct aattctttga ttagttggat aatatcatgt tcttccggta
    10681 accatttgat agtgcttcgc tgatagagat agtaactcaa ggcatagaca ctgtttccat
    10741 cgaatttgat ttgataggca gtttccattt gtcgaaaggt ctctcggata gattgtgtcc
    10801 aataaaccag cagctcgtgt ttttcttgtc tactggtctc aaacagcaaa aaatcaaata
    10861 attgaatcac ttcttgcttt aattttcctt cgcaagaata tagcagacag ttatttttct
    10921 tgaaatcaat taggatttgc tcaaaacttt taacgattcg ctgctgttcg ggctgttgct
    10981 tctccattaa ttgctctaag gtctggtctt gcgtaatata gattccttgc tgtgctgtat
    11041 ttttagtaat aggctgcgca agtagttctt tttgcaaatg atggatcgtg acatggattt
    11101 caggctgttc acgctgttca gcatatgctt ttgctgctga aactttgacg atatttttca
    11161 gctctcctat gtttccggat aatttctgac ttgttaaaag tgacaaagct ggtccactga
    11221 ctgacaaagg gctccccatt tttctttgtt cttcgagaaa aaaagagtaa atcagttcta
    11281 gccgttcatt acgactacgc tgattaagtg caggtagttc gattcggata ggtattcttc
    11341 gaatgaaagt tgttaagaaa tgactagtaa tctcttcagt cgtagcaaaa aagagacgtg
    11401 ttttgatccg gcgtgacaaa ttaggttctc ccatacgata aatcacacct tgatcaagat
    11461 aggtgaacag tttttcctga ccttctggtg ttaagcgatg tacctcgtct aaaaacaaga
    11521 tcccaccatc tgctgcttca aaagcccctt ttttattttc ttctgcacca gtgtacgctc
    11581 ccttaacatg tccaaacaaa ttgctagtca aaagttcagg attatctgca tattgtgcac
    11641 aattaaaagt gatgaatggt gcagaatctt ctagcagatc atgaagcaga caatactgat
    11701 agaccaaatt aactaaataa cttttacctg tgccactttc gcctgtaatc aaaagtggta
    11761 atccgccatc tggatagtaa agagctgttt tgatttgttc gatactttct ttcaaactgg
    11821 cgtcgtatcc gatcataaaa gagaataagt cttttggttg cgcgaaaaaa ggctgttcct
    11881 ctttgagatc atcgatagaa aaataaaaat tcgtgcgtaa cttaaaaaat tgtttttcaa
    11941 aagcttcttt gtggaaataa atcactggtc gagaattgat tttgactagt agttgctcat
    12001 catttaactg gttcaaataa tgactgactg tgtttctttt taccccaaaa tgatcagcta
    12061 acctttgtgc tgtaaagacc tcgttcatat gttctaaatc gaaaaaggct gtctggttct
    12121 ctaagtaaca taaaatttcc ttcttcaaca acttattcac ccctcctcca tcatagaaaa
    12181 cggattcatt ttgtcaataa aatctttaat aaagaattga aaaatatatc ttattataga
    12241 cagcagccta cttatccctt ataattttta ttctaaaaaa cgtacagttc cttccagttt
    12301 ttatactgga atttcaacat atgaaagaat aaggaggcta tgactattca atgaaatcgg
    12361 ataaatataa aaacaggaaa catacttcct ggaaaacttt tcgagaaatc atcggatctt
    12421 atctcgctgt agttgtattt ttgcctttgc tttatatggc tatgacagaa caaactcgtt
    12481 ttattggcgt agatacgcaa tatatagccg aaacaggatt tttattcatc tttatcttga
    12541 tttttgcgtt ctataaatcc ttccatacaa aataagattc acagatttca actttcctgt
    12601 ctgtttttag ctattacaaa tccttatatg tggtataaca acaatttatt ggcataatga
    12661 ggaaaaacga gttatgatgc tttcagagag taaagaggta ctgttgtcac catccttttt
    12721 attgattacg tacaccactc ttagtttttt cccattgatt acgaaaacaa ggtagaaaca
    12781 ttgatgttcc taccttgttt tcttttttta ttttcagatc aaaatctcaa gtggcttttg
    12841 acttgatgtc gggaggttgt cttcgttcca tcaaacctga tagaatctgc gccaaaccat
    12901 agttttctcc actattattt attcctctaa gatagtaccc aca
//
LOCUS       DAYYAG010000074        12843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000074 DAYYAG010000000
VERSION     DAYYAG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.074"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1372
                     /gene="efmA"
                     /locus_tag="ACHARQ_001888"
     CDS             86..1372
                     /gene="efmA"
                     /locus_tag="ACHARQ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8410293.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1510..2109)
                     /locus_tag="ACHARQ_001889"
     CDS             complement(1510..2109)
                     /locus_tag="ACHARQ_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8410294.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2513..3634
                     /gene="mnmA"
                     /locus_tag="ACHARQ_001890"
     CDS             2513..3634
                     /gene="mnmA"
                     /locus_tag="ACHARQ_001890"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8410295.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3880..5301
                     /gene="arcD"
                     /locus_tag="ACHARQ_001891"
     CDS             3880..5301
                     /gene="arcD"
                     /locus_tag="ACHARQ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8410296.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5315..6433
                     /locus_tag="ACHARQ_001892"
     CDS             5315..6433
                     /locus_tag="ACHARQ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8410297.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6493..7785)
                     /locus_tag="ACHARQ_001893"
     CDS             complement(6493..7785)
                     /locus_tag="ACHARQ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8410298.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7935..8495
                     /locus_tag="ACHARQ_001894"
     CDS             7935..8495
                     /locus_tag="ACHARQ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8410299.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8624..9001
                     /locus_tag="ACHARQ_001895"
     CDS             8624..9001
                     /locus_tag="ACHARQ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8410300.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8934..11006
                     /locus_tag="ACHARQ_001896"
     CDS             8934..11006
                     /locus_tag="ACHARQ_001896"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8410301.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11217..12164)
                     /locus_tag="ACHARQ_001897"
     CDS             complement(11217..12164)
                     /locus_tag="ACHARQ_001897"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8410302.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12410..>12843
                     /locus_tag="ACHARQ_001898"
     CDS             12410..>12843
                     /locus_tag="ACHARQ_001898"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8410303.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4139 a         2337 c         2739 g         3628 t
ORIGIN      
        1 aaaaaggtat tttttgaaaa aagaatcccg tataatgatt gttgtgcaaa cgctatacaa
       61 aatgaaaaag aaaagaggga tgtttatgga aaatgaacag tcggtcgttt taacgaattg
      121 gaagagaaat tatttgttct ttttatcggg acagtttttg tcaggaatca ctagtatggt
      181 cgtccaatat gcgatcatct ggtatttgac aagagaaacc ggttcggcga caattttgag
      241 ttttgctact ttactaggaa tgattcccat ggttttacta agcccatttg taggaccgct
      301 agtggatcgc tgggataaaa aagctctttt gattgtcacg gatattattg tagcgatttt
      361 cgcgcttatt ttagcagtag tcggaacgat ttcagaatcc ttcccgattt ggctagtttt
      421 tgtgtctttg tttatgcgtt cggtcgcaca aacattccaa atgccaacga tccagtcgat
      481 tatgccgaca atcgttccat cttctcacat aacaaggacg aatggacagt taggaatggt
      541 ccaatcagcg aatttcatca ttgcgccagc tctaggtgcg gctttgtttt cagtcgttcc
      601 tgtcaattac cttattttat tagatgtatt gggggcagtt tttggtgtcg gcttattgat
      661 ttttgtgaag atcccaaaag tttctcctga aatattggaa gttccgctta ctatttttaa
      721 agatgcaaaa tttgggctgc agcaattgat ggataataaa gggttatggt acataacgat
      781 caatggtgca tttgtgatgc tgttgttcat gccagcaatc agtctgtatc cattgatgac
      841 gctagattac tttggtggtt ccgttggaca ggcgggagca gtggaagtag tctacgcagt
      901 cggaatgctc ttaggcggtg cactgatcag ttttatagga acatggaaag accgaatgaa
      961 acctattatt atagcctaca tcattatggg cctgacgatc ggtgcaagcg gattggttcc
     1021 aaacgatagt caagggttct tgtactttct tatcttgaat gcaggagcag gttgtgcaac
     1081 gccatatttc aatacactgc tgatggcaat gatccagcag agttatgaat cgaatgtttt
     1141 aggtcgtgtc ttagggaact tcaactcttt gatgaacctg gcaggtccaa ttggattatt
     1201 atttgcagga ccattagctg atcgtttagg ggtagaaaaa atgttcttgt tttccggaat
     1261 cgggatttta ttatgcggaa tcgttttatt tttaacgtca gctgcccgaa attatgataa
     1321 agaattacag aaaaaactgg taaaagaaca ccatgagcaa aaagacgaat agtatggaat
     1381 ggagttgtga cagaagtctt gttacaactc catttctgat cctaatgttc ctgtggcaat
     1441 aagcctactc aaatgaaaaa tatgagtaat tatctttatt tgtttaatac ttgatatagg
     1501 gcagtttttt cacgacctat cgtacttcct ctttgcttca tagatttctt tgatttgttg
     1561 ctcagataac ggagtgccaa atggtagttt ttcctttaag agttctcttt ccggtgcgtc
     1621 tacgcctaca ttgacatcgt tctcgatagg tacgctgtaa tgatggatat gtccatgaag
     1681 attgcggata gtgtcgttag gcccgagcaa catcggataa tgagtcaagt aatattgttg
     1741 atggtcgaat ttaacgatgg cacctacatc ataaaaataa aatttgggac ttccgtcagt
     1801 aagcctagga tcattttttt ctaaataacg gaaaaaagcg cgactgtcat ggttcccttt
     1861 gatcaagtgg attgttcctt gcagttgagt gaccagttcc aaaatttctg catttgcttt
     1921 ttcatattgc ggatgcatag caaggtcgcc tagatgataa acatggtcgg tttcttttat
     1981 tactgcattc cagctagtga tcaactgttg gttcatttca tccacatttg caaagagccg
     2041 cggagcaaag tcgttttttc ctaataagcg ttcatggaaa aagtgcatat cactgataaa
     2101 gtaattcatg tatcttttgc ctcccttttt tcttagagta aacaaaatca cttatctgag
     2161 caactcataa catctaaaaa gaaaaggttg taaatgagac gtgcagcttt aagcgataag
     2221 aagcaatttt gaaaaatagt ttttcacaat ttataaaatt attccttatt gccgaaaggc
     2281 tggccctttt tatgagtatg ccaatttttt atatcgaatg ctcaaaagat gagtgatttt
     2341 ttggttgact atcatgtttg tgtactgaat aggtccagta gaacagtatt tttacgactt
     2401 gataattaag ggatgaaatt ttcagaaata tgacgtataa tgtaaaatac tgaaaagttg
     2461 cgaccttcaa ctcgtagatg tttcagaatc taattgaaat gatggtgata tcatgacaga
     2521 caacagcaaa acacgcgttg tcgtagggat gagcggcggt gtcgactcat ctgtaacagc
     2581 acttttgtta aaggaacaag gatacgacgt gatcggaata ttcatgaaaa attgggatga
     2641 cacggacgaa aacggtgtat gcacggccac ggaagattat aaggatgtag cgaaagtagc
     2701 tgctcaaatc gggattcctt attactctgt gaattttgaa aaagaatatt gggatcgtgt
     2761 atttgaatat ttcctagccg aataccgcgc aggacgtaca cctaacccag acgtgatgtg
     2821 caacaaagaa atcaaattca aagcattctt ggattatgca atggatcttg gcgccgatta
     2881 tgtagctact ggacattatg cgcaagtcac tcgcgatgaa aacggcgtgg tccacatgct
     2941 tcgtggagta gacaataata aagatcaaac ttacttcctt agccaattat cacaagaaca
     3001 gctttcaaaa acgatgttcc ctctaggcgg catggaaaaa tcagaagtac gggcaattgc
     3061 tgaacgcgca ggccttgcga ctgctaagaa aaaagattca acgggaatct gctttattgg
     3121 tgaaaagaac ttcaaacaat ttttgagcaa ctatctgcca gcaaaaaaag gaaatatggt
     3181 cactcttgat ggtgaagtca aaggacaaca tgctggcttg atgtattata cgattggtca
     3241 acgtcaagga ctagggatcg gtggcggagg agattcgcaa gaaccctggt ttgttgtagg
     3301 aaaagatcta gcgacaaaca cattatatgt ggggcaaggg ttccatcatc ctgcattgta
     3361 tgccacaagc ttggatgcaa gtgaaatcca ctttacgacg aatgaaccaa tgccaaaaga
     3421 attcaagtgt acagcgaaat tccgttatcg tcagcaagac gttcctgtga cagttcgctt
     3481 attggatgat aatcgtgcag aagtcgtatt cgacgaacct gtacgtgcaa tcactcctgg
     3541 acaagcagtt gtattttatg atggaatgga atgtctgggt ggtggtttga tcgaccacgc
     3601 atatcaagaa acaaaagttt tacaatacgt ttaagcgata ttaaatgaat caaaacagct
     3661 atggctaaca aaagaaacac cttttctttt ggatagcata gctgtttttt tgcaataaaa
     3721 ttaattttta ttcattttat acttgaaata cttgtgtaaa aagttcataa tatccaataa
     3781 tatgaataaa gttgcttttt gttaagcaag agcagttgca aaatagaagg gaatcttttg
     3841 caagtgattt gttcaatttt ataagtgagg gtgatggaaa tggaaaagaa atcgacgggt
     3901 attagcaaat ggggactggt tgctctggtt gtcagttcgt caatcggaag cggtgtgttt
     3961 gggatcacaa gtgatctggc tagttctgca gctccagggc cagcgatcct ttcatggata
     4021 atcgttggga ttggtatttt agctttggta ttatcgctga atcatttagg agaaaagcgt
     4081 ccagatctag atggtggtat tttcggttac gcggaagctt catttggtaa attaggtggc
     4141 ttcatcagtg gttggggtta ctggttgtct gcgtggctgg gaaatgttgc ttttgcaacg
     4201 atgctgatga gcgccatggg agaatttttc ccgatattca aaggtggaca aaatgttcct
     4261 tctattttat ttgcaagtat ttttatttgg ggtctgactt tattagtaaa caatggagtt
     4321 gaaggagcga gcgtcatcaa tacaatcgtg acgatctgta aattagtgcc attattccta
     4381 tttttaatat ttgtatttat cgcatttaaa gtgaatatat tcactgctga tttttggggt
     4441 aacgtatctg ataatctagt gaatggaaca gggcagcaag gaacgatctg gttgcagatt
     4501 aaaggttgtt tgatggttat gatgtgggtc tttgtaggga tcgaaggcgc aagtgtttta
     4561 gccaaccgcg cgaagaaacg ttcagaagcc cagcaagcat cgatcattgg tctgctttgt
     4621 ttattgttta tctatatcct tgcttcgatc ttgccatacg gcgtcttatc tcaagaagag
     4681 ttagcaacta tttcacaacc tgctatggca aatattttaa aaggaatcgt gggaaattgg
     4741 ggagcagcgc tgatcaatat cggcttgatt atttcgatca ttggttcttg gttgtcttgg
     4801 acgatgctcc cagcagaaac aacgatgttg atggctcgag acggtctgtt gccgaaaggt
     4861 tggggaaaaa cgaataaaaa aaatgcccca acttattcgt tagttgttac tgcaatcttg
     4921 actaatctat tcttattgac tttcttagtt actgattatg cctatcaatt tgcctattct
     4981 ttatgtacag cggcaatttt gatttgttat ctacttgtcg ggatctatca agtccagttt
     5041 tctttaaaag aaggaatcaa aaaacaagta gtgatcggtg ggattgctgt agtttttgaa
     5101 ttgatgggca tcattttagc aggattttct tatgtccttt tatgtagtat tgcctatctt
     5161 cctggttttt gcttctactg gatcgcttgt cgtgaaacca aacatcaaat tacgggaaaa
     5221 gaaaaagtaa tgattgggct gatttgtgtg gccgcactgc tttcaatcgg gttgcttgct
     5281 gtaggctgga tcaagatata agggggaaag aaacatgaaa atcgcaaatt tcggtgtaga
     5341 agaatggcta aataaatggg aaaagaaagc ggtatatgat attgcacaaa gctcgatcga
     5401 agcattgaca ttagacgaac tcgttggctt agatgggact agcgtaagta aatttttcga
     5461 aaaacgcaaa aacgaaccat tggactatgg ctggatcgaa ggatcagatg ttttcaaaca
     5521 agaagtcgct tctctttatc aaaccgtcga tccggaaaac atcttacaaa cgaatggagc
     5581 gactggtgct aatcttttag ctctatacgc gttagtggaa gcaggagacc atgttgtttc
     5641 gatgcttcct acttatcagc aattatatga catccccaaa tctttaggtg ccgctgttga
     5701 ctttgttcat ttgaaggaag aagaagattg gcagtttgat ttagaacagc tagaagcctt
     5761 agtcaaacct gaaacaaaaa tcatctgttt gaatagtgca aacaatccta caggcacatt
     5821 gctagatcgg aaaactttag aaaaaatcgc agaaattgct cgtacagtag atgcatatgt
     5881 attgatagat gaagtatatg cgcctttaac ggataaaggt gaattcttgt cgatcgtaga
     5941 tgtctatgaa aaaggtatag caaccaattc cctatccaaa acctactcca ttccaggtat
     6001 tcgtattggc tggacagcta ctggacctga gttggcagaa gtttttcgaa agtatcgaga
     6061 ctacacgatg atttgcggcg gagtcctgtc agatgatctt gctgtacatg cattgaagaa
     6121 taaagaaaaa atattggcgc gaaacaaaaa gatcattaca gagaaccttg tgattttaaa
     6181 acaatgggta gccaatgaac cgaaagttga acttgtcgct cccaattacg tctctacttc
     6241 tttcattaaa ttagccatcg aagaagatga ccagacattt tgtatcaatt tattagaaga
     6301 aacaggagta ctattagtac caggaagtgc ctttgattta ccgaagcatg cccgactagg
     6361 ttactgttgt aaaaaagaaa ccttggaaaa gggattattc ctcttatctg agttcttgaa
     6421 aaaacaggcc taaacaaaaa cactgcaagg acagtctgtg acaactgttc aagcagtgtt
     6481 tttcatttac gcttattgtc tatgagaagg agagtgccct gaagctatgg agactcctga
     6541 agctttttct tctgcagttt cttgctgatg gatctcttgt gtattgtgtc gtacaagcaa
     6601 gaagttgacc aaaacaagaa gagaagtcga aagaaagacg ccgcgataac caaatgcact
     6661 ggaaacactg gagccgatca taggccctac tacgttacca gttgcttgga aagattggtt
     6721 ataactgaag atccgtccag cagcttgatg aggcgaatat tttgtgatca atgcttggac
     6781 agcagggagc agacaagcat cagagatacc aatcaaaaaa cgtaaggcag ctaattgcca
     6841 aacattcgtg acgaatgcca ttgggatata aaccagaatc gcaaaaccca aaccaatcgc
     6901 aagtattctt tcactcccaa tcttgtcacc taagcgacca aatcttggag cggctatcaa
     6961 agtggcaatc ccaggtatcg aagcaatgat tccgctgatc aacgtaacat tcccatgtcc
     7021 atgcagcaat tggcgaatgt acagactgat gattggacta atggaattgt tggatgcttg
     7081 gatgatcatc gttgtgacga acataccgat gacgacgtgt ggatatttca agtctttgaa
     7141 aatctgtttg gcagaaacca tgttttcttt tttcactggg acaaattttt catggacgaa
     7201 aaacagactc aacaagaaga cgaaaaataa gatgatccct gtaatgaaaa aggtagggcg
     7261 atagccaaac gctgaagcag agatcccgcc aagaagcggt ccaagtaatg ttcctgtaac
     7321 agaaccagtt gctaaggttc caagaacttg accacttttt tcttttggcg tcccagttgc
     7381 gacaagggca gtggcattac tgatataacc agaaaaaatc ccttgaagca gacgcaatgc
     7441 gactaattga tacacactag tcacgcaacc catcaaagaa atgacgattg ccattcctaa
     7501 tgaagcacgc aacagcatca attttcttcc tttttggtcg gctaattttc cccaccaagg
     7561 agagataagc gtagtcacca agaaagtaga tgaaaaagtc aatccgctcc aaaagttcaa
     7621 ttgtgacgta ctataattac ctaacgtatc tatgtaaagt gacataaaag gcatgactaa
     7681 actaaatccg ataccagcca taaatgttcc aaaccataag acaaacaagt tttgttccca
     7741 agtttctctt ttcctaaata atttttcctt tattcttgca gccaattaga ctccttcttt
     7801 ctgaaaattt tcatctacag ctctttatta taaactacaa tatgtagttt atcaatgaaa
     7861 gtctcttttt tctctttctt tttcgtggta aactgaaaga gtgaaggatt tattttatga
     7921 gaggaaacat ggaaatgaaa aaaagaaatc tgtctcaaga gaaaatcatc gactgctttc
     7981 gagaactagc agaagagatg gatgttcagc aaataacctt tcagcattta gccaaacgct
     8041 tggatatcaa ggctccctcg ctgtataacc atttcaaaaa tattcgtgat gtcaaaacag
     8101 cgctaacagc gaaacttctg caggaactta atgaccagct gcgacgggct cttgtgggaa
     8161 aaagtggtca ggaagcactg cagatctatg cagaaactta tcgatccttt gctttttcaa
     8221 atcaagcagt gtatgagtta ctgatcagtg taccgcatac caatgaagct gttttattag
     8281 atggaatcca tgaaacgaat cagatcattt tgcagatatt tgaggctttt caattaacga
     8341 aagaagaaaa agttcatcga agcagggaac ttcgtagtat gatccacggt tatctttcct
     8401 tacgtttttt aggatacttt acaaaagaac caacaatcga tccagaagac agctattttt
     8461 ggatgatcaa taactttatc gctactttgc cttaaacaat cagattcatt tgtaacaatt
     8521 ttaagatgtt cattaaatga cccttttata agagaaaatt cttgcaactt tcttgaaaag
     8581 tgactaagat gagtctagaa aagctgaaag aaggagcacg agtatgtatc aagtaattac
     8641 catgttcggg gacaatgagc cttggtggtt ttttgaagac tgggaagaag atattgaaga
     8701 agaagaaaca ttcgagtctt tcgaagaagc acgacaagcg tacgaaaaac aatggaacga
     8761 aatccatcaa aattatgaat atatcaatgc aaaaagtaac tttctcagcg ccttttggaa
     8821 tgacggagat gagcgctggt gtgaagagtg tgacgatgat ttacagcaat acaagggact
     8881 tgcgttgttg aaaaatcatc agcccatcac agtagaaagc agaaaggaat tttatgaaac
     8941 aactaattct agcggaaaaa ccaagcgttg cgaaagacct aagcaaggtg cttggtgcta
     9001 atcaaaagca caaaaattat tatgaaggac ccaaggtgat cgttacttgg gcccttggtc
     9061 atttattagg attgaaaatg ccggaagatc tgaataagga atggcagagc tggcaaatgg
     9121 acacgttgcc aatgatccct aaaaaactag gaatcaaacc acttcctaaa acaggacatc
     9181 aattaaaagc aatcaagcag ctggctcagc gaaaagatgt ctcagaagca gtcattgcga
     9241 cagatgccgg aagagaaggg gaacttgttg ctcgctggat cttagagtat gtgcatttca
     9301 ataaaccagt gaagcggtta tggatctctt ctcaaacaga taaagcaatc aaaactggat
     9361 tcaagcaact aagacctgcg aaagaatatg atgcattgta tgaatctgct cttgcccggg
     9421 caaaagcgga ctggctggta ggattgaatg tgaccagagc cttaactgtg aagtacgcgg
     9481 ataatttatc agcaggccgt gtgcagacac cgacactagc attagtgaga gaccaagaaa
     9541 agaaaatcga gcagttccgt ccacaaacgt atttcacgat cacgttgcaa gctggaaaag
     9601 aacaagccaa aatgcttcaa aaaaatcaat ttgctttaaa aacacaagaa gaagcacaaa
     9661 aacttgttca attgatcagt acaagcaaag gaactgtcac agaagtccaa gaaaaattaa
     9721 aaacggaatc tgcgccattg ccttatgatt taacagagat ccaaagagaa gccaatcaac
     9781 gctatggcta ttctgcaaag aaaacattaa gtcttgtaca aagtttgtat gagacccata
     9841 agatcgttac ttatccgcgt acagacagca aatatctgac aaatgacatg aaaagtacga
     9901 tgaaagaacg attgcaagca gtcagcgact ttgctcctga ggtcaagcag tatttgaaaa
     9961 acggagccat cgtccaacag acaaaagtat tccaagacag taaagtgacc gatcaccatg
    10021 cacttttgcc aactgaaaac agagcacgtt atgaaaaatt aagtaatgaa gagcgaaaaa
    10081 tctatcaaat gattgtctct cgttttttag gactgtttgc tcaaccccat aaagtcagcc
    10141 aaacaaaagt tacggttgaa tttggtaaag aacgattcat ttttcggcaa aatcgtgtcg
    10201 tccaagcagg atggaaaggc gaaactgaaa ctgagacgga aacagtcaaa tgggaaaaag
    10261 gaatgagaat caatcccgat ttcgccatca aaaaagaact atctgcaccg cctaaaccat
    10321 tgtcagaagc aagcttgctt ggtcaaatgg aaaaatacag tctgggaacc ccagctacgc
    10381 gtgcagagat tatcgagaaa ctaatcaaat ctgaactgat ggaacgtacc cctcaaggac
    10441 tgcaagtatc tcctaaaggt aagcaattat tagtattggt aaatccctct ttagtaacac
    10501 cagatttgac agaaagctgg gaaaaagacc ttgaagctat tgctgcagga aagaaacaag
    10561 caggaacctt cttgaatgga atcgaaaaag aaacaaaacg cttggtgaat gagatcaaat
    10621 caagcaaaca agaatatcaa gatttctcga ttacccaaaa gaaatgccca gaatgtgggg
    10681 ccaatctgcg cgagaaaaac acacgtgacg gcaaaatcta tgtctgcacc aattcagatt
    10741 gcaactaccg cagaagaaaa gatccaaaag tctctaatca tcgatgcccg caatgtcacc
    10801 ggaaaatgga aatcatcgaa ggtaaaaacg gtgcttattt ccgttgtaaa tacgatggaa
    10861 cgacagaaaa attgcttgat aaaaaagagc agaagaagaa aatgaccaaa catgaagaac
    10921 gacgcttgct gaaaaaatat gcacaagcag aagaaccaga agaaagccca ttagcagcag
    10981 cactaaaagc agcaatgaag aagtaataag atatgaaaga ggttgtgata gaagtggtca
    11041 gcttcaagaa ataagaagga atttacgaaa attgcttctc aaatttttgt gaaatttcgg
    11101 cttatttccg aagaagctac ttctggaaca ccatttattc ggaaaaaagg actgtgacaa
    11161 gacttttgtc acagtccttt ttcatttaaa aaaatgttta cttcatttga cgtatattat
    11221 tggtgaggct tcaattcatt tggcttcact ttttctgttt ctcttatgat ataaacagga
    11281 cgctgtttcg tttctaagaa gattttccca atatatttcc cgatgattcc aagacataac
    11341 aattggattc caccaatgaa caggaagatc gagaccatgg aaggccatcc tgatgtagga
    11401 tcaccaaaca acaatgcccg caaaataatg aacaaaattg caataccaga accgacacaa
    11461 gacaaagcgc cgataaatga agcaatcgtt aacggtgcat ccgagaagtt cacgatacca
    11521 tcaatcgagt agttgaataa tttccagaag gaccaagatg tttcgccagc cacacgttcg
    11581 cggttttcat aagaaatata ttctgtttta aatccgaccc agctaaataa tccttttgaa
    11641 aatcggttgt attctgttaa ttctaggacg gcatccacca tttgacgagt catcaagcgg
    11701 aaatcacgag cgccatcgac catttctgta tcgctgattt tattgatcaa ctggtagaat
    11761 tttttcgcaa agaaactgcg tatcgcaggc tcgccttcac gagtcgaacg ccgggtgccg
    11821 acacaatcga tatcagaagt agtgatccgg ttgatcatct caggaagcag ttcgggcgga
    11881 tcttgcagat caacatccat cacggtcact aaatctcctg tagaagcttg caatccagcg
    11941 tacagagccg cttctttccc aaaatttctg gaaaatgaga caaagcggac ataaggataa
    12001 gctgaggcta gcttcttgat tgctgctaac gtaccgtctt ttgatccgtc gtcaataaac
    12061 aaatattcga actcatgggc acctttgaat ttttcaacct catcaaaaaa aagaggaaca
    12121 gttttttctt cattaaaaca tgggacaaca attgatactt tcatcgtttc tatcctttca
    12181 atcgtatgtt tatatatggt ttacattcaa aatttaataa accttaaata acacctttaa
    12241 aatttgattt tacgtaaaag aacaggtatt ctttttgtaa actaacgtat ttagtttacc
    12301 atgtttcctc ggtttatcaa acttttaaga acaattttta ttagttggca agtagttttg
    12361 gatagagaaa aaggaggaag ttccagtcta ttgactagga gcaaacaaaa tgaaaataca
    12421 cacacctatc ggctttttat ggttagatgc agatgaatcc ggattgactg ctgtatcttt
    12481 tcagccaatt tcagaaagcg aatcagcgaa tcagcgaatt ttaacgcgca cacaagaaca
    12541 gtttgaagaa tattttgcgg gaaaaagaaa agaatttgat gttcctttat ctataaagag
    12601 aggtacgcct tttcagcaaa atgtctggaa agctttgtcg accattcctt atggagaaat
    12661 ccgaacgtat aaagaagtag ccgttgctgt tgatagtccg aaagctgtgc gagcgattgg
    12721 gcaggcaaat cgaaccaatc cattgccaat cgttattcct tgtcatcgag tgatcgggca
    12781 aaatggaaaa ctgacaggat atatgggaaa cgcaatagat ggggttgaaa tcaaacgcca
    12841 att
//
LOCUS       DAYYAG010000075        12811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000075 DAYYAG010000000
VERSION     DAYYAG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.075"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHARQ_001899"
     CDS             127..3654
                     /locus_tag="ACHARQ_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8410304.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHARQ_001900"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHARQ_001900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8410305.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHARQ_001901"
     CDS             7442..7783
                     /locus_tag="ACHARQ_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8410306.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHARQ_001902"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHARQ_001902"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8410307.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHARQ_001903"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHARQ_001903"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8410308.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHARQ_001904"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHARQ_001904"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8410309.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4337 a         2236 c         2719 g         3519 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa c
//
LOCUS       DAYYAG010000076        12738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000076 DAYYAG010000000
VERSION     DAYYAG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.076"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACHARQ_001905"
     CDS             97..540
                     /locus_tag="ACHARQ_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8410310.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACHARQ_001906"
     CDS             complement(546..1730)
                     /locus_tag="ACHARQ_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8410311.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACHARQ_001907"
     CDS             1998..2696
                     /locus_tag="ACHARQ_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8410312.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACHARQ_001908"
                     /pseudo
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACHARQ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(3506..4039)
                     /locus_tag="ACHARQ_001909"
     CDS             complement(3506..4039)
                     /locus_tag="ACHARQ_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410313.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACHARQ_001910"
     CDS             complement(4336..6717)
                     /locus_tag="ACHARQ_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8410314.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHARQ_001911"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHARQ_001911"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8410315.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACHARQ_001912"
     CDS             7418..7957
                     /locus_tag="ACHARQ_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8410316.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHARQ_001913"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHARQ_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8410317.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACHARQ_001914"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHARQ_001914"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="ACHARQ_001915"
     CDS             9064..10230
                     /locus_tag="ACHARQ_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8410318.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="ACHARQ_001916"
     CDS             10276..11772
                     /locus_tag="ACHARQ_001916"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8410319.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="ACHARQ_001917"
     CDS             11853..12536
                     /locus_tag="ACHARQ_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8410320.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12545..>12738
                     /locus_tag="ACHARQ_001918"
     CDS             12545..>12738
                     /locus_tag="ACHARQ_001918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410321.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4272 a         2230 c         2630 g         3606 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggttga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttatt
//
LOCUS       DAYYAG010000077        12734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000077 DAYYAG010000000
VERSION     DAYYAG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.077"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHARQ_001919"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHARQ_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8410322.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACHARQ_001920"
     CDS             complement(324..893)
                     /locus_tag="ACHARQ_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8410323.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACHARQ_001921"
     CDS             1079..1981
                     /locus_tag="ACHARQ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8410324.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHARQ_001922"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHARQ_001922"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8410325.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHARQ_001923"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHARQ_001923"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8410326.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHARQ_001924"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHARQ_001924"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8410327.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACHARQ_001925"
     CDS             8549..8734
                     /locus_tag="ACHARQ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8410328.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACHARQ_001926"
     CDS             complement(8796..9992)
                     /locus_tag="ACHARQ_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8410329.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACHARQ_001927"
     CDS             complement(10162..11598)
                     /locus_tag="ACHARQ_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8410330.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="ACHARQ_001928"
     CDS             11891..12700
                     /locus_tag="ACHARQ_001928"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8410331.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2250 g         4010 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtga
//
LOCUS       DAYYAG010000078        12578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000078 DAYYAG010000000
VERSION     DAYYAG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.078"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            294..1019
                     /locus_tag="ACHARQ_001929"
     CDS             294..1019
                     /locus_tag="ACHARQ_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM8410332.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            1009..1683
                     /locus_tag="ACHARQ_001930"
     CDS             1009..1683
                     /locus_tag="ACHARQ_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM8410333.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1971..2255)
                     /locus_tag="ACHARQ_001931"
     CDS             complement(1971..2255)
                     /locus_tag="ACHARQ_001931"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM8410334.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2549..3856)
                     /locus_tag="ACHARQ_001932"
     CDS             complement(2549..3856)
                     /locus_tag="ACHARQ_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8410335.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4345..5451
                     /locus_tag="ACHARQ_001933"
     CDS             4345..5451
                     /locus_tag="ACHARQ_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410336.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            6042..6242
                     /locus_tag="ACHARQ_001934"
     CDS             6042..6242
                     /locus_tag="ACHARQ_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8410337.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            6389..9178
                     /locus_tag="ACHARQ_001935"
     CDS             6389..9178
                     /locus_tag="ACHARQ_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8410338.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            9225..9752
                     /locus_tag="ACHARQ_001936"
     CDS             9225..9752
                     /locus_tag="ACHARQ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8410339.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            9773..10963
                     /locus_tag="ACHARQ_001937"
     CDS             9773..10963
                     /locus_tag="ACHARQ_001937"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8410340.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            11003..12301
                     /gene="asnS"
                     /locus_tag="ACHARQ_001938"
     CDS             11003..12301
                     /gene="asnS"
                     /locus_tag="ACHARQ_001938"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8410341.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         4157 a         2237 c         2566 g         3618 t
ORIGIN      
        1 taacattgaa aatgaatgcg aaaacgatat ttttttttat tggaaaatat cggtcaagca
       61 ataaaccagt tgcataataa aatgttaacg gcaacaaaat ctgtggaagg aatgaaccgg
      121 atgatttgat ggaaaaaaca agatagacaa gaagtataca tagtaaaacg tggaacaagt
      181 aggccagaat gaaaaatcag tttttatcca ctcgcttttc tatacattca ctgtcacaaa
      241 tataatcgaa agtaattcta ttttgagata aagtatttta taggagatag tatatgaaca
      301 aaaaaagatt taaacgtatt gaaagaaaat ttgcattaac ttatgaccaa gctaagtatt
      361 tgaaaacact atttccaaaa caggtagttc gggataaata tagtgatcat tttatttctt
      421 ccttgtattt tgatacaaac gatcatcact ttttaaaaga aatcactaca gaaaagtcct
      481 atcgagaaaa atttagaatc agaaaatatg gagatcctca ggacatatcg cctatctact
      541 tagagataaa aagaaagttt caaggaatga cagataaacg tagaataaaa ttagcgcatc
      601 atgagctcga tgatatgttg gaaaatatcg aattggcaga aaccacattc agtaatttag
      661 aagaagagta tatttttaat gaaatacagt ggttgttcag aaggttggca ttggagccca
      721 aagtatatat ctattattta agagaagcat ttgtaaatag agattcagaa gatttgcgaa
      781 tcacttttga tcaaaatatc cattaccgtc taatgaactt aacgacctgg gaacactgta
      841 cagaagacaa tctggtagcg ccagaaatcg atgtgctgat ggaagtaaaa acgacaggag
      901 aaatacctga atggttacag gtaggattgg ataggatggg taaaaaagag ataaaatttt
      961 ctaaatataa acaagtctat aaaagatata tccaagagga cccgctctat gttcaatagt
     1021 gtcttaaaaa atgagccttc tcaactcaca ctagatgatt tctttttatg tatggtggtg
     1081 tcgattttgc taggcattct gattgcttac acccatatgt atcaaaacaa ctgtacaaaa
     1141 aacttcatgt ttgccttgat tgggttacct actttagtac ataccgtcat tttgttgatc
     1201 aacggtaact taggcacggg gatcgctgtt ctaggcgcat ttaacttagt gcgatttcgg
     1261 tctgctcaag ggaattcaag agatattttc agtatttttt gggcaacgtc gattggttta
     1321 gccacaggga cgggatatat tgtctatgca ctattcgtgt ctagtatagt tgctgctatc
     1381 caagtcatca tcttcacgct tccaatcaaa aataaacata cagtttttaa tactgaaaaa
     1441 acgatcaagc taaaagtttc taaatccttg tttctttcga gtattattga agaaaaaatt
     1501 gcagaatata cagaaaactg caatttaaaa aaagtaaaaa tcaataaaga aacttgttcc
     1561 ttacactata tagtaaaagt aaaaacgagt atgcttgaaa aagaattggt tcgaacgatc
     1621 atggatcatc cagaagtcat ttcgatcact gtaggtatgg ataaatcgaa taaattattg
     1681 tgataaccaa gaaagtactt aacaatgaag taagacccca gttccaaagt ttagcgggga
     1741 cacttcaata aagtactttc ttttttgtct tcagcaacta ctggtaaaac tgagagcaag
     1801 ctttcgtgag agtacagcga taaagatcaa cctccctttt tatctcgagc aatttccttg
     1861 caaggctgtt tcgttgatct taaacgagtt agcaacagtc aaagatgcga tggccataaa
     1921 aaaattgcat taagttggct ggtatctgat agctgggaat ctaaggaaga ctatccattc
     1981 tgtttcttaa attccgctag ttgatagata tcagccaatt gttccagatg atcaacatca
     2041 aagataaccg aaagatgttt ccttttattc ccatttgctt gaagagtttt tatcttctct
     2101 ttggttcctt cactcaatga gagtgtgact aatttctccc tcgttttttc tgggaaatga
     2161 aaggatactt tgaaaaatcc gtcccaaata gaaagccaga agatcggttt tactttctga
     2221 gagtgggaca aaagtaagaa acacttttgt ctcattcttt aaaaacgaat aaacggtggg
     2281 agcagaagca actccttcgg aaataagcca aaattcacaa aaatttgaaa agcaattttc
     2341 gtgaatttct tcttatttct cggagttaaa cacttctgtc ccaacctctt gtattcacct
     2401 tttgatagcc aagcttttcc atcattataa aaccgcaaat ttcaaaaaaa aacgaacaat
     2461 acaaaaagcg tattgctcgt ttttctcttc agtttctcac acctaaacga cttttgtctc
     2521 gacctctttt tggtgactat tcatgatgtt aaaataaatt actgaaaaaa tctgtgacat
     2581 ggcgccaacc aatgacaaaa atattagctt tttcaacagt agaagtggta atgatgtctg
     2641 tcgaaggctc ttggtttgct tccaaatagc ctaatttgtc atcagctaat tggatcgttg
     2701 ctgagccaac tgtagtgcct ttgttgatag gagcggtcac ttggtcatcc gttactttct
     2761 ttttatcaat tgttggagta atcgttaatt ttccagtatc cattccattt tcgacccaga
     2821 cttttacagg gctttttagc gcaacattga cggaggtttc ttttccatct ttcactttga
     2881 tgtcttttag gcttgggatg cttgcatttg cttttccaag ttctttttct gaccagttgt
     2941 cataacaata atccatcaat cgtgatgttt cattgaatcg ggcacttgga ttttcttcat
     3001 ggttatctgc atttaaaacg actgtgatga ttcgtcggcc atctttgttg atcgttccga
     3061 caaagcatgc acccgcaaaa tcagttgtgc cagtttttaa tccatcgaca ccttcgtgat
     3121 agtttacaaa accaggtagc atccagttcc aattgaccat ttcaaccggt gactgtgtgt
     3181 tttcgccgaa tgtttgattt gtggtactcg tgatttttaa gacttcagga taatctaaga
     3241 tgaggtgacg agcaacaata gcgacatctt tcgcagacag ttgattttca tctgtttcac
     3301 cagtattctc aggtcgattc tcacctaggt aagaattgtt taaacctgat gcattgacga
     3361 tcgttgcatc tttgataccc caatctttta gttgtttctt cattagctca agaaatttac
     3421 tttcactgcc tgcaactttt tcagctaaag caaccatgga agcattggct gattggatga
     3481 aagctgagtc aacaagttct ttcactgtat aatcattttc tttatgcaaa ggaacattcg
     3541 atagatccgg cacgacactt aatttttctg cataatccga aatggctacc ttatcttccc
     3601 aagataattt accctctttg atttgttcaa gtacgatata tagaccaatg atctttgtga
     3661 tggaagcaat tcccatcgtt ttttcgccat cctgatcata aaggattttt ccactttctg
     3721 catctacggc aaaggcagct ttggcattta ttttaaatgc gtcgtctgct tgaacagttg
     3781 cgctaggcaa gaaagaagtg atacataaga taagacttgc taatagtaca ataaaacggg
     3841 tatttttttt gaacatcttt gttctcctca tttttaaatt gattcttaca acttagaata
     3901 ttatcagaga gaacaaaagg tttcaatggt tctgaaaaaa accttataaa gaacaagtct
     3961 tattaatgaa gaatcataga aagttatctt ttgggttcat tgacacgaac tttgactggc
     4021 tgaacttcgg gttcggttag gcgtgatgct gcaaaaaggg cgcctgctga gagtgcgacc
     4081 aaactaaaaa tcatgaataa ttgcataaga tcacctcgtt cttttttgtt tttttatagt
     4141 atagcaagag agtttgaagc tactgttaag gaaatgagca tataattgat gaaagtatga
     4201 aaaaaatatg aagtaggaca acacaactaa aaatcgatca ggtatttctc tggtagggat
     4261 tcgttttatt gtattgataa aatgggatgt ggtagacttt atttacagat acaaagaaat
     4321 gattttttta ggaggggtac gtttatggca aaaaaagaaa aagaattatt atcacaattg
     4381 acttcagaag gtaccaaagg accgtttgtc acgattatgt taaacaccca tgttgcgcat
     4441 caagacgtag aaaaagatca aatcaaattc aagaattttg caaaggaagc gaaaaagcgc
     4501 tttgaaaaga aatactctga gcatgattgg tctccattcc aagaaaaaat tactagtttg
     4561 ttggacgatc cgtctttctg gcgtagtgcc acaacgagtg tagctgtcat agtatcacca
     4621 gaacaagtgt ttatccaccg tttgggtatt cctgtagatg accagtatta tgtgagtgat
     4681 ctaccttatt tattagcgat catcaaaaat ggacatttca actattcata ctatttatta
     4741 ggattgaatc gtgattcgat gaaattgtac gaagtaacga ataaacaagt caaagaagtg
     4801 gctttgcctg aagatgcacc aagagatgtc gtcactgctt taggatctga actaacagga
     4861 ggcgggttga actacgcatc aaatggtagt gaaggtacga cgttccatgg agtcaatcct
     4921 aaagatgaag aagtcgaaat tgattgggtc aattattatc aagcagtcga tgactatttg
     4981 aaagatacat ttgaaaatcc agagaaatgg cctatcttct tatttgcatt accagaaaat
     5041 cagacgatgt tcaaaaaagt ggcgaagaac ccttattatc gttccgatgt tgcgatctca
     5101 gcttcacctg cacaactttc attaaaagaa attgaagcag gcgcaaaaaa actatcgcaa
     5161 gaattagctg tcaaagaagc aagtgactac aataagttgc ttgataaaaa gttcattgat
     5221 caactcgttg atattatacc ggcagcaaaa gaagggaaaa tatcccattt attcattgct
     5281 acatctaatt tagcagatgg ttttggcgaa gatccagaaa cagaatatga ccgccgtcag
     5341 gtgctgaatg aactagcaga taatgtgatc acgaacggtg gtcaggtctt tatccttgaa
     5401 caaaaagatg caccagacga aaagagttta gttgcgatct tacgttattg agtgaaaact
     5461 accaaagtag atgaatggtc aggcgttgat agaagtgtat agctccgaga gaaggagttt
     5521 cctctgtttc agccgttgga ttcatcttga gcgagagaca aaagctagct tttgtctctc
     5581 gctcattatt tttgtgcttt agtcccattg gctttgcgag gggaagatac ttttctcaaa
     5641 aaaacgaacc ttgcagttga gagggggata gatgcgcata ctaattttta tcagttaaaa
     5701 aatgatgtgg atcattagag aattccaaag ttcgttcatc ctttttcaga taataaattg
     5761 aaattttttg caaaagtgaa aatagcaaat agaaattttc tgaatcttat aggggtgatt
     5821 tcgagaaaac gttcgtttat ttgcaaatat atcttctaaa taagagaata aatacaaaaa
     5881 aataaggaaa aactctggca attttgtaaa cggtatgata tagtaagaat gtagtttttg
     5941 ttcggtaatc tgtggtagct tgtttcaaat aaacatcttc cagatataca ccattcaagt
     6001 aattgcaaaa tatgtgttga tacacaagga ggatttttat tatggaaaca ggtacagtaa
     6061 aatggtttaa ctcagaaaaa ggatttggat tcatcactgc agaaaacgga aacgatgtat
     6121 tcgttcattt ctcagctatc caaggcgacg gattcaaaac tttagaagaa ggccaagcag
     6181 taactttcga cgttgaagaa ggtcaacgcg gacctcaagc aacaaacgtt actaaagctt
     6241 aatttaccta tattaaaaat aaaagtccag acatttatgt gtctgggctt ttttgtcttt
     6301 ttttccttaa aaagcgatga gagtattttt ctgcactttg tagtataatt tcaaaagaac
     6361 attagaggca ggagaagagg aagatatttt gaaaccaaga acttatgcag tcgttgatat
     6421 cgagacgact gggacgaatc caaaagaaga tcggatcatc caatttggct gcgtcatgat
     6481 tgaagctggt cggatcactt cacgtttttc gatcgatatt catccagggc ggaagatttc
     6541 aaaacaaatc cagcatttaa cagggatcac gaatcaacgc gtccaaaaag cgccttattt
     6601 tgaagatgtc gcccaaacga tttacaatct tttggcagac accatatttg tcgcgcataa
     6661 tatttatttt gattataatt ttttgaacca tgaactcatg cgatgcggac ttcctagttt
     6721 gaagataccg ggcatcgata ccgtagaact tgcacaaatt ttcttgccga cggaaccaag
     6781 ttttcgctta gctgatttgt cagaaagcct tggctttatt catgagaatc ctcatcaggc
     6841 ggatagtgat gccgaagtga caggtcagct tcttttactg atcgaagaac gaatgaggaa
     6901 attgccgatc atcacattag agcagattgc acgattaagt agccatacag gaatggatac
     6961 gagtcgtttc atttaccacg tgcttgaaga aatgaaagaa aacccggagc cccttgatct
     7021 ctcattagaa atcatcgatg ggctagcttt gcagaaaaaa gaagtggagc tattcacatc
     7081 cgtccattat ggcgagcgga catatccaag aaaaaaacaa gcaaaagaaa aaatgtttgg
     7141 taaaacactg atgtatcgaa aagaacagaa caggctgatg aatgctgtct acgaccattt
     7201 cacaaaagat gaatcaaaag atttgatgat cgaagcagct acaggaatgg gcaaaacgat
     7261 tggttatctg ttgcctttga gttatttggc tacacctgaa aaaccagctg tcatcagcac
     7321 cgtttcgctt gttttgcagc agcagatcat ggaaaaagac attccattac tgaatcaatt
     7381 gctagatcag ccgatacaac ctgtgattat caaaagctat cgccactata ttgatttaca
     7441 gcgttttaaa ggtacacttg tggaaccgcc gggacaaaaa caatatgctt tgtaccaaat
     7501 ggctgtctta gtttggctaa cacaaacgaa gaccggtgat ttggatgaat tacatttaac
     7561 caatctgaac catgcattct ttgcagatgt cgcgcacaga ggcactgggt ttttagcaag
     7621 gaatcagtca ttttacaaac aagactttgt gcgtttcttg caaaagaaaa tcaaacaaag
     7681 caattttttg atagtcaacc atgcattttt agtacaagaa acacagcgaa aagaaccatt
     7741 gcttccagct agtccttatt tgttgattga tgaagtgcat catttgccgg aaattgctga
     7801 aaaagtaaat gacaaacgac taagtattaa ctacttccat aaacaaattc agcaactgga
     7861 agagccgaat cagttatttg accgcataca aagttatttg ccgaaggaac atgaagcctt
     7921 gcgctggttg tcgatttatc gagaagaact ctctgcacta tgcgaagcgc agatttgcgt
     7981 ggcagaggga ttaatgaact tagtaagcag caagcaacct aacggacaat atccagaaga
     8041 agtcatgatg acaaaagaaa tgttggatca gctgccactg gaatctggaa cagcaatgga
     8101 tcatctgcaa cttttttatc aagagatcca acaactacaa tcacagatgc gcttgacttt
     8161 tgagattatc caagaaagtt ggaccaacca gatgcggatc gatttagcaa gtttatatag
     8221 cttatttgat caattagaca agcaatactt gctgatggac caatggctag aagactggcg
     8281 agatcatttg atccattggt tcgttcccgg gaatacagca cagcagatga tttttcatct
     8341 ccttgatttt caagcagcta tgttgccgtc aactgtgtgg tatcctcgat atgaccatat
     8401 tctttatatt ggaggaacac tgaagatcgg acctaatcga cagtatctct ctaaacgact
     8461 tggcattgaa gcagcacctc ttaaagtgat tgcttctcct tatgattatg agaaacaggc
     8521 aagaatctat gtgccgaaag aagcacctgc agtccatcaa gtcagcaatc aagcatatat
     8581 cgattattta gaagcagcac tcacaggatt gattgaacaa gaagatcggc cgatccttgt
     8641 tttattcact tctcatgatg ttttgcaaaa ggtatatcag aaaatgcatt tgtctatgct
     8701 agaaaaagga cgtgaaattc ttgctcaagg atttggcggg agtcgggaaa aacttttgaa
     8761 acgcttcatt ttgtcagaaa aaagcgtctt atttggtgct gacagctttt gggaaggcgt
     8821 agatttacct ggagatgctt tgcagattgt tgtagtcaca cgtttaccct tcgacaaccc
     8881 gttacgtccg atggtcaaag ctaggaatgc ttatctggaa tcagagggga tcaatccttt
     8941 ttatcaggaa tccgtgccta aagcagcttt gaagttacgc caagcattag gtcgtttgat
     9001 aaggacggag acagatcgtg gcgtattact tattttggat cggagattag tcactgcaaa
     9061 atatggtgca cgaatcatcc atgcattacc gaaaaaagta ccgataaaag agctagctat
     9121 agatgaaatc tatcaggata tccatgaatt cttaaagaaa aatgaagaag gaagttaaag
     9181 gtgccgtctt ttttcatcat taaaatctgc tataatgaaa aggaatgaag ataaaggagc
     9241 gacgaccctt gaagaaaaaa cagacaaagg aaaaaacgat taatcgcgtg ttgcttggct
     9301 tgatcgtatt tttattagcg gtcatcgtga tcagctcgat tttttacttg cgttccagtc
     9361 gtccggcggt ccaggcgaaa aaagaagcag tagaaatcgc aaagaaatat gcaaatctcg
     9421 aggaagccga tcatttttac tggtttactc gaaagaaaac gtacttttca ttgacgggta
     9481 cgaatgataa aggaaaagaa atcgccgtta tcattcctaa atctggtgaa aaagtaaaag
     9541 tattggatca aacaaaggga ctgactgaaa acgaagcaaa acagacaatt gctgcggccc
     9601 atcctgaaat ccaaatcgaa aaagctgctt tagggatgta tgatgatcag cctgtgtggg
     9661 aagtagtagg aaaagaaaca aatgggaaac tgaattatta cctgattggc tttgaatctg
     9721 gagatgaagt caagacaatc aagggaattt aaaaggtaaa ggaatgaatg ggatggagtt
     9781 atcaaaaaga acaaaacgat tagaaccgtc tgtcactttg gcagctgcgg caaaggccaa
     9841 ggcattaaaa gcaaaaggca gagatgtgct gagtttgact gtgggagaac cggattttgc
     9901 gacaccagaa aacatccaag aagcagcaat cgaagcaatc cgcaatggga aagctagcta
     9961 ttatacacca acagcaggaa tcccagagct tcgacaggca atcgttgatt atctgaagaa
    10021 aaactatgga cttgtctatg aaccttcaca aacagtcgta acagacggag cgaaatttgc
    10081 tttgtatacc cttttccaaa cgatccttga cccgcaagat gaagtcatca ttcctgtgcc
    10141 ttactgggtc agttacggtg agcaagtaaa actggcagaa ggacttcctg tctttgtgaa
    10201 aggggaagaa tccaatagct ggaaagttac tgttgagcag ctagaacaag cacgttcaga
    10261 aaaaacaaaa gccgtaatca tcaattctcc atcgaatccg actggaatga tttatagcaa
    10321 gaatgaactg caggcaatcg gtgaatgggc agtaaaaaat gatattttga tagttgctga
    10381 tgatatttat ggccgtttag tttataatgg caatgagttt actccaattg caacgatttc
    10441 agaagcaatc aaaaatcaga ccatcatcat caatggcgtg tctaaaacat atgcgatgac
    10501 aggctggcgc attggttatg cggtggggaa ttctgagatc atcaatggaa tgattgctgt
    10561 tgcttcccag tcaaccagta atccgacagc agtcagtcaa tatgcagctg ttgaagcatt
    10621 gaaaggcgag caagatacag tggaagaaat gcggaacgcc tttgaagaac gcctgaatac
    10681 gttatatccg cttgtggcag aactgccggg tgtttctctt gaaaagccac aaggagcttt
    10741 ttatcttttt cctaatgtaa aagaaacgct acgcatgtgt aaatatgaaa atgtcacaca
    10801 atgggtagaa gatcttttag aagaaacagg ggtagcgctt gtgactggcg aaggctttgg
    10861 ggcaccagaa aatgtccgca tgagttatgc cactgatcgc gctacgttag aagaagcggt
    10921 acgcagaatc aagcaattta ttgagagtaa aagtcaaaat taatcctgaa gaaattcaag
    10981 gaatataaaa ggagagtcat acgtggaaca aattcaaatc atcgattcga aaaaccatgt
    11041 gggagaaaca gtaacaatcg gcgcttgggt cgcaaataag cgatcaagcg gaaaaatcgc
    11101 atttttgcaa ttaagagacg gaacagcctt tttccaaggt gtagttgtaa aaagcgaagt
    11161 gccggaagaa gttttccaaa cagctaaaaa tctacaccaa gaaacatccg tgttagtaac
    11221 tggagaaatc cgtgaagaca gtcgttcaaa atttggttat gaaatcggaa ttacaaatgt
    11281 ggaagtggta agcgagagcc acgattatcc gatcacacca aaagaacacg gaacagactt
    11341 cttgatggat catcgtcatt tatggctgcg ttcttcaaga caacatgcga ttatgcagat
    11401 cagaaatgaa atcattcgtg caacgtatga attctttaat caacatcatt ttgtcaaaat
    11461 cgatccgccg atcttgactg gttctgcacc agaaggcact acggatctat tcgaaacaaa
    11521 ttactttgat caaaaagctt atttatctca aagtggccaa ttatacatgg aagctgctgc
    11581 tatggctttc ggaaaagtct tttcattcgg tccgactttc cgtgctgaaa aatcaaaaac
    11641 acgacgtcat ttgatcgaat tctggatgat cgaaccagaa atggctttca ctcatcaaga
    11701 agaaagttta gaagtacagg aacaatatgt agcattctta gtacaaaatg ttttagatca
    11761 ttgcgaatac gcattagatg tactaggacg agacaaagaa gtcttaaaaa cttatacaaa
    11821 attgccattc cctcgtatta cttatgatga tgcgatcgaa ttactgaaga aaaatggatt
    11881 tgatgacatc gaatggggcg aagactttgg ttcacctcat gaaacgttca ttgcgaattc
    11941 attcgaacag cctgtcttta tcctaaacta tccaaaagcc atcaagccat tctacatgaa
    12001 accacatcca acaagagaag acgtggtgat ctgtgcagat atgatcgcac cagaaggtta
    12061 cggtgaaatt attggtggaa gcgaacgggc aacagactat gatttcttgc tagaacaaat
    12121 ccgtaatcac ggtttggatg aaaaagaata ttcatggtat cttgatctac gtaaatacgg
    12181 ttctgttccg cactctggtt ttggtctagg ccttgaacga acagtcactt ggattgctgg
    12241 tatcgaccat gtgcgtgaag ctattccatt cccacgtttg ttgaaccgaa tttatccata
    12301 agaggttgtg aaatcagtgc cttgacctga gtattagaga aacaaaacgg aaaaatagct
    12361 tctcatattt tactgttttt tgagctaata tcgagggtca gctgattgaa caccgtttat
    12421 atcaagaggt tgtgcgtctg ccgttcagct ccgagtcaca aggagtaatt tcaaaaaaac
    12481 attttttcat gtttgatgac tattgtgact tgtgaccaag gagttggccg gagagcaacg
    12541 tttatttaaa aaaaagaact gcgaactctc gcagttct
//
LOCUS       DAYYAG010000079        12523 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000079 DAYYAG010000000
VERSION     DAYYAG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.079"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..1976
                     /locus_tag="ACHARQ_001939"
     CDS             93..1976
                     /locus_tag="ACHARQ_001939"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8410342.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2051..2335)
                     /locus_tag="ACHARQ_001940"
     CDS             complement(2051..2335)
                     /locus_tag="ACHARQ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8410343.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2325..3059)
                     /locus_tag="ACHARQ_001941"
     CDS             complement(2325..3059)
                     /locus_tag="ACHARQ_001941"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8410344.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3188..3811
                     /locus_tag="ACHARQ_001942"
     CDS             3188..3811
                     /locus_tag="ACHARQ_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8410345.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4003..5124
                     /locus_tag="ACHARQ_001943"
     CDS             4003..5124
                     /locus_tag="ACHARQ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8410346.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5131..8259
                     /locus_tag="ACHARQ_001944"
     CDS             5131..8259
                     /locus_tag="ACHARQ_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8410347.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8278..11472
                     /locus_tag="ACHARQ_001945"
     CDS             8278..11472
                     /locus_tag="ACHARQ_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8410348.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11618..12325)
                     /locus_tag="ACHARQ_001946"
     CDS             complement(11618..12325)
                     /locus_tag="ACHARQ_001946"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8410349.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4267 a         2212 c         2641 g         3403 t
ORIGIN      
        1 atagtagaga aaggacacga ttctacttgc tattttcact taatacccgt atattacgaa
       61 tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa acaaaaaagg ttaaaaaaca
      121 gccgctacat cactgggttt gatgggctcc gttcattagc tgtggtgggt gttatcttct
      181 atcaccttct gccaactagt atgaaaggcg gctatttggg cgttccgatt tttttcgtcg
      241 tatctggtta tttgatcacg gatctgctgc ggcaggaatg ggaacaaaat ggaaaaatca
      301 atatttggca attttatgtc agaagaatga agagactcta tccggcactt gtttttatgc
      361 ttgtgacagc ttcggcttat atcactttgt ttcagcgagg gctgttaaac aatcttcgag
      421 gagttgtggt cagcagctta ctgtatgtca acaactggtg gcaaatcaat aacggtttat
      481 cttatttcga tcgatttgct aatgagtcac cgtttacgca tatctggtct ttagcagtag
      541 aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt gttgatggta tttgttcgta
      601 aaagaaaatg gatcttttat actgtgttag gtacatcctt cctctccgcc atcttgatga
      661 tgatcttgta tacaccagga ggcgatccaa cccgtgttta ttacggtacc gacactcgtc
      721 tgttttctat atggttggga agtgcgcttg cgtttgtttg gccaagtacg cgtttaaaga
      781 aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc tggaggaatc tcgcttttgg
      841 cactgattct ttgcttcttc tttttagatg atcattacaa tttcatctat tacggaggaa
      901 tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt gacagcacac cccggagcaa
      961 gtttgaatcg atggctgaca aatccagtat ttacttggat tggcaaacga agctatggca
     1021 tttatctgta tcagtttcca gttatgattt tttatgaagc aaaggtgact gatattgccg
     1081 atcatgtttt gttgcatact ttagtggaaa tcacattgat tctaatcatc agtgaacttt
     1141 cttatcgata tgtggagcgt ccgctagcac gttttgatta tggtcgtaca ttccaagtga
     1201 tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa accatgggtt gttccaggca
     1261 cattacttgt agcaattgca ctcgttggtt tcgttacggc accgaaaaac gctgtaacag
     1321 ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa aaaattagca gaagaaacca
     1381 gaaacaattc agaaaacacc actagttcgg ttgaccaagc gatccttgat aagtacgagt
     1441 tgtccaaggc acaagggaat aaggcacaaa aattagaact aacggcattt ggtgattcag
     1501 ttatgttgga tgctgcaacg gatctacaag aactttatcc aaaagtagtg gtagatggag
     1561 atgtcggacg acaattatat gccagtgtac cgtacattga aaaattaaag gaacaagatt
     1621 tgttgaaaga cactgttctg atcggactag gaacgaatgg ctcattctcg gaagctcagt
     1681 ttgatagtgt gatggatgca atcggtgatc gaaaagtata ctgggtcaat gtccgagtac
     1741 cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga acagatgacg aaaaaatacg
     1801 acaatatgac gctgattgat tggtacagct acagtaatga ccatagtgac tggttttatg
     1861 atgatcaagt ccatccaaat cctgaaggga tgacacatta cattcactta gtcagcgaag
     1921 ccttgctagg aggaagttca agtacgaaca gcagtgagac aaacgtgaca gaatgatagc
     1981 tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa gtttttttat atttaccgtg
     2041 tttttaacat tcatatagat tcaggaagca aaacggttga gtgtgtctgt aaataacgtt
     2101 ctttttgttt tcgggtgagt tttttgaatg cagcttctgc ttttgttgct tcgcttcttg
     2161 aagaaaatac ttctgcatgg atcatcttaa caggtcttct tttttgaaga cgagtatatt
     2221 tagcaccagt accattgtta tgttcctcta atcgtctaga aagatcggtt gtatatccac
     2281 cataaaatgt ctgatcttta cagaataata cgtaaaaata atgttcattt gccatagagc
     2341 atctcacgaa tctctgcagt gtattctcca tccatattgt aagtgatcaa tggtggagcg
     2401 actttgaatc cgtctaattt gccttctttg attccttcaa ctaacaaaat atttgcctct
     2461 tttccaggtt ttgggtaaac aaattggatc cttttaggtg caattcgata gtcttccatg
     2521 acctgaatga tttctaagaa ccgatcagga cgatggacca tagtaaagcg tccatttgtt
     2581 tttagtagct tacttgaaat atcgatcact tcttctaacg tcgtatgaat ctcatgacgg
     2641 gcgattgcca ggtgtttatt agggttcgtt ttatttgttg gcagcccttt gaaataaggt
     2701 ggattacaga cgactaagtc acaagaatta tgtcgaacga cagataagct gtctttcaga
     2761 tcgatggtgt ggacagtcat ttgttcattt aaatggttaa gcgcgatact acgtcttgcc
     2821 atgtctgcta gacgaggttg aagttcgata gcgtcgatag ccgcctgggt tcgtttactt
     2881 aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa caatcttccc ttttttaggt
     2941 acacgggaaa agtgagcaag cagcacagca tctaaagaaa aagaaaacac tttggaactt
     3001 tggataatct tgatatcttc tgcgtataat tggtcgattc gttcgtcttc gtgaagcatg
     3061 gattccctcc aaaatgaaaa aaatagtcgt ctgtattata ctatttttat taaaaagaac
     3121 caaggtggct tgtcaaacct ctatttttta tgcatactat gaccataaga agggaaagga
     3181 agagactatg ttttatcgat ttatgcgcgg ggtagttcga gtactattag ccttagttaa
     3241 tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa gatgagaatt acatactagt
     3301 tgctccccat cgtacttggt gggacccatt atatctggcg gtagctgcag cacccaagga
     3361 attcagcttt atggcaaaag aagaactgtt caaaaatcca gttctgcgat ttatattaac
     3421 tcgatccaat gcttttccag ttaaaagaga aaatccagga ccaagcgtca ttaaaacacc
     3481 tgtaaagatt ttgaaatcga caaatttgag tttgatcatg tttcccagtg ggacaagaca
     3541 tgctacagaa cttaaaggcg gaatggcatt gatttccaga atgtctaaag cgaaaatcgt
     3601 gccagctgta tatcaaggtc cgttgacact tggggatctg ttcaagcgtc agagagtgac
     3661 agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag aaaacggata aagaaggtat
     3721 ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa ttggacaaag aagtgaaccc
     3781 tgatttcaaa tatgaagtca aaaaaaaata aaatgataag tttatgcgtg ggcggtcttt
     3841 tgaatctact gtcctgttca gttcagactt tttcaatgaa aacgagggag agacgtcaag
     3901 tctgccctct ttttgcgttc caatatgtaa aatagaagac aggtgtcatg taatggatca
     3961 aagtgttata ataattagaa ggacattttt tgaggtgatt gaatgcgttt tttacataca
     4021 gccgattggc atatcggaaa aaaactacaa ggttatgact tgattgaaga ccagcgtcat
     4081 gtactatctt ctatactaga tatcgcgaaa gaagaaaaag tagaggcaat cgtgattgcc
     4141 ggagacttat atgataggag tgtcccagct gttgatgccg tagaattata caatgaattg
     4201 atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg ctatatcagg aaatcacgat
     4261 agcagtacga gactttcagc gggtacgcca tggttcagtt attcgaacta ttttttgcga
     4321 acacagctag cagaagcatt tgaaccagtc gagtttaaaa acgtacaatt tttctttctt
     4381 ccttactttg aaccaattgc agcgagatta tattttgaag atgacaatat ccgaacgatc
     4441 aagcaagcgg tagccaaggt gatagataaa atgcaggagc tatttgattc agaaaaaaaa
     4501 caagtattga ttagccattt ttttgtaatg ggaagtgtaa aatctgattc ggaaacgaaa
     4561 atcgaagtag gcgggttgga ttcgatccct ggtgaactac ttacaccgtt tgattatgta
     4621 gcactaggac atttgcataa tcaagctgct ttgagacagg ataatgctcg ctatagcggt
     4681 tccccattga aattctcttt atctgagatc aaccaaaaaa aaggcgtatg gatcgttgac
     4741 ctgacaacag gcctggagtt gaattttagg gagatcgaac ccctgcatga tattgaagaa
     4801 ataacagcga gtttcaaaga attgctggat cctgcctttt atcagtccgt caatcgggag
     4861 aattacatct atatctatct cactgataga acgatcatcc cgaatatgat gaaccagctt
     4921 cggcaaatat atcctcgtat tttggggatt gaacgggcaa acggtcgagc agaacaaaca
     4981 aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa aattagcaga agcatttttt
     5041 agtgaaatga cggaagagca gctaactgca aaccaagaaa aatggctcgg agaaacgttg
     5101 caagaaatca tggaaatgga atgatcaaga atgaaaccga aaaaattaca acttaaaaat
     5161 tttggtcctt ttatcaatga gaccgtagat ttttcacgtt tgacagaagc accgcttttt
     5221 ttgatcagcg ggaagacagg agctggcaaa acgacgattt ttgatgggat caccttcgca
     5281 ctttttggtg aaacttccgg aagacttcgc tctggaaaag agatgcgttc tttgtttgct
     5341 acaccagacg aagaaacaag cgtgactttt tcttttgaac atcagcagat gagctatgaa
     5401 atcgaacgaa aaccagaaca agttttagca aaacgaaaag gaaacggaac aaaaaaacag
     5461 gcagccaaag taacgttgac catttttgat gggaaaggaa aagaaataaa acaaatcaca
     5521 aaacgtacag aagcagatca gttgatcaga gaactgatga atctcgatgc gaaacagttt
     5581 tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt ttttggtttc atcaagcagt
     5641 gataaagaga ctgttttacg tcatttattc ggtactcaat ttttccaaca gttcaatgag
     5701 cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg atcatttaga gcaagagctg
     5761 catcttctgc aaaaaaggtt tgttttagtc aaagaagagg aaccagcagc agggtttgaa
     5821 gccgttttag cccaatggac aaaccatcag gcaatactcg aaaaacaaat cacggaagaa
     5881 aaagaagctc taacggaact tcaggaaaaa caaaagcaac tagaaaattc ctactatcgt
     5941 tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat taataaacaa gcaaaaggaa
     6001 ttagctgctc aaaaagacga aatagaggaa aagaaacgat ggattgggta ttatgaattt
     6061 acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa taaaagttga aaaaatagaa
     6121 ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga tgactacgca gaattatgaa
     6181 gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa caaaaatcaa agaagctgct
     6241 caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg aatccttacg tagtaaaaaa
     6301 caagacgcag aacaattaac aatttcattg gataaagaag aattgaatgc cgagaagctg
     6361 tatcaaaatc tcgctatcta tcaggaaaga actcgaattc ttcaagaaaa tttactggat
     6421 caggaaaaac tgcaagaaga tcgtcttgct tttggacagt taaaatatct agatgaaaag
     6481 cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt cgctcgcaca gataagagaa
     6541 gaactcaaaa aagcaactga agaaataaca aaaaatagtc agctattgaa agaacagaca
     6601 gaacaagcag aacagctgaa aagccaatgg gcaaagcagc aaattgctcg tcttcagctt
     6661 tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa cggaacaccc catacaacaa
     6721 ttaaaacacg aacagccaaa taaggaagat atcttaaaag tagaacaaca actggcagaa
     6781 gctgagtctg ctgtagaaag aactcagcaa atcattgcca aaagtgaagc acatgaaaat
     6841 aatcttcaac aacaattatc agatttagaa caaaaaatca tggcaacgaa tatacaattg
     6901 gctgaacaag aagaacactt gcaacaggct ttgaaaaagg catttcctga gataacagaa
     6961 caaaaaacaa aaacaactct tgtagtaatc gaacaatctt tagatagacg aaaaagcaag
     7021 atggccaatg caagaataga gctggaagaa ataagacaac aagcagaaaa gctcgaacaa
     7081 caattagctg tctacaaaca gcagcagaca gaaaaaaata atcagctcca gcaactgcaa
     7141 ggagaaatca aaagtttaga acaacaattg caagatacgg atagtaactg tttagaagag
     7201 gagatacgac gacttgaaaa acagttagaa gaagactcga atcaactagc tgttgagcag
     7261 aaagcaggag tgcaattgtt gcaagatctt catacgcttc ggacacagct tgatcttcaa
     7321 catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg aacaagaaag aatagctaaa
     7381 aagttagaaa gccagtcgta tttttcaaca attgaagaaa tctgtgcatt tgcacaacag
     7441 caagcacaat ataaagaaaa ccaacgtaca gttacttctt atcatgaaga gcaattagtg
     7501 acagctgacc ggctgcgtca attagaaaat atccagctaa ctgagacatt cccagataca
     7561 gaagagatca gccaacagtt agaagaaata agacgtatga tcgaaataaa gcaagaaaag
     7621 atatacggtt tacaagaaca aaaaattagt aatcaaaaaa tctacgatga atgtctttcg
     7681 atttatcaca caagccagac gaaattagat gagcttacgc aattacagca gctttcgcaa
     7741 acattcaacg gagaaaatcc taagaagact agtttggaac ggtatgtgct tcaggtttat
     7801 cttcaagaag tgttacaagt agcaaatgat catctccaac gtttaacgaa aaatcgttat
     7861 caatttgagc tagcggatac cattggcagt tatcgaggga aaaccggatt agaaatcaat
     7921 atctatgatg atcaggcagg tatgtctcga agtgcgcata ccctgtctgg cggtgagagt
     7981 tttattgcag cactttcttt agcattagct cttgccgatg tgatccagac acaagctggt
     8041 ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg ggtcattaga tgaagaagct
     8101 ttggaaatgg caatggaagc tttagaaacg atcgaaagtg aaggaagaat gatcggcatc
     8161 attagtcatg taagagaatt gaaagaacga gtattacaac aaataagaat tgatacagaa
     8221 ggtagcggac aaagcaaaat acgctatcaa cttggataaa aagggagatt ggtaactatg
     8281 aaatggagta ttccagaaaa agtcattgca cgtggacgtc aatacttgca agaaggccga
     8341 gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc atgcagaagt cttgggcagt
     8401 gaattgtata tggttgattt ggatggtaca gcaaaagaga tagattactg ccaatgtccc
     8461 tattgggagg aacataaata ttgtaagcat acagttgctg tggaacttta tttacggtcg
     8521 aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc tagaaccggt aaaagcaaca
     8581 agtgagggag aaatcattgc aaaaggtttt tctcgtctaa aaacacaaaa caatcctcaa
     8641 gcggtgcaac ctttgattat tgaataccaa gtagaaacga ttgagactaa tcagtatcac
     8701 ccagagcttt ctattttggc actttacttg cgtatcggtt cattggcatc accgaaaaaa
     8761 acatatatcg taaaaaacat ctatgatttt ttacaagctt acacagaaaa agaaacttac
     8821 actgttaata aacagtacca gtttcgtcta gataaacggg cgtttcaaaa agaagatcaa
     8881 aaaatactca cgcatcttgc aggaagtgct cagacgcatc aattattagg tacgaacggc
     8941 ttacaagtca aaggaaaact agataaaaga tatctgcttc tcccagtaga acaagcccgc
     9001 ttcctgttag agcagatgaa tcagactaca cgtttgcatc tgcagataaa tgaacaaaac
     9061 gatcaaggaa tacaattttc cgatcaaaac aaacctttat cattcaaagt ggaaaaattt
     9121 ggagaagatt atttgctgca agcattgaat gattttgatt ttttctttat gcattatcag
     9181 tggggagtta tccaagggac attttattct ctgacgaaat atcaacaaac ggtttatcaa
     9241 acttggaaac aattgattcg ccgattggag aaaccagaag tagtgttcaa gaaaaaagaa
     9301 ttaccgtctt tgtttaaaga gattatccct ctgctgtcag agatcggcga cgtagctatt
     9361 gcccctgcag tagaagagga agtagcagat atccccgtcg aatttatttt ctttttccga
     9421 aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct atggcgaagt catctattcg
     9481 actgacccga agcatgaggt ctcgacagaa caggcttttc aggtgttacg tgacacagta
     9541 gaagagaaac gagtacttga tctattcaga atgtatcgct accagaaaaa tgaaactggt
     9601 tatgaacgtc cattgccaag cggggaagaa ctatatgcct ttttccgcac agaactatct
     9661 gtcttccgtc agtttggtga agtcagactt ggaagaaaat tgcgagagtt gtatttagat
     9721 gcgcataaac atcaaccgaa aatcgaagta accgagaccg gttcatggct cgatattcga
     9781 ttcgatgtga cagggataga ggagcaagaa atcgatcatg tcctgcaaag cttgctgaga
     9841 aatgatgctt tttatacatt agaaaatggt caggttcttt cttttgattc agaagaattt
     9901 caacaaacaa gccaagtgtt gcaacaatta cgggaatcga tacgtacaga agaaggaacg
     9961 atccatgtac ctaaaaatca aggattgatc attcaaaatc aattggaaaa aagcaatgct
    10021 acttttagtg aatcgttcca aacgatggta caagatctca ttcatccaga aagataccag
    10081 gcacagcttc ccaaaggatt gaatgcaacg atgagagatt atcaaaagca aggttttcga
    10141 tggttgaaga tgttaggtca ttatcagttt ggcggtattt tagccgatga aatgggactt
    10201 gggaaaacac tacagacaat tgccttttta ctttcggaaa aagaagaacg gaaaagcttc
    10261 tctgctttga tcgtagcgcc tgcgagctta atttacaact ggcaggcaga ggtgagaaaa
    10321 ttcgcccctt ctttaagtat ccaagtaatc aatgggaata aaaaagaacg tgaagaatta
    10381 ctggctaaag acacagatat ccgagtgact tcttatgcca gcttaagaca ggatctagct
    10441 aattatcagt cgcaaaaaat tgattatttg attcttgatg aggcacaaat ggtcaaaaat
    10501 agcagtacga aaactgcaca agcattgaga gaattagctg tacctcagcg gtttgcactg
    10561 agtgggacac caatcgagaa taatttagaa gaattgtggt cattgtttgc aacgattatg
    10621 ccaggatttt tcccgactaa aacaaaattc agggaacttg ccgcagaaga aattgcgcaa
    10681 atgattcgac cgtttatttt acggcgagat aaacaaactg tgttgaaaga cttgccggaa
    10741 aaaacagaga tgaatctata ttctgcactt acagaagaac aaaaaactgt ttatttagct
    10801 tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt ctgaagcatt caagaaaaac
    10861 cggatcggga tacttgctgg tctgacaaga ctgcgacaaa tttgctgcga tcctcgtctg
    10921 tttattgaag attaccaagg cggttccggc aagctcgaac aagtaaaaga tctactggta
    10981 gctgccaaag agaacaaacg aagagttttg ctgttttctc aatttacagg gatgctgact
    11041 atcttgcaag aagagttagc cgagctaggg atctcgacat tttatttaag aggcagtacg
    11101 aaaccacaag atcggttaag tatggtagac gcatttaatg ctggcgaaaa agatgttttt
    11161 ctgatttcct taaaggctgg gggaactgga ttgaatctca cgggagcaga tacggtcatc
    11221 ttgtatgacc tttggtggaa tcctgccatt gaagaacaag ctgctggacg tgcacatcgg
    11281 atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct ctgaaggaac gattgaggaa
    11341 cgcatggatt cactccagca agaaaaacgc gaactgttcc aaaaagtgat ccaaggtaat
    11401 gaagaacagt tgacgaaact gactgaagaa gacatccgca tgattttaag tgtaggagaa
    11461 atcgaggagt aagagactcg aaaaagcgtt cagtaatggg taagaaagcc tttctaaaga
    11521 agcgattgtt tatttgattt aaaaaatagc aaggaggctg ggattccatt ttatcccagc
    11581 ttccttgcta tcattttttt atttctattt ttaaaggtta tacaaagatt gtcttcattg
    11641 tctctaacag ctgatcaaaa gaagaaatct cgatatcgca aattggagtc tgttcaattt
    11701 tgcgttctcg atggttgaac caaagcgctt gccagttagc actttttgca cctagaacat
    11761 cattgtcgta attatcgcca acgtataagg tctcttctgg aagcatatga aattctttct
    11821 cagctagctg aaaaatctct ttttctggtt tttggaaacc tgttgcttga gaaatgagca
    11881 tattgcggga aggaatccaa ttgttcaatt gaagttgctt cagttttttt gtctgatggt
    11941 cagtcggccc atttgtgata attcctaatg ggacattctt ttccttcaag aaatcgaggg
    12001 tttttttgac ctcctcatgc aagcagatat tatcaagttc ttcctcgtaa attttttgaa
    12061 ataagaggcc atcttcttcc gtaatatgag ggtagtctaa atcttttagt gattggctga
    12121 tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc cattactttt gggaagtttt
    12181 catcactatg atggcggaac cggatatata gtggatgcat atctgcatct gaaacaagcg
    12241 gaacgactct tttgaccgcg tttcgaaatg gctgctgctg atcatacatc gtatcatcaa
    12301 catcaaacac aactgattta atcaaaattt tacacgcctt tcggttttca ttgatttttt
    12361 cacaaatcct tgactattgt aacagaaaaa attgtctaaa aaagctgata aataaaggtt
    12421 tttcttacta tatttctatt tccttgacag aataaggttt ttatatttca gtttttttca
    12481 ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa tta
//
LOCUS       DAYYAG010000080        12478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000080 DAYYAG010000000
VERSION     DAYYAG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.080"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1720)
                     /locus_tag="ACHARQ_001947"
     CDS             complement(140..1720)
                     /locus_tag="ACHARQ_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8410350.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2121..3497)
                     /locus_tag="ACHARQ_001948"
     CDS             complement(2121..3497)
                     /locus_tag="ACHARQ_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8410351.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3625..4713)
                     /locus_tag="ACHARQ_001949"
     CDS             complement(3625..4713)
                     /locus_tag="ACHARQ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8410352.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4885..5319
                     /locus_tag="ACHARQ_001950"
     CDS             4885..5319
                     /locus_tag="ACHARQ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8410353.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5397..5930)
                     /locus_tag="ACHARQ_001951"
     CDS             complement(5397..5930)
                     /locus_tag="ACHARQ_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8410354.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6024..7202)
                     /gene="mutY"
                     /locus_tag="ACHARQ_001952"
     CDS             complement(6024..7202)
                     /gene="mutY"
                     /locus_tag="ACHARQ_001952"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8410355.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7195..7992)
                     /gene="recX"
                     /locus_tag="ACHARQ_001953"
     CDS             complement(7195..7992)
                     /gene="recX"
                     /locus_tag="ACHARQ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8410356.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8099..9481
                     /gene="rlmD"
                     /locus_tag="ACHARQ_001954"
     CDS             8099..9481
                     /gene="rlmD"
                     /locus_tag="ACHARQ_001954"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8410357.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9655..10233)
                     /locus_tag="ACHARQ_001955"
     CDS             complement(9655..10233)
                     /locus_tag="ACHARQ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8410358.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10555..11025
                     /locus_tag="ACHARQ_001956"
     CDS             10555..11025
                     /locus_tag="ACHARQ_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8410359.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11133..12359)
                     /locus_tag="ACHARQ_001957"
     CDS             complement(11133..12359)
                     /locus_tag="ACHARQ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8410360.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3680 a         2519 c         2120 g         4159 t
ORIGIN      
        1 attagacatt tctatgattt taatatagta aaaagagccg tcacggaaaa tccgtcacgg
       61 ctctaaacaa aatatgagag gctgtttttg tttggctatt cttattactt gatgcagggc
      121 agcttgtgtc aagacttctt tacatcaaac tttttaattt tacattggga tatttatccg
      181 caaaccagcg ttctgcaaat tggttctcga ataagaacaa cggttggtcg aaacggtcac
      241 gagccaagat attgcgactt gaactcatct tctcatccaa atcttctgga tcgatccagc
      301 gagcgatttt gtgtcccatt ggagacatga ttacttcggc gttatactca tttaacatac
      361 gatattggaa tacttcgaat tgtagctgac caacagctcc aatgatatat tcttccgtta
      421 gatatgtttt ataaagctga atcgctcctt cttgaactaa ttgatagatt cctttgtgga
      481 aggatttttg tttcatgaca ttttttgcag tcactttcat aaacagctct ggagtaaatg
      541 aagggagttc ttcataagca acttttagtt ttccttcgaa caatgtatcc ccaatctgat
      601 aattgcctgt atcatataca ccgatgatat cgcctgctac tgctttttcg acgttttctc
      661 tcgaatctgc cataaattgt gtgacattgc ttaatttcag ttttttattt gtacgttcca
      721 accagacatc cattcctcgt tcgaatgttc cagaacagat tcggacaaaa gcgattcggt
      781 cacgatgggc tggattcata tttgcttgga tcttgaatac gaaacctgaa aattcttcct
      841 cataaggact taccatctga tcatcttgtg ttttgtgtcc ataaggagct ggtgccaatt
      901 caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa ccaaaaaata
      961 ctggtgtctg atcgccacga gcaatttttt cttgattaaa tgcatctcca gcctctttga
     1021 ttagttctac ttcttctaag acttgttcat ataaggaatt ttcagttaga ggatgatttt
     1081 cgggaagcag tccatcttca tccaattcta ctaatcgttc atcctgataa ttttccggac
     1141 gataaaactc tacacgatta tggtagatat cataaagacc ttctagccct ttacccatac
     1201 cgattggcca attcatcgga taagattcga tatctaatag ctcttctaat tcttctaaca
     1261 attccaatgg ttcacgacca tcacgatcta gtttgttgat aaaagtaaat atcgggatgc
     1321 cacgtttttt tacaacttgg aataatttct ttgtctgtgc ttcgatccct tttgcactat
     1381 cgattaccat gaccgcacta tccacggcca tcaaagtacg ataagtatct tcagaaaaat
     1441 cttcatgtcc tggtgtatcc aagatattta ctcgtttccc ttgataatca aactgcataa
     1501 cagaactagt aacagaaatc ccacgttgtt tctcgatttc catccagtct gatttggcaa
     1561 aattacctgt ttttttgcct tttacggtcc cggcttgacg gatagcccca ccaaacaata
     1621 gtaattgttc tgtaatcgtt gttttcccag catccggatg ggaaatgatc gcaaaagttc
     1681 tgcgattgtc cacttgttgc tttaattttg gattattcat aacctaactc catttcttcc
     1741 attcaattat ttatattttc tctaggcaca gattgtccga tctcttaaag aaagacaatt
     1801 tcatataggg ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa acggcgttct
     1861 agagacagcc tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc tgtttttgct
     1921 tggctgctct tattacttaa tgcaggatag ctttttcaca acctttgtca actactacaa
     1981 ctgaatcagt atagcatgga ttcagcgaat gaaaaagcca ccaaaccata agagtcaaat
     2041 atttttttgt cagctgacta aaagaaaaga cgtgttgctt gtttattcaa gcaacacgtc
     2101 tttcataacc aagtttccaa ttatcttctt ggctcgtcat cttcgaattc ttttctgaag
     2161 tatcttcgtt cttcttcagg ttccgcttct gtttcttccg gatcatgaaa aatgacacgg
     2221 attttaagga ttcgagaacc ttccatttct tcagaaacca gcgtaatatt atcgacatca
     2281 aacgataact tttcgccttc atccggaatc atgcctaatg ctgtaatcaa ataaccagcc
     2341 atcgtatcta catcactcat atgcagatca ctgtcaaaag cttcattgaa ctcatcaatc
     2401 agcatacgtc cttggatgat atattcgtgt tcatcgattt tttcataaag attctcaact
     2461 tcatctgatt catcatcgat ttctccgacg atttcttcta aaaggtcttc tagtgttgct
     2521 aacccaacga cgccgccata ttcatctaat aaaatagcca tctgattctg cgtctttttc
     2581 atttcatata acagatcatc aataaagacc gtttcaggaa cgaataaagg ctcctgcatg
     2641 attttcttga tatctaaatt atcaaagccg aatttatggg cagcttttag taaattcttt
     2701 gtatgaagga ttccgactac tttatctttg tcttcgttat aaacagggat tcttgaatag
     2761 ttttcggata aaacttcatt cacattttct tccacagtgt cgttgatatc aatcataaat
     2821 gcatccgttc gtgggaccat gacttctcgg gcaactttcg tatcaagaga aaaaacccct
     2881 tggagcattt ctaattcttc attattcaat acgccttcgt tttcaagcat ataacgcatt
     2941 tcatctctgg tcatttttga atcttcatca tcaaaagtca tcggcgtgat acgcgccaat
     3001 aaacttgtgg aagcagaaag cagccagacg aagggcttag ctactacacc aatcaagcgt
     3061 atcagccctg aagtgaactt tgcgacttct tctgatttat ttaaagcaat acgtttaggg
     3121 tacaattctc caaaaacaat cgatacatag gtcaaaatag ccagcacaat gatatttgca
     3181 atattttttg ccgctgtgcc gccacctaat accggagcta accgttccga taaagtatta
     3241 gctagtgaag caccagacaa aatgttgacc aaagtgattc ctacttggat tgtcgatagg
     3301 aaattatttg gattctgcaa tatttttaaa agcttttgag atttcacatc tccttcatca
     3361 gctttttgct cgattcgatt cttattgatc gaaacaacag caatttcgga agctgctaaa
     3421 aaagcattga tcaatgtcaa aacgatcaat aataaaatct gcgctaacag cgactgactc
     3481 tcagggtcag cattcataat cgcattctct ccttataaaa ttaattttta gtttacgata
     3541 gaaaaaaacg tggcactata gccacgttaa aatggtatca caaagcaatt ttctttgcaa
     3601 taaaaacaag tgtactaaaa ctacctacga ggtcgtagca attttcttct tgttttcatt
     3661 ttgcttgtct tctttgacga ttttgattat ataggaaatg atcgtcttgc agattgcgta
     3721 aaaaggaaca cctaagaaga taccaagcaa tccggcaata tttccagcta ctagaagaac
     3781 gataatgatc gttaatggat ggattttcag tgatttccca attacattag gataaatgat
     3841 attcccatcc aactgttgga cgattagcac aaccacacaa caaagtaatg cttttaccgg
     3901 atcattgaag actgttacta gaaatgcagg tgctagtcct agataaggtc ctaaatatgg
     3961 aattaggttt gtcaatcccg caatgacccc aaataagaaa gcataatcca caccaattac
     4021 catatatcct aaaaatgtga aggtaccaac gaataaacat tcgattgctt gtccgctaat
     4081 gtaattagcc aatgttttat tcagttgccc caataactcc acaatttggt ttcttctttt
     4141 ttctgggaaa aactgttgga tgtttggcac tagtttttct ccgtctttca acatatagaa
     4201 caagatgaat ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc caacgatcga
     4261 acctaaacta ttggatactc cactaaggaa ttgctgaatg attgtcccgt aagaaatgtc
     4321 taattgctca aaatatttat tgatatctac ttccttgaac aaaggcaatt cggctacctc
     4381 ttttaaccag ctttctacct gtttgatgaa atcaggcata ttagatgcca atgaggaaat
     4441 ttgctgtact agacttggga tgacactaag taaaatccaa ataattgctg caactaaaag
     4501 gagcagcaca attgcgacag cccatattct tttgattttt gctttcatca acagcttgac
     4561 aataggattc aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa ataacgtgga
     4621 aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct gatgaaatca tgatcaatgc
     4681 agctatgatc aataattcaa gcgaccaaaa cattagttta gactgtctaa gtttttcaaa
     4741 caatagattt cctcctttct ttcttactag tatatattat attcgttaaa aattctttta
     4801 ttcttttttt atttttgaaa acttgccata ctttttattt gttttatttt ccagtatact
     4861 atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa caaaagatac aatgagcaat
     4921 atttacttag atgctgtacg gatcaggcat caagtttttg tagtcgaaca gggtgtgcct
     4981 ttgtctcgag aaatagacaa agatgaagcc cattgtatcc acttcgtcct gtactcagat
     5041 aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa aatgaaatta
     5101 cagcggatgg cgattctttc tgaatacaga caccaagggt taggaaaaat attgattgaa
     5161 gaagcagaaa actttgctaa gaatcaaggc tataatacga ttttattagg ggcacagtcc
     5221 acagctgaaa ctttttacga aaaactagga tatacagctt acggcgatcc ttttgaagat
     5281 gctggcatgc ctcatattta tatgaaaaag actttataaa aatttatttc gaaaaaagca
     5341 aaaaacagca agcttttgag aaaaatctct cactggctta ctgttctttt ttgtgtttac
     5401 ttacgcgaca attgcttgta acggttatac caaatatcaa tatagccttc cgagaacggg
     5461 ccatctccat tattgatcca gtcaaccaga atcttcacat tttcttttag gatgaagtca
     5521 atatcgttcg ggtaattcat cattttgctg tgcatctcat attcatcgac atccaatagt
     5581 cgtttctcgc cgtcaggaaa taccttgata tccaagtcat agtcgatata tttcaaggcc
     5641 tcatcgtcca gcaaaaaagg tgaagctaga ttacaataat aggaaacccc cttttctctg
     5701 atcattgcta ttacattgaa ccagtatttt ttatgaaaat aaacaatcgc tggttcgcgc
     5761 gttacccatc ggcgtccatc agattcagtg actaatgtgt gatcattcac gccgatcata
     5821 gagtgttcgc ttgtttttaa taccatggta tctcgccacg ttcgatgcaa tttgccgtcg
     5881 tgcttgtaac tttggatcgt cacaaactct ccttcttttg gaactcccat agcaaccctt
     5941 ccttaagcag cattttgtgc tttgtgtttg ggtgttcatt acaccgacat cataattatt
     6001 atatcaataa aaatgagctt tgcctagaaa ggattcctat tgtttcgatt tttctctaac
     6061 tgatctacca gtttcatttg gaccttagga aaaacgaccg aatcaaatgc atcaggcttc
     6121 aaccacttcg aagttttgtt ctcggtaaat tcttgttctg ctccctcggt tgcccgtccg
     6181 taaaaaagga gaacatgcca tttcagatga ctaaaaatat gagtaacttc tcctaaatgt
     6241 cgtgtctgcc aaacaaagag ttcctgcttt tcaaatggga gattttgctc ttcttctgct
     6301 actaggtcat caaacagatc gatttctggc tttgtttccc attctttttt caatcgctca
     6361 tactcctctt gtgtcacttc catcattgga aatgtccaca tattcgccag caatttttgg
     6421 ctatctcttt cttcaaaata atacgctcca gaatgatttt gaagagctgc actgatataa
     6481 taaacgtctt ttggctttgc tttttttgtt tttacaggaa aactcgtttg gattcctcgt
     6541 ttgttagcta aacaaaaggc ttggatcgga caagtctcac actttggaga agttggtgta
     6601 cagatagctg aacctagatc catcattgct tggttgaatt cacctggatg cttttcatca
     6661 atgattttcc gcatcgcttc atcaaagatt ttcctgctag aagcttttgc gatgtctgct
     6721 tcaatgcaaa acaatctgct gactactcgc atcacgttgc cgtcaactgc aggttctgga
     6781 agtccaaatg cgatacttgc aatcgcgcct gtcgtatatg gtcctattcc cttcaatgag
     6841 ctaatttctt caggcgtttg cggcatttcc ccatcaaatt ctgacatgat ctgtttagca
     6901 gcagcttgga tgttccgggc tcttgagtaa taaccaaggc cctcccatgc ttttaaaagc
     6961 ttttcttctg gagcatttgc tagctcttca attgtaggaa accattccat gaaacgataa
     7021 aaataatcga tcactgtgtc tactctggtt tgctgaagca tgatctcaga gatccagata
     7081 cgataaggat cacgattata gcgccaaggc aggtttcttt tttcttgttc ataccattga
     7141 atgaactgat cttgaaattc ttttgtttct ttatctgtcc atttttccca ttctttattc
     7201 actttcctct tccttttccg taatgaatcg ctggatcata tccaaatcaa atcctttttg
     7261 atataagctt tgctttactt ttagatttct ttttttaggt tcaaacctta aatttcttcg
     7321 ccatagttta tctccttgtt tgactaaata atcatattct atttcttgat ctgcttcttg
     7381 cggaagatca gctatcgctt gttggatcac ttcttgagaa aagcctttcg tcatcaatgt
     7441 ttgttggatt tttctcaata attctttttg gctttttccg tgattttttc caaacgcttt
     7501 ttcggctgtc tgatgtgcat tttgaacttg gtcttcaaat gtatattggc tgagcgcttg
     7561 ttcaatgatt tcaggtttca aacctttttg ctgcatctgc tgcgctaatt tctttggtcc
     7621 tttgtcactt aagcgcatat tcgtgcgcac ataactttcc ccatagacca aatcatcgac
     7681 aaggtccaat tcttttaagc gggctacgat tttatttcga tcttcttgaa ggatttcctt
     7741 ttcttttaag tatgttcgga tttctttttc cgatcgcaac tgataactta agtaattcat
     7801 tgctaggtga aaaccgaaat cagctccgct actcttcttg atctcttgga tagtttcttc
     7861 gtctaattcc cgtcctttta ataatcgata gcgtactaat acatcttcag atacttgtaa
     7921 agcttctcct tcagataaat ctacgcgata gaaagggcct ttgcctttac tgattctcgt
     7981 aacggttaac ataaacatcc tcctcgaaca tatattctct atcataccac agaaaatccc
     8041 cgtaatcagg tattatatgt tagaataaac cacagaaaaa agaaaaaggg agatacttat
     8101 ggcagaaaca attgtcaaaa ctggacaaac ggttcctttg aaaatcaagc gtttaggcat
     8161 aaatggtgaa ggaatcggtt atttcaaacg gttgatcata tttgttccat atgcactacc
     8221 aaaagaagaa gtactagtga aaatcacgaa agcaactcct cgctatgcag aaggagaatt
     8281 aataaaagtc aaaaaaacaa gcaaagatcg cgtagtggca ccatgtcctg tttactatga
     8341 atgcggtggc tgtcaattac aacacttagc ttatcaagct caattggatt ttaaaaagga
     8401 tctgcttttg caagcattgg aaaaattcaa accagcaggc tttcgatctt atcaactttt
     8461 gccgacgatc gggatggatg agccttggca ttatagaaac aaagcacaat ttcagttacg
     8521 aaaaaataag cagactcaaa aaattgaagc tggtttatac gaagcaaact ctcatcagtt
     8581 agtccctata acagactgct tggttcaaga acctcgaaca caaaaagtaa tgaatactgt
     8641 tgttcaacta ttgaataaat ttgatgcacc catctatgat gaacggacaa acagtggaat
     8701 cttccgcaca atcatggtta gaatcggtgt taaaactggg gagttacaag ttgtttttat
     8761 tactcgcagt aaaaaattcc cgcaaaaaaa cgcaatgatc cgtgagatca atgaacgact
     8821 gcccgaagtt gtttcaatca tgcaaaatgt ccaggcacaa aaaacgtctc tcgtgatggg
     8881 ggatgaaacg atccacttat ggggaaaaga agccattgaa gaacaaatca atgaagtgac
     8941 tttcgatctt tctcctcgtg ccttctttca actcaatcct cagcagaccg aattgcttta
     9001 tggcgaagga atcaaagcat tagatgtcca gcctaatgaa acgatcgtgg atgcttattg
     9061 cggagttggg acgatcggct taagtgtagc aaaacaagcc aaagaagtac ggggaatgga
     9121 taccgttcct caagcaatcg ctgatgcaaa aaaaaatgcg gaaagattag gatatcacaa
     9181 cactcgatat gaagtcggga cagcagagca agttctgcct aaatggttga atgaaggttt
     9241 tcagcctgat ggtatcatcg tagatccacc acgtacgggt ttggataacg cattgatcca
     9301 atcattattg aaataccctc caaaaaaact tgtttatatc tcctgtaatg tttctacact
     9361 tgccagagat ctggttaatt taagtaaaaa gttcaagatt gaatatttac agtccgtcga
     9421 catgtttcct cagactgcac gttgtgaagt tgtcgtgaag atgacgagac tcgctgacta
     9481 aatgaaagag gttgtgaaaa agtctgccct gtatcaagta ataagaacag ccaaacaaaa
     9541 atagctcttc atgttttatg aatactgtac ttgtgaccga agagttaaca ggaggacact
     9601 atttatttaa aaaaagggtt gtgacatagt ttttgtcaca gccctttttg attattattg
     9661 gatattcacg tctatcactt gataaaatgc atttgtcgta tcgctgatat tccatactgc
     9721 taaaatcaca ttatatcctt ttcgatcctg cggaagtgtg atcgtttggt tgactgtttt
     9781 tggtggaaca gttgctttgt cgtctatttt tgtgatcaat tcaaaatcac taaatttcaa
     9841 aggttgatta ggattccaag ttggtttggt aatgtagtaa tcccaagtac ttgtccgatg
     9901 ttgtgctgtt aaattccaag taatatttaa tgttcctgga ttaaccatgt ttttatgcca
     9961 tctgttagat gtctgttcat ctaaaggctc aaagccgctg actcctgcac tagcaatttt
    10021 tccatcaatg aatgtatttt tggtagcttc gatactttgc ggttcccatt gtgctcttcc
    10081 gacattttga tttaagtttc ctgctgctga tgttcctagg tatgcacggc ttcctggaga
    10141 agtaatataa ccatgcgcaa aacctggaat gctaaaaccg aacaacccta aactaactaa
    10201 cccaataaac aataacgtac taatcttttt catgtagaaa cctccttcat ttgataacca
    10261 cattgtaaac gctttattgt attaacacaa acattatgct tttataattt tatgttgaag
    10321 taaaaaaggt gaaaaaagta agatgcagat tataacagaa gtagacgaga aaagtacaaa
    10381 tagaaaacat gagaatcaaa tcatatgatc tttttcaaaa acatcattca tataaacaaa
    10441 tgttcaactt tttaataaca acaattatca atttttttct tacttcacaa actaaataca
    10501 ggtcttgctg tgagccttca tggtaaacta atattataga aaggagttag taaaatgaag
    10561 atgataaaag atattcgttt ttgggtttgt gttataggaa ttgtcatatt aggcttttta
    10621 tcagggttgc ttagcggaaa tcccggaaaa tattactatt ctttgcaact tccaccattt
    10681 gcaccaccta gctggatatt tgggccgatg tggaccttac tgtatatatt gatgggtatt
    10741 tctctttatt tgttacttaa tcacaacaac aaaaaacagc gaaacaatct tgtcggtctt
    10801 tttgtgattc agttcatttt taattttata tggtctgctc tattttttaa tttaagaaat
    10861 attttcattg cagcaatcga tattacactt ttagttattt tcttgtcagt gcttatgtat
    10921 cagttgtggc tgcatcatcg cctggcaatg tggctcatga ttccttatta tttatgggta
    10981 ctattcgcaa ctctattgaa ttattcaatt tattttttaa actaagcgct aaaaagagac
    11041 gtcctgtaat ttaacacata gaaaaagtgc cacctaagtt agattttttg tctaatttag
    11101 gtggcactct ttattaacca aactattttt ttctagtacc cacttgttgt tccatactca
    11161 ttattctggt cataaccata accattttgt tcctgtccag cgtaaggatc ataactttga
    11221 gtatcttgac ttggtgtata gtactgactt gtatcttctg tttgctgata ataggtattc
    11281 ggatcttggt actgatcata tgtgttttct gaataggaag aataatcggt gtacccttca
    11341 ctattcgatc catcaccgtt tgtcgaatcg ccatcttccg aatcatcaaa gaacatcatt
    11401 gaagcattct catttttcaa attaggtaat gtatcgcttg tttttatatt taattgtttc
    11461 ttcagttcat tttggatcga aagaagatca ttttttccta aaatttgata gtagatatca
    11521 ttgatcattt gtccttcgcc ttgcaactgg tcttgtttga ttgtttcaaa cgccggggtg
    11581 tagttcgtcg ctacatcaag catgtcatcc caatctaagt ccgtcgtcac attgttctca
    11641 actgctttta aaatttttct ataattgctt acgccatcta aacttaaaac tttattgacg
    11701 attttcgtca ctacttcccg ctgacgacgt tgtcgaccga tatcgccttc tggatcttcg
    11761 tgtcgcattc gagaataggc taaacctttt tctccattca atgtttgttc acctactggt
    11821 acatggacac catccaaagt aaagtcaatt ttattattca ctttgatgcc accgaccgca
    11881 ttgatcaaat cttccatgcc atccaaattg atcgttacat aatgatcaat aggaatatcg
    11941 agcagttgct caattgaatc catggccatt tcgacaccac caaatgcgta ggcatgattt
    12001 aatttatcta ccgttccata acctacgata ttcgtataaa tatctcggtc caaactgatg
    12061 atcgttgatt ttttttgttt cggattaact gttactacca tcatcgtatc agaacgtcct
    12121 tgttccgttc ttcctaatcc accggtatct aaacctaata gcaaaaccga aaaaggttct
    12181 ttgtcatcaa tgcttactct ctcagaacgt ttggaggata ttcgattaga gtttttagaa
    12241 attttgttta ctgtttggct cgcttctccc ataaatctga ttccgtatgc tgataatccg
    12301 gcaatcagca aaacgaatac ccctaacaag gttaatagaa ctttttgcca tcgcttcata
    12361 atacacctct gtttcacatt ttctaaaata ttgtctcata taactgttca atcagcaaat
    12421 tcacttttta atcataagaa aactttaaaa aacatttttg tgtaacgatt attatttt
//
LOCUS       DAYYAG010000081        12439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000081 DAYYAG010000000
VERSION     DAYYAG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.081"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(339..1508)
                     /locus_tag="ACHARQ_001958"
     CDS             complement(339..1508)
                     /locus_tag="ACHARQ_001958"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8410361.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1712..2887
                     /gene="mltG"
                     /locus_tag="ACHARQ_001959"
     CDS             1712..2887
                     /gene="mltG"
                     /locus_tag="ACHARQ_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8410362.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3023..3502
                     /gene="greA"
                     /locus_tag="ACHARQ_001960"
     CDS             3023..3502
                     /gene="greA"
                     /locus_tag="ACHARQ_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8410363.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3671..4402
                     /gene="liaF"
                     /locus_tag="ACHARQ_001961"
     CDS             3671..4402
                     /gene="liaF"
                     /locus_tag="ACHARQ_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8410364.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4399..5466
                     /gene="liaS"
                     /locus_tag="ACHARQ_001962"
     CDS             4399..5466
                     /gene="liaS"
                     /locus_tag="ACHARQ_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8410365.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5473..6105
                     /locus_tag="ACHARQ_001963"
     CDS             5473..6105
                     /locus_tag="ACHARQ_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8410366.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6245..6910
                     /locus_tag="ACHARQ_001964"
     CDS             6245..6910
                     /locus_tag="ACHARQ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8410367.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6938..7231
                     /locus_tag="ACHARQ_001965"
     CDS             6938..7231
                     /locus_tag="ACHARQ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8410368.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(7299..8246)
                     /locus_tag="ACHARQ_001966"
     CDS             complement(7299..8246)
                     /locus_tag="ACHARQ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8410369.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8424..9227)
                     /locus_tag="ACHARQ_001967"
     CDS             complement(8424..9227)
                     /locus_tag="ACHARQ_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8410370.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9373..10287
                     /locus_tag="ACHARQ_001968"
     CDS             9373..10287
                     /locus_tag="ACHARQ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8410371.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10335..10805
                     /locus_tag="ACHARQ_001969"
     CDS             10335..10805
                     /locus_tag="ACHARQ_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8410372.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10890..11498
                     /locus_tag="ACHARQ_001970"
     CDS             10890..11498
                     /locus_tag="ACHARQ_001970"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8410373.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11632..11832
                     /locus_tag="ACHARQ_001971"
     CDS             11632..11832
                     /locus_tag="ACHARQ_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8410374.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4184 a         1908 c         2495 g         3852 t
ORIGIN      
        1 atttctataa tcacgctaat tgttcttctt ttttattgca taaaattaaa aataagaaca
       61 agccatgaat gatttgctag cacatatttt agcaatctaa agatactatt ttagatttaa
      121 taggcccttt gtcaaataat gttggtaaag agaaatctta gaataatttg gacagaacag
      181 aaagaagaac acctctttct gttctgttta tttggaatta aagtctgtag caaaaattga
      241 tttttagatt tgtcccacac tctttcctac gtttagtcga gtactttttt taagcaaatg
      301 ctattcaaag aagtagtatt tgctatcttt tcatcatatc agctatttgc caacttagta
      361 gttccttcca tcaggtcata gatttggttt cttagatcag cagtaggcag tttgatagtt
      421 ttgtcagctt ttatattgct ttccatgatg atagcatcct gtgaatcttt gctaaatata
      481 gcagatgtat caaaagagtc gatatttcct tgcgacagga tataattatc tccgctatat
      541 accccaccac tatatggatg tccattcgca ttttcacgcc ataatgagaa gagtaagtct
      601 ttggaaacta gttttccaga taaaacttgt gtaaacagcg tatttaaatc atagacggac
      661 atactgatat taccagtccc taattcgtta tggatctctg tttcagattc tgtcagtggt
      721 ttaaagtaat catcctcttt agacatttga taggatagtg catgattgga tgaatttttg
      781 acttggtcat aaaattttgt atgctgtaat cctaaaggtt cgatgatatc atcggtcaat
      841 aatttttcat acggttgatt tgttaatttg atcaaaattc cagtcaatat ggtaaagttc
      901 aatgaatcat aatgagaggt ctgataatct gtaaatttta agtgctgaat cgcaaattga
      961 acgacttctt catcagtcat cggtatggtt ggagaagcag ttcggtgcaa gcctgagcgc
     1021 atatacagca agtcacggat agtaatttgc tggctgccag caatttgtgg atagaattga
     1081 gccagcttag tatctaaaga aaggtgccca cgctcgactt gttttaaaat caaaagagta
     1141 gtcaatgctt tttgaaccga accaatctga taataaagag aaggatcatt tggttgttgc
     1201 gtgagtttat tcgcatagcc atagcctttt tccatgatga tgcgattatt tttctttacg
     1261 taaattgagc caatataatc attttttctt aatagttgat cgattttttc aacgagctgt
     1321 tgttcattgg tagtcagtat caactttttt tgagctgatt gagcatgggc aactatctga
     1381 gagtgttcat tattgtaaaa ttgccatcca agaatagttc ctgtagagaa cgcactaagg
     1441 ctaaacaaca cgacaacgat cattaaaaat gaatattttc tttttttagc atgttttcct
     1501 tttttcataa tagtttattt agtctccttt cttttttaca gaaagaataa agctaaaata
     1561 gcttgtatct tgataacatt tattttaatc ttatagtaat ggatttacta cttacatttt
     1621 tgatagaata tttaaataaa atcattcacg ccaattcaaa tatcatgtat aatgaataaa
     1681 gttacgaact tgatagtaag gaggagttgt tttgtcagga aacaaacaac acccaagtaa
     1741 agataaaaaa gaagccagta aggcagctac agcagatata atcgacaagc ggagaaatct
     1801 gcgtaaaaaa gaagataaga ttgtgaggaa gatcatttta gtgattgctc ttacattatt
     1861 gattattggc ggatttcttg gatttaccgt gtatcgttat gtagatagcg ggttgaagcc
     1921 tttagataaa tcagatgacc aattagtcca agtcgagatc ccgagtggct cttctaacaa
     1981 gcagattggt gagattttgg agaaggacaa tattattaaa agcgggattg ttttcaatta
     2041 ttatacaaaa tttaaaaatt taactggctt tcaagctggc tattatcagt tagcgcctaa
     2101 tatgactctt gacgagattg gcaaacagtt gcaagaaggc ggtacttctg agccaacaaa
     2161 agttgcagat gggaaaattg ccattccaga agggtacgat atcgatcaga ttgctgaacg
     2221 ggtagcgaag gtaactggta aagacaagaa agaattttta gatttagtga atgatgagac
     2281 tttctttaac gagttgcacc aaaaattccc agaactgttg gaaagtgcat ctaaagctga
     2341 gaacacaaaa taccgtttgg aaggttactt attcccagcc acatatgatt attacacgag
     2401 tacttcgctt aaggatctag tgattgaaat ggtgaacaag acgaatacgg tcatgcaaaa
     2461 ctattattca gctatcaagc agaaaaattt gactgtacaa gaagttttaa ctctagcttc
     2521 tctagttgaa aaagaaggcg taaaagaaaa tgatcgtaaa aacattgcac aagtcttctt
     2581 taaccgaatc aaagcaaata tgccacttca gtcagatatc tctgttttat acgcattagg
     2641 tgaacacaaa gaattagtga cgtatgaaga tacagctgta gattcaccat ataatttgta
     2701 taccaatacc ggctatggac ctggaccttt tgacaatcca agcgaagagg caatcaaggc
     2761 agtattagaa ccagcagaaa atgactatta ttatttcgta gcagatacga gcactggaaa
     2821 tgtttatttc gctaaaactt atgaagaaca tatggaatta gtggaaaaat atgtaaataa
     2881 ttcttagaaa aacaggctct agacattgat catttttaga aaaacattta catttgaaga
     2941 aaagcgtggt aatctttttt ggactatatt cctcaaaaga ttaccttttg cggtatttta
     3001 tcgaaaaaag gagacgttac acatggtaga gaaagtttat ccaatgactc tagaaggtaa
     3061 agaaaaatta gaaaaagaat tagaagaact aaaaacagtc aaacgcggag aaatcattga
     3121 acgaatcaaa attgcccgcg gtttcggaga tctttccgaa aactctgaat atgaatcagc
     3181 gaaagatgaa caagcatttg ttgaaggaag aatcacaaca attgaaaata tgattcgttt
     3241 tgctcaaatc atcgataacg acggtgtaga tgcagatgag gtatcaattg gtaaaacagt
     3301 tacattcatc gaactaccag atggagaaga agaagagtac acgattgtcg gaagcgctga
     3361 agcagatccg ttttctggca aaatttccaa tgattctcct attgcgcaag cattgattgg
     3421 caaacgtaaa aatgatgaag taactattgc tactccaggt ggcgatatgt tagttaaaat
     3481 cgtcaaagta gaacaagcgt agtctgactg aaagatctag agagttcatc ttactgatga
     3541 tacactctag atctttttct ctttgtgtat tttagatttc aacaaacgat cttacatcta
     3601 aagtcctata gccaacagtt aaaaaaatag gtatgatgaa atgagaatgt gtcaggaggg
     3661 agaaagagcg atgaatagtt cttggcgttt ttttgtggta gttgaagcac tactgctgtt
     3721 atttgctgtt tggcaaatcg taaacaatac tggacttttg cttttagtga tattcggtat
     3781 tttcaatata tatctagcta tgcgcaaaca tcctcgtact aaatttcaaa attttcaatt
     3841 gatattagga agtttggtca ttttttttag cttagtcaac agtcctgctt tatggatgat
     3901 ggctgtattt gccgttttat ttattggtct gaaaggtgta gagatttcag ggatagattt
     3961 aaccaaaaat gctttttggc gaaagaaaca gatcatgatg atccaaacag agcaattaaa
     4021 aacacataat aatgagcgga aaagacagca attattcggt aaccaacgta ttggaaacga
     4081 cgtctatgaa tgggacgata tcaacatcgc cgtcatttct ggtgatacta tcatagattt
     4141 gggcaatacg cttttgccaa aagatgataa tattgtgatt gtaagaaaag ggataggcag
     4201 aacacggatc ttagtacctt tgggcgtagc catccgatta gagcatgcga cattagtagg
     4261 aaatgtcttg tttgaagaag agcaattttc tttgaaaaat gaacaaatca aaatctacag
     4321 cagtgattat gatgaaaacc ctcgtagatt gaagattatt actaatacct tgattggaga
     4381 tgtcgaggtg atacgaatat gatgggaaaa atatccagag cgatgctagc tgtttattcg
     4441 gggattgcgg ctttccttat tattctattt tcacttttca cttattttta tgccagcaat
     4501 caaagtcatt ggtggggaga attactgcgt gcacgtttat tatatgttcc gcttatcttc
     4561 catttgttag ccatttcctt aggtgtagga ttaattgtct ttctattatt atcacttatt
     4621 caaaagacaa aatacgggaa aatcgaagaa aagctgcgtg cactttcttc tggcaattat
     4681 gaatccaaat tgttgcttct tccgatccca agtgcatcgg acgatttata catcaaagat
     4741 attgataagg aaatcactaa gataaaagaa aaaatgattg aagtatctag tgaattacaa
     4801 attgtaacga gccgtcccca atatgtagat ggacaaacca aagaagaaat tttagaatta
     4861 gaaagacatc gattagcccg tgagctgcat gattcagttt cgcaacaatt gtttgcagca
     4921 atgatgatga tgtcggcatt gacggagcaa gcagaaaaaa gcgagacacc agaaatgttt
     4981 cgcaagcagt tgaaaatggt agcagagatc atcaacgctt cccagtctga gatgcgtgcg
     5041 ctgcttcttc atctacgtcc agtcaattta gaagagaaaa gtttaaagca aggtatcgag
     5101 cagttattga aggaattgca aaataaaatc cagatttcgc tgaaatggga tgtagaagat
     5161 gtaaaactat ctagttccat tgaggatcac ttgttccgaa tcgttcaaga attgttatca
     5221 aatacattaa gacatgctaa agccaatgaa ttagaggtat atttgcacaa aatagacaat
     5281 aatcttttgt tacgtattat tgacgacgga acagggttta atatgaatga aacgaaaacg
     5341 ggaagttatg gattgaacaa tatcaaagaa cgagtagctg gtattggtgg aacagtaaaa
     5401 atcattagtt ttaaaggtca aggtacaagt gtagaaatca aggtcccttt gatgaaggag
     5461 gcatagataa ggatgataaa agttttatta gtagatgacc atgaaatggt gcgcttaggc
     5521 gtctcttttt atttatctat tcaagaagac attgaagtaa ttggagaagc tgaaaatgga
     5581 cgacaaggct atgagaaggc gatgacactt cgaccggacg tcattttaat ggacttagtc
     5641 atggaagaaa tggatggtat cgaatcaaca aaagcaatct taaaagattg gccagaagct
     5701 aaaatcatta tcgtaaccag ttttattgat gacgaaaaag tttatcctgc cattgaagca
     5761 ggtgcagcag ggtatttgtt gaagacctct accgcacatg aaattgctga tgctataagg
     5821 gcaacgcaac gtggcgagcg agtattagaa ccagaagtga caacaaaaat gatggaaaaa
     5881 atgagtcggc gaaatgagcc tgtattacat gaagaactaa cgaatcggga aaatgaaatt
     5941 ttaatgctga tttctgaagg aaaaagtaat caggaaatag cggacgaatt gtttattaca
     6001 ctgaaaacag taaaaacaca tgtttccaat atattggcta agctagaagt ggaagaccgt
     6061 acacaagccg ctatctatgc attcaaacat ggtttggtga aataaaaacg atgataaaga
     6121 ggataatcaa aagctgagaa aataactatt atttttcttg gcttttttac aataatgatg
     6181 cttgcttttg tttttgtaaa ggctttgtta tactgagaaa agaagatact aaaggaggaa
     6241 aacggtgaaa caaaattttg caattgtagg tcttggacga tttggcggaa gtatctgccg
     6301 aacactgatc gaagctggtc aagaagtact ggctattgat agtaatgaag atcgggtcaa
     6361 tgaatatatg aatatagcca ctcatgctgt tgtggcaaat gcacaggatg aaatgacact
     6421 tcgatcatta ggcataagaa atttcgatca tgtaattgtc gctattggag aagatattca
     6481 agccagtatc ttagttactc tgatggtaaa ggaaatgggc gtaccaaata ttttagcaaa
     6541 agcccaaaat gagtatcatg ccagagtact tgaaaagatc ggagctgatc gcgtcgtcca
     6601 tcccgagcga gacatgggaa taaggattgc gcataattta gtgtcaaaga atattttaga
     6661 ttacctagag ttgtcagata aatattcttt agcagaaatc cgagtctcca atcctaaatt
     6721 tttcaacaaa acattagctg aattaaattt caggcaacgt tttgacttaa cagtagtcgc
     6781 catacgaaga gtagatcaaa cggttatcgc ttctcctgca gcagatgaat atgttcgtga
     6841 aaatgataat ctactggtta ttggagagac aggcgatgtg gatattctag acgataagat
     6901 gaatcaatag aaagaaaaga gaaagtaggt tgttgggatg aaattaacgg taacgccaaa
     6961 agcacaaaag tggtttgact cagaaattga tctgccagaa acttacggaa tacgtttttt
     7021 tggaaaagtc tacggcaaaa cagaggttca tgatggtttt tcgattggaa tgtcagttga
     7081 acagccagaa catcctgtaa agaaagtgga aattgatggg acactttttt ttatcgagga
     7141 agcagatgaa tggttcttta aaggatatga tttacttgtt gattataatc ccaacctgga
     7201 ggagccaacc tatcacttta aaaaacaata aaaaaagaga gggtgacaca atttttgtca
     7261 ccttctcttt tttcttttga aaatattgtt ttcagctatt aatatcctgc agacaatttt
     7321 acttgattgg tttgaagctc atgggtggca aagtagcttt tcagattggt taaaaaaata
     7381 gccattaatt tcttttgaaa atcaggtgtc aaacctgcaa tgtgggaagt aatcagtaca
     7441 ttgtccattt tccaaagagg atgatcttgt ggaagaggct cttcctcaaa aacgtctaat
     7501 gctgcaaagg aaagttgctg gttatttaaa gcctgcacca gatcttgtgt cttaacagaa
     7561 gcaccacggc caacattgat gaatacagat tttggatcaa agtgttcaaa catatgctga
     7621 tcaaagagcc cagttgtttc ctccgttaga ggtaagatat tgactacgaa atctacttca
     7681 ggaagaactt cctttagttg atttaaagaa aaagtctttt ccattccttc tgctggatga
     7741 ccggatgtat tgattccata gcagttcatg tctaatgctt tgcatttttt tgcaatcatt
     7801 tttccaatat ggccagttcc aatgattagt atttttttcc cttggataga ttggtaatgg
     7861 accgatgaac ctaaccattt catgtcactc tgtgcctttt gtgcttgaaa aagaccacga
     7921 gaatacccaa ggatgattcc taagacatgc tccgtaatcg attcactgtg gatgccacta
     7981 ccatttgaaa gtaaaatatg tttctttgaa aaagtctcaa ggggtagctg atcaacacca
     8041 gctgagatag attggaccca tttcaaggta gaagaggcta atagtttttc ttggtagtct
     8101 gagttccagc ccaaactgat ctctacattt tgtagatcct ctggttgtaa ttctgtttta
     8161 aagatataac caggcgctat agtatggatt tcttcgataa attcatcacg aaatggacga
     8221 tctgcataaa tgattctttc tgacatagtt aatttcccgc ctttctttac tctaagtgta
     8281 gcgcatacaa gaagaacttt gaagcgcctt gcttgttttt tgagaaagaa atatttttca
     8341 aataaaaaag gggctgtgac aaaaaaatgt cacaacctct aaaagggtct attgaagtag
     8401 aaataacgag ttgtctttat ttcttaattg atcgatattt ttgcccatcc tttgacagct
     8461 tgagcaaaaa taaagattcc tgcaaaagca atgaaggcgc tagtgtcaaa agaaggaaga
     8521 aagaccatca agattgttgc aattatagta ggcaatgaaa ttgaatagat caatgttttt
     8581 aaacaatcaa gaaaagtagc ttttgttagt cgaaagcgtg catagagaaa agcagcaatg
     8641 ttggtaaata gcaaagtgat cagcagattc agaaaactag gataaataga aaatataaat
     8701 gtcaggactt ttatccaaag tggtatagat gcttcactta aaaaagaacg gagctgctta
     8761 cctgttaagt tttttaaaga ttcattggtg tagggaagtt ctaattggtt attgttcaac
     8821 aaagcagaag tcgtgcctgt attaggaaga gctatgataa gtttgttttt taacatcccg
     8881 acactaacaa aattccccat caaatcagta gagatgtcct gttctgttcg ttttccctct
     8941 ggatcaaatg tgaagataat ggagtttgtt tggtaaatga atccttcttt ttcttcggtt
     9001 tgaagctgcc cgttttggat tgtaaaatca ggaatgcgag tagcaatctt ttgcccatcc
     9061 aattgaatgt tgtttaagac ttgaaaaact tgaaaagaga ttggcaaagc cataattata
     9121 ctcaaaacta aaagatagac aatggctttc caaaatggga catttctcgc ctcctttagg
     9181 tctttgaatc gtataaagga actgataatt aattgttttg ttgtcataag tactcctcat
     9241 ctagtagtgt catcacttgc aattctagcg aagtatcaag tgaaatacaa gtgctttaag
     9301 gaaacagctg aatgattggt gaaatatcaa aaaaatgtta ttcttgtcta aagactatcc
     9361 aatataaata aaatggagga aggaatagtg cctacacagc cacagagatc atcaaaaaaa
     9421 ctatttggag tagtggtttt gaatgcggtg atcactttat cagaatttat tggagggatt
     9481 atgtcaggga gtttgtctct tgtttcagat gctttccata accttagtga tacattggca
     9541 attatttttt cttacggggc acaaaaaatg gcccgaaaag aagcaaataa aaaacgtacg
     9601 tatggctacc agcgtttaga aatattgagt gctttcatca actcattcat attgatcata
     9661 ttatccttgt ttttaaccgt tgaagcattc aagcggttca cttcaccaga aaagataaat
     9721 agtcatttga tgctaacagt agcagttatt ggattgctcg cgaatttgtt ctcagtatta
     9781 ttattacgac aagaagcaga tgaaagtttg aatataaaat ctagttattt gcatttgtta
     9841 agtgatacac tgtcttcggt aagtgtgatt atcggagcaa ttttgatccg ttttttcgat
     9901 atctactgga tcgatccagt tattacactg atcatttcga tatatatatt gatagaagcg
     9961 ataatggtga tcaaaaaagc tgccgctatt ttgatacaaa gtgcacctgc tatcgactat
    10021 gaaaaaatgg aacaagaaat tgaagcaatc gatggggtaa aggatgttca tcatgtccat
    10081 atttggcagt atagcgaaaa gataattatt ttcgacggac atattgattt tgaagatcag
    10141 ctactaagcg agattgaaaa gatatatcct cgtataattt ctttattaaa attaaaatat
    10201 ggtattacac atgtgacgat ccaagcagag acaaaggcaa aggatcaaaa aaaattaatt
    10261 tttttaaata aagatgaaag ttattgaaat atagagtatg ataaataaga atttttaata
    10321 aggtaggttg gctcatgaag cagtatcgac agttaaaagg atatttagaa gcaaaaggat
    10381 attgggagat actagtctat ctcttttttg gtggtttggc tacgattgta aattttgtta
    10441 gttttgctct cgcacgccaa tattttgatt taagtatggc cttatccaat tctatttcgt
    10501 ggttttgttc tgtattgttt gcttttgtaa cgaacaaact ttgggtattc cattcaaaat
    10561 caccgaattt tacacatgca cttattgaat gcggtaagtt cttcttctac cgtatccttt
    10621 cttacggatt ggacatgggg gcaatggtat tgttgatcaa tgttatgaac agcaatgagt
    10681 atgtggcgaa aatcatcact caaattattg ttatccttgc gaactatatt ttcagcaagt
    10741 tgtttatttt taaagaaact gaggtttttg aagaagaagt aggtcaaagt gaaaaagacg
    10801 actaggcata acaattgaag aatttccttc tctttgttac tctttgtaat gtagacatta
    10861 ttaagaaaac attgaggagg aattttttta tgacttacac attaccagat ctaccttatg
    10921 catatgacgc attggaacct tatattgacg aagaaacgat gcatctgcat catgataagc
    10981 atcacaatac ttatgtgacg aatttaaatt cagcaattga gaaataccca gaattaggcg
    11041 aaaaaacaat agaagaatta ttatctgata tggacgctat tccaacagat atcaagacag
    11101 ctgtacgtaa caatggtggc ggacatgcta accattcatt tttctgggaa atcatggcac
    11161 caaatgctgg tggcgaacct acaggagaaa taaaagaagc gattaatgaa gcttttggtg
    11221 atttttcttc ttttaaagaa gaattcaaaa aagcagccgc tggacgattt ggttctggat
    11281 gggcttggct tgtaatggaa aatggaaaat tagctattac ctctactgca aatcaagatt
    11341 ctccattgat ggaaggaaag acaccaattc taggtttgga cgtttgggaa catgcttatt
    11401 atttgaaata taaaaatgtc cgaccagatt atattgcagc attctggaat gttattaatt
    11461 gggatgaagt aaacaaacgt ttcgaagcag caaaataacc atttcctccg tcatatcagt
    11521 cttaaaaata atttggaaaa taaaagagcg aaagcttgct agcgttttct aaattgggta
    11581 taatgttctt gcaaggttac ttgcaagaac ttttttggag gatatacatt tatggaacac
    11641 ggaacggtta aatggttcaa caatgaaaaa ggatttgggt ttattacagt agacggcgga
    11701 gatgacgtat ttgttcattt ctcagcaatt caaggagacg gcttcaagag cctagaagaa
    11761 ggtcaagaag tagaattcac gattgttgaa ggtgcacgcg gacctcaagc tgctgaagtg
    11821 actaaattat aataatttgt tgaataaaga cacgacttct tagagagtcg tgtctttttg
    11881 tctatttatg aagtttttag attttttggc taagtaaatg catttgttca cttgacatgt
    11941 cctaactaat tatcaagcaa ggaacatttt cttcgttagt aatggatttt aaagaaattt
    12001 cagtttttgg atgttgaata aaagaccatt tgtgaaacca ctagtataca tactcattac
    12061 catgtcaatt ctattacttt aaaagaaaat cgttttactt tgaagaggag catatctatc
    12121 cattaaaaga tgatggtaat gcaatcacta gttcgagtca ttttaagagc catgcagtga
    12181 gctggaaata aaacgaatag aagatgggtt taacaaaagg accagtcata ggaagaaatt
    12241 gtcgtgaaat cgaatgataa tctgaacggt aggcaaagac tgttaaaaag atgggaaagg
    12301 tatgtgcgat cattttactg cacgattatt tggaaaatac cttgttgttc gctttttctg
    12361 gaaaaaatac ataaaaaaag ataaaacgat tgcgaactgc gtgtattcgg tctttcgatc
    12421 tttttcacgg tatggtatg
//
LOCUS       DAYYAG010000082        12177 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000082 DAYYAG010000000
VERSION     DAYYAG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.082"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHARQ_001972"
     CDS             <1..610
                     /locus_tag="ACHARQ_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8410375.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHARQ_001973"
     CDS             723..1001
                     /locus_tag="ACHARQ_001973"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8410376.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHARQ_001974"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHARQ_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8410377.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHARQ_001975"
     CDS             1458..2213
                     /locus_tag="ACHARQ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8410378.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHARQ_001976"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHARQ_001976"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8410379.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHARQ_001977"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHARQ_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8410380.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHARQ_001978"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHARQ_001978"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8410381.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHARQ_001979"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHARQ_001979"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8410382.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHARQ_001980"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHARQ_001980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8410383.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHARQ_001981"
     CDS             6950..7492
                     /locus_tag="ACHARQ_001981"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8410384.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHARQ_001982"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHARQ_001982"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8410385.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHARQ_001983"
     CDS             9085..9987
                     /locus_tag="ACHARQ_001983"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8410386.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHARQ_001984"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHARQ_001984"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8410387.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHARQ_001985"
     CDS             11430..11852
                     /locus_tag="ACHARQ_001985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8410388.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4048 a         2159 c         2688 g         3282 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagagaaa tcttaga
//
LOCUS       DAYYAG010000083        12118 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000083 DAYYAG010000000
VERSION     DAYYAG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.083"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..565)
                     /gene="frr"
                     /locus_tag="ACHARQ_001986"
     CDS             complement(8..565)
                     /gene="frr"
                     /locus_tag="ACHARQ_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8410389.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(568..1290)
                     /gene="pyrH"
                     /locus_tag="ACHARQ_001987"
     CDS             complement(568..1290)
                     /gene="pyrH"
                     /locus_tag="ACHARQ_001987"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8410390.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1426..2307)
                     /gene="tsf"
                     /locus_tag="ACHARQ_001988"
     CDS             complement(1426..2307)
                     /gene="tsf"
                     /locus_tag="ACHARQ_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8410391.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2406..3188)
                     /gene="rpsB"
                     /locus_tag="ACHARQ_001989"
     CDS             complement(2406..3188)
                     /gene="rpsB"
                     /locus_tag="ACHARQ_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8410392.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3547..4026
                     /locus_tag="ACHARQ_001990"
     CDS             3547..4026
                     /locus_tag="ACHARQ_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8410393.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4243..5334
                     /gene="phnW"
                     /locus_tag="ACHARQ_001991"
                     /pseudo
     CDS             4243..5334
                     /gene="phnW"
                     /locus_tag="ACHARQ_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5327..6112
                     /gene="phnX"
                     /locus_tag="ACHARQ_001992"
     CDS             5327..6112
                     /gene="phnX"
                     /locus_tag="ACHARQ_001992"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8410394.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6458..8149)
                     /gene="argS"
                     /locus_tag="ACHARQ_001993"
     CDS             complement(6458..8149)
                     /gene="argS"
                     /locus_tag="ACHARQ_001993"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8410395.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8571..9518)
                     /gene="arcC"
                     /locus_tag="ACHARQ_001994"
     CDS             complement(8571..9518)
                     /gene="arcC"
                     /locus_tag="ACHARQ_001994"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8410396.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9633..10652)
                     /gene="argF"
                     /locus_tag="ACHARQ_001995"
     CDS             complement(9633..10652)
                     /gene="argF"
                     /locus_tag="ACHARQ_001995"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8410397.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10743..11972)
                     /gene="arcA"
                     /locus_tag="ACHARQ_001996"
     CDS             complement(10743..11972)
                     /gene="arcA"
                     /locus_tag="ACHARQ_001996"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8410398.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3317 a         2526 c         2180 g         4095 t
ORIGIN      
        1 gattttatta aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt
       61 taatgctgtt atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg
      121 taatatcacc atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac
      181 gaatcgcgat ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc
      241 gttcttctgt cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga
      301 ttccgatatc acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag
      361 gtgtgatcat taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg
      421 ttggcgcacc gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg
      481 cgcggatttg cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg
      541 cttctgtcat aattgcatct gccattttta tttccccctt acagttgttc cgatattttc
      601 gcctaagatt gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat
      661 atcattatcc atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat
      721 aacttccaga tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc
      781 agaataaaca ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc
      841 cgctcgtaat gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa
      901 gataacgatc cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc
      961 gatttgacgc atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt
     1021 atcctgtaaa gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc
     1081 acgttccatg cccatttgtg ctccgatttg tccgcgccag atatttccgc cacccacaac
     1141 gattgccatt tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat
     1201 tactggtggt ttgattccaa atcctgcatc cccagctaaa gcttctccac tcaattttaa
     1261 gactacacgc tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat
     1321 tttaccatac ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa
     1381 aagggagcgc acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg
     1441 gctcataact tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa
     1501 acgtacgaat gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc
     1561 aggatcttta acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg
     1621 tccttctacc attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt
     1681 aagaacagtt ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg
     1741 aggattgata gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc
     1801 atctagaaca gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc
     1861 attgtcgtct ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg
     1921 agttgcttcg atcaagtcat attcaattgt acctttatct gttttgattt tcattgcagc
     1981 ttccatgtct gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg
     2041 gaacatttcg tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt
     2101 gttacctttc acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc
     2161 agcctttgcc attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac
     2221 ttcaactaat gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt
     2281 taccatttta gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa
     2341 aaaagctgct tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt
     2401 caaaattatt ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat
     2461 gttgctgtgt tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct
     2521 tcgatgaaag catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg
     2581 tttgaaggga ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg
     2641 attgggatat ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg
     2701 atgtacatta catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga
     2761 cgttcacgtt ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca
     2821 tcttcttcca ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag
     2881 tttgtcaatg ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct
     2941 tcttctttga ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct
     3001 tcttcagcaa cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc
     3061 aagtcaatga tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc
     3121 cagcgacgag tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt
     3181 actgccatgt ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg
     3241 ccggcaaact ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca
     3301 taaagcaccc tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt
     3361 tctgctcaac attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat
     3421 ttctctttgt ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat
     3481 attattgggc aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga
     3541 aagaaaatgc gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg
     3601 atccgtacgc aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag
     3661 gcaacgattt ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc
     3721 gtttcccgct tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag
     3781 gatcggctgg ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg
     3841 acgatcgtca ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc
     3901 aaacctccct ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa
     3961 aatgaggagg acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt
     4021 ttttaatcag agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc
     4081 cttattaaat aaacctttac actctttaca aaaatacatc attttttatt caattttttc
     4141 aacaccttga ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc
     4201 ttgctctttg tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact
     4261 cctggaccat tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact
     4321 tgggatcaag aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct
     4381 caagtatcgg aaggaatata cacaacggtc ttaatgcaag gtagcggctc ctttggggta
     4441 gaatcggtat taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc
     4501 gcatatggac aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat
     4561 gaggtatcgt aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat
     4621 ccctctatct ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat
     4681 ttggaagaga ttgctgctgt cacacaagaa aaagatcttt tgtttatagt ggacgcgatg
     4741 tcaagctttg gtgggatacc gattcgtgta gccgaactag ggattgattt tttgatttcc
     4801 agctcaaaca aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg
     4861 gaactagaaa agcgaaaaaa tgtgagtcga agtttgtcac ttgatctata tgaccagtac
     4921 cgcgtgatgg aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg
     4981 gcttttcaac aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca
     5041 cgttatctcg ccaataatca tgtactacga caaaaaatga cagagatggg gttccagcct
     5101 ttcattcaag aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga
     5161 tttgatttta atcattttta tcagtattta aagcaaaacg gatttgtgat ctatcccggg
     5221 aaaatcagcg atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat
     5281 attttggaac tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt
     5341 aatttttgat tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt
     5401 tagaaatgct tttctagaaa aaggaatcca actgactgac aaagaaatcc gcgaaccgat
     5461 gggaaaatta aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca
     5521 atggatagct atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca
     5581 atttgaacat tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt
     5641 ggaaacgatc gaacggttga gaaacgctgg gatcaatatt ggttccacga ctggatttac
     5701 tgctcctatg atgacaatcg tagcagaaac cgcaaaaaaa gaagggtatt caccagactt
     5761 catggcaact tctgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa
     5821 aaacatgcag tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt
     5881 ctcagatatc caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag
     5941 ttcgctaatg gggatttctt atgatgagta ccaaaaatta gagatgggga aaaaagatcg
     6001 tttaaaacag tctgttgctc ttagattcca acaagctggc gctgatattt gtgtaaacaa
     6061 tttaaaagag ttaacagaat acattttgta cgctgacaag aatccagaat gacttttata
     6121 caaaatcaga ggtcgtgaaa aagctgtact gcatcaagta ataagaacag ccaaacaaaa
     6181 atagctcctc atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca
     6241 ccgtttattc gaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttattta
     6301 caaacaaaca gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct
     6361 cggtatttgc tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat
     6421 actcggatca gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa
     6481 gtcctagcaa acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc
     6541 gtgcttcttt ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg
     6601 cttgtgctaa tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag
     6661 acaagaccgt ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat
     6721 tcaatgcata gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg
     6781 cacgagcatg tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt
     6841 ccaggttaaa gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc
     6901 caaccccaac ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga
     6961 tttgttcttt agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc
     7021 ctttacgagt tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggcgca
     7081 tatcgtcaga ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat
     7141 agctttgttc attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt
     7201 acaatgcagc agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca
     7261 acgctggatt caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca
     7321 gcaaatgttt ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt
     7381 caccgttcaa cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca
     7441 tcgattcatc acggaaccat tgccataatt gaatagcctc ttcatcgcct tcttccagac
     7501 gtttgaacca agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga
     7561 attggacata taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac
     7621 cccatttttt gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt
     7681 tgatgcggat tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa
     7741 ctgtcgaacg caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga
     7801 ttggtactgt tccttgattc ccgatattgc tatccccata atgttctttt tctttcacta
     7861 ctgtttgcaa aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc
     7921 cgacaacttc gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga
     7981 tttgctgtgg cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc
     8041 cgtgttcagc tgattttgga ttttctagta attgttcgac ttgttccagt gttaaatcat
     8101 ctttgacagc gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc
     8161 ctaaagtgct cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg
     8221 ccatcactag agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc
     8281 tccatctgat atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt
     8341 tgtatcttct tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt
     8401 actctcttgt tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa
     8461 aacgaaaggc tagtcgtttc tgataaaaaa acaatccaga tggcctaaag gagaagaaat
     8521 accacctgga tagctataaa tcatttcgta aatacgaaag acttaacaaa ttaatcagct
     8581 acgataatcg ttccttcttc agaagctaaa agattctcga tattttctaa agaagtgatg
     8641 attgccttag cgtttggttt tgcttcaaca aatgcaatag ctgcctctac tttcggaagc
     8701 atgcttcctg gagcaaattg cttttcatca atgtattgtt tcatttctga aacagtaaca
     8761 gtttctaatt tcttttgatc tggtttttgg taattgacat acacattgtc tactcctgtc
     8821 aatacgatca gcaaatccgc atcgatgatt tcggctaatt tttctgaagc aaaatcttta
     8881 tcgattacgg cttctcgtcc ttctaatcca gtagctgttt ctacaacagg aatcccaccg
     8941 ccaccaacag agactgtgat aactccttga tcgaccagcg tttcgatgac acgtgcttct
     9001 ttaatcgaga tgggtttagg ggaagcgact acttttctcc atccacgtcc agcatcttct
     9061 ttgaatgtca cagtggagtc agcattcatt tgttctttgg cttcttcttc cgtgtagaat
     9121 ggaccgactg gtttacttgg atttttgaat gaaggatcat tttcatccac aataacttga
     9181 gtgactaaag aaaccacatc tttatcgatc cctttttcct tcagtacttc gcccatggca
     9241 ttttgtaacc agtaaccgat agatccttct gtcattgcaa cacatgtatc taaaggcatt
     9301 gccggtgttt tctcagaatc agcagcttgc tgctgaatca ataggttacc gacttgcgga
     9361 ccatttccgt gggagatgat caattcatct ccttgttcga taaatttcac taaatacttt
     9421 gctgtttcca ttaacgcttc ttgttgggct tttgcacttg catccgtaga caagattgca
     9481 ttacccccta aagcgacaac tacttttcgt ttggccattt cattcgtcct ttcttcagtt
     9541 ctaccgaatt ttctagataa caaaacgagc cagcgttttg tgattttccc tcaatgctga
     9601 ctcgtttttt cgtgagatga atgtggttat gcttaaacgc gagggataaa caagttacct
     9661 aatgttgctg ccatgatcgc tttgattgag tgcatacgat tttctgcttg gtcaaattga
     9721 cgagcatatt tgctgcggaa gacttcatct gttacttcca tttcagtgat gccaaagcgt
     9781 tctttcattt gttcaccgta aacagtgttt gtatcatgga aagctggcaa gcagtgcaag
     9841 aagatcaaac gatctgtgtt gtgtgttttt tctaccattt ccatattgat ttggtaaggt
     9901 ttcaacaatt tgatacgttc ttcgaattta tcttcttcac ccattgatac ccatacatca
     9961 gaataaagga cgtctgctcc gtctacacct ttatcaacat cgtcggtaat catcaattgt
    10021 gcaccggatt tttcagcaaa gccttctgcc attttgacga tttcttcttc aggttgtaat
    10081 tcttttggtg caacgatacg catattcgct cctaaaattg cacctgtgac taataaagag
    10141 ttagccatat tgtttcgacc atctccgcag taagcaacag tgataccttc tactgttcca
    10201 aagttttctt ggatagttaa aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc
    10261 aagccattcc aaactggaac accagagaat tctgctagtt cctctaccat tttttgactg
    10321 aatccacgga attcgattcc atcaaacata cgacctagta ctttagctgt atcttctgtg
    10381 gattcttttt tacctaattg gatatcattt gcacctagat attcaggatg tgcacctaga
    10441 tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag atgttttttc aaataaaagc
    10501 gcaatatttt ttccttctag atagtgatgt gggataccac gttttttcaa gtctttcaag
    10561 tgttctgaaa aatcgatcaa atattgtaat tcttcttttg taaagtcttt ttctgctaat
    10621 aagcttcttc cttggaatac tgattctttc atattaaaca ttctccttta gattgttttt
    10681 atttaagatt aaagaggaaa tcccgacggc agctgacagt aagaccgccg tcaggaaaca
    10741 aattacttca agtcttcacg gacaagtggt tggctcatgc agcgtggacc accacggcct
    10801 cgtgacaatt cacttgaatt gatttctaac actttgatgc catagctacg aagcaattcg
    10861 ttcgatacat aatttcggtt ataagtgact acaacacctg gagcgattgc caatgtgttt
    10921 gatccatcat tccattgttc tcgaggtgca acgatctcat ctccattacc agttgggatc
    10981 agtacaagat catctaatcc aagagcagct ttcatcgttt cgtgtaagtt atctgaatga
    11041 gtaatcttga tatcatcacc atctggttcg attgtaaaga catccatctt accgtcttta
    11101 ctttgaattg ctggatggat agtgaattta tcgtgatcta ccattgtaaa tacggtatcc
    11161 aaatgcatca ttgcacggtt gtttggaatt ttgattgcta acactttttc aaatcctgaa
    11221 ttttttgcga ataggtggcg tgctaatttt tctaaagctt tcgcattagt tcgttgagaa
    11281 atcccaacag ctactacttt atcgcttaag atcaattcgt ctccgccttc gatatgatct
    11341 ggattttcac gatccaacca tacttcgatt cctttatttg caaatcttgg atgatgtttc
    11401 aaaatatatt ctgtaaacat cgattcacga cgacgtgctt caaaagtcat cttattgact
    11461 gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg
    11521 tccatgtaaa atggatagtc atcgtctgct gacagatcgt atagactgct agaacgtact
    11581 tcaatatctt ttgtacgaac cccagccatg attttatcta ccatttcttg cgtttccatt
    11641 gatagaagga actcatgaag accctctctt acagcattcg atgcaatatg agattcatcc
    11701 aacattttat tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat
    11761 ttttccaagt aaagagtctc gacgccttca ttttgcaatg tctttgcaaa gttgtcatgt
    11821 tcttcttgag caatcggaag atatggaata tcatcgaaca acagacgatc cataatatct
    11881 ggtgtcaaat tttccacttc ttgtccaggc cttttcagta aaactgtctt taatttccct
    11941 atttcagaga aaacgtgaat aggtttatcc atgttgtgtt gcctccttct ttttgattac
    12001 atgtatagga tatcctcctt ttgtaatcac tttcataatt ttgtctcatt caaaatgaga
    12061 cattcctcac attaatattc gataattaaa taattaaacg tttttctatt tttaagcg
//
LOCUS       DAYYAG010000084        11908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000084 DAYYAG010000000
VERSION     DAYYAG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.084"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHARQ_001997"
     CDS             <1..465
                     /locus_tag="ACHARQ_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8410399.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHARQ_001998"
     CDS             complement(1080..2120)
                     /locus_tag="ACHARQ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8410400.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHARQ_001999"
     CDS             complement(2469..2795)
                     /locus_tag="ACHARQ_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410401.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHARQ_002000"
     CDS             complement(2782..3570)
                     /locus_tag="ACHARQ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8410402.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="ACHARQ_002001"
     CDS             complement(3938..4228)
                     /locus_tag="ACHARQ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8410403.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="ACHARQ_002002"
     CDS             complement(4364..4543)
                     /locus_tag="ACHARQ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410404.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="ACHARQ_002003"
     CDS             complement(4540..4890)
                     /locus_tag="ACHARQ_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410405.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="ACHARQ_002004"
     CDS             complement(4883..6208)
                     /locus_tag="ACHARQ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8410406.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="ACHARQ_002005"
     CDS             6658..7272
                     /locus_tag="ACHARQ_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8410407.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="ACHARQ_002006"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="ACHARQ_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="ACHARQ_002007"
     CDS             complement(7883..9202)
                     /locus_tag="ACHARQ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8410408.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9336..9944)
                     /locus_tag="ACHARQ_002008"
     CDS             complement(9336..9944)
                     /locus_tag="ACHARQ_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410409.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10582..10839)
                     /locus_tag="ACHARQ_002009"
     CDS             complement(10582..10839)
                     /locus_tag="ACHARQ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8410410.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10832..11101)
                     /locus_tag="ACHARQ_002010"
     CDS             complement(10832..11101)
                     /locus_tag="ACHARQ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8410411.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11232..11411)
                     /locus_tag="ACHARQ_002011"
     CDS             complement(11232..11411)
                     /locus_tag="ACHARQ_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410412.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11509..11745
                     /locus_tag="ACHARQ_002012"
     CDS             11509..11745
                     /locus_tag="ACHARQ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410413.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3675 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
    10321 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
    10381 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
    10441 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
    10501 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
    10561 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
    10621 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
    10681 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
    10741 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
    10801 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
    10861 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
    10921 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
    10981 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
    11041 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
    11101 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
    11161 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
    11221 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
    11281 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
    11341 tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt
    11401 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
    11461 atcatcttga atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat
    11521 accagtattg ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt
    11581 atttcgccaa caaataggac taacaataga ggaatgattt tattcatatg gatctccttc
    11641 tttctcttag gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg
    11701 ttggagcgat tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat
    11761 ttcacacatt ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt
    11821 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat
    11881 aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DAYYAG010000085        11867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000085 DAYYAG010000000
VERSION     DAYYAG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.085"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..233
                     /locus_tag="ACHARQ_002013"
     CDS             <1..233
                     /locus_tag="ACHARQ_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410414.1"
                     /translation="SKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSL
                     TPDEKSKCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            299..1627
                     /locus_tag="ACHARQ_002014"
     CDS             299..1627
                     /locus_tag="ACHARQ_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8410415.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            1527..2282
                     /locus_tag="ACHARQ_002015"
     CDS             1527..2282
                     /locus_tag="ACHARQ_002015"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8410416.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            2286..3497
                     /locus_tag="ACHARQ_002016"
     CDS             2286..3497
                     /locus_tag="ACHARQ_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8410417.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            3508..3819
                     /locus_tag="ACHARQ_002017"
     CDS             3508..3819
                     /locus_tag="ACHARQ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8410418.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            3816..4154
                     /locus_tag="ACHARQ_002018"
     CDS             3816..4154
                     /locus_tag="ACHARQ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8410419.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            4147..4578
                     /locus_tag="ACHARQ_002019"
     CDS             4147..4578
                     /locus_tag="ACHARQ_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8410420.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            4575..4916
                     /locus_tag="ACHARQ_002020"
     CDS             4575..4916
                     /locus_tag="ACHARQ_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410421.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            4919..5509
                     /locus_tag="ACHARQ_002021"
     CDS             4919..5509
                     /locus_tag="ACHARQ_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8410422.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            5519..5899
                     /locus_tag="ACHARQ_002022"
     CDS             5519..5899
                     /locus_tag="ACHARQ_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410423.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            6097..8667
                     /locus_tag="ACHARQ_002023"
     CDS             6097..8667
                     /locus_tag="ACHARQ_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8410424.1"
                     /translation="MIYLADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSE
                     FDKNDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLN
                     NAKAQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKF
                     GGVLKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNA
                     DNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDI
                     ESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEA
                     IHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQF
                     QNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTL
                     IPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNG
                     LIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQ
                     LLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIIT
                     TIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLA
                     NAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPST
                     LFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSM
                     LDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY
                     "
     gene            8738..9139
                     /locus_tag="ACHARQ_002024"
     CDS             8738..9139
                     /locus_tag="ACHARQ_002024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410425.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            9216..9908
                     /locus_tag="ACHARQ_002025"
     CDS             9216..9908
                     /locus_tag="ACHARQ_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8410426.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            9905..>11867
                     /locus_tag="ACHARQ_002026"
     CDS             9905..>11867
                     /locus_tag="ACHARQ_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8410427.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRI"
BASE COUNT         3682 a         2218 c         2777 g         3190 t
ORIGIN      
        1 tctctaaatt caaagattgg aaaagtttta tagaactaaa ttatttacaa gatttcttcg
       61 atgatgaaga ttatatgacg tttaaaaaac ttgcaccaag tagttcattc tttccttatc
      121 aaagaaaatc cttaacacct gacgaaaaat cgaaatgttt agaggaagta aatacctttt
      181 taaataatgc aattgagata attttgtcaa gaggaagtcg gttttatatc taatgtgaac
      241 acagcatcta caaatcgtag gtgctttttt gatactcaaa ttttgaaagg agcaatcaat
      301 gggcataata aaatcactat tcaaatcacg cgataaacca acaaatcaaa ccatcatgaa
      361 tccataccga tttatgtttg gcggctcatc agctggtaaa agcgtgaccg aacaaacagc
      421 tatgcagatg acggctgtgt actcatgtgt gcgcgtgcta tcagaagccg tggcaggatt
      481 gccattacac ttttaccaat accgagatgc tggcggcaaa gataaagcaa tgaaacattc
      541 gttgtatttc ttgctgcatg atgaaccaaa ttcggaaatg acatcatttg tatttcgtga
      601 aaccttaatg actcacctgc tactttgggg taacgcctat gcgcaaatta tccgaaatgg
      661 caaaggtgaa gttgtagctt tgtatccttt aatgccagat aagatgacgg ttaatcgcga
      721 tgacaacaag gaaatctatt atttatatca agctgatgtc gggcaagtca gactttcaaa
      781 agctgacgtg cttcacattc ctggccttgg ctttgatggc ttggttggtt attcaccaat
      841 tgccatggct aagaatgcta tcgggcttgc gattgcaaca gaggaatatg gttctaaatt
      901 ttttgccaat ggtgccaatc ctggtggtgt cctagaacat cctggaactt tgaaagaccc
      961 aggaaagctt cgtgaaagtt ggaatgcgac atttggtggc agttcaaatg cggccaaggt
     1021 tgcagtactt gaagaaggca tgaagtatac gcctatttcc atgtcaccag aacaagccca
     1081 gttccttgaa acacgtaagt ttcaactgga agaaatcgca cggatattcc gagtgcccct
     1141 tcacatggtt ggcgacttag accacgcgac cttctcaaac gttgaacatt tgagtttgga
     1201 ttttgtgaag ttctcattat caccatggct caatcgttgg gaacaagcac ttaataaatc
     1261 actgttaagt cgcgatgaaa aagaaaagta tttcataaag tttaatgtgg atggcttatt
     1321 gcgtggtgac tatgccacac gtcaacaagg ctatgcaacc ggtattcaaa atgggttcct
     1381 atctcctgat gatgtgcgtg aacttgagga cttgaaccca atccctggtg gtgctggtgc
     1441 gaaatattac ttcaatggca atatgctgcc aattgaacaa gccggggcag cttatggcgt
     1501 aaacaaagaa gaaagcgagg aacaagatga agaagttttg gaagtggaag aatcaaacgg
     1561 ttctgaatca agacaaccaa gaggaaatcc tagagagaac cctctacctc aacggaccaa
     1621 tcgctgagga gagctggttt gatgatgaag tcacaccgca gattttcaaa gatgagttaa
     1681 attcaggtgg aggtaacgtt acagtctgga ttaattcggg tggtggcgat tgtgtcgctg
     1741 ccgcaagtat ttacaacatg cttgctgact acaaaggtaa agtcacagtc aagattgacg
     1801 gcatcgccgc ttcggccgca agtgttattg caatggcagg tgacaaggta ctcatgagtc
     1861 ctgtggcgat ggtgatgatt cacaatccgt cagcggttgc gtttggcgaa aaagttgata
     1921 tggagaaagc cattgccatg cttgatgaag tgaaagcaag tattatcaac gcctatgaac
     1981 gtaaaacgaa tttatcgcgt gtgcaactgg cacatatgat ggatgcggag acgtggatgg
     2041 acgcaaataa agctgttcac ctaggatttg ccgatgaaat catcggtgag attgacccgg
     2101 atattccggt taataattcg atgtactcgg aagtcaaagt aactaattca ctgacttcag
     2161 cgattgctaa gaagtgtcgt attgaaccac ctgaaccaaa ggaacgagaa gaaattaata
     2221 acactatccc agccgattcg ttaatgagtc ggcttaattt aatgaaaaat tggaggaact
     2281 aacccatgaa caaaattcaa gaattgatgg aaaaacgtgc acaagcttgg aacggagcaa
     2341 aggcttttgt agaagcaaag caagataaag atggcttgat gtctgaagaa gatgccaaga
     2401 cttatgcgga aatggaaaca aaagtgaaga actttacgat ggaaattgaa cgtatgcagt
     2461 caatggaagc gatggatcgt gaacttgaaa agccaatgag ccaaccatta acttcgcaac
     2521 ctatgactac tgaaaataaa atggaaccta agaaaacagg acgtgcttca gctgaatata
     2581 aagaaggtgt acttgaagcc ttgcgctcaa acttccgaca tgtttcaaat gttcttcaag
     2641 aaggtgtgga tacggcaggc ggttatttgg tgccagaaga atatgactca cgtttgattg
     2701 acggcatgac ttctgaaaac atcatgcgta cacttgcgac aacaattacg acaagtggtg
     2761 aacacaagat taatattgca ggttctaaac cagccgcttc atggattgaa gaaggtggcg
     2821 agttgagctt tggggatgca acgttcgatc aaatcttgct tgatgctcac aaacttcatg
     2881 tggcaatcaa ggtgactgat gaattgcttt atgacaacgc ctttaacctt gaaaactata
     2941 tcatcgacca attcaccaaa gccttagcaa acgcggaaga agatgcgttc ttgaatggtg
     3001 acggtgttgg taaaccactc ggtatttttg cagcaacagg tggcggacaa gttgccatta
     3061 caactaatac tcaaagtgca gttacggcag atgatgtggt gaaccttgtg tatgctttga
     3121 aacgtccata tcgtaaaaac gcgaagttta tcatgaacga ccaaaccatc agcttgcttc
     3181 gtaagttgaa agacaacaat ggtgcctaca tgtggcagcc agctttggta gcaggtgaac
     3241 ctgaccgtct gctcggttat ccagtttata cttcggagtt tgttccaacc gttaaggcag
     3301 gtgcgccagt cattgctttt ggtgatttca gctactataa cattggcgac cgtggcgtgc
     3361 gttcattcga ccagttgcgt gaactctttg caggtaatgg catggttggt ttcttagcta
     3421 aagaacgtgt ggacggtaaa cttgtgttac ctgaagcagt gcaaatcttg aagatgaaag
     3481 catctgcctc atcttaagga gtagcctatg agtgacttac taaataaggt caaggcaaac
     3541 ctcatacttg agcataacga agatgatgag ctgttggaac gattgattct gaccgctctt
     3601 tcttatgctg agagctatca acatattgag tctggctact attcagaaca tgaaatgccg
     3661 ccaaccactg aacaggcggt tattgtgtta gccagtaact tttacgaaag ccgtgatggt
     3721 tcaactggtg gcttctttgc ggataacgtc aatgccagta agcaagtctg ggaaacggtc
     3781 aatctgttac tcaggctcga taggcggtgg caagtatgag ttttggaaag atgaatcaac
     3841 gaatacaaat cgttaaagtg acgaaccaaa aggatagcgc tggattttca gtgaaacaag
     3901 atgaagttct tgcagctgtc catgcataca aagaggaaaa gaatgcgact gagaaatggg
     3961 taaacatggc gacttttagt tcagcgacct gcctgtttcg gattcgttat attcctaatc
     4021 tagaggttac gacagagatg gttgtcattt cagatcagaa aaggtatcag attgtgtcgg
     4081 tggagaatgt ccgaaatcgc aatatgtacc ttgaaatgct gactactgaa atggaggtga
     4141 gtgcggatgg ctaaagcatt gatgaagatg ccagaagatt tcttgttaaa ggtttcaaaa
     4201 ctagaaaaca agacagatga aatcatcccc aaggttttag aaagtggtgc tgaagtggtg
     4261 gaaggcaaag tccgaagtaa cttaactgcc gttgttggct caaatacaaa agtaccaagt
     4321 cgttcaactg gtgaacttga atcagccctt ggtatcacac aagctcgtca agataaaagc
     4381 ggcaactgga acatcaaggt tggttttgat gagcctcgaa tcgatggcag ttcaaatgct
     4441 aagattgcga acattctcga atatggaaaa cacggtcagc cagcaaagcc attcttgaaa
     4501 cctgcaaaat cacaaagtcg caagtcatgc attgaaacaa tgaaatcaaa aatggaaagt
     4561 gaggtaaacg gcatttgaac attttagagg aattgaacag tctttttgcc acacttcata
     4621 ttcctgttga aacgggcgag tttagtgata aagcgccaga caagtattgt gtgctgacac
     4681 cactcgctga caggttcgat gtttacgggg ataactttcc aaccgttgat gtacaggaag
     4741 ttcgcatttc cttgtttgtg aaaggcaact atctgaacac gcaaaaacaa ctggttcaga
     4801 acttgttgaa acacgagttt acgattactg aacgattatt tgtaggattt gaaaaagata
     4861 caaattacta tcatgtggcg attgatgtcg ccaagcatta tgaaatggag gtctgaaaat
     4921 ggcaacgatt ggattggata aattatatta tgcaccgatt acagagtcgg caactactgg
     4981 cgaagaaaca tacggcacac ccgctatgct tgcgaaagca atctcagctg agctttcggt
     5041 ggaacttgct gaagcaactt tatacgccga tgatggtgca tcagaagtag tgaaagaatt
     5101 taaaaatgga aagttaacgc tcggtgttga tgatattgga cgtgctgtgg cggcagtgct
     5161 tgttggtgca accgtggatc aaaacggtgt cttaatttcg acaaccgaag atggtggcac
     5221 accagttgcg attggtttcc gtgctcgtaa atcgaatggc aaatacaaat atttgtggtt
     5281 gtaccgagtg aagtttgccg tgcccagcac aagcctagca acaaaaggtg acgacattac
     5341 attctcaaca ccaacaattg agggtacagt catgcgtcgt aacaagttag atgctagtgg
     5401 taagcatcca tggaaagcag aagttgatga aaacgatacg ggagttgaac aaacaactat
     5461 caatggatgg tacacgagtg tttatgaacc agatttcaca gcagactagg aggcaacaat
     5521 gagtgaagaa cgcagtgcca agattagcct tggcggagat gaatatgaac tgattttgac
     5581 aacaaaagcc acgaaagaaa tcgccagtca ctacggtggg cttgataacc ttggtgagaa
     5641 attgttgaaa tccgagaact ttgaattggc gcttgatgaa atcatctggt tgattacgct
     5701 gttggcgaat cagtccatca agatttacaa cttgaagaac acagaaaaga aagatgaact
     5761 cacgactgaa tatgtggagt tgatgacgag tccgttgcag ttggctgaat ataaatcagc
     5821 aatcactgaa gcaatgttca agggcacagc tcgcaatatc gaaagtgaaa cagattcaaa
     5881 aaacaaagca ggcgagtaag tgatgaagaa acatttactc gcctgtatta ctatgggaca
     5941 gttcaaatgg gtatgacgtc agatgatttc tggtactgct cgcttggact gtttttggat
     6001 ttatgggagt gtcataagca atttactggg attagcaagc cgaaagttga gttattcatt
     6061 gatgatgtga taccgagtgg aatttaaggg aaggtggtga tttatttggc agataatttt
     6121 ggtttaaaga ttggcgttga aggtgaaaag gagtttaaga actcgttgcg tgaaatcaac
     6181 caatcattca aggtgctagg ttcagaaatg aaactggtat catccgaatt tgataaaaac
     6241 gataaaagca tcagctcact gtctgctcgc aaccaagtct taaacaaatc gattgatgcc
     6301 cagaaagaaa aaatcaccac ccttgaaaat gctttgaaga acgctagtga cagttttggt
     6361 gaaaacgata agcgtactca gaattggaca gtgcaattaa acaatgcaaa agcccaactc
     6421 aataatatgg agcgtgaagt tgctaacaac gagaaggcaa ttgacgccat gggtactgaa
     6481 gaagttgatg ctgctaagaa aactgatgag cttggcaacg aactcaagga tacaggtgac
     6541 gaggctgaaa agtctggaag taaatttgaa aagttcggtg gcgttctaaa aggtatcggg
     6601 gtagcaatgg gtacagtggc acttgctgct ggcgctgctg ccgtggagct aggaaaacaa
     6661 gtggtatctg cttacgcgga ctatgaacaa ttagtcggtg gggtggatac tcttttcaag
     6721 gatgcgtcat ccaccgttca aaagaatgcc gacaatgctt tcaaaactgc cggaatgtca
     6781 gccaatgaat acatggaaac tgtcacaggt ttctcagctg ccttgatttc atctctcggt
     6841 ggtgatacca agaaagccgc aggagttgcg gatcaagctg ttactgatat gagcgataat
     6901 gcaaacaaga tggggacgga tattgagtcc atccagaacg cctatcaagg tttcgccaag
     6961 caaaattacg ggatgctgga caacttgaaa ttaggttttg ggggcacgaa gtccgaaatg
     7021 gaacgtttgc tccaagaggc tgagaagatt tctggtgtca aatatgatat ctcgaacttc
     7081 gctgatgtga ctgaagcgat tcacgttatt caaaccgaaa tgggcatcac tggtacaacc
     7141 gccaaggaag cgagtgagac gataagtggt tcgattgacg gtatgaagtc tgcaatctca
     7201 aacttgatgg ctggacttgg taacagcagc gctgacattg gtatgttgat tggcaatgtg
     7261 gtggaacaat ttcaaaacgt catcaagaat attctaccgg tcattcaaaa tatcgtgtcg
     7321 gctttaccgg cagtggtgga tgccattatt caagcattgg ggcagttatt gccaacactc
     7381 ttagaagcag taacctcatt attctcacaa gtgcttgaaa cgatacttgg cttacttccc
     7441 acacttattc cagtggtggt tcaagccgtt ttaacgattg ttcaaacact tattgagaac
     7501 ttacctttac tgattgacgc tgcctttcaa cttgtgacaa cacttatcca aggactaggc
     7561 gaagcgctgc cagaacttat tccaattaca gtacaggcaa tcataacgat tgtgaacggc
     7621 ttgattgaaa acttgccatt actgttggat gctgctttac aacttatcat gggacttgcc
     7681 caaggactta tcacagcact tccaatactg attgaaagtc tgccacagat tattacagga
     7741 atcgtgaact ttattatcag tgctattcca caaatcattc agactggtat tcaacttttg
     7801 acatcacttg tgacagcttt gccggatatc atcaatgcga ttgttgcagc tattccagtg
     7861 attattcaaa atatcatcaa tgccgtgatt ggggcaatcc ctcagttgat tgacgcaggt
     7921 attcagttac ttgtggcatt gattggtgcg ttaccgcaaa ttatcacaac gattgcgaat
     7981 gccatgccgc aaattatcaa cggaataaca ggaactttgg taaacaacat cgacaaaatc
     8041 atcaatgctg gtgtgaagtt gcttgtggca ttagttcaaa acttaccagc aatcatttct
     8101 gccattgtca aagcagtacc gcaaatcatc acagggcttg cgaatgcttt cggtgagtat
     8161 gtttcaacta tgggaactat cgggaagaac ttgattacag gattgtggaa tggtatcgct
     8221 gatgctggaa gttggttacg caacaagatt tcaggattct ttggcggtgt agtagacaac
     8281 atcaaggact tctttggtat tcattcacca tcaacactgt ttcgtgatga aatcgggaag
     8341 tacatggcac aagggattgg cgtaggtttt gataaggaaa tgactggtgt ttctaaaacg
     8401 atgcagaaag ctattccaac aaaactttat atgccaagct ttgatattga taccgatatc
     8461 cacgctgccg ttgatagcac attctcgatg cttgatttag gtgctaaggt tgatgcctta
     8521 acaaacatca cgagtgaaat gttgccaacg ctacttaaag caatggacat taaagtcgtc
     8581 ttgaatgatg gaacattggt cggcaaactt gctccagaaa ttgataagag cttggcgacg
     8641 ttgaatcgta gacgtgtagc atattagtgt tggattttca cattttgact gtatactata
     8701 actaacgagt gtttactgaa gagatggtgg taatcatatg ttgaagcata aatttaaagc
     8761 aaagaaacct gcactgatag gatatattct actgcttgct attggattat ttttaacggc
     8821 agtaagatgg tatagcggca taaatcatgg ttttgtattc atcaacaatc agattaattc
     8881 tcacatttca aacttcgcat taagctttct tttctatttg gtgataggct atctttggtt
     8941 gcttatggga acgaatttaa gggttcttac tgcgatggga gctggtctga ttgttgcaaa
     9001 tattgtatgt gaaacgatga tgacttttct gaatacacca gatctaatcg atgcaatata
     9061 tggaatttcc ggcgtgctgg ttgtatacgc gtatttagcg attaccaaga aaatagggct
     9121 ggaagaaaca accgaatagg atgatagatt ataaacttta ggattagaca ttgttgtctg
     9181 gtcctttttt agttatttag atgggcggtg gtcaattgaa cgcatttatt ttagacggaa
     9241 caattaattc acggactgat ttaggtttgc gaattacgga agtgccagtt attccagtga
     9301 cggaacgtgt cattgatagt attgagattg atggacgtga aggagatttg accattctga
     9361 aaggctggaa agatgtgact ttcggtttta aggtagtgat ttggaaatct gatgtttgga
     9421 ctagctggca gataattttg ccacaaatcc taaacgctaa aactatcgca ttttcaaatg
     9481 aaccaaacgt gttttaccca atcaagtatg ctaaggcgag cggactaact cagattttat
     9541 cttccatgtg ggagtttgaa atggagttaa cctgttcgcc ttttcgttat aagaccaatg
     9601 tagcgactat caatcgaacc acgtcaggaa cggtcaataa tcccggcaac atttactcat
     9661 tgccgaagat taaggtctac ggcaatggaa ctaggacact cacgataaat ggcaagccta
     9721 tcgtgttaaa tctgacgaat ggcaacctga cactagattc ggatttgaaa gaatgctttt
     9781 acggggatgt ggcagccaat caatacatga caggtgactt tcctgagttt cgagtcggca
     9841 gtaatactgt gactcttggt acaggtataa ccaaagtaga aatagaaccg aggtggcgat
     9901 acttatgatt acgctttaca acaaaacaga atcaaatttc aatcataacg gtctcgctgt
     9961 ccttgatggc aacattctga accccgtggt gagtgaggaa ttgaacggac tcttttcctt
    10021 tgaattcgat tatccaattc atgctccaca cagtgaggaa ttgttgccag aaatgattgt
    10081 gaaagcacca gtaccagaac tgtccgacca gcttttcagg attgttgaac gagatgatgc
    10141 acttggtggt tttttgcaca tagtagctca tcatattttc tatgacctag ccacaaactt
    10201 tattgaagat acttatatcg tcaacaagaa cgggtcggga gctttgacac aactgcttgg
    10261 tgcaactcaa ttcatgcacc gttttaccgg tacatcaaac atcagtgcgg tcaataatgt
    10321 ccgcttggtg cgactaaatc ctgtagaggt gttgcttgat agtgacctag agaatggttt
    10381 tcaaagcagt tatggtggtg aaattgtacg cgataacttc acgatttcaa tgttaacgac
    10441 tcgtggcagt aacaacggcg tacaaatccg agacaagaag aatctgacag gatataaagc
    10501 tgatgttgat tactctggaa ttgttactcg cattatgcca caagggtatg atggactatt
    10561 tcttcccgaa aaatacgtgg acagtccaaa catcaaccaa tacgtccagc ctaaaatcaa
    10621 agtcatcaag tacgacaacg tcaaagttgg tgatgaagag gatgagttta ctcaagccga
    10681 ggcctatgat gaacttcgca gattggctgg gttagaatac tcagctaaca aggtcgattt
    10741 gccaaaagcc acctatgatg tggagtttgc cccgcttgag cgaactgaag aatataaaga
    10801 ttttgcacag cttgaaacga ttaatttggg tgatactgtt tcagttattc atgccgaaga
    10861 tggattgaac atcacagcta gaatggtctc atacaaatac gatccatttc tgaaagcgta
    10921 tatctcagta acactcggaa acatcacgcc aaaattcacg gatgtggcga aggacatcaa
    10981 ccgggtggac aacaaggcgg agcaagccaa agacgatgcc aattacgcat tgagttcggc
    11041 aaatggtaaa aacaccaact tctatggccc gacaactcca aataatccga aactcggtga
    11101 cgtttggtac aaagaaaatg gtgacaagtt tgaaatgtgg gtgtatgaaa tccgtgatgg
    11161 cgtcactcag tggtatccac ttatgacaga tatgacggca gaagaagtaa agcaagccgt
    11221 ggaacaagca caaaaggatg ccaatactgc cactgaaaag gccaatcaag catttaacga
    11281 tgctgtgaat gctttgaatg cagctgaaag tgtaggtgca agtgttgaaa cgcttactac
    11341 atccgttgaa cagaatacta gcaagatttc attagtggcg agtggtatgt caactcttga
    11401 aggacgagta atcacagctg aatcaactct gacagtgcaa gctgggcaga ttgctagtaa
    11461 ggcaagtcaa tcgacggttg ataatctgac agggcgtatt acaactgcag aaagcaccat
    11521 cacccaaaat gctgatagat ttgagctatc tctttctaaa acaaataaag cggtcaatgt
    11581 tttagccggt atgagcattg cagatttgca gtgggcagtt ggtggtctga atacaaatga
    11641 tggaaaagaa aatacctatg gtggatacgt tcgctcaggt tttacaagag ttagaaatgg
    11701 cgaaaagtat ctacttcaaa catttgtggg tgggaaactt tactctactt acgcaacggc
    11761 ttatctgctt tattacaaag aggataagtc ctttttatcg tacacccaat ttggaaacac
    11821 caccacaccg cttactgttc caagcagtgc cgcttttatt cgcattc
//
LOCUS       DAYYAG010000086        11837 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000086 DAYYAG010000000
VERSION     DAYYAG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.086"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACHARQ_002027"
     CDS             <1..177
                     /locus_tag="ACHARQ_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8410428.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACHARQ_002028"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACHARQ_002028"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8410429.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACHARQ_002029"
     CDS             1199..1771
                     /locus_tag="ACHARQ_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8410430.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACHARQ_002030"
     CDS             complement(2023..2280)
                     /locus_tag="ACHARQ_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410431.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACHARQ_002031"
     CDS             complement(2406..3221)
                     /locus_tag="ACHARQ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410432.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACHARQ_002032"
     CDS             3738..5063
                     /locus_tag="ACHARQ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8410433.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACHARQ_002033"
     CDS             5056..5406
                     /locus_tag="ACHARQ_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410434.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="ACHARQ_002034"
     CDS             5388..5573
                     /locus_tag="ACHARQ_002034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410435.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHARQ_002035"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHARQ_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8410436.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACHARQ_002036"
     CDS             complement(6724..7677)
                     /locus_tag="ACHARQ_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8410437.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="ACHARQ_002037"
     CDS             8289..9782
                     /locus_tag="ACHARQ_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8410438.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="ACHARQ_002038"
     CDS             9917..10213
                     /locus_tag="ACHARQ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8410439.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="ACHARQ_002039"
     CDS             10314..11165
                     /locus_tag="ACHARQ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410440.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="ACHARQ_002040"
     CDS             11186..11398
                     /locus_tag="ACHARQ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410441.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4075 a         1820 c         2197 g         3745 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaacggtt
    11761 ctgttgcaaa gttttaaatc tactatcaaa taaggtagaa taatagaaaa agatagcagg
    11821 aggaatgacg atgaatc
//
LOCUS       DAYYAG010000087        11488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000087 DAYYAG010000000
VERSION     DAYYAG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.087"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..716)
                     /locus_tag="ACHARQ_002041"
     CDS             complement(111..716)
                     /locus_tag="ACHARQ_002041"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8410442.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            912..1835
                     /gene="coaA"
                     /locus_tag="ACHARQ_002042"
     CDS             912..1835
                     /gene="coaA"
                     /locus_tag="ACHARQ_002042"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8410443.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            1894..3459
                     /gene="guaA"
                     /locus_tag="ACHARQ_002043"
     CDS             1894..3459
                     /gene="guaA"
                     /locus_tag="ACHARQ_002043"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8410444.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(3950..5581)
                     /locus_tag="ACHARQ_002044"
     CDS             complement(3950..5581)
                     /locus_tag="ACHARQ_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8410445.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(5595..7268)
                     /locus_tag="ACHARQ_002045"
     CDS             complement(5595..7268)
                     /locus_tag="ACHARQ_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8410446.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(7281..7598)
                     /locus_tag="ACHARQ_002046"
     CDS             complement(7281..7598)
                     /locus_tag="ACHARQ_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8410447.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(7623..9023)
                     /gene="lacG"
                     /locus_tag="ACHARQ_002047"
     CDS             complement(7623..9023)
                     /gene="lacG"
                     /locus_tag="ACHARQ_002047"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8410448.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9260..10045
                     /locus_tag="ACHARQ_002048"
     CDS             9260..10045
                     /locus_tag="ACHARQ_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8410449.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10048..10740
                     /locus_tag="ACHARQ_002049"
     CDS             10048..10740
                     /locus_tag="ACHARQ_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8410450.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11014..11385)
                     /locus_tag="ACHARQ_002050"
     CDS             complement(11014..11385)
                     /locus_tag="ACHARQ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8410451.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3478 a         2469 c         2101 g         3440 t
ORIGIN      
        1 aaaaaatagt tctttgtttt taaaaacttc tcaacaaaaa aagaactgat acgtacccgt
       61 acatgcacgc ccagttccac tctttcgtcg ctccactatt tttcagctag ttattctttc
      121 acacttttga tgatataata tcctttatct tttgtgacga tttctgcatt accaaatgtt
      181 tcttccattt tcttttgcgc actcggcgct ccttgtttct tctggatcac aattgtcagt
      241 gtaccgccct tcttcaaccg aggatatgcg cctgtcaaaa tttcatgtac aacttttttt
      301 ccagcacgga tcggcggatt gcttacgata gctgcatagg tttcttcatg aactgcttca
      361 tagatattcg attgatggat atcaacatgt tcgatttggt tacgctttgc gtttccttgc
      421 gctagactta ccgctcgttc attaatgtcg accatttcaa caaaccgctg tgtcgcaaat
      481 gccaaagcta acccaatcgg tccatacccg catcccacat ctaagatctt tccttcatca
      541 ggtaactctt cccaatcgaa agcatcaatc aggacacgtg aaccataatc cactgtttca
      601 cgtgaaaata caccagaatc tgttacaaat tgaaaggttc tgccttttaa ctcaaaagcc
      661 cattgttcaa aatcgtgcgc cgtatctggg cgttcagaat agtaatgatt tgtcatgtct
      721 tccatccttc tcatttcttt catgacaaca gtctatcctt ttatgtatcc gtgcgcaagt
      781 caagaataag actccgtttc tttctattat aaagattata caaaaagatc ggcaatctct
      841 cttaagtggt ggggtttgcg tttattttgt gttaaaatag ttccattgat gggagaacgc
      901 aggaggctaa tatgaatgat ccaatgaact actaccgtgt ggcaagagaa gaatggcgag
      961 aattttatcg taatgggcaa gcacctttga cacaagatga actagataat atcaaaagtt
     1021 tgaatgaccg catttccatg caggatgttc gagatatcta tgtacctctt gctcatctga
     1081 tttatcttta tatgaaagaa tttgaatctt tgttcttgag taaaggttta tttcttcata
     1141 aatacgtaga agtaccgcct tttattatcg ggatcgccgg aagtgtagcc gttggaaaaa
     1201 gtacaacagc cagactgctg cagacgattt tgtctcgtac cttcaaacga agaaacgttc
     1261 agctgatcac gactgacgga tttctttatc caaatcgtat attaaaagag cgtggaatca
     1321 tggaccgtaa aggatttcct gaaagttatg atatggagat gctgatcgat ttcttgaacc
     1381 gtgtaaaatc tggtcaggaa gagataaaag tacctgttta ctcacatgat gtgtatgata
     1441 tcattccaga caagtacgaa ttgatccagc agcctgatat cttgatcgtt gaggggatca
     1501 atacgcttca attacctgcg aatcagcaaa tctacgtcag cgactttttt gatttttctg
     1561 tctatgtcga tgccgatccc gtgctgatcg aaaaatggta tttggatcgt tttggtgcgt
     1621 tactggatac ggctttccaa aatccagaga attattatta tcgttttgct atcggagatc
     1681 gtgaagaagc ctttaaaatg gcaaaagatg tatggaaaaa tgtgaatttg aaaaatttac
     1741 aggaatatat cttgcctaca cgaggaagag ccgatataat tctccataaa actgaaaatc
     1801 atatcatcga ttcgattttc atgcgaaaat attagcattt taaaatagtt tgactatttt
     1861 gagataaaat ttaaagggat aggggtaata aatttgacaa acgttgccga tatgacgaac
     1921 gttgaaaaga ttatcgtatt agactacggt agtcagtata accaattgat cacaagacgt
     1981 attcgtgatc ttggagtctt ttccgaatta ttgagccacc gcattactgc agaagaagta
     2041 aaagcgatga atccaaaggg aatcattctt tctggcggac caaacagtgt atatgatgag
     2101 aatgcatttg ggatcgatcc tgagatcttc aatttaggta ttcctgtatt agggatttgc
     2161 tatggtatgc aattgatcac gtacaaactt ggcggttccg tagagcctgc taaaaaccgc
     2221 gaatatggga aagccttgtt agaagtaaca agcgatcaag ctgtcttgtt taaagaaaca
     2281 ccaaaacaac aaacagtatg gatgagccat ggtgacttgg taactaaagt tcctgaaggc
     2341 tttgaaacag tcggaacaag caaagattgt ccgattgctt ctatccaaga cacaacacgt
     2401 aatttttatg gtatccaatt ccatgcagaa gtacgtcatt ccgaatacgg aatcgaattg
     2461 ctgcgccact ttgcctttga tgtttgtcac tgtgaaggta actggacaat ggataacttt
     2521 atcgatatgc aagtagaaaa aatccgtgaa caagttggtg acaaaaaagt cttgcttggt
     2581 ctttcaggtg gcgttgattc tagtgtcgtt ggggttcttt tacaaaaagc gatcggtgac
     2641 caattgacat ctatctttgt tgaccacggt ctgctacgca aaggcgaagc ggaacaagta
     2701 atggaaagtc taggcggaaa attcggcttg aacatcatca aagtcaatgc aagagatcgc
     2761 ttcttagaca agttgaaagg tgtatctgac ccagaacaaa aacgtaaaat cattgggaat
     2821 gaattcgtat atgtttttga tgacgaagca acaaaacttg ctggtgaaga aggcgtctct
     2881 ttcctagcac aaggaacgtt gtatactgac gttatcgaat ctggtacaga aacagcacag
     2941 acgatcaaat ctcaccataa tgtaggtgga ctgccagaag atatgcaatt tgaattgatc
     3001 gaaccattaa acacattatt caaagacgaa gtccgtgaat tagggactca gttaggcatg
     3061 cctgattcaa tcgtttggcg tcagccattc cctggtcctg gtcttggtat tcgtgttctt
     3121 ggcgaaatca ctgaagacaa gctagaaatc gtacgcgaat ctgatgctat cttgcgtgaa
     3181 gaaatcgcag ctgctggact tgaccgtgat atctggcaat atttcactgt tcttccaggg
     3241 atccgctctg tcggtgttat gggtgacgga cgtacttacg actacacagt cggtatccgt
     3301 gctgtaactt caatcgacgg aatgaccgct gactttgccc gtatcccatg ggaagtcttg
     3361 caaaaaatct ctgtacgtat cgtaaacgaa gtagcccacg tcaaccgtat cgtgtatgac
     3421 atcacttcta aaccaccagc aacagtggag tgggaataaa gagccaacat aatatggagg
     3481 gggaatccct ataaatcaac attcttcaat agctaaaatg cttgattttc attaaaaata
     3541 agatggttcc ctaccaaaag agactataat ataagttaat ttgtttcatt cttatcataa
     3601 aaagaagttc tcgaaaaacg aactgttttc cgagaacttt tttctatcca tctaagtgct
     3661 caaatcatat tcacgacgga attctaaggt taccctttct taaacaaact ttatgttatc
     3721 tgcacaactt tttgcaattt tttcattgct tcttacgaaa tcgttgaact ctctttagca
     3781 taatacaaag tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat gactggaaat
     3841 cgtttctggt tatttttggc aaaggcgagg tgcaggttcg attcctgcct tctacgctaa
     3901 cgatgtggct gaagggaacc tccgatcttg aatcgaaacg atccgcagtc tattgtttca
     3961 ccaatgaaac aaaatcaacg ttcgtccaag catttcctga agccttgcta taaaccccaa
     4021 tttcacaaac acctgttgta attggaatgt ctttgattgt aatttttgtc cattcacttg
     4081 ttttaacaac cgcagtattt agttccgctc caccataatt tttgatatat aactgattag
     4141 tatcaaactc tcccccagac attacatagg cactcaatgt gtaagttcct ttgcttatgc
     4201 cactaacatt ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa gttagtttgt
     4261 agtccccaaa aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt ccttgttgaa
     4321 accaggtttg ccaattggaa ggactggtcg ttggtccatc gctttcgaaa cttgcatttt
     4381 taactagatt tatttgcgcc cctgcttgtt taaacgcacg caagcttggc aaggcgtttc
     4441 cagcaaaatc aaacatcgct tggttgtccc attcgtttcc catagtggat tgaacactga
     4501 gataattcat acctgcttgt gttgcccaac tcacatttcc gttgtaccaa agaggttccc
     4561 aatagaagaa gcccgccccg cgattattcg gtactgcctg aatccgatcg gcaagatcca
     4621 gcaaatactc tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct gcttcggctt
     4681 caccaaaagc atttggcata gtatcggcat tcgctgttgt atgcccataa gctgtttcaa
     4741 ctacgatcac ttctttacca taccgttggc tgatcgcatt catattggtt tgaagttcat
     4801 tcattgaacc gtgccaatac ggataatacg acactccaat taaatcaaat gacacacctc
     4861 gactcgtgat ttcatcaaac caccaagtaa atgctcctgt atcgccacca tcagctaagt
     4921 ggagcattac tggagttgtt ttaggttgag tgtctcgaac agcttggata ccagacttca
     4981 aaaaagcagc caatcgatca aattctcccc cgccttctcc ccaagacttg ccataaggcc
     5041 agagcattcc gctattcagt tcattgccga tctgaaccat atcaggataa acgtccagct
     5101 gcttcatttc cgacagaacg ttctttgtat agtcgtacaa tgccgtattc atttcttcaa
     5161 aggtcagacc ttgccatgct ttaggaagat tctgtttccc aggatcaacc caaaaatcac
     5221 tgtagtgaaa atccagcagc accttaagtc ccagattttt tgctcgtttt gcaaggtcaa
     5281 tcgtcgtttc cagatcattt gtccctgctc cataactttc tccattacta ttataaggat
     5341 cctgccacaa acgcagtcga acatagttga caccattatt ttttaaaatc tctaaggcat
     5401 cttttgaaat atcgtcttca taatattttg caccagattt ttccatatct gacaggatcg
     5461 aaatatctgc tccgcgaata aattcgctgg cagatgcttc aacagttttt gaaacagccc
     5521 ccaaagcccc taacacagct gcaaataaaa cagctttacg caaccaaatt cccattttca
     5581 ttatggctcc tcctttattg aaattggttc agaataaact gaacagcttt ttcaggatct
     5641 ctcgttaatc cgatatattc tgctcctttt gtcgcaacca gcttgattcc taagcgatcg
     5701 gtgtcttctt tgatatcatc atagtaagaa ttgacttgcg gtgctaagac caccatatca
     5761 aaatcctgca gcatttcgta atgagaacca taagcacccg ccattgcact gatcggtgct
     5821 ttcgattcag atgcaccttt ttttaaagca ttagctaaca tcccacttgt ccctgcgcca
     5881 gcacaaagta ccaacacatt gatttcttta tttgcatcgc tgataacttg actcgctgcc
     5941 acaggtactt cctcacgtac atcggcagaa gcaccttcct cggttgcgat cacctctgct
     6001 ttttgtgctt ctttctctac taagacagca tcgtatgctt tgcaaaatgg tagatagatc
     6061 aggaagtcaa cgacgattag aaccgctgct agaacaaaag ctaagatacc catgcccgtt
     6121 cctagaacca aaccgatcgg tgctggagtt gcccaaggca aaacgtacat aaagctgttc
     6181 atgcccaata catcgacaaa aaacttgaac aggcaaacgt ttacaacagg tgttaacaaa
     6241 aatggaataa aaaaataagg gttcaaaatc atgggtgccg caaatagcaa tggttcgttg
     6301 acagcgaaac ttactggaat aaaggaagct ttcccaactg ccttcaactg cttagactta
     6361 gcaaagagta ggaagataaa aggaacaacc aatgttgccc cagttcctcc catagttcct
     6421 acaaaattcc ctaaacccac cgtcagtact ttagaagctt gttcaccgga attgaacaat
     6481 tgcagattgg tttccacatt ggcatagata attgccgcaa ttgccggctc cacgatcgac
     6541 ggtccatgaa caccgacaaa ccaaaacagc gccatcgctc cccagataat agcgattcct
     6601 aagtacccgt cagctgctgt aaatagcggc tgcagcaccg taatgattgc ttctgcaaat
     6661 gagatttgga agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa tactgaaaaa
     6721 gagaaaggaa aaatatctcg gaaggtttgc gaaatcgttc caggaacttc tttaggcatt
     6781 ttaatggtaa tatctttcag cacacaaaac ttatataaat taactgttaa aaatgcagcg
     6841 acaaaggaag ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc accttcaatc
     6901 ggagagacgc ttaacaacag gaaacttacc acagaggcca gcatcacaga ggtggtgttg
     6961 attactttgt ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg agcagtggtt
     7021 gctgaaacga gtaaccctac aacacccatc gaataattat agactttcca tagccatgtg
     7081 gagacagatt caggccattg gtaaccaaaa atctcaggaa ccgcagcaat caaaatgaaa
     7141 atgcttgaaa ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac ggctcctaaa
     7201 taactattac gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg tttaatgatt
     7261 ccatccatag cgttcttctc ctatccttga ttctgtctgt acagtacaat aaagtcttga
     7321 atcagatctc tcagcaataa agtggtcatt aaatgatcct gcccatggat aaaaatgaat
     7381 cccatctcca tctctttccc agatgcttct tccgctaaaa tttttgtttg cgaattatgt
     7441 gcaagggtta aattttcgtc ggcttctttc aacgccgaat caacatgttc aaagtttcct
     7501 tgtcttactt ctttcaataa tttcaataat gtactgcgag cgtcgcctga atatgcaacg
     7561 atctcaaaac caagcatttg taattcctct ttattcatct tttcttcctc cattatcact
     7621 tattaaataa tcgttttggt ttcactgacc aacttgtacc agtaagctga ttcttttggg
     7681 taacgcttct gggtttcaaa atcaacataa aacaagccat accttttgtt atacccattt
     7741 gtccatgaga aaagatccat aagcgaccac aaaaaatacc ccttcacatt taccccggct
     7801 gttatcgcct tgcttaagga ttccaaatag actctcaaat agtcgattct cggctgatcc
     7861 ataataatgc catcttcaaa ctgatcttta taccccatcc cgttctcagt gatatagatt
     7921 ttgttgtaat gtgggtaatc gcttttaatt cgcagcaaca agtcatatag accttctgga
     7981 taaatcagcc agtcccaatc agttcgagga attccttctt tataaatacg ttctccgatt
     8041 cccttcactt tataaacaga ggtccctttt tcacccgtac cgttatgatg gattgcattt
     8101 tctccatcgt aagctttgac aaaatggcat tggtagtgat tgatccctaa gtagtcatta
     8161 cgcgtggatg cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata gctggcctga
     8221 tttgcttcac atatctcatc aagagcagtc aaggtttcag ttgaataata gcctaaatag
     8281 gtagcatcta ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc atgcttatct
     8341 tccggcgcgt ctgttgccgc atatttcgtc tctaatgagt gaactacacc aatttcaccc
     8401 ggaagttcgt tttctttgaa atagttcact acacgggcat gagcaaccat catattatga
     8461 agacaggcga cgatctttgt aaaatcatat ttgattcctg gtgggaaaac acctaacaaa
     8521 tattgattgg ttgcaactgg atagatttca ttaaaggtac tccacacctt gacttctttg
     8581 aattcatgaa aacaaaaaat ggcataggaa acaaatgctt ctattgtttc tcgattcaaa
     8641 aaatctccat ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt cacaaacggc
     8701 gttacgtttc ttttcttaca ttcttcgaac acacgatgat aaaaagcgat tccttctgga
     8761 tttggtttcc cagctccatc aggaaaaatg cggctccaag caatggataa tcttagacca
     8821 tttacaccaa aacgttcaca caattcaatg tcctttgcat attgatgata aaaatcactt
     8881 gccggatcgg gactgaatcg cccttgttcc tctaaaaagt catcccaagc aacagcgcct
     8941 ttgccacctt ccttagtggc tccttcaacc tgataggctg ctgtggctcc gccaaaaata
     9001 aaatcttctg gtaatttaag catgtacatc tctcctcaaa tctataatca gttttcgctc
     9061 tttctaactg attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa caaacacaaa
     9121 taatcataaa aaccagaaaa cagcaataaa atctattgta acaaacataa aaaccaccct
     9181 aaatgtttac ttttgttcga attagattga ctgttgtccg tttatatgat agattatcct
     9241 taaattagag gtgaagacaa tgttgaaaaa agaaagattg cttactatcg tagaaatggt
     9301 caataaaaaa ggaatcctta ccgttaatga aatcatcaat caattagatg tttctgatat
     9361 gaccgttcgc agagatttag atgaattaga aaaatcgggc aagctgctgc gtgtccatgg
     9421 aggggctcaa agtctttcct acactttaga tcaagaactt tctcacactg aaaaagccac
     9481 tctgcagatt caagaaaaaa aagaaattgc tgccacagca gcctccttga ttcacgaggg
     9541 agaaacgatt ttcttaggac caggtacaac aatcgaacta ttggcgacgc atttactgaa
     9601 caagaaaatt cggatcatta caaataatta tccagttttt gatattcttg ctcaagcaga
     9661 tggaccagaa attctactga ttggcggtga tttccggaaa aataccggcg catttgtcgg
     9721 accgatcacc aacgacaatt taagaagact aaagtttaca aaagcattta tcagttcaaa
     9781 tggcgttcac aacgaagcga tctctactta cagtaccgaa gaaggagaag cacagcagat
     9841 cgctctaaat aattcccgta agaaatatct actcgttgat aataaaaaat tcaatcgaga
     9901 tgatttttat gtgttttata atttgcatga tttcgatctg ctcattacgg atcgccagat
     9961 ctcgcgggag gttcaagagc actatgcaca atatgtcgat ctgcaaattg ctgattcaac
    10021 cgaaaatacc actataggag cgtaactatg aaagggatta tatttgattt caacggaaca
    10081 atgtttttgg actctcatct gcatgaagct gcttggctgc atatgataca tgatcataca
    10141 aaagatggtt tatcagacga ggatatttta aaaaatattc atggtcggac gaatacggag
    10201 attttgaaac atttcatttc agagcatttg accgcctctg agatcgccgt attgtcagaa
    10261 gaaaaagaaa gctattatcg tatgctttgc ttgcaaaatg aaaacgagct tcagttgaca
    10321 aaagggcttg aagcgactct tgaccagctg actgaccaac agattccatt aacgattgcc
    10381 acggctactg tcaaagagaa tgttgctttt tattttgata ttttcgattt agcccgctgg
    10441 tttgactggg acaagattgt ctatgacgac ggctctttcc ctgggaaacc gcaaccggat
    10501 atttttctta aagcggcaga caaactgagc ttagctccgg ctgattgtct tgttatcgaa
    10561 gatgcttact caggattact tgctgccaaa cgtgcgggga tcggaacaat tattgcaatt
    10621 gatccattcg gtaaaaacaa aacagtattt gaacaggcac agctaaataa agatggaacc
    10681 atcaaagatt ttactggttt ttggacagag cacctgtctg ccacatccgt taattcttaa
    10741 acgattcaac taactaccaa agaaaagcaa ctgaaatgca cacctccttt tataaaaaac
    10801 tgccagtgaa acgacatgaa agatacactg ctatatcctt agtgaaatat agcagtgtat
    10861 ctttgacttt ttcattcttt taaccatatg acgcgttcat ctctatctat aattaacatt
    10921 aaaaatagtg ttttcacatt atttctcaaa ccaatcttaa aaatcatcag gagtactttt
    10981 ttgatatact tttattaatg ctgggcgtaa tggttaaaag atttccacta gttcctctcc
    11041 accaagctga gttgaaaaat gcttatctgt aaacaacata gacaaagagg aaataaacgt
    11101 ttccgtatct ttctctgtcc caaggatatt aattaatttt cctactaggt aagtatcatt
    11161 aaacttaaga tctgccttca catcaaaagt tttgataaaa gcttttatca tgcgtacaat
    11221 tttgtcttta tcgccataat tgatttcacc actcatatca atatagtcac taatccctga
    11281 agaaataatg gcaactgctt cttctttcgc cttatcagat aattcattta atgactggga
    11341 ttgattgatg acttcttgag ttttatctgt tacgtaatct ctcattatcc tatcggcata
    11401 ctccgcaaag taatcttgaa aaaattcttc tacagggtaa ccaacttcgt tttcccaacc
    11461 aggagaaatt tcattatcag tacttatt
//
LOCUS       DAYYAG010000088        11267 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000088 DAYYAG010000000
VERSION     DAYYAG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.089"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..1822
                     /locus_tag="ACHARQ_002051"
     CDS             161..1822
                     /locus_tag="ACHARQ_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8410452.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1892..2254)
                     /locus_tag="ACHARQ_002052"
     CDS             complement(1892..2254)
                     /locus_tag="ACHARQ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8410453.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2322..3263
                     /gene="opp3b"
                     /locus_tag="ACHARQ_002053"
     CDS             2322..3263
                     /gene="opp3b"
                     /locus_tag="ACHARQ_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8410454.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3264..4307
                     /locus_tag="ACHARQ_002054"
     CDS             3264..4307
                     /locus_tag="ACHARQ_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8410455.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4313..5383
                     /locus_tag="ACHARQ_002055"
     CDS             4313..5383
                     /locus_tag="ACHARQ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410456.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5373..6326
                     /locus_tag="ACHARQ_002056"
     CDS             5373..6326
                     /locus_tag="ACHARQ_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410457.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6338..7348
                     /locus_tag="ACHARQ_002057"
     CDS             6338..7348
                     /locus_tag="ACHARQ_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8410458.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7633..8454)
                     /locus_tag="ACHARQ_002058"
     CDS             complement(7633..8454)
                     /locus_tag="ACHARQ_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8410459.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8551..8829)
                     /locus_tag="ACHARQ_002059"
     CDS             complement(8551..8829)
                     /locus_tag="ACHARQ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410460.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9097..11106
                     /gene="metG"
                     /locus_tag="ACHARQ_002060"
     CDS             9097..11106
                     /gene="metG"
                     /locus_tag="ACHARQ_002060"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8410461.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT         3754 a         2059 c         2288 g         3166 t
ORIGIN      
        1 ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt atgaaaaaga
       61 caaaaaaaag tgttgatttt ttaataatta ttcattaaac tcttaaacaa cttcagaaaa
      121 tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt
      181 tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac
      241 aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt
      301 gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc
      361 attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc
      421 aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg
      481 agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg
      541 gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga
      601 aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt
      661 tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct
      721 tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta
      781 tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg
      841 accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac
      901 agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt
      961 attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc
     1021 aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca
     1081 acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat
     1141 cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt
     1201 gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg
     1261 aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga
     1321 attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa
     1381 agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc
     1441 aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt
     1501 actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac
     1561 aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc
     1621 agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat
     1681 caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct
     1741 agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa
     1801 atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg
     1861 gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa
     1921 tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc
     1981 tggcagacga gagcgtggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat
     2041 ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga
     2101 taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg
     2161 aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg
     2221 ggatagattt tttttcattt gattgctcct ttatttttgg ttcattatct aataatgtag
     2281 cgaaacatct gtcagaagtc aaaagctagg gggaaacatt agtgaatagt tatatcaaat
     2341 atgtattaaa acgtgtcttt ttcatgatca tcacgctttg gttaattgca acaatcacct
     2401 ttttcttgat gcaattgctg ccgggcacgc catatacgaa tcaggaacgt ttgagtcctg
     2461 aaacaatcga gatgctaaat aaacaagtcg gtttggataa accagttatc gtacaatatg
     2521 ggatttatct ttctaatttg cttcaaggag attttggaat ttccttccaa tttaaaaacc
     2581 agccagtcgc acatttgtta gctggaagaa tcggaccatc cttgcagtta gggttacagg
     2641 caattatctt tggaacagtc ctagggacga ttttaggaac gatctctgcc atgaagcaaa
     2701 atacatgggc agatacatct tctacactcg tagcgatatt aggtcgttcc attccaaact
     2761 ttgtatttgc tgttttatta caatatatct ttgcaatcaa attacaagtt ttgccaatcg
     2821 caaaatggga tggctttgtg tacacaatct taccaacaat tgcgttggcc atgtcgccgc
     2881 ttgctgactc tgctcggttt atccgtacgg aaatggtgga agtactgcat agtgattatg
     2941 tggaattagc aaaagcaaaa ggattgagcc gttgggaaat cgcctttaaa catggtcttc
     3001 gaaatagttt gattccattg atgactttat taggaccgtt agctgtagca ttgatgaccg
     3061 gatcactggt ggtagaaaat atctttgcta ttcctggtat cggggaacaa ttcgttaagt
     3121 caatcacaac aaatgattat cctacgatca tggctgtaac gatcctttac tcatttatgc
     3181 tgatttttgt tattttgatc gtggatctat tgtacggtct agttgatcca agaattcgtg
     3241 tttcagaagg gagccgagga taaatggaaa tgataccaaa gcgttatgaa acagttgcag
     3301 atatccctgc ggatgaattt cttccattac aaaaaaatac cgaagaagaa cgtgaacaga
     3361 ttactgcacc ttctttgaat tttatccaag attcatggcg ccgtttaaag aaaaacaaag
     3421 ctgccgttat ttcaatggtt ttactattgg tcatcatttt gatctctgtg atcacaatct
     3481 ttgtttctcc tcatgatcca actgcccaaa acgtggctta tatcaatttg cctcctcgta
     3541 ttccgggaat caatatcgat ggattgaatg gaaaagcaat ggttggtggt gaacttgttg
     3601 ataaatatgc tcaagcaaac gtcccttcaa acgtaaattt ctttttagga acagacggtt
     3661 tgggacgtga tgtattaagc cgcttattta tgggaatgag aatctcgttg ctgatcgcat
     3721 ttattgctgc attactagat gtaacgattg gtgtagctta cgggttggtt tctggtttgt
     3781 taggcggacg tgtcgataac gtgatgcaac gttttttgga aatcttatca ggaattccaa
     3841 acctcgttgt catgattttg atgttggtcg tttttgaacc aggaattttt tcaatcgtgg
     3901 ctgctatggc catcacaaac tggataccaa tggcacgtat cgttcgtgca caaacaatga
     3961 aattaaaaga tcaagaatat gttttagctg gattgacatt aggtgaatca agaatgaaaa
     4021 ttgcattcaa acatattttg ccgaatatct ctagtgtcat tattattcaa atgatgttta
     4081 gtattccatc tgctattttc ttcgaagcct ttttaagctt tatcggttta ggtcttacgc
     4141 ctccgtcagc ttcattagga acgatgttga gcgaagggta caagacattc ctgtatctgc
     4201 cttatctatt atggattccg gcagtaactt tatcggttat catgattggc tttaacttac
     4261 ttgctgatgg attgcgtgat gcctttgatc ctaaaatgaa agagtgaaga cgatgacgaa
     4321 aattttagaa gtgaaagact tagaaatctc ttttgatacg tatgctggaa aggtacgagc
     4381 gattcgcggt gtcaattttc atttgaataa aggagaaaca ttggcgatcg ttggggaatc
     4441 tggaagtgga aaatcagtga cgaccagaag catcatgcgc ttgctgtcaa gcaacgccaa
     4501 tatagatgct ggtcaaatcc tctttaaagg taacgatatc gttggaaaat cagaaagaga
     4561 gatgcaaaaa atccgtggta aagaaatagc gatgattttc caagatccaa tgacctcttt
     4621 agatccgacg atgcccattg ggaagcaagt cgctgagtcc cttagaaagc ataataaagt
     4681 ttcaaaaaaa gagggacaaa aagctgcatt agaattgctt aaattagttg gaatacccga
     4741 agctgagaaa cgtatcaaca gttatccgca tcagttctca ggaggtcagc gtcaacggat
     4801 cgtgatcgcg attgcactgg tatgttatcc ggaaatcctg attgctgatg aacctacaac
     4861 agcgcttgat gtgactattc aagcacagat tttggagctg ctgaaagaaa ttcagacaaa
     4921 aatcgataca tcaattatct ttatcactca tgaccttggt gtagtagcga atgtcgctga
     4981 tcgtgtcgct gtcatgtatg gaggaagaat cgttgaagtt ggaacatcgg aagaaatttt
     5041 ctacaacccg caacatccct atacttgggg actgcttggt tcaatgccga cattagatag
     5101 tgcgaacgat cgattgtatg ctattcctgg ttctccaccg gatttattga acccgccaaa
     5161 aggagacgca ttctatccaa gaaatgaatt cgcgatgaaa atcgatgcag aacaagaacc
     5221 accatttttt gaactatcaa aaacacacaa ggcagcaact tggctacttg ctccgcaggc
     5281 acccaaagtg acaccgccag aagaaatcca acgtcgctgg gctatcttta aagagaaaca
     5341 aaaacatact tacggtcaag gaggaattgc tgatgccaaa taaactactt gaagttaaag
     5401 gattgaaaca gtatttcaat gtaggaaaaa agaatgaagt acatgctgta aatgacatca
     5461 gctttcacat ttatgaagga gaaacattcg gtttagttgg agaatctgga agcggaaaat
     5521 ccactactgg ccgtacgatc attcgcttga atgaacctac tgatggtgag atattattcg
     5581 acggtcaaga tataacgaag atcaaagata aaaaaggttt gacgaaattc cggcatgatg
     5641 ttcagatgat ctttcaagat ccatacgctt cgttgaatcc tcgtatgaaa gtcagagata
     5701 tcattgccga aggaattgat gttaatggac tagctaaatc atctgaagag cgtgcaaaaa
     5761 aagtcgatga tctattgaga actgttggat tgaatccaag ccacggtact cgttatcctc
     5821 atgaattttc aggtgggcaa cgccagcgta ttggtattgc acgagcattg gcagtaaaac
     5881 cgcgttttat catttgtgat gaaccaattt ctgcactaga tgtgtcgatc caagcccaag
     5941 tggtcaattt gttgcaagat ctccaaaaag aacatcaatt gacctatcta tttattgcac
     6001 atgatttgtc gatggttaaa catattagtg accgaatcgg tgtaatgcat aacggcttgt
     6061 tattagagat gggaacaagt gatgaaatct ataatcacgg tgttcatcct tatacagaaa
     6121 gtttgttatc tgctattcct ctaccagatc cagaccatga gagaaaacgc agaagaatca
     6181 aatatcaacc tgaaccagat gacggacaag tacgtcaact tcgtgaaatc gcaccagaac
     6241 attttgtcta tgcaactgag caggaagtcc catattacgc aaaaaaacta aaacgacaaa
     6301 aggaatctct tctggttgct aactaggagg gacatagatg ctaaaagctt ttaaattccg
     6361 tatgtatccg acagaagaac aaaaacagca attgattcgt acttttggat gtgctcgatt
     6421 tacctataac catttgctga aaaagcggca gaaaagttgg caacaaacag gagtagccaa
     6481 tttttcgctc acacccgcta cattaaaaaa agaataccca ttcttaaaag aagtagacag
     6541 tcttgcttta gcaaatgcac agcttaattt ggatcgggct tttcgcaatt atttcaaggg
     6601 acgtgccagc ttcccaaagc ttaaaacgaa aaaaagtatg tggcaatctt atacgaccaa
     6661 caaccaaacc cgtacgatct atctggaaaa tggctactta aagttaccaa aacaaaaaga
     6721 actaatcaag ataaatcagc atcggccagt tgaaggatcg attcgctccg ctacgatttc
     6781 tgctcgatat aatgaagaat tttatgtcgc actactttgt gatgtttcgc cagtaaaaaa
     6841 agaatcttta gctaaatgga tcgggatcgc ctatcatccg aagacattga tccaaacatc
     6901 tcgacctcta gaagtaactt taccaaaatt tcaccagaca gaagaaaagc tgcaacatgc
     6961 acagcggaaa ttgaatgtga aagtccgctc cgctcaccat agaaagatca ggttagatca
     7021 ggcaagcaat tatcagaaac aaaagagaaa agtaatggat ctttatctga aacaaaagaa
     7081 ccagcgagaa gattatcttg aacaattatc tgggaaattg gtcaaacaat atgactactt
     7141 atttgtagaa tcttttccaa aagaagaggc acatgctgat ttttctattc acgactggca
     7201 taaattaata acaaaattgc gttataagtc ccaatggtat aataaaaaat ttcttttgat
     7261 caataccgac ggagcagaag aaagcaattc cgtaagaaag agccaagtag tagaaaaaat
     7321 gggcagacat tcggttatca agggataaaa aatgaatgaa actgatagga gcgttagggt
     7381 tagccttgtt aataaaaagt cttaatgact tttgtttcta ggaatttttc ttttgaagaa
     7441 aagctcacta attatgacaa accaataaaa caatagcaga aaaagttcgt acgagctttt
     7501 tctgctattg ttaatttttt attcagaaag tttacgaata catcaaaatt ctgaaacaaa
     7561 aaacgtaact cacaaagagt tgtttttgcg ttttttcttg aatgattagg tctgaatctt
     7621 ttgctcacaa ccttattcat ggtgccccgg cttgagctga tagatttttg aaggacggcc
     7681 gattcctaca ggacgttcac cgatctcatc gaaatattgg agcatagctt tcttaaaatt
     7741 ggaatgatcg atcgttttat aatcgatccc taagaattta gcaaatactt ttcgagcttc
     7801 cgtaatcgtg aaatctttac ctaaaacttg tagaacttgc ggctcgtgtt ccattttatt
     7861 gactacacgg ttgaatgctt tcaagatgat ttgactatgg tcaaaagcca aagtatcttt
     7921 gccagaagac tctccagtga ctaaatcaag cgagatagcc acttctccgc tagataaatg
     7981 gatcttgtta ctatgacgtt ctagtgtgaa ccagcgtact tccttggcat catctccggc
     8041 gattagcggt tcttctccaa taaaggctaa gtagctgact gtgacgaccc agcctcgcgg
     8101 atctcgatta ggcgtactga atgtatgcag ttgctcaata ttttcttttg aaataaccac
     8161 accagtttct tcctttgttt ctcttaagac gctttctcca gttgactcat ccttttggac
     8221 aaagccacca ggcaatgccc atgaatttcg ataaggatgc ccttttcttt ggatcaacaa
     8281 aactttcagc tgatcttctt ctttgttata gcacatcagt acgatatcga cagtcaaaga
     8341 gggcttgtca tattctggac gttcctgttt ttgataccag ttaagaaatt cttcttcact
     8401 tgctttatgt tcataatata atttttcttc ttctttagaa gcaaaacggt tcatctgacc
     8461 acctccattg acagtaaaag tactttttac cttattgtag cagactctga aaaaaaacca
     8521 aggatttact tgaaaaatct aggttatcat ttaaatatga aaaataaatg gaagtatgag
     8581 attgaataaa aagaaaagca gataaaacgg caatacttta taccgctctt cacgaatggc
     8641 aaagaagcca caaataacga tatatacgtc aatgataaaa cgcaaaatcc aagctgctgt
     8701 cataaaactg ttcaaatgcc catggaggtt aaaataacca tacatacgaa tataaaaata
     8761 gctgaatatt aaatgtgcta atgaaagaaa agcaatacta agtgtaaaaa aaagatgttt
     8821 cttctgcatg attgttccag tcccttacta agatagatag tctttattat accacgtgtg
     8881 aggggagaaa taaaaagccg ttttttagta gatatgaaac aaaacaaaaa cccgcattta
     8941 ttcaacctat tcatcctttt aaaggataga ttgaacgtag atagaagtcg tttatccagt
     9001 ctttttcatt ttgaaaaaaa gctaacagca tgcttttttt atggtatact agcaaggaaa
     9061 cgaattcgca tcatgcgata aaggaggaag agccccgtgg cagaaaagga aactttttat
     9121 atcactacgc caatctatta tccaagcggc aaacttcata tcggcaactc gtatactacg
     9181 attgcatgtg acgcaattgc tcgttataaa cggttgatgg gctttgatgt gttttatctc
     9241 actggagtgg atgaacatgg tcaaaaaatc gaaacaaaag cagaagaact aggtgtaaaa
     9301 ccgcaagaat atgtagataa aatggcggct gatgtcaaga aattatggaa aacgcttgat
     9361 atcagttatg ataaattcat tcgcacgaca gatgattacc ataaagcagc tgttcaaaaa
     9421 atatttgatc gtttattaga acagggagat atttacttag gtgaatatga aggctggtac
     9481 tctgtttctg acgaagaatt ctttacagaa acacaacttg cagaagtgta caaagatgac
     9541 gaaggtaacg tgatcggtgg taaagcgcca agtggacacg aagtagaatt ggtaaaagaa
     9601 gaatcatact tcttccgcat gagcaaatac gcagaccgtc tattggaata ctatgaagaa
     9661 catcccgaat tcatccagcc agaatctcgt aaaaacgaaa tgatcaacaa ctttatcaaa
     9721 ccaggattag aggatcttgc agtttcacga acaactttta cttggggagt accagtcaag
     9781 agtgatccga aacatgtggt gtatgtatgg atcgatgcat tatccaatta tattactgca
     9841 ctaggatatg gctccgaaga tgaaagtcta ttcgaaaaat actggcctgc agatgttcag
     9901 atggtcggta aagaaatcgt tcgctttcat acgatctatt ggccaatcat gctgatggcc
     9961 cttgatttgc ctctacctaa aaaagtattt gggcatggct ggctattgat gaaagacgga
    10021 aaaatgtcta aatcaaaagg aaatgtcgtt tatcctgaaa tgcttgtgga acgttatggt
    10081 ttagatgcac tccgttatta tttgcttcgt gctgttccgt ttgggtcaga tggtgtcttt
    10141 acgccagaag attttgtttc ccgtgtcaat tatgatctgg caaatgatct agggaactta
    10201 ttgaatcgaa cgattgctat gatcaacaaa tattgtgatg gactcgttcc tgactatgcc
    10261 tcacttgtca ctccatttga tagtgaactg tctacgacgg ctgctaacgt tgtcggacgt
    10321 taccatgatg caatggaaaa aatggaattc aatacagcat tggctgaaat ctgggtgctg
    10381 atttctcgtg caaacaaata cattgatgaa acagaaccat gggtattagc aaaagacgaa
    10441 gaaaagaaag ctgaattgaa tagtgtcatg atccatttag ctgaatcttt gcgtatcgca
    10501 gcgatattac ttcagccaat catgacagaa acgccagaga agatcttcaa tcaactagga
    10561 ttggactctg aaacaatgaa tcttgaagga ttacactttg gcgaatttcc ttcaggaaca
    10621 aaagtagttg caaaaggtac accaatcttc ccacgtttag atatggaaga agaagttgct
    10681 tttattcaag aaaaaatgtc tgaaggtact caaacaaacg aagatacagt caaatgggat
    10741 ccagaagaaa cagaacttgt ttcaacgaaa gaaaaacaaa tcaaatttga tgtatttgaa
    10801 aaagtagaac taaaagtagc agaagtcatc aactgtcaaa aagtagaagg ggcagataag
    10861 ttgctgcaat tccgactaga tgctggagac agccaagacc gtcaaatctt gtcaggaatc
    10921 gctgaatttt atcctgatcc aagcgaattg atcgggaaaa aagtcgtcat tgtagcaaat
    10981 ctgaaaccac gaaagatgcg tggacaaatc agtcaaggaa tgatcctttc tgcagaagca
    11041 ccagatggct ctttacaagt aatcgaagca ccaaaatcca tgccaaatgg ctcagaaatc
    11101 gcatagaaat tcaaaggggg tatgacacta gttgtgtcat accccctttt tccgaaattt
    11161 gagaagcaat tttcgtaaat tccttcttat tacttgaagc caatcatata atacagaaac
    11221 agagaactat ccaaaataaa tcctagaaaa agaagtacga gcttcgc
//
LOCUS       DAYYAG010000089        11230 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000089 DAYYAG010000000
VERSION     DAYYAG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.090"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1033
                     /locus_tag="ACHARQ_002061"
     CDS             <1..1033
                     /locus_tag="ACHARQ_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8410462.1"
                     /translation="ENAWDSLPEHLYKRHIDNVLILHGKTSWKAVKDVFPDLSITKIF
                     EYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKLIAYKTNLPIIILPTLAA
                     TCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHSPREMMIAGIGDTLAKWY
                     ESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAMDQQKINQAFIDVVETNI
                     LLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKVAYGILVQLMIENKLAEI
                     EHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEMIHFMKETITPALVVQKM
                     KELEQVTQG"
     gene            1367..2305
                     /gene="yidC"
                     /locus_tag="ACHARQ_002062"
     CDS             1367..2305
                     /gene="yidC"
                     /locus_tag="ACHARQ_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8410463.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(2486..2890)
                     /locus_tag="ACHARQ_002063"
     CDS             complement(2486..2890)
                     /locus_tag="ACHARQ_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8410464.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(2906..3715)
                     /locus_tag="ACHARQ_002064"
     CDS             complement(2906..3715)
                     /locus_tag="ACHARQ_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8410465.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(3702..4592)
                     /locus_tag="ACHARQ_002065"
     CDS             complement(3702..4592)
                     /locus_tag="ACHARQ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8410466.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(4605..5084)
                     /locus_tag="ACHARQ_002066"
     CDS             complement(4605..5084)
                     /locus_tag="ACHARQ_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8410467.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(5101..6867)
                     /locus_tag="ACHARQ_002067"
     CDS             complement(5101..6867)
                     /locus_tag="ACHARQ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM8410468.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(6913..7854)
                     /locus_tag="ACHARQ_002068"
     CDS             complement(6913..7854)
                     /locus_tag="ACHARQ_002068"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8410469.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(7851..8846)
                     /gene="lacD"
                     /locus_tag="ACHARQ_002069"
     CDS             complement(7851..8846)
                     /gene="lacD"
                     /locus_tag="ACHARQ_002069"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8410470.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(8839..10032)
                     /locus_tag="ACHARQ_002070"
     CDS             complement(8839..10032)
                     /locus_tag="ACHARQ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8410471.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(10050..10778)
                     /locus_tag="ACHARQ_002071"
     CDS             complement(10050..10778)
                     /locus_tag="ACHARQ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8410472.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         3214 a         2437 c         1984 g         3595 t
ORIGIN      
        1 agaaaacgca tgggattcac tccctgaaca cctttacaaa aggcatattg acaacgtttt
       61 gattctccat ggaaaaacat cttggaaagc agtaaaagat gtttttccag atttatcaat
      121 aacaaaaatt tttgaatatt atggtggaga atgtaccgat ccacttgctg acagttttgt
      181 ttctttatgc aaaaagaagg atttcaatgg tattattgca gtcggcggcg ggaaagtagc
      241 agatctagga aaattgattg cctataagac aaacttacca atcatcatct tacctacctt
      301 agctgcgacg tgcgcagctt atactccact tagtgtggtc tataatgaag aaggagtgat
      361 gcttcgctat gatatgttcc ctaaagcaaa tgatttagtg ttgatcgatc caaagatcat
      421 tttgcactcc cctcgcgaga tgatgattgc cggtattggt gacaccttag caaaatggta
      481 tgaatctcac gcaatcatct ctcagttaac tatcaagcct atcgaagtcc aagtagcgga
      541 atttgcagcg ttacgatgcc gcgaaatcct tttagagaaa agtgcagagg caatggaagc
      601 gatggaccag caaaaaatca atcaagcttt tatcgatgtc gtggaaacaa acattttatt
      661 ggctggtatg gttggtgggt ttggcgatga ctacggccga acagctggag ctcattccat
      721 tcacgatgcg atgaccgtac tccctgatag ccatcagcaa cttcatggaa acaaagttgc
      781 atacggtatc cttgtccagc tgatgatcga aaataaatta gcagaaatcg aacacctact
      841 gccgttttat catcacttga acttaccaac gagtttagct gatatgggtt tacatttaac
      901 agaaagtgac tatcaagccg tagctgaacg tgcatgttta cctgatgaaa tgatccactt
      961 catgaaagaa acgatcacac cagcactggt tgtccagaaa atgaaagagt tagaacaagt
     1021 gacacaggga tagttagccc cttataccat ggcagtcagc ttttttatgg atcggctcaa
     1081 tggtaaaaaa aaagaagtgg aaaaaattga cattccactt cttttttttg cgctaaaatt
     1141 atgatttcaa ggaaatatcc gttgctcatc ttattttaga gaacggaggt gctaagtatg
     1201 ctttatactg aagtattttt agctggattc ttctttttaa cattaagcgt cttttttgat
     1261 cctccaatcg gttatttcta tgaaatttct atgaagttga ttgaattatg agcgcttttt
     1321 tagtatagtt aagcttgtgt aaaaattata aaagaggaag tattttatga ataaatggaa
     1381 aaattggtta ttgggctctg ggcttgtcac gatcatgctt ttcttgtccg gttgtgtccg
     1441 catggattca aatggtaacc ctgacacttc aggaatcatt taccgtatat tggttcaccc
     1501 aatgggacaa gcgattacat atttagtgga aaactttaat tggtcctacg gctgggctgt
     1561 tatcttgatg acagtcatcg ttcgtatcat cattttgcca ttaggtatta gtcaatcgaa
     1621 aaaaacaatg atccaatctg aaaagatgca agcattgaaa ccacaagttg aagctacgca
     1681 acaaaaattg aaagctgcga caactcgaga agaacaaatg gctgcacaag ctgaaatgca
     1741 acaagtttat cgtgaaaatg gcttaagtat gactggtggt atcggttgtt taccgcttct
     1801 gatccaaatg ccgatctttt ctgcgctata tttcactgct cgctatacag aaggcatccg
     1861 tgaatcaacc ttttacggga tcgacctagg taaccctagc ttagttttag tagcgattgc
     1921 cggtattgct tatttacttc aaggctatat ctcaactatc ggtatccctg aagaacaaaa
     1981 gaaaacgatg cgtacgatgt tgatcgtatc acccgctatg atcgtcttca tgtcgatcag
     2041 tgccccagct ggtgtcactc tttactgggt agtcggtggg atctttagtt gtctacaaac
     2101 attcattacg aatgtcatca tgaaaccaag attaaaagcg caagttgcgg aagaattaaa
     2161 acaaaatccg ccaaaacaag tggtgacgcc tcgtaaagat gtcacacctg ctgaacctgc
     2221 gaagcaaaca aaaaaacaag tacaagcacc aaaaaacaat gcgaagaatg gtcggaatgc
     2281 tggtaaacag caaaagcgta aatagttatt tgtaactagc ttcttcgatc gacccagatc
     2341 atttctgatt ttatgacgat aaaagccaac gtactgtgaa atatagaaaa ctatatttcg
     2401 cggtacgttg gcttttttat acacggcctc tttatacatc cgtatttata tccagttgta
     2461 tcaccagtca agaagttgtg cttgattatt cttcttcatc gtctcccatg ccagcaatca
     2521 cttggttcac tgaaacgata ctctcacgac ccgaaccaat aaaatctggt tctgctccaa
     2581 tcatttgcga attgatgaat tcgatgacca tcggcaaatt catgccagca tatagttcga
     2641 tcgtttcacc ttccatgatt agcttggaaa cggtatttgc aggtgtccca cccaacagat
     2701 cgcaaagcac tacgtaatct ttcaatggtg ctgtaacgga gaggaatctt tctttatacg
     2761 tgtccgcgcc atcttcaggc aataaaggga tcgtatagat attttcttgt ggtcccatga
     2821 tcatttctgt actgtttttt agttcttcac aaaatttacc atgactaatc aagactagtt
     2881 gtctactcat ctttccaatt ctcccttatt tcgaaatcac acctaatgca gataatgcta
     2941 cacatacgat caatacgatg aatattgctc gagtaggtgt catatttttc ttacctaaca
     3001 tccaataaac gccaccaacg attccagctg gtaaaagttt aggtaagatc atatcgatat
     3061 agttttgcat attcaacgtt acatccccaa tcgttggtgc ccatgagaat ttaacattga
     3121 tcatggttgc tactaacgct ccaaccatga aaactcctag taaagtagcc gcatctgtca
     3181 atgcagtcaa gcgtgattgc atcgtggtaa ctagagaaat cccttctttg taggcaaatt
     3241 ctagttgttt ccaacgaaag accatgacag cgacctgagc aaggatccag ataaggatac
     3301 ctactggatt accatctgtt gccatatttg cggctaaagc tccaaatatg gtcgggatca
     3361 atgccgcaaa tacagaatca ccaattgcag cgaaggaacc catcaatcct gctttcatcc
     3421 cagaaacagt ttcctttgaa gagactcctt ctttttcttc gatagcaaga tcgatcccag
     3481 taatgatcgt gttaaagaaa ttagacgtat taaaaaattg cgtatgtgtt ttcatcattt
     3541 cttttaattc tggtgtatca tcaccataga tttttctaag ttgtggcaag atagtataca
     3601 gataacctga accttgcatt ctttcatagt tccaacctaa ttggaaagta aacaaacttc
     3661 tacgattgat ctgcttaaaa tcttctttgg tcaatttata attagatttc gtcatcttcg
     3721 atttccccct catcagattg tgattgctga cttactactg gatgttggtc attcttttga
     3781 tcattcttat aactgatcgt tgccagtgct agaccaatca atgccacagc aagcatcggt
     3841 aatgaattga atgttcctgg gaaatctttg accacgctag ccacacttgt tcctaacaat
     3901 tggacattcg agaacactgt tgctaataac gctgtgatcg taaatcctaa aatcagataa
     3961 gaaatgtgtt tctttactgg taggtagcgt agtaaaattg caaacccaac tgctggtaag
     4021 actgcccctg caacagataa gccatctcct agccatttta ggtcgccgtt taaaacagcc
     4081 acaacatttt ggactaagcc accaccgaat gctagggcca agaatacagg aaccattctg
     4141 gaaagtgccc aaggtaatgc ccccatcaag aagttccgtt caattgcttt gtaattcatt
     4201 gtttccacat gttgatcgat ccgatgtgca aaatacgtat tcgcaaaacg tgccaaaata
     4261 tctaattgga tcatcaaact tgctacagga accgcgatgg ccgcaatcgc ttgttctggg
     4321 ttcatcccta gagaaatgga gaaagctgtt gctaagatcg ttccagagtt ggcatcgatt
     4381 tttgatgcac caccaaatgt ccctacccct aaaacagtca actgcatact tccaccaatg
     4441 attaaacctg cttttaaatc acccattact aacccagcat ataatcctgc aaaaactggg
     4501 gcgctcattg aagaataaat ctgtaattca tctaaaattt gataccctgc atataatgtt
     4561 aataataata tctgccacca ttgaatatcc atgaatcatt cctcctattt gattaaaaga
     4621 ctcataaagt cttctgcttt gtcatttggc accatttgag cgatcaaatg aacgccaagc
     4681 tgatctaatt tcttgaagac ctctacatcc gcatctacga cattgattga ttttgtgatg
     4741 gaacgtgttt catctgtttg tgacatattg ccgacattta attctttgat cggtacacct
     4801 ttttctacta gcgctaagat acgatctggt tttttcgcta cgatcagcac tcgttgagaa
     4861 tcatatttac ctgctaaaat atttgcggct gctttttcaa taggtaaaat acttaatttg
     4921 actccagctg gcgttgccaa ctttaaccca cttttttcga tcgcatttgt tgctacctca
     4981 tcgtctacga ccatgatccg accaatattc aactttgtcg tccaaagatt tgccacttgt
     5041 ccatgtatca aacgtccatc aattcttact ccaataattg ccatcatcca ttctcctttt
     5101 ctaacttttt tctattggtt ctttgacaaa atctataact tcactgaatc taccttctgt
     5161 ttcaaagata acgatttcat tttgtccttt tctaaataaa ccctcgggaa caaacagtga
     5221 aagaattggt cctacttccc aaaaacgacc aatattcgtg ccattcaccc aaacgatccc
     5281 tttcccaaac tcactaaggt ccaaataagt atcaccgatt tctgacagtt ccgcttcaaa
     5341 gcggtaaaat gctggttgac ctgcttccca tttttttgaa aaatcgatgt gttctgtagt
     5401 ctctaaaggt aaacaatact gtgtccattc tgtgataaaa tgaagatcgc tcatcactcc
     5461 tgtacgtatt ccttttcttt gcgtatcagc tagcagcttg tgtccatagt tgacccgtcc
     5521 catattttca aataatagat caatttgatg gtcttctttc tcaagtgtca atggaatggt
     5581 ctcaccgatc tctgtttggt attgaatcgc ttgcttttct tcatcgagaa aaacatggac
     5641 ccgatcacgt ccgtcgatga tccgtaattt ttcattgacc gcatcttttt tgatcgttgt
     5701 tcgatacaat aggtacccag tattctgacc caattcttcc attgtttgag gataggtcga
     5761 gtggatcggc ttgctgatgg tatccagtgt tgcgaaaaag ctgacttttt ctttcaagtt
     5821 gattcctttt ttgacgattg tttcttttat ccatggttcc atttgctgca tttctggaaa
     5881 attttctgcc atcattcgtt tcagagcaaa atatttttcc gttggatttc cctgctcatc
     5941 caatggtgca tcgtaatcat aggaagtgac ttgcggcaaa tcaatcgcgc ctctggctga
     6001 acagccattc ataaacccaa aattggttcc gccatggaac atatagagat tgatactgcc
     6061 gatctgtaat gcttctttga ctgcatccgc taattcttcc ggatctcttt taatgatcgg
     6121 ctctttccaa cgattaaacc agccatccca aaattccata cacatcaacg gccatttttt
     6181 accatgtgct tcaaaaaact gggccatatc tgcaaaattt tcttttgctt ttgagccaaa
     6241 attccctgtc gtaaaaacat catcgtcaat caaaatgcct gctcttaatg tggcttgcca
     6301 aggtccatca gaagtgaata gcggtacatc gattccacct tcctccaagt atttttttac
     6361 ttggcgcagg tatgctttct cttcaccata cgaaccgtac tcattttcta cctgcaccat
     6421 taagacgttg ccaccatgag tgactagatg aggaacgatt ttaggtagta gcgtttggta
     6481 ataccgtttg acatacttca tgaagcgagg atccgctgaa cgaatacggc actcctttgt
     6541 caatagccaa gcaggcaagc caccaaattc ccattctgca caaatataag gggctggacg
     6601 tacgattgcg tataatccaa gggattgcgc taactctaga aaatgggaaa gatccaatat
     6661 tttcgaaaaa tcataggttc cttcttcttt ttcatggaga ttccaaggca cataggtttc
     6721 cacagtatta aatccaagcg ctttcaaatt gtgcaatgac cgctcccaat tcgtcggatg
     6781 cactcggaaa taatggatgg caccagataa aattttgaaa ggttgaccgt ctaacaaaaa
     6841 ctcttctttt atttcaaatc tagtcaagca aatttcctcc tcgtttttag aaaccgcttt
     6901 ctaattttat aatcaacgaa tctttctgac gttgatttcc cccctaaaat aatcgacttt
     6961 cacaggatcg atccatcctg ttttttcttg ttgtgcattc gccatcccac aggccatcgc
     7021 aaacgcaagt gtttcttcaa tcgtatagct ctttgataga gcatgtgcta agcctgcaat
     7081 agtcgaatct cctgacccca caggattcac caccgcgatt ttaggaatcg taacctgata
     7141 gaagtgtctc cctactttag cgattgctcc ttttgcgcct agcgaaagaa cgatccaatt
     7201 gattccttca aaaagcggat gatccaataa tgattggatt tcatctaacg aagtagccga
     7261 agaaattgtt tggtggacca actctcctaa ttcttccaaa ttaggtttga tcaaaaatgg
     7321 tttttccttg ccttccaaga cttcaagcaa ggtttgacca gaagtatcta acaatactcg
     7381 tgttcctttt ttattcgtta gggcaagtaa ctctgcataa taacttttag aaaatccttg
     7441 aggcagacta cccgaaatcg taaagatatc agttgtttcc attagtcgtg caaaatgctc
     7501 ggtaaattta tgttgctctt cttccgtcac aacaggaccg ctttccaata gttctgtttg
     7561 attcactcca tgtaaaatag caatcgaatc tctcgtttcc cctttgatag gtaaaaattg
     7621 atgcgttatc cctgattgat cgagttgttc tttgatatat gctccatgct gcccgccgac
     7681 taatccagta gctaaaacat catctgctaa taaatgaagg acacgagcaa cattcaagcc
     7741 ttttcctcca gccgttttgt tgaccgtttt gacccgattg acttggtcga tccgtaattc
     7801 atccaacgtg taagcgatgt caattgaagg attcatggta actgtcgtga tcattttgct
     7861 gtcaccgaat cttcttttac tttgtcaaac caaggggtgg cgttttcatt tatcacttta
     7921 ttcaacgatt cgatattttg tcgcccttgt gttctcaacc actcaatcgc accttctcta
     7981 ccattttcaa catatgcttt gactccatct ttccaagtcg cacgtccgca taacacaccg
     8041 ttgaatgttg aaccagattc atgagcaaag atcaatgttt gttggaacaa tgcagtcgaa
     8101 acccctgcac tcaagaagat aaatggtaac ggtgttgcat cactttgttc cttgaagaat
     8161 gccgctgctt cttttttact atacgcaatt tcaccatcag aataaccttc tacatagttc
     8221 atattgactg gcacttctac tttcaataca tccactttat atttaggatc agaaaattct
     8281 ttcatcatct cgatcacttt atgcggtttg atcttcgcat attctaatga agtgctgtca
     8341 ttggttttgg catcatacga aaccaattct agataaaacg gacgcttttc acctgcacat
     8401 tcactgccta aacgttcgat aaatacatgt ttcaaatgat tgatctctgg gtcttcatct
     8461 acatcataat aaagcaaaaa cttgatcgca tcggctgttt gttcctttaa tctaagcgta
     8521 gaccaatccg ctaataaatc aggcagacgt cctggagttg tcgcatcata acccgttttt
     8581 tcataggcta aaagtagtcc tgcttcctga tgtctggctt tagcagctgg caatccatac
     8641 tctggatcta gtaaaatagc agaagcataa ggcgttaatt ctgctgatac taattctttg
     8701 aattcagtga tctgttgcgc cgttggttct tttcctaacg ctttgatcat ctttttcaaa
     8761 gcaccgcgtt gatcaattgc caatgcactg atgattcctt cttttgttga taaactcttc
     8821 atagctgcaa ttttttcttc attcatcctt cgtttcccct ttgttcacat attcatggat
     8881 aatgacacct ttgaccactc gattgactgt acctgtaggc gatggtgtat ctggtttatt
     8941 cgctactttt atcgacgtat cgagcgcaat gatttgggct gtcatgatca taggtaaggc
     9001 aagatacgca tcaggtaggc tgatcgaagc ttctaacaaa agacttgtac ctgtgaaatc
     9061 gatgtttcct ggttgggcga tcgcgatgac actcgtggta atttggtcat cttttatttc
     9121 ttctagaata tctaagtcat aatttcttgt atacggttgg ttattaacaa aaccaataac
     9181 aagtgtttcg ctatttaaaa acgattttgg tccatggcgg aagcccatag aagaatcaaa
     9241 gatcgttgcc actttaccgg ctgtcaattc caatattttc aattgtgctt ctctcgttgc
     9301 accagccaaa cttcctgaac caagataggc gatacgttca aatgacttag gcaataattt
     9361 ttggatttct tgatctcgtg aaattagatc ttctccagtt ctgatcaatt gttgaagata
     9421 actttctttt ttctcaacgg cagtttggtc gaatacccat aacgcactca acaacatgca
     9481 cgtaaaactt cctgtcatgg caaatcctgc atcatttgat ttttctggca tcagtaaaag
     9541 ataatttcgt tcatctgtcg ctgcattctt tgctaactga ccttctttag cacaagtaat
     9601 cgtcaaatga aacaatgtct tgatttgttc attggcaatc ttgactgttg ccacactttc
     9661 tggactattt ccactacgtg caaatgacac cagtaatgtc ggttcatccg gaaatagaaa
     9721 ttcttttggt gaagacacga tatctgtcgt accaaagcta tgaaaaataa agtgatcgtt
     9781 atcaccgtat tcatttaaat aaggtactgc tgtatccccg acatattgtg atgtgcctgc
     9841 accagtaaaa atcactcgaa ctcttttgcc atttgctgct ttttttatct ggtcaaaaaa
     9901 caatgctaat ttttcttgat tctgtgctaa tatctcagct gtttctttcc ataattgcgg
     9961 ttgctgtttg atttctcgcg tagttatttc cgcacccaac tcttttaatt tatccttcga
    10021 tagttcaaac atgttgtttt ccctctcaat cattttcgga cgtggcggat cttataatga
    10081 aactggtctg ctctcgctac actatccgta tattcaataa tttcattttt cacattatag
    10141 gtcgttcggc ttagatataa taccggggca ccatcagcga tacgaagcgg atcagcatct
    10201 ttcttttgaa cgatacttgc ataaaattct tcatctgcta attggacttg ctgttcataa
    10261 tcctgagaaa acacatcata caatggctta ctttcaagta atggcagatc aagaccaata
    10321 aatagttttg caggtaaata agaacgttcc accatcaatg gttcgtcatc cgctaaacga
    10381 agtcttttca atttgaaaac tgtttcgcct aaattgatgt ttagattctt ggcaaccttt
    10441 ttgtttgctt ccatttgctt gaattccaag atgactgtat taggcacccg tcccaattcc
    10501 ctcatctctt ctgtaaaact atacgctccc gccagatcag acgacttatc tgatagatca
    10561 gacacatatg ttccttttcc gtgtcggcga taaatatacc cttgcgtctc taattcttgc
    10621 aaagcaaggc gaaccgttgt ccgactcact ccataatgct gacaaagttc tctttcagat
    10681 aacaatttgt catgaggtgt catctgtgtt tcgatacgtt ctttcaatac gtcaactagt
    10741 tgataatata atgattttcc tttacttgag ctctccataa aaatacctct tctttactgg
    10801 ttataaccac aacacaagaa tacccacata ttcctcattt gtcaaagggt ttttggcgta
    10861 aaaaagctat tataacaagg tttcaaggta atttagaact ggtatcaaaa gatacctcaa
    10921 aaataccagt tgacaaataa aaaacaaaaa aaagctgaat cttggcaaag attcagctaa
    10981 atggtctaga gaagtaaaga aaaaatattc tttatacatc tgtggctatt tctactatcg
    11041 acaagcaaat gggcctaata gactactatg taaataaaaa tggctttctc taaataaagt
    11101 aggatttttt attttttaga taacttttct ctcatgccgg tctgatagtc gaaattttgt
    11161 tgtacatttt taaaaataac aaaaattttt tatgataatt ctgtcttttt ctagtttcct
    11221 tatggcgtaa
//
LOCUS       DAYYAG010000090        11137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000090 DAYYAG010000000
VERSION     DAYYAG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.091"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(546..1112)
                     /locus_tag="ACHARQ_002072"
     CDS             complement(546..1112)
                     /locus_tag="ACHARQ_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8410473.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1112..2068)
                     /locus_tag="ACHARQ_002073"
     CDS             complement(1112..2068)
                     /locus_tag="ACHARQ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8410474.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2068..2724)
                     /locus_tag="ACHARQ_002074"
     CDS             complement(2068..2724)
                     /locus_tag="ACHARQ_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8410475.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3094..5112
                     /locus_tag="ACHARQ_002075"
     CDS             3094..5112
                     /locus_tag="ACHARQ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8410476.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5182..5388
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5539..7953
                     /gene="leuS"
                     /locus_tag="ACHARQ_002076"
     CDS             5539..7953
                     /gene="leuS"
                     /locus_tag="ACHARQ_002076"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8410477.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8301..9854
                     /locus_tag="ACHARQ_002077"
     CDS             8301..9854
                     /locus_tag="ACHARQ_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8410478.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10235..>11137)
                     /locus_tag="ACHARQ_002078"
     CDS             complement(10235..>11137)
                     /locus_tag="ACHARQ_002078"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410479.1"
                     /translation="LQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILDETDE
                     IPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGAGKTT
                     LVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIAYGKM
                     DATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILANPPV
                     VILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGDIVEK
                     GTHSELLQSPTLYASLYNSQFQIA"
BASE COUNT         3628 a         2077 c         2214 g         3218 t
ORIGIN      
        1 aatatagcta agattgaaac atccatctgg aatttatctt ttttactgct ttgatcataa
       61 aagtctgcat attcatcact gatatttttt ctaataaatt cttccatcat aaaaatcacc
      121 tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg atatttgagg ggcaaaaaag
      181 gggcataatt tgactgatat taagttagct attgatatat gaatgacata caaaaatata
      241 aaatctttta aaaatgctta tatatcaatg tttttgccat tcttttatat atctgttata
      301 tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata aagaggttgt gaaaaagctg
      361 tcctgcatca agtaataaga acagccaatc aaaaacagct tctcatgttt ttgatttggt
      421 tggacttatt accgcagata taagcttttg agcaccgtga ataaagaggt tatgaaatca
      481 acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat atcatttatg ttttgcgttt
      541 tttatttatc tttttttctc aatacaatat aaaataggcg gctggttctt ttggttgata
      601 aaccgataat tcagcacatt atatgtttcc tgtggcagtt tggaacaaaa agaatcaacc
      661 atatccagtt ctttttctcc accttcatga ccatagtaga cgacaaggat caatcgtccg
      721 cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc gtgtagtctc tggcatagtg
      781 atgattgctt tatcactttt tgggagatac cctagattga aaatccctgc tttgagattt
      841 tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt gtagaaacaa tgagacacgt
      901 tcttctaact gacgctgttc tagcctttca cgagtagcat cgattgcttg ttgctggata
      961 tcaaaactat agacatgacc agtttttccg atgtgttctg ctagaaaaac cgtatcgtgg
     1021 ccattgccca tcgttgcatc tacgacgtga tcccctggtt gcaagatttc ttgcagtaat
     1081 gtatggctga aatgcaaagc agtttgcaac atcagacgct cacctttttc cgtacatcat
     1141 attttccttg gaagctatct ctgcgaagaa gttcttcatc aattgcattc aagacttccc
     1201 attttttcaa gctccacatt ggtccaatca aggaatccca cggcgcatcc cctgtcaaac
     1261 gatggatgat gatctctttt ggtatcattt ccagctggtc gcaaatgaca ttgacatagt
     1321 caggacggct cattaattga aggcgacctt catgatagtc ccgcaacatt ctcgttttgc
     1381 gcatcagatg catcaaatgg agcttgattc cttggatatc tgaatctaaa atggtgcgtc
     1441 tgacattttc tttcatcatt tctaaacttt cgcctggtaa tccgttgatc aaatgcgtac
     1501 aaacacggat attatgtttg cgtaatttag ccacgccatc taaataagtt tgataatcat
     1561 gtgctcggtt gatcagtttt gaagtttctt caaaagttgt ttgcaatcca agttctaccc
     1621 ataaatacat ccgctcgttc agttcagcaa ggtaatcaac tacttcatct ggcagacaat
     1681 ccggtcgtgt accgactgat aatcctacga cacctggtaa attgactact tgctcaaatc
     1741 gttctttgat tatttcaact ggcgcatggg tgtttgtaaa attttggaaa taaacgatgt
     1801 attgttggac atgaggccat ttttgatgca tcatgtcgat ttctttctgg aattggattg
     1861 gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc agatacactg caaaaagtac
     1921 aaccgccatg ggcaacagtc ccatctcgat tcggacagtc aaatcctcca tcgatcggca
     1981 ccttgaaaat tttcccgcca aactcatttc gtaaagcgta attccaagaa tgatatcttt
     2041 tatttccttc tgaataagga aaagtcatta aaagtccttc tttctgtaag agttatctat
     2101 ttgttttctt ttttctctat acactggata agtcaggcac agccaagaaa gactgagtaa
     2161 gtaaccgccg atgatatcag taggaaaatg aacgcccaga taaatgcggc ttatcccgac
     2221 acttactata ataaacatca tcagcaattg aagcaatgtg cgtaaggttc ttttttgaac
     2281 aaaagctgga agcagaaata gtaaagaacc gtaaagaatc atactgcctg ctgcatgtcc
     2341 actagggaaa ctgtaacttg tttcaactac taagtgcgcc aatgaaggac gctctcgcat
     2401 gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt acaataaatc cagaaacgaa
     2461 ccaaaatgtt tctgcccttt ttttcttata ccacatccat aaagccgcac aagcaaataa
     2521 aatgatgacg gtaagaggat taccgaattt agtcataaag agttggtagc tgttcagatc
     2581 aggataagaa gctctgacaa tgcctgtaaa ccactgatcg aaaccagaaa gccacgacag
     2641 atggaacttt acgatccagc ccaaaatgaa aaacaggaca agaaagaagc taccagccac
     2701 ctgaaatgat gttttatatt tcatacaatt cctcctttga agcaatgact tattatagca
     2761 tttttggtcc agcgaaaaaa acggtaaaga gataaaacca atgtatgatt cctctttgaa
     2821 taaccaatat tcttataaaa aaaataactg taatcggaaa gacatttttt ggtaatgcaa
     2881 atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc cttttaaaca agaacgtaga
     2941 cgtaggaaga acttgaaaat gaacagaagt tgttcattac ggtaaattac aatatgatta
     3001 caaaaaacaa caaagctctt aaataacttt taataaatat taagagaagt tataattatt
     3061 aataatagat aaattgaagt tggaggatag aagatggaaa acattgcaag aaaagaacgg
     3121 agacgtttag aacagaccaa acgttttcga aaagcaaaac gaggggcagc aatcgtggga
     3181 actgcgatgg taggctgttc tgtcgctgcc ccattagtac agccggttcc tgttcaagcg
     3241 gatgaaacac ctgttcagtt cggcgcaaga atcaatacag cggcatttat cacagaaatt
     3301 gctacttatg ctcaaccaat cgctcaagcc aacgatctat atgcatcagt gatgatcgca
     3361 caagcagttg tagaaagcgg ttggggcagt agtgcgttat ctcaagcacc ttattataat
     3421 ctttttggga taaaaggcag ctatcaagga caaaccgtct acatggatac gcttgaattt
     3481 ttaaataatc aatgggtaaa taaaaaggag cctttccgac aatatccttc ttttgctgaa
     3541 tctttcaatg acaatgccta tgtacttaga aatacttcat ttggcaacgg ttattactat
     3601 gccggtactt ggaagagcaa cacgaaatcc tatacagatg cgacagcttg tttgacaggg
     3661 cgttatgcga ctgatcctgg ttacgcaggc aaattaaata atatcatcgc aacttacgga
     3721 ttgacccaat atgatacacc agctagcgga aacgcaggcg gtggagcgac tacaggtggc
     3781 aataatactg gaagtgcagg tagtactggc ggttcaagca attctggtaa tacaaactca
     3841 ggttcagcaa cttcgaccac ttatactgtc caatctggtg attctgtttg gggaatctca
     3901 aataaattcg gtatcacaat ggatcaattg atccaatgga acaacatcca aaacaatttc
     3961 atttatccag gacaaaaact gacaatcaaa ggtagccagg aaaatggctc ttcgacgaat
     4021 aattccggta acaataccaa ttcttcagga aatgctggta catcaaacgg aggacaaaca
     4081 actggtgcga aatacactgt tcaatctggt gattctgttt ggaagatctc aaatgatcac
     4141 ggaattacga tggctcaatt gattgaatgg aacaatatca agaacaattt cgtttatcca
     4201 ggacaacagc taatcgtatc aaatggcggt tcttctaatg ctggagcaaa tactggctca
     4261 aatactggga atacaagtgg ctctagttcg tccagctcaa gttcttctaa tacagcttca
     4321 ggaacgaaat atacagttaa agccggagac tctgtttgga gcgtctcaaa taaatacgga
     4381 attacgatgg ctcagctaat ccaatggaac aatatccaaa ataactttat ctacccagga
     4441 caacaactga ctgtttcaaa cagtggatca acaaacagca cgtcaggtaa tacaggttcg
     4501 tcaaccaata caaataacag cagttcagtt aatactggca gcagttcgtc tcaaggcact
     4561 tataccgtga aagcaggtga atctgtttgg agcgtggcaa ataaaaacgg catcacgatg
     4621 gatcagttaa tcgaatggaa taatatcaaa aacaacttca tttatccagg acagcaattg
     4681 atcgttaaag gtggaacttc ggcaaataca aatacaggtt caactacttc tgcagcgaaa
     4741 ccaaatacac cgaatacgtc tgctacgaca agtacatcat ccactggtaa tacgatgtat
     4801 accgtgaaag cgggcgagtc agtttggagc gtagcgaaca aacatcatat cacgatggat
     4861 cagttaatcg aatggaataa tatcaaaaat aatttcatct atccaggaca gcaagtgatt
     4921 gtgaagaaag gttcatctca atcaacaaac caacaatcag ttactggttc gaaaacctat
     4981 actgtgaaat ctggagaatc tgtttggagt gttgctgaca gtcatggaat tacaatggct
     5041 caactaatcg aatggaacaa tattaagaac aactttattt atccaggtca aacgttgatt
     5101 gtagctaaat aaaatagctg ttcgaagaaa taacgattga attccacaaa aatatttgtt
     5161 actataaggc tacttaaata tgacaacact tgaaaaggaa gtagtaaaaa gactgaagtt
     5221 tcagagagcg catggctggt gagaatgtgc acggatgctt ttgaactcgc ctttgagttg
     5281 cccttgctga atacccgagt aagcaagcgg cggtgacggc cgttacaacg ttcgaggcct
     5341 tttcactaag gtaaaattag gtggtaccgc gtagattacg tcctataaaa agagcaatct
     5401 ttttatagga tttttttatt tcttttgtga agtttgagaa aacaaagcaa aaggaaataa
     5461 aaataaacag attctgccgg ttttttcgtt atatttataa tagaaacaag tgttttcaat
     5521 agaacaggag gacagcaaat gagttacaat cataaagaaa ttgagaaaaa atggcaaaaa
     5581 tactgggcaa aaacgaatac tttcaacacc catgatgatc ctgaaaagcc aaaattttac
     5641 gcactggata tgtttcccta tccatccggt caagggctac atgtagggca tcctgaaggt
     5701 tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc aaggatacaa cgtgctgcat
     5761 ccaatggggt gggatgcatt cggtctgcct gctgaacaat atgcgctaga tacaggaaac
     5821 gatccagctg agtttaccca aaaaaatatc gaaacattcc gacgccaaat caattcttta
     5881 ggatttagtt atgactggaa tcgcgaagtg aatacaactg atccggagta ttataaatgg
     5941 acacaatgga tctttacgaa gctttatgaa aaaggtctag cttatgaagc agaagtagcg
     6001 gtaaactggg tacctgaatt aggaacagtt atttcaaatg aagaagtgat cgatgggaaa
     6061 agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc gccaatggat gctgaaaatc
     6121 actgcttatg ctgatcgctt attggatgat ctagaattag ttgattggcc agaaagcatt
     6181 aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag caaatattga atttaaagtg
     6241 gctggtacgg ataaatccta taccgtattt acgacacgac cagatactct attcggtgca
     6301 acttactcgg ttcttgcacc tgaactagat cttgttagag aaattacgac ccctgaacaa
     6361 aaagctgctg tagaagctta tatcgaagag acagctaaaa aatcagatct aaagagaacg
     6421 gatcttgcaa aagaaaaaac aggagtattt actggtgctt atgcaatcaa tccagttaat
     6481 ggcaaggaaa tcccaatctg gatcgccgat tatgtattat cttcatacgg cacaggagcc
     6541 atcatggctg ttccagcaca tgatgaacgt gactttgaat tcgctcaaac tttcggacta
     6601 gaaattcttc cagttatcga aggtggagat gttgaaaaag ctgcttacac agaagatggt
     6661 acgcacatca actctgaatt tctagacggc atgaacaaac aagaagcaat cgataaaatg
     6721 aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag tcagctatcg cttacgtgac
     6781 tggctattct cccgtcaacg ttactggggt gaaccaatcc caatcatcca ttgggaggac
     6841 ggaacagtca cagctgtccc agaagaagag ctaccattac ggttgcctaa gacaaaaaac
     6901 atcaagccaa gcggtactgg agaatctcca ttagcaaata tcgaggagtg ggtcaatgtc
     6961 gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga atacaatgcc acaatgggca
     7021 ggaagctcat ggtattacct gcgttatatc gaccctcata acaaaaaaga actagcagat
     7081 tatgaaaagt tgaagcgttg gctaccagtt gacatctaca ttggtggagc agaacatgcg
     7141 gtacttcatt tactatatgc acgattctgg cataaattct tatacgatat cggtgttgtt
     7201 cctacaaaag aaccattcca aaaactatac aatcaaggaa tgatcttagg cgaaaacaac
     7261 gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg atgatgtggt cgaaacttac
     7321 ggtgcagata cactgcgcat gtacgaaatg ttcatggggc cattagatgc ttcgattgct
     7381 tggagcgaaa atggcttaga aggaagccgt aaattcttgg atcgtgtatg gcgtttgatc
     7441 gttgatgaga acaataaaat gcgtgaccgt atcactacat tgaatgatgg caagcttgat
     7501 aaagtgtatc atcaaacagt gaaaaaagtg acagaagact atgaaaatct tcatttcaat
     7561 acagcaatct cccaattgat ggtctttatc aatgaagcct ataaagtgga cgctctgcct
     7621 tatgaatata tcgaaggatt tgttcaacta ctcgcaccga ttgctcctca catgggtgaa
     7681 gaactatggg caatccttgg aaatgatggt ggtatttctt acgcaccatg gccaacatac
     7741 gatgaagcag cacttgttga agacgaagta gaagttgttt tccaagtaaa cggtaaagtt
     7801 cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg agctagaaaa agcagcatta
     7861 gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag tcagaaaagt tattgcggta
     7921 ccaaacaagt tagtcaatat cgttgctaat taattggctc tttgtcaata aggactgatg
     7981 agttgtgcaa aatcaaatct gggccagaat gaacttcatt ctgtctcaga ttttttgtta
     8041 tttcaaattg attaattgat tgatcaaaaa ggttttgatt ttcatgttct caaacacgtt
     8101 caatgtcttt ttcttctgaa tggtcttgta agagatctct gtaatcatca aatttttgtc
     8161 tattaatctt agtatttttt gatgtataaa ctacctgaaa ttgagagtac ataccaaatt
     8221 cataaatttt ttattagtgg gactagtgat tgtatggatt ttgacgcata atgaatgaag
     8281 tatgagaaaa ggagagacaa atggatcaag aactatttat tatcgaagta ttatcagcta
     8341 aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag aataatgttc aagcttttaa
     8401 gcttagtatt tttctctggt ttgctttttt tcggagctgt atcgttccta tatcgccata
     8461 tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt aaataatttg tatcactttt
     8521 cgtggggagt tggcggaata acaattgttg tgcttattgt ttttcttcta cttatttggc
     8581 tccgttacca tcaattgtcg cgtcatttcg atcaatggct tagtcaaaga gcacaacagt
     8641 ttttacataa taaaccaact tctcaagatg atgactatct ttattttgag aagacttcga
     8701 cgagtcaagc ttttcggata caaaaaagtg attgccagtt attgctgaga tcacttgatg
     8761 atttacctat ctacttaggc gaaagcaaat caaaattcgg aatgaacaca ttccatattt
     8821 ttttactaga aaacgaaccc agatgggaaa aaagccagtc ttcgcctggt ggatacctga
     8881 caactaagat gagtacatta gtcactggtt ttttcctcat tcttttagct ggaacaatct
     8941 attatgggag atacactcag ccaaaaatgt cttctcatat aaatgctgct tctcacatga
     9001 caatgaactc aactaacgat acgcatatac aaaagacaaa tgaagggttg ttagctgatc
     9061 ctaagcaaaa ggtaaatttg acaacacaaa agataaatga tctcaaactt gatacagaga
     9121 cgcactctct ctatatgaca acagatagtg gtgctaattg ggtatttgtg ccgattgatt
     9181 cgcaatggtt gcgttttggc gaatacaccc tgacaacggg gctgattcct gaaggttatt
     9241 ggatggacaa cacatatgat atttccgccg atttctcatg gtttatctat tcttctgggg
     9301 atgatgtacg tctgctgact tcaagagata atggtaaaac atggcaaacg tctattatca
     9361 cccaatcagt tcagccgata cgttatcgaa aagcggtatt ttatggcaac gatggtctat
     9421 ttatctgtac atcagccagt gggatgtctg cagaaacttt gtacatttac actactatgg
     9481 atggcggaat cacttggata aaaagtggaa gtacaatgat caatcagcca gtacaaaatg
     9541 tcagcttcgt tacaacttct ttaggttttg tttcgacacg tgaaagtttg ttttatacaa
     9601 acaacggagg acgctcattc aaagaatcag ttctgtcgat tccggaaaac tatcagacag
     9661 gggggataga tctttttcaa tcaccaaatg aagttactca aataagcgcg aatacgttag
     9721 agacaaaatt ttatttgttg aaaaaaggac caatcgatgc agggaaaatg tttgcctgcc
     9781 tcttccgttc aactgataac ggggaaacat ggcaattcgt tcagcaacta tcgcaagtag
     9841 aacaaggaga ttaaagaaga gagattgtga gcctgacgtt cagcaccgag tcacaagaaa
     9901 caaattcaaa aaacagcttc tcatatttgt tgaatagtgt aactcgtgat tcggagttga
     9961 caagagcaac aagaagcgat tttgaagtat agcttctcgt attttacaag atcgtgtctt
    10021 attgccgata ggttgtctct tgaacaccgt ttattcgtaa aaagggattg tgacaagtct
    10081 tttgtcacta cttaccgaac gaaactgaag gagctatcca aaaagtatca ttagtattat
    10141 tctttgtgtt tctagtggca ccactagtac atttaaaaaa gctatcgatc atggactaat
    10201 catacatgat cgatagcttt ttagataatg tttgttacgc tatctgaaat tgactattat
    10261 aaagactggc ataaagcgtt ggtgattgga gtagttcaga atgtgttcct ttttcgacga
    10321 tatcgccatt cttcattaca aggatcatgt ctgcattttc aatcgttgac aaacggtgtg
    10381 caatcacaaa acttgtccta tcttttgtca cagcttccat tgcttgctga atcaatgctt
    10441 ctgttcgtgt atctacgcta gaagttgctt catctaaaat aacgacagga ggattcgcta
    10501 gaatgactcg agcaattgtc aataattgtt gctgtccttg agataacgtg ccgttttcgc
    10561 tagaaatgat cgtatcatat ccgtctggca gtgttcgaat aaagtgatca cattgtgcga
    10621 tttcagccgc ttcgataatt tgttctcttg tggcatccat tttcccgtag gcgatattat
    10681 cagctaccgt tccttcaaac agccaggtat tttgtaaaac catcccaaaa gaacgacgta
    10741 gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa ttcgatcttt cctttattca
    10801 gttcataaaa acgcatcagc agattgacta aagtcgtttt tcctgcacca gttggaccaa
    10861 cgatcgctac cgtttgtttt ggctttactg aaaaatcgac ttgattcatc aatattttat
    10921 ctgatgtgta accaaactgt acatttttga aagctatttt tcctttcggt tgttctaaaa
    10981 gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc taatatctca aatatcctat
    11041 cgattgctgc cattgcggat tggatcgagt tgatcacata agaagcggta gaaattggtt
    11101 cagaaatctg attgatgtac tgaagataag cttgtaa
//
LOCUS       DAYYAG010000091        11089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000091 DAYYAG010000000
VERSION     DAYYAG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.092"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1310
                     /gene="lysA"
                     /locus_tag="ACHARQ_002079"
     CDS             51..1310
                     /gene="lysA"
                     /locus_tag="ACHARQ_002079"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8410480.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1297..2097
                     /gene="dapF"
                     /locus_tag="ACHARQ_002080"
     CDS             1297..2097
                     /gene="dapF"
                     /locus_tag="ACHARQ_002080"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8410481.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2100..2981
                     /gene="dapA"
                     /locus_tag="ACHARQ_002081"
     CDS             2100..2981
                     /gene="dapA"
                     /locus_tag="ACHARQ_002081"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8410482.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2978..3694
                     /gene="dapB"
                     /locus_tag="ACHARQ_002082"
     CDS             2978..3694
                     /gene="dapB"
                     /locus_tag="ACHARQ_002082"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8410483.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3700..4395
                     /gene="dapD"
                     /locus_tag="ACHARQ_002083"
     CDS             3700..4395
                     /gene="dapD"
                     /locus_tag="ACHARQ_002083"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8410484.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4411..5412
                     /locus_tag="ACHARQ_002084"
     CDS             4411..5412
                     /locus_tag="ACHARQ_002084"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8410485.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5409..6770
                     /locus_tag="ACHARQ_002085"
     CDS             5409..6770
                     /locus_tag="ACHARQ_002085"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8410486.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6730..7881
                     /locus_tag="ACHARQ_002086"
     CDS             6730..7881
                     /locus_tag="ACHARQ_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8410487.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7856..9025
                     /locus_tag="ACHARQ_002087"
     CDS             7856..9025
                     /locus_tag="ACHARQ_002087"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8410488.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9200..10579
                     /locus_tag="ACHARQ_002088"
     CDS             9200..10579
                     /locus_tag="ACHARQ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8410489.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10860..11089
                     /locus_tag="ACHARQ_002089"
                     /pseudo
     CDS             10860..11089
                     /locus_tag="ACHARQ_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3688 a         1940 c         2493 g         2968 t
ORIGIN      
        1 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
       61 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
      121 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
      181 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
      241 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
      301 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
      361 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
      421 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
      481 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
      541 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
      601 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
      661 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
      721 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
      781 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
      841 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
      901 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
      961 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     1021 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     1081 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     1141 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     1201 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     1261 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     1321 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     1381 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     1441 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     1501 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     1561 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     1621 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     1681 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     1741 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     1801 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     1861 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     1921 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     1981 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     2041 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     2101 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     2161 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     2221 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     2281 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     2341 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     2401 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     2461 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     2521 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     2581 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     2641 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     2701 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     2761 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     2821 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     2881 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     2941 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     3001 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     3061 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     3121 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     3181 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     3241 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     3301 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     3361 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     3421 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     3481 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     3541 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     3601 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     3661 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     3721 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     3781 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     3841 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     3901 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     3961 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     4021 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     4081 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     4141 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     4201 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     4261 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     4321 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     4381 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     4441 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     4501 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     4561 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     4621 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     4681 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     4741 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     4801 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     4861 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     4921 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     4981 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     5041 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     5101 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     5161 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     5221 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     5281 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     5341 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     5401 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     5461 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     5521 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     5581 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     5641 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     5701 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     5761 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     5821 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     5881 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     5941 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     6001 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     6061 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     6121 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     6181 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     6241 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     6301 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     6361 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     6421 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     6481 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     6541 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     6601 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     6661 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     6721 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     6781 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     6841 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     6901 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     6961 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     7021 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     7081 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     7141 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     7201 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     7261 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     7321 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     7381 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     7441 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     7501 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     7561 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     7621 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
     7681 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
     7741 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
     7801 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
     7861 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
     7921 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
     7981 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
     8041 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
     8101 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
     8161 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
     8221 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
     8281 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
     8341 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
     8401 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
     8461 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
     8521 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
     8581 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
     8641 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
     8701 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
     8761 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
     8821 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
     8881 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
     8941 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
     9001 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
     9061 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
     9121 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
     9181 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
     9241 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
     9301 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
     9361 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
     9421 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
     9481 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
     9541 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
     9601 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
     9661 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
     9721 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
     9781 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
     9841 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
     9901 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
     9961 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    10021 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    10081 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    10141 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    10201 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    10261 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    10321 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    10381 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    10441 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    10501 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    10561 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    10621 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    10681 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    10741 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    10801 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    10861 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    10921 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    10981 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    11041 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAYYAG010000092        10746 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000092 DAYYAG010000000
VERSION     DAYYAG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.093"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(265..1113)
                     /locus_tag="ACHARQ_002090"
     CDS             complement(265..1113)
                     /locus_tag="ACHARQ_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8410490.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1277..2179
                     /gene="gnd"
                     /locus_tag="ACHARQ_002091"
     CDS             1277..2179
                     /gene="gnd"
                     /locus_tag="ACHARQ_002091"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8410491.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2181..3182
                     /locus_tag="ACHARQ_002092"
     CDS             2181..3182
                     /locus_tag="ACHARQ_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8410492.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3179..3463
                     /locus_tag="ACHARQ_002093"
     CDS             3179..3463
                     /locus_tag="ACHARQ_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8410493.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3496..4293
                     /locus_tag="ACHARQ_002094"
     CDS             3496..4293
                     /locus_tag="ACHARQ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8410494.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4307..5140
                     /locus_tag="ACHARQ_002095"
     CDS             4307..5140
                     /locus_tag="ACHARQ_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8410495.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5158..6015
                     /locus_tag="ACHARQ_002096"
     CDS             5158..6015
                     /locus_tag="ACHARQ_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8410496.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6037..6546
                     /locus_tag="ACHARQ_002097"
     CDS             6037..6546
                     /locus_tag="ACHARQ_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8410497.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6583..6984
                     /locus_tag="ACHARQ_002098"
     CDS             6583..6984
                     /locus_tag="ACHARQ_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410498.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7096..7449
                     /locus_tag="ACHARQ_002099"
     CDS             7096..7449
                     /locus_tag="ACHARQ_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410499.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7483..7926
                     /locus_tag="ACHARQ_002100"
     CDS             7483..7926
                     /locus_tag="ACHARQ_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8410500.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7943..9028
                     /gene="uxuA"
                     /locus_tag="ACHARQ_002101"
     CDS             7943..9028
                     /gene="uxuA"
                     /locus_tag="ACHARQ_002101"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8410501.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9021..9734
                     /locus_tag="ACHARQ_002102"
     CDS             9021..9734
                     /locus_tag="ACHARQ_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8410502.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9941..10090)
                     /locus_tag="ACHARQ_002103"
     CDS             complement(9941..10090)
                     /locus_tag="ACHARQ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410503.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10485..10685
                     /locus_tag="ACHARQ_002104"
     CDS             10485..10685
                     /locus_tag="ACHARQ_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8410504.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3601 a         1695 c         2320 g         3130 t
ORIGIN      
        1 ttaatttttt agaaaataag aaacaaaaat aataaaaaaa agaaaagata aattttttac
       61 tactgtatac taactttgtt atcgcatcta acaagtaact cgaataatac caatacaacc
      121 ttctccaaag atgcgatgac atacgttcta acccaccgac tattgatgtt ctgtaccacc
      181 cttgcagaac caaaaaagcc actaccaaat atttggtagt ggcttttttt gttttatgga
      241 tttttcgctt tttctgttat ggtgctattc tttgatttca gaaatagtat gtcgaacttt
      301 agcgatagaa cgacgtgatt ctttttcgtt gtaaaacatc aaaatcacat atagagagtc
      361 caaaatgctt agttgagaaa tacgggaagc taaagcttca gatcggaatt ctgtttcttc
      421 agaaatagaa ataaaaacga catcgccaag tttagcaagg gaagaattag cttggctagt
      481 aataacgatt gtttttgctc cagtttttct tacggtttca gcaatatgga ttgtttcttt
      541 tgattgccct gtgtgagaaa tcaaaacagc acagtctttt tccgtcaata aagaagcttc
      601 catcaattga atatggtaat ccgtgctgtg agaagttgcg ataggggaac gcaaaaattt
      661 atgataagcg tcagttgcta caatctctga accgccaaca ccgaaaaaat agacacgttt
      721 agattcattg ataatagtag ccgctttttt tagatccttt tgttgtaaca acttttttgt
      781 gtcatttaaa gttgaaatac tagactcgaa caccttttgc gccatagtga gctcagtatc
      841 tgttttctga atattttcgt ggatagaaat agcagataga tcattttctt ggatcaacat
      901 ggccatttta aaatctttaa atccattata accaagtttt tttgtaaatt gaaaaaaagt
      961 agagtcagca atgcccagtt cacttgctaa attgctaata ggactatgac ttactttgct
     1021 tggattttct aaaatataat cggcgatagc ctgttctttt gggctgaagt cgttgtacat
     1081 tgttttgatt tgtagtctta tggagacgct catgagaatt cctcattttc ataataagat
     1141 tatcattttt tccgttctca ttaatgttaa agatacaagg agagtaggaa aaaatcaaac
     1201 aaaaaatatt tttgtaaaat ctatttacaa aaaaattttt ttgtataatg agggtataag
     1261 aaatggaggc gctatcatgg aaattggtgt aattggtttg ggaaaaatgg gattgaattt
     1321 agctttaaac ttaaaagagc accatcacaa agtacaagga ctcgacattt caagtacagc
     1381 tgttagtcag gcaagagaaa atgacattcc tgcttatcaa gagatctctg aatttctagc
     1441 tacttttgag cagaaaaaaa tcatctggtt aatgcttcct gctggagaga taaccgaaaa
     1501 catgttaaga aaactttttc caaaactcgg accaggagac attattattg aaggcggaaa
     1561 ctcttattat aaagattcaa tccgtcgagc agaagagttt cagaaaaatg aaatcggtta
     1621 cttggattgt gggacttctg gaggaatcga aggagcacga catcaagcct gcttaatgat
     1681 tggaggagat aaagaaactt ttctatccgt ggaacaatta ttcaaagatg tagcaattga
     1741 aagaggatat ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa tgatccataa
     1801 tggaatcgaa tatggaatga tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag
     1861 tgagtatgct tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg
     1921 cggctggctg atggaaatca tggaagatca gttaaacgaa catcccaatt taagtaatta
     1981 tcggggaatc gtttctgcat caggtgaagc aaaatggacg gttgaaacag cgctagaaat
     2041 ggctgttcca gttccagtaa ttgcaactag tttgttcatg aggaatcttt cacaggaaga
     2101 agatagtttt tcagcaaaag tagtttcagc actaagaaat gggtttggcg gacatgaaat
     2161 agttgaaaag ggtgagtaaa atgccagatt atcatatagc aatcgttaat tccagcagtt
     2221 tcggaagagt ctttccacac cacttggaaa gattaaagtc agttggagac gttcatttct
     2281 ttactgttga tcaagagatt ccaggaaaag aactagcgga gttacttaaa ggattcaata
     2341 tcattattgc aagtgttacg ccattttttc gacgagattt ttttgaaaac aaagatgaac
     2401 tgctattgat ttcccgacat ggtattggtt acaacaatat tgatttaaaa gcagcaaaag
     2461 aacaccagac aatcgtatct atcatacctg ctttaattga acgaaatgct gtagcagaaa
     2521 ataatataac taatttgttg tccatgcttc gacgcacgac acaagcacaa gcaagagtca
     2581 aggacgatcg atgggaagat cgggcggagt tcgtcggtag gacgctgttt aacaaaacag
     2641 ttggtgtgat tggtgcagga aataccggaa gcggagtaat cgaaattttg cgtaacggtt
     2701 ttcgctgtga tgttctagct tatgatcctt acaaatcaga tttatacatc gaaagctttg
     2761 gtgcaaaaaa agtagaactt caagaattac tggaacaatc agatatcatt tgtttatgtg
     2821 cgaatttgac agaagagaac tatcacatga tttctgataa agaagtcgat cagatgaaag
     2881 aaaatgttta tctatctaat agtgcaagag gcgctctagt tgatgaagaa gcgatgatta
     2941 atggtctgaa aagcggaaag atcgcaggat atgccacaga tgttttagaa gaagaaccgg
     3001 gacgtcaaga tcatccgtat ttagcgtttg aacaagtagt gattacgcca catatatcgg
     3061 cctacacaag ggaatgttta gaagcaatgg gtgaaaaatg tgtcagagat gtcgaacaga
     3121 tcagtcagca ccttcttcct gaacgttctg tccagacaaa gagcaactat ataaaaggat
     3181 gatgaagaaa atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt atttatatga
     3241 agaaggtggt atttcaaaag tttctgatct cttgaaatca tacggcgcga aaagagtact
     3301 ggtcacccac ggaacggtat catgggaaaa ggcgctacct aaattagtat ttttgaatga
     3361 cgaaacgatt caattttttt atcatcgtta ttctggagaa tgtagttatg ctgaagccag
     3421 acgaatagct acaatcatca aaaaaatgaa atcgatttcg tagttggcgt tggcggagga
     3481 aaactgaccg atctagtggg ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc
     3541 cctactttag ctagtaattg tgctccatgg acacctcttg ctgtcatgta taaggagaat
     3601 ggacaagcag aagggaaaac agaacacttt tacagacaag ctgctttttt tataacagat
     3661 ccccaattag tgattgattc ccctgttcca tatttcattg ccgggttagc agatacacta
     3721 gcaaaatggt atgaatctga gctcatctta gaacaagatt ttttgcaaga tgaatcattt
     3781 ttgaagctag cacaagatac tgcgaagatc tgcaaagaag agattttgaa tcattctgct
     3841 aaagcaatcc aagatatggc acaaagaaaa ctgacatcgg agttcaaaca gctttcagag
     3901 attatctttg ctgttgctgg tttagttgga ggattaggag ataaatacgc aagaaatgcg
     3961 gcagcacatg ctatgcatga cgctatcagt aaatatattc cggaaagcca tcgttttcta
     4021 catggagaaa aagtagcgta tgggattttc tatcaactgg cactagaagg tcgctgggaa
     4081 aaaatagacg agttatatcc tttgtatcgg gaattagaac tgccggtttc tctagaacaa
     4141 atgggattat ttccgaaaga taaacagata atagataaaa tggctgaatt tattgattcg
     4201 aaagagaaag tgcatttgat tcctatagag gtaacacaga aatcactgat acagtcaatg
     4261 atggaactgg aaggttatat cagaaagatc tgaaggagga ggacacatgg aaacattaag
     4321 tgtagtacaa agtttgttga ttgctttatg ggtagccgcg gttatgtccc gctggttagg
     4381 tggaggagcc acgttgacct tacgtttttc tcctttgatg actggattag tcgtcgggtt
     4441 gatcatgggg gaagttgcac aagcgatgat tgttacagcc gctttgcaaa tgatttatat
     4501 gggggttttt tctccaggag gatcaatgcc agctgaacca tctattgcag cagctatcgc
     4561 agtaccagtc gcattattag gggatctgtc gccagaagca gcgattgccg ttgcggtgcc
     4621 agtcggattg ctcggaagtt acttgtatca gtttcgtttt ttcatcaata cttttttagg
     4681 taaatataca gacaaagctg tagaagattt gaatacaaaa gctatggccc gatcaattat
     4741 tctttatcca acggttgcct catttttatt attcgttccg cttgttttca ttgctttata
     4801 tttaggggcg ccattgatcg ctgatgtaat caaagcttta gagggaacag tcgttatcca
     4861 cattcttgaa gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt
     4921 tatcggtcga aaagattatc tggtcttttt cttccttgct tattttatga gtgttgtttt
     4981 gaaatcttta gatattacga tggttactta tgccattttt gggattatta tcgctttgat
     5041 ctttgtccag tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata acaattcagc
     5101 atcttttgat gacgaagatg actatgatga cggattttaa tttaggagga atgggaaatg
     5161 gctgaaataa tggataatca aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca
     5221 aaagcctatc tgagatggca ttttgcaaac gaaattcctc actcttttga acgttacttg
     5281 gcgccatcac tactatacgg aatgatgcca atcttgaaaa aactttataa agatgaagac
     5341 tctttaaaag cagcatataa acggcagtta ctgttcttca acactcaatt aagctggggt
     5401 ggcggtgtga ttactggcct aatgtcttct atggaacagc aaagagcaga agaagagtat
     5461 gaagaaaaag agatcttgat gcaggatgac ttgatgtata acacgaaagc gggattgatg
     5521 ggggcactag caggaatcgg agatgcaatc gattcaggaa ccgtccaata tatttttatc
     5581 gcaatcgcgg taccttgggc acaacaggga agtcctttag gcgcactttt tccttttatc
     5641 tgctttgggt tgtatcaggt tttactaggg gtatttttcg caagacaagc tttcactatg
     5701 ggccgtaatg ctacaggcct aatgcaaaac actggtgttc agacagcgat tgaaatgcta
     5761 tcagttttag ggttgtttat gatgggggtg cttgcgggaa actatgtaaa agtagaatct
     5821 tcattgaaat ttgcgatatc cggacgtgag tttgttatcc aggaaattct tgatcaaatc
     5881 gtgccaggga tattaccttt agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa
     5941 ggattgaaag tcacacaagc attattatgg ctgactggaa tattaattgt gctagcagga
     6001 atcggtattt tataaaaaga agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt
     6061 gtggatgagc gtttgatcca tgggcaagta atggtaacgt tatctcaaaa aagtggagtg
     6121 aattccattt ttgttgtaga cgaagttgta gcaaaagata aatttatgag agatctttac
     6181 aaaaatgcag gaagccgtac aggacaaaaa acaattgtca ttacaccaga gaaagctaaa
     6241 ttctattggg atgaatatca atttaaagaa tacaattgta ttttgatcgc aaaaacagtc
     6301 caaacgattt acgacttagt aaaacacgga atccccatgg aagaattgaa tattggaggg
     6361 attgctcaaa aaaatcctga tacagatctt ttagtgacga aatctgttta tttaaataaa
     6421 tcagatgctg aaaaattgaa agaaatgaat gaacaatatg gcgtaaagaa tatttatttt
     6481 caagccactc catctgcacc taaaacagga ttaaaagatg tattgaacaa gtttgatttg
     6541 tcataaacaa atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg
     6601 tttcgtgatt gggaatttcg ttatcgctat atttaccgta agcgccgtac aaaaaagagt
     6661 aaacagcgtt ttctttcagc attagtatca gatatttaca gtatgagaac agatgtgaca
     6721 gtcattgctt acgatacgct agcctaccga tcaaaaaata tttatgttgg agatatcgaa
     6781 aaagcagaga aagttatctg tacttattac gatacacccg ttcatgcatt aggaagttat
     6841 ttcatgtttg attggaaaga ccaaaggaaa aaaacaatct actctatatt attgtccttc
     6901 attttgttgt tcagtttagg ttggtgggga atgatgatat ataacaaaaa tcctcatcac
     6961 gtattcgatc tgctatccgt ataaacaggt atcactgtac tagcatttgg gagctacttt
     7021 ttcttattgg gaaaagcagc tcgcggatgg cctagcagac aaacttttat tcgaaatact
     7081 tcatccatat taacgatgct agaaatgatc cgtacgattg atgatccgag tgtagcgtat
     7141 gcgtttgttg atgaaggctg ttatggagaa aagggcttag acagcgtgag atcgggtatg
     7201 aaaaaagagg ctattctttt ttatctagac tctgttgggg cagatacacc gctacagttt
     7261 tcgggaaatt atttttcaaa taaagaacag tggctgaaac aagttgataa gttgaaagaa
     7321 aaaaacgtca attatatttt tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc
     7381 aaaacagatt tacgagggaa aacatttaat tggcaaaatg ccaatcagat tattgctctt
     7441 tttcgataag taaaaagtgc aaaacaagga gtgagaagac taatgttgga aatcatgatc
     7501 gttacacatg gtaagttgag cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca
     7561 tcgagccata ttgaaacaat gtgcttagag tctggggagg acatccaaac attcggagag
     7621 aaggtcaaag aaaaattaac tatgatcaac acagcagaac ccatgctagt atttgttgat
     7681 ctaattagcg caagtccgta caatcaagcg ctcttggcag tcaatgaact gactgaagaa
     7741 aagaaagcag ccacttatgt catagggggc gtgaatctgc caatgttgtt agaagcaatc
     7801 aatcatcaga tgcttcacac accgatagaa gaagcggtgg aacaaattgt caaacaagga
     7861 aaagataatt taactgtttg gcatgcttca gcggaagaaa aaaaattaga tgaggacgat
     7921 ttttagaagg aaggctgaaa agatgaagtg gggatttaga tggtatggtg aaaaagggga
     7981 ttcgatccca ttaaaatatg ttcgccaaat accgggaata aatgggataa ttggaacttt
     8041 gctgactaaa atgcctggag atgtttggga gatttcagaa attgaggagt tgaaagcatc
     8101 tgttgagagc caaggactgg aactgcttgg aattgaaagt gtagcgatcc acgatgcaat
     8161 caaggcaggg acagatgaac gagattacta tattgataac tatatacgaa caatcaaaaa
     8221 cttgtctcga tgtggggttc gattgatttg ttatagcttc aaaccaattt ttggatgggc
     8281 gaaaactgat ttgtttaatg aaaacaacga tggaagctat tctcttttat acgatcagaa
     8341 aatcgtcgat cagatggagc caggagaaat gtatcaattg atacacagcc agtcaaaggg
     8401 attcaaattg ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact
     8461 gtatgaagga atgaccgaag aagaattatt tgaaaatctg acttattttt tagaaaaagt
     8521 gattccagtg tgcgaaaaag cagatgtaaa aatggctatc catccagatg atccgccatg
     8581 ggaacttttt ggttttccta gagtgacgaa aaatcttggc gacttgaaga aaattctaaa
     8641 tgcagtagat tctccatata atggactaac gctgtgcaca ggatctttag gggcaaatcc
     8701 ggaaaacgat ttagttgaaa tcatccatga ggtaggttcg cgcatccatt ttgtccactt
     8761 cagaaacgtg gagtttttag gagaaagaaa gttcaaagaa acggcacatc cctctacaga
     8821 aggctccctt gatatgtatc atattatgaa agcgttggtg caagaagggt tcgacggtcc
     8881 tatacgccct gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct
     8941 gtatgatcgt gcaatgggac tcacttacat gcaaggtcta tatgaggcga taagtaagga
     9001 aaaaccactg ggagagcatt atgaataaaa tcaagctctt catctttgat atggatggcc
     9061 ttttattaga aactggacgt ttagcttatc gtgcttatgt aaaatcagca caaaagtatg
     9121 attatgaaat gcgtaaggaa gtctattatt tactaactgg ccaaacagaa atggcgataa
     9181 gagaacaaat gggcattttg tacggagaag atgttccata tatcaaatgg cgagaagcaa
     9241 tcaatcaata taaagaaaaa atcgtgaaag aagataagag agtatatacg aaaaaaggag
     9301 cagaagaaat tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta
     9361 atacacgggt aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc
     9421 aaatcatttc aggggatgat gtaaaaaaag gaaagccgga accagagatc tttttgaaag
     9481 cttgttcaaa aatgaatatt cctccaagtg aagcacttgt atttgaagac tcgattgcag
     9541 ggatcgaagc agcaaggcga gcgggcattt tgtcttttct catcaaagat catttgatag
     9601 ggttgcccga tcataatggg aagcataagt tgaaagtaga tgtaagttta tatgaatcca
     9661 ataaagcaga ctatgaattt tatgacctgc atcaagcaaa acgattttta gaaagtaaag
     9721 ggttgtatct ctaataaaga ggactatttt ctagtcctct ttatttattg tcctgcacga
     9781 ctgagggaac agatgaaaaa gaagtaattt tttattttga agtatctacc ttatttgtag
     9841 tgaacaattt agaacaaaca caaaacgcat ctactgttat ctaccagtag atgcgtttaa
     9901 agagaagaac gagaatcgtc catatgaggg tttccactcc ttatgcagat ttttttaatt
     9961 cttgcactgc ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa
    10021 aaacgacaaa tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat
    10081 agaatgtcat agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga
    10141 taacgaaatt ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt
    10201 taaagtcaaa agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc
    10261 ggttattcaa ctttttcttc gattcgaaga caatagaaga caatgaattt atatttcatt
    10321 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    10381 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    10441 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    10501 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    10561 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    10621 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    10681 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa agacggtttt tttgtacgaa
    10741 aaaatt
//
LOCUS       DAYYAG010000093        10551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000093 DAYYAG010000000
VERSION     DAYYAG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.095"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..1041
                     /locus_tag="ACHARQ_002105"
     CDS             145..1041
                     /locus_tag="ACHARQ_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8410505.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1191..2132)
                     /locus_tag="ACHARQ_002106"
     CDS             complement(1191..2132)
                     /locus_tag="ACHARQ_002106"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8410506.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2258..3379
                     /locus_tag="ACHARQ_002107"
     CDS             2258..3379
                     /locus_tag="ACHARQ_002107"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8410507.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3637..5139
                     /gene="eat(A)"
                     /locus_tag="ACHARQ_002108"
     CDS             3637..5139
                     /gene="eat(A)"
                     /locus_tag="ACHARQ_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8410508.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5206..5733
                     /locus_tag="ACHARQ_002109"
     CDS             5206..5733
                     /locus_tag="ACHARQ_002109"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8410509.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5944..7005
                     /locus_tag="ACHARQ_002110"
     CDS             5944..7005
                     /locus_tag="ACHARQ_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8410510.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7008..7904
                     /gene="murQ"
                     /locus_tag="ACHARQ_002111"
     CDS             7008..7904
                     /gene="murQ"
                     /locus_tag="ACHARQ_002111"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8410511.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7918..9900
                     /locus_tag="ACHARQ_002112"
     CDS             7918..9900
                     /locus_tag="ACHARQ_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8410512.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9965..10411)
                     /gene="nrdI"
                     /locus_tag="ACHARQ_002113"
     CDS             complement(9965..10411)
                     /gene="nrdI"
                     /locus_tag="ACHARQ_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8410513.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3444 a         1856 c         2279 g         2972 t
ORIGIN      
        1 attttaatag aagaatgatt tatttcatca tattttcata caaatgttaa attttcatac
       61 aaatatagga aacgttttac tggcttttta ctatttaaac aggctatcat aggaaaaagt
      121 gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg tactgctagt tgcaaaaaat
      181 aaatcacttt attttttgcc ttccatccgc tctttagatg tttggtccgg aaaagattta
      241 ctacagtcgg aattcattaa atcagctaga caacagttgg caaattatca gctgatcgtt
      301 tttttagatt atggatttga tatggagatg gctcgacgcg tgcgcccatt taccaatgca
      361 aaaattgtgt tatttttttg gaatcacttc aaagcagaac acgaggcgat gctgaagcag
      421 gcacaagatg aaccggcaat cgatgagatt tatcattttg attttttgga agcaaaagaa
      481 cttgggctga aacataatag ttcattttat tcgaaacata tgaatttttc ttctagcgaa
      541 ccaaagacgg atctattttt tggtgcaacg aataatggga gaaaagaacg agcagaaagc
      601 tataagcagg aatttataaa aagagatgtt acgaccaact atttcattct tccacatcga
      661 gggaatgaac aacctggata tcttacttat gaagagtacc tgaaaaaaac aggggaatcg
      721 cgcgggattc tggaactgtt gcgggagggt cagcaaggag tgactttgcg gacatttgaa
      781 tccatgtatt ttcaaaagaa actagtgacg gataatcaag cgattgctca ttatcatttt
      841 tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt tagatgaatt accggaattt
      901 ctgcatacac catttaagcc aatcctatca gagctattgg aattttatga tgcagaaaat
      961 tgggcaaaac gatttatcac taacgattcc actatttatg agcaatacga atatgacctc
     1021 tcctctgttg aaaacaaata aaaaacctac attttgaaga gattatgtac tgaaacaaac
     1081 aagcacaatt cttatggcat catgagagtt gtgttttctt tttgatcttt tattgggctt
     1141 aaaaaaagag aaccagctgc tgattcagct gattctcatc agagagcctt ttattctgcg
     1201 ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata tggtgtagca
     1261 atatcatatt tttgtccttt tcgccaaatg gcaccattga tgtagtcgat ttcagtcaaa
     1321 cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat gttgtcagga
     1381 tcataagtag aagcaatatg ttggtacact tcttcttgat ctagtatgat tccttctttt
     1441 gctgcgatag aagcaaattc agatacgatt gttcgaacca tgctctctgc cgctttttgc
     1501 gcgcctagtt cagcaatatt acaatccaaa atcgtgcata aaccattcaa tgttccattt
     1561 acacaagctt ttcgccaaat ggaataacga acgttatggc tatagatcgg atttaaattt
     1621 gcttcttgaa agacttctac aacttttttt gtaaatgctt cgccttctgg ttccaagttc
     1681 tctagttcga tttctccatc acctaacaat gtgacttttc cgggaccagc tagaccagct
     1741 gtccacatcg tgatcccaaa taagatattt ttctttggca catatttttc cagtacatct
     1801 tcatgaccta atccatttag taaacacaaa acataagtat tgtcagtaat aatggattgg
     1861 atcgaacaaa acatatcatc taattgattg gcttttgtta atgctacgat caagtccacg
     1921 tgttcgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc gaccacttct
     1981 ttaccgttaa agtcagcaat caacccattt gtacgaatgg cttcaatgtg agcaggccaa
     2041 cgatcgatca agaggacctc atttccgctt tgatgtagca tcaatccgat tcggctgccc
     2101 attgctccgg ccccagctat tgcgattttc atgttttttc tctcctttat tcaatgagtt
     2161 cccttttagt atatagaaaa atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa
     2221 aaaagtgtct tacttaagaa gaaagtgagg gatacagatg gatattttgg cggcaattga
     2281 ttcatttaaa ggaagtgctt cttcatttga actaaatcag gcagttctaa atggctggga
     2341 agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga
     2401 agcaatctat tacgcgctca ggggaacatg gcgtaccatt gatgggatgg atctcttatt
     2461 ccgtcagcga aaaatcagct atttattgac aacttggcaa ggaaaaaaca ccgcagtcat
     2521 cgaatctgca caagcattgg ggttagatct acttgataaa ccaacggatc aaactatccg
     2581 acttgcttcc agttatgcct taggagaact tattaaagat gcactgcttc aagaagtaga
     2641 acaaattatt gtcactttag gaggaagcgg gtgtagcgat ggtggtctag gattactcca
     2701 atccttgggt gcttctttag aagggtacac agaaggaaat cctttattat ctactaaaaa
     2761 acttcatttt gatcgaagaa acgttcttgt ttctaaagta aaattgatta tcgctgcaga
     2821 tgttaccaat ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg
     2881 aggaatagat acgacgctag ctttcttaga taaacaagca ggaaagattg ctcagcaaat
     2941 aaaagatttc tgggaggtgg atttagatga tattccagga agtggagcag caggcggtat
     3001 tggtggagcc ctttttttgt tgggagcgaa gatggtttct ggtttcgatt tagtcagcca
     3061 aatcactgga ttggaaaaaa agatcgaaca agcagatgtc atcattacag gagaagggcg
     3121 gatcgatcag caaacgatcc acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa
     3181 atatcgaaaa ccaataatcg ctatttgcgg aagtagagac caagagctag aaggactttc
     3241 tgattttctc ccaattgtct taagtattca tttgggtcca ataggtttag aagaagcaat
     3301 ggaacacagg caaacattag aaaaaacaaa aatcttagga cagacactta gcagattttt
     3361 caccatcctc ccgaagtagt tgacaaaatc attttcttta tgctatcgta atttcaaata
     3421 taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc
     3481 agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt
     3541 ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga
     3601 aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat
     3661 ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt
     3721 gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg
     3781 aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc
     3841 tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt
     3901 acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa
     3961 atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca
     4021 cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat
     4081 atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg
     4141 gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa
     4201 agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt
     4261 gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa
     4321 acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa
     4381 gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct
     4441 gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag
     4501 aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg
     4561 tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta
     4621 ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac
     4681 ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg caaggttata
     4741 ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac
     4801 aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg
     4861 aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg
     4921 agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta
     4981 tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa
     5041 ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat
     5101 aaagtatcta ctgagattat ttctcttgag agaatctaac ccttgtaatc tcaagaacaa
     5161 caaggtatgg ttaaggggaa ctttgccaac gaaggagaga atcacatgct ttatgtaacg
     5221 agacacggag aaacaacttg gaacgcacaa ggattagttt gcggacgggc agatgtgccg
     5281 ctgactgaaa aaggacagat gcaggcacag aaactagcag aaaaggtagt agatttgcct
     5341 gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca
     5401 gttgctgatc gtctcagctt gccgctgacc gttgatgaac gattggtaga aatggatttt
     5461 ggggattatg atggactgcc aagcaaagat gagaactttc aaaaagcacg tttagcgttc
     5521 gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat cgttccttta
     5581 ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca taatgccttg
     5641 ataagagtga tcaatgctta ttttcatccg atgcctaatg aggggttctt tacattcatg
     5701 gtagataata cagaattggt ttcttatgag taattatgtt tttgtgttat ttaaataaaa
     5761 tatttcaagg cactgctttt gcttagcagt gcctttttta ggttatgaca attcttttga
     5821 taatataaac tcaaccttta gaaatgaaat ttaattattt taataaaagt attgaaattt
     5881 tgtttcttta gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat
     5941 aatatgtttg gcttttcaat atttatgaac gattctttgt cagaagagaa gaaacagtat
     6001 atccgccaaa tggcatccaa tggctttgtc ggtattttca cttccatgca tataccagaa
     6061 gatgatgttt cggcatatca aaagcgattg atcgaactag gaagcgaagc gaaaaagaat
     6121 aaactagaat tgatggtcga tatttcagga gaggcattgg aacgagcggg tttttcatta
     6181 gaagatctaa cgcctttgaa agcaattggt gtaacaggtt taagaatgga ttatcatatt
     6241 tccaatcagc agattgccga ctggtcacat caattgaaaa tcagtttgaa tgctagcacg
     6301 atcacgccta aagatatcga tgaattgaaa gaagcagaag ctgatttctc acagattgaa
     6361 gcttggcata attattatcc acgtcccgaa acagggctgg acaaagaatg gtatcagaaa
     6421 aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc aggagataca
     6481 gagttgagag gcccactata tcaaggattg ccaactttgg aagaacatcg agggatccat
     6541 cctcttgcag gcgctctaga tctattagca agtaacacgg atattgttta catcggagat
     6601 gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc
     6661 ttgctccata cggaacctgt agatgaagaa ttttacgaat atatattagg gaagcatacg
     6721 aatcggcaag acgatgcaag agatgtcata cgaagcgtag atgcacgttt tcgagagatt
     6781 ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag ggtctattac tttagataac
     6841 gaaaagtatt tgcgctatat gggtgagatc caattaacca aatatgattt gccagcggat
     6901 gaaaaagtga atgtagtagc taaagttatc aaagaagagc taccattgat caaccaaatc
     6961 aaagctggta tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca
     7021 atttagagaa cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgagatga
     7081 gtatttctca agccgttcaa ctaatgaaca aagaagatca gaaagtggcc gctgcggttg
     7141 aaaaagaact gacaagtatc gaaccagtga ttgctaacac gattgagtcc tttaaaaaag
     7201 gtggacgtct gatttatatg ggagccggaa caagtggaag actaggtgtg ttagatgctg
     7261 ctgaatgcgt accgacattc ggtgtagaac cggaaatggt cataggtcta attgctggtg
     7321 gagaagaagc aatgactgta gctgtcgaag gggccgaaga ttcttatgag ctagggcaag
     7381 aagatctaat caagctgaat ttgacaaaaa atgatatagt tattggtatc gcagctagcg
     7441 gtcgtacacc atatgtgatc ggtggattag attacgcaaa atcgatcggt gctgtcacag
     7501 gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc ccaatcgaag
     7561 tcgatgcagg tccagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa
     7621 aacttatctt gaatatgatt tctactatca gtatgattgg tataggtaaa gtattcaata
     7681 acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca aaacgaatca
     7741 tcatgcaagc gacagaagct gattacgaaa cagctgaaaa gtactttgaa gaagcagatc
     7801 aaaatgttaa gctggctatc gtcatgattt tgacaaatag tgataaagag gaagctcaag
     7861 caaaattagc tgaagcaaac ggatttgtaa aagggacctt atgaggggga aaagaccatg
     7921 gcaaaaaacg aactgacttt ggaagaactg gcaaaacaaa tttataaagg ggcaggcggc
     7981 atgggcaatg tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa gatcatcaat
     8041 gatgatcttg taaatgtctc tgaattaaaa gcgattccag gagtgcttgg tgtagtagaa
     8101 gatgaacagc ttcaagtcat catcggacct gggaaagtga acaaagtagc acagcaaatg
     8161 gttgatttgg caggcgtgaa attaggagaa aaactttctt ctgtaagtac agctacagca
     8221 aattcatcag catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca
     8281 caaaaatcga aacaaaaacc atctaaaatc aaatcggtat taaaagacat ttctaatatc
     8341 tttgtgccaa tgattcctgc atttgtcggt actgggatcg tggcagggat tgccgcagtt
     8401 ttagcaaact tagtcacagc cggaacactt gatgcagcaa catggcaaca atatatcgat
     8461 gtgatgaaca tcttgaaaaa cgggatgttt agctacctag ttatctatac aggtatcaat
     8521 gctgcacaag tttttggtgc aacaccaact cttggtggag ttatcggggc cgttgtgatg
     8581 ctgacaggga tgaatccgga agcaccactg aaaaacttat ttactggtga agctcttgca
     8641 gctggacaag gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc aatcgttgag
     8701 aaaaaactgc ataaagtagt acctgatgcg gtagatatca tcgttacacc aactctttca
     8761 ttgattgcca tcggtctgat tgaaattttc ttcatcatgc ctttagcagg tttcatttct
     8821 gatggcatgg tcggtgggat caactgggta cttggcgttg gtggagcatt tgccggcttc
     8881 gttctaggca cattattctt gccgatggtt atgttcggac tgcatcaaat tttgactcca
     8941 atccatgtgc aaatgattga tgaaactggt cgtacgctat tgcttccaat tcttgcgatg
     9001 gctggtgcag gacaagtggg tgcagcattg gcattatgga ttcgctgcaa aaaagataaa
     9061 gaattaacag aaatgatcaa aggagcttta ccagtcggga tcctaggaat cggcgaacca
     9121 ttgatttacg gagtaacatt acctctagga cgcccattta ttactgcttg tatcggtgga
     9181 ggtatcggcg gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc tattggacct
     9241 tcaggagccg ctttgatccc attgatctct gacggtaaat ggttaggcta catccttgga
     9301 ttattggcag cttatgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac
     9361 cgtttagaaa aagaagaatt agcgactgaa acagccacag taaatgaagt gcctgaaaaa
     9421 gtagtacctc aaacttcagc tgaaagtgtg actatcctct catcagttgc tgacggaaca
     9481 gttgtttctt tagataaagc aagtgatcca gtttttgcac aaaagatgat gggtgaaggc
     9541 tactttgtag aaccagaaaa cggacatatc tattctccag ttactggggt agtcagctct
     9601 gtcttcccaa cgaaacacgc tattgggatc acaacgccaa atggtttaga aatcttgctt
     9661 catatgggaa tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa agtaacagaa
     9721 gggcagcaag tgacacctga tacgcttgta gcagaagctg acttagaagc gatcaaagca
     9781 gcaggcaaag aaacatccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt
     9841 gtcttggaaa aaacaggtaa agcaaaagca aaagcacctg tgatggatgt caaagcttaa
     9901 tatgatagaa acaatagaaa cgaaagaaga aactgaaatc tatttttcag cttcttcttt
     9961 tttattattt attgttttct gctaaaatct gataaattcg tgtcacatca gatggtgttc
    10021 cccgtaattc ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat tgtttggctg
    10081 ttagacaata ctgattattg aagtttttat ttccgctacc gactacacca agacaaagtt
    10141 tatgattgtc ttcatgttcc agatattcac gcatcgtttc ggtcaagatt tctgtatcac
    10201 cgttatctaa accatttccg ccgtctaaat aagtaggaac gaaagtaaaa aatggttctg
    10261 tttctttcgt aaaatcacta ttttcatgga tttcttttaa agtaacttca ggattgacag
    10321 gatcttctgc atgcattttt tcagcatatt ctgctagatg tttggcaaag gcacgcgtat
    10381 ttcctgaaat cgatatatat aaaatgttca tggatcatcc tccaattctt tccttttagt
    10441 atagcatctt ttttaagttt tttggtggtg taatttggaa agggactagg cgctttgctt
    10501 gtataatgag aaaaaagccg gtagaattat atttctatta aagtttttat t
//
LOCUS       DAYYAG010000094        10482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000094 DAYYAG010000000
VERSION     DAYYAG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.096"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACHARQ_002114"
     CDS             34..657
                     /locus_tag="ACHARQ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410514.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACHARQ_002115"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACHARQ_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8410515.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACHARQ_002116"
     CDS             2153..3034
                     /locus_tag="ACHARQ_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8410516.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACHARQ_002117"
     CDS             3037..3807
                     /locus_tag="ACHARQ_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410517.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACHARQ_002118"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACHARQ_002118"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8410518.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACHARQ_002119"
     CDS             5118..6281
                     /locus_tag="ACHARQ_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8410519.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACHARQ_002120"
     CDS             6398..6808
                     /locus_tag="ACHARQ_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8410520.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACHARQ_002121"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACHARQ_002121"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8410521.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACHARQ_002122"
     CDS             9381..9665
                     /locus_tag="ACHARQ_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410522.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACHARQ_002123"
     CDS             9825..10061
                     /locus_tag="ACHARQ_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8410523.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACHARQ_002124"
     CDS             10058..10456
                     /locus_tag="ACHARQ_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8410524.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3655 a         1762 c         2221 g         2844 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaataat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca at
//
LOCUS       DAYYAG010000095        10463 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000095 DAYYAG010000000
VERSION     DAYYAG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.097"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..230
                     /locus_tag="ACHARQ_002125"
     CDS             6..230
                     /locus_tag="ACHARQ_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8410525.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(435..755)
                     /locus_tag="ACHARQ_002126"
     CDS             complement(435..755)
                     /locus_tag="ACHARQ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8410526.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(903..3137)
                     /locus_tag="ACHARQ_002127"
     CDS             complement(903..3137)
                     /locus_tag="ACHARQ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8410527.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3616..3882
                     /locus_tag="ACHARQ_002128"
     CDS             3616..3882
                     /locus_tag="ACHARQ_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8410528.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3882..5609
                     /gene="ptsP"
                     /locus_tag="ACHARQ_002129"
     CDS             3882..5609
                     /gene="ptsP"
                     /locus_tag="ACHARQ_002129"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8410529.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5773..6819
                     /locus_tag="ACHARQ_002130"
     CDS             5773..6819
                     /locus_tag="ACHARQ_002130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8410530.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6836..8059
                     /locus_tag="ACHARQ_002131"
     CDS             6836..8059
                     /locus_tag="ACHARQ_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8410531.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            8148..9035
                     /locus_tag="ACHARQ_002132"
     CDS             8148..9035
                     /locus_tag="ACHARQ_002132"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8410532.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            9121..9351
                     /locus_tag="ACHARQ_002133"
     CDS             9121..9351
                     /locus_tag="ACHARQ_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8410533.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9408..10373)
                     /locus_tag="ACHARQ_002134"
     CDS             complement(9408..10373)
                     /locus_tag="ACHARQ_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8410534.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3382 a         1834 c         2118 g         3129 t
ORIGIN      
        1 gacacatgga aacagaatgg caaagacgtg aaagaaaaaa acagaaaaaa gaagagatgt
       61 tacgattatg tcgattatta gatgtaaaag actgggacaa aaacgagcaa attgcacaaa
      121 aagtacgatc aatcatccat aaagaggaaa aagttaaaaa taaagacacg cgtagaactg
      181 aaaatgaaaa gagaaaagta caaaagaaaa gagaaaaatc aaaaagctag aaagaaaaag
      241 aggctgagac agaagcgttt aactccaaga aataagctgg aattcacaaa aatttgaaaa
      301 gcaattttcg tgaatttcag cttatttccg aaggagttgc ttctgctttc gccgtttata
      361 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct ttaattatta
      421 tgttcaaaat ttgtttacac ggctaatgta gcgcctaatt catgttttgg aatcgagatt
      481 tcaacaaggc ctgccaattc gattactttg atttcatcct cgtggtaaac ggcgtctttg
      541 attttatcta agaaataatt tttagcatct tcgatttctt tcggctggat tttacgtttg
      601 tcattgatca gcagaatttc attatgttca ggggcttcag tgtagacaat agtggtattt
      661 ccggctgtgt agaccttgac tagatgggca tcagtgtttt caagctggtt taatacaaga
      721 cgggaatggc tgtttgttac atttaccaat ttcatggcgc tcacctcttt tatgtatttt
      781 taattgcttg tttctcttat tataatttct tttttataaa atgaacagta ttttgacttt
      841 tctgaaaaaa gaatgagacg gcatttctca aagaaatacc gtctcacgct ataaaactgg
      901 atttattcag ccgtttcttc ggctgattct ttagccaaac gttctggttt agaagtaacg
      961 atgatttttc catctttatc cagttttgct ttcaactcat ggatagaagg gtgatcaaga
     1021 taaaattcag cgataccatc ttcgatttgt tcttggatcg tgcgtcgaag aggacgagca
     1081 cccatagatg gatcatagcc taagtcaact aatttttctt tgacattcgt aggaacttcg
     1141 atgtgaagtt gctgtgctgc cagcatttgg ttgacatcgt ctagcattag tgtgacgatt
     1201 gtcatcaagt tttcttttga taatgcactg aattcgatga tcccgtcaaa acggttcaga
     1261 aattctggtg taaagtagtt gtttaattgg ttcaatacag aacgagtaac accttcgcgt
     1321 gccgcaccaa aaccaacgtt tgcttctact ttaccagttc cggcattact tgtcataatg
     1381 atgatcgtat ctttaaagct gaccgttctt ccttgtgcat ctgtcaatcg accatcatct
     1441 aagatttgca agaacatatg caaaacatct ggatgagctt tttctacttc atctaacaag
     1501 acaagactgt aaggatttct ccgtactttt tctgttaatt gaccagcttc ttcgtatccg
     1561 acataacctg gaggagaacc aatcaattta gagacactat gtttttccat atattcagac
     1621 atatcgaaac gaatcattga atcttgtgaa ccgaatagtt cgtaggcaag ttgtttcgca
     1681 agctctgttt tacctacacc agttggtcca acaaataaga aggagccgat tggacgattc
     1741 tgtttgttta atccaacacg gtttcggcga atcgctttcg ctactcgatc tacagcgtta
     1801 tcttgtccga tgacatgagc tttcaagtca tctgctaagt ttttcagttg tgtctgttct
     1861 ttttctttta attcacccac tggaatacct gtccgttgtt caacgatttt ttccatatct
     1921 ttttcggtaa tcacaggtgt ttcctcttca gtgagctgac gttctttcat tttttgaagt
     1981 ttattgatct gatcgcgata ataagctgct ttttcgaaat cttcttctcg agaagctaaa
     2041 actttttgtt cttctgcttc ttgcagtttt ttctcgattg ttttaggatc aacgatttgg
     2101 atcgtcaagt ttttcttaga acctgtttca tctagcaaat cgatggcttt atccggcaag
     2161 aagcgatctt ggacgtaacg attggataaa gttgcggctg cttcgatggc ctcatcagta
     2221 tattttacat gatgatagtc ttcataacgt ttttgcaaac ctttcaaaat actgatcgtt
     2281 tcttcgacag ttggttcatc tacccgtaca ggttgcatac gacgttctaa agcagcgtct
     2341 ttttcgatga tgcggtactc atttaaggta gtagcaccaa ccatttgcaa ttctcctcga
     2401 gctaaagctg gttttaaaat attaccagca tccatatttc catcaccagc agaaccagca
     2461 ccgacgattt cgtgaacttc atctatgaat aggatcacat tttctgcttg acggatctct
     2521 tcaatcaatt tttgcatacg ttcttcaaat tgtcctcgta taccagtacc ttggactaaa
     2581 gatacgacat ctaaacgaat gacttctttg tccattaatt tttgcggtac atcaccatct
     2641 acgatttttt gtgctaaacc ttcaacaact gctgttttac caacacctgg ttctccaatc
     2701 aagacaggat tgttcttggt tcggcgattt aaaatttcaa tgactcgttt gatttcgtca
     2761 tcgcgtccga tcacaggatc gatatcacct tgtcgtgctt gttctgtgat attgattccg
     2821 tattctccca ataaaccttc tgtattgcct tgaggtggct gaccaccatt gaagttgttg
     2881 tttccgccaa attgtgttgg tggtgtttgc tcataatttc cttgctgttg catttggcga
     2941 gacatggaac ggaaaagatc gtccaaactg ccaaatccaa atggatcatt ttgggccata
     3001 ttagtcagac ctccattttg ttgatttttg attttttgat aacagctctg acagtaatcg
     3061 agttgtgtcc gctgaccatt gacagtggca tacaagtgaa tcgttgcctc attcttgccg
     3121 cagttttgac aaagcatgaa tcattcacgt cctttcacaa aaatgggtac atccatattt
     3181 tacatctcgt ttttcatgag gtaaaagaat aacccctagt tttttaagag atttatgagg
     3241 tagaaaaggt gaaatctcta ttgaccattc ctgacctttg actcttatta tactaaaaat
     3301 aaaaagggaa gcaagtaatt cgtttcatat gaaaacaaag agggtcaaag gaaagaatga
     3361 taaacgtcag gagaagagaa agcgacaaga aaaaggaaat aaacacgttt gttataaaat
     3421 gtgaagaaaa ggatgaaaaa gaatccggaa taatttgaat acttttatct gaaattgaat
     3481 gaaaactgtt gtatctaacg aaaaaaaatg ttaatcttta cagatgaaag ggtggcattt
     3541 gttaggattc tctgaattac taaaaaagaa aaccctcaca taaaaacaac actcacttaa
     3601 aggagaccga ttaatatgga aaagaaagaa tttcacgtag tagcagaaac tgggatccac
     3661 gctcgtccag ctacactatt agtacaaaca gcaagcaaat ttaactctga tgtaaactta
     3721 gagtataaag gtaaatcagt aaacttgaaa tcaatcatgg gcgttatgtc tttaggtgta
     3781 ggtcaaggtt ctgatgttac tattactgca gaaggtgctg acgaagctga tgcaatggca
     3841 gcaatcgttg aaacaatgaa gaaagaaggc ttatctgaat aatggttgaa atgctaaaag
     3901 ggattgccgc tagtgacggt gtagccgttg caaaagctta cctgctagtt caaccggatt
     3961 taacattcag taaggcaaca gtagaagata ctgcggctga agaagctcgt ttggatgctg
     4021 cattagctaa atctactgaa gaattgcagc aaattcgtga aaaagcagcg caaagcttag
     4081 gtgaagcaga agcgcaagta tttgatgccc atttgatggt tctttcagat ccagaaatgg
     4141 taggtcaaat caagcaaaat attaaagaca acagcgtaaa tgctgaatca gcgctaaaag
     4201 aagtaacgga tatgtatatc ggtatgttcg aagcaatgga agataatgct tatatgcaag
     4261 agcgtgctgc agatattcgt gacgttgcta aacgtatttt agcgcactta ttaggcgtta
     4321 ctttaccaaa tccatcaatg atcaacgaag aagtggtcgt tgttgcacat gacttgactc
     4381 caagtgatac agcacaatta gatcgcaatt ttgtaaaagc gtttgtaaca gacattggtg
     4441 gacgtacttc acattctgct atcatggcac gttctttaga aattccagca atcgttggga
     4501 caaaagaaat tactgctaaa gtcaaagaag gcgatatttt ggctgtcaac gggatcgaag
     4561 gcgatgtaat catcgatcct acagatgaac aaaaagctga atttgaaaaa gcaggtgcag
     4621 attacgcagc acaaaaagca gaatgggaaa aattaaaaaa tgcagaaact gtcacagctg
     4681 acggcaagca ttttgaatta gctgcaaaca tcggaacacc aaaagatcta gttggtgtcc
     4741 ataataatgg tggagaagca gttggtctgt accgtactga attcctgtat atggattctc
     4801 cagacttccc aacagaagat gaccaatatg aagcttacaa agctgtatta gaaggtatgg
     4861 aaggaaaacc tgtagttgtt cgtacaatgg acatcggtgg agataaagaa ctaccatatc
     4921 tgcaattacc tcatgaaatg aatccattct taggatatcg tgcattgcgt atcagtttat
     4981 ctgaacaagg cgacgaaatg ttccgtacac aaatgcgtgc attgcttcgt gcatctgttc
     5041 atggtaattt acgtatcatg ttccctatgg tagctacact caaagaattc cgcgcagcaa
     5101 aagcgatctt tgaagaagaa aaacaaaaat tgataagtga aggcaaggaa gtatctgata
     5161 cgatccaagt cggcatcatg atcgagattc cagcagcagc agtcttagca gacaagtttg
     5221 caaaagaagt agatttcttc tctgtaggta cgaacgattt gattcaatac acaatggcag
     5281 cagaccgtat gaacgaacgc gtttcttact tgtaccaacc atataaccca tcaattttac
     5341 gtttgatcaa aaatgtaatt gatgcagcac acgctgaagg aaaatgggca ggtatgtgtg
     5401 gagaaatggc tggagatcaa acagctgttc cattgctagt aggtatgggt ctagatgagt
     5461 tctcaatgag tgcgacatct attcttaaaa cgcgtagttt aatgaaacgc ttggacacag
     5521 cgaaaatggc tgaacttgct gatcgtgcat tgaaagaatg cgatatgatg gaagaagtcg
     5581 ttgaacttgt tcatgaatat gtaaaataaa caacgtatca aaagctggtg tggcttgtaa
     5641 aagagctacg ccagtttttt tgttcttttt taagaaaacg tttttgtcag taagaataga
     5701 caaaaatagg gcttaagttt gagtgacagt ttgtaggcta tgttatacta tataagtgaa
     5761 gggggacgta aggtgaaggt attattatat tttgaaagtg aaaagatgct tgcaaaatct
     5821 ggaatcgggc gtgcgcttga tcatcaaaaa cgtgctctga cggaagtcgg tatctcttat
     5881 acactagatg aaaaagaaga ttatgacatc ctgcacatca atacatacgg aatcaatagt
     5941 cataatatgg tcaataaagc cagaagagaa ggaaaaaaag ttgtctatca tgcgcattct
     6001 acagaggaag atttccgaaa ttcctttatc agatcgaacc agctttctcc tatcgtaaaa
     6061 aaatatctgg taggattgta ccaaaaagcg gactatctga tcacgccaac accttattca
     6121 aaacagttgt tggaaagcta tggtatccgt gtgccgattc aagctatttc caatgggatc
     6181 gacttagaga aatatcaccc tgatccaatg aaagaacaaa agttcagaga atatttcaaa
     6241 ctgtctccgg atcaaaaagt aatcatttgt gtcggtttgt ttttcgaacg taaaggaatc
     6301 attgatttcg tagaaattgc taagaagatg cccgaataca catttatttg gtttggtcat
     6361 gttcccatgt actcgattcc tcggaacatc cggaaaattg taaaagaaga tcatcctgaa
     6421 aatgtcctgt ttcctggtta cattcgtgga gagatcatag aaggtgcgta ttcaggtgca
     6481 gatctattct ttttcccttc ttatgaagag acagaaggga tcgttgtttt agaagcttta
     6541 gcaagcaaac aaaacgtact tgttcgagat attccagtgt ataatggctg gttagaagat
     6601 agcaagaact gctatatggg gaagacaaac gaagaattta tccgactgat ccaacagatt
     6661 accaatcaag aactaccgag tacgacacaa gccggttatc aaacagcaaa agagcgaagt
     6721 atcaataaaa tcggtgaaga acttaaaaat gtatacgaaa gtgtactgaa tgaaaaagtc
     6781 tctagcaaat taaaaaaagt aaatcaggtc aaggactagt ttggaggcga aaaacatgaa
     6841 aatcggcttt tttaccgata cctactttcc gcaagtcagc ggagttgcta catcgatcaa
     6901 aacattaaaa caagaattag aaagacatgg gcatgaagtc tatattttta caacgaccga
     6961 tcctaatgcg gataagttgg aaaaagatgt gattcgaatg ccaagtgtac catttatttc
     7021 tttcaaggat cgaagagttg ttgttagagg catgtggtat gcttatttgg ttgccaaaga
     7081 actagaacta gatcttattc atacccatac agaattcgga gcagggatac ttgggaagat
     7141 ggtagctaag aagttgaaaa tacctctgat ccatacttac catacgatgt atgaagacta
     7201 ccttcattat atcggtaaag gaaaagtgat caggcaatcc catgtaaaat acttgtctag
     7261 attatttgca aatcatacga caggtgtagt ttgtccaagt gaacgtgtta ttgatacatt
     7321 acgcagctat ggtgtaatag caccgcttcg cgtgattcct actggtatcg acattgaaaa
     7381 attcaaacgt tctgatatca ctcagcagga tatcagcaaa ttaagggagt cactaggtct
     7441 tcaggaaaac catttgatga ttctttcttt aagcaggatt tcttatgaga aaaatatcca
     7501 agccttgatt aatgggtttc ctcaggtcat ctccgcttat cctaacgcca gattagttat
     7561 cgtaggaaaa ggaccttatg tagaagagtt agaggaattg atctcagaac tgcagttaga
     7621 agaatttgtg cagttcacag gagaagtgga caacaatgat gtagcacttt attataaagc
     7681 ggcagattat tttgtcagtg cttctacttc agaaacacaa gggcttactt atacagaagc
     7741 gatggcagcc ggaacacctt gtgtcgtgga aggaaatgct tatttgaacc gattgttcga
     7801 tcatccttca ttagggaaaa cttttgaaac cgacgaagac tttgcaccag cacttattga
     7861 ttatatttcc agtgaagttc ctaaagatcc aaaagtttta gaagctaaac tttatgatat
     7921 ttcatctaca catttcggcg aagctatgat cgaattttat caagacatgg ttgtttatca
     7981 tgaagaagag atgcgaagaa aagaagaaga agactcgatt gaacgaatca aaataaaatt
     8041 aaattcattt aaaagataaa attcaaacat tttttatgaa aaaatccttg tttctagcgt
     8101 atactagcat agaagcaagg atttttgatt tttaggaggt cgaaaaaatg aaacttggtt
     8161 ttatcggaac aggcgtcatg ggaagtgctg tcgcacgcca tctactagaa gcaggacacg
     8221 aagtagctgt ctataatcgg acaaaagcaa aagctgatcc tttagtaaca gaaggagcta
     8281 tctgggcaga tacaccaaaa gcggtagcag aacaaagtaa tattctcttt acaatggtag
     8341 gttaccccaa agatgtagaa gaaatctact atggacagtc ggggattttt tctgcagata
     8401 tctcaggaca tattttagtt gatttgacta caagtacacc aagcctggca gaaaaaattg
     8461 caaaaacagc aaaagaaaaa ggcgcagatg cgttagatgc accggtatcc ggtggtgact
     8521 taggagcaaa gaatggtaca ctgacgatca tggtcggtgg agaagaagcc gtttatgatc
     8581 aagtactgcc tttattcaaa gaatttggta cgacatttac attgcatggt tctgctggga
     8641 aaggtcagca tacaaaaatg gctaatcaga tcatgattgc aggtacgatg acaggaatga
     8701 cggaaatgct tgtctatgca caaaaaaacg gacttgattt aaagaaagtg attgagacgc
     8761 tttcaggtgg aagtgcagct aactggtctt taagcaatta ttctcctcgt attttaaaag
     8821 aagattacac acctggtttt tttgtcaaac attttatcaa agatttaaag attgcattag
     8881 aagaagcaga aaaaatggat cttgtattgc cagcaactac ccaagcattg aaactatatg
     8941 aagagttggc agataaaggt tttgagaatg acggaaccca agctttgatc aaactttggt
     9001 ggcctgaagg aaaaatacca gaaaaaaaat cataaaaaac gagttttttt agagaaaatc
     9061 aatgcaagta ggctatgctt ttgatacaat ataacagaaa taagaaaagg aggacctccg
     9121 atgacacatt cacaattagt cgcaatcata aaacgactgg aggctatggt cgagtcagca
     9181 gataatgaac tgcaagttcg ccgttttgaa aaagaaggcg tagagaagtg tacagtaact
     9241 tatgataaag cgaccgatac atttgaatta actgaaacag atactcatca gcaatacgaa
     9301 tttgataata tcgatatcgt agcgatggaa atctatgact taatccaata aattttagga
     9361 taattgacat ttgaagttga agatgcctaa aagaagccga gaagcaacta atttagttgc
     9421 ttttcagctt cttttttgtt taacagttca atttcaaact gttctttttc taattttttg
     9481 cggtaactga tcaagatttg tacaggatcg atttcttctt ttgacaattg acgtttcaaa
     9541 gataaatgag cttctttctt ttcataatag aagtcaggag aaacgtcacc agaggtaatc
     9601 attgtttctg caagttgacc ataacaaacg aggtagtgct ttttctgact ctcatctgct
     9661 gctaatggca tgctgaaggt gagcgatggt gttcttaaaa actgcttttg ttcctggatc
     9721 cgtctaataa aataagtaac taattcgata tctttttgaa gcataccggc tttttcgaaa
     9781 ttcaactgat agatgaaatg ttgttgtctt tttttcaagt aagtcagtag ctttttattt
     9841 cggccttgaa acagcccttg gagttccttt tttttctgtt caaaagtcat ttcctgtagg
     9901 tcaggaagct ggactcgcaa agctaacaaa ctaatttcag acaaccaagg catttgataa
     9961 gtttcttcca ttatacgcaa aatagacggc atttttttat agagacgaaa aggcccgtat
    10021 gttcgagccg taggagtatc tgttaataca aatcccttat tggtaatatg tacataatta
    10081 taattactga aataattcat ttgtcggtta taaaaaggac ggtaatgttg gatcaattga
    10141 cactcgagca ataaagcatc taattccgtg tctactacga cgaaatcaaa atctgttata
    10201 tgatgaatca tacgtaatac tttttttgaa tgctgtttat tctgatgaaa atagctgctt
    10261 actctatttt tgagccgttt tgctttacca acatagatga ccttaccatc tcggtctttc
    10321 ataagataga cacccggcag catggggaga gttttcattt tttcttttaa cataggatct
    10381 ccttttacgc tttgaataaa aagactataa caaattttga aataatggac aagttttagg
    10441 gaattctata gttctgttca cag
//
LOCUS       DAYYAG010000096        10445 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000096 DAYYAG010000000
VERSION     DAYYAG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.098"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..646)
                     /locus_tag="ACHARQ_002135"
     CDS             complement(161..646)
                     /locus_tag="ACHARQ_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8410535.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    736..1081
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1126..1767
                     /locus_tag="ACHARQ_002136"
     CDS             1126..1767
                     /locus_tag="ACHARQ_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8410536.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1786..2421
                     /locus_tag="ACHARQ_002137"
     CDS             1786..2421
                     /locus_tag="ACHARQ_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8410537.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2439..3254
                     /locus_tag="ACHARQ_002138"
     CDS             2439..3254
                     /locus_tag="ACHARQ_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8410538.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3573..5009
                     /locus_tag="ACHARQ_002139"
     CDS             3573..5009
                     /locus_tag="ACHARQ_002139"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8410539.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5198..7630
                     /locus_tag="ACHARQ_002140"
     CDS             5198..7630
                     /locus_tag="ACHARQ_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8410540.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7860..8927
                     /gene="rlmN"
                     /locus_tag="ACHARQ_002141"
     CDS             7860..8927
                     /gene="rlmN"
                     /locus_tag="ACHARQ_002141"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8410541.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8917..9123
                     /locus_tag="ACHARQ_002142"
     CDS             8917..9123
                     /locus_tag="ACHARQ_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410542.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9120..10223)
                     /locus_tag="ACHARQ_002143"
     CDS             complement(9120..10223)
                     /locus_tag="ACHARQ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8410543.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3444 a         1791 c         2274 g         2936 t
ORIGIN      
        1 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
       61 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
      121 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
      181 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
      241 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
      301 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
      361 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
      421 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
      481 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
      541 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
      601 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
      661 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
      721 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
      781 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
      841 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
      901 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
      961 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
     1021 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
     1081 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
     1141 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
     1201 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
     1261 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
     1321 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
     1381 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
     1441 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
     1501 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
     1561 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
     1621 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
     1681 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt
     1741 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
     1801 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
     1861 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
     1921 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
     1981 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
     2041 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
     2101 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
     2161 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
     2221 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
     2281 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
     2341 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
     2401 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
     2461 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
     2521 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
     2581 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
     2641 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
     2701 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
     2761 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
     2821 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
     2881 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
     2941 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
     3001 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
     3061 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
     3121 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
     3181 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
     3241 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
     3301 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
     3361 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
     3421 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
     3481 acctccttta cataatttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
     3541 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
     3601 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
     3661 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
     3721 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
     3781 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
     3841 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
     3901 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
     3961 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
     4021 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
     4081 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
     4141 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
     4201 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
     4261 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
     4321 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
     4381 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
     4441 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
     4501 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaagt tcatcagaag
     4561 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
     4621 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
     4681 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
     4741 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
     4801 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
     4861 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
     4921 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
     4981 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
     5041 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
     5101 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
     5161 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
     5221 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
     5281 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
     5341 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
     5401 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
     5461 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
     5521 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
     5581 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
     5641 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
     5701 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
     5761 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
     5821 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
     5881 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaatatgaga agaaaaaagc
     5941 ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc
     6001 ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct
     6061 agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga
     6121 gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca
     6181 agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga
     6241 tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg
     6301 tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca
     6361 gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc
     6421 tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat
     6481 tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc
     6541 aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca
     6601 aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa
     6661 gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt
     6721 aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca
     6781 tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt
     6841 acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg
     6901 ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc
     6961 attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt
     7021 tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct
     7081 agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc
     7141 cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg
     7201 ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc
     7261 gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt
     7321 tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac
     7381 gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt
     7441 tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg
     7501 agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag
     7561 caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa
     7621 tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg
     7681 catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc
     7741 ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat
     7801 taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt
     7861 tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa
     7921 acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa
     7981 gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt
     8041 ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat
     8101 atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg
     8161 gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa
     8221 gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga
     8281 tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa
     8341 tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg catatcatca
     8401 atgatgccaa aggattggca attggtgctc ggcacattac agtttccact agtggattag
     8461 cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc
     8521 acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg
     8581 agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg
     8641 aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc
     8701 tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg
     8761 aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga
     8821 agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt
     8881 gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc
     8941 gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat
     9001 aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc
     9061 aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt
     9121 taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca
     9181 agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt
     9241 gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg
     9301 gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg
     9361 aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg
     9421 tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa
     9481 ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg
     9541 acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca
     9601 taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag
     9661 cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca
     9721 atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg
     9781 tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg
     9841 acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt
     9901 ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca
     9961 aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat atttccataa
    10021 gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact
    10081 tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc
    10141 ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt
    10201 ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa
    10261 ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag
    10321 gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac
    10381 gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct
    10441 accga
//
LOCUS       DAYYAG010000097        10343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000097 DAYYAG010000000
VERSION     DAYYAG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.099"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHARQ_002144"
     CDS             6..305
                     /locus_tag="ACHARQ_002144"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8410544.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHARQ_002145"
     CDS             378..1739
                     /locus_tag="ACHARQ_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8410545.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHARQ_002146"
     CDS             1761..2021
                     /locus_tag="ACHARQ_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410546.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHARQ_002147"
     CDS             2035..2787
                     /locus_tag="ACHARQ_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8410547.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHARQ_002148"
     CDS             complement(2881..3486)
                     /locus_tag="ACHARQ_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8410548.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHARQ_002149"
     CDS             complement(3539..4513)
                     /locus_tag="ACHARQ_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8410549.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHARQ_002150"
     CDS             complement(4891..5610)
                     /locus_tag="ACHARQ_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8410550.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHARQ_002151"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHARQ_002151"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8410551.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHARQ_002152"
     CDS             7161..8558
                     /locus_tag="ACHARQ_002152"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8410552.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHARQ_002153"
     CDS             8639..9079
                     /locus_tag="ACHARQ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8410553.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHARQ_002154"
     CDS             9227..9553
                     /locus_tag="ACHARQ_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8410554.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHARQ_002155"
     CDS             9554..10303
                     /locus_tag="ACHARQ_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8410555.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3278 a         1825 c         2143 g         3097 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgt
//
LOCUS       DAYYAG010000098        10309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000098 DAYYAG010000000
VERSION     DAYYAG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.100"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..236
                     /locus_tag="ACHARQ_002156"
     CDS             87..236
                     /locus_tag="ACHARQ_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8410556.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            247..1761
                     /gene="dltA"
                     /locus_tag="ACHARQ_002157"
     CDS             247..1761
                     /gene="dltA"
                     /locus_tag="ACHARQ_002157"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8410557.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1758..2963
                     /gene="dltB"
                     /locus_tag="ACHARQ_002158"
     CDS             1758..2963
                     /gene="dltB"
                     /locus_tag="ACHARQ_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8410558.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3008..3241
                     /gene="dltC"
                     /locus_tag="ACHARQ_002159"
     CDS             3008..3241
                     /gene="dltC"
                     /locus_tag="ACHARQ_002159"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8410559.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3244..4512
                     /gene="dltD"
                     /locus_tag="ACHARQ_002160"
     CDS             3244..4512
                     /gene="dltD"
                     /locus_tag="ACHARQ_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8410560.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4653..5735
                     /locus_tag="ACHARQ_002161"
     CDS             4653..5735
                     /locus_tag="ACHARQ_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8410561.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5735..6538
                     /locus_tag="ACHARQ_002162"
     CDS             5735..6538
                     /locus_tag="ACHARQ_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8410562.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6608..7348
                     /locus_tag="ACHARQ_002163"
     CDS             6608..7348
                     /locus_tag="ACHARQ_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8410563.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7359..8363
                     /locus_tag="ACHARQ_002164"
     CDS             7359..8363
                     /locus_tag="ACHARQ_002164"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8410564.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8408..10252)
                     /locus_tag="ACHARQ_002165"
     CDS             complement(8408..10252)
                     /locus_tag="ACHARQ_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410565.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3311 a         1705 c         2082 g         3211 t
ORIGIN      
        1 aaaaacggtt tctgtaagct agacacatgt ttctttttgt caaaattagt tacactttgt
       61 gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt
      121 attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt
      181 atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg
      241 aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct
      301 gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca
      361 gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt
      421 ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc
      481 tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct
      541 aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc
      601 ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat
      661 agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca
      721 aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac
      781 tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg
      841 gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca
      901 gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg
      961 gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat
     1021 gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa
     1081 agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca
     1141 actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta
     1201 ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt
     1261 tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg
     1321 aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat
     1381 acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat
     1441 cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc
     1501 caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag
     1561 cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact
     1621 aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc
     1681 cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca
     1741 gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt
     1801 tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa
     1861 gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta
     1921 attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat
     1981 ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta
     2041 ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag
     2101 catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca
     2161 tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc
     2221 tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc
     2281 agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata
     2341 tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc
     2401 ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata
     2461 tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg
     2521 gtacaagcta catgatggga tatcagacac caatcaattt caataaacct tttttatcat
     2581 ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt
     2641 atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg
     2701 taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga
     2761 cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat
     2821 ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct
     2881 tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt
     2941 tagataaact tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa
     3001 aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt
     3061 aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt
     3121 tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga
     3181 gcgagaggat tggtgcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg
     3241 agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg
     3301 tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt
     3361 gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta
     3421 tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt
     3481 catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca
     3541 cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa
     3601 aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact
     3661 gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag
     3721 aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa
     3781 gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct
     3841 gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc
     3901 agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca
     3961 aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg
     4021 acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag
     4081 ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat
     4141 tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca
     4201 ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt
     4261 gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc
     4321 aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg
     4381 ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag
     4441 tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta
     4501 caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt
     4561 ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat
     4621 cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta
     4681 ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca
     4741 gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg
     4801 ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg
     4861 gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt
     4921 ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg
     4981 tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac
     5041 cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag
     5101 agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg
     5161 gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc
     5221 gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg
     5281 tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata
     5341 ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag
     5401 cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc
     5461 ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat
     5521 atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg
     5581 ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc
     5641 atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata
     5701 gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga
     5761 atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt
     5821 tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt
     5881 tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt
     5941 cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa
     6001 aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa
     6061 tttgatcaat tcagtcatca actggctact caacagttta atctttctgc ccatgttttt
     6121 aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg
     6181 tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt
     6241 tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa
     6301 gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa
     6361 agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg
     6421 agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc
     6481 agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca
     6541 tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga
     6601 atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg
     6661 gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc
     6721 tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag
     6781 ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca
     6841 tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag attccattca
     6901 ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga
     6961 attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta
     7021 cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg
     7081 ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta
     7141 tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact
     7201 tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt
     7261 agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc
     7321 tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa
     7381 accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag
     7441 atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa
     7501 atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc
     7561 gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca
     7621 tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag
     7681 cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg
     7741 atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg
     7801 gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag
     7861 acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat
     7921 ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga
     7981 aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt
     8041 ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga
     8101 agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc
     8161 atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac
     8221 taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc
     8281 tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag
     8341 aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg
     8401 ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga
     8461 aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca
     8521 ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg
     8581 tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg
     8641 acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct
     8701 ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa
     8761 gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta
     8821 agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt
     8881 cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt
     8941 gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct
     9001 gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt
     9061 gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga
     9121 ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg
     9181 ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag
     9241 ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag
     9301 tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa
     9361 aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt
     9421 accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta
     9481 agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta
     9541 aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa
     9601 gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta
     9661 tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa
     9721 aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct
     9781 gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta
     9841 tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa
     9901 tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt
     9961 cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag
    10021 gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa
    10081 cccacgaatc ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa
    10141 agaaataagc aattcctgtt aaaaataaca atgataatat aatgattatg ctccacttta
    10201 tacgtaaata aaaagattct tttttattat atatgttatt aaaaaaagac ataaaggtat
    10261 tcctttctaa aacttgtaat gttagggtaa acgtttttta aactaacgt
//
LOCUS       DAYYAG010000099        10227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000099 DAYYAG010000000
VERSION     DAYYAG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.101"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            406..675
                     /gene="rpsN"
                     /locus_tag="ACHARQ_002166"
     CDS             406..675
                     /gene="rpsN"
                     /locus_tag="ACHARQ_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8410566.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            690..869
                     /gene="rpmF"
                     /locus_tag="ACHARQ_002167"
     CDS             690..869
                     /gene="rpmF"
                     /locus_tag="ACHARQ_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8410567.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            901..1278
                     /locus_tag="ACHARQ_002168"
     CDS             901..1278
                     /locus_tag="ACHARQ_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410568.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1299..1448
                     /gene="rpmG"
                     /locus_tag="ACHARQ_002169"
     CDS             1299..1448
                     /gene="rpmG"
                     /locus_tag="ACHARQ_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8410569.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1472..2455
                     /locus_tag="ACHARQ_002170"
     CDS             1472..2455
                     /locus_tag="ACHARQ_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8410570.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2485..2607
                     /locus_tag="ACHARQ_002171"
     CDS             2485..2607
                     /locus_tag="ACHARQ_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8410571.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2625..4148
                     /locus_tag="ACHARQ_002172"
     CDS             2625..4148
                     /locus_tag="ACHARQ_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8410572.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4172..4411
                     /locus_tag="ACHARQ_002173"
     CDS             4172..4411
                     /locus_tag="ACHARQ_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410573.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4604..5065)
                     /locus_tag="ACHARQ_002174"
     CDS             complement(4604..5065)
                     /locus_tag="ACHARQ_002174"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8410574.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5260..5478)
                     /locus_tag="ACHARQ_002175"
     CDS             complement(5260..5478)
                     /locus_tag="ACHARQ_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410575.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5596..7212
                     /locus_tag="ACHARQ_002176"
     CDS             5596..7212
                     /locus_tag="ACHARQ_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8410576.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7453..8793)
                     /locus_tag="ACHARQ_002177"
                     /pseudo
     CDS             complement(7453..8793)
                     /locus_tag="ACHARQ_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9231..9377)
                     /locus_tag="ACHARQ_002178"
     CDS             complement(9231..9377)
                     /locus_tag="ACHARQ_002178"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410577.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9416..9610)
                     /locus_tag="ACHARQ_002179"
     CDS             complement(9416..9610)
                     /locus_tag="ACHARQ_002179"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8410578.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9520..9852)
                     /locus_tag="ACHARQ_002180"
     CDS             complement(9520..9852)
                     /locus_tag="ACHARQ_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8410579.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3412 a         1615 c         2015 g         3185 t
ORIGIN      
        1 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
       61 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
      121 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
      181 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
      241 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
      301 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
      361 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
      421 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
      481 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
      541 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
      601 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
      661 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
      721 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
      781 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
      841 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
      901 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
      961 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
     1021 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
     1081 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
     1141 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
     1201 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
     1261 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
     1321 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
     1381 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
     1441 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
     1501 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
     1561 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
     1621 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
     1681 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
     1741 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
     1801 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
     1861 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
     1921 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
     1981 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
     2041 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
     2101 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
     2161 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
     2221 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
     2281 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
     2341 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
     2401 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
     2461 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
     2521 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
     2581 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
     2641 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
     2701 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
     2761 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
     2821 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
     2881 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
     2941 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
     3001 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
     3061 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
     3121 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
     3181 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
     3241 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
     3301 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
     3361 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
     3421 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
     3481 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
     3541 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
     3601 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
     3661 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
     3721 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
     3781 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
     3841 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
     3901 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
     3961 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
     4021 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
     4081 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
     4141 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
     4201 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
     4261 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
     4321 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
     4381 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
     4441 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
     4501 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
     4561 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
     4621 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
     4681 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
     4741 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
     4801 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
     4861 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
     4921 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
     4981 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
     5041 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
     5101 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
     5161 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
     5221 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
     5281 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
     5341 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
     5401 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
     5461 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
     5521 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
     5581 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
     5641 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagaa aattgctgtc
     5701 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
     5761 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
     5821 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
     5881 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
     5941 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
     6001 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
     6061 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
     6121 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
     6181 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
     6241 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
     6301 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
     6361 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
     6421 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
     6481 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
     6541 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
     6601 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
     6661 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
     6721 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
     6781 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
     6841 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
     6901 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
     6961 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
     7021 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
     7081 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
     7141 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
     7201 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
     7261 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
     7321 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
     7381 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
     7441 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
     7501 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
     7561 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
     7621 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
     7681 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
     7741 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
     7801 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
     7861 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
     7921 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
     7981 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
     8041 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
     8101 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
     8161 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
     8221 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
     8281 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
     8341 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
     8401 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
     8461 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
     8521 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
     8581 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
     8641 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
     8701 attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc
     8761 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
     8821 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa
     8881 gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct
     8941 tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct
     9001 tctaaataat cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg
     9061 attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc
     9121 ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa
     9181 gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt
     9241 tgtaatggac ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga
     9301 atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg
     9361 taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac
     9421 atttttttac catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa
     9481 atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt
     9541 cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt
     9601 cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc
     9661 tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata
     9721 ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt
     9781 ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata
     9841 tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta
     9901 tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag
     9961 tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac
    10021 atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag
    10081 gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat
    10141 acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac
    10201 gaattaaaaa acgttgataa ttaaaat
//
LOCUS       DAYYAG010000100        10169 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000100 DAYYAG010000000
VERSION     DAYYAG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.102"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..672)
                     /locus_tag="ACHARQ_002181"
     CDS             complement(<1..672)
                     /locus_tag="ACHARQ_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410580.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFY"
     gene            complement(795..2345)
                     /locus_tag="ACHARQ_002182"
     CDS             complement(795..2345)
                     /locus_tag="ACHARQ_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8410581.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(2347..2763)
                     /locus_tag="ACHARQ_002183"
     CDS             complement(2347..2763)
                     /locus_tag="ACHARQ_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410582.1"
                     /translation="MNNTIPYGYVIKNGEINVDEQQAQQIREFFNIYLEGVGLQEAGK
                     RSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVP
                     DTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            complement(2760..4313)
                     /locus_tag="ACHARQ_002184"
     CDS             complement(2760..4313)
                     /locus_tag="ACHARQ_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8410583.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(4374..4547)
                     /locus_tag="ACHARQ_002185"
     CDS             complement(4374..4547)
                     /locus_tag="ACHARQ_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8410584.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            4842..5147
                     /locus_tag="ACHARQ_002186"
     CDS             4842..5147
                     /locus_tag="ACHARQ_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410585.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(5208..6245)
                     /locus_tag="ACHARQ_002187"
     CDS             complement(5208..6245)
                     /locus_tag="ACHARQ_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8410586.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(6361..7269)
                     /locus_tag="ACHARQ_002188"
     CDS             complement(6361..7269)
                     /locus_tag="ACHARQ_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8410587.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(7259..7678)
                     /locus_tag="ACHARQ_002189"
     CDS             complement(7259..7678)
                     /locus_tag="ACHARQ_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8410588.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(7679..8086)
                     /locus_tag="ACHARQ_002190"
     CDS             complement(7679..8086)
                     /locus_tag="ACHARQ_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM8410589.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(8073..8240)
                     /locus_tag="ACHARQ_002191"
     CDS             complement(8073..8240)
                     /locus_tag="ACHARQ_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410590.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(8250..9719)
                     /locus_tag="ACHARQ_002192"
     CDS             complement(8250..9719)
                     /locus_tag="ACHARQ_002192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410591.1"
                     /translation="MSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTS
                     DNIVLLYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGI
                     YNSSNTPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYP
                     TTTRQDYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTT
                     IDSAVISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADK
                     IAANILTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISG
                     HSDWRGLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYL
                     PSMLRIGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIR
                     EICAKLAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
BASE COUNT         2746 a         2326 c         1772 g         3325 t
ORIGIN      
        1 ataaaatggc tgctgaagtt gtttagaaac actcgtgcct ttacttaaat tagtttcttt
       61 tagttttact cgaattaatg tatcaaaacc agaatcaatt ttgtttattg gttgaggaca
      121 gttcattatt ggaaaacata aatcataatc ttcttttttc caatttttct tctgctcaaa
      181 aattaattca ctgaatttgt cagaatctct tgatacatac tgctcaaaaa gcttttttgt
      241 gaattcttca ctaaatcgaa tagcaaattc tcccgaatac agttcgaccc actcagcatc
      301 gtttagcaag gaacgaaaac cagtcccctt atttccgata ttttgtgatg atttattact
      361 cgctcttcct aagcacaatc tttcaattgt atcttttgaa aatgaagtgc ctgtattccc
      421 aacttccaag atattatttt tatatgttat ctgaactttc acttcgtcat tgctagcatc
      481 attttctgat tttatatctc cataggcatc gtctacattc tgtaataact ctaaaatctg
      541 acgcccatta taatcacgca tcaattcata ttcttcgctg caggcctttt caagttcatt
      601 actcaactca gaatccgtat atttacgaat aaaaatatcc cttaattcat caatggtatt
      661 ccattggctc acagaacttc gcccccagcc tattttttac ataatatatt atatcatcaa
      721 tcactttatg aaatccaatc aataaagacg ttaatatggc tacccacatt aacgtctagt
      781 cctatctatt caaattaaat ttctacagtc actctcaccc cacttttgaa atcaacaaca
      841 atgttctttt caaaaatggt gaccttctca atcaaccttc gaacaagcac ttcattatac
      901 tcattgatga catcgtgttc ttttacaaag actaccatct catcaacccg ttgttgcaaa
      961 ttagtattcg ctgctatttg ttttgcaacc gcatctcgtt catctcttag tgaaaatatt
     1021 tggcttgcca attcatcgta accctgaccg ccaactgcca ggctaataat ttcagcttca
     1081 agctctttca actgactgtc tagttctgcc aatcggtcag ttaatccatc agtcataact
     1141 gagttgatat tgtcatttaa ttgctgaaga aaagcatcac gatcgattaa cgtttcattg
     1201 attgccttga taacagctcc atgcaggtct gtttcgtaga ttgttcgacc cgtgcaatta
     1261 tgttctcgtt tatccactct ggatttgcat cgccacacag ttcctcgatt cttccaatga
     1321 actctgcggt aagcattgcc acagtcaccg caaactgtga taccagataa cgcatacttc
     1381 ccagaaaagc actgtgactt tttgtttttc attgatttac gccggtctag ttcttgctgg
     1441 gcttgcatga agatatcttt tgggataatg gccggatggc tatcttcaac ataatactgc
     1501 tgagcgtaac tcccgtcatt cttcactcgt ttctttgtca agaaatcggt agtatatgtt
     1561 ttttgaagca gcgcatcccc catgtacttc tcgtttcgaa gaatcaatct gactccttct
     1621 cctcgccaga gctttcgctt ccctcccgtt aggatgccat cagccattag accattagca
     1681 atttcttgca aggaactgcc ctcaaggtat tcgcggaaga tgcgtttgat gacttctgct
     1741 tcctttggtt caatcaccaa gttaccatct tcgtccttag tgtacccaag aaaatgattg
     1801 tggttgatac gtagctgtcc ctgctggaaa cgatactgta cgcccatctt ggtgttttga
     1861 cttagtgact ggctttcttg ctgggccaga cttgccataa tggtcaaaag tacctcaccc
     1921 ttcgcatcca ttgtattgat gttttccttt tcaaaaaaca ctgcaatctt cagttctttt
     1981 aactgtcgta tgtattgcaa gcaatcaacc gtattacggg caaagcgtga aattgatttt
     2041 gtgatgatgt actcaacttc gccgttttga cacgcctcaa tcatccggtt aaaaccgtca
     2101 cgctttttcg tcccagttcc tgaaatccct tcatccgcaa atattcctac aagctcccaa
     2161 tcgggatttc tttgaatgta gtccgtatag tgttgaacct gtgcttcgta agaactcgcc
     2221 tgttcctcca tttcagtaga aacacgacaa taggctgcca ctttcaactt tggtttctct
     2281 tcggtattct ctgctgccca ttgtctaggc ttcattggaa taaccgtcac ctgttttcct
     2341 gccatttcac ttaacctcct tgatttttga atatgcatat tgcgcttgtt tgaatgggtc
     2401 agaatacttt tcggtaatct tgccagattt aaaatgagtg ggtattacct tcgtatctgg
     2461 cactttgatc tccttgcgtt ttttaaagaa cttaattctt cgttcttgct ccagatccgc
     2521 ttggaagaaa atatcctcat caatgatttt tggatatatc tcatttccta agtaaacctc
     2581 attcatcaaa atacgtttca taaagggatg cggcttgtta atgccagagc gtttgccagc
     2641 ttcttgtaag ccaacaccct caaggtaaat attgaagaac tcacgtattt gttgagcttg
     2701 ctgttcatcc acgttgattt ctccattctt aatgacatac ccgtatggaa ttgtattgtt
     2761 cattctggta acctctcttt caaaagtagt ccgcacttaa gttcaaatcc gacttcttgg
     2821 cggttgtaaa ctgtaatgct gtttgtgaac ttcttgaata aatcttcatc ccagtccgtc
     2881 aacatctctg ccatccgcgc gaatttgtat aaggcttcta gttcaatttg ctgagtgctt
     2941 ttgccgttga ctgactcaat gatgaggcgc tgcttttctt gtgcgacagc cctctcttgt
     3001 tgaagtttgc tttgttcttc taaagacacc ttcgttgata aaatactgga agcagtcaaa
     3061 cttgccagaa cctcaagctt ttcttgaatc ttgtcgattt catcagcaag cacttcgagc
     3121 ccttcctgat tcgcacctgc tttattcaaa gacttcataa aaggttccag aaccaacttg
     3181 tgtccgaaaa tcaacttatt caacatggtc aaataagcaa ccttcaaatc ttcatccgta
     3241 attggtaatg catcgcagct atctttattt ttaagatgcc ccgtacactt ccactgaacc
     3301 ttcttcgtat tacctttcca ataagtgtga acccgcttga atttgctgcc gcatttatca
     3361 cagatgatgt tgcctgaaaa tgcgtatcgc ttggtgtact ttttcccgtc accagtattc
     3421 cctttggcct cagcttttct ccgaatgagt tcttgaactc ggtcgtaatc ttctttagat
     3481 actatgggtt cgtgattgtt tttgatctgg tacattggca attcgccctt gtttatgcgt
     3541 cgtttgtact ggtcatcaac aaaggtttgt tgcatcgtca aagtcccggt ataaacttca
     3601 tttttcaaaa tacttctgac tgatgttgct tgccaatttg ccccacgttt agtgaattgt
     3661 tttccatcgt ttaaccgctt ggcaatcatt ccagtcgaaa tgccatctaa tgcccactgg
     3721 aacatttgtt taacaacttt tgcttcagct gcaacgatgt gaaaatcata gcctttccgc
     3781 ttgtagccat acggcgggta agctttgaat ttccctttag acatccggtt tcgaatcgcc
     3841 cacttcaagt tttctgaatg ggaataggat tcactttctg ccaagcttga taaaattgaa
     3901 agcaacaact cgctttccat atcaccagta ttaatgtttt ccttttcaaa aaagatggtg
     3961 acaccaatat cagttagctg ccgaaccaga ttcaagcagt caactgtatt tcttgaaaat
     4021 ctggaaatag actttgtcag aatcaaatca atctttccaa gctctgcatc cttaatcaat
     4081 cgttttaaac catcacgttt tacagtcttg gttccagaaa tcccttcatc ataataaaca
     4141 ccgacatagt cccattgttc atggttcttg atgtgatttt catagtgagt aatctgggtt
     4201 tgtaaacttg ccagctgttc attacttgat gttgatacac gagcgtatgc tgccaccttt
     4261 agtttggaag cttccggcaa ctctggacgt gccaatttat gtatcgtttt catcagctca
     4321 actcctttcc attaggtgac atgttagctc taaagtgccg ttatatcaac gtttcagacc
     4381 attatccttg ataaaaaagg attgaatgtt tcccggtttt tattgtcgat tttgacgtat
     4441 tcttccaggt tgattttacc ggtaacaagt aagttcagaa ggatctgctg tgaaatgtaa
     4501 taggctgctt catctgaata gttaatttct gttttcttgt tagtcattgc tttcgcctcc
     4561 tcaagataca ggcgaagaaa atccatgaaa gtaaacccaa tcacaaattt tcctttgcct
     4621 ttcatccata tgcgaaagaa ataaccgact cgaacccaca aaacgaaaaa agcctgcaag
     4681 aatgaaacga atcactcttg caggcttcta agtttgttta tatatagtag gaattattct
     4741 ttgtaaattt tgacttttta cttcccaaac atcaaataag cctttacaag cttcatccaa
     4801 taaataacaa tataaatatc aattgtggga aggagaaagt tatgagtagt tttgatatgg
     4861 aagggttaga aaaagaatat gaacattttt gcaaatctgc gacaacaaag gaactggagg
     4921 aatttgatga aagcttcaaa caagaatttg aaaagtgctt taaaaccata gaggctgaag
     4981 aagctttata ccagttttta caagccaata taaacgcgat gaataatcgt atggaatcaa
     5041 tagaatcgcc tcttttctgc atcaattaca aagagcaggt atttcagtta agaaatcatt
     5101 taatcctaat gcaaaatgct gtaaacacct accttaaaga aaactagacg aaaaaaaggc
     5161 cccacaataa aacttgtgag gctgttttga attataaaga cgtcaaacta cttattgaaa
     5221 attttgtagc tcgttggaac catcgtgaag ttttcttcag taattgggcc atcataaatt
     5281 ttgattttgc aaacttttga cgccacatca aggtcgtgaa caaagtaaat tttatatggt
     5341 gtatctccaa gggaggcagc aacctcatta gtactcattt caaatccgtc agagaagttt
     5401 gtttttgaag ctttcacttc aatatggatt tcattacctg attggtcaaa cgcacaaata
     5461 tcatagccat gaccatctcc ttcagtttta gaaacatgtt caggaagttt tgttttgttc
     5521 ttttctgctt cctgaatgag gaaatcaagt acgatttctt cgcctagagc acccgttttt
     5581 tgttttgcct tgttgacacg ctcccaatca gtatggcgtc cagtgaattt tcgtttctga
     5641 gtcccattcg tgcgatttgg tcccacttca tcaacaagaa cagtaaatcc ttttgaactt
     5701 tcttgctcaa tttcttcaac ttcctcgttt gtaagatcca gttcagacgg caacgattgt
     5761 gtatcatcga ttgggataag atgacgattt tcaatcaatg caacaaaatc ggcataggta
     5821 atttcattca ttccatactg atcccagaag taggcccatg aattcggctt tttacgcttc
     5881 tttttgctag gtgtattcca atcgaagttt tcaataaatg aatcaccttg tttaattgcc
     5941 ggttcgattg taaatgcgtg tgtaattact gccgtaacat ttccagcttc atcaggttca
     6001 gaaatgctct caacgtatcc gcccccgtaa atttcccact tacggtcttt agtaattttc
     6061 cccggatgac gattcaaaac aaccgcgccg gctttcaatt taccatattg gcgcatgttc
     6121 caaccgtaac ttttagcatc atcagcgtat ttcaactgac catcatcttc tgttttcccg
     6181 tgatctaaaa tgaaagcttg ttggctttct gttggttctg gtaacaaatc tttaaacgta
     6241 accatatcga tacctctttc tataattcaa tcggaacaaa tttatgttct ggttgaatta
     6301 ataataacac gttcgatatt ttaagtcaac aatccggaca taattttgtt ccgattaaat
     6361 ttatgaaatc aaaatataat cgtcagattc cgtcttacca gtattcttac cgacagcaca
     6421 atatccaatc gggcaacgag cccataccca accatccgca atcgtcatct tctctgactt
     6481 cggaagcgtc caagttgcgc ctttattgta tgaaccaaca accgcaccac ttaaacttgg
     6541 cttgttgcga acgttgagac cactcacttg aaccacaaga gttttcacgt tggatgttga
     6601 tgacccagat ggataaactt gattaccgtt ctggtcgaac accttataac cagaatggct
     6661 atctgcagcc cgcttcgcat tgttcaagtc cttataggca ccaatttgtg acttggcgtc
     6721 agcccatgat ttgcgaacac gatacagacc actagctggc gtgcttgggg ttgatgaacc
     6781 attattaatc gcattggctt tatccgcaat ctcctgcagt ttaccaagca actgtcccgg
     6841 acatgatgtg gccgaaaaat gtgagtgagg gaagatattc ttgttcacca ccaatttccc
     6901 tagaccataa cgtttggcaa tatctgcaca caactttgca cttgcatcaa tagttgcttg
     6961 attgataccc cagttcggtg atccagtgct attgcagtgc tcaatcccga tacttgagat
     7021 attggcgttc caattaccag cgtgccaagc tgtgtttcct tcgtccacgt atgctcgaat
     7081 ttctccattt tttccaatac cgtagtgtgc tgacgcctca cgcgttttcc aaatatcagg
     7141 aacaatatca tagttggtgg ttgccatgtg atgaatcact atcttatcta ctttggcacc
     7201 actacgacca ttcgagtaat ggctaaatga ctgaaatcca tattttgttg ctagattact
     7261 aattcccatt ttctgtttcc tccttctttt cgtgtaactg ctgcaagatt tcacgcaact
     7321 tttccggcac tggcagtcca atcgccacgg cattctccag cagtgaaatt ccttcgtttg
     7381 agatatagaa gaaaatgacc gcatcacgca gtacatttcc acctttgatg acttccacat
     7441 caagcatatg agccacacca accaaaagaa aaatcatgat tttcttagtg atgccctttg
     7501 ccccaatctt gctggatagt ttcttttcgt tgattgcacg aagcagacca gttacatagt
     7561 ccactaccac aaaagttaca agcgtataaa tcaaaccatc aattcctccc acataccatc
     7621 caatcactcc tccaccacct gcgatggcga gttgaatcca attccacact tctttcattt
     7681 acaaatcctc caattgttct aaaattaaat cgagcatttc agcatctttc cctgaaatgg
     7741 tgacttcact attttcaaat ccagcaacca ataaaggcag cttgtcttga agttcatcca
     7801 cgttgatgtg ggtttcttcg ttgagcaagt ctgccttttc tttgtgatat tcagttgcgg
     7861 tttctgggat gagtgaaaag gcaccgtcat gttggaccaa attgccgtgc tcatctttct
     7921 ttccgtattt ttgaagcaag tctacctcgt cttgatagag ttcatcaacc ttcacctgga
     7981 tgagttttgc aagcttcgtt cgcactcggc taatctttgg tggtaagtca aaacctttta
     8041 agaaactcat cacgtccacc aaatcttcat ttctaatttt gagcattatc tatcttctcc
     8101 tttaaatcac caatctggac acaaagatca tccacgacca tcacaagcaa atcaaccata
     8161 tccgtcccaa ctttcattgg ctttggaata tctgcctcga taggttcagt tactatcact
     8221 tcttcttttt cttcgttcat ggcggcctcc taactgtatg tggtcattga attaaacgac
     8281 tgcggattgt accaaccact cgctgtaccg ttgctactga aaccggttgg taaagcgatt
     8341 gttcgtcccg caagtttggc acagatttca cggatgactc caaagtcaac ccacttccca
     8401 tcatctccga gcatcagatt agaaccgttg aatgcaatac cagcgctgcc ggaacttgag
     8461 ccaatcttag tataaaggga actactaaat gtaatcccac caacagatat ttgatgttgt
     8521 gtccctatac gaagcattga tggtaaataa atgtcgtcgt caaagtggaa ccccttctcc
     8581 ataccagact gcaatgttct cgcataccaa gaaagcttct gggtgtagac actcgcagag
     8641 gaggaatctt tatgagccca tgacatatac tctgttccat actcaagatc aaataccaat
     8701 ccacgccaat cagagtgacc actgatgtta tttgcaccaa ttgttccgac tgtcgttcca
     8761 ccacgtcgaa tggtcattcc ctgcgagttt atttcagtca caagagtgct tcctgaataa
     8821 tatccaatcg tagttcctgt gattcgaatg gacgaactcg cagtcaatgc tgttaggata
     8881 ttagcagcaa tcttatcagc cgtaatcgag ttcgcgccaa tacgggctgc cgacaaagtt
     8941 cctgtggtaa tcttcgccgc atcaatgctt gcaatttttg cactttgaat tgtggcatct
     9001 gcaatctttg cgtttgtgat ggccgcattt tggatcatgg cagaactaat cactgcacta
     9061 tcaatggttg tttgaccagt gatgtgcacc ttgtttccag caatcaaaat actttcaggt
     9121 gagatattga tttgattaat cacgtctcct ttaccaactc gtaaattgat taagtctttc
     9181 tgcatgagaa ttgctgaata taatgtgttg gcattggtat agtcttgcct tgtggtcgta
     9241 ggataaatat ttccttcata ttcatcgggt ttgatgctcg tgttcactcg gactcgcatt
     9301 ttcaatgtgc cactcgggac tgtaattgct ccgctcgaac cagtataaga gatttgatga
     9361 taacttgaac catccgacgg gtttagagta aaaggtgtgt tgcttgaatt ataaatccca
     9421 gcatatgtgc cagtcttaac tgaaaagaaa gctagataca cgctgtcagt agtaatccct
     9481 gatatctgct cagccgttaa cgtccaagaa taagtggcat tgctgctact tgtaacggtc
     9541 gaaatctgtt cccaagcacc acctgtataa agcaagacaa tattatctga tgtatccttg
     9601 cgaccattcc acgaaattac actcggagcg actttgcctt ccaatggcaa tttgtaaatg
     9661 accagataat ctcctgaatt tgaatagggg tttgttgtca cgccacttgt aatactcatc
     9721 gattcaacgt tcttacttac tagataactc gcaatggtcg cattagtata agttcccgcc
     9781 gttagggttt cactaaattg ttttgccgtt gggacaaaat caatgtaagg taaatccatg
     9841 ccgtagtaat gatagtagaa agcagcttga gttccgtcac gcagttgccc gatatactta
     9901 tctcccactt tgacatcaag gaactcactt cggagataat tggttgcgga cacttctgaa
     9961 ccgtcagtgg tactcaaact tccttgttcc cattgattga ctttaaatgg aacgccgatt
    10021 gcttctttgg tttctgaaac acttaacagt aaggcatcag tggtcgctgt aagttttacg
    10081 acgttactta tctcggataa atcaacatag ccatttgtca catccgtttt caacatatag
    10141 caactgatgg tttctggtgc atcactagt
//
LOCUS       DAYYAG010000101         9807 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000101 DAYYAG010000000
VERSION     DAYYAG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.103"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..3385
                     /locus_tag="ACHARQ_002193"
     CDS             107..3385
                     /locus_tag="ACHARQ_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8410592.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3926..5833)
                     /locus_tag="ACHARQ_002194"
     CDS             complement(3926..5833)
                     /locus_tag="ACHARQ_002194"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8410593.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5963..6838
                     /locus_tag="ACHARQ_002195"
     CDS             5963..6838
                     /locus_tag="ACHARQ_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8410594.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6918..7892
                     /locus_tag="ACHARQ_002196"
     CDS             6918..7892
                     /locus_tag="ACHARQ_002196"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8410595.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8072..9217
                     /locus_tag="ACHARQ_002197"
     CDS             8072..9217
                     /locus_tag="ACHARQ_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8410596.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9354..9698
                     /locus_tag="ACHARQ_002198"
     CDS             9354..9698
                     /locus_tag="ACHARQ_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8410597.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3198 a         1805 c         2063 g         2741 t
ORIGIN      
        1 tttttataaa atacacttat tataattttg ttttttattg acagtaaaaa taaattaggt
       61 aatactttaa gtaaacgctt tctaatttgt tacgggagga atacacttgt taaaaaaacg
      121 tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg ttttaatttc
      181 tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg cacacgtgaa
      241 cattgaaggg atgtcaacaa tggaggaaat accaagaact agtgaagata tggatacgga
      301 agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa agcaatctga
      361 agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg ctagtaggta
      421 tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg gcggaaaatt
      481 ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca aagacatcaa
      541 ctttaggatg cgggcgacga tcgaaataga atcttggacc tatacgaatg cccaagaagg
      601 atttgcttta atggtaagag acgcagtacc aaaaaatcat ttgtttgggc aggcatttta
      661 ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact cggatagaca
      721 agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg ggacacgggc
      781 aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga gtgtctcagt
      841 ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag gttcttataa
      901 tatattcgga aacggtcatg ggaatctgtt tccggccacg aactcgatta ctaaactgga
      961 cgtagagatg aaaaaaacga atactggttt agaggcatat tattacgatc ctgaaacagg
     1021 agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt cagatgaatc
     1081 ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag aaggaatcga
     1141 agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta ctcgcttgat
     1201 tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc atactatgtc
     1261 ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag gagaggtgct
     1321 taagaacgca aaaaacttgg ctatcactgc gaatcgggaa tttgattatc agacaaaact
     1381 gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact atccgccaga
     1441 agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag tggactaccg
     1501 taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag gaaacggaag
     1561 cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg ggcagacaat
     1621 cgtcatggcg cctggaactt attccttcga aagaggattg acgattccaa aaggcgttaa
     1681 cgggacaaga gaaaacccga ttcagttgat tgcagagaaa aatccagaaa acaagcgtcc
     1741 tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact actggggttt
     1801 acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga tttctggaaa
     1861 tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa tccaaatcag
     1921 cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca agaactgtac
     1981 atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg ccaaactgac
     2041 aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag atgacggttg
     2101 ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca aaaactcagt
     2161 tgcttatcga aacggctggg taccaggaat tgagggagaa ggtaacggta atggattcaa
     2221 aatgggcggt tcttctttaa cgggccctca tgaattgatc aatagtttgt ctttcgaaaa
     2281 tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata gcagtacaag
     2341 ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa acacagacta
     2401 ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag agcagatccg
     2461 cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt ggaatgaacg
     2521 agagaaacaa tcagaaaata ctcttggaca taccatatca aaagattgga tcgagtctct
     2581 aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg gaagtatcca
     2641 tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg aaagagtgga
     2701 tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcaccgt cttcggaata
     2761 tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac aaaataatcc
     2821 gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata tcattgaact
     2881 gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc ctgtctttga
     2941 agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa gtgctcaatt
     3001 ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc tagaagtcac
     3061 ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat ctgttaccca
     3121 gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg acaaaccaga
     3181 aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac cttctcatcc
     3241 ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa aaacgggaga
     3301 atttaataac ccgttgctag cattagctgg aggaattttg ttaatcggtg tagttgttta
     3361 tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt ttatgatgta aaacaattga
     3421 ctgaggaaag taaaaataga aggaaatgag accaatagat gtctcacttc cttctatctt
     3481 tttatagatg aggttctcct tgctgctttt gattggctgt tcttattact tgatgcagga
     3541 cagctttttc acaatctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
     3601 caacaaacgg caaaatatga ggaactattt ttccgttttt ttctctaata ctcaggtcag
     3661 gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa gccagtttct cggtagtaag
     3721 tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac tacttgaaac
     3781 taagcagctt tttcacaacc tctaatacat ggtattccag aagtaacttc ttcgaaaaat
     3841 aagccgaaat gtcacaaaaa tttgagaagc gattttccca aattccttct tatttcttga
     3901 agctgaccac ttctggcaca acctcttacc agattcgcac tcttttttct ggcgctagat
     3961 acatagcgtc ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg ttttttggtt
     4021 gcacgtttgc tcggagttta gcaggggcat ggacatcgat ttgcagtaaa agttgttggt
     4081 attctttttt tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat tcctcgatag
     4141 aaagatctga ttccgttttt gctgcttcca atgcacagct tagaccgcct gcatcagcaa
     4201 tattttccga aacggttaat tttccgttta cttttccgtc agcaaaaggc agaccatcga
     4261 attcttcgat cattgcttgt gctttgtttt gaaattgtgt aagatcttct tctgtccacc
     4321 agttgttcaa gttgccatat tcgtcaaata aagcgccgtt attatcaaat gcatgggaaa
     4381 tctcatgagc gataacagct ccgattccac cataattagc agagcttgat tgttctagac
     4441 tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat cacattccga aatggatgat
     4501 aataagcatt gaccgtgtca gcactcattt cccattctga acgatccact ggtttgttcc
     4561 atttgctgaa acgatctttt aatgtgagtt tgctgaaaac aagagtattt gaaagcaaag
     4621 taccaccctg atctgcaggt actgttttga actggtcgaa taatgctggg atcttatcgg
     4681 ggtagcctac ttggatacct aagttattta attttatgac ggctttttct tttgttgcat
     4741 ccccaagcca gtcattttct tctagacgtt ttttataaac ggcgatcatt tttttcacca
     4801 tttgttctac gtctttttta gcagcttcgc caaaatattt ttttccgtag taatcgccaa
     4861 ctacttgatc gaattgtcca gaggcaaggt aatacgctgc ttttttctga ggcattgctt
     4921 cgtctgttcc agacaatacg cgagaataga ttccgctgac ttggcggaat tcttctgata
     4981 gataaccact aagagaacgt actgttttaa tgatcatcca gcttttgaat aacggaaaat
     5041 gagcttctgt caggatgtca ttcaaatgat cgaagtacac tggatcagtt acgatcactt
     5101 tatctggagt tgtcccaatc aaacccgtga caagtttttt caagtccagt ttatctgtgt
     5161 atttaataaa tgtatcaaaa gattgcggat tatacatttt gctgtaatct gcgttttctt
     5221 ctgcactttt tacatgagga gcaatcaatt tatcaaattg cactgcttgt tccgtgattg
     5281 cttctgcctg acttttttct ttgccggcaa gctctaataa ctgaacagcc atatcaaaga
     5341 aaacgcctat tagttgtgca ccgttgggat ggtctgatgc ataatacgtc ttatctggaa
     5401 ggaataaaga aggcggataa gcaaagaagg cgttcgtttg cgcattcttc atatctgcat
     5461 ctacatccag attaaatggc aatggtaatg tatccaacag ccattctgga aattgcttat
     5521 tcaattcgtc gaaagactgc aatgattcaa ctttttcgat tataggttga agcggttgag
     5581 catgcaactc atttcttcgg tcataatcgt ttgccagttg ataatatgca aggaaatgtt
     5641 tcattttttc tgaagacact ttttctggat gggcaagcag atcgtctgtt tctcccatca
     5701 aaagttggtc gatcccgtca actaaatctt ggaaaccgcc tgtagctggt ttatctgcag
     5761 ggatttttgc tgttttttgc cattcttcat tgactgcttc atagaaatct tgtttcaatc
     5821 gttctttgtt cattcatcga tcgctccttt tttgtttcgt gtgcccatta tcttacaaaa
     5881 tgaaggaatt aacaaataga acataaaaaa ttgaaaattc gaacaatcaa actgatttgt
     5941 ttttttcaat ccttcactta taatgaaaaa aaggaggcga tcccaactga atcaagtagt
     6001 ggataaacct ttatatttca aagtagataa aaaaattaaa aaattagcca gcactcagca
     6061 gctgcagtca agaaaaagta aattgctttt tttagctctg gtttttgaag atcaaagtta
     6121 tgtgattatc gatcaatcag gacatccggt agaatattct ccagcaaaat atacttatca
     6181 agagggtctt tcttgtaaaa aatggaagct gatcaatgaa gagccaatcg agttatctag
     6241 atggatcaac agaaaagaag atattcccgt tttgatcgaa gaaaaaaggt caggaaagga
     6301 actcgcaaat tgctgggtgg gattgccaga agaacgtttt ttgaggtata agaaatgggc
     6361 taccccctca ggctatcttt gtggtactta cgcggcagca gttctccttg cctattatca
     6421 agactatcgt aaggggtgga tgcttccaaa tgagattcgc aagaaaaata cggctgattc
     6481 gttggttttg acgaaggcat tgcgtagcca gatccaacca ctaggacttc ctacgattcc
     6541 ttttcaggtt agtacaggga tttctagttt tttaaagaag aatggcaatc atgagcgtgc
     6601 acgagcaact cttctaggtt catggcagcg agctacaaaa cgtatacgtg aaggcaaacc
     6661 tgtgatgatt ggtattttga aagtacttgg cagtacatat ggcaatcatt gggtgacagc
     6721 gtacgcttat tttgaaactg aaacaggaga acgttattac aaagtacacg acaactgggg
     6781 cgattatcat aaagtcattc ctgccagttg gagtaacgga acagtttcac ttccttaaga
     6841 atttgctgaa attaacagaa aatcttggaa aaatatgtta aactatcgaa gaatagagaa
     6901 gttttgggga ggactacatg agtaataagt atcaggatga cacgttgaag atttttagtt
     6961 tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt gttcggaaca gaattaggta
     7021 aaagcgtcgt gaagcaattt agtgatggcg agatttccat caatatcgaa gaaagtattc
     7081 gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc tgtcaatgat tactatatgg
     7141 aactactgat catgatcgat gcaatgaagc gagcaagtgc gaaaacgatc aatgtcgtat
     7201 tgccttacta tgggtatgct aggcaagacc gcacggcgaa acctcatgaa ccgatcactg
     7261 cgaaattgat cgctaacttg atcgaagaag caggggcaac tcgagtactg actttagatc
     7321 tgcacacggt tcaagtacaa ggattctttg atattcctgt agataattta tttacgatgc
     7381 cgttgtttgc tcattattac cgtcaattag gtctgacggg agatgacatc gtagttgttt
     7441 cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc agaatatttg aacagcacat
     7501 tagccatcgt agatcatgca gatgatgctt caactgaggg tggttatgtg atcggtgatg
     7561 ttcaaggaaa gacatgtatc atggtagatg atatcttgaa cacaggagca acgcttgcac
     7621 gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt atatgcctgt gcgtcacatg
     7681 gattattgtc cgatcatgcg aaaagtattt tagacaatgc accaatcaaa gatatttgta
     7741 tcactgattc tgttttcaca gaagaaaatc gtcatccaga aaacttaaca atcgttacat
     7801 gttcagaatt aatgggtgaa gcggtcaaac gcatccatga aaatacaccg atgagtccgt
     7861 tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa aagagggaaa gaagttgtga
     7921 cagaagtgag cagcttcaag gagctgcgac aagacttttg tcacagctcc ttttttctat
     7981 gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg ctattttatg
     8041 ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta tttatctgga
     8101 tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg caatcatgca
     8161 agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc atgaaaaatt
     8221 ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg agatcatctt
     8281 taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt tctcgcgtca
     8341 aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc ttaataccat
     8401 gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg aaaaagggaa
     8461 tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg tctctgtgat
     8521 gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg aacttttagc
     8581 ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc aaacgatcca
     8641 tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca atgggccaaa
     8701 aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc ttttaggcgg
     8761 ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct ggggtatggc
     8821 aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca aatataaagg
     8881 attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc aagtaaatgg
     8941 tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg aagcaaatga
     9001 tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt cagcttgtac
     9061 agcaggaaat atcgagcctt cacacgtatt agaagcaatg tatggagaag atacaccgat
     9121 actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg acatccagta
     9181 tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat tcttatatga
     9241 acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa cctagtaaaa
     9301 atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa atcatggcat
     9361 ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat cctgatgcaa
     9421 aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat tatcaattga
     9481 tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa atcactgtat
     9541 caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg aaagcagtca
     9601 atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt ctgatggatg
     9661 ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata aaaagacaac
     9721 tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc aaattagttg
     9781 tcttttttat ttttaaattt aacacat
//
LOCUS       DAYYAG010000102         9794 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000102 DAYYAG010000000
VERSION     DAYYAG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.104"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..198)
                     /locus_tag="ACHARQ_002199"
     CDS             complement(<1..198)
                     /locus_tag="ACHARQ_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410598.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYF"
     gene            complement(214..828)
                     /locus_tag="ACHARQ_002200"
     CDS             complement(214..828)
                     /locus_tag="ACHARQ_002200"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8410599.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(975..1658)
                     /locus_tag="ACHARQ_002201"
     CDS             complement(975..1658)
                     /locus_tag="ACHARQ_002201"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8410600.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1767..2225)
                     /locus_tag="ACHARQ_002202"
     CDS             complement(1767..2225)
                     /locus_tag="ACHARQ_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8410601.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2309..4072)
                     /locus_tag="ACHARQ_002203"
     CDS             complement(2309..4072)
                     /locus_tag="ACHARQ_002203"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8410602.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4293..5753)
                     /locus_tag="ACHARQ_002204"
     CDS             complement(4293..5753)
                     /locus_tag="ACHARQ_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410603.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5961..6887)
                     /gene="glsA"
                     /locus_tag="ACHARQ_002205"
     CDS             complement(5961..6887)
                     /gene="glsA"
                     /locus_tag="ACHARQ_002205"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8410604.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6910..8337)
                     /locus_tag="ACHARQ_002206"
     CDS             complement(6910..8337)
                     /locus_tag="ACHARQ_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8410605.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8368..9582)
                     /locus_tag="ACHARQ_002207"
     CDS             complement(8368..9582)
                     /locus_tag="ACHARQ_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8410606.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2903 a         2040 c         1624 g         3227 t
ORIGIN      
        1 aaagtaatta aacaatccgc taatcgtttg atctggcatt tctatttcct taccatctaa
       61 agtaacagta gtaacgacaa aatggtcttg caatggcgga acttctaagt actgcccttg
      121 cagaaaatca ttcactgttt catattcttt tttcatttct tttgaatctg tcgatggttt
      181 atagcttatt tttaacataa ctagtgctcc tttttattcg ggacgtgcaa tcgtgaaaat
      241 ttctcctatt gctgcataat catttcctgc caaacggctt acagcattca attttcttgg
      301 atcgatcctt cctgtcttat catcatagac tgtttcattt aaatgatatt tctgtacttc
      361 tagcaaaaat aaatcagctg ttactttatt tttattccgt atctcgacat gttcatataa
      421 ggatgtttca aaacgaactt ttgcttctgt caatgctggt actgaaatga ttactgaatc
      481 agttaaagtc aagtcagtca ggtacagttc gctttcctca ttaggcagat tggctgctgt
      541 tttatttact tcttctacgt tgtcctcatc aaggatatgg ataacagctt ctcttgtctc
      601 aagaatattc tttgctgtgt cttttactgc atcttgtttt ctctggatcg ccaaggataa
      661 gatgggtcga ttcgaactga cgatattgaa gtaactgaag ggtgcgatat tcactatatc
      721 ttttgctgat ttagtcgtta ctactgctac tggtctaggg atgactgaac caatcagcaa
      781 cttgtaattt tcacgttctg tcaattctga aggactccgt tcaaacatgg tttcgccttc
      841 tttctataaa attcgttaaa aagcagatct tgaagacaaa atgaaaagta atgctttttt
      901 catcttctta gaatctgctt taaactatct atcaattttt cttttttgtc aagaatctgt
      961 gccgtgcgtg ttttttagtt atttgcttta cttgcggctt cgacatatct tttagtgatc
     1021 aattgcggac gggtgataat agatccaact acgactgaaa aagcacctaa ttcatatgct
     1081 ttctctactt gttcaggaga gtcagtatgt ccttccataa ttactgggat atcgacttgt
     1141 tctatgattt gattcaataa atcaaaatca ggtgtctgcg tctttcgtga atattctgta
     1201 tagcctgcta aagtcgtaga caaaagatca aaaccatctt cttgcgcttg aagcgcttcc
     1261 tcaaaagtcg aaatatctgc catagctaac cgaccgtgtt cgtgaatcat ttggacaagt
     1321 tcagcagttg ttttttctgt cggacgttta cgagaagttg catccagtgc aatgatttct
     1381 gcttgcgtct ccaaaagttc ttccacttct ttttctgtcg cagtaataaa cacttctgac
     1441 ccatcgtaat ctcttttgat aatccctatt accggtaaat caacgattcg cttgatttca
     1501 ttgatatctt ctgtcgtatt tgctcgaata ccaactgctc cgccttcttt tgcagccaaa
     1561 gccattctcc ccataataaa agaactgtgt agcggctcgt tttccaaagc ttgacaagaa
     1621 acgacgactc cgcctttgat ttgttccagt gtatccacaa tatctcctcc agtaatgtta
     1681 tataaaaata cagcttcata taaaaatgta acccttttct attcaggaga caataatgag
     1741 ctatttcttt cagcagacct ttcttactaa aagaaaatta cttcaaattc ttcgtatacg
     1801 accagcttct cttctgtaaa ttctatgttc aacgcttctt tataagcatg tttaaaaaac
     1861 tcagcatgga ctttccgcca ataagctaat gtccgatccc cttctccttc tttccaagca
     1921 tgctctttcg tgacttcact aaaaggcaag atggtcacat ttatattttt gatgatacaa
     1981 actggttcat tttttccatt caggaccacg tctaattgac caacttttgg taaaggctca
     2041 ttctcttgtt tatacagatc gtaaccactt gatgtaccag tctttatctt ttttacaact
     2101 agttcgctca actcgtctgc cattttttct gtatccccaa atgaaaagaa acctaatttt
     2161 tctgattgat aactaggata tttttcttgg aactttttga catattcttc tacttgctga
     2221 tacataacat ccaccccttt gctttttata atgaaaaaac catccagata ctagtataaa
     2281 caattatctg gatggtgagc ataatgaatt attgaatatt caagacacgc agactatctt
     2341 caacgatttg ttttggtgtt aaatgataac gttcataaag ctgccctaca ggaacgctat
     2401 ccgtgaattc tttctctgca ccaaaattta atacgttcat tgaagaatca ccataataac
     2461 gactgatatt ttcgccaaat cctcctgaca aactaccatc ttctaatgtg atgactaatt
     2521 cgtgtgtttc ttttaattta tctaatgttt ctacgtcatt ttctgtgatc gttcctggat
     2581 tgatcaacgt tgcttcgatt cctttttttc taaaagcttt tactgtttct tttcctaatt
     2641 caagaaaacc gcctaaagct aaaattgcta cttttcctcc attatttact gtttgatagg
     2701 ctggaatact ataatcattt gcgatagctg taccatgttc tacaccatgt gtaggaattc
     2761 ggattgctac aggctgcgct gtttgttcga acgcccaatc tagcatagag atcatatcct
     2821 cttttgttgc tggagcaaga tatttgatgt tcgggatact tgataagtaa ggaatgtcaa
     2881 atgttccttg gtgtgttcgg tcactagctg agatcgttcc gccaccgact aaaataatca
     2941 ctggattctc gttgatcgct aaatcatgtg ataattgatc atatgcccgt tgcaagaaag
     3001 tacttgattg gaagacgact ggacgtactc cctgagaagc caaagcagaa gcgaaagtga
     3061 tagagtgctg ttcagcgatc cccacgtcaa aataatgatc tggatgtttt tcttggaatg
     3121 ttttcagtcc aaaggcgcct ggtatcgctg cattgactgc ggcaatagga gctgtacttg
     3181 tttgcatttt cttatccaaa taatccatga tgatcatatt aaatgtttct ccagaagtta
     3241 cttgctttgc ctccccggtg gacagatcaa atggtacatg ccaatgatat ttcattttat
     3301 tatcgattgc cggctgataa ccgcgacctt tttccgtatg gacgtgaagt acaatcggat
     3361 gatcaatatc ttttacttct tcaaataaac ggatcaccgt ttccagatcg tttccattat
     3421 ccacgtaacg ataatcaaat cccattgctt taaataggtt attgctgctt ttaccgttgg
     3481 tttgacgaag ctcagctaag tttttatata aactgccatg attctcatca atagacatct
     3541 cattatcgtt tactaatacg attagattgg attgtaaaac accaccattg ttcaatcctt
     3601 caaaagctaa gccaccactt agggagccgt caccgataaa tgctaccaca tttcctgatt
     3661 ctttcttcag atctctcgct ttagctaaac ctgttgctaa gctgatagat gtagatgtgt
     3721 gccccactgt aaaatagtca tgtttacttt catgttggtt ggaatatccg ctcacttcac
     3781 ggtaatgtcc tttttcaaaa ccattttttc gaccagttaa aagcttatga ccataagact
     3841 gatgggaaac atcccaaacg attttatcct ttggtgaatc aaatacataa tgaaacgcaa
     3901 tcgttaattc tactacacct agattcggac caacgtgccc acctacatcg ctgtcctttt
     3961 gcaagatttt ttggcgcatc tcttctgcca actgtttcat ttcctcaaca gataattttt
     4021 tgacatctgc ggggctattg attgcatcca atacatgtaa tactgtactc ataatacatt
     4081 ctctcctccg tactatcttc ttttaatgaa aatagatttg ttacacttta tctattctac
     4141 tacttagagt tgactctaag tcaatagcat tttaaaaaaa tttcagagaa agcttgttat
     4201 ataagtagat cttcgcacga atcaggactc aaataaaaac cttctacaga caattaaaaa
     4261 gctttgtaga aggtctccta attaaggtct catcaagaaa tatgtttttt taataggtga
     4321 agttcctgag caatatgata caaatggatg tttgcacctt ttaatgagat tggatagatc
     4381 tttgtctttt cagtttctaa tccattttga tagctagcag catcgcacaa aaccactctt
     4441 tttttcgcac atagacattc atcaggcaat tcttctaatg aacgaaataa aataggattt
     4501 atttcagcga tgccttcttc cgcatacaaa tgaaattggg ttcgataaaa aaattgtttg
     4561 actgaacttt ctgaaacaac aaaaatttct atttcctgtc tccccgcttg ttcactgttg
     4621 atcaaagaaa gacttttgac gaattttcga atgaaatttt cattccatct tagttggatt
     4681 tgatttttct ttttccactc ttccaatatt tctacaattt ttccgtacag aagttgttct
     4741 ttttctgata gaagatagat cctttccggt aaaaatactt gtccatctgc ccacaacgta
     4801 cggacaaatt gtaaaaagga ttgcttgaaa ataaaatttt ctgcatcgtc gatttcagcg
     4861 atactaataa tctgtaaaat caattcattc agtgataggt cattgagaac aaaattttta
     4921 taaacaactt cgaaatcttt tcttaacagc tccatgttct cattggtata atttctactg
     4981 ttgaacaaag aaaaaatata atagacatct tcttcttgta agtctagaat tcctttttgc
     5041 tcttccaatc cttgacagac taattgaaat aacggcttcg tttctgcgct tttcttatct
     5101 tcttctttca gtccaagggg aaatgccttt cttccgattg cgatctctat tcctcgtatg
     5161 ataaaggact tttcatcttc cgaatatctt cttttaaaaa aatgagtctc tacataatca
     5221 atcaactcgt tacaagcttc ttctacatac ggatttttat tttcatagcc ttcccaattg
     5281 acataacgta ctaaagctag caagatgttc cgcgagtcaa attctgggat aatgatttct
     5341 ttatttttca aataatcatt ttctttaaaa aaatccaata cgattttttt gattgtatat
     5401 actttgctca aagaaataaa ttcaagatca gccagttctg tactcgtgaa tataccatta
     5461 aaataataac ttaaaaactt taaaaaataa gattgaccgt acagaaactg tgtgagctca
     5521 gaaagagcaa taccatgttt gaatacgagt ttatacttat gcttctctct tacttgttct
     5581 tttatacaat attctagaca ctcggctaga ctttctagat cgtgaaggat cgttggtgta
     5641 gtcaccctta gagaaatagc tgtttgttcg atgtctattt cttggaattc aaataattgt
     5701 tcacacagat acacttttct aaatatattt cgctcgatgt aattttccag cattcctgat
     5761 acacgcctcc agtttttctt atgattttat ttaatcatat ctgtaaatcg tatttttgtt
     5821 tcctttttat ttagaaaagc cctttcaaaa accaataaaa aaagaaactg ggacaaaagt
     5881 caaacaagct cacaatttca gcctcattgt aggtagcttt gagccgtttg gacttttacc
     5941 ccagcctctt tcaagaatgg ttattcaaag atgtcagcgt ctagttcttt gactacatct
     6001 ttcaacaatt ggataccagc tgcgctgtta ccaaatggat ctagtgccgg gctgaagaca
     6061 cctatgccgt aacgatttgg cacagctgcc atcaacccgc cgcctacacc gctttttgca
     6121 ggtacaccaa catggacgga aaactctcct gattcatcat acaaaccagc agttgtcata
     6181 attgatttga cgattgtagc actttcttca gtaatgattt gtttgccatt ccaaggagca
     6241 attcctttat ttgccagcac agcagctaat tttgctatgc ctgtagcggt gacattgagg
     6301 gagcattgtc tgaaataagc atcgagtatc tccggtacgt ctccttcgat catttgatta
     6361 cctttcatat agtaagcaag agagcgattg atatcacctg ttcttgattc agaatgatag
     6421 atttcttcat tcaatgtgat attaggatca tcacagatct ctttcataaa tgacaagatt
     6481 cgctcaaatt tttcatctgc atctttaccg gcaatcaacg aggttgttgc aatcgctcct
     6541 gcattaacaa aaggattcat tggatgtttg cgatgattga tttccatatt catgatagaa
     6601 ttgaaggcaa aaccggttgg ttccgtgttg attgtttgaa agactttttc tattccgttg
     6661 tctttgattg ctaataacaa tacgggaact tttgacatgc tttcgatggc aaagcgcaca
     6721 tcggcgtctc ctccctcaat cagttgtttt gtatccaaat cataaatcgc catacctaat
     6781 tgtttgggat ccacttcagc taatgctgga atgtaatccg ccacttgtcc tgtttgataa
     6841 tagtggaaat tttttccaat caattgttct aattctttta cttccatttg aataaccagc
     6901 tttcattttt tatttttctg catgctggaa tttatgacgg catgcgtaga tcgtaaatgg
     6961 aataactaag acgataagat aagaaacaat cagaatcgtt tcataaatcc catgctgtcc
     7021 agctgcgata ctagcaggtg gaacaaatga gatacaaaaa gcaaaaagtg aagtcaaaag
     7081 acctaaacaa gcaacgatta cttttacaac ttttccgcct ggaacctgat aggctctttc
     7141 aagactgtca tctttaaaaa ccagcttcaa atagcccgcg aaaaacataa agtacaacat
     7201 caggtagatt acaacagtca atgacatcgc tgcaagaaat gaaagattat tccccccgcc
     7261 gcttaatgta aagacggctg cccagattgt tgcaacgatc ccatttgcga tcatcaaatg
     7321 agcaggaaca ccgtgtgtgt tttcttttgc aaaatattca ggaagtaatc cttcttctgc
     7381 tgctacgtga agtgcttttg aaggagaggt tacccaagaa gcaatttctc cgatgacacc
     7441 taaacaaaca agaatcgcaa gaattttcgc aatccatgca agatttggat caatgtgtct
     7501 taataggaat tctaacgatt gaatcacccc tgtattaaga gacaaaccat gttgaggtac
     7561 tgtagtagcg acggtcaatc cgccaaaagt atctaaaatg attgccagaa taaccaatac
     7621 aaacattgct aatggataat cctttttagg gttttccatt tcatttgcat gactggcaga
     7681 tgtttctacg cccatatatg ctaaaataaa ggaaacaaaa acaaccagcg taggcaattt
     7741 cgaaaaatca ggaatcaatg ttttcataga cagtatcgtt tgtagtggag cgcctgaagc
     7801 gacataatgg atccctaagc caaacaagac taatgctggc aacaagatac cagctacaaa
     7861 accgatccga gcaatcaatg aagtgtattt ggttcctttt aactgtgaga gtaaaatagc
     7921 ccagaaaata atcaatagga ttgccaattt cagatattta ttgttttgta tagcaccaat
     7981 acccgttgca taggataatg caccagttaa gaaatacagc atggtcaagt agccaactgt
     8041 tatctcaaac cattggaaaa agattgcggc aaaaccgaat cgttttccca atgtgcgact
     8101 gacccaagta tagacaccgc ctttttccca tcctttcacc gttgccatct ctgctgcgca
     8161 caatgccaca gggataaacc atagcaaacc gcctaataac aagaaaaata ctaagctgaa
     8221 gcctgaggtc gcgaatgtgg gatattcata taatgaaacg accattgaga tggtcatagc
     8281 caaaaagcca aagaacgtta atttttttgc aggaattttc gttttgcttt gttgcatgaa
     8341 tcttcctctt ttctatttga atttgtttca attgagtttg ttgccaaaga agaaaaagtc
     8401 caatccttct tcttgttctt cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt
     8461 taaaatcact gttgtcagca cataggcata cacggcagta atgatgacac caatcacttg
     8521 aatccctaag aagtaccagc tgcttggatt cgttagtaaa tggctgttag atgcaaagat
     8581 ccctatcaga atcgtacctg tgaatccgcc catgccatga acaccccaca catcaagtgc
     8641 atcatcccat tgtttctttt tacgaaaatc aacagcagca tgacagacga tacccgcaat
     8701 gatcccaatc agcatggcac tttttgcttc tacataaccg gcacaaggcg taatggctgc
     8761 caatccagca acggatcctg ttagtacgtc gacaaaatca aagccatttc cttttacttt
     8821 tgcaatgatc agccaagtaa tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc
     8881 attgactgct tgttcatctg ctcgaagtgc accaccggaa ttaaaaccga accagccaaa
     8941 ccaaagcagt cctgtaccga tagcagctgc cataagactg ctatgctgac ttctttctcc
     9001 taaaggaatc tttctttgac caagtgtgag gatacatgct aaagagccga atccagctgt
     9061 cgtgtggatg actgctccac cagcgaaatc tctaaatccc atctgatcca aaaatccgcc
     9121 accccatatc cagtggcaaa cagggaaata aatcagcaga ttccaaaaaa taacaagtat
     9181 aatatagcct tttaaattca atctccctgc aaaagctcct gtcatcaaag gaacggtaat
     9241 cacgataaac atcagttggt aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa
     9301 catgttgggg aagcttgtaa catgtctcat caaaaagaaa tctgccgggt ttccaataac
     9361 accgccgata tcccgtccaa aggccaatcc gaatccacca aatacccaca taatcgttgt
     9421 tatcccaacg gagagaaaag attgagacat gatcgttagt atattattct tccgtgctaa
     9481 tccgccgtaa aaaaaagcta gaccaggagt catcaggcaa accattgctg tacataaaat
     9541 catgaatgct gtatctcctg tattgatagg taatgtattc atttttttcg cctcctttct
     9601 acgattacga gtataaaaga aggtgtagtt gtttttttct tctgtttttt tgaattaata
     9661 cttgcattta taaaagacag acaattaata aaagcttgat gtgttgtgct ctttttcgtt
     9721 tttcacacaa ataaagacaa aaaataaacg gtcttctttt tatagaaaac cgtttatttt
     9781 cgtctttttt tatt
//
LOCUS       DAYYAG010000103         9732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000103 DAYYAG010000000
VERSION     DAYYAG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.105"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHARQ_002208"
     CDS             complement(<1..244)
                     /locus_tag="ACHARQ_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8410607.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACHARQ_002209"
     CDS             complement(237..1829)
                     /locus_tag="ACHARQ_002209"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8410608.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="ACHARQ_002210"
     CDS             complement(2339..3064)
                     /locus_tag="ACHARQ_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410609.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="ACHARQ_002211"
     CDS             complement(3218..3544)
                     /locus_tag="ACHARQ_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410610.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="ACHARQ_002212"
     CDS             complement(3581..3946)
                     /locus_tag="ACHARQ_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8410611.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="ACHARQ_002213"
     CDS             complement(3983..4198)
                     /locus_tag="ACHARQ_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8410612.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="ACHARQ_002214"
     CDS             4327..4662
                     /locus_tag="ACHARQ_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8410613.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="ACHARQ_002215"
     CDS             complement(4738..6735)
                     /locus_tag="ACHARQ_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410614.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="ACHARQ_002216"
     CDS             complement(6833..7108)
                     /locus_tag="ACHARQ_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410615.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="ACHARQ_002217"
     CDS             7340..8074
                     /locus_tag="ACHARQ_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8410616.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="ACHARQ_002218"
     CDS             8113..9255
                     /locus_tag="ACHARQ_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8410617.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3190 a         1816 c         1423 g         3303 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     9361 tgccttccaa tgactctttt gggaatcaga ggaaagaatc aggctttata gagatatttc
     9421 atctaacaaa ttactttaga ctgacagtat atatcagcag gtactgtcat cgggaacgta
     9481 gccaatatcg tatccaaaac agcaggtaga gaagctattt gaggattcaa gttattctat
     9541 ttcaaccatg aagttaagtt cattaaaaat aaagatagtt ggtatttcaa taatttttgt
     9601 atagagttaa tcactttatt gatgctaatt catcttgatt ggtattataa tcgtattcaa
     9661 gttgcgtggc gtgcttttgc tagaaaatat ataactgttc tttataaaga aatataagga
     9721 ttaaacattt tt
//
LOCUS       DAYYAG010000104         9567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000104 DAYYAG010000000
VERSION     DAYYAG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.106"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..1023
                     /locus_tag="ACHARQ_002219"
     CDS             154..1023
                     /locus_tag="ACHARQ_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8410618.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1232..2257
                     /locus_tag="ACHARQ_002220"
     CDS             1232..2257
                     /locus_tag="ACHARQ_002220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8410619.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2296..3246
                     /gene="manA"
                     /locus_tag="ACHARQ_002221"
     CDS             2296..3246
                     /gene="manA"
                     /locus_tag="ACHARQ_002221"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8410620.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3340..5043
                     /locus_tag="ACHARQ_002222"
     CDS             3340..5043
                     /locus_tag="ACHARQ_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8410621.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5045..7078
                     /locus_tag="ACHARQ_002223"
     CDS             5045..7078
                     /locus_tag="ACHARQ_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8410622.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7089..7529
                     /locus_tag="ACHARQ_002224"
     CDS             7089..7529
                     /locus_tag="ACHARQ_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8410623.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7729..8856)
                     /locus_tag="ACHARQ_002225"
     CDS             complement(7729..8856)
                     /locus_tag="ACHARQ_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8410624.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8859..9536)
                     /locus_tag="ACHARQ_002226"
     CDS             complement(8859..9536)
                     /locus_tag="ACHARQ_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8410625.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
BASE COUNT         3250 a         1657 c         1952 g         2708 t
ORIGIN      
        1 acctcttttt ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt
       61 tctcacattt tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta
      121 aaataaagct tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc
      181 acctatttaa aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac
      241 aagaacgttg ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca
      301 gaagaagctg gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg
      361 ggggtgatca aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca
      421 gatatccttt gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat
      481 ggacgtatgg ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa
      541 gagaataaga acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga
      601 aaatctgaag aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta
      661 aatgttacta acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta
      721 cataatgaag aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca
      781 gaagctagcg gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg
      841 aaattcatga tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca
      901 gttgatttct tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc
      961 gaatggtggt tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct
     1021 taactttttc tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat
     1081 cgcttattct agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg
     1141 cgcagttttt ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg
     1201 taagaatctg atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt
     1261 gaaaaagatg gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca
     1321 agtagaaaca tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga
     1381 agatctgttg tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt
     1441 acgtctatta gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga
     1501 atttgaaaat cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac
     1561 atggatacca aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct
     1621 agaaccaggt gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct
     1681 tgggttgatg ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt
     1741 attggaacca ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt
     1801 tcccacagaa caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc
     1861 aagagttttg gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat
     1921 tacttttgtc caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga
     1981 agagacgatt gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt
     2041 aggatgggac ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta
     2101 tacaggtacg tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa
     2161 tcgcgtacat cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga
     2221 aaactttttg aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg
     2281 agaggagttt gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt
     2341 tggggaggaa gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga
     2401 gaagattggg cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc
     2461 aaagggaaaa gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca
     2521 gaaccagtgt ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa
     2581 gtacatccag atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc
     2641 tggtatatca tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca
     2701 agagaagaac tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta
     2761 ccagttaaaa aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa
     2821 ggaatcatga ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac
     2881 gacagaaaag atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta
     2941 acaactgtcc ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca
     3001 tcgatcgtca cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga
     3061 atcacatctt tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga
     3121 gaccttgtcg ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca
     3181 aatggcgttt ctgagtgggc ggtccaaggt gaactagcaa tcattgcgtc tgaaccaggc
     3241 aaatagttcc cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat
     3301 aaaatccgtc catcaataaa tagaagtctg gtgaaaaaga tggaatacaa atggaaagca
     3361 aacaacagca gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa
     3421 atgatcatgc caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa
     3481 ggtgtgtctg gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg
     3541 cttttaccca tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca
     3601 gtcgccggtg caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta
     3661 ggagcgatga ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc
     3721 catgtgaaat tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact
     3781 ggtagcgtta tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac
     3841 ttgttgctcg cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt
     3901 ctgatcttag aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg
     3961 acacctttag gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag
     4021 gcaaatcctg gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag
     4081 gaaaaagtaa atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc
     4141 tattttccat ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca
     4201 agcggcacgt tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg
     4261 tcactactcg tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg
     4321 atagcaatca gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat
     4381 aaagaaaaag agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt
     4441 cattccgtca tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg
     4501 ctgaaaaaga aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat
     4561 gaattgaata accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca
     4621 agagaaaaag tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc
     4681 ttgctgtcac gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata
     4741 gcagatatca cagaacaaaa aacgtcgcct gctaatattg tatttttgta taaaaacaac
     4801 agacgcggct cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa
     4861 aaacagtttg ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt
     4921 tatatcgcac aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta
     4981 gttctagttg aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata
     5041 taagatgtat ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc
     5101 acctgtatct atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg
     5161 cgaattagat aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc
     5221 tagaggctca tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac
     5281 agatgggaaa agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt
     5341 gttactgacg acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa
     5401 taccactttt tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga
     5461 aatcattcgg aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac
     5521 agcgaatgta ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga
     5581 tgcatcactg aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa
     5641 agtaatcggg gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca
     5701 tttggttttg atgctgacat tgacgatgga gcgtgtgacg aaaaatcatt tactgacaaa
     5761 tgagacatat aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc
     5821 acggatcgct tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg
     5881 attattaaat gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac
     5941 acagcttgct tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca
     6001 tttttatgaa gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc
     6061 aagagccgtt ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca
     6121 atacgaggaa gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa
     6181 attatccgaa gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag
     6241 tccgaaagaa agcaatatca gtattgcagg attttcgcca agtggattag cttctactag
     6301 catgttggaa ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg
     6361 gattgctgat ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc
     6421 tattttacca ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga
     6481 agtcaagcag ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac
     6541 gtataacagc aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat
     6601 ggatcaaatc aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac
     6661 gatttccgaa acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga
     6721 agaggtggtt catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca
     6781 tacaaatatg gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat
     6841 tttcaatttg aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc
     6901 ccgtatgtta gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg
     6961 aaagatcagt ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa
     7021 taaagaaatc gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag
     7081 gtgaaaagat gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt
     7141 ggaaagaagc gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg
     7201 catatatcca atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg
     7261 tagccttgcc acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag
     7321 tccaagtgcc tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct
     7381 tatttgtagt ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt
     7441 tctgttcaga catcgaccaa gtaatggcgc tcagtgatgc ggaaaccatt gaagaagtcc
     7501 aaatgatttt tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc
     7561 atcaagtaat aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct
     7621 tattactgcg gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat
     7681 aggggacggt gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt
     7741 ttttatatag atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt
     7801 caagatacca ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc
     7861 tatgatcaca ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat
     7921 ccatccaatc gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa
     7981 aagattgact agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa
     8041 gagcacccac aacaacagga acagaaagct ctggaaaaga tactcataca tcggcaggat
     8101 tcgtatctcc tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt
     8161 gattattact agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc
     8221 ataataaatg gtaaaacgga tgctatgttt ctttaccata ctatccagta atagattgac
     8281 tagagagatt acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt
     8341 tatacataaa atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc
     8401 taaagagaga tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat
     8461 ataacggagc aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc
     8521 agaaatcagt cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg
     8581 gacattccct gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa
     8641 actttccaca aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat
     8701 ctcttcatag ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa
     8761 aagtgggaat gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt
     8821 tagtaagaga ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga
     8881 taaatagcta aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga
     8941 aaacatgctt tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc
     9001 gtcttcccct ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg
     9061 tctaatccgt tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca
     9121 gcaagcaaga catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac
     9181 tcagtcatct tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat
     9241 ttgatcaaag tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct
     9301 agaaaaaaga aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac
     9361 aattgatcat aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca
     9421 aatccgttcg gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt
     9481 cgaatcacac gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat
     9541 aggcctccaa tataaaattt aataaca
//
LOCUS       DAYYAG010000105         9457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000105 DAYYAG010000000
VERSION     DAYYAG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.107"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACHARQ_002227"
     CDS             32..388
                     /locus_tag="ACHARQ_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFM8410626.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACHARQ_002228"
     CDS             404..754
                     /locus_tag="ACHARQ_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFM8410627.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACHARQ_002229"
     CDS             783..1562
                     /locus_tag="ACHARQ_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFM8410628.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACHARQ_002230"
     CDS             1573..2235
                     /locus_tag="ACHARQ_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFM8410629.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACHARQ_002231"
     CDS             2254..3360
                     /locus_tag="ACHARQ_002231"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFM8410630.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="ACHARQ_002232"
     CDS             3350..4441
                     /locus_tag="ACHARQ_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFM8410631.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="ACHARQ_002233"
     CDS             4454..5194
                     /locus_tag="ACHARQ_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFM8410632.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="ACHARQ_002234"
     CDS             5482..7422
                     /locus_tag="ACHARQ_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8410633.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..9431
                     /locus_tag="ACHARQ_002235"
     CDS             7509..9431
                     /locus_tag="ACHARQ_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8410634.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         3110 a         1573 c         2120 g         2654 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     9421 gttatgaata agtagcgatt aaaaaactgc ctttata
//
LOCUS       DAYYAG010000106         9453 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000106 DAYYAG010000000
VERSION     DAYYAG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.108"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1510
                     /locus_tag="ACHARQ_002236"
     CDS             275..1510
                     /locus_tag="ACHARQ_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8410635.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2509..2769)
                     /locus_tag="ACHARQ_002237"
     CDS             complement(2509..2769)
                     /locus_tag="ACHARQ_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8410636.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2796..4097)
                     /locus_tag="ACHARQ_002238"
     CDS             complement(2796..4097)
                     /locus_tag="ACHARQ_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8410637.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4389..4847)
                     /locus_tag="ACHARQ_002239"
     CDS             complement(4389..4847)
                     /locus_tag="ACHARQ_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8410638.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4860..6191)
                     /locus_tag="ACHARQ_002240"
     CDS             complement(4860..6191)
                     /locus_tag="ACHARQ_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8410639.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6202..7644)
                     /locus_tag="ACHARQ_002241"
     CDS             complement(6202..7644)
                     /locus_tag="ACHARQ_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8410640.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7631..8395)
                     /locus_tag="ACHARQ_002242"
     CDS             complement(7631..8395)
                     /locus_tag="ACHARQ_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8410641.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8499..9374)
                     /locus_tag="ACHARQ_002243"
     CDS             complement(8499..9374)
                     /locus_tag="ACHARQ_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8410642.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         3052 a         1970 c         1431 g         3000 t
ORIGIN      
        1 tataattatt ttattttttt atgttctcat gtttattttt aatgacacaa cttctcgtat
       61 ttatctggca caacatataa atcgatcaaa aaattgttgc gtgttttttt gaaaattgga
      121 gtttcaactt gattacaaaa tttaaattta gaagttgtga aaacagcgag agacagttta
      181 atattcaaaa tatacatagg ctactcgaac gctgtgtttt ttagtatact agtaagaaac
      241 gaagggatag actcattcca ataaggagga tttcatgaag acaattcttt ttgctcctgc
      301 agtattcaat ttagctgaga caacacgaat gattgaggtt gccaaagcta tgaaagaccg
      361 ttattactgt gtattctttg gcttttctaa aacttttgct cctttgatca cagaaagtgg
      421 ctttgagtat catttactag cgccgatcct tacgaaaaaa caagaagaac aaatcataaa
      481 atttgaccag ttgcgtacac ttaaaaaccc ttttaccaaa gaaatggttg cctctcgtat
      541 cactagtgaa cagagtctga ttgctaagtt tgagcctcga ctgattgtga caggttcgaa
      601 tgtgacaatt tttttatcgg cccgcaataa aaagattcct ttagtatatg ttaaacccta
      661 tgctttgagt gaaccttttt ttcaatcaga gcatgcgctg atgccccacg agttacgttt
      721 cttcggccct ttaagcaatc ccctttggca ctggacaaaa aagcgtatag taaatacccg
      781 atggatcccc aatgccttca aacacgctgc acaaaaagaa atgcttcctt taccaaaaac
      841 aagtttacaa atgatggatg ctgatctaaa tctcatcaca acgcctaaac tatttttgga
      901 agaagaacag ctgccggaaa attatcgaat cgttggtcca atttttgcta agctcagtac
      961 ttccttgcct gaagaagtgc taacttttat acagcgaaag caacaagaaa gaaaacgtat
     1021 tatctatttc gcaatgggta gttcaggcaa tccgcgtatg ttacgcaaaa ttttgttctt
     1081 cttacaaaaa caaccggatc tagcggttat tgcacctatt gactatctgc ttgatgacat
     1141 tcaatctgac tcagaagatc ttttaataac agaatatctg tctagtcatc ttttgcatga
     1201 attcattgat ttttctttta tccacggcgg ggaagggacc gtccaaactg cctgtgaatc
     1261 cggcaaaccg tttatcggtg tggggatgca atttgagcaa agctgtaata ttgactattg
     1321 tgtaaaatac ggaaacgcaa tcgccctcac aaaaccagtg actgaaagaa aactgcagat
     1381 tgctctgcgc gaagtttctt cacccaagat tcgtcagcag gcacgcacgc tgcaagccgt
     1441 ttttccaata aatggtccac aacaagcaat tcaagaaatc gaaaccttcc tgagcaacca
     1501 cccaatatag tttcaagtag aaaaagcttt tttaatagag actcatcagt aatttcgcta
     1561 ttcgagaccc agacagtgaa aatacaaaaa aagtaaatag tggattgaaa agttagagtt
     1621 acttaaatta cggtgtctaa aaaacacaaa atcatcctta aaataaaaga tctgacgcta
     1681 tccaaatgag cataggtagc gtctgccatt tattcttagt caaattcttt gacacctttc
     1741 tctggttgga tcatctcaac gtttcttcaa aagaaagacc ataataatta ggcaaataca
     1801 ttttcttaaa atgtgtcata tagatctaga agcatcgctt tgtagagatt tgtgccgcta
     1861 gtcccctcaa caattactaa atcaaatgta ggatttacca ctttatcttt gataatcaac
     1921 ctccctcttc tgttgagaca atgatctcgc aatccgatgc atactcctat ataaactgcc
     1981 agcaatcccc acatattgag tagatcatgt cagaatgttc tatgtcgtca gcaacgatac
     2041 actaaaagtc acgttcacca tgactgattg ctattcccag cacaattatt tctatacacg
     2101 tttcatgatt tgaataaaca ctcacatcat acatattacc attcatacac catactgcag
     2161 cccctactat atggttgtaa gctcccaaat ctatatttga cttgatcact ttggtagtag
     2221 atctactaat tctaaatcca ttgctgataa aatttccatt ttttcctcct tctttctgcc
     2281 aaaacagtta aaacaataat aatctttatg taaaaaaggg ctgtgacaaa agccttgtca
     2341 cagtcccata ttccgaataa acggtggtca aaagcttgca tctgcggtaa taagttcaac
     2401 caaacgaaaa ataagccaac atttcacaaa aatttaagaa gcaattttcg taaatttctt
     2461 cttatttctt gaagctaacc acttctgtta caaccacttt tagaacattt aaagcttcga
     2521 tgtgacaaac catctatgct gctcgatcat aaaattgata gcaaaaattc cacaaagtac
     2581 atatgtcccc catgaaaaca aaacattcat atgcggtaca atgatctttg gaagaatcac
     2641 gatcaaaaat gatgccagtg cccaaatcca tcgattgatt tgaatatgat atgtagctag
     2701 catattcaat ataaggtgac tagccgttaa taaaaagata aaaacaaaaa ataaaatcag
     2761 ccaatacatt gtttcccacc ttctaaaaac caaacttaga cagtctggac ttcgttttct
     2821 ccacgacttt gttcatctga cagcatcttt ttatcataag cttttacaaa tggataatag
     2881 ataataaatg ctatcaatgt gttgatcatg atcaagataa caacacgcca gtcatttcct
     2941 gctgaaagat aaccgccgat tggagctggt aatgtccatg ttggcaaaac agtaaatcta
     3001 ttgaccattt ctaatttgac tgcaaaataa ctgataatag tcaaaactaa aggaactaag
     3061 ttgaacggga tgaacatgta aggattcaac atcataggag ctccaaaaat tatgggctca
     3121 ttgatattga agataccagg aataatagaa aaacgaccaa cctctttcaa gaattttgat
     3181 ttacagaaga atataaacaa aacagatacc acgatcgttg ctcctgcacc accaattgtt
     3241 accgtccatt gataaaactg ttcaggagcg atatagggta attgggtggc agctgtacca
     3301 tctgccaatg cctttgcatt ctcatccaac atgatcagcc acattggacg aacaacagcc
     3361 cctacaatac tcatgccatg gataccaaat gaccacaaca aatggatcaa aaacattggt
     3421 agtaaaacac caagcaaatt attccctaaa atattggtta aaggctcgaa taacgataat
     3481 aaggcagcgt ttacatcaaa gcccgcgacg cttcgaatca gccaagtaat tgttaaaatg
     3541 actgcacctg gaatcaatgc ctcaaaacta cgggcaacag caggtggcac ttgttctggc
     3601 attttgatag taatatttcg atttttaaag aaacgatata cttccactgc aaaaatcata
     3661 gataagatcc ccgcaaacat cccagatgct cccaaagaac tcataggtaa gacccagcca
     3721 agaggtgcct cttcaggagt taaaacgttt acaggaactt gtaacataaa gaacgttcct
     3781 aaagataata taccacctgt cacactatct aatccatatg atttagctag atccgaaccc
     3841 ataacaaaag ttgcataaat agccatcaat cccatcgtca tcctaaaggg taaaacaatc
     3901 gttgccgaat aaggggcaat aaattcattc catccaggaa acggtaaatt gataaagacc
     3961 ataaaaaatg tcccaatcaa aatcaatgac aatgttgaaa caaccccatt acgaattgct
     4021 aataaatgac gctgtgtacc aatttttgcc attggtggga caagtttctc ttcgacccat
     4081 ttcatcagct tttccactaa aaccactcct tttcaataaa atatgaacca ttcattgcat
     4141 gacaacgcaa agaatgttca taactaatta tataattaag aaattttatt tcaatatatt
     4201 ctcttaaata atcaaattta tttttaagaa tcctgaaaat aagttttcta aatcatagga
     4261 gcaacaaaaa aattcgataa ttaacaaaaa tcaaaatgat taaatctagt gattgaaaca
     4321 tatcatttca aaaaaatttt gtaaattcac ccataaatat caccctattt aacttttttc
     4381 atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt ttgttttaat
     4441 aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt ttgtgcttgc
     4501 tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc ctggttactt
     4561 tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc catatggatc
     4621 agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg aaatatatta
     4681 tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat agcgtatcca
     4741 actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg aataatttga
     4801 ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt ttatcacctc
     4861 tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag tatttttttt
     4921 gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc tttttgataa
     4981 gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc ctccatcaat
     5041 cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc agttgcacca
     5101 acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc cccttgatta
     5161 ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc tatatcgacg
     5221 atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat accgcggcgt
     5281 attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat attagcacgt
     5341 gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata ctctaagtaa
     5401 ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg aaaggttttt
     5461 tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt tattagtttt
     5521 tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga tgtataccag
     5581 ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata accgtaattc
     5641 actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat ttttacattc
     5701 ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata attcaaaatc
     5761 catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc tttgattgag
     5821 cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa tccatgttta
     5881 agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat ttgtgagaaa
     5941 acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat gcgaatattt
     6001 gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat atttctttct
     6061 tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt gttcaaaatg
     6121 gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat aatctgtttt
     6181 ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg ccataacata
     6241 agtcacttct aagccgatga taatttgata atttttttca gttggtttaa tgatcccgct
     6301 tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta cctctacccg
     6361 taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa acgcttgtag
     6421 catttttgtt gccatatatt cataattttt acttgctaac cctaactctg tttcttctaa
     6481 ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa tgtttctctt
     6541 attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc cgattggaag
     6601 aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa tacatgctcc
     6661 gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta aaccggcaaa
     6721 aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt caacaggctc
     6781 agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt tgaccatctc
     6841 tttattttct ttataagctg ctttatagat agctagacat atagctggta taccaaacat
     6901 cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt tgataaagcg
     6961 tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta aatcgtaata
     7021 aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat tcaaaaacat
     7081 aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat tatcaatccc
     7141 aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt gtagaatcat
     7201 caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg gtaatttttg
     7261 ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc caccaaaaat
     7321 ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag caatcgctaa
     7381 aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga ttgcaactcc
     7441 tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac caattactgg
     7501 gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg gcatcgctgc
     7561 aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat tcatttttat
     7621 tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca tttttcactt
     7681 tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga tacacaaaac
     7741 ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact tcgatcgttt
     7801 tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc gtattgattt
     7861 gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa taatcgacat
     7921 gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat ttttttgcta
     7981 atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga ctgtctaaat
     8041 gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat tcctgataca
     8101 gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg aactctccgc
     8161 tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat gttaaatgga
     8221 ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg aaacccggca
     8281 tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc ataatcccat
     8341 aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt cccatttcat
     8401 tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca tttactatgt
     8461 gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct tgctagatga
     8521 attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg ttcgatgata
     8581 tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc agtaaaaaaa
     8641 ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag gaatttgata
     8701 tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga ataggatttt
     8761 tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt taaactatta
     8821 ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc atttgaccct
     8881 aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat atcatattca
     8941 tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct atcttctctt
     9001 tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt tgaatctaat
     9061 ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc cataaattcg
     9121 attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga atctaataca
     9181 aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga aaagccaatc
     9241 cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc attatgattc
     9301 agctttttca tcacatacac cgttaattca cctcctctat atgataaaac aaaacaactt
     9361 gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa aaacgaccta
     9421 agaaagaacc ctttcttagg tcgtgcctaa ttg
//
LOCUS       DAYYAG010000107         9404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000107 DAYYAG010000000
VERSION     DAYYAG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.109"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACHARQ_002244"
     tRNA            2..74
                     /locus_tag="ACHARQ_002244"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACHARQ_002245"
     tRNA            78..150
                     /locus_tag="ACHARQ_002245"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACHARQ_002246"
     tRNA            155..225
                     /locus_tag="ACHARQ_002246"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACHARQ_002247"
     tRNA            238..311
                     /locus_tag="ACHARQ_002247"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACHARQ_002248"
     tRNA            323..394
                     /locus_tag="ACHARQ_002248"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACHARQ_002249"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACHARQ_002249"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8410643.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACHARQ_002250"
     CDS             1983..3455
                     /locus_tag="ACHARQ_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8410644.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACHARQ_002251"
     CDS             3538..4326
                     /locus_tag="ACHARQ_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8410645.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACHARQ_002252"
     CDS             complement(4393..5061)
                     /locus_tag="ACHARQ_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8410646.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACHARQ_002253"
     CDS             5176..6888
                     /locus_tag="ACHARQ_002253"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410647.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACHARQ_002254"
     CDS             6891..8663
                     /locus_tag="ACHARQ_002254"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410648.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9404)
                     /locus_tag="ACHARQ_002255"
     CDS             complement(8745..>9404)
                     /locus_tag="ACHARQ_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8410649.1"
                     /translation="PVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQS
                     RYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIE
                     LSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKV
                     SSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIE
                     I"
BASE COUNT         2823 a         1577 c         2039 g         2965 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cggg
//
LOCUS       DAYYAG010000108         9352 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000108 DAYYAG010000000
VERSION     DAYYAG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.110"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHARQ_002256"
     CDS             <1..1101
                     /locus_tag="ACHARQ_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8410650.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHARQ_002257"
     CDS             complement(1280..1615)
                     /locus_tag="ACHARQ_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8410651.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHARQ_002258"
     CDS             complement(1599..1862)
                     /locus_tag="ACHARQ_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8410652.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHARQ_002259"
     CDS             complement(2322..3125)
                     /locus_tag="ACHARQ_002259"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8410653.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHARQ_002260"
     CDS             complement(3103..4227)
                     /locus_tag="ACHARQ_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410654.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHARQ_002261"
     CDS             complement(4243..5058)
                     /locus_tag="ACHARQ_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8410655.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHARQ_002262"
     CDS             complement(5048..5920)
                     /locus_tag="ACHARQ_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8410656.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHARQ_002263"
     CDS             complement(6005..7294)
                     /locus_tag="ACHARQ_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8410657.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHARQ_002264"
     CDS             complement(7438..7950)
                     /locus_tag="ACHARQ_002264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410658.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHARQ_002265"
     CDS             complement(8017..8160)
                     /locus_tag="ACHARQ_002265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8410659.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8643..>9352)
                     /locus_tag="ACHARQ_002266"
     CDS             complement(8643..>9352)
                     /locus_tag="ACHARQ_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFM8410660.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQC
                     TISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWT
                     KYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLE
                     QLLSTVWLSVRTAFFVG"
BASE COUNT         2880 a         1996 c         1492 g         2984 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatgac tcgttgatga tttttactta tggtacattg gtttagcaga
     9061 ggacagttca cacattcttt tggattggat ttgtattctc ggtacccttc tcgagtaacc
     9121 gttgaaaaag cgagcatttg atcttgcgga caaatataac aatcaaaata ctcgtcatag
     9181 agaaactcat tttttgataa tagccccttt ttccctttgg gccttgaata aggcaaacaa
     9241 ggtcgaaggt tcaaatgagt caaaaagtga acaaacttag gtgtcttata cccagcatca
     9301 gcgacaacac aattgatttc aggaaaaagt ctcttcgatt gtttgacgag ct
//
LOCUS       DAYYAG010000109         9345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000109 DAYYAG010000000
VERSION     DAYYAG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.111"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACHARQ_002267"
     CDS             112..306
                     /locus_tag="ACHARQ_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410661.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ACHARQ_002268"
     CDS             575..874
                     /locus_tag="ACHARQ_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410662.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ACHARQ_002269"
     CDS             complement(971..1405)
                     /locus_tag="ACHARQ_002269"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8410663.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ACHARQ_002270"
     CDS             complement(1637..2347)
                     /locus_tag="ACHARQ_002270"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8410664.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ACHARQ_002271"
     CDS             complement(2337..3197)
                     /locus_tag="ACHARQ_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8410665.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ACHARQ_002272"
     CDS             complement(3202..3840)
                     /locus_tag="ACHARQ_002272"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8410666.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ACHARQ_002273"
     CDS             complement(3855..4154)
                     /locus_tag="ACHARQ_002273"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8410667.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ACHARQ_002274"
     CDS             complement(4197..5681)
                     /locus_tag="ACHARQ_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8410668.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ACHARQ_002275"
     CDS             complement(5701..6162)
                     /locus_tag="ACHARQ_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8410669.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHARQ_002276"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHARQ_002276"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8410670.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ACHARQ_002277"
     CDS             7465..8235
                     /locus_tag="ACHARQ_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8410671.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ACHARQ_002278"
     CDS             complement(8508..9335)
                     /locus_tag="ACHARQ_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410672.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2896 a         1919 c         1475 g         3055 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttcc
//
LOCUS       DAYYAG010000110         9301 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000110 DAYYAG010000000
VERSION     DAYYAG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.112"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1593
                     /locus_tag="ACHARQ_002279"
     CDS             142..1593
                     /locus_tag="ACHARQ_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8410673.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            1586..2308
                     /locus_tag="ACHARQ_002280"
     CDS             1586..2308
                     /locus_tag="ACHARQ_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8410674.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            2328..3479
                     /locus_tag="ACHARQ_002281"
     CDS             2328..3479
                     /locus_tag="ACHARQ_002281"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8410675.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(3827..4102)
                     /locus_tag="ACHARQ_002282"
     CDS             complement(3827..4102)
                     /locus_tag="ACHARQ_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8410676.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            4210..4974
                     /locus_tag="ACHARQ_002283"
     CDS             4210..4974
                     /locus_tag="ACHARQ_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8410677.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5098..5601
                     /locus_tag="ACHARQ_002284"
     CDS             5098..5601
                     /locus_tag="ACHARQ_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8410678.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            5866..6513
                     /locus_tag="ACHARQ_002285"
     CDS             5866..6513
                     /locus_tag="ACHARQ_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8410679.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      6556..6623
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6674..7942
                     /locus_tag="ACHARQ_002286"
     CDS             6674..7942
                     /locus_tag="ACHARQ_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8410680.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(8119..9300)
                     /locus_tag="ACHARQ_002287"
     CDS             complement(8119..9300)
                     /locus_tag="ACHARQ_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8410681.1"
                     /translation="MFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMMVTYFVVL
                     EVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLLIETQNIF
                     IGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLGVALTIFL
                     ILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQDYIVVLG
                     AGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAEAIAMKQY
                     ALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIFRAGLYAR
                     QAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFSALILKFA
                     Q"
BASE COUNT         2965 a         1614 c         1996 g         2726 t
ORIGIN      
        1 ataaaaaact tcttccatta tttttttaaa ttaatctttt tttattataa tagttatgct
       61 agtatattag caagttattt atgaatattt attcttttat agcttttctg attacattat
      121 tattcaaagg tggccaaatg gatgaaaaaa ggaataaaca tattattagt ggcgctgatg
      181 tttctactgg tttttttacc agtaaaagta caaggtgcag aaaaagatga aatttacgag
      241 cggataaaac aaagtggaga attagtcgtt ggcctatctg cagactatgc gccctatgaa
      301 ttccatgcga atgtggatgg taaggataaa atcgttggtt ttgatgtatc cattgccgaa
      361 aagatcgcaa aggatatggg tgtgaaacta aaagtagaag aacttggttt tgatgcatta
      421 ttaggtgcgt tgaaaactgg caagatcgat ttagttatat ctggtatggc ggtgactgaa
      481 gaacgtttac aagaagttga cttttctgac ccatatttcg tggtacagca aaaagtactc
      541 gttcgtaaag aggacaaaaa taaatttaag acgacagccg acttttctgg cttagccgtt
      601 ggggcgcaaa agcaaaccac acaagaggag ttagtgaaaa atgaactgac tggtgcagag
      661 ttgacctccc tgcaaaaagt tcctgatctg atcaataatt tgctgaatga aaaagttgaa
      721 ggtgttgtgc tggaacaacc agttgcagaa gcatatgccc aacagaataa gaaagtagct
      781 ttgactccag taaaatttgc gcaaggcgaa aaaaccacag ctattgcgat gtcaaaaaat
      841 acaccagaac tgaaaaatca tgtcaatgcg tcaatcaaaa cgatcaatga cgacggcttg
      901 ctgaaagatt atcaaaaaca agcgaatcaa ttgatgttcc aagatgatca gtcctttctt
      961 caaaaatacg gtccgtatta tttgattgga acaaaataca ctattttatt agcttttgtg
     1021 ggtgtattgt ttggaatggt ctttggtagt ttgcttgcac tgatgaaatt gggtaagaat
     1081 aaatttttaa aggcattagc cattatttat atcgaatacg tacggggaac acctctctta
     1141 gttcaaatct ttatcgttta cttcggaaca ggagtactgg gattggatct atcgaaactg
     1201 gtagcaggtt gtattgctgt ctcgttgaat agtgctgctt atgtagccga aattgtccgt
     1261 gccgggatca atgcggtcaa caaaggacag atggaagctg cacgttcttt aggaatggac
     1321 cagaaagaag ccatgcgttt catcatcttc ccacaagcaa tcaaaaatat tcttccagct
     1381 ctaggaaatg agtttgtcac gatcatcaaa gagtcctcag ttgtttcggt cattggggta
     1441 tcggaattga tctttcaaac gggcgtggta caaggtgcta gcttcaaacc attcctacca
     1501 tatgtgttgg tctctttgat ttatttcgtg ttgaccttca gtatttcccg tatattagct
     1561 ttagttgaaa ggagaatggg tctgaatgct taatgtaaaa aaactgtcaa aaagttttgg
     1621 cgagaatgaa gtcttaaaat caattgatct ttctgtgaaa gaaggagaag tagtggtgat
     1681 cattggccct tcaggaagtg ggaaaagtac aattctacgt tgtatgaatc tgctggaaga
     1741 accaacttct ggtgaaatct tttttaaggg acaaaacatt acagcccctg acagcgatat
     1801 caatcaaatt cgtcaaaaaa tgggaatggt atttcaaagt tttaatttgt ttccccatat
     1861 gaatgtactc gaaaatttga ccatcacacc tgtaaaaatc aaaaaagaag accctaaaaa
     1921 agcaaaagaa catgctctat cattattgaa ccaagttggt ctaaaggaaa aagtggacag
     1981 ttatcctacc agtttatctg ggggacagca acaacgggta gcaattgctc gagcattagc
     2041 aatgaatcct gatgtgatgc ttttcgatga acctacatct gcacttgatc ctgaaatggt
     2101 cggtgaagtg ttggcagtca tgcaggattt agctaaaaaa gggatgacga tggtcgtggt
     2161 cactcatgaa atgggttttg ctaaagaagt tgccgatcgt gtgattttta tggctgacgg
     2221 catcattcaa gaagagggaa caccgaaaga gatttttgaa aatccgcaaa atagtcgaac
     2281 acaggatttc ttgaacaaag tgttgtaaca aagatggagg aagtaagatg gacaaaagta
     2341 agattgcacg ccggtttcag caaccagcag aaaatctgtt gatgaatatt gctattttgg
     2401 caaaggaaaa agaggatatt atcgatttat cgattggtga tcctgacttg atcactgatc
     2461 cgacgattat tgaagcagct tatacagatg taaaaaatgg tgctactaat tatactgcgc
     2521 ctgatggcag caaagaattc ttgtcaacgg tcgtagattt ttatcaaaag aaatatggat
     2581 tgagcgttga atgcaaccaa gttcgagcta cagtcggagc aatgcacggg atgtatctag
     2641 ttttacaggc attactagat ccagaggatg aggtcatcat ccatgaacct tatttttcac
     2701 catatcggga acaagtcatt ttatctggag gcaagcctgt ttttgttcct acctatgaag
     2761 aaaagggctt ccagttggat gtggaagatt taaaagcagc gattacggat cgtacaaagg
     2821 tgatcgtcat caattcgccg aataatccta cgggagctgt tttttctgaa agcacgttga
     2881 aaaaaatcgc tgaagtcgca atcgaaaatg aactctatat catttccgat gagatttatg
     2941 aagactttgc ctttaaagag aagttcgtac cgatggcgcg atttgcgccc gataatacga
     3001 tcactgtcag tggtgtatcg aaggggtttg cgatgactgg ctggcgctta gggtacatga
     3061 tcgcaccagc atacatcaat caaattgctg ggttgatcaa tgaaaacatc acttattccg
     3121 caccgactgc ttctcaaaga ggtgcaatct acgccttaaa gcactatgaa cagcttgtac
     3181 cgccagttgt tgaagtattc aaagaacgat tagcttatat tgagaaaaga gtggcagaaa
     3241 tcccttactt atctttatct tctgtttctg gaagcatgta tgcttttatt aatatcagta
     3301 agagtgggct cgattcagta acgtttgtgg aagaggtttt agaaaaaagc ggcgtacttt
     3361 ttgtccctgg aaaagcgttt ggggaaacga tcggggacaa ctatattcga ctagcagcga
     3421 ctcagccatt ggaaatctta ggtgaagcct ttgatcgttt agcaaaacta aatttttaga
     3481 taaaactgta tatttgaaaa cagctatggg tatccattag aacaaagagg gaaaatgatc
     3541 ctttcgagag ttggcttgtt gactgggcag cgtcaggatt tgacaatgaa aaagtggtag
     3601 tggaaaatca aagtcgattt tccactacca taaaatatta tagactccat tttattgaac
     3661 caaaattttt gaagtcctcc cgcttttacg tatgcctatc actggttact aaactcgcta
     3721 ataagaatgt ttttttgtcg atctaggatg gagagagggg caaacttttt cgatctacat
     3781 acagacagtc cgatttttct gacatttcct attcatttct agaaggtcag cctattacaa
     3841 caaacttttt tctgtcctcc atcatcggat tttcctgaag gtctacatat tggacacgtc
     3901 cgctggggct tggtgatcgt ttgacttctt ccacgaattt tttgattgcc tcatcgtcgc
     3961 ccgcagcttc gatcgtgaca gagccgtcat cttcattttt cactgttccg gtgatcccta
     4021 attgatccgc tacgattttt gtcatatagc gaaaaccaac gctttgtaca cgtccttgca
     4081 cgttcatctt gattttgttc attttattca ccgttctttc ttttattttt aaattaatga
     4141 taccgacttt tttgctactg aacaagtgca atgatctaat ccatggtata atcagttgag
     4201 gtgaaaaatg tgaaagagat ccaatcagca aaaaatccaa gaatcaaaga attaaaaaaa
     4261 ttacataaga aaaaatatcg tgaagaaaaa ggggaatatc tgcttgaagg gtttcacctg
     4321 atcgaggagg ctgttgcctc aaatgcgaag gtcaacgaac tttttattac gcaacgcggt
     4381 ctgactgagt gggctagttg gcttgaagaa aatcaggcgc tttctgttac actagtatca
     4441 gaagaagtga tggcaactgt ttcagatcta ccaactccgc aagggatcat ggcagtggtg
     4501 gaagctgaaa aaagagagat accgaatttt gaaggtggtt ggttgttgct cgacaacgta
     4561 caagatcccg gcaatgtagg gacaatgatc cgcacagccg atgcttttgg tctatcttgt
     4621 gttgtactag gtaagggaac ggcggatgtc tacagtacca aagtattaag aagcatgcag
     4681 gggagtaact atcatttacc gatcatccaa gaggatctaa caaaactatt gcctatcctt
     4741 aaagagcaag gatttataac ctatggaaca gaactgaatg aagaagcagt gtccctgaat
     4801 gatatccaga caacagaaaa ttatgcgatc gtgatgggga atgaaggtca aggcgtacgt
     4861 ccagaagtgc tggcgctaac ggataaaaat gtgtatatac cgattcaagg aaatgcggag
     4921 tctttaaatg taggagttgc agcgggcatc atgctttatc acttctccgt atagttaagg
     4981 aactattaag ctttctgcat tttgcgaaaa cctcactaga atattacgaa cactagtggt
     5041 atactaagtt caatttcaaa gataaatgga attcaaaaag gtggcagatc gataattatg
     5101 acagaaaaat ggcgtgaaga cgaagaatat atgtcttata taaatgatct attagaaact
     5161 gatgcagtaa agaaattagc aaattacacg caacatgtcc actcgacacg attagagcat
     5221 tcaatcagtg tgtcttacta tagctacaaa ttagcgaaaa aatggggcgg tgatgcaaga
     5281 gcgactgctc gagcaggcct tttacatgat ttattttact acgattggcg tacgacgaag
     5341 ttcgatgaag gtacacatgc gtatgttcac ccaaggatcg ctgtgaaaaa tgctgaaaaa
     5401 atcacggagc tttcagatct agagcgtgat attattttga aacatatgtg gggagcaacg
     5461 attgcccctc ctaaatataa agaaggttat atcgttactt ttgtggataa atattgtgca
     5521 gtcaaagaag cagctcaacc aatgacagct tcaatgaaac aaaaatggca acgatatttc
     5581 ggtaaagaac aatcgattta aggtagttaa ctaagtattt gacccgagta ttttgaaaaa
     5641 aatcctcggg ttttttgttt agacattgac taattggtct ttttagtgtt tactttgttg
     5701 agatgaaaaa gtacataacc aacataaaaa taaagggggt gatgataaga tgcgatgtgt
     5761 atcttattta aaagagtttg agggctttat ctaaaggcac tttgatagaa agatcacttt
     5821 tttcttcaaa gtagccgatt ttgaaagaag aaaggactca aagtcatgga aaaaacaatc
     5881 aaaccatatc aatattattt tatcaagcga gaagtcgagc agctgttaag cgcctatagt
     5941 tcagtgaatg atccaaagac agtccaaaca gtccaagcat tagctgcgga aaagatccac
     6001 gaggtacttg gtcatcctaa tacagctgta aatgaattta tggaaaaggt actagacgtc
     6061 actctaaaaa aagaaaaagc agaacgacta ttagaggaat tgaaagtgat cgttcttcca
     6121 ttcatacaac cgacaaaacc gcagatcgat aaattattcc gaaaagtgaa aaagttaaaa
     6181 caaccagatt ggaatcaact ggatttaagg gaacatactt atattggttg gaatgatcca
     6241 ggtagccaaa aaaaatttat gatctattat gaagaagaaa aactaaaagg gatcagtgga
     6301 acattatctc ctacaatcaa caaaggaatt tgttcgattt gtcaaagaac ttcaaatgtt
     6361 tcactctttc tttctaccac aaaaactggt tcagatggaa catacacgaa aaaaggaaat
     6421 tatatttgtc atgatagtga ccaatgtaat cagcagttgg tacaattagc tcctttttac
     6481 gaattcacag aacgagtgaa acgtgagcgg tgatttcatt gtatttttct gaaaaatatg
     6541 ctatcttatt tggtgaagtg aataggtgat ggtgttcgcc tttaaacgtt gatcgtaaga
     6601 taactaatga cgcctactaa agaaacgggg ctaccccttt ttttagtagg cgtttttttg
     6661 aggaggaaat gagatggaaa atcgattacc tgatccattg aaggtgatca tctatatgat
     6721 caaatggtta ttgatcacag caattttagg tatttttatg ggaagtttat ccgcattctt
     6781 tctaactagt ttgactgatg tgacagctat tcggctggag catccttggt tactttttct
     6841 tttgccgata agtgggggac tttttgctta tctatatacg acgtatgggg gcaattcttc
     6901 ccggggaaac aatttagtga tcgatcaagg gaatggagga aatgaaaaga ttccattacg
     6961 gctgatccca ctgaccttat ttggcaccat taccacgcat ctttttggtg gttctgttgg
     7021 tcgggagggt acagcggtcc aaatgggcgg ggcacttgct gagaattttg ctcgttggct
     7081 aaaattgaac aaaatcgagc gtgagattct tgtcatcagt gggatcagcg caggttttag
     7141 ctcagtattt gggacaccct tggctgggac attgtttgga ttagaagtat tggcaatcgg
     7201 aaaagtgaga accgaagcac tgtttccaag cttttttgca gcactttttg cgaactttgt
     7261 aacggaaagt ttcaatgtca agcataccca ttatcaaatg ggggcgattc ctgcatggga
     7321 cccttcactt ttcttcaaat tgttgcttgc gggcattgca tttggtatca ttggctggct
     7381 ctttagccgt tcgatcgtct ggttaaaagc ttggtatgca aagcggatcc cccatccagt
     7441 tttacgcaac tttattggag gagcagtcgt ggtactcgta gtagtgattt tacagacgca
     7501 aagatatctc ggattaagct tacctttatt acaagatgca tttaatggaa gtgctcatcc
     7561 ttatgatttt cttgggaaac ttggctttac cgttctatca cttggcgcgg gataccaagg
     7621 aggcgaagtg acccctttgt ttgagattgg ggcaacttta ggtgcgacat tagcacccat
     7681 tcttcatgta tcagtacctt ttttagcagg attagggttt attggggtgt tctctggagc
     7741 aacgaataca ccgattgctt gctttgttat ggggatcgaa ctttttggag gtcaagcagc
     7801 cagctacttc tttatgatct gtttgattag ctttatgtgt tcaggaaata gtggaatcta
     7861 ttctgcgcaa aaagttgaag tgaaaaaagg aatcttgttc ctaccgatcc ttggaagaat
     7921 ctcacgacaa aaagagaaat aaaaagaggt tgggacagaa gtgtttaact ccgagaaata
     7981 agaagaaatt cacgaaaatt gctttttaaa tttttgtgaa tttcgcttat ttccgaagga
     8041 gttgcttctg ctcccaccgt ttattcggag ttagagtctg tggcaaaact gatttttagt
     8101 tttgtcccac gctctttttt attgagcaaa tttgagaata agagcagaaa atactgcaaa
     8161 gccaatcatt aagccacaga tgataagatg gcgttttttg tgtaagacaa ggatggcaat
     8221 aaattctctt aagatagcat tcggtaagta gtatagtgca gtttttgcac cgattccgtt
     8281 tgcattgagt ttcgcttgtc gcgcatatag tccagctcta aagatatggt aattatttga
     8341 agtaaaaatc gcgcgataag gcccattcat ttgctgatcc attatttctt ttgaaaacag
     8401 catattttct agcgtcgttg tggaattcgt ttctactaaa atatcacgct cgggaatccc
     8461 tttttctaaa gcatattgtt tcattgcaat ggcttcagct actttttcat cggttccttt
     8521 tcctcctgac atgagcaaga ctggcggatt caaagttgct ttactttgtg cccaataaaa
     8581 tgcgatcgcc ttattgatcc ttttagcaag taggggagat acgcgttcac catcaatcaa
     8641 gccagcgccg agaacaacga tataatcttg gttatacctt ggacggttga actgatacaa
     8701 aattgaaaca gtcataaaat taaagaaaac gatgagtaga taagcaaaaa taaacggaat
     8761 gacagcaaat agagcatttg cccattgtgg gagccaatgg actaatgtat agtccaatat
     8821 caaaaagata gtcaaagcga ctcctaacaa gagagtcaaa aggttggcca aagaacggga
     8881 ttcttttttc atgactacaa caccattcca aaataaaaaa atgatcaaaa tgtagatacc
     8941 gattgccaaa agaattaata ttggtatccc gatcaaagcc aataacccac cgataaagat
     9001 attttgcgtc tctattagta gccaccctag ataaccacca aagacaatca aaaacgtatt
     9061 aaaaagtacg ccattgatta acctacgttt ttccttaaaa tagaaaaatg caaaaatact
     9121 gaaaaatagc aatggaatca aaaaataaaa tgtatgataa aaaacttcta aaacgacgaa
     9181 gtaagtgacc atcatcataa aagtccacgt cccacaataa aagaatagag cgggatcgct
     9241 ttggcgcttg cgccaataga ggatgcctga cattcctaaa atatagataa ctaaaaatat
     9301 c
//
LOCUS       DAYYAG010000111         9239 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000111 DAYYAG010000000
VERSION     DAYYAG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.113"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..629)
                     /locus_tag="ACHARQ_002288"
     CDS             complement(<1..629)
                     /locus_tag="ACHARQ_002288"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8410682.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEA"
     gene            complement(639..2015)
                     /locus_tag="ACHARQ_002289"
     CDS             complement(639..2015)
                     /locus_tag="ACHARQ_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8410683.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(2135..2782)
                     /locus_tag="ACHARQ_002290"
     CDS             complement(2135..2782)
                     /locus_tag="ACHARQ_002290"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8410684.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2866..4293)
                     /gene="uxaC"
                     /locus_tag="ACHARQ_002291"
     CDS             complement(2866..4293)
                     /gene="uxaC"
                     /locus_tag="ACHARQ_002291"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8410685.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(4367..5446)
                     /gene="uxuA"
                     /locus_tag="ACHARQ_002292"
     CDS             complement(4367..5446)
                     /gene="uxuA"
                     /locus_tag="ACHARQ_002292"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8410686.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(5449..7080)
                     /locus_tag="ACHARQ_002293"
     CDS             complement(5449..7080)
                     /locus_tag="ACHARQ_002293"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8410687.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7384..8055
                     /locus_tag="ACHARQ_002294"
     CDS             7384..8055
                     /locus_tag="ACHARQ_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8410688.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(8215..8406)
                     /locus_tag="ACHARQ_002295"
                     /pseudo
     CDS             complement(8215..8406)
                     /locus_tag="ACHARQ_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(8774..9115)
                     /locus_tag="ACHARQ_002296"
     CDS             complement(8774..9115)
                     /locus_tag="ACHARQ_002296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410689.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT         3045 a         1799 c         1201 g         3194 t
ORIGIN      
        1 gcttctagat caccaggaga atcaccaatc attaatatct tatccaaatc acaaccattt
       61 tcaattacac ttcgaataat atcttctttt tttcctttat cttgacaaca cagttcatca
      121 actaatgata ataattcatg tctttcccat tcttcttgaa cagcttcctt attagctgag
      181 cttacaataa ttatcttccc atattttttc agagctttta tagtatctct agcacttaaa
      241 aagggtttat ctaatccttt tgcttgacta atctgttgat tgacttcttc cgaccatttt
      301 aaagctttta ataaatcctc tgactgagaa tattctattt ccctcttcaa agatggatta
      361 gacaattctt ttgttagaga tacccagtgc tttaatcgat taatcgatgt tacatcattt
      421 ttttctaatg ttttaactaa acctaaaaat ctgtttattc cacgagtctc tgaatataaa
      481 ttaatattat tccaatcctg taggaatttt tctctatgat gaacatcaaa aatctctgca
      541 gcaattggtc cgaaaaagag attgtgttta taagtcatcg tatccataac acagccatct
      601 gaatcaatac aaaatatcca attattcata tttctctcct atttatgttt ttgttttaaa
      661 agctcgcaat tttctaagac tttatcttta gataatggat aaaggaaaaa taaaattaat
      721 cctacaatca gaaaacaaat tgatggaatt aatgttgaag cgttatataa cttttctaaa
      781 acatctcccg tttgtacatt tgctaaagag tcatagccta taatagaaag agtccaacct
      841 ccgacgcctc cagctagagc ttgacctatt tttctagcaa aagaatatac cgcatatacg
      901 gtcccatctt ctctttgatc agactttact tcttgatcat caataacatc agtaatattt
      961 gcccaaataa cagtattaaa cacacccaaa cccatatagc caaccgaact aagaattaag
     1021 aatacataca tattactagc ttttatcaaa aataagatca aatatactat tgctgaaaag
     1081 aacattccta ctgatgccag ttcttttttt ccatattttt tacttaaaaa caaagttaaa
     1141 ggcgaaacaa ctattaaaac caaaatagga ttgataaaat tcattaaaac tattccctgt
     1201 gcatcattat agtaatcagg aaatacataa ttattcatac tcataattaa taattgagca
     1261 aggagtaaga aaatagccgc accaattata gaaagtaatg ccctattttt aactaaccct
     1321 tttaaagatt ttaacaatga aactttgtat tgagattggt caattcttac tcgttcagta
     1381 gttagtttat aacatattaa ataacaaata agtgcacata tcgaaaatat tcccgcaaca
     1441 attgtaaaag ttgatccacc acgaactatt tgagcgcctg aatcatctct tgtataaata
     1501 aataatggtg ttaatacacc aatgaccaaa cttgctaaag tagctccaac atttcggaat
     1561 gtcgatagca ttgttctttc ttctgtaata gaagtaatag ctgatgccat tgatccataa
     1621 ggaatattta tagatgtgta aaaaatactc ccccataata tatacgtaac atacatgtat
     1681 actaccttaa cgctcataga agctgaaatc atagaagttt gatacattaa aaaacttgct
     1741 aaagcgactg gaccactcat tcttaaaatc cacggtttaa acttaccctg cttagttgct
     1801 ttcgatctat caacaatacg ccccatagtt acatcagtaa ctgcatcgat aaatcttgct
     1861 actaaaaata atgttccaac ggcagctccg cttatcccca atacttttgt ataaaagatc
     1921 attaaaaaag aactagcaaa aatgaaagta aaatcgtttc caaaatctcc aaacatatat
     1981 cctattttgt ctcttatacc aaattttctt tccatacaat tctctccctt ctgttgttaa
     2041 gcgttttcat tctgatgaaa gtacgtacta tactctgaag cgtaaagagt cagtatttat
     2101 tttttacatt tcagagtatt atttgttttt tctcctaata attctttatc tcataaaact
     2161 tatctatata tttactagct actcgactta cttcttcaaa atcacctttt tcaacagaag
     2221 ccaataaatt cccacctaca ccaactgcaa caactcctac ttcaaaccaa tcttctaagt
     2281 tctctatact tactccgcca gtaggcatta tatttacgtg tggtaatgga gcttgatagg
     2341 cttttacaat ggatggacca aaagcagaac ctgggaatag tttaatgata tctacgccag
     2401 cagtaagtgc ctcctttatc tcagtaattg tcatacaacc tggtaaataa ggaatttgat
     2461 ataaattaca aatctcagct gtttgtcgat caaaagatgg actaacgata tattctgccc
     2521 cagccataat tgcgatacga gcagtaaacg catctaaaac cgtaccggct ccgattatta
     2581 catctttttg atcagcatat tttttagcaa tttctttgat tatttcttca gcattaggaa
     2641 ctgtaaaagt tagttcaatc cctttaattc cacccgcaat aattgaattt attgctttaa
     2701 tggcctcatc cttatttttc ccacgtacaa ccgcaatgac tccagccgct tctaacttac
     2761 ttaaaatttc tacttttttc atatctatct ccttccttta tggatacata ctttgtctag
     2821 ctgtactaac ttacaaagaa taaatttaag cttttatttc aagtattatt ttctacttcc
     2881 gatttcaaaa aaattaaaat aattatacgc attacgataa caaatattct ccactatctg
     2941 actaagatac atttcatcat ctggaattcg gcctgcttta acccactcgc ttagtaattg
     3001 acataatact cgacgaaaat actcatggcg aggatagctt agaaaacttc gagaatcagt
     3061 caacatacct gtaaaattcg acaacaaact ttgttgtgcc atcgtagtca actgttgacg
     3121 cattccttca gctgtatcat tgaaccacca agcacatccc aattggattt tttgaatcat
     3181 acccccttgg aagctttgca ttgctgttgc tagtggaagc caatcagttg gatttaatga
     3241 atataaaatt gtttttggta agcacccact cttttcagcg gtatcgaaca atgctacaac
     3301 attttgtgtc aaactagctt gatcccccat cgaatcacca ccgacatcta ctccaatttg
     3361 ttcctttaat atacttgaat tatttcttaa agcatttaaa tgcatttgca tcgtccaacc
     3421 atatcttgta tactcagaca tcaaatgtaa ttgaatacct gtaacaaatt gtgcaatttc
     3481 tgataaatgg aaatccgttt ttcctgctaa agcttttatt aacagattgt cgagtatttt
     3541 ttcatctgct atttcataaa aataagtatt caaaccgtga tctgctaatc gacagccaat
     3601 ttgatgaaaa tgatccactc gttggctcaa tgcttgcttc aacccattaa agtctttgat
     3661 tggtatagcc gctgtttgac taagttttaa aatatagttc gaaaagcctg atgatttgat
     3721 actccagacc ttatctggac ggaaagtagg taataccttg aaagaattat cctgtttaga
     3781 aagctcagta tgataccaca agtcatctgt aggatcatcc gttgtacaga tggctttcac
     3841 atccatcatc tccaacaatt ctttcgggcg ataggaatcc gtttgaattt tttcgttagc
     3901 acgtcgccaa atttctggtg cattcttctc agaaattgtc aaatcaatat caaagtaacg
     3961 ttgtaattcc aaatgagacc attcaaaaat aggattacct ggcgccttct ccactgtttt
     4021 tacaaaagct aaaaattttt cataatcatc acctggacca ctgatcaact cttcttctat
     4081 accattagct cgcatcaatc gccatttata atgatctcca gctcctttat catttaacca
     4141 tacctgagtc aaattcttgt attgcttatt ttctagcaaa tatgctgggt ctaaatgaca
     4201 atgatagtcg ataattggtt ggtgttttgc aaatttatga aaaagtcttt tagctatttc
     4261 attctctaat aaaaagttct tatctaaaaa catatttaaa cactcctttc cttccgttag
     4321 tttttagaag agaaaatttt tcctctaaat aaactccctt atataattat ttactcgccc
     4381 ccttttcaag cgcttcccaa ataccattta aataaactgc acctagagct cggtcatata
     4441 atccataacc aggtctaccg gtttctcccc aaatcatccg accatgatct ggacgaatat
     4501 agccattaaa tccattatcg tacattgttt ttaatatctt atacatatct agagaaccat
     4561 attctgattt atgcggcgct tcgtaaaaat cgagtccatt gtcacttaaa tatttgatat
     4621 tacgaacatg tgcaaaagga atccgatccc gttgacaaaa ttcagataga atttcataga
     4681 catcattatc tggtcgttct gcaatacttc cagtacataa tgtaatacca ttagaaggag
     4741 aatccactac attacacaac caatctaaat cgtcacgatt tttaacaata cgaggtaatc
     4801 cgaaaatgga atatggtgga tcatctgggt gaactgccat tttaatccca acttcttcac
     4861 atgtcgggat aattgctttg agaaaatatg caaagttctc tcgtaatttc tcttcatcaa
     4921 cttctttata ggcttctaat aattgcttaa tttgtgataa ccgatgaggt tcccaacccg
     4981 gcaattcaaa attattagta gaattttcta cttctctaat aatttcctcc ggatcagtag
     5041 ggatattttt tgaaatatag gctaacgtag aagacccatc atctaaacga taatccaaat
     5101 ccgattttac ccaatcaaat actggcataa aattataaca gataacctca atcccaaatt
     5161 ctgccaaatt gcgaatcgtc tgtttataat tctcaatgta ttgatctcgt gttggtaaac
     5221 caattttgat atcatcatga acattaacac tctcaataac tttcaaggta agtccattgg
     5281 cttcgacttc atcttttaaa gatttaattc gttctctcgg ccaaacctct ccggctggta
     5341 tatccattaa tgttccaaca attcctttca ttgctgggat ctgacgaatt tcttgtagtg
     5401 agattttttc ttcatttgcc ccataccatc taaaagtcat ttccatagtt actttcctcc
     5461 aacttctgtt atatatttat tgattgtttt acgaactgct tgaggaccta ctagcatttc
     5521 gcaaaagatt ttttcagttt tctttcctac cccaacttga tataaatcta aaccaaagat
     5581 ttctttattt tctaaaattt cctgtaccgc acaatgaatc tttgaaggat cttgttcacc
     5641 taatttcaat actgataatt tatcctgcaa ctctaccaat aacggatcag gacttggcac
     5701 aaaattttcc ccttgatcat cgactgccag taaataacgt aaccaagcag caatagcgat
     5761 aggaataaat gttagatttt ctgcatttcc gtctttttcg acatactttt taattgtttc
     5821 accatagcga atcgctactt tttgagaagt atctgttgca attcgttgtg gtgtatctgg
     5881 aatataggga ttaggcaaac gtttattaat tacttcatca ataaattgtt ttggatcaat
     5941 aatgcctgga ttttcaacta ctggtaaacc ttctaaataa cctattcctt tgattaaacc
     6001 aaccaaatca tcatcctgca tctctgctga aattgattga tatcctagca gacaacctgt
     6061 tactgccaaa gcagtatgaa gtggattcaa gcttgtcgta accttcatga tgtctgcttt
     6121 atcaaccgtc tctctatctg tcataatcac accagccttt tccaatttag gccggccatt
     6181 tggaaaacta tcttcaataa ctaaataatg aactacctca gtattagtga aagcagcaat
     6241 attggtatgt ttttttgtgt gaatgatatc taaatcctcg agccccatca tcaatagatc
     6301 ttcctttaca ttctctgaag gatttggcgt aattcgatca atcatactcc aaggaaaagt
     6361 cactttttct ttatccttta aataagcaat aaatttttca gaaacatatc cttctttttg
     6421 ccaagcttct gcaatcgtaa aaatactatc ttgaaaacgt tgaccattct gtgagaaatt
     6481 atcagtagaa accatcgcaa tcggcaactc tccagcatta aatctgctat ataacaagga
     6541 tgttacaata ctcatcgtat gttgaggatt gctaggtcca gatttgatat cagctttgac
     6601 taccgaaaag aattgccctt tagaatcttt taaactgtaa cctttttcag taatagtaaa
     6661 cgttgtaaac tgtaatttag gatcttcaaa atatttaacc actctttgat agtccgctat
     6721 attcttcgga ttgcaatata aactatcagc cgttactgcc aaaagtcttt ttttgagttc
     6781 accatctcca ctcataacaa tttccaaaat gtcattgtta tagtcatgat aagctttttc
     6841 cactacttct tcatcaaatg tttcacacac aactactcct gtttctaatt cattttgatc
     6901 tgctaagcat tgagcaattt ctgcatgaaa tcctcgataa agatttcctc caccaaaatg
     6961 aagccatact ggatgttgct ggccttcttg cttaacttct ttgacatcaa aacacggcag
     7021 tagaatctgc atttcattga attcttcgat agaattttgc caatttttaa tcgaaaccat
     7081 aattaacctc ctcaaactga tatgttagtt ttgatacatt ttatatttta aaacccattg
     7141 taaccgttgt caatataaaa agggaataaa cttgcttttt tctaccatat cagaatgatt
     7201 gtatttttta ttcagaaaaa taaaatactc cttctttttg gactgatttt atcgaaactt
     7261 attttttctt tttttcatgt attcttttat ttttataact agtgtattac ttcaaaattt
     7321 gatattatat ggcatgaaca gaattaaaat atcagttaaa ataaaagtta aggagcattg
     7381 tgtatgacaa ttaaaactag tctccaaaat caagcttacc aatctattcg ccgacaaatt
     7441 atctatgctg acttagaacc tggaaaaaaa gtatcagaaa aaaaattaga agaaacactt
     7501 tctattggtc gtacaccaat acgtgaagca ctgattcaac tccgacaaca aggtcttgtt
     7561 gatacaattc ctcaaagcgg aaattatatc tcattaattg atttagattc ggccaaaaat
     7621 gctcgatttg tcagagaaca tttggaacga caaattatgt tagagtgttg tgccaaaatg
     7681 aataaccaaa aaaaacggat tttagaaact ataatttcag aacaagaaaa agctgttagt
     7741 caaaaaaatg ctcgtagttt ttttcataat gataatttat tccataaagc ttgttttgaa
     7801 attgcagagc gagaagaagt atggcaatgg ttagaaaata acaatacaca cttagaacga
     7861 tttcgatggc tacgagtaaa aacggaagga ttaaagtggg aaaaaattat ggaacaacat
     7921 tatcaactat ttgaggcatt aacaaaacaa gatccagaag aagctaactt tctaacatcg
     7981 ttgcatcttc atttaatgct taatgagcaa aatattgttt tagctactca tccaaaatat
     8041 ttcaaaaata tataactttt aactcttttt aaattaaatg gttgttctga tcagaaaaat
     8101 atcgcttgtt ttcttattat gtaatatatt tcataaacta ttctgcataa caactattgt
     8161 ttgtactttt tacattggat aactaattat gactgctgat aacttttgat attaaactag
     8221 tatctccaat acaaataaaa aatagatatc ttgttcatca aaattagttc gtggatactt
     8281 accttcattt acatcattca gcggtttccc attttctttt attctattat ttcattttct
     8341 aatagacttg gcttcatttc gcaatacttt ttttcaatag gcatcaaatt ggtgttgttt
     8401 agatatttag ttgtagtttt gtgacatgat aagtatgctc ctttttctct tttccactaa
     8461 tacaacgatc aaagggtgct tttttcaaaa aactagaaaa atcaaaataa gattcgtatt
     8521 ttgattctat atatcctgtt tcaactacat cttatatttc acaatagaat aaatagcctt
     8581 ttgcatagct gcttaactta taagaaattg caatttactt cttcaaagtg aaaacgaata
     8641 tgagtaaaat aaaattattc ttcttacaga taattaaaaa tagcgcgtaa caaaaatcgg
     8701 cattcaatca cataattagg attgatgccg attacttata ttctacgtat agttttagta
     8761 ttcttatttt ttatcaccag taaccaattg agaacagccc taaagcagaa tagtctttta
     8821 tctcaaaacg cttaatttca ggcttaggaa tatgaataat cttttgagta tattctttct
     8881 cttttaatct tactgaagaa ataccactgc ctatcttttt tacaaatatt tcatttggtg
     8941 tacagactaa taaaacaata tttgtaatca atccttgcac taaagaatta aaaggaagac
     9001 tatttaaaac agggtgtgca taactataac atgtgaagta gttattttga actttaactt
     9061 ttaaaaactt taaaaataat cttatttgtt cttctaaaca atcatctata ctcatataat
     9121 cactccacta attaatctcc ccaaaaagac acagcattaa gagatatacc aacacaacag
     9181 tgcaaataac tctacatctt ccttcgcaac cttatttcaa taaaccataa aaattattt
//
LOCUS       DAYYAG010000112         9147 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000112 DAYYAG010000000
VERSION     DAYYAG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.114"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..264
                     /locus_tag="ACHARQ_002297"
     tRNA            191..264
                     /locus_tag="ACHARQ_002297"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:225..227,aa:Ile,seq:cat)
     gene            complement(649..1146)
                     /locus_tag="ACHARQ_002298"
     CDS             complement(649..1146)
                     /locus_tag="ACHARQ_002298"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8410690.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1186..1995)
                     /locus_tag="ACHARQ_002299"
     CDS             complement(1186..1995)
                     /locus_tag="ACHARQ_002299"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8410691.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2329..3186
                     /locus_tag="ACHARQ_002300"
     CDS             2329..3186
                     /locus_tag="ACHARQ_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8410692.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3179..4081
                     /locus_tag="ACHARQ_002301"
     CDS             3179..4081
                     /locus_tag="ACHARQ_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8410693.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            4114..4464
                     /locus_tag="ACHARQ_002302"
     CDS             4114..4464
                     /locus_tag="ACHARQ_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8410694.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4513..5436
                     /locus_tag="ACHARQ_002303"
     CDS             4513..5436
                     /locus_tag="ACHARQ_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8410695.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5514..6407
                     /locus_tag="ACHARQ_002304"
     CDS             5514..6407
                     /locus_tag="ACHARQ_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8410696.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6445..7008
                     /gene="amaP"
                     /locus_tag="ACHARQ_002305"
     CDS             6445..7008
                     /gene="amaP"
                     /locus_tag="ACHARQ_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8410697.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            7022..7210
                     /locus_tag="ACHARQ_002306"
     CDS             7022..7210
                     /locus_tag="ACHARQ_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8410698.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7223..8125
                     /locus_tag="ACHARQ_002307"
     CDS             7223..8125
                     /locus_tag="ACHARQ_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8410699.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            8157..8399
                     /locus_tag="ACHARQ_002308"
     CDS             8157..8399
                     /locus_tag="ACHARQ_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8410700.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8655..9026)
                     /locus_tag="ACHARQ_002309"
     CDS             complement(8655..9026)
                     /locus_tag="ACHARQ_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410701.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3203 a         1566 c         1762 g         2616 t
ORIGIN      
        1 acattgagaa taaataaaag aataacattt tttctaaaat aagcaaataa aaattaaagt
       61 aacaaaatag ttatgacaca agtaaaagaa ataagaataa caatattatt ttacttatat
      121 tttgaaactt gtgagagttt gtcattcgtg ctatagttga aaagtaacaa aaataaattt
      181 tatttatgat gtacctttag ctcagttggt tagagcagac ggctcataac cgtccggtcg
      241 taggttcgag tcctacaagg tacataagag gttgtgaaaa agctgccctg catcaagtaa
      301 taagaacagc caagtaaaaa cagcttctta tgtttttact tggctgttct tattactgca
      361 gatgcaagct tttgaatacc gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag
      421 tattagctta acaaacggaa aaatagttcc tcatattttg ccgtttgtta agctaatacc
      481 gagggtcagc tgattgaaca ccatttaaga aagaggtcgt gagcctgccg tttcactctg
      541 tgtcataaaa aagccaatct actttgatag aaaaagcacg taggactaaa acagttctgc
      601 gtgctttttt tattgtgtgt taaatatcgt aaatgaagat agatattttt atgattcgac
      661 gatagagacg ttgaaatctt tcaacagctc taaagcggat tctccgtgtt ttctactgga
      721 gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag
      781 attagagagt acacacaagt ttgtttcaat cccgactact tcgatctctt tttgttcttc
      841 tttgctttca aataaagttt cctggatttc agctaaagtt tcaggtggaa tcgtaaagta
      901 atacttttta atcactcgtt cattttcttg tggagaaaga agagggatca attgcaaatc
      961 ttctcgttct tcttctttgt ccttcagttc aactggtata tcccgtgtga agagaatata
     1021 ttcattattt tggcgtgctt tagctaatct ttgagcaata cgttctgcca attgatctga
     1081 ctctggaata taaaattcgc tgtttggatc aagaatatga ttttgcatat caataataac
     1141 taacattttc tttcctccta cattcatatc gtcattttat tctttttaat agaagttttt
     1201 taaaatataa gcaacaccag cttcttcatt cgtcagtgtt acttggtttg caatttcttt
     1261 tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg ctgtaattca acatagcaat
     1321 gtcgttttct tgatcgccaa aagccatgat gtcactcgat tcgattccaa ggtaatccgc
     1381 cagaaaaaca agtgaatgag ctttagtgat acctgtaggc gtgatttcta agaaatgcgg
     1441 atcagagaat actgattgga taccatctgc ttgcagatct ctttgtagct tctgtagttt
     1501 ttcttcactc ttatctgtta atgctaattt aagatagcta agagaaggat cttccaacat
     1561 atttaaaatt tgatcaaaag aatggttctg taaattgaaa tgttcataat aagggtcatc
     1621 tttgactaga tttgattcat cgaatgtcag ctgatcatct tgggtttctc ggctagcaat
     1681 gatggatatt ttcgcttctt gagcatagaa gaaggctttt ctcagtgtat cgctatctat
     1741 ttttgaacgt ctgagaactt tcgccatttt tccatccttc atttcagcaa tcagtgaacc
     1801 attggaagaa atcaaatagc caccgattcc taattcggaa aaaatatctt tagcagaaat
     1861 gctgttcctt ccggtagcga cggttacgat tccgccattt tcatcaaatt tccgaagtgc
     1921 ttggatattt tcttctggta tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat
     1981 cgctgctagt ttcattttat cttcctactt tctttttaca tatctatttt cctgttacct
     2041 taagattaaa tgagaattta aaaaaatgaa actaacttgc tttgtcatta tgaatttttt
     2101 caaaaaacat tcttaagaaa aaggtggcta tatatagtag tttttggtca atacctttga
     2161 ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg ataaaaaaat
     2221 tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat gaggaatatt
     2281 ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat ggagaagaga
     2341 tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta cttgagcgtg
     2401 atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt ttatgtgatt
     2461 gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa taaatattca
     2521 agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga cttcctagtc
     2581 agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt attagctaaa
     2641 gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga cgatatcgta
     2701 aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga tccaggccaa
     2761 gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa ttcaggagtg
     2821 atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac tatccattac
     2881 ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa tgctgtactt
     2941 tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct gatttttgaa
     3001 cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa tgaaaaacct
     3061 tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga tcatccgtat
     3121 accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg cgagatcaat
     3181 gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac cacatttagc
     3241 tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc ggttctatgt
     3301 gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag tgaaaaacgc
     3361 acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta caaatgtggt
     3421 aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg aattgttccg
     3481 cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt tacaagatat
     3541 ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg aagacgctgg
     3601 gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt atttcaactc
     3661 aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc aaaaagtatt
     3721 agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg cattaaatgc
     3781 tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag gctatatcat
     3841 ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag aaactcgcaa
     3901 caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg attttgaagg
     3961 gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt gtgtagctaa
     4021 attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag ttgttaaata
     4081 atagaagacc acaactgaag agggggagta ggaatggaaa ttacaatgtc taaaaatgca
     4141 gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc gcttatcaat
     4201 gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt ttttcaaatc
     4261 attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc attattggac
     4321 atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat ggacttcaac
     4381 aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta taatatcaaa
     4441 ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag gtatacattg
     4501 agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa aaaatataat
     4561 tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga agaattcgag
     4621 cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg gatggagcgc
     4681 ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct agatcagtca
     4741 gataatcgta tcgaaaaaaa attaacgcca atatcttttg tactttccga taatctcttg
     4801 attacttgta tcgatagaaa aaccgaattt gtggatcggc tgctgacaaa gcatggaaat
     4861 catttaaact cgtttgaaaa gatcatcacg tatacattat tagatattta cacccattac
     4921 gtgaaagaac taagagagat gaaaaagcga attgacgctt tggataaagc agctcgtaaa
     4981 acaacggaaa atgaagaact gtataaacag gcagatcttg aaagagacat cgtatatatc
     5041 gatcatacct taagagatca acgggaaaca atggatcaat tatggaattc tgaaactttc
     5101 atcaaacgtt tagacgatga acagctactc tatgatgtcc atttgagaca gcgacaaaca
     5161 gaaaaaatga tcgagatcta tcgagattta ttagagagta ttggtgatct attcaacggg
     5221 atgatggaca ataatctaaa tcatatcatg aaatatctag attcagctgc attaatcatt
     5281 tccgttccgg cgttgttcgc agggatttgg ggaatgaata caggaggatt accgggtaaa
     5341 agttctgctc taggctttat actcgtagtc tttggctctt tgatagcagc agtagtttta
     5401 ggttaccatt tataccggaa agattataca aattaaagaa tctatttaaa acaggtatca
     5461 aacaaagaaa tttgttttgg caatacaatt taaaaactag gaggaataca gcaatgacag
     5521 aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct aaacaagatc
     5581 aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga gaagaatcct
     5641 atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga gattcaggga
     5701 tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct cttcaattct
     5761 tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagat gcaggacgta
     5821 agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa atcatagata
     5881 aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga cagcaaattt
     5941 cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt atgataaaca
     6001 tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca ggaggagcaa
     6061 tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta gattacgcag
     6121 caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt gcacctaaag
     6181 gaatccgtgt aaatggtgta gcacctggtc caatttggac accattacaa ctagacgaag
     6241 gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa cgtgcaggtc
     6301 agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca agttatgtta
     6361 cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa gttgatcgtt
     6421 tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg agtgatcgga
     6481 acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac ctatccattg
     6541 ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt gcaacaatat
     6601 gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact tgttttcctg
     6661 ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga gttgacgttg
     6721 gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga attcatccat
     6781 tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt aaaaggggag
     6841 ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga aatcgaaaat
     6901 gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa atacacaggc
     6961 tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg ggggaccaaa
     7021 aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac ttggattgat
     7081 attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct tgatcatggc
     7141 agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag atcactattt
     7201 taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca ataaaatgaa
     7261 caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat caaatggtgt
     7321 gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag tagtccctaa
     7381 cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac ctcatcatcc
     7441 aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt ctaaacctgt
     7501 ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta gttctgacca
     7561 cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa agcatgattc
     7621 tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg tgcaaaaaat
     7681 catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg gtttcttctc
     7741 taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg aaacagaagt
     7801 cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca aagatatcga
     7861 aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata tgacacattt
     7921 agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag aatttgaaaa
     7981 agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg gtgaatttgc
     8041 ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa aagtaaaaga
     8101 aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa cgaaacatgg
     8161 gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga gcaatcacga
     8221 ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt ggatcagcac
     8281 ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca atcgtgccat
     8341 cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga aaactgtaaa
     8401 aagactttta aaaagaggtc gtgaaaaagc tgttcttcat caagtaataa gaacagccaa
     8461 acaaaaatag cttctcatat ttttcatttg gttgggcata ttatcgaaga tgcaagcttt
     8521 tgaacactgt ttatacggaa taagaggctg tgacaagact tttgtcacag cctcttattt
     8581 atgcgggcaa attcccaatt agatttttaa aagtttttcc ttaaatgtgt ctttattgat
     8641 acagagaagc ttctttaata aaacaatgcc tccctaaatt ttcttgagat aggaaaggtg
     8701 aacatacaaa ataatacgac cactttgaaa acccagatga tccacaaatg gatcgtatga
     8761 tactgatgaa gtaaaaagca atggatcaga aactcaaaga gattgaaaaa gacaaggaag
     8821 aaataatatc tcttttttac tcttgtctgt tttagccaat aatagctcgt aaagtaatct
     8881 agacaaacaa atagaagtaa acaaagtttc gtaagagcag caatcgtatc atgttgctct
     8941 gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa tatagatcac gagtaatgaa
     9001 agaaaaaagt aaacaataaa tggcatacta ttcctcctaa ttgtgtgagt tcgccctcat
     9061 tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc gtaacaaatg ttatctaatg
     9121 tatttttaaa taaataatca ttattat
//
LOCUS       DAYYAG010000113         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000113 DAYYAG010000000
VERSION     DAYYAG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.115"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHARQ_002310"
     CDS             complement(13..1152)
                     /locus_tag="ACHARQ_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8410702.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHARQ_002311"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHARQ_002311"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8410703.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHARQ_002312"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHARQ_002312"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8410704.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHARQ_002313"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHARQ_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8410705.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHARQ_002314"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHARQ_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8410706.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHARQ_002315"
     CDS             complement(6166..7257)
                     /locus_tag="ACHARQ_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8410707.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHARQ_002316"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHARQ_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8410708.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHARQ_002317"
     tRNA            8800..8871
                     /locus_tag="ACHARQ_002317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAYYAG010000114         8932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000114 DAYYAG010000000
VERSION     DAYYAG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.116"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..870
                     /locus_tag="ACHARQ_002318"
     CDS             274..870
                     /locus_tag="ACHARQ_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM8410709.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            851..1297
                     /locus_tag="ACHARQ_002319"
     CDS             851..1297
                     /locus_tag="ACHARQ_002319"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8410710.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1640..1963
                     /gene="relB"
                     /locus_tag="ACHARQ_002320"
     CDS             1640..1963
                     /gene="relB"
                     /locus_tag="ACHARQ_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8410711.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2283..2510
                     /locus_tag="ACHARQ_002321"
     CDS             2283..2510
                     /locus_tag="ACHARQ_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8410712.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2526..3692
                     /gene="hemW"
                     /locus_tag="ACHARQ_002322"
     CDS             2526..3692
                     /gene="hemW"
                     /locus_tag="ACHARQ_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8410713.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3927..4970
                     /gene="hrcA"
                     /locus_tag="ACHARQ_002323"
     CDS             3927..4970
                     /gene="hrcA"
                     /locus_tag="ACHARQ_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8410714.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            5064..5627
                     /gene="grpE"
                     /locus_tag="ACHARQ_002324"
     CDS             5064..5627
                     /gene="grpE"
                     /locus_tag="ACHARQ_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8410715.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5678..7507
                     /gene="dnaK"
                     /locus_tag="ACHARQ_002325"
     CDS             5678..7507
                     /gene="dnaK"
                     /locus_tag="ACHARQ_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8410716.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7658..8824
                     /gene="dnaJ"
                     /locus_tag="ACHARQ_002326"
     CDS             7658..8824
                     /gene="dnaJ"
                     /locus_tag="ACHARQ_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8410717.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3129 a         1441 c         1909 g         2453 t
ORIGIN      
        1 attttataat tggatcactc attgttttac ctaagtcata tttaagtaat aaccggtgtt
       61 tttacataag aagacttcgg gtatttttta gtttgattag ctctgatata cctataaaga
      121 atatgctaag gtaagaatag taaaaattca catagatgag gtataataat gatattttta
      181 tatattacag ataacttgac acaaaatgag tttttttcga tttttcaagt tatctaatgc
      241 aattatattg atatcattag gagctgcatt gttatgaatc caacattgat tactaagaaa
      301 gaattactaa aaaaattaga tatttctact ggtgtattag ctaatttaat tagaaatggc
      361 atgccaaaag agggagaaat gtttaatctt gataagatta ttacatggcg agaaaattgg
      421 tctaaaaaca tattaggtga gttagaagtt ggtagagtat atactaacaa agaaatttca
      481 gagaagttta aatgttccaa acagggtgga atgagaagat ctcatcaaac taatactctt
      541 gtactgttta gtgatcaaac aggaagtaat gtttataaag ataagtggtt aaatggtatt
      601 ttacaatata ccggtatggg gctaaaaggg gatcaagtat tggataagaa tcaaaataaa
      661 gttttagcca attcaaaaag taattttgta aagattcatt tatttgaaac atttaaacca
      721 aaagaacaca catatctggg agaagtatat ttagcaggac aaatatatac tgttaatgaa
      781 aaagatagtt cgggcaacag cagaaaagta tataaatttc cattagcttt aataaatcaa
      841 gagcaattga ttgaagataa agatatataa ccaggaagaa aatcaaacta gacatattcg
      901 taatttatct aatgctaaat tagaggaaga agcaagaaaa gttagtaatt ataataaaaa
      961 aatggctgca aaatatgtta acgaagagga gttttgcttc tcaaaaacaa aaatatataa
     1021 acgtaatccc ttcgtatctg aatatgttaa acgacttgca aaaggtattt gtcaactttg
     1081 ccaagaaaaa gggccattta taaaagacgg tgtaccttat ttacattgtc atcatataga
     1141 atatttgtcg caaggtggaa aagatgtgat tgaaaactgt atcgcgttat gtcccaattg
     1201 tcatgctaga attcatgaac ttgagttacc tgacgataaa gaaaaattgc tgaagatcgt
     1261 tgaaaaaaga gaaaatagct tttctaaaat accgtaattt atttttatag gacaaataag
     1321 ctaatgtgag ctatgccttc tatataagac taagttctat tataagtaac gaagtaattc
     1381 tcgttccttc ctgtagtaat attttttaaa taacctatag gcttcttcaa aagtacatcc
     1441 tccagtaaat tctaagtact ggaggattct ttttctagtg ttgctttact catgaaaaag
     1501 tagttggcta cttcatcaaa atttctgaac ttttttaatg tataataaag agctcgatca
     1561 tttactacaa ggatgctagc tcctacattt tcacagtttt ttagcaagta aatttttcta
     1621 aaatcacatc aggaaatgca tgagattctt taagagaggc aattataggg ctatgattac
     1681 ggttcgtgta agtgacgcca aaaaagaatg gctttcttat atggctgaat tttatgggat
     1741 tactttatct gaacttctaa aaaactattc gatggaacaa ctagaagatg aatacaatcg
     1801 tcaaacggct gaaattgccc ataaacattg gataagaagt gggaaaacaa cagtattcat
     1861 ggaagaatcc ttaatgaatt tggtgggctg gaatgacctg ccgtttaaaa ttcacgcccg
     1921 aaattcagaa gcagctctga ttggcaaata cgtgtattgg tagagataga agataatcaa
     1981 ttcgttgtta tcgctattca agtagggcac cgaagaaatg tctatgatta agagactgtt
     2041 tatttttaca aaaaaataat aattggaatc tagttaaaac tcttgaagtg agagatttga
     2101 ctagattttt ttatgaataa aataaggaca aaattgttta ggaagaatca tgagtgtaaa
     2161 aaaatcatcc aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt
     2221 agtatgcgta tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat
     2281 ctgtgaaaat agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg
     2341 cgattaccgt ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag
     2401 catttttaga gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc
     2461 ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa
     2521 tttcaatgat agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc
     2581 cattttgcga gcatatctgc tattactgtg actttaataa agttttttta gaagggcagc
     2641 cagtagatga gtatgtggaa atgctgctga atgaaatgag attaatgctg gaaagtcatc
     2701 ctacagcgaa tttggaaaca ctttatgtag gaggcggaac accaacttct ttatctgcta
     2761 aacaacttga tcgactatta tcaggtgcta gagagatcct gccttttcaa aatggtaatg
     2821 agtttactgt agaagcgaat cctggtgatt tgacccgtga aaaacttcaa gtgatgaaaa
     2881 actatggcgt caatcgttta tccatgggag tgcagacatt tgacaatcgc ttattgaaaa
     2941 aaattggacg taaacatact gcagaagatg tgtatcaaac aatgcaattt ttagaagcag
     3001 aaaattttac caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag
     3061 gatatcggga tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact
     3121 cattgatcct cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac
     3181 tgcctgatca ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc
     3241 atggacgtca ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata
     3301 acctcatgta ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc
     3361 ttggagatat tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag
     3421 caaatgaacg cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag
     3481 aaatgttctt aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct
     3541 atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt
     3601 tacttgttaa tgaagaagac cgtgtttatt tgacaccaag agggactttt ttggggaaca
     3661 atgtgtttga gcgcttttta tttgaaaaat agtttgaaga atatttgttt tttatcgaaa
     3721 cttgaaaaga aaaagtttag aaagtttgac aatgaggtat tgaagtattg atataactgg
     3781 atttacatca attatttaga attagcactc ttgtgtttag agtgctaaaa aatggagtct
     3841 ttctcttgac aggagaaggg cttcttgcta tatttataag tgaattagca cttagaatat
     3901 atgagtgcta atgagaggtg aggaagatgt taacaaagcg acaaaacgac attttacgtc
     3961 tgatcatcca acactatacg agtagtggcg tgcccgttgg ttccaaaaca ttaatgtctg
     4021 agggagtcaa ggccagctca gcaacgattc gcaatgatat gaaagcatta gaagatgaag
     4081 gattgctgtt gaagactcac tcttcttctg ggcggattcc atctgcttta ggctatcgtt
     4141 attacgtgga tcatttgtta aaacctgctc gcgtagcaga agatgatttg ctgctgattc
     4201 gtcagtctct aggcagagaa tttcatgaaa tcaatgagat tattaagcaa tctgctgaga
     4261 tattatctga gctgacaagc tatacgactt tttcattggg acctgaaata aaagaccgcc
     4321 gactgactgg ctttcgtatc gtacccttga ataatcgaca gattattgct atcgtcgtaa
     4381 cagatatggg gaatgtagaa agccaagtgt tcactcttcc cgaatatatg ggaagtcagg
     4441 aactagaaaa aatggtacag attgtaaacg accgtctagt cggagatcct ctgatgacgg
     4501 tgtaccataa acttcgtacg gagattccga tgattcttca taaatatttc cagacgacag
     4561 aagggatgtc tcagttattt gacaccgttc ttggtcatgt ttttgaagaa aaggtttatg
     4621 tcagtggaca aatgaatctc ttggattatg agccccgtca agatcttgat cagttcaaat
     4681 cgatgttttc ttttatgaaa gactcagacg gacttacaca aatgatcact ccgatggata
     4741 gcaatattca tatcaagatt ggtgctgaat taggcaatga tttattgcag aatatgagta
     4801 tgattcaagc aagttacgag atagatggtc atggaagagg tacaatagcc ttgttagggc
     4861 ctactagtat gccttattct cggatgttcg gtctagtcga tgtttatcga agagaactag
     4921 cagctaaact tgctgattac taccgctcgc ttgacttgtc aaactcgtaa tgtgaataac
     4981 gaagctattt gcagctgatc gttctctctt tcttagtaag agcaatcaaa cagctgactg
     5041 aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga agaactagat aaagaaatga
     5101 cagatgctca gccagaaccg gaaatcgatg tcgaagctgc agaagattcg ggtatttcag
     5161 aagctgaagc ggaagaattc gaaacagcga aattaaaagc tgagttagaa gaaatggaag
     5221 ataaatactt gcgggctcgt gctgaaatcg caaatatggc aaatcgagga aaaaatgagc
     5281 gtgagcaatt acaaaaatac cgttcacaag atttagccaa aaaattactt ccatcaattg
     5341 acaacctgga acgtgcatta gccacagaag tgtcagatga tcaaggggca ggtttgaaaa
     5401 aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact ggaagaagaa ggaattgaaa
     5461 aaatccctgc taaaggagaa gcctttgatc ctaatcttca tcaagcagta caaactgttc
     5521 cagcgactga agatacacca gccgatacga ttgtagaagt actacaagaa ggctacaaat
     5581 tacatgatcg cgtgttaaga ccaactatgg tcattgttgc acaataattg tgcggatata
     5641 aaacttacac tttcaattaa agaaggagac gttaattatg agtaaaatta tcggtattga
     5701 cttaggtacg actaactctg cagtagcagt attggaagga aacgaagcaa aaatcatcgc
     5761 taacccagaa ggaaaccgta caacaccttc tgttgtatca ttcaaaaatg gagaaatcca
     5821 agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca aatacgattt catcaatcaa
     5881 acgccatatc ggtgaagcag gttacaaagt agatgtagaa ggtaaatcat atacaccaca
     5941 agaaatctca gcaatgatcc ttcaatatat caaaggattc gcagaagact atttaggtga
     6001 aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt aatgatgcac aacgtcaagc
     6061 aacaaaagat gctggtaaga tcgctggtct agaagtagaa cgtatcgtca atgaaccaac
     6121 tgcagctgct cttgcctatg gtttagataa aacagatcgc gacgaaaaaa tcttagtatt
     6181 cgaccttggt ggcggtactt ttgacgtatc tatccttgaa ctaggagacg gtgtctttga
     6241 tgtattgtca acagctggcg acaaccatct aggtggggac gactttgata acaaaatcat
     6301 cgactacatg gtagcagaat tcaaaaaaga aaacagcatt gacttgtctc aagacaaaat
     6361 ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa aaagatttat ctggcgtatc
     6421 aagcacacaa atcagcttgc catttatcac agcaggagaa tctggaccat tgcatttgga
     6481 aatgacattg acacgtgcaa aatttgatga attaacagct gacttagtag aacgtacaaa
     6541 agtaccagta cgtcaagcgt taaaagatgc aggtctgtca caatcagaaa tcgacgaagt
     6601 tattttagtc ggtggatcta ctcgtatccc tgcagttgta gaagcagtac gtaaagaaac
     6661 aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta gttgctatgg gtgctgctat
     6721 ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt ttacttgacg ttacaccatt
     6781 gtcattaggt atcgaaacaa tgggtggagt gttcactaaa ttgattgacc gcaatacaac
     6841 gattccaaca agtaaatcac aagtattctc aactgcggca gataatcaac cagcagtaga
     6901 catccatgta ttacaaggtg aacgcccaat ggctgctgac aacaaaactc taggaagatt
     6961 ccaattgaca gatattccag cagctcctcg tggtattcca caaatcgaag taacattcga
     7021 tattgataaa aacgggattg taaatgtatc tgcgaaagat ctaggtacac aaaaagaaca
     7081 aaaaatcacg atcaaatctt cttcaggtct aacagacgaa gaaatcgaac gtatggtgaa
     7141 agatgcagaa gcaaacgctg aagcagataa agctcgtaaa gaggaagttg acctacgtaa
     7201 tgatgtagac gcattgctat tctctgtgga caaaacattg aaagaattag aaggcaaagt
     7261 ggacgaagaa gaagtgaaaa aagcagaaac agctcgtgac gaattaaaag ccgctgttga
     7321 agcaaacaac atcgaggaaa tgaaaactaa acgcgatgca ttgaacgaaa tcgttcaaaa
     7381 cttgactgtt aaactatacg aacaagctgc acaacaacaa gctcaagaaa atccagaagc
     7441 agctcaaggc ggagcagatg atgtcgttga tgctgatttt gaagaagtcg atggcgacga
     7501 taaataatca gttcactgaa tttcgagaga aggccaaagt tgctactttg gctttttctt
     7561 ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa aatatgatat gataacaagc
     7621 gacgtgaaaa tgatgagaac caataggagg aaagcctatg gcaacaaaac gtgattatta
     7681 tgaagtctta gggctctcaa aaggagcatc agaagatgaa ataaagaaag cttatcgtaa
     7741 gctttcgaaa aaataccatc ctgacatcaa taaagaacct gatgcggaag aaaaattcaa
     7801 agaagtatca gaagcatatg agattttaag tgacccgcaa aaacgcgctg catacgacca
     7861 atacgggcat gcaggcacag atccaaatta cggtgctggt ggagctggtg gcggatttgg
     7921 tggattcggc ggcttctctg gtggcggctt cggtggtttt gaagatatct tcgactcttt
     7981 ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct cgtcaaggtg ctgatttgca
     8041 atatacggtc gacttgtctt ttgaagaagc gatttttggt gtagaaaaag aaatcaagta
     8101 taatcgggaa gaaatctgtc atacttgtgg tggaaacggt gcaaaaccag gcacacaacc
     8161 aactacttgt cataaatgtc atggtgcagg tacgatcaat gtagaacgcc aaacgccact
     8221 tggacgagtg atgagccgtc aaacttgtga tgtttgtcat ggcacaggga aagaaatcaa
     8281 agaaccttgt ccaacctgtc atggctcagg acatgagaag aaagcacatt cagttaaagt
     8341 gaatgtacca gctggtgtag aagaaggaca acaaatgcgt ttagctggtc aaggagaagc
     8401 tggagaaaac ggcggaccat ttggtgattt gtatgtggtt tttcgtgtag aagaaagtga
     8461 tatctttgat cgtgaaggtt ctgaaattta ctatgaattg cctttaagtt ttgtacaagc
     8521 agctttgggg gatgaagtcc aagtaccaac tgtccatgga gatgtgaaat tgaagatccc
     8581 tgctggaaca cagacaggta cgaacttccg tttacgtgga aaaggcgctc cgaagttacg
     8641 tggtggtgca acaggcgacc aacatgtcaa agtaaaactg atcacaccga aaaatctaaa
     8701 tgaacaacaa agagaagcat tacgtgcatt cgctgaagct ggcggcatca aagttgaaga
     8761 acagcaagaa gacggtttct tcgataagtt taaagatgct tttggcggga aaaagcggaa
     8821 gtagtttttc tagtaaaaat ataaagtaga cttgcccaaa ttttctattt ctagtaaaaa
     8881 cgctaagtca ttaataatta aaaaccagac gaacaatcat acgttcgcct gg
//
LOCUS       DAYYAG010000115         8676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000115 DAYYAG010000000
VERSION     DAYYAG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.118"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..389)
                     /locus_tag="ACHARQ_002327"
     CDS             complement(<1..389)
                     /locus_tag="ACHARQ_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8410718.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQ"
     gene            complement(395..1258)
                     /locus_tag="ACHARQ_002328"
     CDS             complement(395..1258)
                     /locus_tag="ACHARQ_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8410719.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1272..2168)
                     /locus_tag="ACHARQ_002329"
     CDS             complement(1272..2168)
                     /locus_tag="ACHARQ_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8410720.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2343..3632)
                     /locus_tag="ACHARQ_002330"
     CDS             complement(2343..3632)
                     /locus_tag="ACHARQ_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8410721.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3604..5121)
                     /locus_tag="ACHARQ_002331"
     CDS             complement(3604..5121)
                     /locus_tag="ACHARQ_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8410722.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5319..6209
                     /locus_tag="ACHARQ_002332"
     CDS             5319..6209
                     /locus_tag="ACHARQ_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8410723.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6282..7106)
                     /gene="nadE"
                     /locus_tag="ACHARQ_002333"
     CDS             complement(6282..7106)
                     /gene="nadE"
                     /locus_tag="ACHARQ_002333"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8410724.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(7118..8590)
                     /locus_tag="ACHARQ_002334"
     CDS             complement(7118..8590)
                     /locus_tag="ACHARQ_002334"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8410725.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         2682 a         1794 c         1425 g         2775 t
ORIGIN      
        1 atttgtaaaa atgtgatatc tttgttttca gaaatcacat atatccagga taaaagaaac
       61 atggaataaa taagtaaaac tacatatcca ctaattgaaa gtacgattgt ctgttcttct
      121 aataattgat taacaagaaa ataaataatg atcgttaaca gactaaggaa caagttctta
      181 ccaaaatgtt gctttattcc ttgaaagaaa aaataaatga tatttcctga tccctcacct
      241 ttccattgcg ccattacata aaaaacagca catgtagcag gcacgatagt aataacaggc
      301 aaacacacga ttacccagag aatacctaat aagatgtaat cagaaaccac aatcaactgt
      361 ccataaagtt tagtctcaac tattttcatc ttcctcactc tttcattcca gacatagctg
      421 ttccttgaac aaaatatttc tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa
      481 taaccgttcc agccattaca ggtccataaa aagaaatgtt ctgccctgta aataaagcta
      541 accctgctgg taaagttctc atttttgaac tacttgtcaa tatcaatgga taaagcaagt
      601 cattccaact attcatcagc gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg
      661 gcaacatgat tttcaaaaaa atattaaact cacttaatcc atcaatgcgt gctgcctctt
      721 ccaaagaatc tggtaaagta ataaaaaagg aacgcatcat gaagatacca aatgcagttg
      781 tcatacgagg aatgatcaaa cctgcataag tatcaagcat gccaagcaaa ttgacttcaa
      841 taaatagagg aatcatgaag acttggaatg ggatcatcat tgtcagcaaa acgaaataga
      901 acaaaactga ttttcctttg aaacgcatac gagcaaatgc ataccccgca agagaatcaa
      961 agaatacaga agtgatcact gtccctccag caaaaatcac tgtatttttg atgtaatcta
     1021 ataaagggat cgttgtccaa accttaatat agttatctag ggtatagcta tctgcaaata
     1081 ttctaggcgg aaatgagata atgtcttttt cgtgtttaaa agaagaaata atcagccaca
     1141 aaaaaggaaa cgcaataacc aacaatataa ctagcatact aatcgttcga atgatttttt
     1201 taccactaac tgcttttcga ctattcacag taactgtaat cgagctttct ggtgccatac
     1261 aggtttccct cctatttcat ttgtttttct ttccgtgaca tgtacacatt agtggagata
     1321 gctatagttg caacaatgat aaacaaaacc gaagacagtg ctgatgcata gcccaattca
     1381 taaggtgctg taaatcctcg actgtaaata tattgaacag ctgtttctgt cttaaattgt
     1441 gggccaccag cggttgcaac atatacttgg tcaaacactt gcatcgaacc aatcagattc
     1501 gttaaaatac aaaaacctaa ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc
     1561 tgtcgatttg ttgcaccatc catttgagca gattcataca aagagggaga gattccctga
     1621 atagcagtca agagaatcgt catagtaaca ccaaaattct tccaaacagt catgatggct
     1681 accgtcggca tagcaagggt tggatcgcga aaaaattgag ggatcggcaa gccgacttgt
     1741 gaaaacaagt aaggaatcgc ccctacattc ggatcaagca acatagaaaa aataatcgca
     1801 attgacgtca atgaacaaat aactggaatg taaaatgtcg aacgacaaaa acgattgaat
     1861 ctggtatttt tagataatgc tactgctgct aataaaccaa cgataatttg tgttggcgtt
     1921 tcgagtacgg tgaaatataa agaatgaatc aacgaattaa ctgctcgttc atcactaaat
     1981 atacgtgtga aattttcaaa acttacaaaa tttcttgatg taaagaaaat attcaaatct
     2041 gtaaaactaa tagcaaatgt tcctaaaagc ggtatgacag taaataataa taaaactaaa
     2101 aaagatggag ttaaaaacaa atacgcagca cgatcaggac gattccaata tttggtgaat
     2161 ccgttcatat agtccacctc atctttcatt ttgataaaat atacaatctt ggtttaaacg
     2221 aatctgaata agatatccga acaatcgctg tgatttttgc aataacacct atctgcaaat
     2281 actaggtcga tcattcagat atctgtatct tgccaacagt ctatcctgta taaagttctg
     2341 tactattttt gcagtaattg attgatggca tcatctgcat cgttcatgag tgtctcagga
     2401 tcttctcctg ctaataattt ttcaatcagt ggattgatga tatcgttatt gatcgcagag
     2461 atttgggtaa aacctggtaa atatggctct gcatagtcca tttgtttcga taattcagat
     2521 acaataggat cattttttac ttcttcatct tttgctactg aatccaagta ggcaggaaaa
     2581 ccattttcga tactccattt tttaccaatt tcagttgtat tccaatattt caaaaactca
     2641 tagacagctt cttttttatt attatctgta cttgccggaa tggcatatcc tacgccatct
     2701 aaaatagctg tttttttgtc tgaatcactc ccttgtggta aggtagtcac attataatca
     2761 atctcgtttg ctttcaatcc gttatttaac catggcccat tgatttcaat ggctagttga
     2821 ccagcattca ttaaattgtc catttcggcg ccagaaatcg attctggacc agtcttgtct
     2881 acatggatca ttttttggat cgcttttaac gttttaaggt tctcaggaga cgcaaattca
     2941 gacttagtat aatcatcgtt cactaattta ccgccatttg ctaacatcca gttatataac
     3001 ggtgcagttc catccttatt gaagacaaaa cctgatacat ttttactact atcagttaat
     3061 tttggtgcca tttctgtcaa ttcttcccaa gtagtaggtg gttgttcagt atctaatcca
     3121 gcttgtttaa acaaatcatt attccaatac atatacatgc cttgtacctg catggggaca
     3181 aaatactgtt taccttctac ttcacctaat tcaatagccg tttctttaaa atcagattta
     3241 tctactccat catattccca gaaatcgtct aatggttgta ccgctccgtt ttctacatac
     3301 tgtgcaaaat ctggataatt taatgcaata aagtcaggtg cttttttaga agaaatcgct
     3361 gtaggaagtt tttcattcaa attggcccaa gtcatgatat ccatttcaat tttgatgttt
     3421 ttatcatttg ttttattaaa ttcattaacg atttcttgca atatttcacc atcagatgca
     3481 gtaaacccat tccaaaaagt caaagtcact tctttatcag aatcagattc tgctgaactt
     3541 ccgccaccgc aagctcctaa aatacctgtt gcagctacca acaaagagcc aattaaccat
     3601 tttttacctt tcattttttg ttcctccttc atctgataaa cgacatctga ccacattcca
     3661 agatagcggt ttcaataaac aattgatttt cccatcttca gcttttacat ttgttgaatg
     3721 acttggtttt acttgttcac ttttaggaag attagttgct tttgtatcaa atccactcat
     3781 ttcagaaaaa tcaatcactc cctctaatga taattcagac aagtgaacag ttagattcat
     3841 ttcttcttct ccacggttaa ctgcaaatat gacaagttct ttgtcattta ttacggctat
     3901 actttctaaa tacggcactt cttcaaaatc ttccgtcgca tagtttgctg aacgaatttg
     3961 tgggcttaac acctctcctt gtccatacac agcagcttgc ataaatggat aatagatcgt
     4021 ctgtctccaa gcttctccgt ttgtttctgt cctgattggt gcaataacat ttaccaattg
     4081 agcaagacaa gcaatcttta ctctatctgc atgtttcaat aaagtgatta atagacagcc
     4141 taatagtaat gcatcttcaa aattatagtt atcttctagt aaaggaggag caatctgcca
     4201 aggcgctgct tgctcatctt gttccgctga atgataccaa atattccatt catcaaagga
     4261 caggttgatc tgcttcttac tatgtttctt cgccttgact gtatcgcaca tcgctgtcac
     4321 ttcagaaatg aaccgatcca tgtctaagga acgtgcaaga taatgagaaa gatcattttt
     4381 ttggttgcca taatattgat gcagagacaa ataatctatt tgatcgtacg cttgatcaag
     4441 aactgttaac tcccaatcac caaatgtagg catttgccga ttcgaacttc cgcataaaac
     4501 cacctcaatt gaatcatcta cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc
     4561 atattcttcc gctgttttat gtccaatctg ccaaggaccg tccatttcat tccctaaaca
     4621 ccatgtctta attccaaaag ggctctcttg accattcgaa cgccttaaat cactccaata
     4681 agttccttta ggaaaattac aatattcgac taaattacga gctgcatcta ctccacgagt
     4741 acccagattg acagccatat tgatttcggc ccccacatgc tgacaccagt tataaaattc
     4801 atgaatccca acttcattcg tttcaattgt ccgccaagct aaatccaacc ttctaggtct
     4861 atttgctatg ggcccaatac tgtcttccca acgaaatcct gacacaaaat ttccaccagg
     4921 atatctaatc atgggaatgt tcaattcttt taccaagtga atgacgtctt ggcgaaatcc
     4981 acttttatta gctgatggat gatctggctg ataaatccct tcatatactg ctcgtcccat
     5041 atgttcaata aatgaactgt acaaccgcgg atcaactttg cctacacgct tgacactact
     5101 aatcaacatt gtggcttcca taactgacct cctttaattt atacatgaat aatacacctc
     5161 gttgaaagcg ttgtcaataa tataaatgtt aaacttactt attttttatc gattttaatt
     5221 aaccaaaata ttaataatga ttaaaacaaa taatttaata taacttttat taacaaatat
     5281 gttaatatat aagaaaaacc ttttaagagg tgatatatat gcaactacaa ataaacgaag
     5341 aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc atccagctac
     5401 tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg agcagtgcta
     5461 tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca gaaaaagtag
     5521 gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tatcagtttt ccagaaaaaa
     5581 tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat acaaattatg
     5641 caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa gtggatgaac
     5701 cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt accagcggat
     5761 ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag atgttggaag
     5821 tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct tcagatatca
     5881 ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat tttgctgata
     5941 tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac ggcttgctga
     6001 agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg tcaaacatca
     6061 cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg
     6121 atgccaaaca tgtggggggc tgtactttat ttggtaaagg attcggtaat tacgatcagg
     6181 atatcaaatt aaaagtttac tacagctgat atttttcttt tctattttat aaaacaaaaa
     6241 cagccaatcc ctatgaaggg acggctgttt ttgctttatt gttatttcca gaaatcatcc
     6301 aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt
     6361 tcttgtgctt gagcgggaac ttcttttcct tctagataat catcaatttc attatatgtg
     6421 acacctagtg cgacttcatc tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga
     6481 atttttgtgt ataatttttc cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt
     6541 ttgtccaaac ggaataatgg caaaatgtcg gctccaccat caccaaattt cgtgaagaat
     6601 ccagtaatgt tttctgcagc atgatctgtc cctaatacgg ctcctgctct ttctccagct
     6661 acggcatatt gcgtaatcat acgttgacgt gctttgatat ttcctttgtt aaaatcgctg
     6721 attcccacac ctgcttcgct taatacttgt acttgtgcat caacagctgg tttgatattt
     6781 actcgcagac taacatcggg ctggataaag tcaagagcag cttttgcatc ttcttcatct
     6841 gcttgttcac cataaggaag acgaacagcg ataaattgat agttttcatc ttttgtttct
     6901 tcacgcattt cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga atcttggccg
     6961 ccgctgatac ctagtacaaa agttttaaga aacggatatt ttttcaaata tgctttcata
     7021 aagtcaatac ttttacgtat ttcttctttt ggatcaattg ttggtttgac acctaattca
     7081 ttgataattt ctgtctgcag gctcatttat tctcctccta tgcttctgta tgaagttctg
     7141 ctacgctttt tttcattcgt tccatgatag ccattttatg gttccagcat tcagtagaca
     7201 gatcgactgg gtatttttgc ggattcaagt ctcgtttata ctcttcccat agtgaatcta
     7261 gattttcttt tgtatattct ttgatttcgt ttagtgcagg taattcgtaa actctttttc
     7321 cttcgataaa gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca gttttattga
     7381 taaacgtatg cactggatgg aacatgaaga tagcttcttc ttctcgaggg tcttcatccc
     7441 ataatgtcac gtagtctcct tcagatttcc catcaaaatt tcgagtgatg cgccacactt
     7501 gttttttccc tggagtagtt actttttctg cattgcttga taacttgatc gtatccatca
     7561 tttttccttc atcatcttcg atcgatacta gcttgaatac tgcaccaagg gctggctgat
     7621 cataggctgt gatcaatttt gtcccaacac cccatacatc aattttagct ttttgcatct
     7681 ttaaattcaa aatcgttgct tcatccaagt cattcgaagc atagattttt gcttccgtaa
     7741 atccggctgc atctaattgt tcccgaacac gcttagatat ataggccata tctccactgt
     7801 cgatgcgtac gccttggaag ttgattttgt cacctaattc tttcgccacg cgaatcgcac
     7861 ttggcacacc tgatttcaac gtatcataag tgtcgactaa gaacacgcag tctttatggg
     7921 ttttagcgta ggccataaat gcttcataat catttccata agattggact aacgaatgag
     7981 catgggtccc gctagctggt atcccaaata tcttcccagc acgtacattg cttgttgcat
     8041 ctgctccgcc gatatatgcc gctctagttc cccagactgc tgcatccagt tcttgtgccc
     8101 gtctagttcc aaattccaat agtggatctt ctccgatgac agatttgata cgagctgctt
     8161 ttgtcgcgat caacgtttgg aaattgacca tattcaacag tgctgtttct actaattggc
     8221 agtgcgctaa aggcccttcc acttggatta atggttcatt tgcaaaaact agttctcctt
     8281 cacgagcaga acgaactgtt gctttaaatt caaaattttg taaatactcc aaaaaacctt
     8341 caggatagac ttctaatcca cgcaagtaag caatatcgga atcattgaac gtcagattct
     8401 ctaaataatt gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca
     8461 tttctcgaaa gtaacattca aaaacagcat gtaaatcagc tcgacctaat tcccagtatg
     8521 tctgcatcat attgatttga tatagatccg tatgcagcgt caaactgtcg tctgggtaag
     8581 tattattcat cttgtattac tcctcttggc aagttttcat ttcctaccca ttataacaaa
     8641 aaaaattaat aggaaaagaa atcttccatt ttttct
//
LOCUS       DAYYAG010000116         8531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000116 DAYYAG010000000
VERSION     DAYYAG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.119"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..351
                     /locus_tag="ACHARQ_002335"
     CDS             64..351
                     /locus_tag="ACHARQ_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410726.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            767..2305
                     /locus_tag="ACHARQ_002336"
     CDS             767..2305
                     /locus_tag="ACHARQ_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8410727.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2302..3267
                     /locus_tag="ACHARQ_002337"
     CDS             2302..3267
                     /locus_tag="ACHARQ_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410728.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3254..4576
                     /locus_tag="ACHARQ_002338"
     CDS             3254..4576
                     /locus_tag="ACHARQ_002338"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8410729.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4639..4743
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4817..5560
                     /locus_tag="ACHARQ_002339"
     CDS             4817..5560
                     /locus_tag="ACHARQ_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8410730.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5575..6567
                     /locus_tag="ACHARQ_002340"
     CDS             5575..6567
                     /locus_tag="ACHARQ_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8410731.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6579..7316
                     /locus_tag="ACHARQ_002341"
     CDS             6579..7316
                     /locus_tag="ACHARQ_002341"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410732.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7306..8028
                     /locus_tag="ACHARQ_002342"
     CDS             7306..8028
                     /locus_tag="ACHARQ_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8410733.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8112..>8531)
                     /locus_tag="ACHARQ_002343"
     CDS             complement(8112..>8531)
                     /locus_tag="ACHARQ_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8410734.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2758 a         1463 c         1838 g         2472 t
ORIGIN      
        1 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
       61 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      121 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      181 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
      241 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
      301 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
      361 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
      421 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
      481 tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata
      541 gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga
      601 accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt
      661 atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa
      721 aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat
      781 acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat
      841 ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc
      901 caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc
      961 ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt
     1021 gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc
     1081 tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg
     1141 cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc
     1201 cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg
     1261 gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat
     1321 cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa
     1381 acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt
     1441 atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga
     1501 acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt
     1561 tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca
     1621 aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga
     1681 agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt
     1741 gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt
     1801 ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc
     1861 tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata
     1921 taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag
     1981 tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga
     2041 tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc
     2101 ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa
     2161 agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca
     2221 agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg
     2281 attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag
     2341 tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt
     2401 gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta
     2461 tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg
     2521 cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg
     2581 acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga
     2641 gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat
     2701 cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt
     2761 gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca
     2821 agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt
     2881 ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag
     2941 gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac
     3001 tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta
     3061 gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt
     3121 gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta
     3181 gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga
     3241 gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt
     3301 aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct
     3361 atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt
     3421 agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc
     3481 caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg
     3541 aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga
     3601 tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac
     3661 cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga
     3721 ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc
     3781 ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa
     3841 tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga
     3901 agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac
     3961 tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac
     4021 tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa
     4081 acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg
     4141 agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta
     4201 tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac
     4261 gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc
     4321 gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga
     4381 accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt
     4441 gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt
     4501 gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc
     4561 ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc
     4621 tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt
     4681 gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac
     4741 gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa
     4801 gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt
     4861 aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc
     4921 tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac
     4981 gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc
     5041 cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc
     5101 acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat
     5161 gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc
     5221 ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga
     5281 tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg
     5341 tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc
     5401 tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt
     5461 ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt
     5521 attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa
     5581 aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa
     5641 acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta
     5701 ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct
     5761 ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt
     5821 gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat
     5881 ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc
     5941 gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca
     6001 ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg
     6061 gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa
     6121 tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt
     6181 attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg
     6241 gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg
     6301 gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg
     6361 ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg
     6421 tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc
     6481 aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa
     6541 ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact
     6601 tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag
     6661 atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt
     6721 taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg
     6781 gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg
     6841 tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag
     6901 taagggatta tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat
     6961 tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt
     7021 acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag
     7081 actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata
     7141 tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg
     7201 tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat
     7261 cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct
     7321 cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg
     7381 acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt
     7441 aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa
     7501 caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt
     7561 tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca
     7621 gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat
     7681 tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa
     7741 caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca
     7801 gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga aacggatgga
     7861 ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg
     7921 cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa
     7981 aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg
     8041 acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta
     8101 ataaatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa
     8161 ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca
     8221 ccatttaagt aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg
     8281 taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga
     8341 gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt
     8401 ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga
     8461 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
     8521 tgtcctgagt a
//
LOCUS       DAYYAG010000117         8525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000117 DAYYAG010000000
VERSION     DAYYAG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.120"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..795
                     /locus_tag="ACHARQ_002344"
     CDS             16..795
                     /locus_tag="ACHARQ_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410735.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            841..1785
                     /locus_tag="ACHARQ_002345"
     CDS             841..1785
                     /locus_tag="ACHARQ_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8410736.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1801..2580
                     /locus_tag="ACHARQ_002346"
     CDS             1801..2580
                     /locus_tag="ACHARQ_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8410737.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            2592..3290
                     /locus_tag="ACHARQ_002347"
     CDS             2592..3290
                     /locus_tag="ACHARQ_002347"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8410738.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            3318..4082
                     /locus_tag="ACHARQ_002348"
     CDS             3318..4082
                     /locus_tag="ACHARQ_002348"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8410739.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4095..5912
                     /locus_tag="ACHARQ_002349"
     CDS             4095..5912
                     /locus_tag="ACHARQ_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8410740.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            5923..6555
                     /locus_tag="ACHARQ_002350"
     CDS             5923..6555
                     /locus_tag="ACHARQ_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8410741.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            6557..7675
                     /locus_tag="ACHARQ_002351"
     CDS             6557..7675
                     /locus_tag="ACHARQ_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8410742.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            7668..8297
                     /locus_tag="ACHARQ_002352"
     CDS             7668..8297
                     /locus_tag="ACHARQ_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8410743.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            8290..>8525
                     /locus_tag="ACHARQ_002353"
     CDS             8290..>8525
                     /locus_tag="ACHARQ_002353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410744.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILS"
BASE COUNT         3016 a         1457 c         1775 g         2277 t
ORIGIN      
        1 cttaggagtg aatctatgga aaagccattc gtcaagtcat tgactgctgt ggcggtaggt
       61 gcaggtgcta ttgcgatctg tttgtttggt taccatatca ataatcagcg gcaacatcag
      121 caacggatca attatgcaga atctgccatc accaatcaaa aagacacctt gacttcttta
      181 tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa attttctaaa ccctgaaata
      241 acagaagaaa caatcacaag tttgtcaaat aagctatcct ctgtcaaact ttcagcagat
      301 gattacgaca tcaaagaatc agaattgcct aaagaagctg ttgcaatcca agatgagaag
      361 aaagcaatcg tgacacagtt agatagtgca gaatcaaaac tgaagatcca gacagctgtc
      421 aacgatttat ttactaaaaa tgtatcaaac tggcagcaag ctgtagacga tgtcgtcatc
      481 aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg aaaatatgag cttttttaaa
      541 gatagtcctt ggaaaacggt tgttatgcaa tatctaggat tcgctgatac tcagatcgcg
      601 caagtcacgc aactagatca gttattcgat acgatgttga aggatggtca agtgactgcc
      661 accgctactt acgatcaata tttgacggcg ctcagtcaaa tagagcagat ccggaatgaa
      721 aaaataagcg cagcatacgc aacaaaagca caaactgttg ctcagcaaat gggctatagc
      781 aatataagtt attgacagtt aaaacgttga ttcaagcaag gttagcaaac ggaggaaact
      841 atggcaagac gaagaagaaa atcaagaggg aaaaaaatat ttctgattat tttagcagtt
      901 cttatcgtat tagtattagc aattgtagga gtaggagcaa aattatatct ggatctctca
      961 cattctattg agaaaactta cgaatcagtg gaacgaaccc aaggagaacg aacgaaacca
     1021 gttgatctgg acaaacaaga atctttctct gttctattga tgggcattga tactggagat
     1081 ttaggtcggg tcgatcaagg aagatcagat acgatgatgg ttgccacagt cagtcctgaa
     1141 gacaaacaga caacgatcgt cagcattcct cgagatacgt atgtcgacat cgtaggtcgt
     1201 gacacaaaag acaagatcaa ccacgcttac gcattcggcg gagctgccat ggcgatggat
     1261 accctacaaa aatatgtaga catcccaatc gatcactatg tctctatcaa tatgaaaggg
     1321 ctaaaggaac tcgttgatgc agttggtggt atcgaagtca ataatgacct cactttctca
     1381 caagatggct atgactttcc aatcggtaaa attacattga acggggatca ggcattatct
     1441 tattctcgta tgcgttatga agatccaaat ggtgactatg gtagacaaga gcgtcaaaga
     1501 aagatcgtag aaggtgtagc aaaaaaagta ttgagtttaa acggtgtcag caactatcaa
     1561 tccgttttga atgctctaga gtcgaatatg aaaacagaca tgagctttga tgaaatgaaa
     1621 aaaatcgctt tagattatcg ggaggcattt ggtacgatca agcaagatca gctgcaagga
     1681 gaaggcttca tgcaagataa tatctcctat caaaaagtca gcgatcaaga acttgatcgt
     1741 gtgaaaaaag aattaaaaga tcaaatgaat ctagagaata aataggaaag tggagaaaaa
     1801 atggaagaga caatcagctt gaaagaaatt tttgaaacat tacgcaaaca cttggtaaca
     1861 atcatcatct caatgtttgc tggcttggcc atctcaggaa tcgtaacatt tttcatcttg
     1921 acaccaaaat acagttcaca agcacaattg atcgtgacac tgccacaatc agacacagca
     1981 aatgtcaatg atgtcaatac gaacctgcaa atgatcaata cgtataaaga tatgatcgtc
     2041 agtgatttag tactaaatga agtcaaagat cgtttagaaa cagaagacaa tgtgaaaatg
     2101 actgctggac aaatcaaaga tgcgatctcc gtcaatcagt cacaaaattc acaaatgttc
     2161 tcgatccagg caataagcac gaatgcagtg actgctcaac agatcgctaa tacaacttca
     2221 caagttttcc aagaaaacgc caaagacgtg atgaatgtcg ataaaatctc gattatttca
     2281 ggtgcagttg ccaatacgac acctgtttcg ccaaataata aattgaatct agtgattggc
     2341 ttagtgttag gaatgatggt aggcgtaggt ctggcattct tattcgaatt acttgatcgg
     2401 acagtgaaag atgaaaaata tgtgacggat acattaggat tcccaatctt aggaacagtt
     2461 ccagaaatgt cagcaaaaga attgaacgct ggtatccgta aaaaaggatc agctcctgca
     2521 cgaccaaaag ctacaaacga taaaacagcc ccatcacgca gaagccgctc aagagtctag
     2581 gaggaagaaa aatggcacga acacagaaac aaaaagcaac tcaaactcat gcagtcagtt
     2641 tgatcacatt aacaaaccca tcttcaccaa tcgccgaaca atatcgtacg atccggacga
     2701 atatccagtt tgcctccgca gctggtcagc aaatcaaaac gattgtcgtg acttcatcag
     2761 gtcctggaga aggaaaatcc accactgcag caaacattgc cgttgttttt gccaaatctg
     2821 gtcaacgggt actgctagtc gatgcagact tacgtaagcc cgtcatctat aaaacgttca
     2881 agttgaacaa cgcaagcgga ttaagtactg ttcttagttc atcaggaagt gtagcagatg
     2941 agatccaacg aacaccagtc gacaatttgt cgattctgcc aagtggacct aaaccaccaa
     3001 atccatcaga actactaagt tcgccaagaa tggatcagat tttagcagaa gcaagacaac
     3061 tatttgatgt agtgattttc gacatgccac cagtcgttgc cgtaacagat gcacagatca
     3121 tgtcttcaaa aacggatggc accttattgg tcgtacggga aaatacgtca cgaaaagaat
     3181 cattgactaa agcaaaagaa ctgttagata tggtacaagc gcgagtcctt ggtgttgtat
     3241 acaatggagc agaacacagc agagatgctg gctattacta cgggaattaa gaaataaaaa
     3301 gagaggagac aagagaaatg atcgatctac attgccatat cctgccagga atcgatgacg
     3361 gagcagaaaa catagatgcc agtatcgcca tggctgaaaa agcgattcaa caagggatca
     3421 cacatatttt atgcacaccc catcacaata atggaaaata cagcaatgaa aaatcacaag
     3481 tcatttctct agtcgcttcg ttacaagcag aattagagaa aagacaattg cctttgacgt
     3541 tattggaagg acaagaagta cgtattaccg gaacactgat tgaagacatc caccgagatg
     3601 aaattttatt tacagattta gatgatacat atcttctaat cgaatttccg acactggaag
     3661 tgccgttata tgcagaatgg ctcttttttg agttgttaca actaggaaag acaccagtga
     3721 tcgtccatcc agaacgcaac gcgcattttc gagaagaccc aaatcatttg ctgccattcc
     3781 tagatatggg atgcttggct caactcaccg caccaagtta tgtcggtgta tttggaaaag
     3841 acatccagaa aacagccaaa atgatggtca aacataacct cgtgcaaatg gctgcttccg
     3901 atgcccatgg ggtctctaaa cgtacctttt acttaaaaga atgttatgag caaatcgcca
     3961 aggactttgg taaggaaaaa gtagcacaga tgaaacaagt cgcaaaggac ctgatcaacg
     4021 gggatgagat cagttatcca gtctatgaag aggtaaaaaa gaagaagttt ggtttatttt
     4081 agggggaatg tagggtgttt gtattaacaa gaagaagaaa aattgccaca ttggtatttg
     4141 tggacagctt gttgttgatt gctgcgaata ttgcagcagc taaattcatg aagccgtttg
     4201 tagctatacc aatggacctt attttaatct ctatcggttt gtcgattggt ttttatttat
     4261 tctatggtag tttattcaaa gtttttactc gaatcaatcg ttatacaaat ttaagagaga
     4321 tcattgcgat ttttggttcg ttatctgcaa gtgcggcctc aagtatcatc atattgcttt
     4381 tcataaataa acaatacagt ttacgtttag tgatctttgc atacctactt tcgctattac
     4441 tgatcattgg cagccgattg atctggcgta tctatatcga aacaaaaaat atgaggtatc
     4501 tatcaacaga ttccgctaaa aataccttga tcgttggggc cggcgaaggt ggacgtattt
     4561 tatacaacag ctttcttggt tctaaaacgg ctcaagatat ccatgtcatt ggattcgtgg
     4621 atgatgatcc gaataaaaga aatacctatc tttcaggaaa aaaagtactg gggtcattaa
     4681 aagatatacc ggaattgatc gaaaaatatg acatccaaat ggtaacgatc gctatccctt
     4741 ctttatcaag aaaaaaatta cgaagaatct tcgagttagt cgaatctgct catgtgaaag
     4801 tcaatacgat gccttctatg gaagaacttg cctcagggaa aatcagcgta tcaaaattaa
     4861 aaaccatcga cgtagtggaa ttactgggaa gagatgaggt agagttggat atcgaatcca
     4921 tcaaagacca gatcacaaac aaagtcattc ttgtcaccgg tgcaggagga tcaatcggtt
     4981 cagaaatctg ccggcaaatc atccaatttg accctgcaaa actattactt ttaggtcatg
     5041 gggagaactc gatttacttg attgaccgtg aattacgcac gcatcatcaa aagtgtccta
     5101 cagaaatcgt gccgatcatt gcggatatcc aagatcgcaa gaaaatcaat gagatcatgg
     5161 aacaatacca cccagatatc gtctaccatg cagccgctca taagcatgta ccattgatgg
     5221 aatataaccc aaaagaagca gtcaaaaaca atattttcgg tacaaaaaat gtagcagaag
     5281 cagccaaagc agcgaaagta aaaaacttcg tcatggtctc gacagataaa gccaacaatc
     5341 cgccaaacgt catgggttca acaaaacgaa tcgctgaaat gatcgtgact gggttgaatg
     5401 aagaaggatg tacgaaattc tcagctgtca gatttgggaa tgtccttggt tctcgcggta
     5461 gtgtgattcc agtattccgg gaacaaattg ctcaaggagg gccagtcact gtaacagact
     5521 ttcgtatgac ccgatatttc atgacgattc ctgaagcaag tcgtttagtt attcaatctg
     5581 gggcgttagc aaaaggcggc gaaatcttta ttttagatat gagtgaacca gtgaaaatcg
     5641 tagatctagc aaaaaacatg atccgattga gcggatatag cgaagacgag atcgaaatca
     5701 ttgaaacagg tatccgtcca ggggaaaaac tatatgaaga actattgtta gataaggaaa
     5761 gaaacgatga agcagtttac gagaagatct ttgtgggaaa catcaaaggt tattccatgc
     5821 aaacagtgat ggaatttgtc aagagcctgc cgcaagacga tgaacaattg gctaaagaca
     5881 ttgtcacttt tgcgaatgct tcgaataagt agggggagta aagtgaaagc aattattgta
     5941 ggagctggtg gatttggcaa agagattgct tttctactag aaagtttctc tagatatgaa
     6001 ttgattggtt ttgtcgatga ttcgttaaaa atgcaaaatc aagaattagt gggaaaaact
     6061 gtattgggaa caatagactc gttattagaa ttagaagaag aaactgctat ttttctggga
     6121 atcgcttcgc cagatatcaa agagaaaata tatcaaaaga taaataaaaa taataaattg
     6181 attttcccta atttagttgc atcaagtgca ctagtgggca taaatgttca gttaggaatt
     6241 ggcaatattt tgatgcccta tacgacttac actgctgacg tagttttagg aaatttcaat
     6301 atgatcaata tcggctctac tatcggacat gatactcaaa tcggtaatta taattcaatt
     6361 tttccaagtg taaatatttc aggacatgtt ctattaggag ataaaaatga aattggagta
     6421 ggtactaaaa taatacaaaa tctaagaatt ggaaatatga acattatagg tgcgggaagt
     6481 gtagtaataa aaaatattaa taataagact aaaaatgttg gcgtacctac caaagtcata
     6541 gaaagatggg attaagttgg aaaataaacg aatattatta gcatctcctc atatgagtga
     6601 tgaaggatat gaacaagagt atgtaaaaga agcatttgat acaaattggg tggctccttt
     6661 gggtatgaat gtcaatggtt ttgaagagga attagcagaa tatgtcggtg ctagtcatgc
     6721 agctgcacta tcttctggaa cagcagctat acatctagct ttaaaagcgg ctggggtcgg
     6781 taaagatgat atagtgattt gtcagtcttt gacgttctca gctactgcca atccaatcat
     6841 ctatcaagga gctattccag tatttgttga tagtgattac gaaacttgga atatgaaccc
     6901 tgaacatttg cgaacagcgc tagaaaaata tcctaacgca aaagctgtat tagtagtgca
     6961 tttgtacggt ttatcagctg aactagatga aattactaaa atatgcaaag aacataatgt
     7021 tgttttaatt gaagatgctg cagaatcatt aggaacaaca tataaaggaa aatatactgg
     7081 tacattcggc gactatggaa tttattcttt taatggaaat aagataatca cgacttcagg
     7141 tggcggaatg cttgtgtcta atgacaaaga aaaggtggaa aaagtgaggt tttgggctac
     7201 gcaatctcgt gagaaggcac gccattatca acacaatgaa attggctata attatcgaat
     7261 gagcaatatc gttgcaggta tcggtcgagg acaattgaaa gttttaaatg aaagaattga
     7321 gaaaaaacga tatattcata gtttttataa aaaagaattg tctaaattgt caaatataca
     7381 attcatgcca gaaaataatt ggaatgaacc aaattattgg ttaagtgtca taacagtaaa
     7441 gggaataaat acgaatcatc ttattgatga gttagataaa aataatatag aagcccgccc
     7501 gatatggaaa ccaatgcatt tacagccagt tttcgataag tatgagtata ttggtacaga
     7561 tgttgctcag acattgtttg aaaaaggtat ctgtttacct tcagattcta aaatgaccga
     7621 tgatgatttg tatcgagtaa ctaatacaat taagcgagtg atgcaaaatg tctaatagag
     7681 gtatctacga aaagttcttt aaacgtttga tagatattct tttttcaatt ttaggtttaa
     7741 taatgttatg gccactgatg acgtttgttg caatcctagt tcgaataaag cttggatcgc
     7801 cagtgatctt taaacaacaa cgacctggaa aacatgaaaa gatttttctg atgtataaat
     7861 ttcgaacaat gacagatcaa agagatgaag aaggtaattt attatcagac gatatcagat
     7921 tgactcaatt tggtaaaata ttgcgttcga ctagtcttga tgaactacca gaattatgga
     7981 atattttaaa aggagatatg agtattattg gaccgcgtcc tctgttagtt gagtatcttt
     8041 cactatacag taatgaacaa cgaaaaagac attttgtgcg cccaggttta accggattag
     8101 ctcaagtcag tggtcgtaat gcaattagtt ggaaagaaaa atttgaaaaa gatattgaat
     8161 atgtggagaa catttcaatg atgaaagaca taaaaatatt ttttttaacg ctaaagaaag
     8221 ttattgtccg tgatggaata acatcagtag caagtgggac tactgagaaa tttgagggtg
     8281 cgaagataga tgagtgagaa taaaaaaaag tggctgctta ttcatccttt tgattatatc
     8341 agtcgaacga atatattaga ggaaattctg acggaaaaca gtatcgaata ttttaatgtt
     8401 gtatctgatt actcacatat aagaaaacaa tttctatcca gtaaagaaag gcatatgatt
     8461 aaaaatcgtc atgtgataaa aacgatcgga tataaaaaaa atatatccgt aatgcgaatt
     8521 ttatc
//
LOCUS       DAYYAG010000118         8418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000118 DAYYAG010000000
VERSION     DAYYAG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.121"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1178
                     /locus_tag="ACHARQ_002354"
     CDS             24..1178
                     /locus_tag="ACHARQ_002354"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8410745.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="ACHARQ_002355"
     CDS             complement(1200..1952)
                     /locus_tag="ACHARQ_002355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8410746.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="ACHARQ_002356"
     CDS             complement(2316..4982)
                     /locus_tag="ACHARQ_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8410747.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="ACHARQ_002357"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="ACHARQ_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8410748.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7103..7918)
                     /gene="thiD"
                     /locus_tag="ACHARQ_002358"
     CDS             complement(7103..7918)
                     /gene="thiD"
                     /locus_tag="ACHARQ_002358"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8410749.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8212..>8418
                     /locus_tag="ACHARQ_002359"
     CDS             8212..>8418
                     /locus_tag="ACHARQ_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410750.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHK"
BASE COUNT         2492 a         1736 c         1495 g         2695 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acagcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
     6901 caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag
     6961 gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac
     7021 caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
     7081 actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga ttgatggggc
     7141 catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag tcttttgcca
     7201 atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata catgcagata
     7261 gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt aaccaaaatg
     7321 attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca tttttagcat
     7381 gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt gctgcttgca
     7441 caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca taaaaattag
     7501 gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa gcagattctt
     7561 ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat ggtccaaaat
     7621 catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct agcatacctg
     7681 ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg tcgataaaat
     7741 ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca catgcggtca
     7801 tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct tgcatccctg
     7861 cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga tcttccatca
     7921 aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt ttctatcata
     7981 tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa tcaaatttat
     8041 ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat tcgctattct
     8101 aataataaat aaccactatt tgatttatat atatttcagt atccttcttg ttttcatata
     8161 accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa aatgaaactt
     8221 gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat agatggctat
     8281 acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa cacagaaaaa
     8341 ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat gtttactgaa
     8401 aaaaaagaat ttcataaa
//
LOCUS       DAYYAG010000119         8262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000119 DAYYAG010000000
VERSION     DAYYAG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.122"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..629
                     /locus_tag="ACHARQ_002360"
     CDS             147..629
                     /locus_tag="ACHARQ_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410751.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            626..1288
                     /locus_tag="ACHARQ_002361"
     CDS             626..1288
                     /locus_tag="ACHARQ_002361"
                     /inference="COORDINATES: protein motif:HMM:NF019467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8410752.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            1275..1643
                     /locus_tag="ACHARQ_002362"
     CDS             1275..1643
                     /locus_tag="ACHARQ_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8410753.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            1724..2170
                     /locus_tag="ACHARQ_002363"
     CDS             1724..2170
                     /locus_tag="ACHARQ_002363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410754.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRITEQAIEKQKDKEKKEIMSIYLSSGASFFHEID
                     SMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            2879..3478
                     /locus_tag="ACHARQ_002364"
     CDS             2879..3478
                     /locus_tag="ACHARQ_002364"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8410755.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            3494..3970
                     /locus_tag="ACHARQ_002365"
     CDS             3494..3970
                     /locus_tag="ACHARQ_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410756.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            3987..4889
                     /locus_tag="ACHARQ_002366"
     CDS             3987..4889
                     /locus_tag="ACHARQ_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410757.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            4962..5153
                     /locus_tag="ACHARQ_002367"
     CDS             4962..5153
                     /locus_tag="ACHARQ_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410758.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            5317..5904
                     /locus_tag="ACHARQ_002368"
     CDS             5317..5904
                     /locus_tag="ACHARQ_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410759.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            5904..6803
                     /locus_tag="ACHARQ_002369"
     CDS             5904..6803
                     /locus_tag="ACHARQ_002369"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFM8410760.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            6803..7717
                     /locus_tag="ACHARQ_002370"
     CDS             6803..7717
                     /locus_tag="ACHARQ_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410761.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            7769..7984
                     /locus_tag="ACHARQ_002371"
     CDS             7769..7984
                     /locus_tag="ACHARQ_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410762.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         3138 a         1032 c         1700 g         2392 t
ORIGIN      
        1 tctattaaaa aattttatta tgaatttgtt gatttggttg tagaatattc tgttcgattg
       61 atcgttccgt ggagtaaaga agaaattaat gaggcattaa aacatgaacg aaaaagtaca
      121 ttcaaagttt tagaaggtga aaaaaagtga aaacagacct tactaggcaa gctgagaagt
      181 gcttatggca tgaaacaaat cgcatgggtg tatttggttg tttcgaagtc actatcggtt
      241 gggatggcag tgaaagagta gattatataa cttattcagt agataacaca gttcgatgtt
      301 atgagatcaa agttacttta gctgatttaa aaagcaaggc gaaacagact tttatcggtg
      361 attataacta tttagttgtt actagtgatt tgtgggagaa aatgcaacaa gacgataaac
      421 ttatgcggaa atattactat caaggcgtat acgtgttcaa tgaaaaatat cctagcataa
      481 taagtgttaa gaaagcaagg aaacaaaaag tatcgctagg tacaagatca actgtattag
      541 aaagcatggt tcgttctatg aatcgtgaag ttaagaaact ctataaagta aaaccatttt
      601 gggagatagc agaggtggaa aaaggatgat tcaaacattt gcaaaaatga gtagaagtgc
      661 ttggattgaa aagctggaaa atgatttgag tgttatgcga ttagctccaa agaaattcaa
      721 acgagagatt gcagaaaaag aagcaatttt gtcggtattg aatgcggtag aactggacga
      781 accgaaaaag tcagttgatg tacctgaaaa tgtagcagag ttactagatt attatcgtaa
      841 ttcgtcagat gtcgatttgt tagctctgat attaacattt cacgattggt attactgtca
      901 aaatgaagat actcagcatg ataaacatga taaagcagtt gactggttaa tagagaatcc
      961 agaaaagttc atgcgtgctt ggctagatgg ctatgatagc aagaaaaagc cactggaaag
     1021 taatgaggaa acgtatttaa cggcagaaca atatgactat gctagaaaaa caggagattg
     1081 ggaatctggt aagactttca atcacagaaa agatcaacca aaaattagtt tttcagatat
     1141 agccgcaatt atcgaaggta tctactatgg acaagccgat gaagagatct tttctataca
     1201 agatttatta gaatttttga acaaaaataa ggatagcttt gtagaactaa ctgattatga
     1261 ggaggataca gcaaatggaa aattatgatc caaatatgcg atggggtaca catacgctta
     1321 aagtttcttt tcaacgttgg gattacaagg gatttgtgac ttttagaaaa gctggaaatt
     1381 gtaaaggatt agatgtctta gcgttggatg aagactatct atatgaccat ccattgacgg
     1441 ataatccaat cggatttgga ttgcttcctg aagatgatga gggcaatgaa tggttcaaaa
     1501 tgattcttac aaacgataaa ggtgatcaat tatttgtaga agatacttgg tcatatttga
     1561 gtgaatatat tgttagcatt aaaatcattg attttgtagc agataaagaa aaagagattg
     1621 gagaaggtaa atctaattac tagctaatga atagcaactt tggaggataa gtcaaaagaa
     1681 taaaaaaaga atactgtgtg tcattgcagg gatgtatctt tgcatgatag tatctgcggt
     1741 taccatttat tggttaggaa atcagcaaaa cagtttaatt gttgatctgg gggagttaaa
     1801 ggttggatta cgaatgacta gatggctttt attagctcaa acagttggtt tagccatatt
     1861 gacttggata tgtgtggaaa aaataacatc taataataat caagagattg ttgaatcaac
     1921 aaacctaaga ataactgaac aagcaattga aaagcaaaaa gacaaagaaa aaaaggaaat
     1981 aatgtcaatt tatttatcta gtggagcatc attttttcat gaaatagatt cgatgaaact
     2041 gagtagtcaa gaaatcattg atagatggaa aaacatgcct ctaggtggtg agttaatcgt
     2101 tgaagatagt gatggttggt cagcttataa gaaggacgag attgtagcta tcacgatttt
     2161 gtttgaatag taatatatta ccactagata atctgaatat gttgatctgc tagtgacgta
     2221 ccaaatgcgt ttaatagaac cttggtcaca ggcagaaatt gataaagcag taaaattgaa
     2281 ccgattatca aaatttaacg tattaggaaa tgccttttaa aaatcgcaat acacaaacag
     2341 aaaaatacca aaaaattagc aatgaacgta ggtaagaaca tgacaataaa taataataac
     2401 cctaacaaat ataatgctct tgtccaatgc tatatcgaaa acaaaattac aatgcaagaa
     2461 gtcgctgatg aattaggcaa atcaaaaagc ctagtatcac acgactttaa gcacaatgtt
     2521 tttccgaacc cgattgagct gaggaaaaaa tttcaaaaga gagtttatca agaaataggg
     2581 aagtggattg atgaagggaa tgcaattgac cctttaattg aaaaatatcc ttttttaaaa
     2641 agcaaaaata aaattcaaag gttaattcgt caaggcgata ttcaagctaa agaacctgtt
     2701 ttgacaacca atgatttggc taaaataaat cttcataaac agattattga agactttgaa
     2761 aaaaatcttt caacaactga aattttaaaa aaatacaata ttccgagagg aaagctaaca
     2821 tcgactcttt ttagagaata tggagaatcg gcagttacta aagcaaagga agtagcgaat
     2881 gagagaaaaa aacaaaaata gtagtcgata cttagtagcg actgtagttt ttgtttgtgt
     2941 attgattttc ctgtttgatt ttttacagat tggaaaaagt ataattggaa atacattgag
     3001 tgcaaaggaa atggttgctt taggtggttt gtatgaagga catttgttcc cgaatatatt
     3061 catttctatc gtttctcata ttaacttgta ccatttaatt tctaatttat tgttcttagt
     3121 agttcttgaa gaagcactga atgatcagta tgagtggtta acattattga tcgttttaat
     3181 catatcagga tttctaggaa gcttagttcc ttttctgcta caaagcaaca tcgtaagtat
     3241 ggggcttagt ggaggtattt ttggatggat tgctttattt gcgattgcat acatccattt
     3301 tgatagcaag gaacatttat tactaagtgt ttttgtatta ttgataatcg gcattttatt
     3361 ggtatctact tttgaatcag gcgaggcaaa tgtcttgtca catgtaatcg gatttgtctg
     3421 tggaatgatc ttgggattta ttctaaagaa cgtttcattg atcaaacaaa agaattaaaa
     3481 gggagtgcta aaaatgccaa aaagaaaaga aaagaaaatt ccagtgtttt tgtctgtctc
     3541 agaaatgtta tcacttcaaa tactgttaag aaaaaagatt gaaacaatag acgaagaagt
     3601 tagaaataaa agggctatgt acgaacaacg tgaagcgcta agagaacgaa tccactattt
     3661 tgatgatata cacactaaaa tctatgaagc acgtacaaat cacgaagacg aaagtaaatt
     3721 aggattagct caacaacgga tacttgaatg gcttaatacg aatactgatc aagaaccgtt
     3781 aagagtgatt agtaatcttt ttgaatttgg aacgaagcat tggtttaatg ctgtagagaa
     3841 ggatgctgac ttacaagaag cgtttgatac gctaagtaag gatagcaaat ccatgcgaga
     3901 tttattgatt acttatctaa atgagcatga gtttgaacaa acgaaaacca aaaagaaaaa
     3961 taaagaatag aaagttgtga tgaatcatgc tattaaccct gttaggttat ttatgtttaa
     4021 ttcttgcttt ttttggggca atcgctataa ttattggtca atttcaaaca aataatccga
     4081 ttatgcgtaa cataggcatt ggaattgtga ttgtaacctt tattgtgttt aatgccacta
     4141 agatttttgg aagtaacgat aaacaatttg gtatatcggg agttaataat agttatgctc
     4201 cagattcttt gggaagggta gtaatagatg gaacatttga aaatgtaacg agttataaaa
     4261 atgtttcgct gacagtgaag gaagatggca agaaagcatc agacgacgtt ctcgctacta
     4321 ttaagccgtc tcaatcacaa tttgaagtgt tttatacagt taaagagaat agccctgtac
     4381 acaagctgac attggttttt aacgttgatg gagaagaata ctataaagat gtaaaaataa
     4441 ataaaacatc aacgaaaaag attatcgaat cagcgaaaaa agaagtagct acagaagaaa
     4501 gtacaactga atcaagttct actgtcgaag cggaactacc agataaaatt gattttgatc
     4561 ctgaaaaagt caaatcgttt gaatctgttt ctggttctat ggctgatcgg tttaacactg
     4621 aagtcttcaa agctatcaaa actgttaaat tggatactga aaagcaaatg attaatgtta
     4681 ccttattcaa ttcagttgaa tctatggatc aggataaaaa agttacggtt gcaagagaac
     4741 taggaaaaag agtaattggt ttgtatcgtg gtactgctaa accagattca gacggtgcag
     4801 atttgccagt tcatgtagtt tatgaagacg gtaaagtttt tggagattct acagcaaacg
     4861 aagcattaac gattaaattg gctaaatagc cagaaagtta taataaacaa atggtgtcac
     4921 accaataata tataggtgtg acaccttgga gatgatagct gatggtagta aataaagaaa
     4981 aagttagaaa gtatgtgact ttaaaaatag cttctgttaa atatttagaa aagcgagcta
     5041 aagaagaaga aagagcaaga ggtaagaaag taacagtttc agagttgtta gaagaattga
     5101 tcgagaatga tcaaacaata agacgtgcat ttactaatag gtctatcaga taacaatata
     5161 attaataaaa aagaaagagt gatttggtct tagactaaat cactcttttt atataaagtt
     5221 tctttacact cattccgaat aatgttaaaa ttctttatat gatatatagt tccttgtcag
     5281 aggaagagat aaccagcaat attggaggga taataagtgg aaaacaataa tgatattaga
     5341 cattttagca ttggggaaag agttacaaca gtaagtgaat tatcaaagga ttatagagat
     5401 aaagagggaa cgattatttc tcttaataag aatcgaagag gcgtttggca atatgttgtt
     5461 gatttaggtg atggcgttac agttgagtgt aaagatagtc aactgatcag aaataatggc
     5521 agtcattcca ttgaaaaagc aattcttgta tacccaaaag tatcaagtaa gatcatgaat
     5581 gactggttgc ttggaaaatt tggtgagtac aataaaaaag aaatcgcaga agtgatcggc
     5641 aaatcttatc caacagtatt agggtatcta aaaaagataa cgagtaaaga agcaaaaatt
     5701 gaggatgttt taacagtaag agagcttcaa tcattaatcg agtatcggaa taaacaaata
     5761 acaaatcctg agtaccttag aaaagaagat ggtctattga aacaattgga acaagtaaat
     5821 aaaatccgga agatgcaaaa ggtagctcct ttaagccttg atacctttaa aaaaatctat
     5881 ttgaaagaga tcaaaaaaga ctaatgtgga ctgtaacttt accgttccaa cctgtatctg
     5941 caccaagacc aagtgcaaga aggaatgctg ctgatgaaac taatgacgta gcagagaaaa
     6001 ggatttcaac ctactatgat aaaaagtatc ttgattattt gaaaaaaata aagagcttta
     6061 tcgaagatca agatttatta gatgatgaat tttactcgat tgtaaatgat cctatgggcg
     6121 caattatgga agtggacttt tactaccaga taccaaaaag ctataaaaaa gtctataaca
     6181 ttatgaagac aacagcgcca gatttagata atttagtaaa aagtgctatg gatggtattt
     6241 ttaacatctc agctatacga gatagccata tcacaggctt aattgctttt aaatataatg
     6301 tggcgaacga aggaaaaaca gttgtgagga tcaaacgata ttcagaattt gagtgggata
     6361 cttctgaagg attgaacgga gatatatggg aagctacatt tgattttaaa ccagtagcaa
     6421 cgccaagacc aaagtctaaa tttagaggaa atgggattat cacttactat ccaaaagagt
     6481 acaacgacta tttggaaaat atcaagaata cgcttaaaga gaaagatttg gtaaatgatc
     6541 aactgaaaaa ggtaatgaat gctcctatgg gggttatagc tcagatagat tatttttacc
     6601 aagtgagaaa agataagaaa aagcttgatt cactgatgcg tacttcacag ccggatattg
     6661 ataatttagt caaaggaaca ttggacggaa tttttaacaa ggaaataggc atcaaagata
     6721 gcagggttgt agggttaatt gcttttaaat acaatacttt tgaaaagtcg aaaacaaacg
     6781 tgagactaca ggagatgggc tgatgaataa taatgaaaaa attcttcatg tacttgatag
     6841 ctttgaaata attcaagaag aactaaaaaa atatcgggat gttttggaac aaagatatga
     6901 ttttgtcaat cagcaaaaga gtaatcacat ggatttcatt ctcaatatga atgacttgaa
     6961 gaagaaatta gttgaacgaa aagaacaaga aaagttgatc aaagcttact ttgaactcgg
     7021 tgaaaaagaa gttaaaaatg caatggaact gaatgaagag cgcagggttt tagatcagct
     7081 gttagaacaa ttattggtga tgtttcaaaa aggacgaata gatgaggatt taattgaaga
     7141 aggattgaga aaatatcctt caaactcagg tatcggaata gtgctgaaag cgattgatga
     7201 agaagaaatt gaagctttca taccgccaga ggattttgaa tcggcaatgg aatatataaa
     7261 atattatagc caagggatca ctgcttttag agagtttgat cctgaagatg ttattcatga
     7321 cttgaacaac ctaaaagaat ggtgtgaaag ttatggagtc gatgattccg gattggacta
     7381 cttgatcagt attatggaaa tcgaagaaga gatgccggat aagcctgatc caacagatat
     7441 tttagaattg atccatgaag ctagaaatcc aattgcctat atttctagag gatatactgt
     7501 attggaatac tataagcctt atatatcagc gatgaaccat ttaagacgtg tattaaggga
     7561 aaaacgtgaa taccgctcag tgttgaatgc aacgaataga ttagaaaaag ctgtttcaga
     7621 gctagatgct tattatcgtg aacattacct gcaagcaggt ggtatgccaa gaaatacgaa
     7681 agcaaatatc agtaggtata taaaaaaagc tgagtaggcg gagttagccc ataaaatggc
     7741 aaaaggtaac ctgaaagggg cattttgaat gaaaagggat gtttttgaat tgtacgtatt
     7801 agaacgtcta gaagacggca tgatggcaca agagataatt gatgaaattg atgatatgat
     7861 cagcagattt ggtcaattgg agttgaatga tggaaacaaa aattcggtag tgctaaaaca
     7921 attttggttg aacaattttt cgtataagga ttttatcaaa gaaaaggcaa atacattgta
     7981 ctgactaaat tcacgaggag cagtgatgag agcttattgc taagagcagt taggctcttt
     8041 tttgtttgtg taaaattatt tgttttataa tacacattta tacacaaaaa aatattgaca
     8101 gatagtacac aaatgtggta ggatatattt gtaagcaagt tagattaaca agttaaagga
     8161 agagcagtta tgaaaaaaga agaactaata gatatatttc aaattgttga acgagcaaat
     8221 aatatgggaa ttatgttttt tgatcgtatt tctttaaaga tg
//
LOCUS       DAYYAG010000120         8149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000120 DAYYAG010000000
VERSION     DAYYAG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.123"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHARQ_002372"
     CDS             476..1339
                     /locus_tag="ACHARQ_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8410763.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHARQ_002373"
     CDS             complement(1468..3090)
                     /locus_tag="ACHARQ_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8410764.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHARQ_002374"
     CDS             3339..3860
                     /locus_tag="ACHARQ_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8410765.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHARQ_002375"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHARQ_002375"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8410766.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHARQ_002376"
     CDS             5941..6759
                     /locus_tag="ACHARQ_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8410767.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHARQ_002377"
     CDS             complement(6862..7059)
                     /locus_tag="ACHARQ_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410768.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHARQ_002378"
     CDS             7228..7806
                     /locus_tag="ACHARQ_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8410769.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHARQ_002379"
     CDS             7901..8038
                     /locus_tag="ACHARQ_002379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410770.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2435 a         1474 c         1608 g         2632 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagt
//
LOCUS       DAYYAG010000121         8113 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000121 DAYYAG010000000
VERSION     DAYYAG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.124"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(470..832)
                     /locus_tag="ACHARQ_002380"
     CDS             complement(470..832)
                     /locus_tag="ACHARQ_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8410771.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            1055..1807
                     /locus_tag="ACHARQ_002381"
     CDS             1055..1807
                     /locus_tag="ACHARQ_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8410772.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1947..2870)
                     /locus_tag="ACHARQ_002382"
     CDS             complement(1947..2870)
                     /locus_tag="ACHARQ_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8410773.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2885..3157)
                     /locus_tag="ACHARQ_002383"
     CDS             complement(2885..3157)
                     /locus_tag="ACHARQ_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410774.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(3501..4688)
                     /locus_tag="ACHARQ_002384"
     CDS             complement(3501..4688)
                     /locus_tag="ACHARQ_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8410775.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(4795..5007)
                     /locus_tag="ACHARQ_002385"
     CDS             complement(4795..5007)
                     /locus_tag="ACHARQ_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HFM8410776.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(5098..5550)
                     /locus_tag="ACHARQ_002386"
     CDS             complement(5098..5550)
                     /locus_tag="ACHARQ_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8410777.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(5563..5973)
                     /locus_tag="ACHARQ_002387"
     CDS             complement(5563..5973)
                     /locus_tag="ACHARQ_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8410778.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            6248..6460
                     /locus_tag="ACHARQ_002388"
     CDS             6248..6460
                     /locus_tag="ACHARQ_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8410779.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            6463..6675
                     /locus_tag="ACHARQ_002389"
     CDS             6463..6675
                     /locus_tag="ACHARQ_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410780.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            6815..6970
                     /locus_tag="ACHARQ_002390"
     CDS             6815..6970
                     /locus_tag="ACHARQ_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410781.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(6967..7191)
                     /locus_tag="ACHARQ_002391"
     CDS             complement(6967..7191)
                     /locus_tag="ACHARQ_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8410782.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            7269..7457
                     /locus_tag="ACHARQ_002392"
     CDS             7269..7457
                     /locus_tag="ACHARQ_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410783.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            7596..7724
                     /locus_tag="ACHARQ_002393"
     CDS             7596..7724
                     /locus_tag="ACHARQ_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410784.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            7783..>8113
                     /locus_tag="ACHARQ_002394"
     CDS             7783..>8113
                     /locus_tag="ACHARQ_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410785.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEF"
BASE COUNT         2655 a         1451 c         1368 g         2639 t
ORIGIN      
        1 cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag aaattagcgt
       61 tttctatttt tcttacttga taaaataagt agattttcct actacaatcg tagccgttaa
      121 gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa tggcaaaagt
      181 ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa acggatataa
      241 cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac cttgcgttga
      301 aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag aaagcagctg
      361 tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt tttttattgt
      421 atacattaga tatggagcag gttaccataa agatgattat tagtcgttat taaatagaat
      481 ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg aattgtattc
      541 cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct cgtgtcattt
      601 ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc ttcgtattga
      661 tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt cgatctaacg
      721 aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact actttaggat
      781 aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc atctcattcc
      841 tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct ttcctagcat
      901 taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt atcacttaat
      961 agtataacat aatttcgtga gaaaaaaata caattttttc ttgaccatct cgttacgtaa
     1021 aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa agaaaatgtc
     1081 agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg gactgctaaa
     1141 accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag tcgatcgtct
     1201 gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc aagaggtctt
     1261 agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa tgttgctgga
     1321 aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg atatgtacga
     1381 aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac aaaagttgaa
     1441 agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa aaaaggttac
     1501 agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa tgcaaactgt
     1561 tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag atgaaagctt
     1621 agcagaccgt atcttccaag ctcataagaa gtggttctct tacagctggc ctgaatatca
     1681 gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt tcactgcata
     1741 ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta tccaaaaaaa
     1801 ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta ataacaaaac
     1861 agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat tctttcacaa
     1921 gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg tattttcact
     1981 ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc ttccagatgg
     2041 tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg tccaatcacc
     2101 actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat atccgaattt
     2161 gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt tcctttctct
     2221 gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag ttgatgacat
     2281 agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa cgtgatgccc
     2341 aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga ttgttctcga
     2401 tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac tagaggtcca
     2461 tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat gatcaatcca
     2521 tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt atgatagccc
     2581 gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat cccactgatc
     2641 aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct tttcgttttt
     2701 ccataactta actgtctggc atttgcatta gggatataat cgacttcttt catgaccgca
     2761 agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac acgtgaaata
     2821 gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac tgctgcttcg
     2881 ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg cattcaaaaa
     2941 actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa tatatatctt
     3001 ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta ttcaaccctt
     3061 gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa aaataattgt
     3121 tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct tttgatactt
     3181 tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa agaaaagttt
     3241 tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt ataatgatac
     3301 tgtaaatata tctttattat tagtgaatgg agtcaagtcc acaatcttgt gtaaaagttc
     3361 aacctcatca aaagcacaat gttttgcgtt gtgaacctaa ctgccatgcc tttctttctg
     3421 aggagagagg tcaggagcag tagcgcagcg actcttgatg tctctcctca gaaagaagag
     3481 actcgcacag gttcatggtg ctaaatgtta ttcgaattaa attctaaata ttttcgtgga
     3541 gaagccagcc actcagtgtc tttttccatc aaaagtgttc caattatccg agtgatggaa
     3601 ttgatatttg gaaaaatccg aatcactcgt tctctccggc gaatctcttc gttcaaccgt
     3661 tccaacacat tggttgtacg aatccttcgg cggatatttt caggaaaaga caaaatttga
     3721 atagcatctt caaagccgtt ttctaaaatg tcgcaagcag cacttaattt tgaatcacaa
     3781 ccatattttt ccaagaagtg ctcttttctt tcacgtgtca attcaagttc agaggcttta
     3841 aagatactct tcaactcgtc ttttacatca gaagaaactt tctttggcag cttatcaaaa
     3901 atatttctca aaaagtgggc ttggcatctt tgccagctcg cacctaaaaa gttcttgcga
     3961 acagccttaa ctaacccttt gtgtgcatca gaaatgacca gttttacacc gaacaaacct
     4021 cgttgaacga acgattgaaa aacggtatcc caattatctt ccgattcgcc gtcttgaaca
     4081 tcaaaaccaa gaatcgtacg tcgtccatca gaatcaatgc cgataccaat atatacacct
     4141 tttgagacaa tacgatgatt ttcccttact ttcatataca gggcatcaca catgaaaaat
     4201 ggatattccg agccttctag gctccggttt ctccactcaa aaacaaatgg atccaactgt
     4261 ttcatcagat ttgaaacgaa tgatttagaa acagtcgcac catccgttag cagttcaact
     4321 gttttagtta ctttgcgagt ggagacacca gaaatgacca tttcaatcat ggcagcgatc
     4381 aaagcttttt cgcttcgctg atatctttca aagatagtag gactaaactt accgtctctg
     4441 gttcgtggaa catgaagttc taacgtacca attcgagttg taaacgaacg atcgtagtaa
     4501 ccatttcttt gtgattgccg ttcttctgaa cgttcatagg catttgcctg aatatattga
     4561 tttcgttgtt cttccatcag ctgattaaac atgatagtca taagtgtttt tgcggtatca
     4621 ttcgcaccgc tttcgtttat aattgcttga atttcttcgc tttcaaaagt aaaatgtact
     4681 tgagtcattg atatcagtcc tattcgagta tgttttctgc aaaaaacatt gtactcctaa
     4741 ctgagatcaa tggctttttt gatgcttcac ttttacacaa ttatatggac gtaatcaatt
     4801 aatatcattt tttaacatag taatttgttt atttaaatct gatagtgttt cttccttact
     4861 taatataaat ctttctctat catgtaattt atttagtttt tcatcatttt tttgttctaa
     4921 ttcataagct tcttttttta ttgtgttagc tttcgacagt ttagtgattc ctataattaa
     4981 taaaataagt ccaatcaata ataacattca aaactcctca tttctttgat ataattattg
     5041 tagagtttac tcagaaatga ggaaagagtc catgtgaaag catgggcttt tttattttta
     5101 atgttgtaat actaaatgtt caagattatg aggaagccct aaatattcaa taatttggaa
     5161 ctgactcata tcagataaat cttgttcatc tattgttcta gttaacattt caattgcaaa
     5221 catattagct tcagcttcta taccataaat catacaacta aggttgtttg ctctaaaagc
     5281 tgctgtgcaa atacctttat ggagtctaac gtgggctatt tcatgccata atacaaatct
     5341 tttcatagaa ggtgataggt tttgattaat taatatcaca taacatcttt tattgtgtat
     5401 cgtttgtcct aatgttttat tccctaaatc agtttcaatt aaatctatat cagaatattt
     5461 gattatttta tatggattaa tcgcatcaga tccaaactct acaaataatt tttctatttc
     5521 ttctaatatc cagtatttgt ttaaatgcat accaaagccc acctattgac gatatttttt
     5581 aggagtgaat tttttctttg ctttaacttt ggttaaggca agtgcaagtt gaatggcatc
     5641 gtataatttt tccttatctt catcgtctaa aggttcacca taataatttg tttcagcatc
     5701 tgaattcaag ccttcaagta atttttcagc ttgtaaagca atatcttgtt tatctttttc
     5761 tgttaaatca taataatggc gtttatcagt acgtcctaaa agataatcag ttgatacatt
     5821 aaagtaatct gcaacttttt gtattttatc tccagatgga gtacttttat tccatctaga
     5881 catgcttcca tttccgaaat ttaactttct ttcaagttct gccaacgtca ttgaacgttc
     5941 ttttaataag atttttatac ggtctaatag cataggatat gcattccttt caaaataccg
     6001 acaaaaaaag tatttttctg caaattatct atttacaaat agagaatttg cagttatact
     6061 agttttgtaa gcaagatatt tacggcatac aaagactaaa agcaataaac taatagtctc
     6121 cccagacatc agtaagctcg ctttttgtgc gctgtatttt gtatgcttac ataatagcat
     6181 atttgcagtt tttgtcaaac ttattagaca aaaatattag aaaatattcg ataagggggt
     6241 atgaagcatg actttaaaac agcaagtatt aattgcttta gtaaagaaag gttggagtca
     6301 aagagagtta gctcgacgaa tgggtatatc aattacttat ttacgagata tcttcatcga
     6361 aaaaagaaaa cctaaggaac gtttaaaaca aatcgaagaa cttttggata tcaagttaga
     6421 agtagattct aatgatcagc catcagaaca ggaggcgtaa aaatggtaca acgtttgagc
     6481 aaagcggagt ttaaagagaa atataagtat tctgaaagta cttatcaaag aagaatgaag
     6541 gaatttaaaa aatcacgttt tagtgagggg tataacgcag ttacttccaa agaaataaca
     6601 attgatgtcg atttatatga acagtttaga acatggcgtt ctaaaaatcg atttagagtt
     6661 agagctgttt gttaatagct aaaaaatcta aattcaccac ttctgaaaag aggagttgct
     6721 gctctccttt caagtttgcc aaagtccacg ttttttcatt ttggcaactc ctcttcttgg
     6781 aggtggtgac ggcgagagac gaaggaggga tgacatgaag gaagaatcaa aaatagggat
     6841 tttgaatgag aacaattaca caagattaat tattgaaaca gaagaaggga aaaaagtagc
     6901 agaagttaca ttgaccgatg caactcctgc tgacggttat cgtattcgat taactcctaa
     6961 atatgattaa ccttttggtg ggtgtggatc gtttccgtga ctatcacggc tttgaatttt
     7021 tccatctctg ccatgaataa atagctcaga tccttgattt ttagaaatct ctctagcgat
     7081 ttttactgct tctgattttg tattagtatg tgcagtcgct ttagaatttc cagcaccttt
     7141 gacattccaa ccaccattgg aattaggtac aacatgttga ttttttccca tagttttcac
     7201 ctccatttct atagaaatat tttatcaaat agaaatggag aaattcaata aacgaaagga
     7261 gaagtgatat ggcatatacc ttgcaacaag aacatcaaat tctcggtttg attaaacaac
     7321 gcaggaaaca attacaagat gaccgtgcag cgcttagaaa agccgatgag ctatcagata
     7381 ggcaagctga actaattgct tctgaacttg aggatttgag aatgctagaa ataaaaaata
     7441 gggagattag attatgaaga agacagacac actttttata ggattcattt tggggttatt
     7501 agtgattgta gcgcaccaaa gtattatcgg gggaagcttg ttcgcagcat tgatggtttt
     7561 aatcaatctg cttgattcaa aagaaaggag caactatggc acgagaagaa gcgctaaaaa
     7621 tcggtaaagt gattgctgat aatcggtggg gaaatagccg ccctattatt ttaagcaagc
     7681 aacatatcga aaagcaaaaa gcatggcaac aaataaaaaa gtgactctgc cagccagcaa
     7741 agagtcacat acaaatttat aaaactagga gaattatatc acatgaataa gaaaattgaa
     7801 aatcttatcg aagaattgaa acgtgaatgc caaaagcaag gtgtttctat tatttgtaca
     7861 gcccaaaaag aaggggaact taaaagtctc gtttatggtg agacaacaga aattttactt
     7921 tgtcttgcta tgcaagaaga acatttagat gaaaatctcc cattgtcagc acatattatg
     7981 cgtagaattg ctgtggatgc ttacgaacaa gcgaaaaatg aagaagaaaa tcaaccttca
     8041 aatcatactt ttgttattaa taacaaggaa gatttagctg atgtgatgac tcgtattctc
     8101 aagggggaat ttc
//
LOCUS       DAYYAG010000122         7983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000122 DAYYAG010000000
VERSION     DAYYAG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.125"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..839
                     /locus_tag="ACHARQ_002395"
     CDS             63..839
                     /locus_tag="ACHARQ_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8410786.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            854..1057
                     /locus_tag="ACHARQ_002396"
     CDS             854..1057
                     /locus_tag="ACHARQ_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410787.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1073..1411
                     /locus_tag="ACHARQ_002397"
     CDS             1073..1411
                     /locus_tag="ACHARQ_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8410788.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1398..1577
                     /locus_tag="ACHARQ_002398"
     CDS             1398..1577
                     /locus_tag="ACHARQ_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410789.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1620..2090)
                     /locus_tag="ACHARQ_002399"
     CDS             complement(1620..2090)
                     /locus_tag="ACHARQ_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410790.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2177..2875
                     /locus_tag="ACHARQ_002400"
     CDS             2177..2875
                     /locus_tag="ACHARQ_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8410791.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3053..3394
                     /locus_tag="ACHARQ_002401"
     CDS             3053..3394
                     /locus_tag="ACHARQ_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410792.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            3387..4058
                     /locus_tag="ACHARQ_002402"
     CDS             3387..4058
                     /locus_tag="ACHARQ_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8410793.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4064..4756
                     /locus_tag="ACHARQ_002403"
     CDS             4064..4756
                     /locus_tag="ACHARQ_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8410794.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            4753..5502
                     /locus_tag="ACHARQ_002404"
     CDS             4753..5502
                     /locus_tag="ACHARQ_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410795.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            5514..5783
                     /locus_tag="ACHARQ_002405"
     CDS             5514..5783
                     /locus_tag="ACHARQ_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410796.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            5789..5953
                     /locus_tag="ACHARQ_002406"
     CDS             5789..5953
                     /locus_tag="ACHARQ_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410797.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            5946..6245
                     /locus_tag="ACHARQ_002407"
     CDS             5946..6245
                     /locus_tag="ACHARQ_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410798.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            6245..6547
                     /locus_tag="ACHARQ_002408"
     CDS             6245..6547
                     /locus_tag="ACHARQ_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8410799.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            6587..6952
                     /locus_tag="ACHARQ_002409"
     CDS             6587..6952
                     /locus_tag="ACHARQ_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410800.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            7442..7582
                     /locus_tag="ACHARQ_002410"
     CDS             7442..7582
                     /locus_tag="ACHARQ_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410801.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            7579..7761
                     /locus_tag="ACHARQ_002411"
     CDS             7579..7761
                     /locus_tag="ACHARQ_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410802.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            7774..7968
                     /locus_tag="ACHARQ_002412"
     CDS             7774..7968
                     /locus_tag="ACHARQ_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410803.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         2968 a         1227 c         1661 g         2127 t
ORIGIN      
        1 aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa ggaatgagaa
       61 atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca attttagtaa
      121 atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat tcaaacacta
      181 aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga atcacgacct
      241 caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt ttaatcttaa
      301 aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa gtgttgccag
      361 caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct ttgcttaatc
      421 cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga agattaatcg
      481 cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc atattatctt
      541 ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct ccacaacaga
      601 tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa tggttattgt
      661 gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc ccgaaagccg
      721 atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt ctatttatct
      781 acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa attggttaga
      841 aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag aaaaaatcaa
      901 agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa gatttggaat
      961 tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag aagcaaacag
     1021 aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc taatgcaata
     1081 tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag tggattatga
     1141 ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag tgctagaacg
     1201 gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa gatatagaaa
     1261 tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg aaggaagaga
     1321 cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt tttcggaaat
     1381 cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc gcaccgctaa
     1441 tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca
     1501 tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa
     1561 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
     1621 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
     1681 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
     1741 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
     1801 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
     1861 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
     1921 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
     1981 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
     2041 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
     2101 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
     2161 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
     2221 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
     2281 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
     2341 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
     2401 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
     2461 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
     2521 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
     2581 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
     2641 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
     2701 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
     2761 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
     2821 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
     2881 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
     2941 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
     3001 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
     3061 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
     3121 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
     3181 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
     3241 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
     3301 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
     3361 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
     3421 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
     3481 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
     3541 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
     3601 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
     3661 gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac
     3721 caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac
     3781 acggactggg attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata
     3841 caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag
     3901 atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag
     3961 ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag
     4021 caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat
     4081 cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa
     4141 tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt
     4201 cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa acttcatcta
     4261 tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct ggaaggaatt
     4321 cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag acgaatcgaa
     4381 tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca tctttgaaca
     4441 tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat attacttcta
     4501 caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg acatcgatca
     4561 ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag agtacacatt
     4621 tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga ttaatttcaa
     4681 aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa agttaaatat
     4741 tggaggggaa aaatgacaga acatcgaagt tattatgcga ttataccagc caacgtaagg
     4801 tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac ggccttgtgt
     4861 aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata tggtgtgaat
     4921 aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt gaatcgggaa
     4981 atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat taatcaatac
     5041 cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa taatacatct
     5101 attaataata catttaataa tacaaaagaa tatataagag agttaccgcc ttcgaaaaaa
     5161 tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct tttgtcagat
     5221 gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg aatcgaacga
     5281 ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc aactattcga
     5341 agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata caagcgcaca
     5401 ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct caagaaaaaa
     5461 gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga aaaatgaaga
     5521 tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag gaattagacg
     5581 tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt aagaatgatc
     5641 gttcgagttg gagtaaactc agatactatt tcgcactcta tatcgatgga acgcaattaa
     5701 gaaatttagc atataccaaa ttactattca ttgattgtgt taaaggcttg caaaaacatc
     5761 ttaatgaact tgagcaggtg taaacaagat ggacctaaag acatttacag cacagatcga
     5821 attaatgcat caagaagctt taagacaaag tgtgtcgtac gaagacaagt ggctcaacac
     5881 atttcatggc ggacgtgaga gcgcacttga tcaagtactc aaattactga aaggagaatg
     5941 tcgggatgga taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
     6001 ggcaggaaga taaaaaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg
     6061 aaaagcccaa acggagaacg ccgagaaaga ttgcaatctg gcatggtgat cgaattctag
     6121 taacgggtac cgctaaacag ttatctgaaa ttactggtct gagcaaaaat attatttggg
     6181 atagagctag gagcttatgg attgattcaa aaggaagaca gtttaggtat gtggaggaga
     6241 aataatggac gaactaatcg cgaaagtaga gcagtgggca aaagataagg gattggatca
     6301 agcggatttc agcaagcaaa tgttaaaaac ggtcgaagaa atcggggaag ttgccgcttc
     6361 tctagctaga aaagatgaac atggtttaag agacggaatt ggcgatgtag tagtaacctt
     6421 gatcatcttg gcaatgcaaa atgatatgga tttatacgag tgcttggatt ttgcttatga
     6481 cgaaatcaaa ggtcggactg gaaagatggt caatggtgtt ttcgttaagt ccagtgacct
     6541 gaaataattc cattaatgga agaaataact gaataggagg atagacatga ttgatgcaaa
     6601 acaagtgcaa aaacagaaag atggaatgtt gatgtttgaa gcatatgttc taccatttct
     6661 taatcaattt gaagttttgg aatgctctgc cagcggagaa gaattggagt atgttgtaat
     6721 tcgtgaaacc aaagaaaacg ttcagaagct aaacgaattt ctttgcacta ttaactgttg
     6781 ggatatgata gctccaggat ttttatgtcc agctatggga gaatttttgg aatattgtcg
     6841 tcttgaagat gcaggcactt tggatttagc ttatctggta tacaactacc taaacatcaa
     6901 cacagatcat ttgtggtttg gcactgccga aagaaaatgg gtagtccgct aaccaccaat
     6961 atcaatgaaa aaaggcggtt tcctgaaagt ggaattgccg ccattgctgt aaggttcttt
     7021 acgcgactca aggtcacaga aatataaatg caaatctctt ttgaagtgat ccaaagctct
     7081 cggaaaatgg aagttttttg tttcaaagaa taaatgagat ttgcgtcaag aacctgagag
     7141 ttatttccaa aaaagcttcc aaagaaccga atgagctaag gacatcatgt gtaaacacat
     7201 aatatagctc tcctttcacg gctaatcata acatgaaaaa tattcgatgt cagtaggaaa
     7261 aaaggtgaat tatgaaaaga atattaaatt atcccggcag taacagattc tgcaattaaa
     7321 ctccgaagct gatgtatcag tatcaactgt agaatattat gagcgtggag aaagtcaagc
     7381 agtcacaaat gtaaaaatcg ataacgtcaa cggtcttatc tgtttagatg aaggcgatag
     7441 tatgaatgaa attgctgcag accatgaatt atcagaaact gctttaattg atcaaatcga
     7501 attagctaga caatactttg ttccatatgc agatacctgg tccaagcata agaatgacat
     7561 catttttcca gaggaacaat gagagcaact gatccagtaa ttatccttga ggaagccaaa
     7621 tttatttgga ctcacgaaga gatagagcaa gcacgcttgc tcttttctca aggagttaag
     7681 ccgagcaaag tagctgaaat aatgggtcaa aagattcttg atgtcggatt gcttttgctc
     7741 catctagcag aaaaaatttg atttgaggtg aaaatgatga ttcaacttgc aggcatccaa
     7801 acagggaaaa tttatttttc tggagaaagc aaaagcgaag ccaaccaatg gctattgaaa
     7861 acttacacga acaataagaa gctaaggaaa aaatatcctg atttgttgct aaaggatgat
     7921 cagattatgc cggaaccgat gattttgacc cgtaaggagg gaagttgaaa ctaaaaaacc
     7981 agc
//
LOCUS       DAYYAG010000123         7832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000123 DAYYAG010000000
VERSION     DAYYAG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.126"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..1427
                     /locus_tag="ACHARQ_002413"
     CDS             159..1427
                     /locus_tag="ACHARQ_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8410804.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            1420..2706
                     /locus_tag="ACHARQ_002414"
     CDS             1420..2706
                     /locus_tag="ACHARQ_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8410805.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            3445..4368
                     /locus_tag="ACHARQ_002415"
     CDS             3445..4368
                     /locus_tag="ACHARQ_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8410806.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            4577..5155
                     /gene="pgsA"
                     /locus_tag="ACHARQ_002416"
     CDS             4577..5155
                     /gene="pgsA"
                     /locus_tag="ACHARQ_002416"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8410807.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            5395..6636
                     /locus_tag="ACHARQ_002417"
     CDS             5395..6636
                     /locus_tag="ACHARQ_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8410808.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            6745..7794
                     /gene="recA"
                     /locus_tag="ACHARQ_002418"
     CDS             6745..7794
                     /gene="recA"
                     /locus_tag="ACHARQ_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8410809.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
BASE COUNT         2602 a         1389 c         1716 g         2125 t
ORIGIN      
        1 aaaaaacatc tatgctatac tataaagtgt aacatcattt agaaaaaacg gcgaattgcc
       61 tgaacttata aatttcaagc agaggaataa gtaaaaaagg caggttgacc cattaaaaga
      121 gtaagcgggc aatcaactgt gaaaaggagc atataaatat ggtgtatcaa ttggcagaag
      181 gcgtgaatct tcatgtctta ccaacaaaac aatacaaaac gatccggatt tttatccgtt
      241 ttactgcgcg tctgcaacaa gaagtcatca cgaaacgttc acttctcagc agcatgttgg
      301 aaacaaatag tttgaattat tcggatcaga caaagttaag tgcgcaactt gcagagcttt
      361 atggcgcaag ctttggatta tcagttagaa aaaaaggaaa tttacattgg ctaaacgcag
      421 ggatcagttt tgtgaatggt gaatatgtca atgatccaaa ccttttttct caagcggtag
      481 attttttaaa agaggtttta ttttatccaa atattaaaaa tcatcagttt gatcagttga
      541 cgtttgactt ggaaaagaac aatctgcgtc tatatttgga aagtttgaag gaagataaac
      601 aaacttttgc ttcctatgcc ttgcaagaac tttattttga aaacagcccg gaacaaaaga
      661 tcccagggtt gggcgtagtc gaagatttgg acaagatcac tgctagttcg ctagcgtcct
      721 actatcaaga aatgatggca aatgatcaaa tcgacatttt tgttgtcggc gatgttgatc
      781 cggacaaagc agctgaagca gttgggcaat taccatttga gtcgcgggaa acagtttatc
      841 cagatctgtt ttatacccaa cctcaagtga atatcgtgaa ggaacgacaa gtacgtgagc
      901 caatcgttca agcaaagctg aacctagctt atcagacaaa tgtgtattac gatgaaccag
      961 aacgttttgc gctaatggtc tttaacggtt tatttggcgg ttttcctcac tctaaacttt
     1021 tcatgaatgt cagagagaaa gaaagtctag cttattatgc ctctagtagc gtggatacgt
     1081 tccgaggatt catgagtgtc caaaccggta tcgatgaaaa gaaccgaaat caagtacttc
     1141 atctgattca cgagcaatta gaaagtttgc gaaatggtga aataacagag ttggaactgt
     1201 ctcagacaaa agcaatgctg agaaaccaat atctgctttc tctagactct ccgcaagcgg
     1261 caatcgaagc ttcttttttg gacagctggc tgccggaaac aaaattatct gatgaagaat
     1321 ggttgaaacg tatggagagc gtaacgatca aagagataca acaagtagct gagcaaatcg
     1381 aattgcaggc aatcttcttt ttagcaggag gaaatgcaaa tgaataaaac agaatatgag
     1441 caaatcaatg aaacacttta tcatgaagta ctgccaaacg gtttgaccgt gtatttgttg
     1501 cctaaaaatg actatcacaa gacatatggt ttgttcagta cgaattatgg ttcgatcgat
     1561 aacgaattta ttccctatgg tgaaaaagaa aaggtgaaag ttccagatgg aattgcccat
     1621 tttttagaac ataagttgtt tgaaaaagaa gatggtgatg tctttcaatt atttgggaaa
     1681 caaggagctt ccgccaatgc tttcacaagt tttacgaaaa ccagctatct gttttctacg
     1741 acggatcagg tagaaaagaa tctgacgact ctgatcgatt ttgtacaagc accttacttc
     1801 acagaagaaa ctgtaaacaa ggaaaaagga atcatcggtc aggaaatcca aatgtatgaa
     1861 gacgacccta actggcggat gttttttggc atactaaata acttgtatcc tacacaccca
     1921 ttgcatatcg atattgcggg cacggttgaa agtatcgaca aaatcacagc tcaagacttg
     1981 tatacgtgct atcggacgtt ttatcagcca agcaatatgg ttttatttgt agttggtaag
     2041 atggagcctg agaaattgat gaaacttatt cgggaaaacc aagaagcaaa aaatttccct
     2101 ccaaagcaag aaattgtacg ttatttccct gaaaacacaa aagaaatcat caaacaaagt
     2161 gcattggaag cagccattac acgagataaa tttgtacttg gtattaaagg attagacaca
     2221 ttgccgcaag aaggaacaga actgttacgc tataaaacag caatcaatct gttgttccaa
     2281 atgattttag gcaatacttc tcgaaactat ctagcgatgt acaatcaagg aattatcgat
     2341 gacagctttg gttttgaatt cagcttggat cgtgaatttc actttgcgga cttcagcgga
     2401 gatacggacg aaccagagaa agcagcagaa aaagtcaaag aaatcattct tggattcgct
     2461 gatgatccgg aagtgtcaga aacaaatctc gatttgctga agaagaaaat gcttggacaa
     2521 tatttccaat cattaaattc gatcgaatat attgccaatc aatttacgca aagtttattt
     2581 ggcgatcgaa ctttatttga tcttcctgaa atcatcgatt cgatccaaat gaaagacgta
     2641 ctggcagctg gggaagcatt tataagtgaa gaggccttca gcgagtttta catgagacca
     2701 aaataaacca gtattttatg aggaaaagtc ttaaatttcg ttttagactt gtagtaggaa
     2761 cctcctccaa aatcagcttt tatggtattc ttagttgtgt tggaaaaatt agtgagaaaa
     2821 taaaacaaat catgctctct tttaatcaac ttatattaaa ataaaatcaa acgaagtaat
     2881 caaagaggtt gtgacagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg
     2941 cttctcaaat ttttgtgata tttcggctta ttgccgaaga agctacttct ggaacaccgt
     3001 gtaccaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
     3061 cagcttctta atgtttttaa cttggttgga cttattaccg cagatgcaag cttttgaaca
     3121 ccgtttaagg aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacatacgg
     3181 aaaaatatct ccttatattt tgccggtttt tgagctaata ccgaggaaca gctgattgaa
     3241 caccgtttaa gaaagaggtc gtgagcctgc cgtttaactc cgagtcacaa ggaacaagtt
     3301 caaaaaacag cttcttatat ttattggata ttgtgacttg tgaccgagga gttggcagga
     3361 gaacaatgtt tattcggaaa aaaggagctg tgacatgact tttgtcacag cacccaaaca
     3421 taacgtcttt cgttgcaaca gcagatgctt cggagaatca aacagactgg agaagatgaa
     3481 cgagtggctt ttgaaaccat cggagaaaaa ttacggcaag cacgcctgaa taaaaaaata
     3541 tctttagatg aattacagca gattacaaaa attcaaaaac gttatttgga agcaatcgac
     3601 aatgatgatt ttgaccaatt gcctggtaaa ttttacgtgc gggcatttat tcggcaatac
     3661 gctgaagctg taggagaaga tggagaccat ttagtcgatg tttttgatgg gaaaagcaaa
     3721 ttgacaggat cggtccctga acgtccgcag cctgaaacag taagtacttc tagaaaagca
     3781 atgcatcagg aagaatcgca tccaagtcgc ttttggacaa gtcttcctgt cattttatta
     3841 ggattggttg ctttggcgat tgtcgttgtc gtgggctata tgacttggca agatcgtcaa
     3901 gctgatccaa taatcggaga cacagcttct tcgcttaaag tagaaggatc agtcgaggag
     3961 tcctcttcct cagagcaaag cacgcagacc aaagagtctt ctacagtaga atcaacaaca
     4021 aaatcaagtg aagaaaagaa aatggcaata gctacaggag aagattctgg cagtgccatc
     4081 gcagtaaata tcacagacgc taccaagcct gtaacccttg aatttactgc tgtcaatcgt
     4141 gtatggttag gtgtgctagt agacaatgcc tatgtttatc aagggacact ggcagcaaat
     4201 gaaacacaga gtaccgtgtt accggaaaca gcaacaaatg cgaccatcac gattggtgca
     4261 gcaagcaatg caacgatcaa agcgaatgga gaatccgtac cagttaatcc aggggaaaac
     4321 aatcagagtc caaaaaatgt gaaccttact cttcaatacg cagaatgatc gtataaatta
     4381 gtttacgcat ctgtttactt gtaggtataa aaaagaagga tcgtgacaaa tctgtgtcgc
     4441 ggtccttctt cgttaattca aaaagatcaa tttcaataaa aatactcctt tgaataaatt
     4501 catttggaaa agtaaataat atcggtggaa acttcgctga tagtcacgta gaatagggaa
     4561 gaaaggagtc gatagtgtga atttaccaaa taaattaact gtacttagaa tttgtatgat
     4621 cccgattttc attttggttg taggtttacc tttagattgg ggacaattta acgtaggaga
     4681 caccacactg gctgtcacac agctagtagg tgcagttatt tttgctttag ctagtatcac
     4741 agactggtta gatggaaaaa ttgcccgtgc gcgaggcctt gtcactaatt ttggcaagtt
     4801 tgcggacccg ttggcagata aaatgctagt tatgactgct tttatcatgt tggttgacca
     4861 aggaaaggcc cctggttggg tagtagcgat cattgtctgt cgtgaattag ccgtcacagg
     4921 cttgcgttta ctacttgtcg aacatggaga agtcatggct gcagcttggc cgggaaaagt
     4981 caagacggct acacaaatgg ttgctattat cttgctgttt atcaataata tcccattcag
     5041 cctgatcggt attccagtag atcaaatcat gctttatgct tgtctgatct ttactatcta
     5101 ttcaggtgtg gattacttca tcaaaaacag cggtgtgttc aaaggatcaa tgtaagtatc
     5161 gtttagtgga gaggttgtga gcctgccgtc taactctgag tcacaaggga taatttcaaa
     5221 aaacagcttt tcatgtttta tgtatattgt gacttatgac cgaggttttg gcaggagaac
     5281 actgtttatt tggaacaagg agctgtgaca agatttttgt cacagctcct tgccctcttt
     5341 ttaaataaaa aaatataaaa atacagcgta tactatataa gggagcggga agtaatgaaa
     5401 gcagaaataa tagctgtcgg aacagaactt ttactaggac aagtcgtgaa tacgaatgcc
     5461 actttcttgt cagaacagct agctgatcta ggtatcgaag tttattatca aacagttgta
     5521 ggagataatc agcagcgttt agaagagttg attgctctag ctgaaacaag aagtgaactc
     5581 atactcttat gtggcggatt aggaccgaca gaagatgacc tgacaaaaga agcaactgcg
     5641 gctcacctcg gtaagtctct catacaaaat acggaaggat acaaaaaatt attggcatat
     5701 tttgaaacaa cccatcgaaa aatgaccaaa aacaatctgc aacagtccca gattatcgaa
     5761 ggcggggttc ctctaccaaa tcgtacaggg cttgctttag ggacttttta tcaaacagat
     5821 acacatgcgt atatccttct tcccggtccg cctaatgagt tgaaaccaat gtttgtggaa
     5881 caagtacgtc ctttactaga ggaacgtttt ccttctgaag aaaaactaat ttctaaagtt
     5941 ttgcgctttt atgggattgg tgaatcgcga ctggtcactg agctaaaaga tctgattgag
     6001 acacagatca atccaacgat cgcaccatat gccaaaccaa atgaagtgac attacgactg
     6061 acagtgaaaa caaatgatgt tcaggcaggg aatcaagctc ttttagcatt agaagaaaaa
     6121 atccaagaac gagtagggga atatttttat ggatacggcg atgataattc gctagcaaaa
     6181 gtcgtagtgg aactactgaa agaaaacaag cagactgtaa cagcagcgga aagtttgaca
     6241 gccggcgcct tccaagccgc attaggcgac attgctggtg tatctgaagt atttccaggc
     6301 ggattcgtta cctactcgct ccaaacaaaa gctggttttc tggagattga tccggagcta
     6361 ttagcagaat atggaacagt cagcaaagaa tgtgtggagc agatggccat tcaagcacgt
     6421 gagatagcaa gaacagatta cagtgtagct ttcaccggag tggctggacc ggacacgtta
     6481 gaaggtcaat ctgcaggaac agtatggatc gctatttcat ctaaagacgg cgtggtttcg
     6541 gaaaaatatc actttgccag agatcgtgct gctatcagat ataatgcggt tatgcaagga
     6601 tttgatcttt tgagaaaaca gcttttaaga aaatgataaa tgcgaatatt tgttcggttt
     6661 tttcttgctt tttttcacta aaaaagatat gataaggata atgaaatgtt gctctgattt
     6721 gtaaatataa ggaggattat cgtattggca gatgatcgta aagcagcctt agatgctgca
     6781 ctgaaaaaaa ttgaaaagag ctatggtaaa ggctctatta tgaaattggg cgaaaaaatc
     6841 gaccaacaaa tctctacaat tccaagtggc tctcttgcgt tagatgtcgc tttaggtgtc
     6901 ggcggttacc cccgtggacg tatcatcgaa gtatatggac ctgaaagttc aggtaaaaca
     6961 acagttgcac tacacgctat tgcagaagta caaaaaaatg gcggaacggc cgctttcatt
     7021 gatgctgagc atgcgttaga tccgcaatat gcacaaaaat taggtgtgaa tatcgatgaa
     7081 ctacttcttt cacagcctga cacaggagaa caaggtctag agatcgctga tgctttagta
     7141 tcaagtgggg ctgtagatat cgtagtagtc gattcagttg ctgctttagt tccacgagca
     7201 gaaatcgacg gcgaaatggg tgactcacat gtcgggttac aagcacgttt gatgtctcaa
     7261 gcattgcgta aactctctgg ttcgatcaac aaaacaaaaa caatcgctat tttcatcaac
     7321 caaatccgtg aaaaagtagg cgtgatgttt gggaatccag aaatcactcc tggaggacgc
     7381 gcattgaaat tctacgcaac gatccgtttg gaagtacgtc gtgcagaaca attgaaacaa
     7441 ggtacagata ttgttggtaa ccgtacaaag atcaaagtcg taaaaaacaa agtagcacct
     7501 cctttcaaaa tcgctgaagt agatgtcatg tatggtttag ggatctcaca agaaggagaa
     7561 cttctagaca tggcagtaga aaaagacatt gttgataaaa gcggtgcttg gtattcttat
     7621 aaagaagatc ggattggtca aggacgggaa aacgctaaaa tctatatggc taatcatcct
     7681 gaaatgatgg cagaagtttc tgctctagtt agagcagctt atggtattgg tgaagaagtc
     7741 gctgtcccag aagatgaaaa aggacaagaa gaattgcctt tagtagaaga ataaaaatta
     7801 tgataaacaa aaacggctgg tctgaagagt ag
//
LOCUS       DAYYAG010000124         7811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000124 DAYYAG010000000
VERSION     DAYYAG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.127"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACHARQ_002419"
     CDS             complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACHARQ_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8410810.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1383..1901)
                     /locus_tag="ACHARQ_002420"
     CDS             complement(1383..1901)
                     /locus_tag="ACHARQ_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410811.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1917..2243)
                     /locus_tag="ACHARQ_002421"
     CDS             complement(1917..2243)
                     /locus_tag="ACHARQ_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8410812.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2256..4235)
                     /locus_tag="ACHARQ_002422"
     CDS             complement(2256..4235)
                     /locus_tag="ACHARQ_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8410813.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4256..4570)
                     /locus_tag="ACHARQ_002423"
     CDS             complement(4256..4570)
                     /locus_tag="ACHARQ_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8410814.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4738..5031
                     /locus_tag="ACHARQ_002424"
     CDS             4738..5031
                     /locus_tag="ACHARQ_002424"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8410815.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5109..6221)
                     /locus_tag="ACHARQ_002425"
     CDS             complement(5109..6221)
                     /locus_tag="ACHARQ_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8410816.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6374..7222
                     /locus_tag="ACHARQ_002426"
                     /pseudo
     CDS             6374..7222
                     /locus_tag="ACHARQ_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7575..>7811)
                     /locus_tag="ACHARQ_002427"
     CDS             complement(7575..>7811)
                     /locus_tag="ACHARQ_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8410817.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2500 t
ORIGIN      
        1 tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc
       61 tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca
      121 gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc
      181 actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg
      241 tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa
      301 gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc
      361 attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga
      421 tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt
      481 tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc
      541 catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc
      601 tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt
      661 caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc
      721 aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc
      781 agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct
      841 cttgacacac atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat
      901 tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc
      961 tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat
     1021 acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt
     1081 ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga
     1141 taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt
     1201 tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa
     1261 catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac
     1321 gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa
     1381 cattagttat tctcctttcc taaatataac gctttttcat attgtttaat acggatatac
     1441 tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa
     1501 ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa
     1561 ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca
     1621 ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc
     1681 tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt
     1741 cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag
     1801 gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc
     1861 tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat
     1921 ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc
     1981 atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca
     2041 tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt
     2101 cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt
     2161 atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt
     2221 tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta
     2281 ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca
     2341 actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc
     2401 gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc
     2461 aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact
     2521 gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct
     2581 cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct
     2641 tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa
     2701 atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt
     2761 atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg
     2821 acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg
     2881 tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta
     2941 atatttttac cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc
     3001 gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc
     3061 atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc
     3121 ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc
     3181 tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac
     3241 aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc
     3301 tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt
     3361 tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga
     3421 aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct
     3481 actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc
     3541 cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt
     3601 aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct
     3661 gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa
     3721 aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga
     3781 ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg
     3841 tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga
     3901 atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa
     3961 aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca
     4021 aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta
     4081 cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca
     4141 gataataaca attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc
     4201 aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata
     4261 tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt
     4321 ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca
     4381 aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat
     4441 tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga
     4501 aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg
     4561 cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt
     4621 tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa
     4681 taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg
     4741 agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa
     4801 gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat
     4861 gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat
     4921 gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt
     4981 aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca
     5041 catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc
     5101 gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc
     5161 accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt
     5221 actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa
     5281 actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc
     5341 cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca
     5401 atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca
     5461 atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca
     5521 acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac
     5581 atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt
     5641 ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta
     5701 aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag
     5761 ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt
     5821 agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt
     5881 gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa
     5941 ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta
     6001 tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg
     6061 aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct
     6121 tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc
     6181 tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata
     6241 ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt
     6301 tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag
     6361 aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt
     6421 gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc
     6481 agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa
     6541 taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata
     6601 taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg
     6661 aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt
     6721 cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag
     6781 ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc
     6841 aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt
     6901 tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag
     6961 aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc
     7021 ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac
     7081 atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca
     7141 aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt
     7201 gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct
     7261 gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa
     7321 acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta
     7381 ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta
     7441 ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa
     7501 atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa
     7561 ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt
     7621 agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga
     7681 tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt
     7741 ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact
     7801 ggtgtcgcat c
//
LOCUS       DAYYAG010000125         7637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000125 DAYYAG010000000
VERSION     DAYYAG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.128"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..575)
                     /locus_tag="ACHARQ_002428"
     CDS             complement(63..575)
                     /locus_tag="ACHARQ_002428"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8410818.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(589..2886)
                     /gene="recJ"
                     /locus_tag="ACHARQ_002429"
     CDS             complement(589..2886)
                     /gene="recJ"
                     /locus_tag="ACHARQ_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8410819.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3028..3471)
                     /locus_tag="ACHARQ_002430"
     CDS             complement(3028..3471)
                     /locus_tag="ACHARQ_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8410820.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3472..4260)
                     /locus_tag="ACHARQ_002431"
     CDS             complement(3472..4260)
                     /locus_tag="ACHARQ_002431"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8410821.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4257..5198)
                     /gene="rnz"
                     /locus_tag="ACHARQ_002432"
     CDS             complement(4257..5198)
                     /gene="rnz"
                     /locus_tag="ACHARQ_002432"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8410822.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5375..5962)
                     /locus_tag="ACHARQ_002433"
     CDS             complement(5375..5962)
                     /locus_tag="ACHARQ_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8410823.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6183..7496)
                     /gene="obgE"
                     /locus_tag="ACHARQ_002434"
     CDS             complement(6183..7496)
                     /gene="obgE"
                     /locus_tag="ACHARQ_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8410824.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         2053 a         1792 c         1305 g         2487 t
ORIGIN      
        1 ttgagggtcg ctttaaaatc gcttctttaa gaataacatc aagaatagct tcttttttct
       61 gcttaatatt ccattaatgt aatgatgtca tatcctttga ttttgtcgcg tccatggaga
      121 tccatcaatt cgatcaaaaa tgcgcagcct acgactacac cgcctaattg ttcgatcagt
      181 tcgatcgtcg ctttgattgt tcctccagta gctagcaaat cgtcacaaat cagcacacgt
      241 tgacctggtt taatggcatc cttatgcaat gtcaatgtat ctgttccata ttctaatcca
      301 taagtcactt cgatggtttc tcgaggtaac ttcccttttt tgcggactgg ggcaaaaccg
      361 actcctaact cataagcaac aggacatcct acgatgaatc cacgggcttc tggcccaact
      421 accatatcaa ttcgtttttc ttttgcataa tttacgattt gtttagtagc ttcccgataa
      481 gcgtcaccat ctgccatcag tggggaaata tctcgaaaca caattccttt tgacggatag
      541 tcgggaatac tggcaatata atcttttaaa tccactcttt gtttcctcct aggttgttaa
      601 ccatttcttt atggtaggaa tatcgctcaa aagcaaaaat tcctcaactt tgattttatt
      661 gagccgtcgc tggtataatc tgctctcagt caaaggatga ctttctgggc tagcgacttt
      721 ttgtaaaaca ccgttctcta ttgtaacaaa tttcaattca aaaaacacct gtatcataaa
      781 aattaataat ttttcaggga tacgcaaata ttgagctatt gcttttattt tatgacgtat
      841 atcaattcgt tcttgagaat ggatcaattt aaacaatttt gcgtactgtt ctcttgtgcc
      901 taccccattc aaataggcct catcaggtga aatccccatc agataaacac gattgaatga
      961 ataagaccga aaaacttctt tcagatccgc taaatcatct ggacaatcca taacaactag
     1021 agagtttggt tttctttttt caattattgc ctttacagaa tctagtcctt cgaaaacaat
     1081 cgcagcttcc tggataatct caggcacgaa tttcaacgtc gaagaatcaa atgctaaata
     1141 aaggctacct tccgacgttc ggctctgttc acggaaacgt tttgcccgcc aatcaaatac
     1201 ttgcagacca cccacagaaa aatcagatac catcagctgc ggtttcttac gcccgttcca
     1261 ttcattgatc gaaagctttc ctaccacatc tacctgatta ttcagcagtt cttcttcttg
     1321 agtaccaaaa ccaaaagcga ccgcatcaag ctgactcgat gcatcggaaa ggacaaattt
     1381 cagatgctgt tggtttgctc cgattctttt gatattttct gcttggactt gtcgaaataa
     1441 aaactgaggc aaaggattat ccgtaccaaa tggcgccaat agtttcagct catcaattcg
     1501 ctctactgtt acttcatttg gcagcaatac ttcatcaatt ctcaactcag gccctctcat
     1561 caagtcaatt tgatgatcga ctatatattg gttcatcttc tcctgtagta tagtgacatt
     1621 ttcacttggc atcgttaaac cgactgctgc atgatgtcct ccgaagtaag taaataaatc
     1681 tcgcatctgg tcaagcattt caaaaagatt caaggcttca atactacgtc cagatccttt
     1741 agctgatcca tcttctttta aagtaaggac aagcgttggt ttacccgttt cattcattat
     1801 tttcccagca acaatgccca aaacgccttc atgccatcct cggtttacca atagatggat
     1861 atgattctcc ttattgacca tcgctaatgc ttcttgtgtt atctgctcta cgattgcttt
     1921 tctttcttca ttgatctcat tcaatttttt tgcttgaacg gatgcttcct cttcatcaaa
     1981 cgtcgcaagt aacgagacgg caggacgtgg atcgcccatc cgaccgattg catttagtct
     2041 gggagcaatc gaaaaaccga tcgttgtttc gtctgcgtcc ctcattttta cgccgctttc
     2101 ttcaaatagc gcctgaagtc ctatccgttc tgaatgatga atggcatcga ttcctaatga
     2161 aacaaggatt cgattctcat cagtcaatga aactaaatcg gcgatcgttc ctatcgctac
     2221 taaatccaaa aattctgcag gcggttcttc caataatgca gaagctactt tgaatgctac
     2281 tccgactcct gccaaatctg gaaatggata gtgccccgct ggatgacgag ggtgaacaat
     2341 cgcataagcg tctggtaaat tctcaggtag ttcgtgatga tctgtaatga tcacgtctac
     2401 accagcttgc tgagcgtatg ctaccgcatc atttcctgct accccgttat caacagtgat
     2461 gatcaactgg attccttctt cgatcttttc ttggtatacc gattggtttg gtccataccc
     2521 atgctcaaat cgatttggca ggaatacttc tacttctgca ccaagtaatt ccagtgtttc
     2581 tttcattacc gttgcactgg tgatcccatc tgcgtcataa tcaccgtaaa cgaggatttt
     2641 ttctccactg attacagctt cttggattcg gctgattgct ttatccatat cgtgtatcag
     2701 atacggatca tgtaattctt gttcctttgg atgtaaaaat cggttgatat ccttttcctc
     2761 atggtaacct ctttgccaca gtaacttacc gacaaatgga gataatttca gttcacggat
     2821 catttggaga aaggattcag aaggctctgt ctcgcttaac ttccaatcaa aatttgcttg
     2881 tctcactttc tcactcctaa cttagttatt atagcaaagg aaatcagcaa aaaaaagcag
     2941 aaagaaagaa taaaaacaga tcagatccgc tagacagatg ttcagcagga cctgatcttt
     3001 tgtacgacca tttatgaaat atccctatta gtcaaaataa ttgaagcttt gtttgggcga
     3061 ttgagttttt tgttcttccg gcgctttcaa ttgactgatt tgtgcggttt tcgcttgtaa
     3121 ctccgcctga taagcagctt ccaactccgc acgttcgttt ctaaactcgc gttgctgttc
     3181 ttcttttgct tcttcaattt tcttttgaag ttctgtttga tattctgaca tttcctgagt
     3241 cagctgtttg atttgctttt tttgctgcca tagtgttgcc gaagcagtca aaaatacaat
     3301 cagtgcacca atcagcgcag aaccgatgat cactaagatc aatggcgcag aaaaggaagt
     3361 gaagccaaaa cttacaggaa catttttatt gttcaataca gcaaaaagaa caataatcaa
     3421 aactaaaaca aatccaagaa tcacattgct ttgctttttc aattccttca cctacttttt
     3481 gttaaaaatt ccgctggcta aataatcacc aatcgttgga aacagcgtat aaaattttga
     3541 tgcaacttct aaaataaacg gttgattgat ttcccgtttt ggatgtctca tattgcggac
     3601 gatcattttt gccaacttgg caggttctaa aacgatttgt ccgacttttt ccaaatagct
     3661 tccgcttgga tcagccttat cgaagaaatt tgtctcgatt ggacctggat tcactgtcgt
     3721 aacagctacg ccaagtggtt tcaattctaa tcgcaaagcg ttggagaagc caaggactgc
     3781 aaatttcgtg gcagaataaa ccgttgattt tgcagttgcc atttttccag ccatagaggc
     3841 gatattaatg atatgacctt gtcttctttc tgccatatcg atcgcaaatt tttgtgtcaa
     3901 aaccatcatt cctaagatgt tgacttcaaa catatcgtat gcttttccaa ggtcgaatgt
     3961 aaggaagtct tcgaaaatcc caaaacccgc attgtttacc agaatatcga cgcgcccgac
     4021 ttcttccatc accttttcat ataattcgtc cacgctttca ggattgctga cgtctacctt
     4081 gaaagcataa gcctctttgc cgctcaagcg tctgcactcg tctctgacac cttctacaaa
     4141 tgccattctt cgtgcgcaac ttataacgat tgctccttgc tttgctgctt cataacagat
     4201 ttgtgcgccc agaccgcctg aactacctgt tactaaaaca actttatctt ctaaattcat
     4261 gcctcttcct cctcgcgaaa tggaatctca atgctatcaa aatctttcat aatcgtcgtg
     4321 ttttcaaaca cttcttgtgc ttctttttca agctctaaag ctgcttttcc taaatatctc
     4381 gcactgatat gagtaagtag cagccgtttt gcctgcgctt cttttgcaac ttcagcagct
     4441 tgatgagtcg tggaatggaa atagttgtgc gccattcttg cttcatcttt attaaatgtg
     4501 ctttcatgta ccagaatatc gctattttcc gcaagcacaa cggaattatg tgtttttctc
     4561 gtatcgccta aaatagtcac gattcgtcct tttttatctg gacctacgaa atcttttcca
     4621 ttgatcgtcc gcccatcttc tagttggatc gtttcacctt gcttcagttt gccgtacagt
     4681 ggtccagctg gaatacctaa ttcttttaga cgatccacct gcaattctcc tttatgatca
     4741 tgttcaacca cacgataacc aaaactgtcg atcccatgat ttagtttttt tgtataaaca
     4801 gaaaattgct gatcagtaaa aatcggggct gtttcattta gctcaatgaa tttcagcgga
     4861 taacttaaac gcgtttgcga aatacctaaa gagactttta tgtattcctc tatccctttt
     4921 ggcccgtaaa tctctaatgg cgtatcccca ccttgaaatg accggctgct gatgagcccg
     4981 ggaagtccaa aaatatgatc gccgtgtaaa tgtgtaatga aaattttacc gatttttctc
     5041 ggacgaatag tcgttcgcaa aatctgcatt tgcgtgcctt cgccacagtc gaacaaccaa
     5101 acttcattgc gctcgtctaa aagtttcaat gctatacttg tcacatttct atgttttgca
     5161 ggaacacctg cacctgttcc taaaaattgt aattccataa tctacctgac tttctttaag
     5221 ttcctcttta attttagggt aaaaagaagg gataatcaac cgtaagtcga gaatgggttc
     5281 aaattaaaaa taacgttcta aagcgatagc gttttctttg aaaattacta taaaaacagc
     5341 agcgaacccg tttgagatcc tctgctgttt tttattaatt tgaagatcca gaactttcat
     5401 tgtcgttgga agtttgcgga tgtttgtcct tccaagtctt ttgtgcagct tctttcgcat
     5461 gattttcagc ttctttcgca tcatgcacat ctttttcgac atcttcataa tccaagcgag
     5521 taatttctcc gcctaattcc atcattacca attgattcag tgggcgattg tcctcttcat
     5581 cagcaatcaa gatcagtcct gtagctcctt caccgatttt atttcctaca aagtcgaaca
     5641 cggtctgggc atcttttatt tctttggcat cttgtgtcgc accgatcatt cctccggcaa
     5701 accatcctaa taaaatccct agcggaccac ctaatacacc aaccaacatt ccgattaatc
     5761 cacccgtaga agatttgtta ttaccggtaa agtcgataaa atcttctatt ttgaattgat
     5821 gtgtcccatt aggatcatgg ctgacaactg ccatctgttc tcctttaatc gaacgaacag
     5881 catgtaactt tttgatttct gaaaatgcct gatatgcctg gctttctact tgaaaattta
     5941 aaatgattac tcgtttgctc attaaaaacg cccctttcat accttcttgt tatttatttt
     6001 acctttaatt gaaaaataag aaaagaaaag cactcttttt ttccaataaa atagggctgt
     6061 gacaaaagtc gtatcacagc tcctttttcc gaataaacgg cgttcttgaa gctgatcact
     6121 tctgtcacaa cctcccttaa atctgaatct atattttttg gtatttgttt ttatttgctt
     6181 gcctattcga caaattcaaa ttcaaactca ccgatacgta caaggtcgcc atctttcgca
     6241 ccgcgtgccc gaagagcttc atccacaccc ataccacgta attgacgagc aaaacgcatc
     6301 actgtttcat catgatcgaa attcgtcatt tggaataatt tttcgatctt ctcaccagat
     6361 aagatccacg ttgcatctga atcacgatcg atttggaact caggaccttc tggattgaat
     6421 ccatagtgta cagtttcttc ttccagttct tcttcgtaca atgggaattc tggcgttact
     6481 tctaacaaat ctgctgtagc attcaataac gcgtccaatc cttgtcgagt cactccagaa
     6541 attggaaata ctgggatgtc atcagcaaat tcatcttctt tttccttgtt cagctgttct
     6601 ttgaatttca ctagattttc cggtgcatcc ggcatatcca ttttgtttgc cacgatgatt
     6661 tgcggacgct ctagcaagcg taagttataa gtagataatt ctttgttgat ggcaagatag
     6721 tcttcataag gatctcgtcc ttccatccca ctcatatcga tcacatgtaa aattactcgt
     6781 gttctttcga tatgacgaag gaattgtgta cccagcccaa caccttgcga agctccttca
     6841 atcaagcctg gcaagtctgc tgcggcaaaa cttctgccat cacttgtagt aaccattcct
     6901 agattaggta cgagcgtcgt gaaatgataa gcgccgattt ttggacgagc agatgaaata
     6961 acggatagta aagtcgattt cccgacagat ggaaaaccta caagtccaac gtctgccaat
     7021 actttcaatt ctagttcgat ttttcgctct tgtcctggtt caccgttttc agcaatttcc
     7081 ggtgcaggat tccgaggaga ggcaaaacgg atattccctc gtccaccgcg tccacctttt
     7141 gccacaacta gtgtttgacc attttcaatt aaatctccta aaagagcacc agtctccgca
     7201 tctcggacag tggttccttg tggtacttta acataggtat cttctgaacc acgaccgtgc
     7261 atccctttac tcatcccatt ttcacctggc tgcgctttga aatggcgatt gaaccgaaaa
     7321 tccatcaatg tacgtaaccc ttcatcgacg atcaagatga catcgccgcc acgaccaccg
     7381 tctccgccag ccggtccgcc gtcaggaacg tatttttcgc gacgaaatgc aaccattccg
     7441 tctccgccct tgccagcttt cacatcgatc gttacttgat ctaaaaacat ggacataatt
     7501 aagtcctcct attattcatt tatttatttg actgcctgaa tttccacaat aaacaaaagt
     7561 cttacttatt ttatcggtga aatctgattt tgtctatcat ttcagcaaaa agattgttta
     7621 ccttcttctt tttttat
//
LOCUS       DAYYAG010000126         7585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000126 DAYYAG010000000
VERSION     DAYYAG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.129"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..847
                     /locus_tag="ACHARQ_002435"
     CDS             <1..847
                     /locus_tag="ACHARQ_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8410825.1"
                     /translation="KYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDYAKVNRLLQIRS
                     DVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIPNYLPENEIKHY
                     LNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMRLDSEICDMFKL
                     EESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERVCRICGGKASKN
                     PYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLLDEFAHDSFGIN
                     LKRRK"
     gene            849..1166
                     /locus_tag="ACHARQ_002436"
     CDS             849..1166
                     /locus_tag="ACHARQ_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410826.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            1293..1916
                     /locus_tag="ACHARQ_002437"
     CDS             1293..1916
                     /locus_tag="ACHARQ_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8410827.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            1931..2821
                     /locus_tag="ACHARQ_002438"
     CDS             1931..2821
                     /locus_tag="ACHARQ_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8410828.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            2844..3059
                     /locus_tag="ACHARQ_002439"
     CDS             2844..3059
                     /locus_tag="ACHARQ_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410829.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            3071..3412
                     /locus_tag="ACHARQ_002440"
     CDS             3071..3412
                     /locus_tag="ACHARQ_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410830.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            3412..3783
                     /locus_tag="ACHARQ_002441"
     CDS             3412..3783
                     /locus_tag="ACHARQ_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410831.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            3776..4171
                     /locus_tag="ACHARQ_002442"
     CDS             3776..4171
                     /locus_tag="ACHARQ_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8410832.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            4173..4550
                     /locus_tag="ACHARQ_002443"
     CDS             4173..4550
                     /locus_tag="ACHARQ_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8410833.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            4551..5159
                     /locus_tag="ACHARQ_002444"
     CDS             4551..5159
                     /locus_tag="ACHARQ_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8410834.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            5159..5500
                     /locus_tag="ACHARQ_002445"
     CDS             5159..5500
                     /locus_tag="ACHARQ_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410835.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            5742..7585
                     /locus_tag="ACHARQ_002446"
                     /pseudo
     CDS             5742..7585
                     /locus_tag="ACHARQ_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         2761 a         1002 c         1540 g         2282 t
ORIGIN      
        1 aaaatatcgt gaatatgtgg aaaaaaatta taagtcatta aaaatgtatg atgcaaaata
       61 caaagagttt atcgatgaat tttttccacc atttgactat gcaaaagtca atcgcttatt
      121 acaaatacga tcagatgtat ttaaaattct tgcagaatat gcgatggatg cagatgtgaa
      181 tcaatatttt tctgatcgct tagaggaaat tcttcaaaga acatattctt ctaatgctaa
      241 tgtttttgtt caacttttaa acgttgatat accaaattat ttaccagaaa atgaaattaa
      301 acattatttg aactatccat ggtcaggaaa gacattttca agaagacttt ggggaaatat
      361 ttcatcgctt gaacagaaac tctctaatgc tattgtaaaa agtgttgcta gtggagaagg
      421 cgttatacac gcattaaaca ccatgagatt agactcagaa atttgtgaca tgtttaagtt
      481 agaagaatca aagtataaca aagcgataga aaatctcgtt cgaacggagt atgcaaaatt
      541 tgcacaagat ggtattgaaa aatcatattt agaaacaggt attgaggaat acaacgtatt
      601 gactgcaaaa gatgagagag tttgccggat ttgtggagga aaggcaagta agaatcccta
      661 taaactgaaa gatgctgtca taggtgaaaa tcgagcacct ttccatagtc gttgtagatg
      721 tacggatgtt cctaatttac caaaattagg aaaggatatt gatgaagaat atgaccgttt
      781 atttggcgat ttattagatg agtttgcaca tgattctttt ggaattaatt tgaaacggag
      841 gaagtagaat gaaagatttt tttgaagcag tactaacaat taatgtaaat gctgatattg
      901 cagaagccta caaaacagct attgagtctg agaaccatcc taatggcttg agagaccatt
      961 ggaatggcaa ttatgcctac gtggttattg gcgatcaaac tgttaattat caagataata
     1021 ctccagttga taagaatacc gttaatttaa cgattcaatt attatctcat tcattaccaa
     1081 atttaaaaga aacagttgat tggtatgaaa agatgggatg tattgttgtt agaactgact
     1141 acaaagaagg aaagtctagt aattaggctt ttttattttg tccgaaatga cgttaaacta
     1201 gcgcaatact gggcttggtt gaatggtggg gcgcaactat taaaactcaa agcaatgcgg
     1261 ggcgtgaaaa cgaatcgtgg ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc
     1321 caatgaattt gcaatacttt gccgaaggtg acgatcaaaa gttttctttt gatgacttca
     1381 aatcttttgt ggaatccaat gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg
     1441 ttgccgacaa acaattggaa gcttggaaac aaaataactt agataaaatt aaacaggata
     1501 ccatcaagga atatgaggaa tctaagaaaa acaagtcacc tgagcaaatt caactggaaa
     1561 aactacaagc tgaatttgaa gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat
     1621 ttgttgcaga acaaattgct ggattagaac tagatggaga gttaaaagag tctatttctc
     1681 aatttatgct aaataatctt gttagttcgg atacagattt cactaagaat gctgttgaag
     1741 gttttacaag tgttttgaat gcaattaatg agaaacatgc agatgcatta aaagaactac
     1801 aaatgaagtc tgcatttggt ggaactcaac aatcgaataa ccaggttcag cagaacaatg
     1861 aaacattaac aaatccagaa gaacaattag gacaaattct tcaacaattt aactaggaga
     1921 gtgaaaaatt atgaaaaaaa catctttaaa taatttagag tatttggata tttcaccagc
     1981 gattaatgct atgcaagtac caaatacacc ttttttaagc tatttacttg gtgctggaaa
     2041 aacagagcca gcaaactcga cagaaattaa atggcgtgaa tatgatatca acaacgatga
     2101 ttcttctgaa aaacttgagg gcggagaata tccagatgct gaatcaggtc gaacttggtt
     2161 taacaactat actgaaattt ttagaaaatc aacctctgta tctggtacat tagatgctat
     2221 taatgtgaat ggagtcggaa atgaattaac taatcaagta gcactacgtg gtatggaaat
     2281 gaaaattgat ttgaaccgaa aattgattac tggtgtaaaa gctgatgaaa atggttctaa
     2341 aggtcgtcga atgaatggga ttttgaactt gatcaattca gcaaataagg cagaaacagc
     2401 cactgcgggt gcagtaacaa gaaaagatat cgatacttta tttaaattga tgtatgaaaa
     2461 aggttatatg ggagaaaaac tatgcttgat ttctccagat atgcaggagt taatgactga
     2521 tgagttagat ggaaaatcaa caaaaattgt tcagttcggg gaaagagtaa cttttggatt
     2581 gcaaattgga aatatcgtgt ctaattacgg tacaggtatt gctctactag aaccatcatt
     2641 gccaaaagga acaattgccg caatcgatac taattatgtg aaactacgtc cattacgtga
     2701 atggagagca gaagaacttg caaaaacaac ggattctaga cgtatcggtc ttgtaggtga
     2761 atactctctt gaatacaatg cttcaaactc tggggcaatt ttaaatttaa agtctgaata
     2821 aaagggagtt agtactccct tttttgatag gaggaattac agtggtaaaa aaagatgaaa
     2881 ctaaaaaaga tgaagtcgtg aaatatagag taggtaaaac taaaaatttt gttgggtttg
     2941 ttcatcctaa aactcgtaga tttatcacag cagattcaaa taacgaattt atcatttcta
     3001 tagatgataa agaagcaatt gcaattttgg aagatgcaat tgatgttaat gaaatttagg
     3061 aagtgatctg atggatgaat cgctaaaaac ggaaatcatt gagtctacaa aagaagattt
     3121 tccagatttg agtgaagaac gcataactaa tttattagaa ataattttgc tagaaattga
     3181 atcatacaac acttgtaaaa atgatatttc atgggaaaag ttaaaaagcg tgattagtga
     3241 agtgttgtat aaaataataa aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg
     3301 cgatacaacg attagttatg cttcaacaac aaatgatgtt agtgagttac ttctaggcta
     3361 tggtgatttg atacgaagag ttattggttg tggaggattg gagttttttt aatgaatgaa
     3421 gcagatattt tagaaactac ttatgaagat agttgtatta ttgaaagact gacggacatt
     3481 gaagattcta atacaaatat tactattcaa gattacaaaa aagtatatga taatcctatt
     3541 tcttgcgctc tttcacaagg tcaaatcgat ggactagcag tcatagaaga tggagagatg
     3601 gtaaatgttt caactgacac atataaatta tttgttcatc ctaagattaa actaaaaaaa
     3661 ggagatcgaa taacaataac tcaaaaggcc agtggcttaa ttttttctct atttgctact
     3721 aagccttttt actatcctag tcattgtgaa gtgaatttga taggaagtga gaaaaatggg
     3781 taatctcaaa tatgaatcaa atgcagaaaa gatcattgag aattttaaaa atatgactgt
     3841 aattgctcaa aaagaaggaa tatccttcgt taacgattca atgaataaag tcgttagttt
     3901 agctaaacca ttaactcctg taaaatcagg taatttaagg cgaggatatc gtgtagtaaa
     3961 agctagaaaa ctatcaagcg gtcgaattgt tggagcagtt ataaacaatg aacattattt
     4021 taaatatgta gaagaaggtc ataggactaa aaatggtggc tttgtcaaag ggaaattcat
     4081 gttaactcgt gcaacgaatc ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat
     4141 ggcaataaaa atcgttaaga aaggaaagta acatgaaaga tgaaatcatt gctgcaatca
     4201 gtagcaaatt aaaagaaatc tatcctgatg ggacaatata tcttgattca gttatgcagt
     4261 caactaaaga tttttatttc gttttatcag taatggaatc tggaactgaa aatgtaggaa
     4321 ttgatattca aaatgtttct ttcttaattg atattgcatt gattgataat aaacctaata
     4381 gaaatttaat taatgaatta gtctcacgtt gtgggacttt ttttaatacg attacaatcg
     4441 atgagcaaat attatttcca aaagcctatt tacctgatga agcagatggt gttcaacata
     4501 ttcgttttac attagaattt ccacaatata ttgaatggag tgaaagataa atgggagaaa
     4561 aaagaagtaa aaccggaatt atttctgtcg aaaaacctac ttggtttcct ttggaagatg
     4621 agacagggaa ttttccggta tatggtactg cgttgccaat gggaacagca gtaagtatca
     4681 aaccaacagc taattatgaa acaacacaag attatgggga ttcagtggtt caagatcaat
     4741 ttacagcttt tggtggtgca gaagttgaac tggaagcaaa tggatatact cataaagttt
     4801 tatctacaat tactggaggg aaaattgtta aaggtggtgc cttgcgttct ggagaagata
     4861 ttgcgcaaga tggtgcgttt gcatatagac gtaaaaaatc gaatgggaaa taccgttata
     4921 cggtctttta taaaggacag tttgctctag attcagatga aacatcaacg attgaaggaa
     4981 gtaaagtaag ttttactcat ccaacttgga agggttcatt tgtagatgta ccaggacttg
     5041 gatatatgta ttcagttgat gaagatgatg aaggcgtgga taaagcaatg atcggaaatt
     5101 ggtttacaaa agtagcaatt ccaattgaag aagcagaact ttcaggaggt acagaataat
     5161 gtcaaaatat caaacaacga ttaaattaac gaaaaaagat gaagaaggta aatatgagca
     5221 agtacaattt aaatctgctg aatttctacc aggcccagtc gtagaagaag cagcagaagt
     5281 aatggaggga atgcaaaatg ctgtagatgg aaaatcagta tcagaagcat taagtcgagc
     5341 ttattcattt attgcagata ttttgtttga aggacagttt actggtgaag attatcgtca
     5401 agggatcgat gctcgagaaa ttgcttcgtt gacaggtaag ttattaaaat ctgttactgc
     5461 aggctttgat gaaacttata cagaaacgaa aaaaaagtaa gcgaagctct caaatatcct
     5521 ttttttaaac attcgattac ttatcgagaa ttagatttaa agacgcaatt actcgaagca
     5581 ggttggacgt taccagaaat tgaacgtact gactttgacg agttaatgcg tctttttgct
     5641 tttcgagatg cagtaaaaga gcatgaggat gttgagtact acgataactt cacccaattt
     5701 taggaggtga tactttgaat aacgatgacc taattctgaa gatgatacta gatgaatctg
     5761 gttttactgc cggtatgaat aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg
     5821 aaagaaaaag tagaaatagt ggcagttcat taggtagtat ttggaaaata tttgctggaa
     5881 gttttctagc tagcggagta actagaatcg taggagctgg ctttgatcta atcaagggtt
     5941 ccataagtgg agcggttgat cgagtagata cgatgaataa cgctctacga aatttccaaa
     6001 atatgggatt tagtaattca gaaattatga agaatatcgg aaagaatggg cttttatctc
     6061 aaggtattca aggacttcct actgctttga atgatgcgat aagtcatgtt caacttcttg
     6121 cttcttctac aggtgattta actcgttcaa ctcaaatatt taaagcttta aatgatggaa
     6181 ttcttggttt cggtggttca actgatcaag ttaatgaagc tgttattcaa ttatctcaaa
     6241 gtttctcaaa cggaaaagta gacgcacaaa cttggaactc aatgatcaac gctcaacttg
     6301 gacctactct ttctgctatt gctaaaaaga tgggaattac aatgggagat ctgaaagaag
     6361 gtttatctca aggtaagatt tctgttgaag aattccaaaa tcaattaata gaaatggata
     6421 ccaaaggtgg cggaggactt aaatcattaa gtcaaatcgc taaagattct actaagggaa
     6481 ttaaaacctc tatacaaaat gcaaaaacag ctgttacacg tggtgttggt gaggtaatag
     6541 aaggattaaa taaagcatta gtagattcag atttaggtgg atttaaggga attattgata
     6601 aagtgggcag ttcaatggaa tcgttcttaa aagtaattgc tgcaaatatt cctacagcag
     6661 tatcattttt aagtaattta tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca
     6721 tgatgccctt tcttgttcct gcagctaccg cttttggagc gttcatgttt caacttaaag
     6781 gtataccagc aataataaaa agcttcaata attttaagaa tgccataatt ggcgttggaa
     6841 attcattaaa gattatgggg gcaatagctg ctgctaattc atttgtttta attattggtg
     6901 cagtcgttgg tgcaatagct gttttcggtt actttatggc aaccaatgaa gagcttagaa
     6961 acaaggttat atctatttgg aatgatgtga aagattccgt aattggtgta ttaaaaaata
     7021 taaaggactg gggaattgat acttggaatt ctgctaaata aatggcatca aatgcagttg
     7081 agggtgtcaa agatgcttgg tcaggaacaa aggaatggtt ttctaacacc tggaaaggta
     7141 ttaaggatgg ggccacaggt ttatttgata aaacagtaga gacttcaaga aatgcagtcg
     7201 atagtgtaaa aaatgcatgg tctaatatga agaaatggtt ttctgatact tggcaaagta
     7261 taaaagattc agccagagaa aaatggaatg gaatcaaagg ttctattatg tcagttgctg
     7321 gtccattgat tacaggtata aagaatgcat ttttacatgt tactttttat ctagagacct
     7381 tatggaataa tttagttgag attggtaaaa acgtctttga gattttaaaa aatgttatac
     7441 ttgcgccggt tttgtttatt acctccatga tttctggtgg atgggaagaa acaaaaaata
     7501 atatgattgc tgtttggaat aatattaaag aaagtgccat aaatatttgg gaatctataa
     7561 agaatatatt tgtaagttat tttac
//
LOCUS       DAYYAG010000127         7563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000127 DAYYAG010000000
VERSION     DAYYAG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.130"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHARQ_002447"
     CDS             <1..1087
                     /locus_tag="ACHARQ_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8410836.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHARQ_002448"
     CDS             complement(1305..2447)
                     /locus_tag="ACHARQ_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8410837.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHARQ_002449"
     CDS             complement(2452..2637)
                     /locus_tag="ACHARQ_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8410838.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACHARQ_002450"
     CDS             complement(2991..3236)
                     /locus_tag="ACHARQ_002450"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410839.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACHARQ_002451"
     CDS             complement(3230..3631)
                     /locus_tag="ACHARQ_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8410840.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACHARQ_002452"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACHARQ_002452"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8410841.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="ACHARQ_002453"
     CDS             complement(5649..7415)
                     /locus_tag="ACHARQ_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8410842.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         2428 a         1449 c         1035 g         2651 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ata
//
LOCUS       DAYYAG010000128         7477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000128 DAYYAG010000000
VERSION     DAYYAG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.131"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..305)
                     /locus_tag="ACHARQ_002454"
     CDS             complement(15..305)
                     /locus_tag="ACHARQ_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410843.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(423..683)
                     /locus_tag="ACHARQ_002455"
     CDS             complement(423..683)
                     /locus_tag="ACHARQ_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410844.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(820..1233)
                     /locus_tag="ACHARQ_002456"
     CDS             complement(820..1233)
                     /locus_tag="ACHARQ_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8410845.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1689..2174)
                     /locus_tag="ACHARQ_002457"
     CDS             complement(1689..2174)
                     /locus_tag="ACHARQ_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410846.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2313..2528)
                     /locus_tag="ACHARQ_002458"
     CDS             complement(2313..2528)
                     /locus_tag="ACHARQ_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8410847.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2752..3552
                     /locus_tag="ACHARQ_002459"
     CDS             2752..3552
                     /locus_tag="ACHARQ_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8410848.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3571..5439
                     /locus_tag="ACHARQ_002460"
     CDS             3571..5439
                     /locus_tag="ACHARQ_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8410849.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5432..5923
                     /locus_tag="ACHARQ_002461"
     CDS             5432..5923
                     /locus_tag="ACHARQ_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8410850.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5911..6465
                     /locus_tag="ACHARQ_002462"
     CDS             5911..6465
                     /locus_tag="ACHARQ_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8410851.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6483..>7477
                     /locus_tag="ACHARQ_002463"
     CDS             6483..>7477
                     /locus_tag="ACHARQ_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8410852.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2657 a         1200 c         1423 g         2197 t
ORIGIN      
        1 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
       61 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      121 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      181 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      241 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      301 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      361 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      421 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      481 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      541 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      601 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      661 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      721 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      781 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      841 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
      901 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
      961 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1021 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1081 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1141 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1201 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1261 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1321 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1381 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1441 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1501 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1561 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1621 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1681 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1741 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1801 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1861 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     1921 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     1981 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2041 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2101 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2161 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2221 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2281 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2341 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2401 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2461 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2521 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2581 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2641 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2701 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2761 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2821 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     2881 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     2941 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3001 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3061 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3121 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3181 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3241 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3301 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3361 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3421 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3481 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3541 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3601 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3661 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3721 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3781 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3841 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     3901 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     3961 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4021 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4081 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4141 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4201 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4261 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4321 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4381 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4441 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4501 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4561 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4621 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4681 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4741 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4801 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4861 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     4921 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     4981 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5041 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5101 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5161 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5221 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5281 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5341 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5401 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5461 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5521 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5581 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5641 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5701 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5761 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5821 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     5881 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     5941 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6001 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6061 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6121 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6181 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6241 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6301 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6361 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6421 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6481 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6541 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6601 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6661 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6721 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6781 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6841 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     6901 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     6961 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7021 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7081 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7141 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7201 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7261 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7321 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7381 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7441 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DAYYAG010000129         7468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000129 DAYYAG010000000
VERSION     DAYYAG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.132"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1902
                     /locus_tag="ACHARQ_002464"
     CDS             <1..1902
                     /locus_tag="ACHARQ_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8410853.1"
                     /translation="IHWLFVHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKI
                     FTQRQAPMILTIIIIMGFFFLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPV
                     FPSDLKMLTGLSELLSMVSPVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRF
                     ISIAVLLGCFSGVFFINHKNSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDI
                     KIMEEPAGYSKEKINGIMKKYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNL
                     TVPTDPIPYIKSIITEGTGGLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQL
                     VERQKISPNITDLFDEKIAIHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIE
                     QSPRISDDSAYKETLKALQSNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPN
                     RRKELQTYMQGLHYTDTAVKKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQ
                     HQTDYFIYNNKYSREQAATLNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPA
                     LTIDPTESLSNRFNGKQVFVTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSA
                     TWATQKIKD"
     gene            complement(1962..2678)
                     /locus_tag="ACHARQ_002465"
     CDS             complement(1962..2678)
                     /locus_tag="ACHARQ_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8410854.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2905..3447)
                     /locus_tag="ACHARQ_002466"
     CDS             complement(2905..3447)
                     /locus_tag="ACHARQ_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8410855.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3452..3679)
                     /locus_tag="ACHARQ_002467"
     CDS             complement(3452..3679)
                     /locus_tag="ACHARQ_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8410856.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3758..5638)
                     /locus_tag="ACHARQ_002468"
     CDS             complement(3758..5638)
                     /locus_tag="ACHARQ_002468"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8410857.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5827..6441)
                     /locus_tag="ACHARQ_002469"
     CDS             complement(5827..6441)
                     /locus_tag="ACHARQ_002469"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8410858.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6583..7143
                     /locus_tag="ACHARQ_002470"
     CDS             6583..7143
                     /locus_tag="ACHARQ_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8410859.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2277 a         1494 c         1221 g         2476 t
ORIGIN      
        1 attcattggc tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt
       61 accttcgtgc agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt
      121 gccttaaaaa tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg
      181 ggatttttct tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc
      241 ttgatcatta acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag
      301 cctgtctttc cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt
      361 agcccagtct tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt
      421 attgtacaac gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt
      481 atcagtatcg ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat
      541 tcgccttctt tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa
      601 ggcgccgtaa aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc
      661 atggaagaac ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac
      721 acaaaggcag atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt
      781 ctcaatttaa gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca
      841 gaccctattc cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc
      901 gtcggttatg gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc
      961 agtaacctgt ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata
     1021 agtccaaata ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct
     1081 ctttatcgca gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac
     1141 agtccggata aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat
     1201 tcagcataca aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc
     1261 caattatcca ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac
     1321 actgctgaag gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa
     1381 ggccttcact ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat
     1441 aaaccgatta cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat
     1501 tcgaaaaaat atggattaga acagcaccag acagattatt tcatttataa caacaagtat
     1561 agtcgtgaac aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct
     1621 atggctcttg agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta
     1681 tctaatcatc tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat
     1741 ggaaaacagg tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag
     1801 aaacaaaaag aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac
     1861 cagtacagtg caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg
     1921 ggttgtgata aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa
     1981 acgatttgat taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa
     2041 ttatcgggat tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac
     2101 ccgaatagat agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct
     2161 gtgatttgat agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact
     2221 ccaccaatag ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat
     2281 aagtccacac tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat
     2341 ttggtcataa atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact
     2401 tgttcgctgc tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt
     2461 tgagttgttt cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta
     2521 cttgctgcag ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc
     2581 gctgtttctt ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa
     2641 aaagctagac caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac
     2701 tcccacttct ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc
     2761 atcaatcttt caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat
     2821 cagcgggtgc ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat
     2881 ttgtatttga agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg
     2941 gacagttttt ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag
     3001 ttcaacaagc aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg
     3061 tgtgataaag acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc
     3121 tggtaaatat ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa
     3181 agtagacgga acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct
     3241 cgcttcttga tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa
     3301 aacatacaat tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg
     3361 ccccaccatg gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg
     3421 aacaaatttt tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga
     3481 cttcataacc taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat
     3541 caaagtttgc tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct
     3601 gttgagaaac cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt
     3661 gcataataat ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga
     3721 ttacagcttc cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt
     3781 cgtgtccaaa atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt
     3841 gaacgaacat cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata
     3901 agacgacacc aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca
     3961 agcggtaggc atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt
     4021 cgatagcaac atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg
     4081 gagaatcatt gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt
     4141 tcacaaattg aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca
     4201 tatcagctac ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg
     4261 ctcctgcttg tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca
     4321 cacctatgat ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt
     4381 gttgtaattg atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa
     4441 tcaatttggt gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc
     4501 ccttgattgt ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag
     4561 cataatggat cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta
     4621 aagctaagct gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc
     4681 ctttggtcaa tgtaccagtt ttatcaaaga caaaagtatc aattttggaa aaagtatcca
     4741 ttgcttcacc gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat
     4801 tcgaaacagg cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg
     4861 ctaaacgaaa atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag
     4921 tataatactg agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt
     4981 cttctactaa ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa
     5041 tcgtcacgta gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga
     5101 cgacttgtcg tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga
     5161 ctcgtccgtc cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca
     5221 cgtcatcgat ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg
     5281 gggccatctc ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca
     5341 gatagctacc aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca
     5401 taaaggcacc aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca
     5461 ctgcctgata cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag
     5521 caatgttata gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca
     5581 cagctgataa ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct
     5641 ttctccctct tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa
     5701 aattgaccac tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa
     5761 aaaatagtta gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg
     5821 aaattttcat tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa
     5881 gatttttatc ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg
     5941 atacgtacag ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa
     6001 ttctgttagc aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa
     6061 aatgatctct ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc
     6121 tgtctcgtaa gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt
     6181 aacatatatt tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag
     6241 caaaacacct gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa
     6301 ctccatatac aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct
     6361 ctcaaagttc attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa
     6421 gggtttaaaa gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta
     6481 cattataata gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa
     6541 gcgacagctt tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga
     6601 cgcgtcaaaa aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa
     6661 aagattcaag caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact
     6721 ttttatacac attataagga tatctatgat ctatatgatc aattagaaag caactttttt
     6781 cgggatctaa atgaaattct ctcttatgat ccagcccata gctatgagga actatacact
     6841 cgcttgattg actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat
     6901 ggtgatattt cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata
     6961 tggctatatg aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac
     7021 cacgtccaag gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc
     7081 aaaaaagaat tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct
     7141 tgaataaaaa catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga
     7201 atcttattta gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac
     7261 ttattgaaaa gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac
     7321 caatcaaatt cacctcatca ttaataaatt acttttaggc taatttatta caacttatag
     7381 gttgaagctg taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc
     7441 ttctcgttta cctcaattca ttttcaag
//
LOCUS       DAYYAG010000130         7342 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000130 DAYYAG010000000
VERSION     DAYYAG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.133"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..675
                     /locus_tag="ACHARQ_002471"
     CDS             <1..675
                     /locus_tag="ACHARQ_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8410860.1"
                     /translation="VAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVI
                     ISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYF
                     ASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYN
                     IPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFD
                     SDSALF"
     gene            687..1607
                     /locus_tag="ACHARQ_002472"
     CDS             687..1607
                     /locus_tag="ACHARQ_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8410861.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1648..3105
                     /locus_tag="ACHARQ_002473"
     CDS             1648..3105
                     /locus_tag="ACHARQ_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8410862.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3450..4064
                     /locus_tag="ACHARQ_002474"
     CDS             3450..4064
                     /locus_tag="ACHARQ_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8410863.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4057..5781
                     /locus_tag="ACHARQ_002475"
     CDS             4057..5781
                     /locus_tag="ACHARQ_002475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8410864.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5778..7229
                     /locus_tag="ACHARQ_002476"
     CDS             5778..7229
                     /locus_tag="ACHARQ_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8410865.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2507 a         1222 c         1466 g         2147 t
ORIGIN      
        1 gtagcattta tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat tatgagcgag
       61 ttgagaaata aacgactcgt taaagtttat caaacgatgt ccttactacc ttattttcta
      121 tcttgggtca ttatcagtta ctttgtttac gcttttttaa gtcctgacaa aggaatattc
      181 aatcagtgga tcactggaca tggaggagaa gctgtcaatt ggtataatga accgaaatat
      241 tggccgttta tcttggtgtt cattggtaca tggaaaggga ttggttataa cagcattatt
      301 tactttgcat ctgttatggg aatcgatccg acttattatg aagctgctat ggttgatgga
      361 gctagcaaat ggcagcagat caaacatgtg acgataccac aacttattcc actaatgacg
      421 attttaacga ttttagccgt aggaaacatt ttccgagctg attttggcct gttttataac
      481 attcctagga attccggtgc attatatgat gtgactcaag ttttggatac gtatatctac
      541 aatggcctga catccacagg agattttggt atgacggctg ctgcaggact ttaccaatca
      601 gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt tgatagcgat
      661 tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga gtccaagtcc
      721 gctctttcaa taagacggct aattttttct ctaatataat gattgcactc tttgctattt
      781 cctgtatttt gccatttata tttgttatcg ccatctcttt taccgatgag tccatattgt
      841 cgactcacgg gtacagtttt tggccagaaa agttcagtat atttggttat acgtacttgt
      901 tcgaccagat gcaggataaa attttccaag cgcttttcgt cactgtactt gttactgttt
      961 taggtacact gatcaatagc acggctactt ctttatatgc ttatgcgatt tcacggtcaa
     1021 actttccatt ccgtcgtttt tttaccgttt tttgtttaat tacgatgttg ttctcaccag
     1081 ggatggtggc caattatctt gttatgacaa atcttcttca gttaaaagat acgatttggg
     1141 cattgatctt gccaatggct gtcagtccct tcaatattat tgtgatgcgc acattcttta
     1201 aacgttcggt ctctgattcg attattgaat cggcacggat cgatggagct agcgaactgc
     1261 gaatatttgt tcagatcgtt ttaccgttag ctgttccagg tattgcgaca atcagtttat
     1321 ttgccgcatt gggttactgg aatgactggt tcaatgcttt actatacatc caagatgata
     1381 agctagttcc actacagtat cttttgatga aaatccaaag taatattcaa tatctgacac
     1441 aaaattcagg agcaggaagc caattgacag gcggattagc ctcagttcca ggagaatctg
     1501 ccagaatggc gatcgtagtc atctcgacgt tgcctattgc gatcagctat ccatttttcc
     1561 agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga aacaaaaata
     1621 taaatcataa aattaggagg aagaaaaatg aagttatgga agaaactagc ctttacaggt
     1681 gtttcagcgt tgttgttggg aacattagca gcatgcggag gttctggttc taaagaccag
     1741 gcagaagcta gcaattcgga cactttgcag atgtatcaaa tcggagataa accagataat
     1801 ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg tgtaaaagtc
     1861 aatatcaatt acattggctg gggcgattac gaaaagaaaa tgaacgtcat catttcttcc
     1921 ggggaaaatt atgatatcgc ctttgcgaac aactatgttt ccaatgccca aaaaggagcc
     1981 tttgctgatt tgactgaatt agcgcctaag tacgctgaaa aagcttataa tgatttagat
     2041 gaagcatata ttaaaggaaa tctggtcaat ggaaaattat atgctttccc agtcaatgga
     2101 aatgtctttg ctcaacaagt acttactttt aacaaagctt tactagataa atacgatctt
     2161 tcaattgatg gaatccaatc ttacgctgat gcagaaaaag tcttgaagga attccatgaa
     2221 aaagaaccaa acacggcagc ttttgccatt ggacaaggct tcaaagtcca agggaacttt
     2281 gattatcctt tagggaatac gttaccattt gctattgatc tggatggaga tgattcgaaa
     2341 atcatcaacc aatacgataa tgaaacgtat atcggactgt tacgtacaat gcatagctgg
     2401 tacaaacaag gactcattcc aagtgacgct gcaacgagca ataaggatta tccactagaa
     2461 ggaaatactt ggctaatgcg tggagaaaca caaggaccgt atgattatgg cgatacaatt
     2521 ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc actaaaatct
     2581 acagctcaag cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa taaagaaaaa
     2641 tcagtagaac ttctagggct gcttaacagt gatccagaat tattgaatgg gcttgtttgg
     2701 gggatcgagg gagaagcatg ggaaaaagtc gatggttcag atgataaaat caaattgcta
     2761 gatggttatc agccaaatac acatatgtct gcttggaata caggaaacaa caagattcta
     2821 tatacacaag agtctattac ggatgacatg attgccaaac gtgatcaatc gattgctgat
     2881 gcagaaactt caccgatttt aggattcagc ttcaacactg acagtgtcaa aacagaatta
     2941 agtaatattt ctaatgtcat gaatcaatat ttagatggat tgaatacagg gactgttgat
     3001 ccagatgaaa cattaccgaa attgaaagac gctttggaca aggctggcta tgataaggta
     3061 ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag aataataatg aataaagaca
     3121 aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg tgttcaaaag
     3181 ccagtttctc ggtattaagc taaatatatg taaaaatgtg gaaagccata ttcacaaaat
     3241 ttttcttact actcgaaact aagcggctat ttcacaaact cttggtaaac ggtgttcaat
     3301 cagctaaccc tcggtattcg ctcacaaaac gacaaaatat gaggaactat ttttccgttt
     3361 gtttctctaa taatcaggtc aaggtgctga ttgcccaacc tttttttgta taatgagaaa
     3421 gtataaactt aatgtttgga ggagaagata tgttagggaa ggttttggaa gagttattta
     3481 tccggatctg ggtcattata aaactaacct tgtatttctg gatatataca tttgccggcg
     3541 ggatcatctt tggattaggt gcggcatgga aaacagtcaa tgaactattt tatttgtacg
     3601 gattcgagta taaagaaatc acaataaaaa gaggctggaa tatatataaa agaaattttt
     3661 taagaggcaa tctgctattt agtttatttt tatctggtac tgctttattg agttacaatt
     3721 tatatttatc tgttcaaatc aaagggctca tttttttagc tattgatttt attttacttt
     3781 ttgccttgct ctgcctattt gctacttatc aatacagtat gattttagat agtgaatata
     3841 cgatctctgt tcctaatctg ctaaagctaa gttttatttc ggttttttcc agcttttcgt
     3901 cttttctaaa aataattatt ggtagcggta taattttagg cgtgacttgg caattcaaag
     3961 gattgatcct gtttggtgtc attggtcttt tgacggtttg gaatggtacg atgacaacac
     4021 attggcgtga agaattagat aaacagttgg aaagttatga ataagtttcg gattctattt
     4081 tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg cggttatcat gatcggtgta
     4141 acgatgactg ctaccgttat ttttagtatc catacttggg aacaaaatca aaaacaggca
     4201 gaaaacatga caagtgatgc cgttcaaagc acaagcagaa tgctgaatga taaaacaacc
     4261 ttgagtagga tcatcaaaaa ccagcttgta gggaattccg aaaagatcga aaatgtgacg
     4321 acctacttaa caaaacccat agatcagtat ctgatgtatg tatacgaaca gcaaaacagt
     4381 accgatgaat tagtttcttt cccaaatcaa atcaaagatc tctacgcaaa ttatgaagaa
     4441 ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt attatgaatc ttctagagaa
     4501 aacaaaggtg gggaaataaa aaaggggaca ccaaaattac tagacgcctt ttatatcaag
     4561 ttgcctatca cacaaggagg agctgagtca ggaagtattt ttattggttt tcaaaagaaa
     4621 gaactagata tgattttaga aaatctaacg tcattcaatg gactctcttt atacatgatt
     4681 tctggaacga caaatcgact atacacattc catgataata aaatagctaa agaaacgttt
     4741 gaaagacaag aaatgatcat cacagagagt ctgaagaaaa attcacaatt gccgattgac
     4801 gatctggaaa aaaacaattt tgttcaacac caagaactgt ctgatgatta tcgtatatta
     4861 gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc cccgctgatc
     4921 attggtatca ttattttaga caatatactg cttatctttc tttataaaac attcaaacga
     4981 tattcacagc aagtatctat cgtcatgcag gccatggatc aaacagcagc agggaattta
     5041 gagacgcgga tcgacataac aaacacggaa tatgaactga aggaactttc aatcggtatc
     5101 aacgaaatgt tggatagcat caatcagttt gtagaagaca tctacaaatt agaaatcaaa
     5161 cagcaagacg cacatatgcg ggcgcttcaa gcacagatca atccacactt tttatataat
     5221 acattagaat atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt agcagatgtt
     5281 gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc aagagaaaac gatcacttta
     5341 aaagaagaac tggacttttg cgaaaaatat gtctatttgt atcaaatgcg ttatcctaat
     5401 cgagtagcct atcattttat gatcgatcct gatttggaaa agatcgaagt acctaaattt
     5461 gtgatccagc cattagttga aaattatttt aaacacggta ttgattttac tcgattcgat
     5521 aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat catcaaagat
     5581 aatggaaaag ggatgactga aaaacggcta aaacagattg aagaaaaact gtctcatcct
     5641 aaagtggaac ttcacggttc tatcggtctg caaaacgtga atgaacgttt acgtgccagc
     5701 ttcggcagca gttattatat gtcgcttgag aataatgaga caggtggact gaccgtttct
     5761 attacattca aggagggatg agcgtgtatc aagtgttatt ggtagatgat gaatacatga
     5821 ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt aggctttcaa atagtagcaa
     5881 cagcagagaa tgccaaagaa ggactagctg tacttgaaca gcgacagatt gatttagtag
     5941 taacggatgt gacaatgcca gaaataaatg gactggaatt tattgaagcg gctcaaaaag
     6001 aacgtcataa ttttgaattt atgattttat caggatacca agaattcgat tatttgaaag
     6061 gaggtatgca attaggcgcc gtgaattatt tgatgaaacc cgtgaataag ttcgaattgg
     6121 ttgaaagcct caagaaaatc aaaacacggt tagatcagca aaatgaacaa aaaaatcaac
     6181 aagaaatcta tcaagaactg ttattcagtc aatggttgaa tgaagaattg gatgaaataa
     6241 gtgaagaaga aataatagga caaataagtg agaagcagca taggatagta ttgattcaac
     6301 tggcaagaac tcatgatact cttatcaata gatggttgca agaacggcaa caaatctttt
     6361 actatcaacg aaattacgga gaccgtgtat tgatcacgtt gttgttggag gcagatgaag
     6421 ttgatggctt ctgttgtttt gttcaagaaa actttcctga tcaggaatgg ctgatcagta
     6481 tcggggaaga gacagattct ttggacaaaa tccctgaaac attccaacaa gcgaaagaca
     6541 atctacagtt gcatcaattt tatggagata aagacgagca tgttttttac gcagtacaag
     6601 cagaaacaaa agatcaaacc atcgattttt caagtttcag acgcgtgctg caaaataaac
     6661 aattggaagt cgctcaaaaa atgatcactg atttttttga acagtttcag ttagcagcca
     6721 tgatgccgga agatatacga tattccagtt ttttactgtt tatggagatt caacgggaat
     6781 tgattgatct ggaagatgag gaatatttac aaggaatcga aaaaatccat caggcaaaaa
     6841 cggtgcaaga acttcagcag ctacttctgt cattcatcaa aaagcatcag agacagaaaa
     6901 agtacagtga caatgtggaa aaagtaatcg taatcctgca ccagcattat caagaaccac
     6961 ttacactcaa agaagtgtca gaaaacctgc atttaaatgt catgtatcta ggacagctgt
     7021 tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa ccatttacgt atggaaaaag
     7081 cgaaacaact gcttttgcac agtaatcaaa acatcaatga gatagcaagt gagattggct
     7141 acaacaacac cacgtatttc tctaaattat tcaagaaaat cgtcggcaga tcccctaaag
     7201 agtaccgaga aaacagtgga gaatcataaa aaaaaaagcc aatgtaatga aataacttat
     7261 aaaaaattgt ctagaaatct aattttgggc tgttcttatt acttgaggca aggcagcttt
     7321 ttcatgacct ctcgataaac gg
//
LOCUS       DAYYAG010000131         7275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000131 DAYYAG010000000
VERSION     DAYYAG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.134"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1502)
                     /locus_tag="ACHARQ_002477"
     CDS             complement(<1..1502)
                     /locus_tag="ACHARQ_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410866.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDY"
     gene            complement(1522..1725)
                     /locus_tag="ACHARQ_002478"
     CDS             complement(1522..1725)
                     /locus_tag="ACHARQ_002478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410867.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            complement(1726..2415)
                     /locus_tag="ACHARQ_002479"
     CDS             complement(1726..2415)
                     /locus_tag="ACHARQ_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFM8410868.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2670..2990)
                     /locus_tag="ACHARQ_002480"
     CDS             complement(2670..2990)
                     /locus_tag="ACHARQ_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410869.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3023..4141)
                     /locus_tag="ACHARQ_002481"
     CDS             complement(3023..4141)
                     /locus_tag="ACHARQ_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410870.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4170..4964)
                     /locus_tag="ACHARQ_002482"
     CDS             complement(4170..4964)
                     /locus_tag="ACHARQ_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410871.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(5017..5370)
                     /locus_tag="ACHARQ_002483"
     CDS             complement(5017..5370)
                     /locus_tag="ACHARQ_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410872.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            complement(5472..5729)
                     /locus_tag="ACHARQ_002484"
     CDS             complement(5472..5729)
                     /locus_tag="ACHARQ_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410873.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            complement(5732..5956)
                     /locus_tag="ACHARQ_002485"
     CDS             complement(5732..5956)
                     /locus_tag="ACHARQ_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410874.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(6129..7172)
                     /locus_tag="ACHARQ_002486"
     CDS             complement(6129..7172)
                     /locus_tag="ACHARQ_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8410875.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2020 a         1523 c          909 g         2823 t
ORIGIN      
        1 aagtaatctg tatatagaaa aatcgcatca cacgtattca aatttttaag tatctttcca
       61 ttattttcgc ttacatcaaa cattttaata tttggtaata gttcagttaa tttattttga
      121 aaatttacat gtcctcccac taaaagaatg tttttatgtt ttagataagc tatcatttca
      181 gacagtgaag gctcttcagt tttaccattt aatgattgtc tatgttcttg ctcctgcaat
      241 tgctttaata ctttattttc aaacctcagc tgttcaagtt cttttctgat tccttttaat
      301 tctttaatct cttgcttcaa aagttcctct tctttttttg ccgatttgac taaagattct
      361 aaccgaccgt tttctttact cgcaatactt aattcttttc ttaatttgat tacttcagat
      421 ttgttttctt ttctaatact tgcaatttgt tttgctattt cataatcaga attcaggatc
      481 aattcttcct tagcattttt aaacaaccga gttaaaacga actgatataa caaactataa
      541 aaaactagca aatagtcaat ttgtcggtac tcggtattct gatcaattgc atatagagag
      601 attaaaacga aatacacaat atcattcagc tcgctaccct tgatctcaaa attattgact
      661 aagtaactat caatacccat caaatctgca aaatctccat acaaagaaaa atatgatttt
      721 gacaatatat ctatatctga atcgtcaaac ccatttacta ttttcccatc attgttagta
      781 gttgctaacg aagcaatagt gacttttgaa ttgggtttta caaatcctat acctaatagt
      841 tctttcatct ttttaatttc aagtttgtta tccttttgtt cggctttatg ctgtttcata
      901 ttgtaaagcg gataaaatgc tgttccgtaa gcacctttta gtacgttatc tataaaataa
      961 tcttcagaac ctgttttgaa tagaaagtta atcgcagcca aattagataa cagctcttca
     1021 gtgttgtgca tcaatttttt atcatttaat aaattatccc caagatattt cattgcataa
     1081 taagggtttg tttcgctact tatttttaat attttttgat agctattcct cacgatatta
     1141 accaaaatat ctgtctgatt aattgaatta cagtacaaag ccactcctaa tgccatataa
     1201 acattcaaat catgttttgc cgtaccgttg aataaaacgt cttcaacttt tgaattatct
     1261 cgtctatact ttttgatcat atttttgata ttttgatcat tctcttggta tatttttttc
     1321 actcgttcca atgccttggg tttgttggaa acagcaacag cgaagtctag ttctttgaaa
     1381 tcaatattta gtatctgtac ctcatcacct agattataat ctctatttac gctcggaaaa
     1441 ctgctgaaat ctaactgttt tttacctatt tttggcgaaa atattccttc gatttctggc
     1501 aaatgtaatc acactctctc cttatttatc tttagaaaat ttgtcactat tctccttata
     1561 tttatcaata aaccttttta atcgagcaat ataagcgtta acccttttag gttcttcttt
     1621 tcgccattca tcgtttacgt gaccattttc aaagaaagaa gctaaaagag tttccacttc
     1681 attaaccata tctgcgatcg tatatttttc tgtaccctca ctcaattatt catgaactgt
     1741 taagtgttca tagctaagtt cattcataat tttgttatag cgttttcttg ctttttcaaa
     1801 ttctttctgt tcgtcaaatt ccacttgatt cttcatgttg ttcttcctct ctgtttgata
     1861 ttttttcact tcttcaagtt tagcatctag ttccgcttta acttctgtca cttctttaag
     1921 cgcttgggat aacttatgag acaatttcgc ataagtttct ctcttgtatt catttcctac
     1981 tcccaactct tcggagtaga actcttctag tagtcttgca aaattaacat ttctcctgcc
     2041 agcatagttt tttagcttct caaaatcaga ttgaaagaca gcaacacttt tgtaatcctt
     2101 ttcaccctca aaatcaaaag catagtcata ctttcctgaa atcaattcat caataactgt
     2161 agcaatatat tgagagatag aaatattatc ttcacttcta tgtcgctcta taagattata
     2221 tatatcaggg ctgacatgaa acgtacttct cttttttgtt tgcctttttc taactgttac
     2281 gaccactttt ggaacttcac tatctccatt gatctctttg tatttttcaa taaataattt
     2341 tctagcttgt tctcgacttt ttgccggaat ttcatctaca attttatctt ctaaataaat
     2401 atagtgtgtt ctcatacatt cgtcctctat cttctttact ctcaatcttc atgatcttag
     2461 ttcttacctt gtttactctt aaacctttca aaaaaatcta gtcaaaatca aacttgaaat
     2521 catcgtctat cacaacaccc ttaacttcat ctttagattt cccaaaggaa cccttatttt
     2581 caaatccttt atttttgaat gatgatggtt atggaaaact tcctttgaaa ttctgacaag
     2641 cataactagt cagaactgtt ttacttagat taagctttca aaagatactg atgaagaact
     2701 aagttctcag taccttccca ttcttcaata aaaaatattt ctccttcttt tacccactct
     2761 atagctaatt tcttataatc aaataaacca tatgtgttta agcctttgtc tttcattaaa
     2821 ctcaaaaact gatccccttt taatctttct gtatcatcca tcagaaattc aactaaatct
     2881 ttatccatgc gtgcaacttt ttcgtctttt agtgccattg atttagcata gtcagttgaa
     2941 tcttttagaa taataacacc tacatttcct tccacaattt tcttcttcat atttacctcc
     3001 taccatcttt tccaaagagt atttagtcaa aatcaaactc gaaatcatcg tctatcacaa
     3061 cacccttaac ttcatcttta gatttcccaa aggagccctt attttcaaat cctttgtttt
     3121 taaattctaa agctttttcg acttcttctt tggatattgt atgaactttt ttcttaacta
     3181 ctccaagttt atacagctct tcaaactctg gtacttcgga tctgaaatac aatccatcag
     3241 tccgaatttt taatagccca tatgcgctat gaacgtattt ctgttcatat ctaaccatac
     3301 gatctctaaa tacgcctgct accatggagg aatacgctcg attatacata ctgtatttat
     3361 cgaaggtgct tgtttctcct tctcttaatg atttttgtcc gatagcacca aatactttcg
     3421 ttctaagatt tttataaagc ttcaacgtat caggatcagt ttttaatggc tcgattcttt
     3481 caaaatattc tttgagtaat ccttttttcg tcaaaaatgt gtggctatct aggacttcca
     3541 tctctttaat atcataataa tcaaggataa gtgttagctc aatctccgat aaccaaatag
     3601 gctcattagc tgttttggga ttttcgccat aatccgcaaa catcgactgt gattttggat
     3661 tagcatatgt tccttctttg gctgttcctt caaaattaac caaaaagaac atatacgttc
     3721 ttaaattttc atacggtctt acttttggtc tatttatcgc tctcaaaata gcttgatctg
     3781 tattatattt tgctttcaaa taagtgtttg acggcaaagg ataatttctc atgatcctcg
     3841 aataggcttc gttgaaatca tattcgatca tctttgctcc ttcctcaaac aaatccaagt
     3901 tatacatgct accggaggta ctggctctat aggctgaact tggaacaaag aaaggtgcat
     3961 cttccggctt atcatagccc aaaaaatgta ttcctgtttg cttgttctga cggtaaatgt
     4021 ccatcaaaag atatttgatc gtactaaaag aatctaattt tcctcttgtc atccacccta
     4081 atccgacaag tcttttaatt ttattctgca tttcaggtgt gtatatgcta ttgtttccca
     4141 tttatctgct catctccggc ttttttattc tagaccctat atttgttcat tacaccactt
     4201 atctcgtcta caaaggctcc cagatcgtgt gtaaaggcat cgtagtaatc ttcaatctca
     4261 ctcgattcag ttctccggtt tcttccgttt ttatttctga atagatacat ctgcgtttct
     4321 cttaaatccc ttgctttttt cgcaagtgct aataaatccg taaaattatc aataaccata
     4381 atgtcattga tcataaaacc ttcttcatga ccatactttt cagccatctc gataaaatca
     4441 ttaacatcga acaactccaa taattctgct ttatatttct ctaacaatgt atttaaatag
     4501 cgatttctca attcccatga actttcacca gttccgtatt caaccttgat ccctaaattc
     4561 ttagtgtagc cttttgctgt gtatcgtcca atgaagtctc ccttattccg attgctatct
     4621 tcttccttga aaaactcacg ccaaattccc tttttctttt taggaatcag acctctgctc
     4681 ttgaaatcat tatatttttt tgaagcttcc cttcgtgtat ctaagtacct ttgcttcagc
     4741 tctgtagctg attcattcgt tccatattca acagtgatcc ttaatcgttt gctatagcct
     4801 tttgctgtat atcgtccaac aaagtcaccg ccacttcgat cactactgac ttccttaaca
     4861 aattctctcc atataccttt tttgtctttc ggtattaatc ctcgtctttt catatcttga
     4921 tatttttcag aagcttcttt tagagtattc ttttttgcta ccatgtcatc acctcgcaat
     4981 ttagttaact ttatacacgt ctacaaaaaa atatgattac ttacttttta aattgaaaaa
     5041 ctcgttgaaa tgctcttctt tcaacacaac aaaagcatga taacttaacc ttaaaatata
     5101 atctccatat tcaactggat aatctttggt tataactcta attaccgctc ttccgctatt
     5161 tacaagttca ctttttttct tcccccttcg tactcttcgt atactatgag gttttacaaa
     5221 ttcgactact tgcttccaat tatcattttt atattgaata cacagtggtg attttttcat
     5281 ttctatatac atgttcttat aatcttctac tttcttattt tctttaacta gatgtttatt
     5341 attctcataa gacaaatttt cgcttatcat ttaatatttc ccttcctttt tttgcattat
     5401 atttagcaaa cattcaccat ttgaatttga catagcttta agacagatat tttatctttt
     5461 caaacattaa gttatttgtc tttttcagaa gtggtatagg atcgaagtcc ttcacgtttc
     5521 attatgtctt ctgcttttcc aatgctgatc cctttccaat cacgccatcc tacgcttccc
     5581 ttattttgac gataggcttt aatagctgat tcggatattt gccatcttgt agccagctct
     5641 tttgcggtgt acttatttga gaaaagtaac ttctctatca gtgaaatatc aatcatttta
     5701 ttctcactcc tgaaatcttc attaatcatc tctagctagc tatatcttca atcaattcta
     5761 atatgtcgga taaaccatca acagcttctt caagttgatc aacggattct tctgcttttt
     5821 catatctttc tgatccctgt agatttttag gtatattttc tttgtactct tcttcttgaa
     5881 ttacttctaa ctcatccttt agctcggaaa gtcgctcgct tatttcttct aacctttttc
     5941 ttcttctttg gttcatcaaa atcacctctt cgcataatct ctacgatttt aatataccac
     6001 ttttgtgtac tacattcaat attttttgtt atagattcgt attacaaaaa tttcctctaa
     6061 aaaaataaga gcctatttac catttcagta attaagctct tatttcattt agttgataat
     6121 tctatcaatt aacgtacagc aaccgccatt ccagtcacat taaatgctct ctcatacgtg
     6181 cttgcattga tccattttgc gccaaataca gggtcaacaa catggtatgc accatttctg
     6241 aagccatcca cgagaactac atgtccattc catactgcac gaccccaagg catttgtttg
     6301 aactctggcg tagcatagtt ataagtaatc caagttacaa ccggattacc attgcgaatt
     6361 tcattcttga ttccattcgt tcctgaacca gtgatattga cggcattacc gccaacgttt
     6421 tttgcccatt taactactgg gtcagccatc atgccttgat acgttccatt aacgttatga
     6481 cgccactctc ctgagtaacc atgataagga ttattgtcat tagcttttgg catatagttt
     6541 acaaaactgg ttaagctttg atttgtggct acacctttgt agtgtaaacc ttctaataaa
     6601 gaagctcctt cgcataacat tgtatttcct tgccatacaa caggaacgtt caacactttt
     6661 tcagtgcttg gagcaggtgt aggatttggt acgctgtttg gcattgcata ccagcctgta
     6721 ccttcaatct tccaaccttg tttactaatt acgttctttt cattttgatc taacgtgtag
     6781 tgatgccaat tcacgtattt gttatataaa cgtgttacag gaactctatt tgctccgttg
     6841 taggaataga acgcaacacc ttcgtacgtc catcctgtgt gtgaaatctt atctctttct
     6901 ccggcatcaa ctgtgtaaaa atgttcaccg ttataccgat tatacaaacg ataaacagga
     6961 gtaccacttg ttggagcttg ccatgcgaca ccttcgtaat gccatccata acgtttcaga
     7021 ttgtctcttt ctccaacgct tctagtgtat agatgctctc cgctattggg attatacata
     7081 cgatacatgg aaatagagct ttctgctgaa actgaaggtg ataataatac tagccccatt
     7141 gtgaatagaa ttcctactaa aaattttttc atttgatttt accccttttc attttttatt
     7201 gtcattattg catgaaaatt attcgtaaga ttgccaatta cataaacgat tgttttaaga
     7261 tcataatcgt tgttg
//
LOCUS       DAYYAG010000132         7151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000132 DAYYAG010000000
VERSION     DAYYAG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.135"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..861)
                     /locus_tag="ACHARQ_002487"
     CDS             complement(274..861)
                     /locus_tag="ACHARQ_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410876.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1036..2358
                     /locus_tag="ACHARQ_002488"
     CDS             1036..2358
                     /locus_tag="ACHARQ_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8410877.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2513..3289
                     /locus_tag="ACHARQ_002489"
     CDS             2513..3289
                     /locus_tag="ACHARQ_002489"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8410878.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3282..3851
                     /gene="rnmV"
                     /locus_tag="ACHARQ_002490"
     CDS             3282..3851
                     /gene="rnmV"
                     /locus_tag="ACHARQ_002490"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8410879.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3844..4728
                     /gene="rsmA"
                     /locus_tag="ACHARQ_002491"
     CDS             3844..4728
                     /gene="rsmA"
                     /locus_tag="ACHARQ_002491"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8410880.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5179..5808
                     /locus_tag="ACHARQ_002492"
     CDS             5179..5808
                     /locus_tag="ACHARQ_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410881.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5932..7041)
                     /locus_tag="ACHARQ_002493"
     CDS             complement(5932..7041)
                     /locus_tag="ACHARQ_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410882.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         2261 a         1266 c         1463 g         2161 t
ORIGIN      
        1 cagaaaaaaa gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct
       61 tttttattgt ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta
      121 tatctaacag tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt
      181 tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc
      241 gatgattgtt ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat
      301 ttttgtcttc aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac
      361 ataccagaaa atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg
      421 taacaagatc aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat
      481 gacttcacta agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt
      541 catccgccag ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa
      601 aataccgata ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat
      661 cacaggacct ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg
      721 tgataacgtt gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga
      781 ctgctgttgt tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg
      841 ataagaacgc ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata
      901 cgaaaaataa aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa
      961 aaaagaatgg taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga
     1021 agaaagagga tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta
     1081 gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact
     1141 gctagtactc agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg
     1201 atctgtgctg gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct
     1261 gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa
     1321 agcttgattt actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg
     1381 acgaacttat tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt
     1441 tctgtgacag ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtaacg
     1501 ttgattgtta aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga
     1561 caaggaactg tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga
     1621 ttgagcagtg cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca
     1681 atggcagggg aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg
     1741 agttttgtaa cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt
     1801 gatcatcttg ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga
     1861 atcggtggaa agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt
     1921 tatacactaa ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc
     1981 agtaaacagc tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc
     2041 cagttagcag tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg
     2101 ttgatttttg tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga
     2161 aaaaaagcgc cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt
     2221 attgcgattc tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta
     2281 gcattgatcg gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa
     2341 aaacgctcag aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc
     2401 tagatcttcg tgctttttct atttcaataa aacaagttct tctattttga tttttgctac
     2461 ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt
     2521 tgattctcat acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca
     2581 gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat
     2641 tgaaaagtct ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca
     2701 tccgacagag gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac
     2761 tttaccaaag gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc
     2821 gaaagaagtc caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct
     2881 accaatcagt attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga
     2941 agatgttcga gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa
     3001 acgatttctt gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc
     3061 attagacgtc caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga
     3121 tgccccttat ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg
     3181 ttatacagta gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca
     3241 aacatggaca aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc
     3301 aagagattat agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg
     3361 cagatacgat agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac
     3421 atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa
     3481 taagaaaaat cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac
     3541 aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt
     3601 tagaagcatt gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca
     3661 ctagacaaga ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag
     3721 aaaaactagg tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt
     3781 tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac
     3841 aacatgagtg aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa
     3901 catggttttt catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta
     3961 aggaaaatcg tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca
     4021 ggaatcggtg cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag
     4081 atcgatgatc gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact
     4141 attgtacatc aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat
     4201 gaggagcttc cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg
     4261 atgcattttc ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa
     4321 gtagctgatc gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct
     4381 gttcaatatt atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct
     4441 cagccaaatg tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa
     4501 gtgacagacg aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa
     4561 acgctttgga ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta
     4621 gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta
     4681 caagaattcg ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg
     4741 tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
     4801 gttcctcatg tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg
     4861 tttaagaaag aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa
     4921 aacagcttct catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac
     4981 tattatgccg aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt
     5041 gatcaaaaaa ttcttatact tgatactcac actcattttg tatggattta gaaaagaata
     5101 tgtgtcttga cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca
     5161 tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta
     5221 ttgattggga tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct
     5281 tcctttactt tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt
     5341 gcagtggctg ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc
     5401 gcattgcgag cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag
     5461 catccgagta tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg
     5521 ttcaattttg tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt
     5581 tactttattg gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc
     5641 atcttgatgg ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt
     5701 atagcaggaa cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa
     5761 aatcggaaag agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc
     5821 aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc
     5881 ctaaaaagag ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag
     5941 tttgatgact tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag
     6001 gtcgactatt tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt
     6061 agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc
     6121 tgatttcact acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc
     6181 tggacgaata ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt
     6241 ttttccttca ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa
     6301 tgtcacatta aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac
     6361 gggtgtatca taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat
     6421 aacgattcgg tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt
     6481 tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc
     6541 atctaaattt gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg
     6601 tcccaatgcc acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta
     6661 ttctgtcaga cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc
     6721 atatttacgt aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa
     6781 tgcgtagttt tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac
     6841 ttttccgccg atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa
     6901 agtagttgac tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat
     6961 tgatagatta aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt
     7021 tttcagagcc atttctacca taatacgttc cacactttct tttttagttt tattaactat
     7081 ttaaagtata aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct
     7141 tttttttgtc a
//
LOCUS       DAYYAG010000133         7047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000133 DAYYAG010000000
VERSION     DAYYAG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.136"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..216)
                     /locus_tag="ACHARQ_002494"
     CDS             complement(<1..216)
                     /locus_tag="ACHARQ_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410883.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPN"
     gene            354..1868
                     /locus_tag="ACHARQ_002495"
     CDS             354..1868
                     /locus_tag="ACHARQ_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8410884.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2271..3254)
                     /locus_tag="ACHARQ_002496"
     CDS             complement(2271..3254)
                     /locus_tag="ACHARQ_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8410885.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3259..3906)
                     /locus_tag="ACHARQ_002497"
     CDS             complement(3259..3906)
                     /locus_tag="ACHARQ_002497"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8410886.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(3991..4623)
                     /locus_tag="ACHARQ_002498"
     CDS             complement(3991..4623)
                     /locus_tag="ACHARQ_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8410887.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(4639..5190)
                     /gene="coaC"
                     /locus_tag="ACHARQ_002499"
     CDS             complement(4639..5190)
                     /gene="coaC"
                     /locus_tag="ACHARQ_002499"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8410888.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5187..5924)
                     /locus_tag="ACHARQ_002500"
     CDS             complement(5187..5924)
                     /locus_tag="ACHARQ_002500"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8410889.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(5921..6910)
                     /locus_tag="ACHARQ_002501"
     CDS             complement(5921..6910)
                     /locus_tag="ACHARQ_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8410890.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         2156 a         1356 c         1453 g         2082 t
ORIGIN      
        1 atttggaatc tgtatcttta atatcttttc cagaaaataa gggatttcaa ataaaattaa
       61 tgtcagtaat aaaagcattg tatttcccca gtgttggttc cagacctgat ccactagcat
      121 aataacgacg atgatacgta ataaaatata gattccgttt gtgattctac ctgcagtttt
      181 tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa taactccttc atgtatatcc
      241 ttaattatac taagaagcgt gtgtttacca aagctatatg acttaaagaa aaacagagtt
      301 gatattgtga aaaaaatcac ttaatatagt ataaggagaa aagggggata caaatgaaaa
      361 aattagttgc tggtttatta acagccgttg cacttcttgc tatgagcgca tgcggcgcag
      421 atcagaaaaa tacagcgcag acaagttcta gtacgaagga atctgccgaa gtatcatcag
      481 gcgcttctga acaagtatat acagatccaa aagaaatgaa agaatcttat gatgtcgtga
      541 ttattggagc tggtggtgca gggatggcag cagctatcga agcaaaagat gctgggttga
      601 atccagtaat tttggaaaag atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg
      661 gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg
      721 ccttctatga agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat
      781 actttgtcga tcactctgct gatgcggtcg attggctaga tcaaaacggt atcaaactgg
      841 acaacttaac gatcactgga gggatgagtg aaaagaggac acatcgtccg tctgatggct
      901 cggctattgg tggatactta gtggaggggt tattgaataa cgtccatgag cgggaaatcc
      961 ctatttttgt caacgcagat gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag
     1021 tgactgtaca tgttcaagga gaagaaccaa aagaggtcac cggaaaagca atcgtagtta
     1081 ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc gatttagctg
     1141 attatattac gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg attgaaaaat
     1201 taggtggaca aaccgttgat atggataaaa tacaaattca tccaactgtg cagcaggatg
     1261 agggggtctt gattggtgag gttgttcgcg gagaaggagc aatcttggtc gatcaagagg
     1321 ggaaaagatt tgtcaatgaa atggatacaa gggataaagt atctgcagca attacagcat
     1381 tgcctgaaaa atcagcttat ttgatttttg accaaggcgt gcgtgatcgt gccacagcta
     1441 ttgaatttta tgaccaaaaa ggatatgtca caactggaaa gacaatggaa gaattagcag
     1501 aaaaaatcga tttaccaaaa gaaacactag caaaaacagt tgatggatgg aatacggatg
     1561 ttgcggagaa aaaagatgct caattcaacc gtgccacagc catggaacat gacttatcgt
     1621 ctccgaatta ttatgcgatc aaaatcgcac caggaatcca ttacacgatg ggcggtgtga
     1681 aaatcaatac gaacactgaa gtattagata aagaaaacca gccaattagg gggttatatg
     1741 ctgctggtga agtcacaggt ggcttacatg gaaacaaccg gatcggtgga aattcagttg
     1801 cggagatcat tatttttggg cgacaagcgg gcatacaagc agctcgattt gcttcggaaa
     1861 atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg agctaatacc
     1921 gagggtcagc tgattgaaca ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca
     1981 agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt tggacttatt
     2041 accgcagatg caagcttttg aacaccgttt attcggaaaa agagcgtggg acaaaagtaa
     2101 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gaaattcctt
     2161 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
     2221 tcttggagtt aaacgcttct gtcccagcct cttttttgat cagcatgatt ttattcagtt
     2281 atcttcaaaa tgatttttcc gatgtttttg tttgcttcca tatattcatg cgcttgtttg
     2341 actgcgctga attggaatgt acggtcaatg attggccgaa tcttgccatt ttctagaaaa
     2401 ggcccagctt tttgcataaa agtttgtgcg agctctgctt tatactgatc acttctaggt
     2461 gtcaataatg taccaagcag ttggacacgt ttcccaatca atacaccaag gtctatctca
     2521 ggcacgatgg tcccacctag cataccaatc aagacccaac gtccatcaac ggctatagaa
     2581 gctaaattct tttcccagta agaagcgccg atgaaatcca aaataacatc gactccccga
     2641 ccttcagtga tttttcgtac ctcttcttga aagtcttgtt ttttatagtt gatcaattca
     2701 tctgcaccaa gttctttaca aaaggcgagt ttttcttctg atcctgcagt aacgatgatt
     2761 ttcgcgtgag aaagctgtct agctaattgg atcgctgctg ttcccacacc acttgctcct
     2821 gcatggatca agacagtttc gccttcttgt aattttccaa gccagtaaag tgtttgataa
     2881 gcagtcaaaa aaacttctga gattccagca gcagaaacat agtctagcgt atcagggaga
     2941 aggattgcac gatcggcagg catcacagca tattcggcat aaccacctag attgactaat
     3001 ccgtatactt ttgtcccagg agtaaaagtg gagatatcac ttttattctc aactacctct
     3061 ccagctactt cgactcccaa aatagggtag ggaggtttca aagagactga ttctcttttc
     3121 ataatatctg ttcgattgac agctgctgta tggactttga tcagtaattc gccttgtttt
     3181 ggtttaggaa caggcacttc aatttgttct aatacatcta cagaaccagg atgcttgatt
     3241 gaaatggcac gcatggatct attcctcctt tgcccattct tggaatgctg tattcaactc
     3301 tttgctacct tcagaaagta ctagagtcgt acgagaaata tccgtttctt ccactagagc
     3361 ggcaagtaca gcagcaactg tagagcgagt gatcgttttt gatgccttct cattaggctt
     3421 agttaattga atggaagtaa cttcttcatc gttagtcaat gtgactggtt gaagaatgac
     3481 aaaatctaag ttagttcgtt tcagccattc atctgcataa tgtttagtaa tataataatc
     3541 tgtcatggac tcaggccatt ttgctggttc atctgcataa acagcactga ccatcagata
     3601 gcgagagaca tgcgcatttt cagctgcgat cactgttttg attgcgccat ccaagtctac
     3661 ttgcagaaga ttctttcctt gagagccggc agcaaaaatc aatacatcga tgcctttgaa
     3721 tgcttcggtc attttttcca cggaccaagt taaatcaaaa gagacatacg aaacattctc
     3781 tttttttact aatgtttggg aagcaacatc tctcacccct gcaaaaattt catgggaact
     3841 tggagctaaa tcttttatca ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat
     3901 tttcatttgg atcatccttt cgacgtcaaa tctttccatt caatagtaac accaaaagag
     3961 aaaaaaatca gagatcggct acgatgacta tcagttagta agagaagggt gcttgaagta
     4021 tcttaacaag acattgcata ctgctgcagt cgcaagacct gcgacgattc cttcaggtat
     4081 accacttgta aaaataaccg ctagaatagc tgaataaaca gcatttaaat ttgttccgat
     4141 gactttggca taatcttgct ggaataaaaa gtagatcata ttcatcacta aaatcgtatt
     4201 ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg gaatctgctt tttgtttcat
     4261 tagtttgcgc agacctttat aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat
     4321 aaacgcgatc agcaaggctt tccagcttcc atgttctgtt ccgatgactg ggataaacgg
     4381 agaaaagaca aaagacaaag gtgtttggat aactgtgcta cggatcatgc tgatcagtcc
     4441 aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa acgatcgaag caatgattac
     4501 aggaatatgc atggttgttg cattgattgg accgataggg ataaaaccta aaggcgtaac
     4561 agctaataaa atcaaaatag ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt
     4621 cataagtact ccttttttct aagcatttct ttcattaaga gtcagcatga cctgttcgat
     4681 gatcgtctga atatcagcta aggcaccttt tccaaaatct ccacaagcta aaagagattc
     4741 tcttggttca atttcctgat agccgacaga tctcaatgtt tccagattac gctgagtgat
     4801 cgggttttga tacatattcg tgttcatggc aggggcaatc aattttggtg ttcccactgg
     4861 aagcgccatt gcgactgttg aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat
     4921 aatgtttgct gtagcaggcg cgatgagaaa caagtcagct tgttttgcta gttcgatatg
     4981 attgattacc tgcggatttg attcttgcat gacgtctgta tggactgcgc gcttcgacag
     5041 agattgcaag gttaatggcg tgataaaatg tgtgctgctt ttagacataa tcacatctac
     5101 ctgggcacct aatttgacga attgacttgt aagatcagcg cttttgtaag cagaaatact
     5161 tccagtaacg cctagtaaga tctttttcat tttgattcct ccagtctaat tttctcagta
     5221 atcaatgcag caatctcaga tttactttga gcttcttcca ctgttccatc tttagataag
     5281 agatagccgt ggtgtgtctc gccatggatt tccgtcaaat catttgcgaa aataaaatca
     5341 gttttgttat ttatcagact attttttgcg actgccagaa gagattcctt tggtacatct
     5401 actaatagtt tgaaaccaat gatttttgct tttggctgcc attcacgtag ataagaaatg
     5461 atttttggtg ttttcttcaa aacgagcaca agatgctcgg tgttagaaga gatctttgaa
     5521 acagtggtat ctgtctgttc attttccgca aaccaattgt ccagttcttc ttgcgttgaa
     5581 gaagtgggta ggttttttgc cagctgttct tctgaaaaac taaaagcagg agtaaaatca
     5641 ctgactgcca tactgtggat aatcgcatcg tagtgttctt ttttcgttag ttcttctaat
     5701 tgtaaaaata agtccagtgc agattcgatc gtataaatcg ttatattgga gtgtcttttc
     5761 ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa
     5821 taatcagcta atgcttgccc taatcgtcct gtcgaatgat ttgtaatagc acgtacttga
     5881 tcgatttttt cagtcgttcc tccagctgtg attagaatct tcatttatgg tcacctacat
     5941 ttactagtgt tcttccttgg tgtgttccag caagtagttt agatgctgtt tcaggcaatt
     6001 gatccagagt aatctcttgg acgaccagtt catctgcaat ctgcaaatcc gccatctgtt
     6061 gccataaaga aagacgttga tctattggta cattgacaga atcaattcca atcaactgga
     6121 tgtttcgcag aataaaaggc aagacagtcg cgtttagttt gattccccca gcattgccac
     6181 ataaagctac tgcaccgtta taagagatca atggaagaag actgcttagc tgctcgccac
     6241 ctactgtgtc gatgacatag tcgatttgct gttttcctaa agcttttgtt gtctctggca
     6301 gaaattcatc aggtcgtacg acttgcaaag cacctagact ttttagccat gtgacagact
     6361 cttcttttct ggaaaaggca atgattgact ggaatcctag tttatgtaac agggctatcg
     6421 ctgtactgcc tacaccgcca gaagcaccag taacgacaac ttttttatcg gaggtcatac
     6481 cttgacggat taaagcgttc acacacaaca tagcagtaaa ccctgctgta ccaaggatca
     6541 tcgcttgacg cagcgtcata ttttttggta gaggaactaa ccagtctcct ggtacttttt
     6601 gatattggct ataaccgcct ggatgggtga cgccaagccc aaaaccagta gcaagtacaa
     6661 gatcttcttt tgaaaaacgt ggattgttac tttggacgac agttccggat aaatcaatcc
     6721 ctggagtcat cggatatgtt cgtatcacct gaccagactc gctcattgcc aatgcatctt
     6781 tataattgac atcagaatag gcaattttta ccaagacttc attttcagcc aactcagtaa
     6841 atggcagatc actgattgct gtatggaaaa caggttcctt tattacctga aaagatcgaa
     6901 aagcgttcat tcgtgagaca ctccttttaa aattttcttt tatcatacca taaagcctac
     6961 tcttttattc ttgttcttcc gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa
     7021 acaactagaa tttattccaa aagataa
//
LOCUS       DAYYAG010000134         7005 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000134 DAYYAG010000000
VERSION     DAYYAG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.137"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..1131
                     /locus_tag="ACHARQ_002502"
     CDS             190..1131
                     /locus_tag="ACHARQ_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8410891.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            1133..2023
                     /locus_tag="ACHARQ_002503"
     CDS             1133..2023
                     /locus_tag="ACHARQ_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8410892.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            2066..2866
                     /locus_tag="ACHARQ_002504"
     CDS             2066..2866
                     /locus_tag="ACHARQ_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8410893.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            2881..3732
                     /locus_tag="ACHARQ_002505"
     CDS             2881..3732
                     /locus_tag="ACHARQ_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8410894.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            3933..4394
                     /locus_tag="ACHARQ_002506"
     CDS             3933..4394
                     /locus_tag="ACHARQ_002506"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8410895.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            4420..4872
                     /locus_tag="ACHARQ_002507"
     CDS             4420..4872
                     /locus_tag="ACHARQ_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410896.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            4978..5172
                     /locus_tag="ACHARQ_002508"
     CDS             4978..5172
                     /locus_tag="ACHARQ_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410897.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            5203..5589
                     /locus_tag="ACHARQ_002509"
     CDS             5203..5589
                     /locus_tag="ACHARQ_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410898.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            5586..5789
                     /locus_tag="ACHARQ_002510"
     CDS             5586..5789
                     /locus_tag="ACHARQ_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8410899.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            5786..6082
                     /locus_tag="ACHARQ_002511"
     CDS             5786..6082
                     /locus_tag="ACHARQ_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410900.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            6158..6574
                     /locus_tag="ACHARQ_002512"
     CDS             6158..6574
                     /locus_tag="ACHARQ_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8410901.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            6835..6972
                     /locus_tag="ACHARQ_002513"
     CDS             6835..6972
                     /locus_tag="ACHARQ_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410902.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
BASE COUNT         2535 a         1092 c         1581 g         1797 t
ORIGIN      
        1 gttagtgatt gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa ttaccatgct
       61 tgtgctttgc gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg
      121 acaactattt aactgatcct gattatgcta aaccaccata tgaagcacca attgatgagg
      181 aggatgaaga tgagtaaatc taccttagaa atgagccatc aagaatggct tgaagaccgt
      241 aagaaaggca tcggaggttc ggatgttgga acaattttag gattgaataa atggaaatct
      301 ccttatcaac tatggctaga aaaaacagga caagtagtac ttgaagaatc aggaagtgag
      361 cccgcttatt ggggtaatgt tttagaagaa gtggttgcta aagagtttca ggaacgtaca
      421 ggcaaaaaag tacgcagaag aaaccaagta tttgaacatc cattacatcc atttttaaga
      481 gcaaatattg atcgggaagt agtgggagaa aatgccattc tggaatgcaa aacagctaat
      541 caatttctcg gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt
      601 caacattaca tgaacgttct aaacaaagac tattgttaca tcgctgtctt aatcggtggt
      661 caaaaattta tctggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa
      721 caattagtag agttttggga aacaaacgtt cttggaggta tcgagccaat tattgatggt
      781 agtcaagcga cagctgattt cttgaaagag aagtatgcag atgttgaaga cgttcaaacg
      841 acgttaccaa ttcatttcga tgaactagtt gaacagaaaa acgaactcaa acggactaag
      901 aaagaaattg aatcagctat ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa
      961 cgtgaagcca gtatcggtat cactcaaaaa aacatcatca gttggaaatt tgtccgtacg
     1021 agacgtatga actcgaagaa actagcagag aaatatccag atgtcgcaaa tgatgaagag
     1081 atttataacg ttactgaatc aagaaggcta accgaaaggg agatcaaata atatggcaac
     1141 aaatgaatcg ttaaaaaatc aattggcagc aaagccacag aaacaagttg caccaggtca
     1201 attaggactt aaagctttga tgaatacacc aacaatgaga aagaagttcg aagaggtgct
     1261 tcatgacaat gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta
     1321 tcttgcagat agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa
     1381 tcttgggcta gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa
     1441 gcaaacagga aaatgggaaa agaaagctca attcatgctt ggctataaag gatatatcca
     1501 attagcccaa cgatcaggta aatacaaagc attaaatgtg atcgaagttt acgaaggaga
     1561 actaaaaagc tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacgtc
     1621 tgatgaagtc attggatatg ttggctattt cgagttactg aatggattca aaaaaactgt
     1681 ctattggacc aaacaagaaa tcgaagctca tcggattgct aacaataaag atcgcgataa
     1741 gacaaagtta agtggtgtgt gggcatctga ttacaatgca atggcacgaa aaactgtttt
     1801 gagaaatctt ctttctaaat ggggaatctt gtccatcgaa atgcaagaag ccaccacatc
     1861 ggatgagaga gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa cagaagttga
     1921 agaagatatt cctgaaagaa aagaagcaga ggttatatct gaagaaaatg aagatgtaca
     1981 aactggatta tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gctttctccc
     2041 ttgcttttct agaggagaaa tacgaatgaa cacaggatat gtcaaattac atcgaaaagt
     2101 gatgaattca ttcgtttgga ccaatcctta tatgtataaa ttatggaact tgtgtttgat
     2161 gaaagcgagt catgagaatc gcaagatact ttttaatgga aaagagatac aagtgaacag
     2221 cggagaattc gtcacagggc gcgatgctat cacatctgag atgaacgaag gcgtgaaacg
     2281 tgaacatcaa gtgaacagcg gttctgtatg gagatggcta aaacaatttg aaaaaaacgg
     2341 aatgttgaac atcaaatcaa ctacgaaata cagcgttata tcaataaaaa actggtcttt
     2401 gtaccaaagc agtgaacaac aaatgaacat caaacgcaca acaagtgaac aacaagtgca
     2461 cacaaacaag aatgaaaaga attataagaa tgaaaagaat aataaaacat catcgtcgca
     2521 accacgcaaa aagcgtgttt acgacaccga ctcagtttac tacattctcg cggaggagtt
     2581 attcaaacag atttgtcaga atcaggaaat caaaaagccg aatctgcaaa gttgggcaga
     2641 taacattcgg aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac ggggaatgat
     2701 tgaatggagt cagcacaatg tgttttgggc atcgaatatt ttatctgcta aaaaattgcg
     2761 agaaaaatac gacacaatgg cagcgcaagc caatcgtgat tataaaacaa aacaaactaa
     2821 aacgcttgaa tacgagaaat ttagcacaga tgagttgcct atttgagagg aggcgtaggc
     2881 atggaaactg ttggggaaat catggaaaag ctgatacaga aagtgcttgt ccaacgtggc
     2941 gaatgtcctg aatgcggaca gcctttgtat ggatggcgta cgaagaaccc tgatggctca
     3001 gaacgttgta agccaacctg tatgcaatgt gggtataaag cattgcgagt tcaggaagac
     3061 ttacaaacag aacgaattta caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa
     3121 ggtggttctg ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa ttatcaaatt
     3181 gtcgatcaag aaacaagaca agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg
     3241 ttaggaaatc caagtcacct tgttttaact ggaaaacaag gaacaggtaa aagtcaccta
     3301 gcgatggcag cggcttggga agtcttgaag cgatcaaact acgataaaaa aatcttattt
     3361 atcgggttac aggaaatgct ggatcaaatc aaattttcgt acaacaatcc tgaactcaga
     3421 aaaacgattg agggatcgtt gattgcagat atcaaaacag cggatttagt catcattgat
     3481 gatatcggtt cagaactagg aaaagatgca tcagatagtc gagcgtttgg cataaacacg
     3541 ctaaattcgt tcttggatgc acgacagaac ctagcaacga ttatcacaac gaacttgctt
     3601 ggtgaagaac tgaaaaaagc ttacggtacg agaacaatat caagaatgtt tgtcaactct
     3661 gatggattta cgatggtatt ttctcaaaca gcagacaagc gcataaaacc agtcaaagga
     3721 gcgatcgcat gaatttgact aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa
     3781 ttataggtga tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg gttcggaaga
     3841 taagtaagct aattatttat cgggatacag aaagatttct cactgtttta ggtaacgtct
     3901 acgagaatcc agagttattg gaggtagagt aattgccaaa aatattagat gcatgttgcg
     3961 gtagtagatt gttctggttt gataagaaca attctgacgt aacatttatg gattgtcgtc
     4021 agcagtatga agaattatca acaggtcatg tgatcaatgt tgatccagat gtggtcgcag
     4081 actttcgcga tatgcctttt gaagatgatg tgttcgatat ggttgtattt gacccgccac
     4141 acttaagaca tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa ttagacgaac
     4201 tttggccaga agacatcagg caaggttttg cagagtgtat gcgagtttta cgaccttcag
     4261 gaacattaat ttttaagtgg aacgaggaac agataccact ttctgatgtg ttagaggcta
     4321 ttggagaaca gccattgttt ggtaataaac gaagcaaaac acattggctt gtgtttatga
     4381 aaaaattcag ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt attaaataaa
     4441 tgttttggtg gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat
     4501 attgaagtat tcccatacat caataacttt agctatgact cagatgatga atacacgaga
     4561 tacacaggcc aaaagctagg ttcgatggat tttatctact atttcaaaaa agacccgaaa
     4621 atcgacaaag ttacaggaat ttatagcgaa attgaccgac tgtacggaat tgctgatgat
     4681 tccagttttt caagtgattc aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa
     4741 ctaggaagtg aggccagtgg accatatgca agtcttaaag tcgttgatat acccgatggg
     4801 gctgagtggg aaataagtga ttatgacggc gttgaaactg cacattatgg ttttcaaaca
     4861 ggtagtattt agtccactaa tgacaactaa agaggaggca gaaggatgag tgaagatgaa
     4921 tactttgaac agtagtctat taacgacaga gatttttaac tggaaagggg tgtagtcatg
     4981 gacgcatcgg aagtagtaaa taaaattaat agtctcaaag aggttttcgg agacgtcgaa
     5041 gtagtgataa acgcacaagg ctatgacaac ggatttttta agcagattac cgacattagc
     5101 attcagcaag gaatggaaga cgaagatgga aactttatcg atgaagtagc cattttagtg
     5161 acttgcgagt aagtaattga cagctaaaga ggaggcagaa ggatgaaaaa attgcaccaa
     5221 ctcatatctg agaaagaaag cgaactccaa aatttagaag atagtttggg attggggttt
     5281 ccaattgttg agcaagctaa aatgactcaa atcagtcatc tacgacttga attggaagat
     5341 ttaagacaaa tagaaaaatc aattcaactc aacgacaacc agaaaatcgt gttggagtgg
     5401 ttgaaaagcg agacaatttt aactagagaa gcaccaatat tatctgttaa tgctttttct
     5461 gataaaaatt tactaggaaa attacctgat aaagtacgca aagcttataa actattggct
     5521 tgtaaacaag aatatgaagt cctatcagca ttcgctcaat ggggattaga acaggaggaa
     5581 gcggaatgaa caacagacat cgcagaataa caaaactaag aaaacaggaa ctgaatgtac
     5641 taaagacaaa gtttgaaaaa gaatatggaa tttcagcaga agaaacatat aaagtggcaa
     5701 gtcagtttgt tgctgatgcg agtaatgcta ttcgtaagtt tgggatttcg atattaaatg
     5761 atgatcgtaa atgggaggaa atgagatgaa aataaaagac ggattttacg ctagtagtca
     5821 tggtatcggc ggtttaatgc tagacatgcc gataaagaac cctaaaacac gtcagaaatc
     5881 aaaattcaaa atcggtgaca tggtccgatg taaagcagaa gagttcatct atccgtttcg
     5941 tggatatgta gagcatgtct ataatcattc agcaatcatt cacattgaaa acacgatgga
     6001 atgcgataag tggttagcga aaagcaaaga taatttagca gtggctcgtt tggtggatat
     6061 ggaactaatc aatgacaaat aaaaaagccg gatcgctccg actgattcaa taaatccaac
     6121 acatttatta tatcacataa aggagcggtt tgacttgatg caattgttac gagaggtaga
     6181 tttcaaacag acaagatgta atgcgagaga tgtgctgaag aactttcggc gtttggagcg
     6241 gatggcaggt cgctctttga tagatattaa gtcgccgatt attacggata tgccgaaggc
     6301 accgaagcac ggcaataagg cagaagacgc gatcattcag atgatggata tagaagcgga
     6361 gagagacgca atactagcgg ctttgatggc tcttagtctg attagccgtc agatactcta
     6421 ctacagcttc tgtgacgtaa acaagcactc taattatgaa atagggcaat tgatacgagg
     6481 atacggagag aagaatgtag agaagttgaa atccatcgca ttaatcgaat ttgcagaagc
     6541 atacaaaaaa ggcgtgttag ttcagtatcg ttgattttgt agggtttttg tagggatagt
     6601 gtagggtttt tgagcggttt aacgtgatat tatggtagtg tcgaaagatt agtgataggt
     6661 ctgagacaaa ataaaatgta agggaggaaa tctccctcat cgttttaact taagcttcga
     6721 tagacagcaa cggaaatatt aagaataagg atgtgaattt caactccttc taaattgttc
     6781 ttattatcta tcatccgttg ttgtctatta atttatgcat cggaggaaaa acgaatggat
     6841 aaagaaatca aagtaactgt caaattagat ttgactgaac taaaagaact gctcaacaag
     6901 gctagtgacc aagtcgaaca gttacaagaa actttagatg aaattgctaa ttttaaaatc
     6961 caagtttctt agcagtatat tttttagcag ctgcactcat aaaat
//
LOCUS       DAYYAG010000135         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000135 DAYYAG010000000
VERSION     DAYYAG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.138"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHARQ_002514"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHARQ_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHARQ_002515"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHARQ_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8410903.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHARQ_002516"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHARQ_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHARQ_002517"
     CDS             complement(2143..2373)
                     /locus_tag="ACHARQ_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8410904.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHARQ_002518"
     CDS             complement(2603..3421)
                     /locus_tag="ACHARQ_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8410905.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHARQ_002519"
     CDS             3582..4271
                     /locus_tag="ACHARQ_002519"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8410906.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHARQ_002520"
     CDS             4285..5787
                     /locus_tag="ACHARQ_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8410907.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHARQ_002521"
     CDS             5800..6276
                     /locus_tag="ACHARQ_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8410908.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHARQ_002522"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHARQ_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYAG010000136         6608 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000136 DAYYAG010000000
VERSION     DAYYAG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.139"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..878)
                     /locus_tag="ACHARQ_002523"
     CDS             complement(27..878)
                     /locus_tag="ACHARQ_002523"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8410909.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="ACHARQ_002524"
     CDS             1001..1468
                     /locus_tag="ACHARQ_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8410910.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="ACHARQ_002525"
     CDS             complement(1731..2213)
                     /locus_tag="ACHARQ_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8410911.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="ACHARQ_002526"
     CDS             complement(2313..3176)
                     /locus_tag="ACHARQ_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8410912.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            3774..4649
                     /locus_tag="ACHARQ_002527"
     CDS             3774..4649
                     /locus_tag="ACHARQ_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8410913.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(4794..4946)
                     /locus_tag="ACHARQ_002528"
                     /pseudo
     CDS             complement(4794..4946)
                     /locus_tag="ACHARQ_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(5214..6545)
                     /locus_tag="ACHARQ_002529"
     CDS             complement(5214..6545)
                     /locus_tag="ACHARQ_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8410914.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         2105 a         1323 c         1031 g         2149 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     3601 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     3661 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
     3721 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
     3781 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
     3841 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
     3901 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
     3961 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
     4021 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
     4081 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg
     4141 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
     4201 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
     4261 tcatggagat gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct
     4321 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
     4381 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
     4441 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
     4501 tcgatcatac tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact
     4561 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg
     4621 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
     4681 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
     4741 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
     4801 tcatgacaaa tttccttcgt tgctgggtag attttccggc attgttgata gattttatga
     4861 tacttttcta cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga
     4921 caacaacttt caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga
     4981 ttaataatat taacagtctt tatttttcac caccaaaaac ctatctttgc atatactaaa
     5041 tagacgtata tacacagata ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa
     5101 atgtttgttt ttctacatgt tcgtacttat caaaaaatat gatccttatt gaaattactg
     5161 gctatttata ttccaacgac ttcttgtgtt aatttttgtt atctcatagt aaattatgtt
     5221 tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata
     5281 aacacacttg cggctataat taatatttgt aaaacagctg ctcgccagtc tccggctgtt
     5341 gctaaaaatc ccgatataac tggtggcgta gtccaaggaa tttgcactac tacatgatta
     5401 attaatccca cagcagttgc aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca
     5461 aagggaataa tcagactgat attaaataca ataggcaatc caaaaataat aggttcgctt
     5521 atattgaata tagcgggagt taccgataat ttagcaaagt ccttataatc ttttctacga
     5581 ctgaagataa atattgcaat caataaagca atcgtatttc ccgatccacc cattaccgca
     5641 aaggtatctt taaaggccat attgataata tgtggaattt gctctttgtt tgcataagct
     5701 gccatatttt cttgcatatt ctgaatcaaa aacggatcta acaatgaacc tgatattact
     5761 gcttgatgga taccaaatcc aaaaaataga ttagcaatag atgtaattaa caaaaatcct
     5821 ggtaaaccag ttgtaatttt acttaatggc tctgtaacta tcgtggtaat tagcttattt
     5881 atatccatat taaacaattg aatgattata aatgacaata aagcaaacga ggtaatcgta
     5941 ataatgattg ggattaatac attaaaagac tttaaaacag cgggaggaat atttccggat
     6001 agctgaatct gtagtttctt attattagaa agtttaataa ataattctgt agccgctaat
     6061 ccaacaaata tacctacaaa cattcctgaa gctcctgtat aggatatagg tatcacaccc
     6121 gtcacttcaa cagctttatc cgcattttca ggagtaaaca tcatctgcat aggtgttaat
     6181 acaacaaatg tcgaaatagc aactaatacc gctgcaatat gatttgtata atttctgctt
     6241 gcacacatat gataagatac tgctgctaca actaataatg tagttatgct taatgtacca
     6301 ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg tactagtatc gacaatttcc
     6361 cccatgaatc ctgttggttc taagatcact gaattaataa gtgtcacaaa tcctgctaac
     6421 atcataaatg gcataaatgt agcaaacgca tctcgcaaag ttcttaaatg gatctgattc
     6481 cccaacttag tcgcaactat accaaatttt tcaaatacaa tgtccttcct gctacttttg
     6541 cccatatttt ttctccttta tgcttattgt aatcgattac tttttccacc caaataatac
     6601 atcactta
//
LOCUS       DAYYAG010000137         6599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000137 DAYYAG010000000
VERSION     DAYYAG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.140"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHARQ_002530"
     CDS             complement(1273..1938)
                     /locus_tag="ACHARQ_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8410915.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACHARQ_002531"
     CDS             2064..4100
                     /locus_tag="ACHARQ_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8410916.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHARQ_002532"
     CDS             4104..4742
                     /locus_tag="ACHARQ_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8410917.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHARQ_002533"
     CDS             4770..5198
                     /locus_tag="ACHARQ_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410918.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHARQ_002534"
     CDS             5655..6566
                     /locus_tag="ACHARQ_002534"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8410919.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2269 a          999 c         1219 g         2112 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaat
//
LOCUS       DAYYAG010000138         6517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000138 DAYYAG010000000
VERSION     DAYYAG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.141"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..774
                     /locus_tag="ACHARQ_002535"
     CDS             37..774
                     /locus_tag="ACHARQ_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8410920.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            788..1603
                     /locus_tag="ACHARQ_002536"
     CDS             788..1603
                     /locus_tag="ACHARQ_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8410921.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1616..2263
                     /locus_tag="ACHARQ_002537"
     CDS             1616..2263
                     /locus_tag="ACHARQ_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8410922.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2279..2935
                     /locus_tag="ACHARQ_002538"
     CDS             2279..2935
                     /locus_tag="ACHARQ_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8410923.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3097..3918
                     /gene="racE"
                     /locus_tag="ACHARQ_002539"
     CDS             3097..3918
                     /gene="racE"
                     /locus_tag="ACHARQ_002539"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8410924.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3921..5276
                     /gene="rph"
                     /locus_tag="ACHARQ_002540"
     CDS             3921..5276
                     /gene="rph"
                     /locus_tag="ACHARQ_002540"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8410925.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5277..5795
                     /locus_tag="ACHARQ_002541"
     CDS             5277..5795
                     /locus_tag="ACHARQ_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8410926.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5902..6402
                     /gene="cbpB"
                     /locus_tag="ACHARQ_002542"
     CDS             5902..6402
                     /gene="cbpB"
                     /locus_tag="ACHARQ_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8410927.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2145 a         1095 c         1441 g         1836 t
ORIGIN      
        1 agaaatctta ctaaataata atgggagtgt aattctatgg caatgattaa atttgatcac
       61 gtggataaat attatggcaa gttccacgca ttaaaaaata tcaatctcga attcgagaaa
      121 ggagaagttg tcgttgtcat tggtccttct ggttcaggaa agagcacgat gcttcgctgt
      181 atcaatggat tagagacgat ctcttcaggt aagctgctga ttaatgatac tgacttgcat
      241 gataaaaaga caaaactgac agaagtccga aaaaacatcg gcatggtttt ccaacatttc
      301 aatttgtacc caaataaaac tgttctggaa aacatcaaat tagcaccaat caaagtgttg
      361 aagcaagacc aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac tgtcaatatg
      421 ttagataaga aagattctta tccttccatg ttatccgggg gacaacagca acgggtggca
      481 atcgccagag gtttagcgat gaatcctgag atgctgctgt ttgatgaacc tacttctgct
      541 ttggatccgg aaatgattgg tgatgtatta gacgttatga aaaaattggc tcatgacggt
      601 atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc ggatcgtgtt
      661 atcttcatgg ctgatggaga agtattggaa gacagcagaa atgtgagaga ctttttcgaa
      721 aatccaaatg aagtccgagc acagcaattt atcagtaaag ttatcaatca ttgataggag
      781 gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc cttttaggca ttgcttttct
      841 tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag agcgaagcaa
      901 atctaccaat gaaatcatct ggggtgtcaa gtatgatact cgattatttg gaatgatgga
      961 tattgagagc agaactgtac aaggatttga tgtagatatt gcaaaagcaa tcactaaaaa
     1021 gatccttggt gacaatggaa aaacagaatt tgttgaagtc acttctaaaa ctcggattcc
     1081 attattgaaa aacggaaata tcgatgcaat tattgcaacg atgacgatca cagatgaacg
     1141 aaaaaaacag gttgactttt cagatgtcta tttcgatgct ggccaagctt tgttggtaaa
     1201 gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct tctacaacgg ttctagcagt
     1261 caaaggttct acatcggctg cgaatatccg acagcatgca ccagatgcaa aaattttaga
     1321 attagaaaac tatgcagaag catttactgc tttacaatct ggtcagggag acgcgatgac
     1381 tacggataat gcgattttgt tagggattgc agatgaaaat ccagagtatg agttagttgg
     1441 tggaacgttt actaatgagc cttacgggat tgcgatcaac aaagggcaag aaaacttctt
     1501 gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat ggcacatacg ataaaatcta
     1561 tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag taagggggag aggcaatgag
     1621 cgaattgata caaacttatg gaccaatctt tgtcgacggg ttcaagatca cattgttttc
     1681 gagtgtctta gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa tcttccaatt
     1741 atcaaagaat aaagtagtga gcgccttagc aaaggcatat gtagaatttt ttagaaacat
     1801 tccattatta atcatcgtga tgttttttta tgttgtcgta ccgatttatt ggtttaatct
     1861 agatggattc caagcaggta cgattggatt aacgatctat acatctgctt ttattgctga
     1921 aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa acagaagcag gactatcttc
     1981 tggttttacc tattcagaaa tcatgcgtta tattgtttta ccgcaagcat tcaaaatcgt
     2041 tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta ttttagcaat
     2101 cgtagctggt ttagatttga tgtatcaagg agacttgatc gctagtgaaa cattcaatac
     2161 cttcgatacg tatatcatcg ttgggttatt ttacttagtt atcacgctac cgttatctta
     2221 cttaatggtt tatcttgaga aaaaatgggc ggttcgttca tagaaggagg ggaaatacat
     2281 ggattttagc ggcgcttttt catggatgaa tatccgtttt ttattggaag gattaaaggt
     2341 tactgttgaa gtttcattgt tttcgatcat ttttagtttc ttgatcggtg gtctgacagg
     2401 tgtgattcgt tttgcgaata tccctttctt ttccaaaatc cttggtgtag tgattgatat
     2461 tatacggaat cttccattat tattgatcat tttcttcact tactttgcct tacctcaaat
     2521 cggcattcag atgaacatct tctggtccgc tgtcgcagca ttaacgattt ttgagtcagc
     2581 tatgttgtcg gaaattttcc gagctggact caatgctgtt ccaaaaggac agatggaagc
     2641 aggattatca acgggactga cttatgtaga atcgatgcga acgatcgtgt taccgcaagc
     2701 atttaaatca atgatcccgg caattgtgag ccaattgatt tcactgatca aagatacttc
     2761 attagcagta atcatttctt tacctgaact ttctcatcag gcacgtatta tttatggaca
     2821 gaacaccaat tatgtgcttc cgatgttcat catcatgaca cttatgtact tcgtggtatg
     2881 ttacgcgctt tctctgcttt ccggttactt ggaaaagaga cggtacagtt attaatagat
     2941 gattctttta gcggttgtgg caaactaaag tcacaaccgc ttttcctctt tccattcgaa
     3001 aaaaagagat ttcgattgtt ttaagcaagt aagagcactt gaaaattcac gctaaaaagt
     3061 acacaatagg gaggatagaa tttattgaaa aaaaggatga tacgattgac agataatcgc
     3121 cctatcggat ttattgattc aggtgtcggc ggcttgactg tagtaaaaga agccctgaaa
     3181 caattaccga atgaaaatat tttatttgta ggagacacag cacgctgccc atatggccct
     3241 agacccgcgg aacaggtaat ccagtatact tgggaaatga cggattatct ggtggagcaa
     3301 ggaatcaaga tgctggtgat cgcctgcaat accgcaactg cggtggcttt agaagaaatc
     3361 aaagctgctc tttctattcc agtcatcggt gtgatccttc ccggtactag agcggcagta
     3421 aaaaaaacac aaaataaaca agttggcatt atcggtacga ttggtacggt aaaaagtcaa
     3481 gcttatgaaa aagcactgaa agagaaagta ccagaattga ctgtgacaag tcttgcttgt
     3541 cccaaatttg tttcagttgt cgaaagtaat gaataccatt catcggtggc gaaaaaaatt
     3601 gtggcagaaa cattagctcc tttaaccact aaaaaaatcg atacattgat tttgggatgc
     3661 acccattatc cattattacg ccccatcatt caaaatgtaa tgggagaaaa tgttcaactg
     3721 atcgattctg gagcagaaac agtaggtgaa gtatctatgc tgttagatta tttcaatttg
     3781 agcaattcac cgcaaaatgg tcggacatta tgccagtttt atacaactgg ctctgccaaa
     3841 cttttcgagg aaatagctga agactggctt ggaatcggac acttaaatgt agaacatatc
     3901 gaattgggag gaaaataagg atgcgtcatg acgggagaaa agcccaagag cttcgcaaac
     3961 tggagcttca aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc cgttttggtg
     4021 atacaacagt tatttgttct gcaacaatag aaaattctgt tccccctttt ttgagagaaa
     4081 caggaacagg atgggtgaca gcagaatata gtatgctgcc tcgagcaacg agtacgagaa
     4141 atcgtagaga aagcagcaaa gggaaacttt ctggtagaac aatggaaatc cagcgtctta
     4201 ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt aggagaacgt agtatcatcg
     4261 tagactgcga tgtgatccaa gcggatggcg gtactagaac agcaagtatc actggggcat
     4321 ttgtggcatt gaaattagcc attgaaaaac ttttaagaga aaaagaattg tcagaagatc
     4381 caataaaaga acatcttgct gcagttagcg taggaatctt accagatggg acttgcgtca
     4441 cagatttgga ttaccaagaa gattccgcag cacttgtaga catgaatctt gtaatgacag
     4501 aatctggaaa attcgtggag attcaaggaa ctggagaaga agcaacgttt gatggtgaac
     4561 agttgaatga aatgctcttt tttggaaaaa acgcgattga agatttgatc aaagagcaaa
     4621 aacatgcttt gctaacagag tttgctcaaa atgatgagcg gatagaagaa acaaaaacaa
     4681 tcatcattgc gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg ttcaaagaag
     4741 cgggatatca tgtaaaaaca ttacttgact atcctgaatt gccagatgtg gaagaaactg
     4801 gcagcacatt tgaagaaaat gcccgattaa aagcagaaac gattgcacaa ttgcttgatc
     4861 agccagtctt agcagatgat tcaggtttga aagtggatgc tttaggcggt atgccaggaa
     4921 tctattctgc acgttttgca ggggaacaaa aaagtgatgc ggggaataac gccaaattat
     4981 tatatgaatt gacagatgtg ccggatgaaa aaagaacagc acaattccat tgtacgttag
     5041 tattcgcggc acccaaaaaa gacagccttg tagtagaagc ggaatggccc ggtcgagtag
     5101 cccgtatacc atctggcgaa aatggctttg ggtatgatcc tttatttatc ccagaaggta
     5161 agaaacaaac agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt catcgggctc
     5221 aagcaatgaa aaaattgagt gccgagtgga aacaatggct ggaaggagag agatagatgc
     5281 ggtatttagt agtaagtgat aatcatggag acagagatat cctcgttgac ttagtagatc
     5341 gttatcgttc tgaagtggat atctttttcc attgtggtga ttcggaatta gaagcttctg
     5401 acccattatg ggatacattc caagtggttc aaggtaattg tgattatgga ccaggattcg
     5461 aacagaaaaa agtgatccaa actggtcagg atacagtatt catgactcac ggtcatttat
     5521 cgaatgtccg atttggtttg acacagttag caatcgaagc tcgagctgca actgccgata
     5581 tggtgttttt tggtcatacc catcaaatag gctgtgagat ggatcagaaa atcctgtttt
     5641 tgaatcctgg tagcatttct cagcctagag gcccgatcca aatcccatca tatgctctta
     5701 tcgaaagttt agatgaccaa atttctgttc aatactataa ccgtgcccat caggcagttg
     5761 aagagttggc cttttctttt tcgagagagc gctgaataaa aaaactctaa tttttaataa
     5821 aaataagctt tattttatga attttgcatc cggtttcggt aaaatagtag tgttgaatga
     5881 aatagaaatg gaggccatca gatgattgga cctatagtaa gagaaatgct gctggaaaat
     5941 caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca tcatccattg
     6001 tcccatggtt tacttgtgtt atcgaaggtc ggttattcca aaattcctgt attaggaaaa
     6061 gatgatcact ttgtaggact agtaagttta tcagatgtgg tcaataaaat gattgacttg
     6121 caaacaatca gcatggaccc gttagaagga ctaacggttg ctgatgtgac agaaacagat
     6181 gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt agtcgattcg
     6241 ccttttttac cggttgtcac agaagatcgg attttcaaag gaatcattac aagaaaagag
     6301 ctgcttaaag ccgtaaactt catggtgcat gaattagaac gtcgtaatat attgtctccg
     6361 aaaatcgaag aattaaaaga acaaatcgat atcgtgagct aaagaacgag aaactaaaag
     6421 ataatcaaaa aataagtaga aaaaacagcg taccagtttt gactttatct tgaaaaagag
     6481 gctgggacaa aagcgtttaa ctccaagaaa taagaag
//
LOCUS       DAYYAG010000139         6362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000139 DAYYAG010000000
VERSION     DAYYAG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.142"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..368)
                     /locus_tag="ACHARQ_002543"
     CDS             complement(18..368)
                     /locus_tag="ACHARQ_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8410928.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(383..2110)
                     /locus_tag="ACHARQ_002544"
     CDS             complement(383..2110)
                     /locus_tag="ACHARQ_002544"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8410929.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(2324..3250)
                     /gene="trxB"
                     /locus_tag="ACHARQ_002545"
     CDS             complement(2324..3250)
                     /gene="trxB"
                     /locus_tag="ACHARQ_002545"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8410930.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(3395..4828)
                     /locus_tag="ACHARQ_002546"
     CDS             complement(3395..4828)
                     /locus_tag="ACHARQ_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8410931.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(4828..6231)
                     /locus_tag="ACHARQ_002547"
     CDS             complement(4828..6231)
                     /locus_tag="ACHARQ_002547"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8410932.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         1679 a         1479 c         1091 g         2113 t
ORIGIN      
        1 tctatttgca cctatcgtta ttgatgttcc acatgtttga gtgtctggtt caaaatatcc
       61 agtacatgag aatcatcaag actatacaaa atcgcttttc cctcgcgatg agacttgacg
      121 acatgatttt ctcttaataa acgtagttga tgcgatacag cagactgctc catttccact
      181 gcttgggtga ttgctgtcac attttgttcc ccgtctttca gatatagcag aattttaaga
      241 cgagtggggt cactcaacat tttgaataat tgactgactt tctctacttt tgatttttgc
      301 gtgtctggca atccattcac ctccaacttg aaaaaagact gacttctaaa gtttaccact
      361 ttttccatca gtctcaagtt ttttattctt ctgtcatctt tttgataatc gcttcataag
      421 aggctacttt ttcggctgcg tcttctgtag agcttccttt tacaccaata taaaacttga
      481 ttttcggctc agttcctgaa gggcggatag cgatccagcc attttcttcc aatgtatact
      541 tcaatacgtc ggatggcggt gttgttagtt tttctacgtt tccttcacgg tctgttctcg
      601 tcaattcttt aaagtcttct gtttgaataa cttttgctcc ggcaaattct gtcggtgctt
      661 ctttacggaa tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg
      721 tcacagaaat cgttttttct gcgaaatagc cataggtttc aaaaatcgtt tgcaagccgt
      781 catacaacgt tttgccttgt tttttatagt aagctgctac ttctgctaat aaaagcaatg
      841 cttggatcgc gtctttatcg cgaacaaaag gtttaactaa ataaccataa ctttcttcga
      901 atccaaacat aaaagtatgt gaatggtctt cttcaaattg ttggattttt tcagcaatga
      961 atttaaaccc tgtcaacaca ttgaacatag tagcaccgta gtgttccgca attgctgtag
     1021 gcaattcgct ggaaacgatt gatttcaata ccgctgcgtt ttctggtaaa gtgcctcttg
     1081 attgatgtgc ttccaaaata tattgagcaa acaatgctcc gatttggttc ccagtcaata
     1141 cttcataaga accatctggc aaacgaaccg ctgcgcctaa acggtctgca tcaggatctg
     1201 ttgcaatcag caagtcagct ccctcttttt ctccaagtcg tatagcatat tcaaaagctg
     1261 aatgttcttc tggatttggc gatttgaccg tagtgaaatc tggatctgcc acagcttgtt
     1321 cgggaactag tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt
     1381 tccctgttcc atgaagtggc gtgtacacaa gtttcaaatc tttccccatt gtttcaacca
     1441 attcacgatc aatcgtcact gctttgattt cttctagata agcagcgtcc acttcttctc
     1501 cgatgatcgt gatcaacccg ctatgtttag cttcttcgtc agataatact tcgactttta
     1561 gcggattgct gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt
     1621 gtccgccatc ttctccgtag actttatatc cattgtaagc cgctgggttg tgagatgcgg
     1681 ttaccatgat tcccgcaaac gtcttcaaat gacgaactgc aaaagaaagc tctggtgttg
     1741 gacgcagact ttcgaataca taagacggga tattgtgatg tgccagtgtt ttggctgctt
     1801 ccattgcaaa ttctggtgac tgatggcgtg agtcataagc gatagcgaca cctcggcgtt
     1861 ttgtttcttc accttgtgta tccatgaaac gtgctagtcc ttctgttgct tgtcttaccg
     1921 tatagatgtt cattcgattg atcccaggtc ctaagattcc ccgcatgcct gcagtaccga
     1981 attccaacgg tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta
     2041 attgtttttt taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt
     2101 cccaagacat gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca
     2161 cttattagat atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta
     2221 tcaagcatga ttgcaagtat tcacataaat cggattcagt ggatttcacg cgaaaaaaaa
     2281 gagtaaaaga acaattgaaa ccgttctttc actcttttta aacttatgct gttgtttctt
     2341 ctaaagactc caagtattta tacacttgtt gtccagcaat accgccgtca ccaacagctg
     2401 tagtgatttg tcgcaaatct ttttcacgaa catctccgat ggcaaaaacg cctggcatat
     2461 tcgttttcat ttcgttgtct gttggaatcc agccagcttc gttcgtcaaa cctgcttctc
     2521 taaaaggtgc agttaatgga tccagtccaa catagatgaa tactccgtct gctgctaaat
     2581 caaattcatt gccggttttg acattacgtg ctttcacacc tgtcactacc atttcgtttc
     2641 caacgatttc gtcaacgact gtatcccaca taaaggaaat tttttcattt gcaaatgcac
     2701 ggtcttggat gatcttttgt gcacgtaatt catctctacg gtggacgatc actacttcag
     2761 aagcaaattg tgtcaaatag atggcttctt ctactgctga gtccccaccc ccgatgacta
     2821 ctagacgttt gttacggaaa aacgcaccat cacagactgc acagtaagaa acgcctcgtc
     2881 cagcaaattc ttcctcgcca ggtacgccta atttacggtg gatacagcct gttgcgataa
     2941 tgactgtttt tccttcgtaa cttttatctt cacagatgac ttctttatgg ctgccgtgat
     3001 ccttgatatc ttgaacgatt ccataagcat tttctgtccc aaatcttgag acactttcat
     3061 acatcttata agctagatcg gggcccatga tcgaatcgaa tccaggataa ttttcgattt
     3121 ctgccgtatt gttcatttgg ccgccagggg caccttgttc gatcatcaat acggataagt
     3181 ttgaacgtga agcatacaaa gcagcagtca tcccagcagg acctgcgcca ataacgatta
     3241 catcatacat atgtagattc tcctttactt aatcaagtaa gcatacttta cagccaatgc
     3301 gaaaaaaagt ctattgttct gcttgcgttt ctcgaatagc tttattatac tcgaaaaatc
     3361 cgctgaaaaa caatagactt ttataaatca ttgatcaatt gttcgaaaga ttttctccat
     3421 ttgaatcaat tactttttta taccagttga atgatttttt ctttgaacgg gctaatgtgc
     3481 catttccttg gttatcacga tcgacataaa tgaatccgta ccttttcttc atctcaccgg
     3541 taccagctga tactaaatcg atacatcccc aagtcgtata gcctaataga tcgactccgt
     3601 ctagttctac tgcatccttc atcgcctgga tatgcgctgc caggtaatcg attcggtaat
     3661 catcttccac ataaccattt tcatctggcg tatcgactgc acctaagccg ttttctacaa
     3721 taaataatgg tttttgataa cgatcataaa tatcattcat cgttattctc aagcctaatg
     3781 gatcaatttg ccacccccat tcacttgctt gcagatatgg atttttgaca gaagcaaaga
     3841 tatttcctgc agtctgttcc aagagttcgt tgtcggtagt cgaaactcgt gaagaataat
     3901 aagaaaaaga gataaaatca acagtatgtt ctttcagcaa tgcggcatcc cctggttcca
     3961 tttcgatctg aatgccattt cgctccattt ctttcaatgc ataagcggga tattctcctc
     4021 gtgactggac atcaatgaag aaataatttt ctcgatctga ttttctagaa gcccaaacat
     4081 cttcaggatt gcaagtgtat ggataatatg ctcctgctgc cagcatacag cctactttgt
     4141 tctccggatc tacttcatga gcaatcttcg ttgcgatggc acttgcaacc aactcatgat
     4201 gtgccgcttg atatttgatt tgttcttcat tttctccttc ttcaaaatac agccctgcgc
     4261 ccataaatgg tgcatgaagg atcatattga tctcattgaa tgtcagccaa tatttgacaa
     4321 gtcctttata gcgattgaag atgactcgac acagattttc ataaaaacca acgagttctc
     4381 tggaacgcca cgcaccatat tctttgatca aatgcattgg acaatcaaaa tgcgtaatcg
     4441 tcacaagtgg ctcaattccg tgcttttggc attctttaaa gacatcctca taaaatttca
     4501 agccagcttc attcggctct ttctcatccc ccattggaaa aatacgactc caggcgatag
     4561 acattcgata agtcttgaac cccatttctg caaacaaagc aatatcttct ttataacgat
     4621 gatacatatc gattgcaatt tttgctggat aaaaatgatc ctcatcaaat tcaaacattt
     4681 ttttctttcc ggtgataaca ggaaaacggt cttttccgat tggcacaaca tctacgtttg
     4741 ctagtccgcg tccaccttca tcataagcgc cctcacattg attggctgct gtagcaccgc
     4801 cccataaaaa atcttttctg aaccccatta tacttcctcc attatatgtg tattacctga
     4861 taattctctt gccagttcat cttgaacttc gattccttct tcttttaatc ggctgattgt
     4921 ttccttgaat tgcggtaaat actgctggtg tgcaataaac aactcatcca tgatccgttt
     4981 tgctgtcttt cctgatcgta taagtggatt gatcgtaaac gcctgtaaag ctgtcccata
     5041 atcaccagtc acagctgctt cgatcgtcag caattccata ttcttcatta cttgcaacca
     5101 gcctctttca gcagcaggaa gagaaccaaa cgctacattt ctcgctcctt gtgcgccaac
     5161 ataggcagtg acttctacaa cacaatctgc cgacaggtca ggaacagctc catcattctt
     5221 tgtagataca acgatctgtg tttctttatt tccatagatt gaagcaatcg tttcacaggc
     5281 agcgtctgaa taataagcgc cacctcgttt ttgcaattgt tcaggtttat ggtctaattc
     5341 tggatctttg tataattcaa atagctctgc ttctgtttct ttcacttgct gcgctcttgt
     5401 accaatcgtt tgatattctt ctaaagcatg ttccagcatt tcttcttcac gatagtaata
     5461 gcggtggtag ccacaaggga tcatgttcat ttgttttaat tgttctttga aaaacgggat
     5521 atcaaagata tttttcggta atccattatc ttcatcgtat aaacgatcaa tcaattctgc
     5581 cgtgcggtca tttcctttat tatctgctac tttgtgccaa tggaaatgat ttagaccggc
     5641 aaacttgtag atcagctctt cttttttctt gccaagcatt gctggttctg tcatcatcgc
     5701 catcactggc acattgcata atccaatcac tttatcccat ttgccatatc tgataactgc
     5761 ttctgtcacc atcccacttg ggttcgcgaa attgatcagc caagcatttg gacataaacg
     5821 cttcatgtcc tcaacgattg ccaaaacgac tggaacggta cgaaaagctt tgaatatccc
     5881 gccagcacca tttgtttctt gcccaatcat cccataataa ctagggatcc gctcgtcttt
     5941 gattcgtgca tctaattgac cgacacgaaa ctgagtcgta acaaaatccg catctttcaa
     6001 tgcttcttcc cgatccagag ttgcatagat cttgacttca tatggagaag catcccacat
     6061 acgctgagac atctgactaa cgatctcaag cttttctttt ccttcttcta catctacgag
     6121 ccagatttct cgtatcggta attcctcata tcttttgata aatccttcca tcaactcagg
     6181 ggtgtaactg ctgcctccac caatcgtagc gattttgatt ccttttgcca tgttctcact
     6241 cctttacttt tttacacttt cattatcttt tttgtaaaca aaacaaacaa tagaaatccg
     6301 tttctttcca gtaacattta caaaagttac tgttttctag tggatttttg taaatatctt
     6361 gt
//
LOCUS       DAYYAG010000140         6253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000140 DAYYAG010000000
VERSION     DAYYAG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.143"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHARQ_002548"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHARQ_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8410933.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACHARQ_002549"
     CDS             500..862
                     /locus_tag="ACHARQ_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8410934.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACHARQ_002550"
     CDS             914..2596
                     /locus_tag="ACHARQ_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8410935.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACHARQ_002551"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACHARQ_002551"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8410936.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACHARQ_002552"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACHARQ_002552"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8410937.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACHARQ_002553"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACHARQ_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8410938.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2075 a         1090 c         1427 g         1661 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa atgctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttc
//
LOCUS       DAYYAG010000141         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000141 DAYYAG010000000
VERSION     DAYYAG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.144"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHARQ_002554"
     CDS             725..811
                     /locus_tag="ACHARQ_002554"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8410939.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHARQ_002555"
     CDS             complement(932..1621)
                     /locus_tag="ACHARQ_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410940.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHARQ_002556"
     CDS             complement(1866..2780)
                     /locus_tag="ACHARQ_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8410941.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHARQ_002557"
     CDS             complement(2762..3142)
                     /locus_tag="ACHARQ_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8410942.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHARQ_002558"
     CDS             complement(3592..3879)
                     /locus_tag="ACHARQ_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8410943.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHARQ_002559"
     CDS             complement(3899..4123)
                     /locus_tag="ACHARQ_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8410944.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHARQ_002560"
     CDS             complement(4578..4748)
                     /locus_tag="ACHARQ_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410945.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHARQ_002561"
     CDS             complement(4763..5296)
                     /locus_tag="ACHARQ_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8410946.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHARQ_002562"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHARQ_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8410947.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYAG010000142         6107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000142 DAYYAG010000000
VERSION     DAYYAG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.145"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..847
                     /locus_tag="ACHARQ_002563"
     CDS             <1..847
                     /locus_tag="ACHARQ_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8410948.1"
                     /translation="LIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREF
                     WNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKN
                     AFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWK
                     ATNPKKEQEAMKLKNTKSNNLLYAMCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNW
                     KFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVL
                     KKIAL"
     gene            867..1427
                     /locus_tag="ACHARQ_002564"
     CDS             867..1427
                     /locus_tag="ACHARQ_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFM8410949.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2316..3083
                     /locus_tag="ACHARQ_002565"
     CDS             2316..3083
                     /locus_tag="ACHARQ_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFM8410950.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3109..3654
                     /locus_tag="ACHARQ_002566"
     CDS             3109..3654
                     /locus_tag="ACHARQ_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410951.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3655..4221
                     /locus_tag="ACHARQ_002567"
     CDS             3655..4221
                     /locus_tag="ACHARQ_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410952.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4236..4484
                     /locus_tag="ACHARQ_002568"
     CDS             4236..4484
                     /locus_tag="ACHARQ_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410953.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4503..4742
                     /locus_tag="ACHARQ_002569"
     CDS             4503..4742
                     /locus_tag="ACHARQ_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410954.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4759..5343
                     /locus_tag="ACHARQ_002570"
     CDS             4759..5343
                     /locus_tag="ACHARQ_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410955.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5450..5731
                     /locus_tag="ACHARQ_002571"
     CDS             5450..5731
                     /locus_tag="ACHARQ_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8410956.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5721..6062
                     /locus_tag="ACHARQ_002572"
     CDS             5721..6062
                     /locus_tag="ACHARQ_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8410957.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2283 a          798 c         1271 g         1755 t
ORIGIN      
        1 attaattaaa aaggatgaga ttataatggg gttactggtt ccgagagcta atacttatac
       61 tataccttta acgaaacgcc aattaattgc taaaagatat caaaggatta ctagagccat
      121 caatagagag ttttggaatt ctgaaagtga tactgctcac agtttatacg taggttctta
      181 tgggagagga acggcaatta gtactagtga tatagatata atagttgaat taccgatggc
      241 agaatttgac agattcaaaa attatttatc aaatggcccg tctaagttat tacaagttat
      301 aaaaaacgca tttcaggaaa tactaccaaa tagtgatatt agagctgatg gacaagttgt
      361 taaaataaat ttccatgatg gaatcaaatt tgaaatagtt ccagctttta atgaaaaaga
      421 ttattggggt gagtcaaaag ggtttattta tccagattca aacatgggag ggaactggaa
      481 agctacaaat cccaaaaaag agcaagaagc catgaaatta aagaatacaa aaagtaataa
      541 tttattatat gctatgtgta aacattttcg acatgtacga gatacagaat ttacgagtta
      601 tcatctttca ggaatagtaa ttgacagttt cgtatatgag gctatgggaa attggaaatt
      661 cgttgaaaat aacagtgggg gtcaaaacat tagttctgta tcttatgaga cagctttatt
      721 agaatactat aactcacata aagttatggg cggtttaaat ttatactctc ctggaagcaa
      781 tcaatttgtt aattctgata gtagtatcat atgtttagaa aaagtattaa aaaaaatagc
      841 tttataaagt tttatggagg aacaacatgg gagacgaaaa aaagcgcttg gaagttcaat
      901 tacgtgaagc atatggacga gtaacgtata cttacaccac tcatttaaag caagccgata
      961 atattatttc tgacaataaa attttaaggt gtcttcaaat tggtttgtca gcccttacta
     1021 ctggaggggt acttagtacc attattactg ataatagtga gttaaccttt gttgctggat
     1081 tatgttcaac aatttctctt ggattaaact tatactttaa gaattttgat cttgctgaag
     1141 aagctaaaaa tcatcaaatt gttgccgata aattatggtt gatcagagaa gaatacgtaa
     1201 gcttactaac agatttaaat cgtttaagtg ttgaagatat tgtgaaaaaa agagatcaat
     1261 taatgaacga aactcatgaa gcatataaaa atgcccctaa aacaaatagt aaaagctatg
     1321 aatcagctca gaaagcatta aaatctgaag aagagcaatt ttttacagaa gaggaattgg
     1381 ataagatgtt accagagcat cttagaaaaa agagttctaa atattgaact ctttttttat
     1441 gcttaaaatt ctcttgtaat ttttctattc atctatattt aagaagaaat atgatgatag
     1501 aaacatctcc taaagccacc gcaagccgtt ctaagcatag cttagcccga cattgctaga
     1561 tatgctcatc tcaccatgaa cgtttcctaa agccaccgca agccgtccta gccattgcct
     1621 agtccgacat cgctagatta gtgcatctcg ccataaaagt tttctaaagc caccgcaagc
     1681 cgttctaagc atagcttagc ccgacattgc tagatatgct catctcacca tgaacgtttc
     1741 ctaaagccac cgcaaaccgt cctagccatc gcctagtccg attttgcttg atcggttcca
     1801 tttctgttta tttagtgggt caacaccgta atcttaaata gccatcgcct atttaagatt
     1861 actaggtctg ctttgaaggt aaaaagtctt tcattcttca agacttttag gctttcaagc
     1921 tacgcctgaa agcattgaaa ttcatcgctc tttatttggt tttgccattt ataattcctt
     1981 acgcccatta tctttttcgc ctgtcatacc ttgtcagaag tgtttagagc ttatctcatc
     2041 tttggattaa tacaagtggc gtaaattttt ttctaaacgc aagcgttacg aaaataattt
     2101 acttaaacac ttgtattaat ccggcagata agaacgcatt ctaatcactc ccgacaaggc
     2161 aagacaggcg aaaaagataa ataaatgaac gaaaggaaga aacataaaat gcaaaaccaa
     2221 atagcgatga agaaaaaacc acctccctct aaaacaatcc aaacaaaaaa gaattttgaa
     2281 aaaccaaaaa tcaaaaacag aaagaagcgt tcaacatgag agatagaatt ttagaagtta
     2341 atcaagaaat ggtaaacaag ttaacaaata tcagtgggaa ctatgaaaag tacgatggta
     2401 ctttacttga taactatatt tttcacgata cacaaaatat cagaattggc agaagtaaac
     2461 cgagagcgtt cattctcatc aaagagaaat atttgaatga atggaatagc acctatgaaa
     2521 tggttatgac tgataacttt gaaaaggtat tggatttcag aaatgctttg ttgggttctg
     2581 atatgggaaa tagcagtctt gaatctattg aagttgaaaa caagaccgag aatgaaggtg
     2641 aaggattgat taagaatgtc ttgaaaatcg aacaaaaata tatcagtgaa gatagaagtt
     2701 tttatagagt cacaagctcg gttggtctag caaactttgc tactaactta attggaaaca
     2761 atgctagtga atctttgcta gtgatgtgtc tgaatacaaa gaatgaggtc attgcttata
     2821 gcgaagtttt caaaggtgga attaatcaat cagtagcgat accaagagag atttttcaac
     2881 tagctttgct aaacaattca gcaaaaatcg cagtatcaca taatcatccg agcggatatg
     2941 aatttccaag tgaaaatgac aaaagtttta ctacgaattt gaaaaacgca ggaaaaacaa
     3001 ttggtattga attgttagac catatcatcg taacaagtaa aaacacaagc tattactcat
     3061 ttcaagaaaa tggttcttta taaaaattag agaaaagagg aaaacaagat gggcgaaaga
     3121 acacaagcgt taattacagt caaatatggt aacagcaaaa cgaaagcaag cgtgtcgatt
     3181 cattatcagt gggggtatgg tcgatatatg ctgatggacg tattgaatca aggaatttat
     3241 ttaatcaata aagcatacga cttacgagat aaatcaagag aagaattagc aaaaggaatc
     3301 tttgatagaa gtacaggagt tatcaatttt gtcacctatg aatctggagt gagtgcttat
     3361 gatgaaacag ggtttggtgg gcgctatgaa ccttatcaag aaacgattga agatgtgatg
     3421 aagttttcag ataacaatga tggctatgct catttagaga tggtgttcga tgaaaatgga
     3481 tttagtctag atgataaaac cagattgcga ttgtttgatt ttgagggtca acagatagga
     3541 ctaaacaagt tttgtgaaag cggaaaagag tttgctacga aagattttat taagggatac
     3601 agattattgc tagaaaatta tggaataaac ctagaagaag agagcgtgaa ataagtgttt
     3661 gaaaaaagaa aagaccagtt ccctaattta gaagcgaaaa gtcaaaaagt gattgatggt
     3721 gaaatgattg aattattcca aaaaagttat ggaaccatag aaactaccgc aagttgcaaa
     3781 tactactttc aaggaaagag aaacacgttt aaaggctttg ccgattctga tgaagaattg
     3841 gcaaagaaaa tcattgattg gcttgtcagc agtgaacacg tagcaagaga agataccgtt
     3901 agaacaacga ttaatgggga agtcctaaca ggtactagat gtgtttgttg taatcatgaa
     3961 tttgtagaag atgaaggttg ctaccgaatc aatgaatatg gtgattttat ttgccagagt
     4021 tgccacgaga tagaatttcc aaccgaaaga agttggtttg aatacatgct acaagattat
     4081 gagatggaaa cagaaaatgg tgaagtagtt ttttgtacca aagaggaatt gagtaagaga
     4141 agcgatgatg aaattgaaga ttggtgtgtg tggttagtag ggacagatga ttgcgttgca
     4201 tattacacaa cagcagaata ggagtgtgaa tcagcatgga aggaatggat tttatagatc
     4261 atgaagattt gattgatttt ggttatacat ggaaaggaat ggtaggtatt agcagaagtc
     4321 ttgctaatgc cttttacgaa agaaattatg cagtctatgt cctttatgat gacaatacag
     4381 aaagccttgt tgatgaagag tataaattgg atttagagaa tgttttatat gggatagaaa
     4441 aagaagattt agcaaaatat attttttcat ggttgggtca ataaacgaaa gagggtagca
     4501 acatgacaaa attcaaaagt aaaggtatgc aatggtgttc ttattattat gttgggaatg
     4561 atgaagagct tttgagaaaa gccaaacaaa aaggttatcc agataagatg ttaaaacaga
     4621 ttggctatta catgccagaa gagagaatag aaaaagaatc ttgtaaggtt ctttttgata
     4681 gtgaacagct attagttttg ttaactcaaa ataaattgat ttgtcaattt gaaaataatt
     4741 aatatggggt gaagaaccat gaatgagaat aagatggtat taagtgaata cgcaagattg
     4801 agaaccatta attggttgaa tggtatgcaa agtagtggcg cttttgatgg atttgatgag
     4861 gagaatatgg actttgaaag tttatcgaat gaggatatta gatattattt tatcgaatgg
     4921 tattttacag aaccagatga agaggtggaa gatgaaccag aattaatttt agaacgtcaa
     4981 agaattttat tcagtgaaca agatagtaag aggttgtatg gtttatatga cttacaactg
     5041 aaaggattaa aagcgcagta tgttgctgat agttacgatg atatggtaga acagggggca
     5101 gatagggttg tcacttattc agaagatgat gaagaggaat gggaaaacta ttcagatgat
     5161 gagatatgct ttaatgatgg gtatatgcct attgaaatca ccgaagaaga atatgataaa
     5221 gtgtcggata gcgatgagat tggtatgtgt acggttgtaa gtggattgga tatagatgtc
     5281 caaagagtag aggttgcata ccagaagatg aaagaacgta taaaaagtaa agtagaatct
     5341 tgatttgatt ggattgtttg caaagtagct aattaagagt ataatataat cataaatgta
     5401 gttaatggat ataataatga gtatattata ctcaaaagag gtgaacgaaa tgaaggtaaa
     5461 aaatccaaaa gaaaaaagca gaattcaagt cggaatagac aaggatttaa aagaaaacgc
     5521 agaaatgata ttggaagagc tagggttaaa tccaacaaca gcaatcacaa tcctttataa
     5581 gcaagtggtc gctagaggtg agtttccagt agagataaag ctaagcgaag aggaaaagca
     5641 aggaatcaga ttgcaacagc taactatgga tatgccagtt gatgttctag atacagatga
     5701 aaagttagaa gagtggttca atgaagccta atgagatagt aacggtctat gttgcattta
     5761 cagataaaaa tggtggtaaa agaagaccta ttttggtggt tagtgataaa gaagatagag
     5821 tagaattttt tggaatcaca agtcaatatg agaataaatc agaccaaata aagagagtgt
     5881 attttcctat tgcagaatgg gaaaaagtag gtctaaagaa gcaatcatgg attgacgtag
     5941 ggtctttaaa ggctattccc aaaagccgag agaacatatc atttaaaaag gttggtatgc
     6001 tatcagctat tgatacgaaa aagctaaata ggtttatcga gagattacaa agaaaagtct
     6061 aacaaggctt ttcttttttt gtttatatgt atattaagat atacgtt
//
LOCUS       DAYYAG010000143         6035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000143 DAYYAG010000000
VERSION     DAYYAG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.146"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..1073
                     /locus_tag="ACHARQ_002573"
     CDS             45..1073
                     /locus_tag="ACHARQ_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410958.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1099..1539)
                     /locus_tag="ACHARQ_002574"
     CDS             complement(1099..1539)
                     /locus_tag="ACHARQ_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8410959.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1699..2205
                     /locus_tag="ACHARQ_002575"
     CDS             1699..2205
                     /locus_tag="ACHARQ_002575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8410960.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2373..4286
                     /locus_tag="ACHARQ_002576"
     CDS             2373..4286
                     /locus_tag="ACHARQ_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8410961.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4291..5292
                     /locus_tag="ACHARQ_002577"
     CDS             4291..5292
                     /locus_tag="ACHARQ_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8410962.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5494..5700)
                     /locus_tag="ACHARQ_002578"
     CDS             complement(5494..5700)
                     /locus_tag="ACHARQ_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8410963.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2230 a          925 c         1201 g         1679 t
ORIGIN      
        1 cgatagaagg agaagaatac cgtatccgat acttttttca cttgatgtat tggcagtcat
       61 tcgacgaaaa tcattctgat atattatcaa gtataaaaaa taaacgtcaa ctattagaaa
      121 aatttgcgaa acatgcgcca tatacgcgta gtatagatag aaagaaatgg atcagcctac
      181 taagaatttc gttgcaacga atgaaacaag gccactcgat tacgtatgtc ccagaagaga
      241 tgaacaaatt tgtgcatcca ttgataacaa aagaagagtt tacgcattac atactagagc
      301 ctttttttga agcaaacttc tggtcgatga atcacatcac tccaacagag caagcttacc
      361 tttattttat gtttggtgta atgaacacct atttggaaga agatttgttg gatactccag
      421 ataaagtaca agaaagttgg ggctggattg aatcgtctat tgtagaaaat tttgaaaaaa
      481 acttcaagat ttgttttaca acttcagaaa aaaattatct atatgtcaat ttggcaatga
      541 tccatttgta ttcaagggtt tttgggacaa aaaagaaggt ggatgctttt ggaaaatcag
      601 cagaagacag agattttcag catatatttc cagtgatgta tccaatcatg caacagtttt
      661 ttagtggggt agctcgcaga attccagagc ttaatactta ttatgaaaag aacagacggt
      721 tgatttttca atactgtatg ttagtcaggg atatttttat aaaacacgaa caacctgtca
      781 tattctatat tcaatcaaag tatggtaaaa cacaggaatt atgggcaaag aaaaggatat
      841 tatctatgac tgaacgccca ataaaatata gtgcatccgc tgatcagtta cctgatattg
      901 tcattagcga ttatcctatc gacaaaaagg catatgatca aacgaataca cagatatttt
      961 actggaatgg acaacctaca aaaaatgatt gggcgcgtct gaaagctgaa atcataaaaa
     1021 tcaggaatga gaaaaataga tccatattgg aaaaattgga tttcgttaat taagaaaagc
     1081 agattgttta tgccaaaatt aagacattgc aaaaagatta ttttctttaa aatcgacaag
     1141 atgaccagaa agcaagagtt cgttgagctc ttgctttctt ttagttttag aaaggaacat
     1201 cctgatttta tcttcgctaa atccagtcaa aagttttgca gattggtcgt agataatcgg
     1261 ttttttcaat aaagaagggg atgtacgaat ctcaatatat agttcactca aactgagttc
     1321 atctattctg tgaacaagat ttttataact aatagaatga gtagagagca tttcggtcac
     1381 gccgttgttt gtcaatttta gtaagtggat aaattcattt ttggttagtg gttgacgaat
     1441 taagttctgt tcgataaacg gtatatcatg gacgataagc caacgttttg cgcgtcttga
     1501 agacgagcag ccagaacctg tatacaattt aatcatcata cattcctcca atagctaagt
     1561 atttatgaaa taataacatg aagaaatagt cgaaaagtgc catttagagg tgtaaaataa
     1621 gaaaatgttg tcacgtataa tggaactagc tagataagaa atactattaa atcaagaaaa
     1681 aagaaagaag gcacaagtat gtcaaacaca gccgacgcaa tagtagtgat cgatttacaa
     1741 aaaggggtat gttttgattc ttcgccgata cacgaattag aaacagtgac agcattagtg
     1801 aataaaagaa tcaaaacata cgaaacgatt gggaaaccag ttattttcgt tcaacacgaa
     1861 gatgctcaat tagtgccaca aacaaaacca tgggagctat tgtcggaatt atctattcct
     1921 gagcacagtt attttgtgag aaaaacccat gcgaattcat tttataagac taatttgagt
     1981 gttttattaa ctgaattgga ctgtcattcg atcgaattat gtggggcaca agcagagtat
     2041 tgtatagata cgactgtgaa gtttgctcat ggattaggat accagcttat gatgtgtaga
     2101 ggagcaacga caacttatga taatcctttt atgaaagcaa aggaaacaat cgctttttac
     2161 gagaaaattt gggataaacg atttctgaca tttttggagc aataagttgt ttcttcgttt
     2221 aaaaaaagca gaatggatac tggtttatcc attctgcttt attacgtaaa atgagaaaat
     2281 aatcctgttc tatttttatg ggataaacca tttatttttt gtataaaaaa tgtttgcgtt
     2341 tacatataat tctcctagaa acataaaaag gagtgaatag cttgagaaca aaacaagtat
     2401 taaagggagc gttggtcatc ttatttgctg taagtacaag cgtcccgcaa tggacagtat
     2461 atggagagag catttcttcc gaatcggaat ctcgggaggt agaaaccaca gaaactactg
     2521 atgctggaaa tgaaacgaaa tcgagtactt cacaaacaga aacaaatact gaaacaacgg
     2581 ttgagacatc tgttactagc acagatcaag aaacgaccgc taccacagat aaccaacaaa
     2641 cagaagaagc aacagctaca aaaatgtcaa acgacgaatc ctcttctgct aaagtaagtg
     2701 acaaagagaa tgggacagaa aatgatgata tcggtaaagg tgaaaatttc aatggtaaag
     2761 aatatttgaa ttacttcggt aaacagatga cagaaatact taatggacca acagaagaca
     2821 tcaagtcaaa attaatagag ttattagtca ctgagcttgc tgttaatgat tatggtgtag
     2881 ataaagagat tatcaaacat ctgattgcta cttatctatt gtacgatatc gcttttgcaa
     2941 aaaacagtgt gttgatgtcg atgcttggag gtacagatga tgggacaacg cctacagaaa
     3001 acacaggctt ctatccaaat acaaaacaac ttcatcaaac tgtactaaac aaacaagatg
     3061 gcaaaatgat ggatgtatat gcatattatg tcgatcaagg ttcagataaa acagtcatga
     3121 tccatggcgg atttcgaggg aattggaata acgggatcgt tacagaagaa tacaatgatt
     3181 tttataaggc cgggtataat ttattatttg ttgattccag agcgactggt aatagcggag
     3241 gcgattatgt gacctatgga caatatgagt cagatgacgt cctttactgg ataaatcaag
     3301 aagttcgaga gcggccatct caaaagattt tactttatgg tggttcgatg ggagcagcta
     3361 cgatgatgtc tgtattagcg aaagatattc ctgtcaatgt caaaggaatt attgaaaatt
     3421 gcggatttgc atcaattgat gaacaactac gttttacgta cagccagaca gtagcccccg
     3481 cactaccgga tgccataaaa aatcagttag acatcattgg ggatcaagag catgaagatt
     3541 tatttatggg ccttttgaaa caatactatt ttgatcagga gatgcatttg gatacaaaag
     3601 ctgctctacc tacgattgga atgtcagatt cattgcctaa attgattatc catggaacag
     3661 cagatgatgt agtacctgtg tctaacgcgc aaaaattata tgagttatca ggagggtata
     3721 aagatctttt attagtagaa ggagcagggc atggaaaggc acaggaagtt gaccatgctg
     3781 cctacacgaa acatgtaacg gactttttga aagtagtgtt ccatgatcag ataaacgtaa
     3841 agtatgtgga tgaaaacaat aaaagtctac tcaaagacca ggacgaaatc cagctttacg
     3901 gagcttatgg agaaaattat atgacagagc aaaagacatt cgaaggatac gaattagcaa
     3961 atgttgaagg accgactgaa gggattttta atgagacgat accaactatt attttctatt
     4021 acaaaaaaat cccagtagct ccaccaaaaa agcaagatcc gacagagaat gcagacaata
     4081 agggaaagaa tgaacctcga gaggatcaat caagtgatga tacgcaaccg attcagatga
     4141 cagtgaaagt acaaaaaaag acagatgcaa aaaaaagaat gttgccgaaa actggagaaa
     4201 agaaacattc gcttctcatt tattcaggtg gatttttatc tgttagcacg attatcacat
     4261 ggatttggcg aagaaagaga gggtaaatga atgaaatggt tcaagcgaat agtgattgca
     4321 gtaccagtta tcatcgtagt tattttagtc actgtatttt tgattaatca aatcacacca
     4381 aagccgatta gcttgcttgt tagaaaacaa tttgacacaa gtgaaaaaga acaagtatat
     4441 gcccgaccag atgatttcca gcaaaaaatc gaacaaatcg atataaaaaa agaaatatcc
     4501 tatccttcaa agtacaaaaa taataatatg gatatctaca caccgaaagt ggaaaagaaa
     4561 aaattaccga ttcttttctg gatgcatgga ggcgcatatg taggagggga taaaaatgat
     4621 tgtcgtgatt atttggaata tctatgttca gacacgcagc aaatcatcgt aaatatagat
     4681 tatgaacgat caccggaagc aaaacatcct acaccagtta tccaattgaa cgaagccatt
     4741 caagcagcta aaaaagagat aaaagatcaa gcagattggt cgaatatttc gattggtgga
     4801 aattcggcag gtgcacaaat tagtggagaa tatttgttag cacttcaaaa tgaagaaatt
     4861 aaaaaagctg atcatcttga tccgactttg gagaacaagc agattacgaa gtttatttct
     4921 ttgtctggtc tgttggatcc ttctgcactt actcaagtaa gtgataaaat ctctagcttt
     4981 ctttatgaaa aatgtggatg ggcttacttt gataataagg actttgaaca atcagagaag
     5041 gtcaaaagtt tagccttggt cagacatgcg gatcgttgga cgcaaaacac attcttaacg
     5101 gatggaaata caaatacgtt tacgaaacaa atggaagaag taacttctgt atttgagaaa
     5161 gcgggggtga aagtcacaaa agtagattat gcggagaaaa atgctgtttt gaaccatgaa
     5221 tatcagtttg atttcagcaa taaacaagcg caagaaacat atcaaaagtt agtaatgttt
     5281 ttcaaggagt aaagtcttat gatgcaaaag aggttgtaat ctgtcactta gctccaagtc
     5341 acaaggagca atttcaataa acaagctttt catggtttat gaatattgta acttgtgact
     5401 gaggagttag agatggcaag gggcaccttt tattcgaaat ttgggaatat cacacgatta
     5461 ttgtcacgat ctcttttacg taaattttat aagtcagatt tgttgttttg atcgtttaaa
     5521 aaaagcaaca aatccgaagc ctgcggaggc aagaagggac aaaaagccga gtggtaagca
     5581 aaagaatgct ggttccacta atattccgtt gttattgata taaggctggg aacaaagatg
     5641 aatagcaaat aaaatcaatg cactagcgaa aaaaagcaaa gaaatatgga tagctttcat
     5701 aagcagcacc tcctttttta taattgtatg ataaatagat taagaatcta taaatgtgac
     5761 aagtttgtta ggatgtgaat aaaatgatca tctagcgaac ttgaaacaaa gatggctaat
     5821 gataagcaat taatgattca gcaatcagat aggtacaaag tttaccatca tacgtctaga
     5881 ccaataatag aaaatatggg aatataatag aaaagattta ggataccccc aaaagaattt
     5941 ccccaaaaat tcgatgtatc ctaaactttt ttattttaca taacaatcat atcaactgtg
     6001 aaataaaatt acaaatgata acctaaaatg aggcc
//
LOCUS       DAYYAG010000144         5968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000144 DAYYAG010000000
VERSION     DAYYAG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.147"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1667
                     /locus_tag="ACHARQ_002579"
     CDS             30..1667
                     /locus_tag="ACHARQ_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410964.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1812..2552)
                     /gene="truA"
                     /locus_tag="ACHARQ_002580"
     CDS             complement(1812..2552)
                     /gene="truA"
                     /locus_tag="ACHARQ_002580"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8410965.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2743..3378)
                     /locus_tag="ACHARQ_002581"
     CDS             complement(2743..3378)
                     /locus_tag="ACHARQ_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8410966.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3525..4745)
                     /gene="sstT"
                     /locus_tag="ACHARQ_002582"
     CDS             complement(3525..4745)
                     /gene="sstT"
                     /locus_tag="ACHARQ_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8410967.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4964..5962
                     /locus_tag="ACHARQ_002583"
     CDS             4964..5962
                     /locus_tag="ACHARQ_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410968.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
BASE COUNT         1934 a         1117 c          898 g         2019 t
ORIGIN      
        1 tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata
       61 tcatttttat gttatttcta agttttgctt acgtcatccc tctattcatg tctaagggaa
      121 tctatcatag tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata
      181 atgtatggag ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata
      241 ttttttatcc atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc
      301 ttgttttaag ttataatatc tactattttt ttatcacttt tttaacaatg gtcgtttctt
      361 atttttctat gaaacaaata aaaaacaatc gttatatttc gctgttattt tcgattattt
      421 atacattttc cgcctatcgt gccattgaca tattccatcg tgcttcattg ggagaagctg
      481 tcgctttaac atttttgcct ttgatactaa tggggtgtta cgaaatttat attagagatt
      541 accaaaaatg gtactggttg tctattggca tgacgctagt agtgtatact catttacttt
      601 ctgtcgctat ggtttctgta tttattggag gaacgttgtt tctttctttt tatttttggg
      661 accaaaaaat cgctagactc ttaagtcttt taaaagctac cgtcctcacc ttttttttaa
      721 gtgcgggatt cttaattcct tttattcaac aatctagagc acaagaattg aaagtcccac
      781 tgggaaaaga attaagtggt atggctccta gtgatatgct gacacatatt ttaaataata
      841 actataacaa ctatactatt ggattattct tatttctcgg attgattggt gcttttattt
      901 ttataaaaaa attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt
      961 tttgttcaac caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac
     1021 aatttgtctg gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag
     1081 tgatctatta tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc
     1141 ttgcactaat attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat
     1201 taacacttat agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt
     1261 atgcaaacaa agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag
     1321 gtaaccagga aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag
     1381 ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag
     1441 tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat
     1501 taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa
     1561 ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat cgatttactt
     1621 tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag
     1681 aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat
     1741 ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta
     1801 tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
     1861 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
     1921 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
     1981 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
     2041 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
     2101 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
     2161 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
     2221 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
     2281 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
     2341 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
     2401 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
     2461 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
     2521 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
     2581 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga
     2641 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
     2701 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
     2761 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
     2821 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
     2881 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
     2941 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
     3001 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
     3061 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
     3121 tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag gcaatgacag
     3181 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
     3241 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
     3301 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
     3361 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
     3421 tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
     3481 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
     3541 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
     3601 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
     3661 ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca
     3721 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
     3781 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc
     3841 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
     3901 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
     3961 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
     4021 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
     4081 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
     4141 aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc
     4201 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
     4261 atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc
     4321 aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga
     4381 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
     4441 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct
     4501 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
     4561 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
     4621 gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg
     4681 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
     4741 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
     4801 atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa
     4861 ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag
     4921 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
     4981 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
     5041 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
     5101 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
     5161 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
     5221 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
     5281 caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct
     5341 aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag
     5401 tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact
     5461 attattttta acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc
     5521 attacagaat ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt
     5581 gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt
     5641 gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa
     5701 tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt
     5761 ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa
     5821 ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta
     5881 tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa
     5941 tatcgggcag ttttttattt gatattta
//
LOCUS       DAYYAG010000145         5946 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000145 DAYYAG010000000
VERSION     DAYYAG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.148"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..451)
                     /locus_tag="ACHARQ_002584"
     CDS             complement(<1..451)
                     /locus_tag="ACHARQ_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8410969.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            675..1466
                     /locus_tag="ACHARQ_002585"
     CDS             675..1466
                     /locus_tag="ACHARQ_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8410970.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1432..2385
                     /locus_tag="ACHARQ_002586"
     CDS             1432..2385
                     /locus_tag="ACHARQ_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFM8410971.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2783..3757)
                     /locus_tag="ACHARQ_002587"
     CDS             complement(2783..3757)
                     /locus_tag="ACHARQ_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8410972.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3872..4306
                     /locus_tag="ACHARQ_002588"
     CDS             3872..4306
                     /locus_tag="ACHARQ_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8410973.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4431..5453)
                     /locus_tag="ACHARQ_002589"
     CDS             complement(4431..5453)
                     /locus_tag="ACHARQ_002589"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM8410974.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5845..>5946
                     /locus_tag="ACHARQ_002590"
     CDS             5845..>5946
                     /locus_tag="ACHARQ_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFM8410975.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIR"
BASE COUNT         1871 a         1121 c         1018 g         1936 t
ORIGIN      
        1 aatttgtaat aagttcgtat atgttttgcg gtgtttcttg tttcttgtaa ttttccacga
       61 atgtttcatc catgaataaa ttcataaacg cttgaagtaa tcctacttgt tcttttggtt
      121 ctttgattcc taaaaaaaat agattttcta cagtcatttc ctgattatcc cccatttgat
      181 aaaaataaat tgggttcttc agctgcataa caaaaacaaa tggcttttcg acatattcac
      241 attccgaatg tggcagcgcg atatttaaat acttagtaat caatccagta ggaaattctc
      301 tttctcttgc aatcagtcca ttcaaataat tctcattgac gtaccccatt ttttctaatt
      361 gttgtgcagc gattaaaaaa gcttcttctt tattttttac ttctaccgct aattgaatca
      421 aactaggaac gaacatctct ttatatttca ttccaacctc ctcctttttg attaagtttg
      481 ttaatttatt gttctactaa attcatcttt gttttacaag aacattatat ttctgttagt
      541 ttgttttgtc aacgctttca aaagaaattt attttgttaa atttgatcta cttgaaaata
      601 aaatcgttta tctatataat taagaagaac aaagtcgaac aaaaattgac ttttatgtta
      661 aaggagtaat tcatatgctg aaaaaagaaa gacaagaaca aatcattaca ttattagaga
      721 aagattttta tttaactaat tcagaaatcg ctaacaaatt aggcgtatca gaaatgacca
      781 tacgccgaga tgtcactgaa ttagcaaaaa aaaataaagt aaaaaagcta tatggtggcg
      841 ttgaacgaaa ggaaccttat aatcgagaac tatcgactca agaaaagatt gatacgaatg
      901 tagccgaaaa aaaatatatt ggtcaagtca tgaacagtat gattcccgat aattctacta
      961 tttatgtcgg tgctggaaca acgatcctat atgcacttcc tttcattcaa aagaaaaatc
     1021 tatttgtaat tacaaacagt ttgattgctt tcaactattt aaagaatcat acaagttatc
     1081 ggattttact tactggtggc gaattttctc ctattacaga agagttcatt ggggaagttg
     1141 cagaaagatc ttttgaaagt ttgaatgtcg atatctcttt cgctgcaaca aacggcatat
     1201 ttgataacaa tgtgacaacc gctcaattta ttgagggaaa tattcagaac gcagcgctca
     1261 atgcagcaaa aatcaaatgc attgttgcag attattcaaa attaaataaa tcagacgtct
     1321 atacttttag aaaattaacc gattttgatt atctaataac cgataatcat ttatcagaac
     1381 aggatttcaa tcattattca aaatacttaa caatattaaa ggagcccaaa aatgatactg
     1441 acaatcacga tgaacccatc cattgattcc atttatttta caaaacactt tgaattaggt
     1501 acgatgacac gctttgattc tccggtacgc tatgtaggcg gcaagggaat caattgcgga
     1561 cgagcttctg ctctactagg ttcatcagtt attttaacag ggtttttggg cggttatttt
     1621 ggcagtatga ttcaagatat gctgaaaaat gaaaatttgt ttgatttgca ttttttacct
     1681 attgcagaag aatcaagagg tgccgtcacg atcatgcatg atgatgaaac gcagacagag
     1741 ctagtggaag ctggtcctga aatagcagat actactatca aaaaattaat gaatgatatc
     1801 aaactaatca gtaaaaataa ttacctttca gtcattacaa taaatggttc tgttaacaat
     1861 ccagatccct ttttgtatgc taaattatta caatatatca aagaacaaat agatccttcc
     1921 acctatatat taatggatat atctggacaa catctccgtg aagtcttagc aagtaagtat
     1981 aaaccgatgt tcatcaaacc taatatccat gagttttcag atatcatcca tcaaaaaatt
     2041 actcaaaaaa gcgacataaa acaccatcta aaaacaaatc cactatttaa agatatcgat
     2101 gttatcatga tttcttgcgg ctcgtatggt gcagtagtca aagtaaaaga agaattttgg
     2161 aatgttgaga tccctaagat tcctatcatt aatacaacag gctctgggga tgccactgtt
     2221 ggaggttttg ctcattcatt agaacagaat tatcctttaa aagaatgtgt aaggtacgca
     2281 atggcttgcg gcatgtcgaa tgcacagcaa tcagaagtcg ggaaagttat tccagctgat
     2341 gtaaaaaatt tagcaaaaag cattaaaatc acagaaatga aataaatagt acaaagtaaa
     2401 taattgttta tatgaaatga gacacaaaga gactgtgaca aaaaacgtgt gtaccgaccc
     2461 ccaaaagtta gattttttgg tctaactttt gggagtcgat acattgtcac agtccttttt
     2521 tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca
     2581 tgagaagcta ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacgac
     2641 ctcttggtat aaacggtgtt caaaagcttg catctacgct aatgagtcca accaagtcaa
     2701 aaatgaggag cttttttggc tgtccttatt acttgatgca gggcagtttt ttcataatct
     2761 cttttagatt aaatcctttt ggttacacaa ggttctcttc aaagaatgtt gccatttttt
     2821 caaatggaat gatgtctgtc tggtcgtata gatctgtatg ggatgctcct ggaatgatca
     2881 tgagttcctt gttgtcacct tttagttctt tgaaagcgtc ctccccaaag taacgtgaat
     2941 gagctttttc cccgtgaatg actaagactg catgacggat ttctggaata taatgcaata
     3001 gttttgcatt cataagtgac aaccctgatg tcctattcca accatcattg gagtttaaag
     3061 aacggacatg atatccgcgg tctgttttat aatacgcatg atagtctttg acaaattgag
     3121 gagcatcttc aggcaacgga tcaaccacac ctccagcacg tgcgtagtcc ccttgtttga
     3181 aatcaatgat gcgttgtgca tttaattctt cacgcatttc atatcgggca ttttcatcca
     3241 taacatcgaa gtagccattt gctgttactc gagtcatgtc atacattgtg gaaacaactg
     3301 ttgctttgac ccgcgtatcg atagctgctg cattcaatgc catacctccc catccacaaa
     3361 taccaatgat tccgattctc tcagggttta catttttttg tacagataaa aagtctactg
     3421 ccgcttggaa atcttccgta ttgatatcag gcgaagcaac atatcgaggt actcctccgc
     3481 tttctcctgt aaacgatgga tcaaacgcaa tcgttaaaaa gcctctttca gccatcgtct
     3541 gcgcatatag accagacgat tgttccttca ctgcaccaaa tggcccgctc acagcgattg
     3601 ccggcaattt cttttcactt acttcttttg gttcatataa atcagcgact aatgtgatac
     3661 caaaacgatt cgggaagctc acctttcgat gattgacttg attgcttttt ggaaatactt
     3721 tgtcccattc ttgtgtgatg tttaattctt tattcatatt ctagactctc ctttgatcaa
     3781 aattgacatt tgtttttgaa tgtattatta tgataaaact taaacttgac tttaggtcaa
     3841 ggtaaaaatt ttattttagg aggatttttt tatgtattcc attggacaag tgtctgaaat
     3901 gtttggattg cctgtttcta ctttacgtta ttacgacaaa gagggattat ttcctgatat
     3961 ccagcgtgtt tctggtatta gaaaattttc agagaatgag ttagaggctt taagagtcat
     4021 tgactgtttg aaaaaatctg gtttagaaat aaaagacatt aagcaattca tggaatggag
     4081 caaagaaggc agtaagacat ttgaggttcg taaagaacta tttgaaaaac aaaaagaagt
     4141 agtagaaaaa gaaatagcaa aattagaaag agtactcgct atgctaaact acaaaagctg
     4201 gtactatgaa gaagcaataa aagccggcaa tgaagaagcc gtattgacaa tgattcctga
     4261 tgatttaccc caacatgtaa aagaagctta tgtacattct cattgaacaa acggataact
     4321 cactctcctt ctctatgaac ttcatgaaaa catgaataaa cacctctaac tgattgttag
     4381 aagtgtttat tttttcactc aagtatcctg cacttacaaa aatatccttt ttaccgccat
     4441 cctaattctg gagcgatatc ttttacgatt gtttcaagca agtgcatgtt atattctacc
     4501 cccaaagtat taggaatcgt gacaagtact gtgtctgctt ctttgattgc ttcatcttca
     4561 gctagctcct tgattaattg atcaggttca ccagcaaaag tttttccaaa aatcgttggg
     4621 tttcggtgat aagcaagata gccgacagca tcttgttgcg gactaccgtc ttgtccaaat
     4681 aagcgtcgat ccaaatcagt ggtgattggc tgaatcgagc gagtaactaa tgttcttggt
     4741 tcaaaatcat gacctgcttg cttccacgct tctttatacg cacgtaattg gtttgcttgt
     4801 tggatatgga atggctcgtg ggattcgtaa ttgactaatg tagatgattg cagattcatt
     4861 ccatgttgtg ctgcccagat tgccgtttgc tgcgaaccag ctccccacca gattctttgt
     4921 cgtaatcctt ctgaataagg ttctatccgt aattttcctg atccttgtgg gaacattggt
     4981 tgaggatttg gttcttcaaa tggttttcct tcaagtaatt ccaagaattc aagggtgcgt
     5041 tcccgtgtta cctctttgat ttcttcttca ctgtaatcat aaccaaagta tttccagcca
     5101 tctagaactt gttcaggtga accacgcccc actcctagtt gcactcttcc ttgcgtgatg
     5161 atatctgtca atccggcatt ttctgccata taataagggt tttcatatcg catatcaata
     5221 agtcctgtac cgatttcaat cttacttgtc tttgcgccga ttgccgctag caaaggaaac
     5281 ggcgacccaa tctgaggggc aaaatgatgt acgcggaaaa acgctccgtc cactccgatt
     5341 ttttctgctt ccaccgctaa atcaatcgat tgcaaataag catcttgagc tgatttcaca
     5401 agcgatcctt gctccatcca atgtccaaag cttaaaaagc cgattttttt caaggtaatg
     5461 acctccttta aacctacaaa aatggaaggt tttttatttt taatatgaac tgattctact
     5521 actttttttc ttaaaagacg aataattgtt ctcgcctcac aatcaagaaa tgtatcggct
     5581 tactacaccc ctactttctt catcatctgc cgtgtcttaa tctcttttaa cagtctgtta
     5641 catcgttgat ttttgactca aaatgattgt ttctaacatg tatttatggt aaaaaagaca
     5701 agattagttc tttgtacaca tcgatttata cgctgaacaa aaagaaatat ataaactaag
     5761 aaacccattg ttttttcgct ccatcacata gagtttactt tagataaaca gtgacattta
     5821 ctaatccata acggaggaaa tcaaatgaaa aaaatctatt tcttatgtac gggcaattca
     5881 tgccgcagtc aaatagcaga aggctacgcg cgtaaatact tgtctccttc aatgtttgag
     5941 ataaga
//
LOCUS       DAYYAG010000146         5779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000146 DAYYAG010000000
VERSION     DAYYAG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.149"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHARQ_002591"
     CDS             <1..813
                     /locus_tag="ACHARQ_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8410976.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHARQ_002592"
     CDS             786..1229
                     /locus_tag="ACHARQ_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410977.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="ACHARQ_002593"
     CDS             2363..3331
                     /locus_tag="ACHARQ_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8410978.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="ACHARQ_002594"
     CDS             3341..3829
                     /locus_tag="ACHARQ_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8410979.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="ACHARQ_002595"
     CDS             4125..4406
                     /locus_tag="ACHARQ_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410980.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="ACHARQ_002596"
     CDS             4408..4824
                     /locus_tag="ACHARQ_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410981.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="ACHARQ_002597"
     CDS             5438..>5779
                     /locus_tag="ACHARQ_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8410982.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2155 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcccttggt
     5281 acaggcgtta cttggtagta tacacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DAYYAG010000147         5744 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000147 DAYYAG010000000
VERSION     DAYYAG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.150"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1479)
                     /locus_tag="ACHARQ_002598"
     CDS             complement(82..1479)
                     /locus_tag="ACHARQ_002598"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8410983.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(1511..2482)
                     /locus_tag="ACHARQ_002599"
     CDS             complement(1511..2482)
                     /locus_tag="ACHARQ_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8410984.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2856..3281
                     /locus_tag="ACHARQ_002600"
     CDS             2856..3281
                     /locus_tag="ACHARQ_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410985.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(3769..4470)
                     /locus_tag="ACHARQ_002601"
     CDS             complement(3769..4470)
                     /locus_tag="ACHARQ_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8410986.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(4486..4926)
                     /locus_tag="ACHARQ_002602"
     CDS             complement(4486..4926)
                     /locus_tag="ACHARQ_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8410987.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(4926..5501)
                     /locus_tag="ACHARQ_002603"
     CDS             complement(4926..5501)
                     /locus_tag="ACHARQ_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8410988.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         1655 a         1192 c          913 g         1984 t
ORIGIN      
        1 aagatcgtaa ataattgaac ttctttttca caaaatggta agaatggaga atttcacgca
       61 tttgctctca actattcttt cttaagcctc aaatccgttt ttatctgata cgtctttgaa
      121 ccaatgaccc gattttttga ttgttctttt tccatcttgt gctaagtcta ctgaaataaa
      181 gccatatcga ttcttatatg cattggtcca cgaccagtta tccatacatg tccacatatg
      241 atagcctaaa caattggcac cttcttgaat ggcttgatgg acatatttca gatgagattg
      301 gacaaattcg atccgataat catcgtggat catgccatct tctttgataa atcgtgcttc
      361 gttttctaca cccataccgt tttcagaaat aaagcagtcg atatttccat aatgatctcg
      421 tacattgatc aaaatatcat agattccttg ttcatagatc tcccatccgc gataaggatt
      481 cattttacga ccaggcattt cataattgtc gaaataatcc tcaggcattg gaccgttttt
      541 agtatcgatt ggtgtttctt ttgcttttac ccggcgtggc tggtaataat tgatacctaa
      601 aagatctact gtattttctt tgataaccgt caaatcctct gcatggatca caggcatgag
      661 atcaagttct tttactatct caatcaattc acttggaaac tcgcctttta cagctggatc
      721 aaggaatgaa cgattgaaga acgcatctgc tatttgcgct gctttaacat cttcagggtt
      781 tgattcgtct cttggatagc ttggtgtcag attcaagatg ataccaataa caccaccttg
      841 ctccatttca tggtaagatt tgatggctaa cgctgaagcc aacgcttcat gatagccaac
      901 ttggatcgct tttttcatat ctgactcttc tggataatgc catcgatata aataacctgc
      961 ttcgactgga acgattggtt cattatgagt aaaccatttt tttactttcg aaccaaacaa
     1021 ttcaaaacaa cttttggcat atttttcata ggcttcaacc gtttctcggt tcaaccatcc
     1081 gcctttttct tgcagataca tcggcatatc gaaatgatac agattcacaa agggttcgat
     1141 accgttttca atcaactcat caaaataacg atgatagaaa cgaacggctt cttcgttgat
     1201 tgttccagat tcatcaggta tcaaacggct ccattggata gatgttcgaa atgaattatg
     1261 cccagtctgt ttcatcaact gtacatcctc ttgatacttc gtataaactt gcgaggtttt
     1321 ttcaggtcct acttgatcaa agaacttttg cggttctttt ttgtaccaat aatcaaaaat
     1381 gttctctcct tttccatcgc ctgaaatcct tccttctgtt tgtgggccac ttgctgcaga
     1441 accccaccaa aaatttgctg gaaattgata ttttttcatc aaagcacctc tttttctttt
     1501 ttagttcgtt ttagttgatt ccatgtatga tattttccat cacaatcact ttttcatttt
     1561 cttcctcttc ttttttgatt cgttttaaca gctgttctgc tgctcgttcc ccgattttat
     1621 ctgttggtgg cgtgatcgtg attttcggaa ggatactcag atcgttttta tctaaataat
     1681 catagtgact gaacgcaata tccttcccga ttctgacacc ttcttgattg aaacattcga
     1741 tggcaccaat cgacatattg taattgctga caaaaatagc agttgcccct ttttctaata
     1801 atgcttttgc cccgtaatag ccgctttttc tagaatagtc tcctgtaaag acctctaatt
     1861 ggatcgatgg atcattttgg accgcttctt tggctcctgc taaacgttct cttgctacgt
     1921 agtctgtttg agtggcagca ataaatccga tttttttatg tccttgttca ccatgatgag
     1981 cgatcacttt tgctacactt tctcgatcat tcactaatat tgtatcaata cctgctggtc
     2041 cttctggaca attcaatgcg ataactggaa tactcatttt tgtgagcatt tcaaatccag
     2101 accactcttc ttcggataag aagacaatca gcccatcgat cacatgcttc atcaggtatt
     2161 caattttttg ttcaattttt tctggctgat cggcaaaccc gcttaataat aagctatacc
     2221 cattttcttc agctactctt tcgatccctg agacaacttc cgacccaaaa cggctggaaa
     2281 tcccgtttat caaaagtcca atagagaagc ttcgattatt cttcaaccct ttagcaatga
     2341 tgttttcttt atagtccaac gctgaaatcg cttctgctat ttttttcatg ttcttttcct
     2401 tcagttgctg accattcaag taacgggaaa ctgttcccac tgaaacacca gccatcttag
     2461 ccacatcacg gatcgatgcc atacattctc actcctgcca gttacatttg atttctcttt
     2521 cttgttgttc gattatgatg accgcattct ttgcatagtg ttgcttgtca tcaatatcca
     2581 tataactaaa gtcttcagta gagcaacttc ctctcactat taattatact taaacaagta
     2641 tttgaaccgg ttcattttta atatagcatt tttttaaaag attgcaacac ttttttaaaa
     2701 cgctttctga aagtggcgga ttttttgcct ttttaaaatt ttattctaaa atttactcta
     2761 tttgtgggtg ttttgtgcgt ttttctcttc catccgtttg aagttttaca ttccaatggt
     2821 ataatttctt tatcctatca aatgggggaa gtattttgaa gaaattaatc gtactattta
     2881 ttttgtctgc gggacttttg acattgactg gctgtgattt gtttggcggc tctgaccaat
     2941 cctcggacga aacaaccgat tcttctgtct taaaagcgga aaaagcagat accaccaaca
     3001 gtgtatatac gctaaaagta cgtaaagata tggatttatc cgcgggacag ttgacattta
     3061 cagataaaaa aatggaatgg acacgcacgt atcagacaca aggaactgat tcttctgaat
     3121 catctgaaga aaaaacagtg ctgacagata tcaagatcac taccaaagat gacgaatata
     3181 ttatttcagg aaaagaaaat aaaaaagaaa caacagtcat atttacaaaa atcggtaatt
     3241 accgtttgaa ggatgaggat ggcaacatct acagtctata gtgccgtctg ttggatcttc
     3301 ttttgtttct ccccttcttt tctgctatag taaagaagca agatgattgt aaaggagacg
     3361 aaatggatga tcgatagtcc tgattattgt acatgtaata acaacaaaaa tatccataca
     3421 gaatttaatg accaaggaag cttgagcata tgcgacaact gtcacaaact agtcaaagat
     3481 tcatttaaaa catttgaatc ttatcctgtt caaaattatg taaaaagtag aagaagataa
     3541 acaaatccaa gcaaaactca ctatggctga tttttatgaa cttcaaactc agcttcttcc
     3601 tttgatcaag cttcatcagt aaaacaaaca gtggatcatt tcagtttaca aatcaaagag
     3661 taaagattta tttatttcaa atatttgaat ataaaataac caaacgatag aaaggcttat
     3721 tctacatcac gtagtaattt ttctttcccg tcgtttggtt ttttattttt agcctaattc
     3781 ttgacctttt gcatgctggt tatccgtaaa atattttcca gcaggctttc cttccaattc
     3841 agctaatttt tcttggatct gacggatcac cttttttgca tcttctggat tttctacaaa
     3901 atcgaattta tccccatcca cttgtatttt aggacataca tcaaaatctt cataccattg
     3961 attatagcgg gtgttcaacg tttcataata ggtatataac gtctcatcgc tttctagctg
     4021 ttcatagtca cgcccacgtt ttttgatacg ttctagcatc gtgtccaaag aaactttgat
     4081 atggaccatc aaatcaggcc gtttttttgg agcaacatca tctaactcgt ttagcatcgt
     4141 atctaataag ctatcgtact gctgtacttc gatatccgtt acccgaccta aatcagcgtt
     4201 tagatggaac aacaggctat cttcatagat ggatcggtct agcacattgt catcgttcac
     4261 taaagcattt ttcattgcta aaaatcgctt gttcaaaaag aagatttgca ataaaaaagc
     4321 gtactgctca ggatttgaat aaaagagcgg caatacttcg ttatcttcga cattttcata
     4381 aaatggttta gaatcaatct cttttgcaag catatccgtc aaactgctct tacctgcacc
     4441 aatcgttcct gaaatcaaaa tcactgacat acaacccttc ctcttctata agtactcgcc
     4501 tacgtaagtt tcaacaggca atgtttcaaa atcatattca cgtacagctt ggtctgattt
     4561 taaatacgta tggagacttt gtgtaatcat caagttcgta ggtaccgtct cttcttgtac
     4621 aatgatcatc ggttttttca acatagttgc cacacctaat tcgtaagccg ttcctggatc
     4681 gattgtttga tgctcaaagt caaggatagc aacaacaaat tctgcatttg tcagttcttc
     4741 catatctttt tcgtatactt cctgcgccca tccagcgctg aatagttcat aattgctttc
     4801 ttggtgatga cgtggagagt agaaacttgt gactgttttg ttttcctcca acgctttttc
     4861 gatacgactt actcgatcga tttgctcctc tgaaaaaaat ggtcctgcta aatatatttg
     4921 actcattatt ttgcctcctt caataataac tgatttttaa tagatactgc tcgtaattca
     4981 ttgaccagtt ccaatggatt gttggaaaag attacttgat ccaaccattt tttatccata
     5041 aaatcctctt ttgcaaaagt ttctaataat tgttgcaagc cgctgtaata accgccaata
     5101 ttcaatattc cgattggctt gtcgggcgtc agaccgattg ctatccaaga aagaagttgt
     5161 gagaattcct ccaatgtacc tagacctcca gggaaaacga ggacagcatc tcccttttca
     5221 aataataata cctttctttc atcgatcgtt tctgtaggaa taaatgtagt aacatctttt
     5281 ccaggcaatt cagtttcaaa aagcccattc ggatagatac ctgtgacttt tccgttattt
     5341 tccagtactg ttttactaat cactcccata atcccggagt atgaaccacc gtaaaccagt
     5401 tcgatttctt cttgtgccat gtattttcct aacgtttgcg caataaattt gtaactttct
     5461 ttattaccaa aacgggaacc gcaaaaaaca gtaagtttca taaaaaaaca ctccctaact
     5521 caaaagtcta tcttcattct acacgtgttt ttttaaaata aaagactaga ttttgtagtt
     5581 ttttagtggt atataggata gacgccacta tatatagtat ttagaaaaaa atcatttttc
     5641 agccatttta tagataaaac aaaaataaat aacgtgtttt catcgatatg aaatgactta
     5701 cttatagaag atactgggat ttcgtcctat atgccaataa cgtg
//
LOCUS       DAYYAG010000148         5696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000148 DAYYAG010000000
VERSION     DAYYAG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.151"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHARQ_002604"
     CDS             complement(<1..300)
                     /locus_tag="ACHARQ_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8410989.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHARQ_002605"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHARQ_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHARQ_002606"
     CDS             complement(1014..1304)
                     /locus_tag="ACHARQ_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410990.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHARQ_002607"
     CDS             1706..1942
                     /locus_tag="ACHARQ_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8410991.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHARQ_002608"
     CDS             1949..3901
                     /locus_tag="ACHARQ_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8410992.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHARQ_002609"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHARQ_002609"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8410993.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACHARQ_002610"
     CDS             complement(4797..5489)
                     /locus_tag="ACHARQ_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8410994.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1861 a          986 c          911 g         1938 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaaca
//
LOCUS       DAYYAG010000149         5643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000149 DAYYAG010000000
VERSION     DAYYAG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.152"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      177..343
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            491..1048
                     /locus_tag="ACHARQ_002611"
                     /pseudo
     CDS             491..1048
                     /locus_tag="ACHARQ_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1106..3145
                     /locus_tag="ACHARQ_002612"
     CDS             1106..3145
                     /locus_tag="ACHARQ_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8410995.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3254..4405)
                     /locus_tag="ACHARQ_002613"
     CDS             complement(3254..4405)
                     /locus_tag="ACHARQ_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8410996.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4559..5089)
                     /locus_tag="ACHARQ_002614"
     CDS             complement(4559..5089)
                     /locus_tag="ACHARQ_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8410997.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5188..5538)
                     /locus_tag="ACHARQ_002615"
     CDS             complement(5188..5538)
                     /locus_tag="ACHARQ_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8410998.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2048 a         1032 c         1053 g         1510 t
ORIGIN      
        1 aaaaaattat caaagaatcc tggtgcaact gaaagaattt gcaacgggat tctttcaact
       61 tatttgttat ttttttctaa aaaatattaa agattgttaa atttcactat ttcatttcaa
      121 attccattgc ctaatcatta ggaaaagtct atcatgaact cgtaaataga atatagtaat
      181 aaccttgtta ggtgaggctc ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc
      241 gccaatgggt aaaacagaaa ctgttggatt aaggcttttt ccaatagcta agaatgtttt
      301 ctttacgttg tatagtgcta aaactcggac gatgagatga tgctaggtca gaattcttgt
      361 tcgaaaaggt ttctgacctt ttctttatat ttttgtgact gacttatttg atgagacaaa
      421 ctcgctcctc acctgacaag tggattgctg tgtgaggagg agatgcggtg aaaaaagatc
      481 gattggtgga ggtcgctcgg attcacttct ggttgcacaa tacaaccaga aaacacaaca
      541 agcaaaactg atctctatca tgagagattc atatgtcgat attccaggtt acggaatgaa
      601 taagataaat gcagcttact cttatggggg agttgattta ttaaatcaaa cattaaagga
      661 aaatttcaaa tttgaagctc cctattatgc aagtatcaca tttcaagatt ttatcgattg
      721 cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc tagatttaga
      781 tggcgtatat ataaacaaag gagagcaagt gatggatggt aatactttac tccagtatgc
      841 tcgatttcgg gaagacaaag aaggagactt cgggaggatc agaaggcagc agcaagtgat
      901 caaagctatc tctcagcagc tgaaagacgt aacttcaata ttcaagctac ccaaagcggt
      961 tgggaaatta cttggtagta tccaaacgaa ccttccagaa agtgtgcttc tggattgtgg
     1021 tatggatttc ctcaaagaca tatgttagaa taaacaggta taaatagtga aataaaggaa
     1081 tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac aagcacggca aaaatcgaac
     1141 aggcgcttat attgccggcg cagtgatctt aatagcagct gcgggtggcg gttattttta
     1201 ctaccagcac tatcaagaaa cccaagcagt agaagctgga gaaaagacag ttgagcaatt
     1261 tgtccaagct ttgaacaagg gagattataa caaagctgca gaaatgacat caaaaaaggc
     1321 ggcaaataaa agtgcattat ctgaaaaaga gatcttagat caataccaga atatatacgg
     1381 tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa aagacgattc
     1441 tacttacagt ttttcatata aagcaaagat gaatacctca ttaggtgaat tgaaagatct
     1501 ttcttataaa ggaacattag acagaaatga tggtcaaacc acgatcaact ggcaacccaa
     1561 cttggttttt ccagaaatgg aaggaaatga caaagtaagc ctgaccacac aagaagcagc
     1621 aagagggaat attatagatc gaaatggtga accattagca acaacaggca aactaaaaca
     1681 attaggagtc gttccaagta aacttggaga tggaggcgaa aaaacagcca atatcaaagc
     1741 gattgcttcc tccttcgact taacagaaga tgctatcaat caggcgattt cacaaagctg
     1801 ggtacaaccc gattactttg tcccattgaa aatcattgat ggagcaacgc cagaacttcc
     1861 agctggagct accattcaag aagtagacgg cagatattat cctttgggtg aagcagctgc
     1921 tcagctgatt ggttacgtgg gagatatcac agcagaagat attgataaga atccagaatt
     1981 aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg gcttttgata aggatcttcg
     2041 tgggacaaca ggtgagaaat taagcattac agatgcagac ggtgtagaga aaaaagttct
     2101 gatcgaacat gaagttcaaa atggcaaaga tatcaaattg acaatcgatg cgaaggcaca
     2161 aaaaacagct tttgacagtc taggaggaaa agctggatca actgttgcga caacgccaaa
     2221 aaccggtgat cttcttgcgc ttgctagctc tccaagctat gatccaaaca aaatgacaaa
     2281 cgggatctca caagaagatt acaaagctta tgaagaaaat cctgaacatc cattcatcag
     2341 ccgatttgcg acaggttatg ctcctggatc tacgtttaaa ataattacag cagcaatcgg
     2401 tctcgacaac ggcactatcg atccaaatga agtgttgacg atcaacgggc ttaaatggca
     2461 aaaagacagt tcttggggat cttttaaaac aacgcgtgtc agtagtgatg tatcacaagt
     2521 agacttaaaa actgctttga tctattccga taatatatat gcggcacaag aaacgttgaa
     2581 aatgggtgag aaaaaatttc gtacaggctt agataaattc atttttggtg aagaccttga
     2641 tttgccaatt agtatgaatc cagcacaaat ttctaatgaa gatagcttta actcagatat
     2701 cttgctagct gatactggat atggacaggg cgaacttcta attactccta tccagcaagc
     2761 agcaatgtat tctgttttta ccaacaatgg cacacttgtc tatcctaaat tgattgcaga
     2821 taaagagaca aaagataaga agaatgtcat cagcgaaaca gcagtacaaa cgatcgtgcc
     2881 agatctgaga gaagttgtgc aagatgtaaa tggtacagca cattctcttt ctgctttagg
     2941 gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg atgtaaaggg
     3001 gcaagagaac agtttcttgt ttgctttcaa tcttgataac caaggttata tgatggtcag
     3061 catgttggaa aataaagatg atgatgattc agcaaccaaa cgagcatcgg aactattgca
     3121 atacctcaac caaaattatc aataaaaaat tgctttttaa gtacagtaaa aggactgtgg
     3181 cgttatcgtc acagtccttt tttcaaataa acggttttca cgatgtctct ttcacaactt
     3241 ctttttgatt tactcacact tcataaactt tttttgtaaa atatttgaga acaagtggag
     3301 aaataagtgt acttaataaa atcacgataa ctaaaggtga atagtactgt ggttcgatca
     3361 attgactttg ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc gaaatcatac
     3421 cagcaccgac catcaatgaa ctatgttgag aaaaaccggc aatctgagca ccaaaataac
     3481 ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat agaaataaag agtagttgtt
     3541 cactaaagtc agaaaaatca acttctaatc ctacactgac aaaaaatact ggtataaata
     3601 ctgcatatcc taaagcctct acattgtggt atacttcttc tttaaccttt gtctgactga
     3661 cagctatccc agcaaaaaaa gcaccaatga cagagctaag cccaacaaga tccgctacgt
     3721 aagacattcc taaacaaata actagcgata cgatgataat agaagaattt gcgtacactt
     3781 tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac taataagaaa ataaaaacaa
     3841 aataaattag ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa gcttcccccg
     3901 ttagaaaaga taggctgaaa cttagaacta aaacgactaa aatgtcatct accacagaag
     3961 cacccaaaat tgttgatcct tcttttgtat tcactacgtt tagttctttt aatacttcta
     4021 ctgaaatact gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct tcatttcccg
     4081 aaacttggaa tgctttgcct gtcaaccagc caaaaacaac cgggaagatg atcccaagca
     4141 aagcaacgaa cataccaggt cggaaatatt ttttcaaaag agataaatca ctttccagac
     4201 cagctaaaaa cattaataag aacacaccaa tctccgaaaa gtcatggact agaatatcag
     4261 gtttgaccca attcaatcca gcttggccta gcaaaacacc aacaagtaat tgtccaatca
     4321 ctgctgggat acctaatctc cgcgagatat gtgagccaat cgttgttgaa atcaatatca
     4381 ggcataaaat accaataaat tccattctca tcattccttt gtataaaata tagcccgaac
     4441 ttagcataac atacattatt gaaaaaatag gaaaatatac tcacaaaaaa accaacaatc
     4501 caaaagattt ttttggattg ttggttttta tacggtctat tttaataact aaaaatgatt
     4561 aacgtatagt catttttctt attctgatga aattgataat ctcctggatg cccatgaaaa
     4621 tcaaaataaa gccgaatatt tggaatagaa gcatcaaact tttgaatgga ttgaacaata
     4681 agacgaatcc gccaatcaac aacagaatac tactataaac actccaactt ttagcttttg
     4741 tattcatgct gaagaacaac tggaacaagc tgttgatgat aacagctaat ccaagaagaa
     4801 tcggcaggat agaaacgatt gcaggtgcaa aagcatagac gatcagagct aagatcagga
     4861 aaaaaagacc actgaacaga ccgatactcc aagaacctgt tttcctttta aaacggtaag
     4921 cctcaaaaag attcaacaac ccaagcaaaa caagataggc ggtgatcaga taaccgataa
     4981 aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc agaaataata taaacagcag
     5041 ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt ctcaaacata taagaaaacc
     5101 tcctttgact tattttaacg tgaactagtc attttaccaa attaaatgtt acaaaatgag
     5161 cgaaggagaa gaatatctct ggaaaaatca acttctgagg atcttttcca tttttttgcc
     5221 ttttgctagc tcatcgatca ttttatctaa ataacgaatt tctttcatca agttatcctc
     5281 gatgtcttct actcgtacac cacaaacaac acctgtaatc aaggaacgtg aaggattggg
     5341 gacaggggct tccgcaaaaa attgttcgaa agtcacttgt ttttgtagtt gatattctag
     5401 ttctgtttgg gaataacccg tcagccaaca aatgatttca tctacttcct cttttatacg
     5461 atcttttctc tccgcttttg ctacatacat aggataaacg cttgcaaaac ttgtcgtaaa
     5521 aatacgattt tgcgccataa tcagtcacct ttcttttttt caaattattt actacttata
     5581 gtatataaga aaaagagaag aaaaagcgat tattcaaaaa aacaaaaagg attgtgacaa
     5641 aat
//
LOCUS       DAYYAG010000150         5589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000150 DAYYAG010000000
VERSION     DAYYAG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.153"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACHARQ_002616"
     CDS             <1..377
                     /locus_tag="ACHARQ_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM8410999.1"
                     /translation="KDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEI
                     VPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKA
                     DYVTVSNDKDGVSQAFERILTI"
     gene            658..1878
                     /locus_tag="ACHARQ_002617"
     CDS             658..1878
                     /locus_tag="ACHARQ_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8411000.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1875..4595
                     /locus_tag="ACHARQ_002618"
     CDS             1875..4595
                     /locus_tag="ACHARQ_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8411001.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4579..5523
                     /locus_tag="ACHARQ_002619"
     CDS             4579..5523
                     /locus_tag="ACHARQ_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8411002.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2076 a          899 c         1101 g         1513 t
ORIGIN      
        1 aaaaggatgc tatcagcatt tacaaatttt ttatttttat tcctaatcgt caattgaaag
       61 ctaatatatt taaccatttg aaaaagatgc taaatgtgtc tgttactgaa tcggcacctg
      121 taaatattga aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat cgtttggaat
      181 caatctacaa tttaaagaaa cctttgagaa tcgcgatcgg agatagttta aatgacttat
      241 ctatgtttga aagtgcagat attagttttg caatgggaaa tagtcatcaa gaaatcatga
      301 aaaaagctga ttatgtcact gtttcaaatg ataaagacgg agtatctcaa gcatttgaaa
      361 gaattcttac catataaact aattaaggta gtatagaaca tagaattcta aatacggaaa
      421 aatggagcat aaattccagt acaaatagta cttcttcagt gtcaaacgaa gcgaatataa
      481 catagatatt taggattgtg acaaaaatca tgtcacaatc tttttttcga ataaacttat
      541 tttttgaagt cggccattta tcgtaatttc tttgcataat acgaacaaat tttcggttat
      601 tgatgaaaaa aaacattgaa aatggtatga taaaagaaaa aaaggaggaa atttttaatg
      661 gtgaaattta tccatgcggc agatttacat atggatcggt catttgaagg actgacaact
      721 cttgataaaa ctgtacagga aaaactattg aagacgaatc tgaccgtttt atcaaatatc
      781 attgaacagg caataataaa taacgttgat tttgttttac ttgcaggaga taattttcat
      841 caaaatcgtc cttcattaaa aatacagaaa catttttcag aacagatgaa acggttagaa
      901 aagaaccata tacctgtatt tataatattc ggaaatcatg atttctatca aaaagagcgg
      961 tattggttct cctttcctaa aaatgtccat ttatttacat ccgaaacggt agagacaaaa
     1021 aaaataacga taaaaagtgg agaaactgtc tctctcagtg gatttagtta tcgtcaaccg
     1081 tggattcaaa aagacaaagt gatggaattt ccttctagag aactcacaga ttaccatatt
     1141 gggttgtacc acggggaacc tggcgtctct caaaaaggaa actatgctcc tttccaaccc
     1201 tcaaagatgc aagaaaaggg atacgattat tgggctttag ggcatatcca tgtgccaacg
     1261 gtcttaaatg aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca tactaaaaag
     1321 gaacaagctt ctacatctat tctactagtt gaattaagta aaggaagttg ccaaatacat
     1381 ccgattaaag tagcagaggt ctattggaag acaaaggaaa tatcattgag gaaagcaaga
     1441 acgacaaaag aggtcattgt tcacattcgt cagcagcttt caaaagtaga ggatggattt
     1501 agtttaattg agataaaatt aaaagattat gaccatctca atacagatgt tttggaacgg
     1561 atacaatcag gagagctatt agactatttg ttagaggaat tctcagatag gataaatgat
     1621 ttctttattt ggcgtatttc tctcatagag aatacttttt atacgaagac cccacttgct
     1681 gcttctgcta aattgatgga acagttattt caatactatc agacacctca agatttccaa
     1741 caaattttaa atgaagtata tagtcatcaa gaagcggcta gaatattgag cgaactgccg
     1801 gaatatcaag aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga ttttttattt
     1861 gaggaggatc aggaatgaag attttaagga tcgagattgc tgcttttgga aaatggcgtc
     1921 aaaaaagttt tgacttttat tcaggcaatc agctgatcta tgggggaaac gaagcaggaa
     1981 aatcaacgat ttatcaattt attcaagcaa tattatttgg tttcccttca aaaggccgta
     2041 aaaaaaagga ttatacacca aaagacggct cagcctatgg cggcaaaata tggcttaaac
     2101 atcctgtata tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc ggaaaatcga
     2161 aggtgtggct gggggatcaa gtgggaagtg atgagttact agaaaaactg atcgcacctt
     2221 tgacgaaaga gttattccag caggtattta ctttccagca agagcagttg atggaactag
     2281 atcatttgag agaaaaagag ctacatgatg cgctgatttc tttaggcatc acgggcagtt
     2341 ctcaagtatt ccaaaagcgg caagaatatt atcagaaagc acaacaatta ttcaagaaaa
     2401 aaggccaaaa attactaatc aatcaaaaat taaatgaatg gaaagagctg caagaaaaaa
     2461 ttcatcaaaa acaagaacag gaagcttatt ttggcaagtt gatccaacaa gaacaggaat
     2521 acgagtcgca acagcagcag ttattggcac aattaaaaga ggcgaacaaa caattttcat
     2581 tagccagaca gcaagaactt aatttttctt tatatgaaga atggcaatct ctcaaaaaaa
     2641 acagcactac aggtcttcct gatgagcaac ttcttttaga tctggagtct ttctccaaac
     2701 ggtatcagca aacaaatgag cgtttggaag aattagaagc aacactggaa aagcatagcg
     2761 ggatggatca gcagtcagcc agatattatt tttatttaga gcaggagcag gaactaaaag
     2821 atctccagca acttcaattt acagcggaac aattaaacga agaatggcaa agattacaaa
     2881 ttgagactag agaaacaaat tcagaattga agattttgga acaaaggtgg cattggcaga
     2941 aaaatccgtt accggaagaa tttttatatg aagaagaatg gaagcatctt ctggctgaag
     3001 aaagaaggat tgctgatcta gaaagccaga tttctgtgcg tctccaaata gctaaagaac
     3061 agcaagagat gattgaagca gaagtgaatg acttggaaga gacctatcca gcattattac
     3121 aaaatcagtc agagatagca ccagtctctt catctaaacc gtatatgctg atggctggcg
     3181 gagcaataat cattgcaggt atatttgtac caatgccctt gaaagtgatc cttttactaa
     3241 ttggtgcagg catgattggc tatttcttct acaaaaggca accaaaaatt aaaactaaaa
     3301 atgatttctc tgagataaaa ggaatgtggc aagaaaaact aggacaactt gatctagcct
     3361 tagctcaatt agctgaagca gaagaaaaac aaaagagaac tggagaagaa aaacagattc
     3421 ttcagcaaga agtatatcgt tttgcggatc atcggcactt aggaaagatg gatacgctca
     3481 aactcatgaa agaatatgga caggaagtca tagattatca gaaattatat ggcgtatttg
     3541 agcggaaaca taaaagagaa caggaaatac aagaaaaact acgacaaatc gaccaaaaat
     3601 ttgagtttgt taaagaatgg ttaccgatac aagataagac aatttcagaa aaaatggtat
     3661 tgctttcccg ttttatacaa gagatggaac agatgaaatt tgccagaagc tatcagcaaa
     3721 atactttatt gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta tggatacaag
     3781 aatacagcga acaattgact caagcaggct tatcttatcc ttcggaagta cctgtctttt
     3841 tacaaaaagc caaacaagcc gaacagcaga tgaaaagata caatgaatta tcggaaatgc
     3901 ttactccact atttcctgaa gagctgacaa aagagaaatt atctgtaaga ctgcgccaac
     3961 tgaaagaaaa gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa aatcggcaac
     4021 gactccaact tcaagtagaa gagttgcaaa gagacggcac attggacgaa ctttatcaac
     4081 aagctagccg gcaaaaagca aaattacagg aattactgga ggaatgggga gcacttgaaa
     4141 tcgccgggac attcttaggt gatctttcaa cagagatgag tgaaagacaa ttaccccagt
     4201 tgttaagagc agcaggtgat tatttaggta ttttaacaaa aggaaattat cagaagatat
     4261 tactgaaaaa tgatacactg actttaacag acggatatac agaatttccc atctatgagt
     4321 tatctacagg tacgaaagat cagctgatta tggcgatgag atttgcttac ctctcattag
     4381 aagctaaccg ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat tatgatcatc
     4441 agcgaaagta tcaattagct gaacttttag ctaaatttgg agaaaagtat caaataattt
     4501 gtttttcttc tgataaagaa atggtaagct attaccatga gatgaaccaa atcgtaaaac
     4561 aattagaagg agcaaataat gaaaaaaatt cgtgatttag taatcgatga gcagtttgaa
     4621 caatttgtcc tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa aaaatttatt
     4681 gcttttactt tccaagatac ttcaggtact atcgatggta aatactggga tgcttcggag
     4741 gaggaaatca ataaatttac agccggcaca gtcgtttttc taaacggtaa aagagaggtt
     4801 tatcaaggaa accctcaagt aaagattctt catatgcgct taacaagggt agatgagcca
     4861 ggagagccta gtttatacat ggaaagagcc cctttgaaga aagaagaaat ggaagaagaa
     4921 atcaatcaat tgttgtttga gatcaccaat gcgcattgga atcgtattgt tcgctatcta
     4981 cttaataaat atcaaaaaca attttttgag tttccggcag caaaaagaaa ccatcatgca
     5041 tttgccagtg gattagctta tcatactttg acaatgcttc acttagggaa atcaatcgcc
     5101 gatgaatatc ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt gcatgattta
     5161 ggaaaggtga ttgaactttc cggtcctatg tcgacagaat atacgcttgc aggaaatttg
     5221 atcggtcatc tggtattgat tgacgaagaa ataactaaag cctgtcttgc gttgaaaatc
     5281 agcgaagctg acgaagatgt tctcgtatta cgtcatatgg ttttagcaca ccatggctta
     5341 ttagaatatg gctcacctgt acgtccaaga ataatggaag cagaaatatt acatcaaatc
     5401 gacaatatcg atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac agaaccagga
     5461 aaatacaccg atcggatttt cggtatggac aaccgaagtt tctatgtacc aaaagattta
     5521 tagcaaaaat ttattttttt cattcaaaac aaaaaaagcg agacataagt cttcgctttt
     5581 tttgttttc
//
LOCUS       DAYYAG010000151         5539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000151 DAYYAG010000000
VERSION     DAYYAG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.154"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1557)
                     /locus_tag="ACHARQ_002620"
     CDS             complement(52..1557)
                     /locus_tag="ACHARQ_002620"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8411003.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1649..2239)
                     /locus_tag="ACHARQ_002621"
     CDS             complement(1649..2239)
                     /locus_tag="ACHARQ_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8411004.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2418..4064
                     /locus_tag="ACHARQ_002622"
     CDS             2418..4064
                     /locus_tag="ACHARQ_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8411005.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4113..4832
                     /locus_tag="ACHARQ_002623"
     CDS             4113..4832
                     /locus_tag="ACHARQ_002623"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8411006.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5311..>5539
                     /locus_tag="ACHARQ_002624"
     CDS             5311..>5539
                     /locus_tag="ACHARQ_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8411007.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFS"
BASE COUNT         1665 a         1007 c         1122 g         1745 t
ORIGIN      
        1 cacagtccct ttttttctta gaaatgggaa accactagta tataaatacc attattcttc
       61 aatcaaccgc ttcacgattg cataatcccc ttcataaggt tcatctacac cattgatttt
      121 ttgataggga tccacgcctt ttcccgcaat aacgactgaa tcttgcggag cgctttctgc
      181 taaagcgcgt cgaatcgctt caggacgatc catttcataa tgaacgatca aatctggatt
      241 agtaattgct tcattgattt cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc
      301 tgccgtcaaa aaggcgatgt cagctgtttc tgatagcact tttccaaaat catgtcgacg
      361 ggagattgct ttgtttcctg ggctgcctaa tacaacaatt acacggccat ctggatgttc
      421 atttttggca aattccagca atgttttcaa gcttagataa ttatgtgcat agtccacata
      481 gacatgcgct ccattggatt ggatcaattg atccattcgg cctggtacac gagcttcttt
      541 taatccgtct tgtgcatctt gactggaggc acctgttagt gctgcggcaa tgatcgcact
      601 ggctgcattt tcttggttga atcctccagc cagcaaaata tcataagcag tatcgcagat
      661 agctaagcga tcttcatgag aagataaagt aaaaccgtga gtacctttat ctgaacggat
      721 cacttgataa ttgctttctt ccgaacgacc ataagtgatc gttggaatct ggaatagatc
      781 ggctgtttcc ctaagtaaat caaaatagtc actttcatga tttaaaacaa ctgtttttga
      841 attcaagatc aattgtcgct tgcaatagaa atagtcatca aatgtcggat gttcgatcgg
      901 actgatatgg tcaggcgaga tattcaagaa gattccgaca tctaaagtca agccgtatac
      961 gcgttgtgtt ttgtaagctt gtgaagaaac ttccatcact agatgagtca taccattttc
     1021 aactgcttct gacatcatac gatataaatc taatgactca ggcgttgtca aatgggattt
     1081 gaaatatgtt ttgccatcta acgttgtatt catagtagaa agcaatgctg tttttttatt
     1141 tgttgtgtga tccaagattg cttttgtaaa gtacgcagca gttgttttac cttttgttcc
     1201 agtgaatccg atcactttca atttattttg cggatagtcg taaaaagcca tagcaagaag
     1261 cgccattgct ttacggatat ccgttacgat gattcctact gctgaatttt cgtatggttg
     1321 ttctgataca taatattgaa taccctgttc gattgctgaa tccaaatatt cttctttgaa
     1381 attcattcct ttacagaaaa acaaagtaga agcatcagct tttcttgaat cataacttaa
     1441 ctgaagaaat tcagcttttt ctggtacatc aagatgccag cctcttgttg aaacaaattc
     1501 ttttaataga ttttctttta atagtaaatg acggatgtct tctaaagaga agctcattga
     1561 ttgatccctc gctttattca atttcttggt ttattatagc acactctttt taatatatcg
     1621 tttgtttccg ctatgacaaa atccagcttt attcaaattt tcttgacaca ggggacaagc
     1681 ggatgagtat tttcataaac aatgaactga ctgtaataga taggagaacg aatagtgtct
     1741 ccaacaatcc aaaaacataa taattcaaaa agacgataag actattggta agtaaaagtc
     1801 cttgcggcat tggaagccga aaaaatttgt ggaaaataat cggcggtatc gttataccgc
     1861 catttgaggc cccgactgtg tacaatgtgt aaaccccaag actgaacaaa agacttcctg
     1921 aaatcagtga aaagaaatgt gaggagatca tttccacttt tggaacaatc tctaaaacaa
     1981 caggaaaaag cagacttccc gctacagtct taaaaaatac tgattttcct aataggacag
     2041 cggctagaag cagcatcata agattgatgc tccaaacagt aactgagcgt tggatagaaa
     2101 aggccgtttc aactaaatgt ccgatggcgc ccactccggc ggaagctata tcgtgaggga
     2161 gcaagaacca attcaatcca actgtgataa agaaaagacc taaaaatata acgggtagtt
     2221 tttttatttc ttgtgtcatt gatatctcct ataaatgtta gccataaaag gctgtatatt
     2281 tctttacttt accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt
     2341 atataaaatc gattgccagt ttctgtttta ccaattaaaa tgatagtaat gttttgtaga
     2401 aagttggaat ctgaccgatg gataatcaaa gaaaaccagc attagatcaa ttaacaccgc
     2461 aagaaaaaat ggcacgaggc tctgcttgga tgaccgccag taatatcatt tcaagattgc
     2521 taggcgctgt ctatatcatt ccctggtatg cttggatggg cgaaaatgct aaggctgcca
     2581 atggattatt caatatggga tacaatatat atgcattgtt tctactgatc tctacagcag
     2641 gaatacctgc tgcgattgca aaacaaactg cacgctataa ttcattgaat gaatatggca
     2701 cttcacgacg attatttatc cgtgcattac agatgatggg cgggctaggc ctgttgtttg
     2761 ctttgttcat gtacatcgcc tctccttggc tagctcatgc ttctggtggc ggagaagaac
     2821 tgattccaac gatgcgttct ttgagtatcg ctgtattggt ttttccttgt atgagtgtga
     2881 tacgagggta ttttcaaggc aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag
     2941 aacaggtggc acgtattttt tatatgctat tggctacatt catcatcatg aaagtcatgg
     3001 atggtgacta tgtgacggcg gtcacgcaat caactttcgc ggcatttatc ggtatgctag
     3061 caagtattct tgtccttttg tattttttga agaaacatca agcgtataca tcggcgttta
     3121 ttcactacag cgaaaacaaa ataaatattg caacgaagga attgttgtta gataccgtta
     3181 aagaagcgat tccgtttatt attgtgggtt caggggtaac gatcttcaaa ttagtcgatc
     3241 agttcacttt tatgaagatc atgtctgcat ccaccgagta ttccaatgca cagttactgg
     3301 atcttttttc gatttttagt gccaatccag ataagttgac gatggtagtt attgcattgg
     3361 caacatcgat cgcgtctact gggttaccgt tgattacaga agctgttacg ttgaaagatc
     3421 gagtaggtct ggcaaaattg acaagcggaa atttacagtt gttttcattt tttatgtttc
     3481 cggcgacatt tggtgttatg ttacttgcct atccgctaaa tacattattt tatacaccgg
     3541 attctctagg aagtaatgta ctgatccaag caagttttgt aggactcttc ctaggactat
     3601 atatgctagt ttccaacatg ctgcaaggaa tgtttgaaaa caaggcggca attaattatt
     3661 tggttattgg gtttctagtg aaactccttc ttcaataccc agccattcgt ttatttgaag
     3721 tatacggccc tttattggcg acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa
     3781 agagaatcca tgaagtagcc cgctttaaac caagttttgt ctggcgcagg acattgctga
     3841 tctttattct taccttgatt atgcttttgg cttcgtggat cacaaagatg atttttggtg
     3901 cttttttaaa cgaagatagc aaattccagt ctctattgtt gattcttgta gtagcaggta
     3961 taggggcttt agtgtatggt tatttagctt tgaaaatccg cttggcagaa agactactag
     4021 gaccaggaat agcgcgttta cgacataaat taaaaataaa ataagtgaat cagaagagct
     4081 agattgatgc tcttttgaaa tgggggaaac gcatgcgttt agataagttt ttagcagata
     4141 tgggacttgg cagccgcaaa gaagtcaaag gattgatcaa aaaaggaacg atcgaagtca
     4201 atggacaagt ggtgaaaagc gataaagcgc aagtaaatga attcgaagat gcggttactt
     4261 atttaggaga aatcattacg tatcaaaaag atttttacta cattttgcat aaacctgcag
     4321 gagtcatttc tgctactcag gataatcatg atcaaaccgt aatcgattta ttggaagacc
     4381 aggattatcg ggaagatctt tttccagtcg gccgattaga taaagacaca gagggcctct
     4441 tgattttaac gaatgacggg gtgtttgccc atcagttact atcacctaaa aaacatgtgg
     4501 agaaagaata tctagctgag gttagcggta taatgacaga agaagatatc cgtttatttg
     4561 cagaaggact gattattgat ggcgatgagc agacattgcc ggcaaaactc caaattgatc
     4621 aagtagatca agagacagaa acttcaatta tccgtctgat tttgcatgaa ggaaagtttc
     4681 accaagttaa gcgaatggtc aaagcagtag gaaaagaagt cttatattta aaaagaatcc
     4741 gtatgggaaa attttggctg cctaaagatt tgaataaagg gcaatatcga cccatgacca
     4801 aagaagagct agaacaagtg agagaaaaat aattgaatgg atagctaaaa gaataagcag
     4861 agtagttgta gcgccatctg aaaagcagac ttgtccgtct gactttcgga tggctttttt
     4921 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttgt tcggaataag
     4981 ggactgtgac aagacttttg ttacagtccc cattgttcct tgtgactcag agttagatag
     5041 taaggacacg atctccctaa tactcaggcc aaaacgctga attcacaacc tctgataaac
     5101 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     5161 tttcccgttt ttttctctaa tactcaggcc aaaacgctga tttcacaacc tctcctatac
     5221 tttgtttcat tttccctata ctttgtttat ttattctcat tattgaaagc gctataatta
     5281 cgttgcgaat gaatcgaaag gggtttttag atgaaaaaac gaaaggggtt caatcatttt
     5341 tttatgaatc tatggcgtta taaagcactt gtcctgatgg ctgtaccggg tatgctttgg
     5401 atgatttttt tcttttacat acctgtaatg gcgaatgtcg tagcattcaa aaattttcat
     5461 atttcagcag atgggttctt ggctagcttg agggaaagtc cttgggtggg ttgggaaaat
     5521 ttcaaatttt tattttctt
//
LOCUS       DAYYAG010000152         5481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000152 DAYYAG010000000
VERSION     DAYYAG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.155"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..262
                     /locus_tag="ACHARQ_002625"
     CDS             <1..262
                     /locus_tag="ACHARQ_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411008.1"
                     /translation="KLTDQEKISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKM
                     KRFNVILEIFAWLIPSFIWFFYIFIVESELIAKVSLGLLIPI"
     gene            347..547
                     /locus_tag="ACHARQ_002626"
     CDS             347..547
                     /locus_tag="ACHARQ_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411009.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            681..1907
                     /locus_tag="ACHARQ_002627"
     CDS             681..1907
                     /locus_tag="ACHARQ_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411010.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2504..2899
                     /locus_tag="ACHARQ_002628"
                     /pseudo
     CDS             2504..2899
                     /locus_tag="ACHARQ_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            2977..4016
                     /locus_tag="ACHARQ_002629"
     CDS             join(2977..3474,3477..4016)
                     /locus_tag="ACHARQ_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFM8411011.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            4029..4967
                     /locus_tag="ACHARQ_002630"
                     /pseudo
     CDS             4029..4967
                     /locus_tag="ACHARQ_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            4960..5307
                     /locus_tag="ACHARQ_002631"
     CDS             4960..5307
                     /locus_tag="ACHARQ_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411012.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         2026 a          803 c         1094 g         1558 t
ORIGIN      
        1 aaaactaaca gatcaggaaa aaatttctta tatgctaagt ttagataatc taggaactaa
       61 atatgatgaa tcagcaagta gcgataagct tttgattgaa ttagtatcaa gtcttagtag
      121 ttttggaaaa atgaaaagat ttaatgtgat attagaaatt tttgcttggt taatcccttc
      181 atttatttgg tttttttata ttttcatagt tgaatctgag ttaatagcca aggtctctct
      241 tggactgtta attccgatat aaataggttc agcaatttac tttaataact tgtatacacc
      301 tattcaaata aaaaagcagt tattacatga aggattaagt ggtaatatgt taaaaagaat
      361 tgcatgggat aaagataata aaacagatat tgagcttaaa gaatataaat acaattattt
      421 ctcgagatgt atagatagaa agttactgtt taaaaaagaa ggattagaat ttagttttga
      481 tgaagatgaa tttgaaaaat tagtgatgga ctggcgtaag aatttactgg atacggtaca
      541 attctaaaag caattgaggt cgaaattccg atacttgaat catgatctat attatcgtgt
      601 tatgagtgaa atttctaata aagataagat atttcatttg gacagtcatg tgaatatgag
      661 cttaacaatt aaacgtattt atgatctttt atatgtttat aaacattaat gaggtgctaa
      721 aattgacaac aataactatg acaaaaaaaa tagaagtata tgttgatgga tcacaagaag
      781 aaagggattc cttttttgat tttttttgga aaaatagtag agaatatgta aaagcgttga
      841 atctggtata taaacagcta atgtttgaag agttgataaa agatgcaatt aaagaaatgg
      901 atcaaggtta tgctgagaga gaagaaagta ttcaaaatcg gattgaaaag ttagcatata
      961 ctgatccaat agatcaaaaa aaacttgata aagcaaaagc tgaattatct aaattgagaa
     1021 atgaaaaaaa taaagaagcg agagaaatat taacgcaagc gatagggttg aagagtcaaa
     1081 cgagaacacg agatgtagca aaaaaattag actttgactt tggagatttt gttgattccg
     1141 caaatatgaa agctagtaaa gattttggca atgattttat cggaattgtg accggacaaa
     1201 gaacgcctag aatatataaa aatagaaaca aatatggatt attacctttt agaggtcgag
     1261 atattaaaat ccataaagat gataagtttt atatttattt acctaaaggc tttaggttta
     1321 aagtgaattt aggtagcaag ccaagaaaag caagagatgt tgcttcagtt ttacaacaca
     1381 ttatggatgg cgattacaaa ttaaatcaaa gtaatcttat ttttgatgga agaaaattat
     1441 tttttaatct aacaatttct tttgaaaaaa gctataatga aatggaattc aagaaaggta
     1501 atgaattgat cgtaaacttt gcagatttgt gtagcggtga atgtttaatt aatgataaat
     1561 ctgtaatgtc ttttggtgat agccaatata taagaaactt caaaagtaaa attttagcaa
     1621 tgaaatcaaa agaacaaagc cgaggagtct tttctaaggg cggtcatggt cgaaaaagaa
     1681 aattagatca agatcgatgg gaagcaataa agaaaagaga aagtaatttt gttaaaactt
     1741 ataataatca atgttctgca aaaatcgtta aaactgctat tcgcaataaa gctcaaatta
     1801 ttcgtatcat tgaaccagat aaggcttatg gcgaatgggc ttactatcaa ttgaaagaac
     1861 aactagtaaa caaaggaaaa agatatggga tagaagtcag aagttagtaa attaaacaat
     1921 cgttgatttg gcaacgaaat agtgagggaa aatccactca ctgaagggta aactccgtca
     1981 agttgtgtga ccagacggaa ggacgaatgt tgttgaggtt ttagaaccat ataaaacagc
     2041 atgtttctca aatttttagg ataagggaaa ttgctagcag tttgttttag aaccatataa
     2101 aacagcatgt ttctcaaatg agtactttaa attaattgat ggcgttcctg ctttagaacc
     2161 atataaaaca acatggattt aaaacaagaa gtaagtcagt tctcctttta ctttagtaag
     2221 gaaaaataaa gtattctcaa taaaacttaa aataagcaaa cctttgtttt aaaatgattg
     2281 tcaagttaaa aaaatctggg atcatataaa atagtatagt tctcaaatat gattagaaac
     2341 cctattaaag agcaatatta agccaactta cttaaatcgt ttaattaaga acaataatct
     2401 cttttagtca gacttttagt ctgacttttt tgtatttcca aaatttattc tttacaaaaa
     2461 cgaacatacg tttgtataat tacttgtgaa tgggggaata aaaatgtttg attacagtag
     2521 agaaccggtg aatgactatt ttttaatcga catgaagtct ttctatgcta gcgtggaatg
     2581 tattgaacga aatttagacc caatgactac accgttaatt gtcatgagta gatcggacaa
     2641 cactggttca ggtctgactc tagcttcttc acctactgca aagaaaaaat atcatattac
     2701 taatgttagt agagctactg atttacctta tccattacca aaagaacttc atattgttcc
     2761 accacgaatg aacttatata taaaaaaaaa tatggaagtt aatcagattt ttagaaagta
     2821 tgtcgcagat gaagatttat tgatttattc gattgatgaa tcaattttaa aagtgacaaa
     2881 atccttaaat ttatttacta tataaattga gttaaattgt attcgttttt cctcctatag
     2941 gcgtatacta aagaaaacac ttgaggagtg gggaacatgg aaacagaacg ccaaatccgt
     3001 gaattggaaa aagcgatgaa tacgacagac aaccgccggt tgtacgagcg tttcctggca
     3061 gtcaagttgg ttcttgaagg ccacacccgg aaagaagccg ggcacaccat cggccgcgac
     3121 gagcatactg tcagccacta tgtgaaaaac tactgccata atggaatcca aggtttagtc
     3181 tccaaaaaac aaagcggacg accttcacgg ctgacagatg aacaggaagc ggaactggtc
     3241 caaatcgtcg cctaccacac tccggaggaa gtaggtttca aatcccgtgc caattggaca
     3301 ttggcgattg tgtgtgacct gattctccgg gaatggagtt atggatatac gcaaaaaggc
     3361 gtggcggacc tccttcaccg gctaggacta agctggaccc gtccgacgta taccctcaaa
     3421 aaagcggatt ccaagaaaca acaggtattt ttggaagaaa ccttcccgag cctaaaaaaa
     3481 aattgctgaa tagcgatatt gaccacctcc tattcgagga cgaaaccatg atccgggatt
     3541 accaagccat tggctgcacc tggtttctcc gcggccacca gcgaaaaatc cccacttatg
     3601 gacaacacaa aggggtaaaa ttgataggca ccttggatta tgaaaccggt cggatcattt
     3661 gtacagaaga aggggcatac gacgcagcgg ccttcctccg ttttctggag cggacgttgc
     3721 gggaataccc caccggaaaa atcgtgatgg tattggataa tgcccttatc caccacgcca
     3781 agctgatcca gtcttttctt gaagaaaacg ctaggcgcct ggcactcatc ttcctccctc
     3841 cctacagccc tcagttgaac ctaatggaag gcgtttggaa gtggctcaag gaatcggtca
     3901 tcaacaatgt tttttttgac cacgtccaga aaatcaaaca gtcggtccgc ggtttcttag
     3961 ccgatgtaaa cgagcgtccc cttgaggtga ttgaccgact gtgcgtgcgg atgtaaagta
     4021 tttaattcaa cctatatagt tcaggaacaa gaagcgagag aagaagagaa ttagctaaaa
     4081 taatcaaaga tgagattcag aaagaaacag gtctaatagc aacagtcgga ataggagata
     4141 acccattatt agctaaactt gcactggaca atgaagcgaa gagtagtaaa gaatttatag
     4201 ctgagtggac ttatgagact gtacctgaaa aaatatggaa aataaacgag atgactgact
     4261 tttggggcat tggatcacgc atgaaaaaaa gattgaatca tatgggaatt atgagtatag
     4321 aagatttagc aaattggaat ccctacacaa ttaaaagtca gttaggtgta atcggactcc
     4381 agttatattt ccataccaat ggaattgatc gtacggatat tgctattcca ccagaaccaa
     4441 ccaaagaaaa gtcttatggg aattcacaag ttctaccaaa agattatacg aggagagaag
     4501 aagtagaact agtagtcaag gagatggcag agcaagtagc cattcgaatt agacaacaca
     4561 attgcaaaac tggttgtgta cgcttagcaa ttggtacgtc aattttggaa gaaaaatcag
     4621 gtttctcgca ccagatgaaa ataccaatta cagataatac gaaagaatta caaaattatt
     4681 gtcttttttt attcaataag ttttatgagg ggcaggaagt acgacatata ggagtaactt
     4741 atagcagatt agtatacaca gattccctac aattagattt atttagtgat ccaagaaaaa
     4801 agattgctga agaaaatttg gataggatta ttgataagat tagacaaaaa tatggattta
     4861 cagctattat acatgcttca tctatgttag aaggtgcaag aagtattacg agaagcactt
     4921 taataggagg acacgcagga ggattaggag gtttgcataa tgattgaata taacgataga
     4981 ggacgtttaa aatggtcggg attttatttg tctgatcata ctgaaagaat caatgcagac
     5041 aatgtacaac gtgctaatca taatttggca aaagaacaaa tgacgactga agagattgcg
     5101 aaaatactga gaactgctgt cataaaaaat agacccgtct gcattcaaaa agaggaacga
     5161 aacgctgaag gttactatcc agatgatgta gtaggaaaaa ttattggata tgacgagctt
     5221 ggtgtatttg tggaagaaga aaaagttcac tatgatgaaa ttaggaatgt ttctttctcg
     5281 gaattattaa aatggagtca gatatgaaca ttattaaaaa atacgaattc ggttatatta
     5341 cgtatgaaca gctaatagag gaattatggg gatatggtca acaattaatt aatgaagtgg
     5401 ggattgattg cttttgtttc tatattgaat ccggagcagg ataccataag aacagatact
     5461 atatatttcc atatccacca a
//
LOCUS       DAYYAG010000153         5058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000153 DAYYAG010000000
VERSION     DAYYAG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.156"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(303..1277)
                     /gene="sgrA"
                     /locus_tag="ACHARQ_002632"
     CDS             complement(303..1277)
                     /gene="sgrA"
                     /locus_tag="ACHARQ_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8411013.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1473..2312)
                     /locus_tag="ACHARQ_002633"
     CDS             complement(1473..2312)
                     /locus_tag="ACHARQ_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8411014.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2497..3384
                     /locus_tag="ACHARQ_002634"
     CDS             2497..3384
                     /locus_tag="ACHARQ_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8411015.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3978..4672)
                     /locus_tag="ACHARQ_002635"
                     /pseudo
     CDS             complement(3978..4672)
                     /locus_tag="ACHARQ_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(4656..5054)
                     /locus_tag="ACHARQ_002636"
     CDS             complement(4656..5054)
                     /locus_tag="ACHARQ_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8411016.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1608 a         1024 c          748 g         1678 t
ORIGIN      
        1 aacaaagtac atactccttt ttatctatta tttagactta ttccgacagc tacatgaata
       61 acgtacaaat agattcgtta ctgatttttc agagatacta ccatgcgcag tgtgcattgt
      121 caattattct tgctcttatt ttccatttgt gttcctgtta tttcctgttt ctcaaatatg
      181 ttagtaaaca aaaatagatc tggcaaagct acaacttcgc caggcaatac agcactctcg
      241 aatgaaatat tcactcaaaa atctatcgtt ttattcaatt ttagatctgt tgcttttatt
      301 ctttatttca ctcgatggaa gagagaacga aaatagcttg ctcctacaat caaaatgagt
      361 aacacaccca tccatgtaat tagcttgttc aaggttctac taccagtttc tggtaaatcg
      421 ctttttctat tatctgtttc tttcgctttt tcttgatgat caattccacg agaaacaaaa
      481 tcaggctgtt ttagcacact ttttaggttg ctccctgcat ttctcttttg ttccttagtt
      541 ggtatgatag gtaccaaatc aagttcatta tttacttttc ctgtgctgct ttcgctgctc
      601 gtgcttcctg tgctactctc actactcatg cttcctgtgc tactttcact actcgtactc
      661 tccgtactgc tttcgctgct cgtgcttcct gtgctacttt cactactcgt actctccgta
      721 ctgctttcgc tgctcgtgtt ttctgtgcta atttcactgc tcgtactctc tgtactgctt
      781 tcaccgctcg tgttttctgt actgctttca ctacttgtgc tatccgtact gcttggcggt
      841 ccaacaactg tccaactgct tggtttcaaa ttctccgtga tcgtcatctg tccatcataa
      901 aaaacaatca aatcaacttt atctttactc ttgatctcat caatcgatag ttctgcagaa
      961 atcattattg tatactcaat accagcggtc cattgtacgt cttttaaata aacttcccca
     1021 ataacttttt ctgaacttcc attaccatcc caaccatatg ttagaacagc atgtacatca
     1081 ctagcattca cgcttttgtt tggtttgact ggaatagtaa tgacttgttg tgtcatgtca
     1141 taaacaatcc gatcagtttt cacagtaaat gaaagattct catttgcccg ttcatttgca
     1201 gaagtactga ttccaatcat tgctgcaatt atactcagcc caagaataac aaataatacg
     1261 gtcgctgttt tcttcatttc tatcactctc catataatta ccttctattt gataaaggaa
     1321 ttatataaaa tgaaaatgat gagaacacga cagtcagttg tgaaaatcta acaaaagatg
     1381 tcgtgtattc gcttgaattt gtttactttt tcgtttaatc aatgacagac ttaaaaaagc
     1441 aagaccgata tcagccttgc tttcttcatc ttttattgat aaacagcagt gtatttcttc
     1501 atccgctttg ttttatatgc atagagtaac aaaccaacaa tcaagactaa caagaataat
     1561 gtagttgata tagatgcttc agccccaaag tttcccccag taattattgt attttgcgat
     1621 tggatcagag tggagaaaaa accttgttct tgcgttccgc tgactggtat acccaaaata
     1681 gctccttgtg tccaattcca agtaatatga gcaccaatag ccgtaaataa acttcctgta
     1741 ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac tgccagaaat
     1801 gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc aatcattgct
     1861 attaacggac caaatctaag catctgatgc tgaaaaaagc cacggaataa aagctcttct
     1921 gatgccccca ctgcaataaa taaaataaaa ctcgttatca gcggcacaat gtttgcgttt
     1981 cctgaatcta accgatacgc ccccgtcaaa tataatggac tagtaataag cagaaacact
     2041 ccaattccaa caacgagacc tcccgctaaa tacccccagt cttttcctcg aacagaaaca
     2101 ccataatctt ctattctttt tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa
     2161 acagctaaat gtccgaccgt accggcaatt gctccaatcc attcactatt tccaataaac
     2221 atatccgcaa gtgcattatt gacaagaatt cctaaaacaa aaccaacaaa gactattaaa
     2281 acagatccaa tcaaccgaat aatcctgatc atctttcatc cctcctttat attaagttta
     2341 acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa
     2401 tagattttta cgaactaaga atatctacag caaccttacg aaaatgaaag actgtctccc
     2461 taattcttct gttagactgt ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa
     2521 ctactaatta tgtgcctttc agcatgtgca attttactat tctcaggttg tagcaagtcc
     2581 aatgaaacga ttgcaacgtt taaaggtgga acacttacag ttcaggattt ttatgataac
     2641 ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt
     2701 gatgatgtat acggaaaaga gatttcttca aaggaagtaa caaaagaata taatgaacaa
     2761 atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag cagttggcca aacagaagag
     2821 acatataaga aactctttaa acagatgctg gctttccaat acggtttgaa agccaatgta
     2881 aaattaacag ataaagatct agacactgct tggaaagaat tctatccaga agttagtaca
     2941 cagatcattc tattttcgac taaagaagaa gcagataaag caaaaaaaga agcaaatgaa
     3001 ggagaaaatt tctccaagct tgttcaagct tatggaaaaa ataaaacatt aaaggaaact
     3061 gatggaaaaa tgaattttga ttcaaccaat ccagagattc ctactgaagt aaaaaaagca
     3121 gcatttaaac taaaaaacgg ggaagtttct gatatcattc ctgtaacaga tccaacgact
     3181 tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa
     3241 gacaaataca aatctgagct agaaaaaatt gctaccgaag caaaaactct tgatacggaa
     3301 ttcatggata aaacaatccg taaagtgatg aaaaaagaca atgtaactat taaagatcct
     3361 tatgtaaaaa atatattcaa ataaacaaat ttgacccaat tggggatgtg gcaaaatttg
     3421 agaagtgatt tttggaaatt ccttcttatt tttgaggcaa accacttttg ccacaacttc
     3481 atcggggcct ttttttattt gtaccaaaac aaatcactgg atttgttaca atcatgagga
     3541 tataatttaa aaaagttttt tatttctatc acatgaatca atgcgccatt aagtagattc
     3601 tttcatgctt ccctattttt tcacttgccg tatcgcttca aaaacaacta tcataaactc
     3661 aaactcgact gaaaagttat tttaaaaaag actaaaaata tcccttctgg acttgttctt
     3721 cttattctgt tatactagag aaacacgaaa gatattttta tgcgacactt ttcatttgtt
     3781 tttatgaaat ttattcaaaa ctggcgtaac ttcagagtta caaggatgta ttagaggtta
     3841 gggaatacat cgtttaaatg agaaataagt aatagttagt aatagtcacc caaactttaa
     3901 attaatgaat attgaatcgt cagatagcct cctcgagttt tcgagaaggc tatcgtttat
     3961 tttatggaga aattgattca gaagtaatca gcgacctttt ttagttcacc aatcgtttct
     4021 gcttggaaag tttgcaccca atcttcttgg aagcccctca ctgctttatc aatcgagggc
     4081 atctctaata cttgatctag tagctcttta cctaacgtta tcgctgagct ccagatcgaa
     4141 tggctgtagt gacttgttca caatttttga aactagctgc taatattttt gttggtcttt
     4201 gcgtacgttc gatctcagtc cgcaacgaat caatcacttt catagcatca atgccttgat
     4261 tttgcatcct gttataataa ggtgcaatat aatctgctcc attattaata gctaaatacc
     4321 cttgtaattc tgtgtaaata gctgtaccgg taatcaaata accttctttt ttcaactttt
     4381 tgatcgcttg aaggcctgct tgattcaccg gcaccttgat aaacgtatct tttcccagtt
     4441 cttctatgat aactcgagca tcctctatca tttcttcttc cgttcttcct attgtctgaa
     4501 tatggatagg aatctgtccg attatttttt tgatttcttt catatgcgag aagaagtttg
     4561 acgctccttc ttttttcaga attgtaggat ttgttgttac gcctgttaaa ttcaaaaact
     4621 gttgacaaga ataaatactt tttgtatttg ctgtatcaag catgatttcc atatagcttt
     4681 cccccattct gatcctcttg atcgatttta atgattgtcc cctttcttaa atgcggcatt
     4741 tccttatctt ctacatacat tgttcctgga agttccggct catgagatcc atcaaatttt
     4801 atcgtgatat gtcctaattc attcaaatta gtttggacaa catctcctac tgctgtgatt
     4861 acatacattt gtttatcaaa acagagcttc atatcacaag ttatttcttt tcttacctga
     4921 ttcaattcaa tcttataaca ataatctgtt aatgtttccg gtgcttcatc tccaaataaa
     4981 atcattattt tttctttcct gaatacttct gcttctatac caacatctgt tacaaccgtt
     5041 tcaaagatcg tcatctct
//
LOCUS       DAYYAG010000154         5005 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000154 DAYYAG010000000
VERSION     DAYYAG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.157"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHARQ_002637"
     CDS             complement(<1..182)
                     /locus_tag="ACHARQ_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8411017.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHARQ_002638"
     CDS             517..951
                     /locus_tag="ACHARQ_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8411018.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHARQ_002639"
     CDS             974..1294
                     /locus_tag="ACHARQ_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411019.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHARQ_002640"
     CDS             1435..1626
                     /locus_tag="ACHARQ_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411020.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHARQ_002641"
     CDS             complement(1697..1954)
                     /locus_tag="ACHARQ_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8411021.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHARQ_002642"
     CDS             2112..2375
                     /locus_tag="ACHARQ_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411022.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHARQ_002643"
     CDS             2399..2587
                     /locus_tag="ACHARQ_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411023.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHARQ_002644"
     CDS             complement(2670..2855)
                     /locus_tag="ACHARQ_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411024.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHARQ_002645"
     CDS             3070..3441
                     /locus_tag="ACHARQ_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8411025.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHARQ_002646"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHARQ_002646"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHARQ_002647"
     CDS             complement(3709..4137)
                     /locus_tag="ACHARQ_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411026.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHARQ_002648"
     CDS             complement(4197..4955)
                     /locus_tag="ACHARQ_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8411027.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1743 a          784 c          978 g         1500 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttt
//
LOCUS       DAYYAG010000155         4996 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000155 DAYYAG010000000
VERSION     DAYYAG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.158"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1880
                     /locus_tag="ACHARQ_002649"
     CDS             165..1880
                     /locus_tag="ACHARQ_002649"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8411028.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1883..3646
                     /locus_tag="ACHARQ_002650"
     CDS             1883..3646
                     /locus_tag="ACHARQ_002650"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8411029.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3686..4222)
                     /locus_tag="ACHARQ_002651"
     CDS             complement(3686..4222)
                     /locus_tag="ACHARQ_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8411030.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     regulatory      complement(4260..4358)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(4545..4967)
                     /gene="mgsA"
                     /locus_tag="ACHARQ_002652"
     CDS             complement(4545..4967)
                     /gene="mgsA"
                     /locus_tag="ACHARQ_002652"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8411031.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1493 a         1009 c          970 g         1524 t
ORIGIN      
        1 tctttttttt aaaaaaaata gtctattcaa tttctaaagc ttcttcataa taaaactttc
       61 atttatgaaa aggcttgttc tcttttcatt ctaaatttcc taagctacaa tgtggtagta
      121 cttgtttttt caattgtgga caaataaata ataggaggaa tcatatgatt cagttattac
      181 gttatgcaaa agactatcgt aagcaaatca ttttgggccc gttcttcaaa tttcttgaag
      241 ctgtcttcga acttgtcctg cctttaatga tggcttcctt gatcgataat ggactaaaaa
      301 tgaatgaccg aggaaaaatc attgaaatgg gcttatggat ggtcgctatg tctgtgattg
      361 gtctgatttg tgctatcatt tgccaatact atgcttcagt agcttcacaa ggatttggta
      421 cagagttacg taatcagcta atcaaaaaaa tcaatacttt ctctcacaac gagttgaatc
      481 attttggaac agataccttg atcacccgaa tgacaaacga catcaatcag cttcagctag
      541 ctttagcaat ggtcattcgt ttattgatca gagcaccgtt tttaagtatc gggtcagtgg
      601 tcatggcttt tgtgatcgac tgggaagtag gtctgttttt cttagcatta ttgcctattt
      661 tctcaatcat tttatttttt atcatcaaaa aaaccgttcc tctctaccaa aaagtgcaag
      721 aaaaactaga tcagctcaat gaacacgtta gtcagaatct tagcggtgtt cgtgtcatcc
      781 gcgcatttgc gcagacaaaa aaagaaacag agaagttcga taaaagcaca aatgatctag
      841 cgaacaatta tttacgtgtt tccaatatct ctgccctatt atcacctgct acaaccttga
      901 tcatgaatgt cgggatcatc tgtttgctga cagttggcgg gatcaaagta aatattggta
      961 gcttgcaaca aggacaagtt cttgctttga tcaattatat gaatcaaatg cttttggcac
     1021 tgatcgttgt ttcaaacctc gtcgtgatct tcacacgtgc cgaagcttca ggaaatcggg
     1081 tgaaagaggt gctagacaca gaaaattcga ttcttgaagc tgaggtcagt actactcccg
     1141 atttttcttc tgaagcaatt atcgcttttg accatgttga cttccgttat actcctgatt
     1201 ctggtctttc tctccaaaat attacatttc agctgaagcg aaatacagtc cttggaatca
     1261 ttggtccaac cggaagcggg aaaacgactt tgacacaatt gattccgcgt ttctatgatg
     1321 tcagttccgg tacagttata atggacggaa tggatgttcg ttcttggcag ctggatcatc
     1381 tccgtcagca aattgctctt gttccccaaa cttctgtact atttaccggg actattcgtg
     1441 aaaaccttca atggggtaag aaagacgcca cagaaaaaga atgttgggaa gctctgaaaa
     1501 tcgctcaggc agaagaattt gtccgtcagc ttccaaatgg tttagataca aaagtcatgg
     1561 aaaatgggaa aaatttctct ggcggacaaa aacagcgact gacgattgca agagctttga
     1621 ttgccaagcc agcattactg attttagatg actctttaag tgcgttggat tatcaaacag
     1681 acttggattt gcgtaaggcg ctacagactt atcttggcag tacagtgatt attatctccc
     1741 aacgtgtccg ctctattcaa gaagcacatc atatcttagt gatggatcaa ggtaagattg
     1801 ctgctcaggg tacccatgaa gaattacttg ctcaatcagc agaatatcag gaaatcgtcg
     1861 catcacagga ggaagaatag ttatgaaagt aaaaaaatta tccttgcaga cgctccgccg
     1921 attcattcct taccttactg cctatgcaaa agacatttgg ctagctgtga tactaggaat
     1981 catcaatggg attgcaacag ttgtgatgac gttacaaatt ggtcaaagta tcgatcaaat
     2041 gattggagaa ggacaggtcc gcttcaatcg actgttccat ttacttattc tattcggtgg
     2101 tatcgtttta atcaacgtag tcagtcaatg gctcatccaa gtgttaagca atcggttagc
     2161 ttacttatcc gttgcaaaat taagaaaaga tgcatttgct catctgaatc gattgccact
     2221 tcgttattat gatcaaaact cccacggaaa tatcgtcagt cgatttacaa atgatatgga
     2281 caatatttcc attgcaattt ctgcggcctt caatcaatta ttttctggag tggcaatcat
     2341 cattctttcg tttattttta tgatgcgtct aagtccactt ctgacattgg tcgttctttg
     2401 ttctacacca attattttcc ttgtgagttg gatcgttgct cgtgcttcac aaacaaattt
     2461 cgatagacag caaaggatta ttggggatat ctcgggcttt gtgaccgaac gaatcggtaa
     2521 ccagaaaatc gtgaaagctt ttcagcaaga agaagctagt caacagcaat ttgaactgct
     2581 aaatgctgat ctatatgaaa aaggacaacg ggcacaattt tcttcttcct tgacaaaccc
     2641 gtcttctcgt tttgtagatc accttgctta tttatcgatt ggtttagttg gcggcctatt
     2701 ggcattgaag agtggttcgt caatcactgt tggtgtgatt tccagcttta cgatctactc
     2761 aagtcagttt tctaaaccat ttatcgaact ttctggaatc acgacacaac tgcaaaccgc
     2821 tttttctggt ctagaacgag catttgattt gatcgatcag ccagaagaaa agcctgattt
     2881 gccagatgcc tatgccttgg aacccaaaaa tatcaaagga aaagtctctt ttgatcatgt
     2941 ttctttttct tatgacccca agcagcgttt gatcgaagat ttcaatttta ctgcttatcc
     3001 aggtcaaacc attgctatag taggaaaaac aggggccgga aaatccacac ttgttaattt
     3061 gctgatgcgt ttttatgaca caacaaaagg aacaattaca gtcgatgacc atgacatccg
     3121 ctctatccaa cgtgatacat tacgtaaaag ttttggaatg gtgttacaag atacttggct
     3181 atttgacagt acattgcgag aaaatcttcg ttttgggaat ccggaagcaa gtgacgcaga
     3241 aatcaatgat gccctaaaag cctcttatat gtatgaattt gtagaacgtt tgccggaaaa
     3301 attagatact aagattggcg gtcaagggtt aaagatatca gatggacaac gccaactgtt
     3361 gacgattgct cgaacgatga tcagtaatcc ccctatgttg atcttagatg aagctaccag
     3421 ctcagtcgat acattgacag agaaaaaaat ccaacaagcc ttcttagcaa tgatggaagg
     3481 aaaaaccagt tttgtgattg cccatcgcct gtccacaatc aaaaatgcgg accagatatt
     3541 agtgatggat cagggagcta ttgtcgaaat gggcacccat gatgagctgc ttcaaaaaga
     3601 tggttattac cgacggctct atcaagcaca attcacagca caataaaaac aagcagtctt
     3661 ctgggatggg aagactgctt gttttttatt tagcgtggat cgctaaactt cttaaaattt
     3721 ttttgtaagg aatactacgt cctacgtaat aaatggcaat tgattgaatc ggtaaccata
     3781 ttactgtttt taacaggcgg ggcgcccaaa cgacccagct gaacaatggc acatggtaca
     3841 tcaacgcaag ccataatggt gtcaaaaata agttgatgat cagcgtgaca ctaagcgtag
     3901 ccagaatggc atttttccaa gtgatctctt ttcgatagaa aaagaatccg tagattgctc
     3961 cttcaataaa tgcattcaat gtaaatccaa taaagaatgc agatttcgag aagagggcca
     4021 ttcctactaa atcagctaaa actgcgccta tccctgacca aaaaggacca aaaagaatgc
     4081 ccatgatcac ttgtggaata aatgtgacac tgattttaat aaatggggtc tcgattgcta
     4141 ccagtcggga caaagtgacc atcaatgcaa tcaataagcc cataatagca atcatacgtg
     4201 cgtctaaccg gtgtttcttc atttttagca tctcctttaa gagctgctac atcgtatcat
     4261 gcagcgaatg cgaaaataaa accgcgaaca ttacgtcctt cgtccaagag gcaacatccc
     4321 atcctcttag cacttaacgc tttattttct actctgtatt atttttggga tcttttccca
     4381 cagaaactat agcatatttt cacatttact gcaaatacga aaaactaata ttattgttta
     4441 tattatccaa tacgttcgta ttatatcgca tattatcagt ttgtttagct tttatagcta
     4501 catataggcg aacattaaac gttatttttc catacgctta tccatcaaaa tgaaagtgga
     4561 tgcttgtcta tttcatggac gattcttcga aaatctagga agccggcttc cagtcctctt
     4621 agtagcacct cagctgttcc gatattcgta gctaacggaa tctcatagac atcagataaa
     4681 cggataagtg ctgttacatc tggttcatgg ggctgtgcag ccagcgggtc tcgaagaaaa
     4741 atcaccaaat ctaactgatc ctccgaaatc atggctccaa tttgctgatc cccgcctaac
     4801 ggtccggatt taaaccgatg gatcggcaag ccggttgctt ccgtgatcct ctgcccagtc
     4861 gtcccggtag caaaaagttc atgtttttcc aatattgttt tatacgctgt tgctaatttc
     4921 accatcatag gtttttttcg gtcatgagcg atcaaagcga ttttcataag agacactcct
     4981 tttgatattg ttaaca
//
LOCUS       DAYYAG010000156         4991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000156 DAYYAG010000000
VERSION     DAYYAG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.159"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACHARQ_002653"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACHARQ_002653"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8411032.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACHARQ_002654"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACHARQ_002654"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8411033.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACHARQ_002655"
     CDS             2594..3379
                     /locus_tag="ACHARQ_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8411034.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACHARQ_002656"
     CDS             3392..4243
                     /locus_tag="ACHARQ_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8411035.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACHARQ_002657"
     CDS             complement(4367..4819)
                     /locus_tag="ACHARQ_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411036.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1689 a          815 c         1039 g         1448 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat a
//
LOCUS       DAYYAG010000157         4920 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000157 DAYYAG010000000
VERSION     DAYYAG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.160"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHARQ_002658"
     CDS             <1..107
                     /locus_tag="ACHARQ_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8411037.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHARQ_002659"
     CDS             complement(176..904)
                     /locus_tag="ACHARQ_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8411038.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHARQ_002660"
     CDS             complement(917..2197)
                     /locus_tag="ACHARQ_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8411039.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHARQ_002661"
     CDS             complement(2338..2940)
                     /locus_tag="ACHARQ_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8411040.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHARQ_002662"
     CDS             3185..3391
                     /locus_tag="ACHARQ_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411041.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHARQ_002663"
     CDS             3703..4422
                     /locus_tag="ACHARQ_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8411042.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..>4920)
                     /locus_tag="ACHARQ_002664"
     CDS             complement(4503..>4920)
                     /locus_tag="ACHARQ_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HFM8411043.1"
                     /translation="LFVLYYEAVIFFFVSLTPVHEHPALVTILIAFTASIQWVAFDKI
                     NGRAYTNLFTTGNLKGVATNLYDYCISKEKADFDRFFHYLSVVLAFIAGAIVLVFCHH
                     LFGAHSILIVSILFLIMALTQTFHLWQFYHTNRFTI"
BASE COUNT         1707 a          828 c          919 g         1466 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
//
LOCUS       DAYYAG010000158         4898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000158 DAYYAG010000000
VERSION     DAYYAG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.161"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..314
                     /locus_tag="ACHARQ_002665"
     CDS             15..314
                     /locus_tag="ACHARQ_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411044.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            579..1454
                     /locus_tag="ACHARQ_002666"
     CDS             579..1454
                     /locus_tag="ACHARQ_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411045.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1451..1669
                     /locus_tag="ACHARQ_002667"
     CDS             1451..1669
                     /locus_tag="ACHARQ_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411046.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1656..4283
                     /locus_tag="ACHARQ_002668"
     CDS             1656..4283
                     /locus_tag="ACHARQ_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8411047.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4298..4516
                     /locus_tag="ACHARQ_002669"
     CDS             4298..4516
                     /locus_tag="ACHARQ_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411048.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1914 a          612 c          981 g         1391 t
ORIGIN      
        1 aaggagaggt ttttgtggaa gcaataaaat taatgatttc agaagaacag gaggaaatgt
       61 ttctaaagca gttttatcaa attgctacaa aagctattga aaaggctcaa gaaaatgcgg
      121 ggatgacaag ggaatatctc aatcaaaaag aaatggcgga atacttaaat gtgagtgaaa
      181 actttttaaa aaatgctgtg agaaatgagg gattgcccgt agttgaatta ggggcaagaa
      241 aattttattc gaaaaaggca gttaatgagt ttatgtttgc tcgaatgaaa agaggggaaa
      301 atattggaag atgatgaagt agaaattata aaaaaatgct ttaaaaaaag aaaaaaagtg
      361 ttcccaaaag tttgcagact gggggaacac tcaataataa agtgctgaga aaactttatt
      421 tatagctatt atatcaaaaa agaaggtgaa aaggtgtttt cttctggaaa tgttgtagtt
      481 gacaaactta gagaaataaa ttttgacgga aatgtaatcc acacacatgg tacgatcaat
      541 taagaaaaaa aacgaaaaaa ggtgttgaga agccctattt gaatgcaata gttatcttag
      601 cagaaattac ctattggtat agacctaaaa aaatttttaa cgatgatggt cagctacttg
      661 gttatcaaaa aaagtttctt gaagatattt tgcaaaaatc atataagcaa ttaagcaaaa
      721 aaacaggatt atcagaacga gctataaaag atgcaattgt gtacttagag aaaaagggga
      781 ttatcaaaag ggtttttaga aatatttatt atgagaaaag agcagttaca ttatcaaatg
      841 ttatgtacat tgttttaaat gtacataaat tgatagaaat tacttttcct actttggatt
      901 gcgaaaaaaa ggagtatatg ccgaaaattc gtcagagggg tatcgaaaaa aagagatatg
      961 tctctttaaa aaaaggtaga ggtatgtaca aatttgtata tacgtataca aagactactt
     1021 cacagaacat atcaaagact actctcgtta tggaagaaga ggatattaat ataaagagga
     1081 tttacagtaa gattagtgtt ctttatgacg caaataacga agatggtcga aaattatttc
     1141 aagtgtatca ctatttgaga aacgctaatg tagatacaga agatatcaga aaaatcattg
     1201 aatatttatc tgaaaatact cagtatctta ttgctgagtg tattataaaa caacatgaag
     1261 catgcttttt tcaaagaaaa actgaagaag ggttatacga ttatgtaaca tattttttga
     1321 atggacttca gaaaagatat aaaagtcaat ttttagaaaa tgtagagcct atagatcagt
     1381 tcaaaaaagc tttcgaaatc aaagagttac ctgaaattac tatgcataat tggttgaaag
     1441 gagaagaatc atgattaaaa atcgcttaat agatcaaatg tggtgtcttg atcaaaatac
     1501 agatactctc gaattaatcg agagtatctg cttcaacaca gtagttctcg atttaagagc
     1561 tgaaaatgac aattgtgtaa ctcatatcat aattaatcaa aaaatggctc agcagctctc
     1621 tgagagccta agaaaatggg ctgaaggaga aaactatgaa agcaattaaa gaaaaaatcg
     1681 ttagtagaaa aataaactgg ttagcactca gtgtacttgt tttaatgatt agcgttgttt
     1741 ttacaagtaa tattgttgaa gctagcattt tcaattggtt tgatgacaca aaaagtcaaa
     1801 cagagttttt gaataattct ctttatagac cttacctaaa aaaatacaat ggagatttcc
     1861 ttagtcagtt cgggatttgg cttggatggg ctgtggtaaa aggcatgttt actgtgactg
     1921 attcgattca aaatatgatt ccagatgtgt tggatttgtt caatttcatt gaaagtacag
     1981 ggctaaataa tgtttatcaa tcagtcataa atacaattgt cgttggatta atgatccttt
     2041 cattgatgtt tgttggctac aagatgatta ctggaaaagg aacaatagat ttaagatctg
     2101 ttggaatgaa tatagtgatg tctgtagcgt taattctttt aatgccaaca atgatttcta
     2161 gcggtattca atttgccaaa atattttaca atgatgctac tacgattaca aattcagatg
     2221 acggagtcgc ttggtcattg ataaaacaag gagtaacaga tctggcatat attaataaaa
     2281 cagatcaata ctcaagcatt gataaaacgg aggatcgaaa taagttaaca cgaaaaaatt
     2341 ttcaacagac agatttaaca caagtgttga cggataaagt gattgataaa ttggaaaaag
     2401 aaaatccagc tgcggataat cttcgctatg aattagttga aaattctaat aatgaatttg
     2461 ttgccacaaa gttttctgat aacttcttat caacattttc tgatagcttg aaatcaggtt
     2521 attatcgata tcaagcaaat ctttggggga tttcgattgg gttgacagcg ttggcaatcg
     2581 cctatgtgtt cagcgcgttt gtgatcatta cagcaatatt agaacttgca tttaaacgtg
     2641 tgttaggtgt tcttgttttt gcgacagata ttgaaacagg acaacgttca aaagttgttt
     2701 tatccgatat tttacaatgt tacttaacag ttggatttca agggtttgga ctatctatgt
     2761 ttgctatgtt tatcaatttt ttaaatgctg gacaaggtat ctccacaaat atttttataa
     2821 aaacaattgc ttatatctgt gctgtgtttg tgctgataaa aggcagtgga acagtcatga
     2881 gatactttgg tgttgatatt ggactcaaag aaggctatgg gcaacttgct tctgcttttg
     2941 gtatgggcgc aatgctgttt agaaaaggat caactggatt taatcgtgct aaaggaagcg
     3001 gaaatggaaa tggatcagac agtggagaag gagaagatcg aaaaccagaa aagaatttcg
     3061 gcgaaactct atcaaaaaaa gctggcaaaa ctggaagagc tttgggatat gcccatgaac
     3121 gtggattatc aggtttagct tcagatggag ctacaatggc tagtgaaaga gcaataaaac
     3181 catttaagag tatgcgtgat atggctaatg ataccaagaa taagttcaaa gaaggtttag
     3241 atgatggtac agtgtccgct attaataaaa atagcaaacc tatgttagct aaaaataaag
     3301 aagatgaaac tggaaaatat gctgattcca tgccaacacg tctttcggat agaaaagatg
     3361 gtgctaaggt tgaaaatgcc gatagaatta tgtctagttc tgaacgtatg cgtgaagcaa
     3421 tgaaaaataa tgctgaatcg aataataatc ctgtaagtgc aattcagcaa aaagttcaac
     3481 aagatattga agaacgtaaa aatgctatgc acggtcaagc taagtctgct gaagaattaa
     3541 ttaatcagaa gcgacaagaa gctaaataca cgcctgaagc aatgaatcgt gaggaaatgt
     3601 taaggaaacg tgtagaaggg cgtacaggag ctaatacgga cgaaaaagaa gcgttgacca
     3661 aagaaagaat ccaagaagct aaaaactcaa ataccggatt agaaaaattg gtcaaagaaa
     3721 atctacaaaa ttctacaagt agtcaaggtg gccgatcagt agatgtccga gaaaatattc
     3781 aaggttcatt gaatggtcat gtcggacgga atgttgatgt aagagagaat ttacaaagct
     3841 cttcaaatgg tcaaggtact aagacaattg atgttcgaga aaatgtacaa aattctacaa
     3901 gtagtcaagg gtttaaatca atggatgtaa gagagaatgt acaaaattct acaagtagtc
     3961 aagggtttaa atcaatggat gtaagagaga atgtacaaag ttctacaagt agtcaaggat
     4021 tcaaaacagt agatgttcgg gagaacgttc aaaaagatag tggagaaatg aaagaaaaaa
     4081 cacaaaaaat caatatcgtt gaagacagaa agtcagcgaa aaaatttgat tcaaatcatg
     4141 aaacagtgat tattgatagt gaaatccgtg aaaatgataa gggatcaaaa ccaagacgaa
     4201 gatttaccta tgaagacaac gagttattca gagatacact taatgatcct aatccgcttt
     4261 tcgataatct attaaaaaaa taaaaggggg aattgtcatg aaattaattc taaagattag
     4321 tacgcatgat ttcttagaac ttgacgaatt atgtgaatca gtaaagaacg caacaaatga
     4381 aaaaatcacg gtcactaatt atattgaact gatgattcaa gaagaattaa agatgaaaca
     4441 tgagataaaa aacaacttga aatattgtta tctagaaaaa agtgaagaga taatccatga
     4501 attaaatggc ttttaaaaat tgtatttgac atgaatttac gacaaggaat tagtggggat
     4561 taagcgatta ttctcttcca ctttagagaa ataatgggtt aaaaagccag aatacgtaga
     4621 gcaatattga caacctataa aggagctatt gttgaaaatt gaagtaacaa aaagataaag
     4681 ggaggtttac atctatcaga ttaaaagtga tagtacaccc atgaaacctg caaaagagtt
     4741 gaacctaatg tatatcggtt taactctttt tatttgcaaa ttaaaaaaat atcaccgaag
     4801 tgacgaatga aactatgacc aaattaagca agaaaatata tcttgcaaat aaagtagtat
     4861 aaaaagatat tgataaagcc aatcaaaact ttgaattt
//
LOCUS       DAYYAG010000159         4831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000159 DAYYAG010000000
VERSION     DAYYAG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.162"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..607)
                     /locus_tag="ACHARQ_002670"
     CDS             complement(65..607)
                     /locus_tag="ACHARQ_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8411049.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            742..1131
                     /locus_tag="ACHARQ_002671"
     CDS             742..1131
                     /locus_tag="ACHARQ_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411050.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1135..1899
                     /locus_tag="ACHARQ_002672"
     CDS             1135..1899
                     /locus_tag="ACHARQ_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8411051.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2028..2975
                     /locus_tag="ACHARQ_002673"
     CDS             2028..2975
                     /locus_tag="ACHARQ_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8411052.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3105..3845
                     /locus_tag="ACHARQ_002674"
     CDS             3105..3845
                     /locus_tag="ACHARQ_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411053.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4136..4804)
                     /locus_tag="ACHARQ_002675"
     CDS             complement(4136..4804)
                     /locus_tag="ACHARQ_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8411054.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT         1647 a          820 c          945 g         1419 t
ORIGIN      
        1 taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt tttaagtttg
       61 tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct gaaatatatt
      121 ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg cgatacagtt
      181 cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct tccagcccca
      241 ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc aaacttttat
      301 cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat gccaatttgt
      361 cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag acaaataaat
      421 tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa atcgtgatct
      481 cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac atgaaacgta
      541 acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac atttttggat
      601 caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa atcgtaagtg
      661 aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa tagaattata
      721 aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc cagccacgtg
      781 ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc aattgaggta
      841 gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt ttttttcttt
      901 ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa tttttctatt
      961 tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg cttatttccc
     1021 gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca gacaactgtt
     1081 ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta acaattgaat
     1141 ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt tctgaaagaa
     1201 ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac aagtaaggag
     1261 ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat cctgccagat
     1321 gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta tacattttgg
     1381 gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt agaaaaaaga
     1441 ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat gacaacatgc
     1501 gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg ttcccctgca
     1561 cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta tgtcgtacca
     1621 aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt gcagccggaa
     1681 aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa aatcccttat
     1741 atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa agaaattgtt
     1801 tttcgttcct caaaaacaca gcgacatatg gtacaaatac aactaatgga agaagcttcc
     1861 acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa aatcactcat
     1921 gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt aggaaaattt
     1981 ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg cccactttag
     2041 cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc atcaatcatc
     2101 cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct gaactggatt
     2161 accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc atcaaattat
     2221 ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg atgggaatcg
     2281 ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat gccttcgacg
     2341 tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc gattggatcg
     2401 accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat gattatgtag
     2461 acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa agagattatc
     2521 aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca agggaaactg
     2581 gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg tttccaaatc
     2641 attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca gataaaactt
     2701 gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt ataaatgctg
     2761 gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt ttagaccaaa
     2821 tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt gaagtatgtg
     2881 gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat cgtcgttttt
     2941 ttcctgaact agttatcaga gagacaacga attaagtctt attactatat atatatttat
     3001 gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac aatgaatctg
     3061 tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa gaaatcagac
     3121 gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta accaacgaca
     3181 agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat cagtccccgt
     3241 ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc cgtcgcaagc
     3301 gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa aagcataaag
     3361 gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca ggacgaaaaa
     3421 aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca gagtccaagc
     3481 atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat tttgttccta
     3541 aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct gaggatcatt
     3601 taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca gaaccgacgc
     3661 cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga acaaaaacgt
     3721 tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt atcttagaac
     3781 gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat agctatttta
     3841 aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct gcggcgtttc
     3901 cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt gagtttaagc
     3961 gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa actattttcg
     4021 cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga ataatatcat
     4081 tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat aagaatcagt
     4141 gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt tttgcttcca
     4201 attggggcaa aacagatcca atttccagat acttcacttt attcaatgta taagatagaa
     4261 tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc aaggagacaa
     4321 ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga aaatattttt
     4381 caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga aagacgagac
     4441 cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga ctatctacag
     4501 caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca aatgttccga
     4561 cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc aataaaggta
     4621 aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc aaaagcaaag
     4681 caataccagt aaccccacca tctgccagct tagatggaat attgattcct gtcacagcta
     4741 atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa tttaattttt
     4801 tcatttctag cctcttttct tttgaaaatt t
//
LOCUS       DAYYAG010000160         4810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000160 DAYYAG010000000
VERSION     DAYYAG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.164"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACHARQ_002676"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACHARQ_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8411055.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACHARQ_002677"
     CDS             complement(2064..3062)
                     /locus_tag="ACHARQ_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8411056.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACHARQ_002678"
     CDS             complement(3288..3941)
                     /locus_tag="ACHARQ_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8411057.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACHARQ_002679"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACHARQ_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8411058.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1415 a         1007 c          746 g         1642 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt
//
LOCUS       DAYYAG010000161         4793 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000161 DAYYAG010000000
VERSION     DAYYAG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.165"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..733
                     /locus_tag="ACHARQ_002680"
     CDS             62..733
                     /locus_tag="ACHARQ_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411059.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            792..1232
                     /locus_tag="ACHARQ_002681"
     CDS             792..1232
                     /locus_tag="ACHARQ_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411060.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1244..1912
                     /locus_tag="ACHARQ_002682"
     CDS             1244..1912
                     /locus_tag="ACHARQ_002682"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8411061.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1976..2599
                     /locus_tag="ACHARQ_002683"
     CDS             1976..2599
                     /locus_tag="ACHARQ_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8411062.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2655..3041
                     /locus_tag="ACHARQ_002684"
     CDS             2655..3041
                     /locus_tag="ACHARQ_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8411063.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3254..4645
                     /locus_tag="ACHARQ_002685"
     CDS             3254..4645
                     /locus_tag="ACHARQ_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8411064.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1598 a          789 c         1017 g         1389 t
ORIGIN      
        1 agcatcagac tttcttttca aaatatgcta ttttaaaaat gatgaaaaaa gggggagaaa
       61 aatgaatttt ttagttgttt taaaagatac tctgattgag cgatttacat ttctatatgt
      121 tcaattgcag gaaaataaaa cattgctgca tttttcacca gagttccatc tagaggggct
      181 aacattagga gaaatctatc aagcagaagg gctgtcggct gttttgcact tttttaaggt
      241 agaattggaa ttaccagtaa aagattatat cgagctttct cttgaatctt gggatcgagc
      301 agtggttgaa ctgtttccag aaggtttaat gatcgataca aacgatggga agatccatct
      361 gactgcagca gaactgcaaa aacaaatgcg ttatcaaata gatgacgaaa attctttttc
      421 tatttttaga cgtcagcaaa agattctaaa aagcttttta aaagtgatca gtaaaaaacg
      481 gaatctgatc aaaacgacac agctgttgaa aaaatatcca ggtctgcttc acacgtctat
      541 tcaatttcct caattgatta cgatgatcca tcgttttata cgtatgcaac agctaccatc
      601 taagaagctg tttcttccat tgacggacac atttcgagta gtcaacagag aagataaaaa
      661 gaaagtgatt gtgattgact ttaccagaaa tcggaatgtc cttcaccaag ttgcaatgga
      721 aaaggccaat taaggctttt tctttttgga tttttttgtc ataataaata ttagatgatg
      781 gaggagataa tatgatgcaa acatggttag aaaggctaat acgatttcag cagaactatc
      841 acaaacttat gcagggaaga tatgcgaaat ttgatcagtt gaatcgcggt ctattgatcg
      901 ctgcgcttgt ctttagtttg ctaagtcgtt ggttgcccta tagaatcgga caattgttgt
      961 tcatcgtctt tttcggatgg atgatatttc gtttcctatc aaaaaaaatc taccccagac
     1021 taaatgagaa tcaacgttat ttgaaatatc ttgatagagt caaagaaaaa tatcatcaga
     1081 taaaaaacaa aaagcaagtc aaaatggatc gaaaaagtta tacttttttt gagtgcccga
     1141 attgtcatca aaagcagcgt gcacctaaag gaaaaggacg aatacgtgtg acatgcaaaa
     1201 catgtggtat aaaattcgaa acgaatgttt aagggagaaa agaatgaaat tagtattatt
     1261 acgtcatggc gaaagcgaag caaattttga aaattactgg acaggatggc tagatgtcgc
     1321 cttaacagaa aaaggaaaag aacaagcagc agttgccggt caaaaaatgc ttgaagccgg
     1381 acttcaattc gatgccgttt atacctctgt tttgaaacgt gcgattctta ccggacaagt
     1441 cgcactagaa gaaatgggtc agttatggct tcctataata aaaacatggc gtctaaatga
     1501 acggcattat ggggatcttg tgggtaaaaa caaagataag atgaaagaaa tatacggaaa
     1561 agatcaagta aaaacatggc gcagaggata tacggaagtt ccgccgttaa ccgcagaaaa
     1621 tagatttgac cgcagatatg atcacttgga tccgcatttg atcccatacg gagaaagtct
     1681 ggaaatgaca gtgaatcgga tcattccctt atggcaagat catctagcac caaaactaaa
     1741 agatcggcaa gatttactag ttgtgggtca tggtaatagc atccgagcac tgacaaaata
     1801 tttagaagat gttcctgaag atcaaatgga tacgatcgat atcccaaatg cacaaccaat
     1861 ccagtataca ttagcagatg atttgacaat tctggacaaa aaaatacttt aaagaacacc
     1921 tttatctaaa agtgagagat agaaaaaaga agcaaaggaa tggaacaaaa ggaacatgaa
     1981 aaattatata tgggactttg atggtacatt gtttgatacg tatcccgcaa tggtagatgg
     2041 tgcatggcaa gcactaaaag attttggtat cagtatggac aaaaaagaaa tctacttcaa
     2101 aatgaaaaag tattctacta gttatttgat caatgaatca aacttgaatg ccagagagtt
     2161 taatgaatta tttcaccgat atgaaaaaga aagtacggaa gtttcccgtc catttccaga
     2221 aacgaaacaa gtactggaaa tgctaaaaga caacggcggt cgtcatttca tcctgaccca
     2281 ccgtttgaca gaatcaacat ggggcctgtt aaaagaacat cgattggcac atctgattga
     2341 ggaagtagta gggatcgatc aggactttcc tcgtaaacct gatccagcat ctttaaatta
     2401 tttgatcgat acttttcatt tagaaagaac agacacgatg atgatcggcg atcgtcgttt
     2461 agatatcgaa gcagggaaaa atgcaggagt cgctacttgt ctttacgaca tcgatcattt
     2521 tctgggtgag atcccggcag attatgtggt aggaaatctg aatgaaatcc ttaccttgca
     2581 gagcatcatt aaaaagtagt atcccctttc aaaggagata tgttataatt ttcataaagg
     2641 agtgataaaa ttatatggaa atcagaagac aaaaagaagt tgtagaagca tttcattatg
     2701 atatgagatt gccgaaccaa gaagtagaaa cagatttgaa agtaggcttc tcgcctttaa
     2761 aagaaacgat cgaaaactat ccgaaagaac atagcatcgt aggagcacgc ttagagttcc
     2821 gtttagtgtt tgatacatat gttttatctg gaagtgtgag tcagatcaat catatcctca
     2881 atcggaaaat tgaaggtcaa aatgatgtca cacaagaaga agtggacgag ctagtagcac
     2941 cgttattcga tatcgtacgc cgaatgtctt atgaggtgac agagatcgcc ttagatcagc
     3001 ctggggtaaa attgaatttt caatcagaaa aacaagaata gacagtaaaa acatacctgc
     3061 gatagattaa agctatcaca ggtatgtttt tttattacta attgtaagca aaaagaatgt
     3121 catttcgata aaaatcgtct aaaatataaa agtcactgaa aaaaattttt tcaggaggat
     3181 ggttgaagaa tcattctcgg gaacctttac ttccttcgat ggacttgtca ttaataatat
     3241 gaggaggaga aaaatgacaa cactcatcac agttttagtg gttctagttt ttgcggcatt
     3301 attatatgtg ttctatcgta tgcaaaagcg ccatacaaaa ttttcaacac gcgtctttac
     3361 agcattagga gttggtatcg tctttggcgg tctgcttcaa ttgatctttg gtgtagacga
     3421 caaagtcgtt actcaatcaa tggaatggat cgggattgta ggtcaaggat atgtatcatt
     3481 cttgcaaatg ctagttatgc ctttagtgtt tatttcgatc attggtgcat tcacaaaaat
     3541 gaaagtttct gaaaagctag ggaaaattag tgcgactgta ttgacttctt tacttggaac
     3601 aacagctatt gcagcattga tcggaattgg ttcaacaatg ctgttcggac tccaaggagc
     3661 taaattcaca caaggagctg cagaaacagc acgaatctct gaactggcaa cacgtcagga
     3721 aacagttgaa aacttatcga ttccacagca gattttatcc tttatcccta aaaacgtttt
     3781 tgctgattta gcaggtagtc gtgcaacaag tacgattgga gtcgttattt tcgcggcatt
     3841 tgtcggaatc gcttatctag gcgtgaaaag aaaagcgcca aaagaagcag cattcttttc
     3901 agatttagtc gacagtctgt ataaaatcat tatgcggatc gtgacgcttg ttttgcgttt
     3961 gacaccttat ggagtatttg ctttgatgac aaacgttttg gcaacaagtg attttgcagc
     4021 tatcgtcaat ttaggtaaat ttgtcgttgc ttcttacgta gcattgcttg ctgttttgat
     4081 tgtccactca ctgattttga ttggtgcaaa agtcaatcca atcaactatt tcaaaaaagc
     4141 ctttccggta ttgagctttg cgtttacttc acgttcaagt gctggagctt tgccattgaa
     4201 tatcgagact cagacaaaag ctttaggagt agatgatgca acagctaatt tctcagcaag
     4261 ttttggtttg tctattggac aaaacggctg tgctggcgtt tatccagcta tgttggcaac
     4321 gatcgtcgca ccagcagctg gagtgaatgt ctttagtttg gaatttattt tgatgcttgt
     4381 ggcaattgta acaatcagtt cttttggtgt agctggagtc ggtgggggcg caacatttgc
     4441 ttcactgatc gttttagggg caatgaactt gccagtagca atcgttgggt tagttatctc
     4501 tgttgaacca ttaatcgata tggcacgtac agcagtaaac gtcagcggta gcatggtagc
     4561 tggtatcgtg acaagtgcac gcatcaaaga tttagatcgc gatgtcatca atgatgattc
     4621 taaagtgatc gatgtgaatg catagtcaaa aagaggttgt gatagaagtg gtcagcttta
     4681 agaaataaga agaagtttcc taaaaatagt ttctcaaatt tttgtgaaat ttcggcttat
     4741 ttccgagaag ctacttctga aacaccgtga ataagaggtt gtgaaaaagc tgc
//
LOCUS       DAYYAG010000162         4791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000162 DAYYAG010000000
VERSION     DAYYAG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.166"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHARQ_002686"
     CDS             complement(<1..430)
                     /locus_tag="ACHARQ_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8411065.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            488..1777
                     /locus_tag="ACHARQ_002687"
     CDS             488..1777
                     /locus_tag="ACHARQ_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8411066.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            1796..2203
                     /locus_tag="ACHARQ_002688"
     CDS             1796..2203
                     /locus_tag="ACHARQ_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8411067.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            2226..3095
                     /locus_tag="ACHARQ_002689"
     CDS             2226..3095
                     /locus_tag="ACHARQ_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8411068.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            3097..3717
                     /locus_tag="ACHARQ_002690"
     CDS             3097..3717
                     /locus_tag="ACHARQ_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8411069.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            4167..4772
                     /locus_tag="ACHARQ_002691"
     CDS             4167..4772
                     /locus_tag="ACHARQ_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8411070.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         1562 a          833 c         1003 g         1393 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcaaaacatg ggcgttatca attttatcat cgaaaatatt ttgactcagg catcgatcac
      541 cattgcattg attgctatgt tgggtctact actgcagaag aaatcagcag gacaagtcat
      601 ctctggcacg ttaaagacac tcctaggatt tcaagtgttg agtgccggtt cgagcatcat
      661 cgttggaagt ttaacttatt tcgggaaaat ctttactgaa ggatttcata tgcaagggat
      721 catcccttct attgaatcta tcaatggtca agcaatgaat gatttgggtt taggtagaga
      781 tattgcactg acgttcttag ccatttttgt attcaatatt attttggctc gtttcacaaa
      841 atggaagtac atttttttaa ctggtcaagc aattttatgg atggcaacga tgacgacagt
      901 ctttggctat tttgccggtc tgcgagggat cgtattgatc ttggtcggtg gttttatcgg
      961 agcttgtttc gccattgcta tgccggcagt agcgcaaccg atcattcgta aaatcactgg
     1021 atcaaatgat attgctttag gtcatttttg tacgatcggg tacttatttg aagccggtgt
     1081 tgctaagctt tttggtgaga aaggcgaaaa taaaaagtcg atcgaagata tcaagttgcc
     1141 gacacatttc gaatttctgc aagatactta tttgtctgtc atggtcgtga tggtcccgct
     1201 atatatcatc actgttttat ttgcaggcga accattcgct tctgaattat caggagatca
     1261 aaactatatc atgtttgcct ttttacaagc catccaattc gtagtgggtg tgtatgtatt
     1321 gctagctggt gtacgtctac tgttaggtga aatcgtgccg gcatttcgag ggatagctat
     1381 gaaattagtt cctgatgcga tccctgcctt agattgtcca gttttctttc cttatagccc
     1441 aaatgctgtc attttaggtt tcattacgac aacgattggt acgattattg ccatgtttac
     1501 tttgcccatg ttcggcttag cgatgatttt acctggtatg ctaaccaatt tctttgccgg
     1561 agggacagca gggatatttg gcaacgcagt tggcggacgc cgaggagcga tcattggcgg
     1621 tattgctcat ggtttcttta ttacattatt gcccgctttg ctagtaacga tctttaattc
     1681 gatgggattc aacaatgcta cagcaaccga tgtagataca gtagccgcag cactcttata
     1741 tgcctggatc ttaagcccag ttttgaaagc attttaattg aaagtaggga aaatgatgtt
     1801 tggatttttt aagaaaaaag aaaaagaacc gaaaaaagtt ctggcaaaag aagaaaaagc
     1861 cctatcgcca gaagcgaccg aaaagattca atcagagatt acacaattga aacaagagat
     1921 ttccactacc caagataagc ataaattagc gaaactttat gaacaagttg gacttaaatt
     1981 ttctgaattg tatgtgaacg atcaagccat ccaatattta gaaaagagtt tggaaaataa
     2041 acagacgatc ggagatgggt ataaaaaact gatgagcctc tataaccaga agcgtgcaga
     2101 tgctgctcgt gctggtgatg atcaagggat cgattactac atgggcaaaa tggatgagat
     2161 gcgacaaatt gcaaaacaag taacgatcaa aggaaacaaa taaaaagata aggtggcaat
     2221 aaaagatgta tacaacattg aaagaagtaa caaaaacagc agaagagttg aattttacag
     2281 ttggagcttt taatacgcat aacctagaaa tgcttcctgc tatgctaggc gcagcaaaag
     2341 aaatgggttc gccgatcatt attcaaacaa gtgtcgatac cgctaaatat atcggcatgc
     2401 atgtgttagt caatgtaatg acagctattg cagaagaaga aatggttgat gcagtcttgc
     2461 acttagacca cgcccgagac tttgatgata tcaaagaagc aatcgacagc ggatatactt
     2521 ctgtcatgta tgatgggtcc catctgcctt tcaaagaaaa tatcctgaag acaaaagcag
     2581 tagtagaata tgcccatgcg cacggtgttt ccgtagaagg agaacttgga acaatcggcg
     2641 gtacagaaga aggaatccat gtcgctgaaa atgacaaggt atatacaaga ccggaagacg
     2701 cagtgaaatt tgtagaagct acaggcgtgg atgcattagc tattgctatc ggtacgaatc
     2761 atggacaatt caaatcaaaa acagaagtaa acattccctt gctgaaagaa atcgatgcag
     2821 tagttgatgt ccccctagtt atccatggag gaacaggtgt gaaagaagaa gattatcctg
     2881 aattgatcaa taacgggatc cgcaaattca atgttggaac ggagctttta gtcaactgga
     2941 cgaaagtagc taaagaaacc tttagtgaaa cagaaatcaa caagtctctt cgccataatg
     3001 tgattccagc aaatgaagca gtaaaagaaa ttgtcaagca taagattgga ctgtttatga
     3061 acttggaaag tccaattaac ttaggaaata aatgagatga aaaagtattt cattcttctg
     3121 gctggtagtc ctggtacagg aaaaacatat cttatgaata aactacggga acgatttcca
     3181 gatatgtatg ccatcacgct cgatgaaatc aaggaatact atgcagaatc catcgggttt
     3241 aacaatttgg aagaacgggc ggaacaagaa aggacgaaag tttatccttt ctattacaaa
     3301 gcacttgatt tgtatatgga agcaggaaag aaagtgattg tgtccgagta tccttttagt
     3361 gataagcaaa aagcacgact ccaagaatta gcagatagat acggatatca ggtgatcacg
     3421 attcgcctaa cagcagactt tgaagtgtta tggcaacgtc ggtacaaacg tgatcgtgaa
     3481 tcagatcgtc atttgagcta tatcatggat cattatcact atggtgacgt attggaagat
     3541 cgaacgaaag ggaccaatca tattacgaaa gaagcattca aaaagatcat cgccgaccga
     3601 aaatatgaca aattccagtt aggaacacta tatgaatttg atgtaacaga ctatactcaa
     3661 gtggattaca ctccactatt ggataaatta gaaaaagaaa tcaatgacaa taagtaacaa
     3721 agaaatggct taatatcgaa aaattggctt ttgaacgctg tttaccgaga ggtcgtgagc
     3781 ctgtcgttca gctccgagtc acaaggagca atttcaaaaa aacagcttct catgtttgat
     3841 gaatattgtg acttgtgacc gaggagttgg taggagaaca ccgtttagtg gggaggttgt
     3901 gaaaaagctg tcctgcctca attaataaga acagccagac aagaatagct cgtcatattt
     3961 ttcgtttggt ggggcttatt accgcagatg caagcttttg aacgccattt attcacagcc
     4021 ccagagaagt cgaatgtctt ttgttctcga gttttttaac attcttttat ttattgcaga
     4081 tttcattata tttatttgcc gtaattaaac cagtcttcta tacttgatta catagtcata
     4141 catttgctaa aaaagaaggg aagagaatga acaagaagtt ggctcaatac aaaagactac
     4201 tcgcactttc tcaagctggt ctatattatg gaaaagatgt atttgataaa gagcgttttg
     4261 aggagatccg gacgatttca ttggaactga tcagtgaaat aggaaaagaa actttcgaag
     4321 aaataaaagc gctcacaacg attggagaag gacatccaac accaaaagta gatgttcgtg
     4381 cctatatcaa aaaagatggc aaggtcctac taatcgaaga taaacggacg aaagaatggt
     4441 cacttcctgg tggatttgct gaaataggat tgtctcctga agaaaatgtg cgtaaagaag
     4501 tctatgaaga aacaggatta acagtagaaa ctactcagtt gcgagcagtt tttgatacga
     4561 ataaacaaaa agatattcca cagctttttc agtattataa acttgttttt gcttgtgcga
     4621 tccacggtga agaggccaag tttattgaga ataatgaaac ttccaacatg ggatttttta
     4681 gtatagaaga gttgccaaag ctatcagaaa agcgaacaac gaagaaacag ttacttattt
     4741 tagaaaacaa aaaccagatc tattgtgatt gagctttttc gtaaaagaag a
//
LOCUS       DAYYAG010000163         4791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000163 DAYYAG010000000
VERSION     DAYYAG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.167"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHARQ_002692"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHARQ_002692"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8411071.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHARQ_002693"
     CDS             complement(1297..2133)
                     /locus_tag="ACHARQ_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411072.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHARQ_002694"
     CDS             complement(2317..3534)
                     /locus_tag="ACHARQ_002694"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8411073.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHARQ_002695"
     CDS             complement(3683..3844)
                     /locus_tag="ACHARQ_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411074.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHARQ_002696"
     tRNA            3917..4000
                     /locus_tag="ACHARQ_002696"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4791)
                     /locus_tag="ACHARQ_002697"
     CDS             complement(4240..>4791)
                     /locus_tag="ACHARQ_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411075.1"
                     /translation="FLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVN
                     GNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKT
                     SIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQK
                     NEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1451 a         1001 c          773 g         1566 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga a
//
LOCUS       DAYYAG010000164         4686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000164 DAYYAG010000000
VERSION     DAYYAG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.168"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1055)
                     /locus_tag="ACHARQ_002698"
     CDS             complement(93..1055)
                     /locus_tag="ACHARQ_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8411076.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="ACHARQ_002699"
     CDS             complement(1057..2496)
                     /locus_tag="ACHARQ_002699"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8411077.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..4627
                     /locus_tag="ACHARQ_002700"
     CDS             2681..4627
                     /locus_tag="ACHARQ_002700"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8411078.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1488 a          800 c          771 g         1627 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaggcacat ttaaaattga tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat
     4141 atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat
     4201 ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat
     4261 gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac
     4321 tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga
     4381 agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa
     4441 agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat
     4501 gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta
     4561 ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat
     4621 caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag
     4681 attaat
//
LOCUS       DAYYAG010000165         4652 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000165 DAYYAG010000000
VERSION     DAYYAG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.169"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1160
                     /gene="gap"
                     /locus_tag="ACHARQ_002701"
     CDS             150..1160
                     /gene="gap"
                     /locus_tag="ACHARQ_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8411079.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(1245..2180)
                     /locus_tag="ACHARQ_002702"
     CDS             complement(1245..2180)
                     /locus_tag="ACHARQ_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8411080.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            2366..3325
                     /locus_tag="ACHARQ_002703"
     CDS             2366..3325
                     /locus_tag="ACHARQ_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8411081.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(3411..4505)
                     /locus_tag="ACHARQ_002704"
     CDS             complement(3411..4505)
                     /locus_tag="ACHARQ_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8411082.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         1446 a          942 c          795 g         1469 t
ORIGIN      
        1 tattatttgg taaggtttcc aacctttttt ctgctaccaa gtataatttt ttgacaaaat
       61 attctttgcg tagcagaata acagtgtatt aattgtatta attgaggatg ccgtttcaca
      121 gcacccctaa taaaggagga acttttgata tgacagtaaa agtaggtatt aacggatttg
      181 ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt ctcagatgat attgaagtag
      241 tagcaatcaa cgacttaaca agtccaacta tgttagcaca attattgcaa tttgactcta
      301 ctcatggtac ttatcctggc acagttactg caacagaaga ctcaatcgta gtggatggcg
      361 aagcaactcg tgtttatgca gaacccgacg caagcaaaat cccttgggca aaagaaaacg
      421 gtgtagatat cgtactagaa tgtactggtt tttatacatc tgaagaaaaa gcaaaagcgc
      481 atttagatgc tggtgtcaaa cgagtagtta tttctgcccc agctggtgct atgaaaacca
      541 tcgtctataa cgtcaatgat gatacattag atgaaaacga caaaatcatc tcagcaggtt
      601 catgtacaac aaactgttta gcacctatgg cttatttctt aaataatgaa tttgggatcg
      661 aagttggtac aatgacaaca gttcatgcct acacttccac acaaatgctc ttagatggac
      721 ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc tgacaatact atcccacatt
      781 ctactggtgc tgcgaaagca atcggcttag ttatccctga gctacaagga aaattacaag
      841 gacacgcaca acgtgttcca gtcgttgatg gttcattaac tgaattggtt tcagtattga
      901 aaacaaaagt aactgctgat caagtaaacg aagcaatcaa aaaacacaca gttgacaatc
      961 cttcattcgg gtatgatgac cgtcaaatcg tttctagcga tgtcattgga acaactcaag
     1021 gttcgatctt tgacccaacg caaacagaag taacaactgc tggtgatttc caacttgtta
     1081 aaactgttgc atggtatgac aatgagtatg gatttacttg ccaaatgatt cgcttactag
     1141 aaaaattcgc taacttgtaa tttgatagat caatcgataa aagaagcctt tgactggatc
     1201 cagtcaaagg cttctttctt ttcatcccct acttttaaac tcgttcaatg aacatattgg
     1261 cttaaaaatt gttggactac ttgctgatat ttttcaggat ctgtcttgta tgcttccgca
     1321 tgctcggctc cttttacaac atacttttct tttggtccct tcgttgcacg atagacctta
     1381 tctaacattt caaatggtac aaatgtatct tcgtctccat gtataaagag cattgggcgc
     1441 tggtttttct tcaattgttc cactgcattt gcttcaccga aaaagtagcc agcacggact
     1501 tttgtcacta gactcgttac ttgcataagc ggaaatgctg gaagactaaa catatcgtct
     1561 agctgataag caagctcatc atgaacggaa gaataaccgc aatcttcaat gattgctttc
     1621 acattatctg gtagtttttc tccgctagtc atcataactg ttgctgctcc catgctgaca
     1681 ccgtatagaa cgatttcttg cgattttcca ttgttttcta gcactttgtt tatccactgt
     1741 acatagtctt tacgctctgg ccatccaaat ccaatgtaat ccccttcgct ttttccatgt
     1801 cctcttgcat caggaacaag gacattgtaa cccatatcat gaaacatctt tgcgtaattg
     1861 gtcatcgttt cagcgtttcc catatagcca tgagccacaa gagctgtttt ttcacttttc
     1921 gtttcagcag gaagataaat cgctgataat ttcagaccgt cttttgaagt gatggtccaa
     1981 tcgcttcggt tttcttttga actaaaccat ttatgtgcat ccgtttcttt ggatgtctgt
     2041 gttgtaccag gtgtatcttc gtctaaaaag tctttctcag aaggcacaac agcataatta
     2101 taaaaataat tccctgcaaa cactaacgca acaatcagag ctaaagcgac gcctaaaagg
     2161 attttcatcc aaagtttcat aaatcatcca tctcatttct cttttttcat ataaccttga
     2221 attagtataa tcaaagaatc tctactatgc aatgttcatt tttataaccc gcgaattgta
     2281 aaattaagtt agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa
     2341 agaaaacaaa ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac
     2401 aatgatagaa tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg
     2461 acgtgctata caaactattt ataatgtagt caataagttc aagcaaggca agactgctct
     2521 tgattattgg caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt
     2581 acctgctcat gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt
     2641 cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg agaacacttt atcgtttatt
     2701 ttctaaagga atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg
     2761 ccatcaggaa aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa
     2821 ttatcctgat ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca
     2881 tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa
     2941 acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt
     3001 ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa
     3061 agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca
     3121 acgcccatta aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat
     3181 ggattttaga aaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat
     3241 tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga
     3301 agcattttat tctagcttaa tttgacaaat cataacctat aaaaaaacaa aataaaaaag
     3361 cagaaataac tggctatttc tagcggatta tttctgcaat aactaattat ctatttttta
     3421 ttttcaatta atttaaagct tgaccatggt ttcaaaaagt caaggaggaa taattcgtca
     3481 tcggagattc ttcctacaac attgacacgt ccgtcgtttt tcatatcctt caaggcgatt
     3541 tgtgtttctc ctttgtattg accgtaccct tcattgtcaa tcagtacatc cccatgatgc
     3601 atatctcgcg tattatgagg cgggaaatct ttatctttgt agtatacacg agtcatcgtt
     3661 gaacgaagga tatattctga gcgatcccct cgataactat gaaggttatc aaacaagcag
     3721 attcgttcgt tttctgtaat accttcttcc gtatctactt tcaatgttgg ataatccgcg
     3781 ttaaatgctt ctgccatttc tttcaattct tcttcacttg cgtacgcatt tccaatagaa
     3841 atatcatcaa tcaatcctgt aaggattaaa tgcttcactt gtgtcgcaat ttcaagatct
     3901 cggtggtctt ccagcgtgca caagccatct tgcgtaggcc aagggccaaa ctcagcagat
     3961 tgcgaattga cgaatgccat cgtattcaaa ttatattttc tgaatttttc tgaacaaaaa
     4021 acaaagtgtt cgtatcctaa accagagtat cgatgaggat aaaaattatg agaacctaat
     4081 aaattatccg tgtttggcga ataactcatg atgttgtcaa cgtaattcgt gcccgaactc
     4141 atattgatct cgattttgat accaaaagga ttgcgcgtca tttttgcttc ttctgctcct
     4201 gtgaatccga tatctaagcg gaccccgtag gcacccatct tatggaaaaa agataagtca
     4261 tcgtaagaaa tctccaactg ttcaaataac gctggattga tatcaaccat tacttccatg
     4321 cctaggctat tcgcgtaatc aactacttct ttaaattctg aaagaacttt ttctttatca
     4381 tcattgatct gcagcaaact ggtaaaaacc cgtttatacc catacttatg agctaaatcc
     4441 aagtacgctt tgtccttttc aaatgttgac cgttctggat aaatcgagat tcctaatttt
     4501 cccatattac catttcctcc tgcatctttt cgtttttgta tccacttaca ttatagcaaa
     4561 cacaaagtaa atggtctaca ccttttcttt ttcttttaaa ataaatgata aattccaata
     4621 atttaagtag gtattttccc tctattttgt ta
//
LOCUS       DAYYAG010000166         4644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000166 DAYYAG010000000
VERSION     DAYYAG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.170"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACHARQ_002705"
     CDS             complement(12..482)
                     /locus_tag="ACHARQ_002705"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8411083.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACHARQ_002706"
     CDS             complement(641..1099)
                     /locus_tag="ACHARQ_002706"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8411084.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACHARQ_002707"
     CDS             complement(1270..2415)
                     /locus_tag="ACHARQ_002707"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8411085.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACHARQ_002708"
     CDS             complement(2428..3345)
                     /locus_tag="ACHARQ_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8411086.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACHARQ_002709"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACHARQ_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8411087.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="ACHARQ_002710"
     CDS             4437..4607
                     /locus_tag="ACHARQ_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411088.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1451 a          845 c          876 g         1472 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gctt
//
LOCUS       DAYYAG010000167         4591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000167 DAYYAG010000000
VERSION     DAYYAG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.171"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHARQ_002711"
     CDS             186..2855
                     /locus_tag="ACHARQ_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8411089.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHARQ_002712"
     CDS             complement(2946..3560)
                     /locus_tag="ACHARQ_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8411090.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHARQ_002713"
     CDS             3680..4219
                     /locus_tag="ACHARQ_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411091.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHARQ_002714"
     CDS             4216..4554
                     /locus_tag="ACHARQ_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8411092.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1521 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt t
//
LOCUS       DAYYAG010000168         4563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000168 DAYYAG010000000
VERSION     DAYYAG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.172"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            297..527
                     /locus_tag="ACHARQ_002715"
     CDS             297..527
                     /locus_tag="ACHARQ_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411093.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            686..1519
                     /gene="terS"
                     /locus_tag="ACHARQ_002716"
     CDS             686..1519
                     /gene="terS"
                     /locus_tag="ACHARQ_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8411094.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1512..2927
                     /locus_tag="ACHARQ_002717"
     CDS             1512..2927
                     /locus_tag="ACHARQ_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM8411095.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            2939..4468
                     /locus_tag="ACHARQ_002718"
     CDS             2939..4468
                     /locus_tag="ACHARQ_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8411096.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4386..>4563
                     /locus_tag="ACHARQ_002719"
     CDS             4386..>4563
                     /locus_tag="ACHARQ_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFM8411097.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEM"
BASE COUNT         1678 a          677 c          942 g         1266 t
ORIGIN      
        1 caatttttgc gctctgtctt aatgtctata tgattggatt taaaaatgga caaaataaaa
       61 aatagtagca gccgagaata attttatagt gtcactgtgg cggaaataga agacgcaaag
      121 gtggaggtag gaagcgtatg gcgcactatc gggaccttgt acaaaactag aaaggactag
      181 tacgtgtgtg gtgcgagccc tagggaggaa caggataacc gcctgtgtgt aggttgctat
      241 tacatattag atcactcttt gagtggtctt tttatttaca taaaggaggc tacataatga
      301 gaaactactg gtatatatcg ctaactaatg aatatcctcg aaccattgat gattgttcag
      361 tgcgtgttgt gcgttctgtc caaatcaaaa agaagtactc cattgttgaa atgaccagag
      421 aagctacgcc aaaagagatt gataaatgca aacttcttta ttgcggtcat ggtttctatg
      481 atgaaccaaa cattcaaaat aatattaaca agaatttgag ggattagaat gcaagaaatg
      541 gcttaaaaat ctccaatcag gacaaatcaa gttagacaaa gtttccgatt taaagatatt
      601 gattgaagct gatttaatgc taaaagatat tgaaaattag aaaacaaaac tcaacctaag
      661 aagattgcga ggtggtgtgt attgaatggc aagacaacgt gatccaagac gtgatgaagc
      721 caaaagaatt tggttagaat ccaacggaga aaagcagtta aaggaaattg catctgaatt
      781 aaatgtttca gattctcagg ttagaaaatg gaaatcgcaa gacaaatgga gcgctgaatt
      841 gaaaagtaac gttaccaatg gcaaaagtaa cgttactaat caaggtggcg ctcctattgg
      901 taatcaaaat gctaaaggta acaaaggaaa cagcagagca tcaccgccaa agagaaataa
      961 gaatgctctt aaaactggtg agtatgaaac aatatttttt gacacgttaa ccgataatga
     1021 gaaggacatc tattctagtt tgaatgatga tccttctttt gctttgtccg aagaaatacg
     1081 acttcttaaa ataagacaac ttcgaatgat gaaaagaatc caacaagctg aagctggact
     1141 aaatgatgaa gaagtcgaac gattgcagca gctaagaaag attaaaactc cgattgaaaa
     1201 aaatggtaaa aagctagaaa tcaagcgtga ggttatgcaa gatgtgcaga ttagcagaaa
     1261 aaaacatcgc aaaattgatg atattctttc aattgaagat tcattgactc ggattagcaa
     1321 ccagttagct aaagccatca agcaaatgaa tgaactttat atgaatgaat acagaactga
     1381 tttaattaaa gctcagactg ataagatcca agctgagaca aatgaaattg gcggaaataa
     1441 ttcaggtgaa gaaatagaag aatggaaaca ggcagtttta aatgccgcaa acaaacgggc
     1501 ggtgaaagaa aatgaataat gaatttattc cttttgctga tattggttct gccattgatt
     1561 attactatga taaaccagta gctttttgcc aagatatttt gcatttgaat cctgatgaat
     1621 ggcaagaaaa tgttttaaat gatttagctg aattttcaaa ggtttctgtt cgttctggtc
     1681 aaggagttgg aaaaacagca ttagaagcag gagcaatact ttggttctta acgtgtcgac
     1741 cctacgctaa agtaatagca acagctccga caatgaagca actttacgat gtactttggg
     1801 cagaggtagc taaatggtta aatgatagct tgatcaaaaa cttactgaag tggacaaaga
     1861 ccaaaattta tatggttggt gattcagagc gttggtttgc tacggctaga acagcgacta
     1921 aaccagaaaa tatgcaaggt tttcacgagg accatatgtt gattgtggta gatgaagctt
     1981 ctggtgtgtc tgatccaatt atggaagcta ttcttggtac gctatcaggt tttgataata
     2041 agctgttgat gtgtggaaac cctaataata ttgaaggtgt cttttacgat tcccacaatt
     2101 cagaccgtga taaatacaga gttcataaag tatcaagcta tgatagtaaa cgtacaaaca
     2161 aagacaatat agaaatgatt cttaaaaaat atggaaaaga aagtgatgtt gctcgtgtcc
     2221 gtatttttgg agaatttccc aaaggtgcgt tggattcatt tatcagtctt gaaacggttg
     2281 aattggctac agaaaaacaa attagtgatt ctttagtcaa taaaacaacg gttgctcata
     2341 ttggtgttga cgtagctcga tatggtgatg attctacgat tctctttcct agaattgcta
     2401 ccagggcatt ggagtatgag aagtattcaa aacgtagcac tatggaaaca acaggatatg
     2461 tcatcaacat ggccaagaat ctaatgagtc aatatccgag tattgataaa gtgatgatta
     2521 aagtcgatga cactggtgtc ggaggtggtg taaccgaccg cctagaagaa cttatagaag
     2581 acaagcatta tccttttgag gtgtttggag tgaataacgg ttcaacatca gaagacgatt
     2641 tttacgataa tttaggtact caactatggg gaaacatcaa ggaaatgtta gaagaaaata
     2701 tgacagcaaa tcttaacgga gaacagcctg ttattgaatt gccttctgat agttcgttaa
     2761 tcaaagaatt aagtactcgc aaattcaaaa tgacaagtag aagtcgtata cgtttagaaa
     2821 gtaaagatga tatgaaaaag cgaaatattg gtagtcccga tattgctgac gcactggctt
     2881 tagcgtttta tgagccacca agtcactatc aatttattca attttaggag gtgagttttt
     2941 gattagtaca gtattatctt tagagagata taagaagcta cgtgttaaat atgcaacaca
     3001 aattgaagat ggaatgtttg atccgaatgg ttttatagaa tatatgaaac cattttttgc
     3061 tgatcgcgaa agaaaatatc tagcttatac tagcgaaaag aatgaaatag acaatagacc
     3121 aaagcctaac actgatattg taaaagttaa taataaactt catgctggta tgtattcaat
     3181 tgtcgtagac caagcagtca atcattttac tggtatacct atcaaatggg attacgatgt
     3241 atcggaacaa aaaagaacac tcattcaaag attaaaagat aaattcttaa aaaatgatat
     3301 agaacttcca acagttccag aagcttttaa caaattaaca agcaatcttg attccatgag
     3361 attcgcaatg cttgattctg aaactgcaac ttttcaagga gcttgtggag tagctttccg
     3421 attgttagag cctgtggaag aagatgatgg ttggaagtta agagcaagta atattgaacc
     3481 ttggagagca gaaagatatg gaaatgccgg aatctatatc aaagaaaagt atgactctta
     3541 ccagaaaaaa ttttttcaag aaatgaaagt tatgacaaga aataaaattc tgacatatgc
     3601 ttgctatggt gatttgaatt ttgttacaag cggaacgttc aaaaaaattg atgagaccga
     3661 taaccctttg gggacgatta ccttggcaga atttaaaaat aatacgaatc gttattgtga
     3721 ttttgaagta gctgaggaaa ttggagatgc aattgatcgt gctttgtctg atcaacaaaa
     3781 cgaaatcgaa caatttaagc ttgcgtacat gcttgttact ggcacaacaa tgagcaaagg
     3841 cactgctaaa gaaatgatga atcagctagg gattatcaac ttaaaagacc ctactgcaaa
     3901 agctgaatat gtcacaaaaa aattagcaaa agatttcaat gaatatcata tggatctatt
     3961 gaaaaaacag ttttacacca tttgtaaagc aatcgatttt aacgatgagg tgtttaaatc
     4021 aaacagttct ggagaagctc gcaagtggca aatcattagc ctagaagcga aaacaaatac
     4081 taaagagcaa tattttagag aaggattgaa agaatgcgca gaaacaattg ctgcttttct
     4141 taagtttcat gacaaagtag aaattgagcc tgaaaaaatt atttttactt tctctagatc
     4201 tttgccaact gatttaagtt atttagctga agctttacct aaacttgcac catacgtatc
     4261 caaacgaact attcagagcc aaattccatt tgtaacagat gttgattatg aaaatgaaat
     4321 gatggaattg gaaagtggaa gtgcttatcc agatagtgaa tacaattttg gcggaggtgg
     4381 caatagtgac gatagaaacg aagtattgga ccaaacgtcg agaactggag gatcaagcaa
     4441 ggctcaaaca agaaaatcag acacttaaaa aattaactag tgtatttcct gaagcactga
     4501 aagagataca agcaaaacta ttatcacaag ctgacttaca caatatcact tatccagaaa
     4561 tga
//
LOCUS       DAYYAG010000169         4560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000169 DAYYAG010000000
VERSION     DAYYAG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.173"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..755
                     /locus_tag="ACHARQ_002720"
     CDS             <1..755
                     /locus_tag="ACHARQ_002720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8411098.1"
                     /translation="NKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTT
                     ASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDGY
                     YNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDP
                     NSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVV
                     DAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(870..1310)
                     /gene="fabZ"
                     /locus_tag="ACHARQ_002721"
     CDS             complement(870..1310)
                     /gene="fabZ"
                     /locus_tag="ACHARQ_002721"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8411099.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            1530..2285
                     /gene="fabI"
                     /locus_tag="ACHARQ_002722"
     CDS             1530..2285
                     /gene="fabI"
                     /locus_tag="ACHARQ_002722"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8411100.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(2395..3447)
                     /locus_tag="ACHARQ_002723"
     CDS             complement(2395..3447)
                     /locus_tag="ACHARQ_002723"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8411101.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            3573..4493
                     /locus_tag="ACHARQ_002724"
     CDS             3573..4493
                     /locus_tag="ACHARQ_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8411102.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1392 a          902 c          853 g         1413 t
ORIGIN      
        1 aaaataaagt tattttaagt attagtttag cagcagcatt gctgtttgct gctggttgtt
       61 ctgcaaacaa cacgacttct tctagttcgt ctacagaaag ttcaaaaagt acatctactg
      121 cggaaagcac gactgccagt agcgtcgatt tgaacgcatt ggaattacca caattgacga
      181 aagatgtcgc cgaaaatgaa gatctggtcg aaatggtgac gaataaaggc acaatcaaaa
      241 tcaaattatt tcccgaacac gcaccaaaag cggttgaaaa ctttatgaca catgcaaaag
      301 acggttacta caacggactg actttccatc gcgtcatcaa agatttcatg atccaaggtg
      361 gcgatcctaa aggcgatggc actggagggg aaagcatttg gggagaagga tttgaaacag
      421 agatcaacaa ccacctttat aatataagag gtgcgttatc tatggctcgc tcacaagatc
      481 ctaacagtaa cggcagccag ttctttatcg ttcaaaatac agatgatatg catgatggtc
      541 ttttaaaaga cgactatcct caagcaatca tcgacgctta taaaaacggc ggttatcctt
      601 cactagatgg ccattatact gtcttcggcc aagtgattga aggaatggat gtcgtggatg
      661 ctattgcaaa tatcgaaaca gatagttcag acaaaccaaa agaagaagta aaaatcgata
      721 aaatcaatat actgcaagaa gcaaaacaaa aataaaaaaa taacgtctga atcctgcata
      781 gatgacaacg tatgctagga ttcaggcgtc ttctttttac ataaaaagca agtaagcttc
      841 cttttgtttt gctatatcag gttttatagt caagcatttt ggttgacgat gaacgtgaac
      901 tcgcactcac agactttttt atcatctaca tatgctgcag ataacgctgt accgactgat
      961 cccttcattt tggtgatttc aaaatgacat ttcagcacat ctcctggaac aactttttga
     1021 cggaatttgg ccttattgat cccaccgata taagctcttt ttcctagaaa ctcttctgat
     1081 tttaggataa gtatcgaacc tgcttgtgcc agtgtttcta gaatcaacgc acccggcatc
     1141 actggattgt caggaaagtg accttggaaa aaatcttcat tgattgttac attttttgtc
     1201 gcaacgatct tttttcctgg ttctaaagta tctacataat caatataaca aataggataa
     1261 cgattaggaa tcaattccat tacttcagta gcagataata cgtaagacat aattaaactc
     1321 cttctttcag gcaattgcca ttttcattac tttgactatc aaagtatcat attcctaaat
     1381 atttagcaat ctttttttaa tttaagaaaa aatagtttga caatcaaaac attttgacta
     1441 ataattaaga aatcaagaag ttattttagc tattattctc tattttttta cgactatcaa
     1501 atttttatca gaaacaaagg agagtcatta tgtcatttct agaaggaaaa aaaatcgtca
     1561 ttatgggcgt cgctaacaaa aaaagcattg cttggggttg tgctgaagca atgttgaaac
     1621 aaggggcaga aattatctat acttaccaaa atgaacgaat gaaaaaatca ttagtcaaat
     1681 tggtcggcga agaagcattc actgtagaat gtgatgtttc tagcgacgaa agcatcgaaa
     1741 aagcaatgaa tgccatcggt gaatatgctg gggagatcca cggccttgtc catgcagttg
     1801 cttacgccaa caaagaagaa ttgtccggaa atgtgtctga tatttccaga gacggctatc
     1861 aattagctca agatatcagc agctactctt taattgctgt atcaaaatac gcacaaccgt
     1921 ttcttgcacc aaacagcggg atcgttagca tgacttatct aggttcagaa cgcgcaatcc
     1981 cgaattataa catgatgggt attgcgaaag cttctcttga agctgctatc cgttatttgg
     2041 cagctgagtt ctcgcctaaa ggaattcggg tcaatggaat ctcagctggg gcaatcaaaa
     2101 cattagctgt cacaggtgtt aaagactatc aaaaactgat ccagttatct gaatctcgta
     2161 cgcctgatca gcaaggtgtg acgatcgaag aagtcggtaa tgcttgtgct ttcttaatca
     2221 gcccactttc cagcggaatc atcggtgata ttatctacgt tgacaaaggt gtccatcttt
     2281 cataaacttt tgttgcattc tttaaagtaa aacatataaa aatcaggaaa aaacttttta
     2341 aagtctcttc ctgatttttt ctttttacgt ttttttcaac tcattacttt gaaattattc
     2401 cacgacttca atccagcctt ctggtgcttc tatgtctcca aattgaatac ctgtcaattc
     2461 atcatacaac cgttttgtta ctggtcctac ttccgtttca ctataaaata catgaagatt
     2521 gttattgtga taaatcccac cgattggaga aataacagcc gctgtaccac atgcaccagc
     2581 ttctgcaaat tgatctagat catcaatgaa aatatctcct tcaattgctt gcagtcctag
     2641 tcgttcttca gctaactgca acaaggaata tttcgtgata cttggtaaga tagagggcga
     2701 ttgcggagtg atgaattttc cgtcttttgt aattccaaag aaatttgctg cacctacttc
     2761 ttcgattttc gtatgtgtgg ctggatcaag ataaacacaa tctgagtaat ccagttcttt
     2821 tgcttctgtt cctggaagta aactcgctgc gtaatttccg ccgacttttg ctgcaccagt
     2881 ccccttagga gcagcacgat cgtaatcaga tacaataaaa ttcgttggtt ttagtccacc
     2941 tttgaaatac gctcctactg gcgtacagaa gacgacaaat aaatattctg gagctggatg
     3001 gacaccaatg ttttcaccga ccccaatcag taaagggcgc aagtataatg tagcgcctgt
     3061 tccatagggc ggcacaaaat cttcatttgc ttttaccact tgcttgacag catcaacgaa
     3121 catcttttca gggaccattg gcatcagcaa tcgtctagca ctttgattca tccgtcgact
     3181 attttgttca ggtcgaaaga gattgacctt gccgtctttt cgacgataag cttttagacc
     3241 ttcaaaacat tgctgaccat aatgaagcgc tggagagcct tcatgaatcg tcaggtaatt
     3301 atcttctgtc aattctcctt cttcccattg tccgtctttc catttggcaa taaaacgcca
     3361 aggagttttg atataagaaa aacctagatt gttccaatcg atttgcatat acttaccacc
     3421 ttcgttattt tctccacatt ttatcattat ccatattatt tgtcatttta ttttcagctt
     3481 ttttccttca ttatagttat ttttgctatt tccttttttg aaaacttctt ggtacaatac
     3541 agaaagaatc aaattttaga aaagaggatt ttatgttttc attattagca actgccggta
     3601 cgacagactc ttctggcgag acattgacag atcaagctgt cagcaaagta aatgcgtttc
     3661 agcggttttg ggacgggatt gattgggacg ccattttggc tgcctttatc caaaaaagtt
     3721 tatatctgat ttttttgatc gtcctttttt ggattcttca ccgtgtggga aaatatctga
     3781 ttgataagtc ttttaatcag tattctaaaa aagccattct aaacgagagc cgcttaaaga
     3841 ctttacatag tcttttaaat actatttttc attataccct tggtttcttt tttatctatg
     3901 caattttatc tgctatcggt gtgccgatcg gatcactttt ggctggtgcc ggaattgcag
     3961 gtgtggcaat cggtcttggt gcccaagggt tcatgagtga tgttattacc ggatttttta
     4021 ttatcatgga acaacagatg gatgttgggg actacatcaa gctagcaaac ttaacgattg
     4081 aaggaaccgt cgtatctgtt ggtatccgta cccttcagct gaaagcaacg gatggtactg
     4141 tccatttcat cccgaatcga aacatcacca cgatcagcaa tctctctcgt gcaaatatgc
     4201 aagtactttt agatattcgg attgttcctg aagaaggata tgacaaaatc tatgagatca
     4261 ttgatcgagt aaatcgtaca ttagctgaaa aataccaaga agagctccaa actgaacctt
     4321 ctatctttgg attagtggat cttggaaaca gcaattttgc gattcgtacg gtctgctacg
     4381 cattgaacgg aaaacaatat gccttaaaag aagagttctt gagctcctat gtcaaagaat
     4441 tgacagcttc tggtttcacg attccgaaca gtccaattgc tgtcggaaaa taatttggac
     4501 aaacgatgca acgaatctca ctaaaaagag cgtgggacaa aagtaaagaa cacttttgtc
//
LOCUS       DAYYAG010000170         4559 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000170 DAYYAG010000000
VERSION     DAYYAG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.174"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACHARQ_002725"
     CDS             complement(29..1261)
                     /locus_tag="ACHARQ_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8411103.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACHARQ_002726"
     CDS             1516..2085
                     /locus_tag="ACHARQ_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8411104.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACHARQ_002727"
     CDS             complement(2263..2703)
                     /locus_tag="ACHARQ_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8411105.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACHARQ_002728"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACHARQ_002728"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8411106.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..>4559
                     /locus_tag="ACHARQ_002729"
     CDS             3657..>4559
                     /locus_tag="ACHARQ_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8411107.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTK"
BASE COUNT         1371 a          852 c          906 g         1430 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaa
//
LOCUS       DAYYAG010000171         4483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000171 DAYYAG010000000
VERSION     DAYYAG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.175"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            672..1361
                     /locus_tag="ACHARQ_002730"
     CDS             672..1361
                     /locus_tag="ACHARQ_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8411108.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1348..2556
                     /locus_tag="ACHARQ_002731"
     CDS             1348..2556
                     /locus_tag="ACHARQ_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8411109.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2850..4121
                     /gene="serS"
                     /locus_tag="ACHARQ_002732"
     CDS             2850..4121
                     /gene="serS"
                     /locus_tag="ACHARQ_002732"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8411110.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1445 a          775 c          892 g         1371 t
ORIGIN      
        1 ttgttgtctt gaattgaatc aagacgccct acacatttgg tttgtcttac tttgttcagt
       61 tttcaaaggt ctacttcatc aggagtgttt cccgattcgt ttttggctgc cgtagcaact
      121 tttatatcat atcagagaga caagtaattg tcaactcttt tttaaaagtt ttttcgattt
      181 cgtttttcga aatcttccag caactcattt atcataactt atcgtttgtt atttgtcaag
      241 aacttttttg aagttttttt cgatagaatc aagcttcgca agtggttctt ttttaacgac
      301 tcagttatct tatcatgtca tttccaacat gtcaacaact tattttttat tatttaataa
      361 gttattggtg ttcttttgaa caaataataa tatatcaaca tcacatgtga aagtcaataa
      421 gaaaatgctt atttatcaac atttctagct tacatgatca ttggttagtt ctggtccttt
      481 ttcgttcgtc tttataactc cgagagacgt aaaatccttt tgttttcagt ttaatgttca
      541 tgttcttgtc ttattcacac caactagatg tctcaccttc taaaattttc cttaacaaag
      601 tgctgctttt atctatttct gctttttttt gctagaataa tagtaacaat atgactctgg
      661 aggatttatt tatgaaaatt ttgattgctg acgatgataa agaaatcgtt gaattattaa
      721 gtatctatgt tcacaatgag gggtatgaag cagtaaaggc ttatgatggg aaagaagcat
      781 tatctaaaat cagaacaatc cctgatattg aattacttat tttagatatc atgatgcctg
      841 agatggatgg gatgcaagta gtgaaagagc tacgaaaaga atcccaaata ccaatcatta
      901 tgctgacagc aaaaactact gatatggata agatcaaagg actggtcgct ggtgcagatg
      961 attatgtgac caagccattt aatccgttag aagtaatggc tagagtaaag tctttattac
     1021 ggcgtacaaa aatgcagatt gccccagatc agcctgatgt attagaagta aatgcattaa
     1081 tcatcaataa agactctcat gaagttaaaa caatcgatgg gaaagaaatc cagttaacag
     1141 ctttggaatt cggtatcctc tacttgcttg ctagtcatcc taatcgagta tttagcgcgg
     1201 atgaaatttt tgagcgtgtt tggaaacaag aaagtattgt ctctgctaaa actgtcatgg
     1261 ttcacgttag tcatttaaga gataagattg aagaagcaac tggtggtgaa aaagtcattc
     1321 aaaccgtttg gggagtgggc tataaaattg acgcaagata gtaaacgagc aactgaaaaa
     1381 cgccgtcgga tcaatttgac ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc
     1441 acgattatcc ttttgcttct attaaatgtt tctattctgg ttgttatcag ttcggtgatc
     1501 aacagttcac cttctttgac gaatgcgatt tgggattcaa agaatatttt tgcagaacga
     1561 ttgaacactg atttgttctg gaacggccgg aattttatta ttcccttttt ctttctatta
     1621 gatattggtg ttttgtattg gcgcttgatt cgaagatatc gccaaatgca attgcggcat
     1681 atcatcagcg aacttcacta catcgcaaac ggaaattatg atcatcgtat cccttttgaa
     1741 ctgagtggtg acttaagccg agtggtaaca agtatcaatg gattggtgga tagtaccgta
     1801 gcagcgattg aagacgaacg taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc
     1861 agtcatgata tccgtacccc actcacgtcc atcattggat acctaggact gatagaagat
     1921 ggccagtatc actcagaaga agatctttta aagtatacgc atactgctta tatcaaagca
     1981 aagcagatga aatctttagt ggacgactta tttgaataca ctaaggtacg ccagcctgct
     2041 gtcccggtga atttttctgc ctttgacatg atccagttga tcgaacagtt agcagctgat
     2101 tttgagttgg aagcttctaa aaaaaatatc caaatacttg ttcaatcaaa agtagactcc
     2161 cttatcatgg atggcgatac cgaaaaactt gtacgtgtct tcaataatct tttgacgaat
     2221 gcgttgaaat acggaaaagg agcaacgaag atcgtaatcg aagtagaacg tatcggttca
     2281 gaagttgttg caacagtcaa aaataacgga gcaatgatcc cacaacaggc aatcgataat
     2341 ctattcgacc gtttttatcg tgtagaagaa tctcgttcac aagcaactgg aggaactgga
     2401 cttggtttag caatcgctca aagcattgtg gctttgcacg gcggatacat ttatgcaaaa
     2461 tcagataagc aatggacttc tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta
     2521 cctattgata cacaggaagt tattgacgaa agctgaaata ttcgctattc ttattacaat
     2581 tattagaata aatgaaaaac acagaaccca gtacacttta ttctttttca gagagctgat
     2641 ggcaggtgta aatcagcaaa ggataatttg gaagttccag ttcgtttacg tgccctttcg
     2701 aaataagaaa ggtcggttca acagccgtta tgtgttcaac aagtgcagct gacgctgaac
     2761 ttgggtggta ccgcgaattt cagactgatt cgtcccaaga ataatgggat ggatcagtct
     2821 ttttttattt tcaaaaagga ggaaatcata tgttagatgt aaaaatgatg cgacaaaata
     2881 ttgaagaagt acaagaaaaa ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat
     2941 tcatggaact agatgaaaat cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat
     3001 atcgaaatga tgtttctgct gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg
     3061 ctaaaatcgc tgaaatgaaa gaagtcggtg gcaacatcaa agcattggat actgaaattg
     3121 ctgaagtgga tgaaaaattg aaacaaattg caactacttt gccaaacctt ccccatgatt
     3181 ctgtgccagt tggaacagat gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac
     3241 gtacatttgc ttttgaacca aaaccacact gggaaattgc agaaaatctt gatattttag
     3301 attttgaacg tggcgcaaaa gtagccggta gccgttttgt ctattacaaa ggattaggtg
     3361 ctcgattaga acgtgctgtc tacaacttca tgcttgatca gcatatttac gaacacggct
     3421 atactgaaat gatcactcct tatatcgtca acagtaaagc gatgtttggt acgggacaat
     3481 ttccaaaatt caaagaagat gttttccaat tggagaatac tgatctaaca ttaatcccaa
     3541 cagctgaagt accattaact aactattata atgatgaaat tttagatggt gcagatttac
     3601 ctatttattt cactgcacta agccctgctt ttcgttctga agccggaagt gctggtcggg
     3661 atacacgagg actgattcgt cttcaccaat tcaataaagt agaaatggtc aaattttcgg
     3721 atgtagagca ttcttacgaa gaattggaaa aaatgacagc tgatgcagaa gatatcttga
     3781 aaaaattgca tttgccttat cgtgtgatgg ccttatcaac tggagacatg ggcttttctg
     3841 cagctaaaac ttatgactta gaagtatgga tacctgcaca agatacttat cgtgaaatca
     3901 gttcttgttc taactgtgaa gatttccaag cacgccgcgc aatgatccgt tatcgcgata
     3961 atgatggaaa aatccactat actcatactc tgaatggttc aggacttgca gttggccgta
     4021 cagtagctgc cattttggaa aactaccaaa atgaagatgg ttctgtcaca gttccagaag
     4081 cgctcgttcc ttatatgggg aatctgaaag ttattaaata aagaggctgt gaaaaagccg
     4141 cttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat
     4201 tggacttaat accgagaaac tggcttttga acaccattta tcgagaggtt gtgaaatcag
     4261 cgaattaacc tgagtattag agaaaaaaat ggaaaaatag tccctcatat tttaccgttt
     4321 gttgaactaa taccgagggt cagctgattg aacaccgttt agtggagagg tcgtaagcct
     4381 accccccagc tcctagttac aaggacaaat tcaaaaaaca gctcttcata ttttatgaat
     4441 gttgtacttg tgattcggag gtggcaaggg aacactgttt att
//
LOCUS       DAYYAG010000172         4450 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000172 DAYYAG010000000
VERSION     DAYYAG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.176"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1381
                     /locus_tag="ACHARQ_002733"
     CDS             47..1381
                     /locus_tag="ACHARQ_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8411111.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1422..2333
                     /locus_tag="ACHARQ_002734"
     CDS             1422..2333
                     /locus_tag="ACHARQ_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8411112.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2330..3184
                     /locus_tag="ACHARQ_002735"
     CDS             2330..3184
                     /locus_tag="ACHARQ_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8411113.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3181..3798
                     /locus_tag="ACHARQ_002736"
     CDS             3181..3798
                     /locus_tag="ACHARQ_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8411114.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3800..4318
                     /locus_tag="ACHARQ_002737"
     CDS             3800..4318
                     /locus_tag="ACHARQ_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8411115.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1334 a          758 c          902 g         1456 t
ORIGIN      
        1 ttatattaaa accggcatcg gttacattaa aatttggagg taaaatatga aaaaattatt
       61 gtttacaggg ttgctggcaa gttcagcatt gtttttatta ggaggatgcg gatcaagtaa
      121 tgcaagcgat gccagttcaa gtgcagcaaa ttcttctgaa aaaacaagtg atggaagaaa
      181 aaatattgat ttctggtcct tttggggatc cggtgcacga aaagaagcta tcgaagaaat
      241 cattgaggat ttcaatcaat cacaaaacga gatccatgta aattataagt atcagccatg
      301 gggtgacatc tggactaaat ctttatctgc aataacagct ggaaatgcac ccgatgttat
      361 tgtacaggac ataaattccg ttgcccaaag agcagaagca cagcaagcaa ctaatctctc
      421 caaatatgta gaaaaagaca cttctaagga tttttatccg cagctttggg atacagtaga
      481 atatgaaggt gatccttatg cgatcccatt caatacagat acacaagtca ttttttataa
      541 taaaaagtta tttaaagaag ctggaatcag tgaacaagat ctaccgacaa cttgggatga
      601 attagaagaa acagcacgta aactagataa aaaagaagga aaagaattta cacgcatcgg
      661 tttttatcca ttatggaatt taggggctga tgtatgggcg ctaaacgcag ataacgggac
      721 tagttggttt gacaaagatg aaaaagtcaa gattgatact aaaaataaag tggaagcctt
      781 ggagtggatc caagactggc aagattatta tgggaaaaat acgatcaata aattagaagc
      841 cgaatttggg tctggtgtat ctgatccatt tatttccgga ttagtagcca tgcgtgccca
      901 aaatatcaat tattactcta gtttgatgga aaatgcaccg gatgattttg attttggcgt
      961 gatgcagctc ccagaaaaag aagaaggttc aggccattgg tcgtggggtg gcggattcgt
     1021 cttggaagta ccatacggag caaaagatcc tgaggcctct tatgaattca tcaaatatct
     1081 ttcttctcca gaagtacaag aaaaattcgg cgaaaaaagt tttgatatca tggcgagcaa
     1141 gacagccaat gagaatttag tgaataatga caacctaacg gataaaggaa aaatgatcta
     1201 tcaaatggcg gacgaaaact ttaaaaatac ggtgatcaca ccagttcctt tagatgctcc
     1261 ggattacacg acattagtga atgaacagat tgaccagatt cttttaggaa caaaatcacc
     1321 aaaagaagga ttggcagatg cacaaaaagc agtagaaaac ttagtcaagc aaaatcaata
     1381 atccattttt gactgtctag gtacagagga ggcaagaaaa tttgaagcgt ttatctatcc
     1441 gaaagaaaaa agaagcaaga tgggcctatt tatttatctc gccttttatt atcgggttct
     1501 tggtatttat gctagggcct atgctttttt cagttattgg tagtttcacc gattataatt
     1561 tgacatccaa gatgaatttc attggtctag caaatttcaa aagaatgttc gttcacgatg
     1621 atttattttg gaaatcgtta tacaacaccg tttatttcgt catcttcaat gtaccgctga
     1681 cgacgatttt cagtgtgttt ttagccacag tactcaatca aaaaatcaaa ggaatcagtg
     1741 tattccgtac agctttctat ttaccggcag tcttatcagg agtagcagtc tacatcttat
     1801 ggatgcagct gctttctcca tctgcaggat tgatcaacac atttctgggc tggttccata
     1861 tccctggacc agcttggctg tttgatccac aatggacgaa acctgcgttg attttgatga
     1921 aaatatggag ttctggagga gcaatgctac tttacttagc tacattacaa aatgttccta
     1981 gatctttata tgaatcagca gaaatcgatg gagcaggcgt ttggggaaag ttcaaaagta
     2041 ttactttgcc attaatcact cctattattt tttacgatgt gatcacctct acgatcggtt
     2101 ctttccaaat ttttcaagaa gcttatgtca tgacgaaaaa tggaacaggc ggacctgcaa
     2161 attctttgct tttttataat ctgcatatgt ggaacaaggc gttcgtagct tttgatatgg
     2221 gttatgcgat ggctatgtca atgatcctgt ttctgatcgt gttggttctg acgtttgtca
     2281 atttgaagct tgcacctaag tgggtctatt attctggagg tgacggcaaa tgatggatta
     2341 tttcaaaaag accagtgtga atcggcaaac aaaaaaaata ctaagttatg tcctcatgac
     2401 agtaatagga atcattttga tcattccatt gctttggatg gtgtttactt cattgaaacc
     2461 aatggaagaa atcgttcgtt atcctccgac ctttttccca gaaaaaatcg tatgggaaaa
     2521 ctatctggat acgattgccg cctttccttt ttggcgctat gcaagaaata cattattcat
     2581 cactgtgcta gtagtgatcg gtaatgtcct ttcaaattct tttattgctt atggatttgc
     2641 gaagcttgat tttcctggga aaaagctgat gtttgctttg gttctttcta cgatgatgat
     2701 cccggggttt gtaacgatga tccctcaata tgtattattc tccaagatcg gctgggtagg
     2761 tacttatttg cccctgatcg ttccatcgtt cttcggcaat gcgttcaata tatttctgat
     2821 gcgtcaattt tacttatcga tcaataatga attgatcgaa gctgctgaaa tcgacggagc
     2881 gaatcatttg tatatttgga gtcatttgat gcttccattg acgaaacctg ccttgattac
     2941 cattgcgatc aattccttca acgcagcttg gaatgatttc ttaggtcctc tgttatatat
     3001 ccaagatcaa gaaaaataca cgttacagat tggcttgcaa gtttttcaga accaagcaac
     3061 gacacaatgg aattatctga tggcaggagc aacacttgtc ttgatcccga cgattttgct
     3121 gttctttttt gcacaacgtt atttcattga agggatggat ttgacaggag gttcaaaagg
     3181 atgatgaatc gatcgattgg taaaaaagcc aatgattatt tcttagaaat cattgaatgg
     3241 attccacgtc ttatccattt acagctacta tggttcctgt gcgtcttgcc tgttttcaca
     3301 ttaggaactg caagtcgaac agttctgtat atgatttatc attacaaaaa aaatgaagag
     3361 aaaaagctcg gttcactatt ctggaaaaag tttcgtgaga acttctctct ttatggaaag
     3421 caagatagtt tagcaagtgt ttattttctt cttctattga tcgattatca gattttccgc
     3481 tattggggtg ggggaattgg ctatacattg atgtatacta cgttgttttt gcttctatta
     3541 agcgcgcttc tctttagtta caaaatcttg cttcaactgg aacaaaaaga agcttcttgg
     3601 attactgcat ttttcttgtt ttttaatgat ttcaaaagcg cgcttttcta tttggcaggc
     3661 acgttgatcc ttcttgtcgc attctggttt gcaggaccaa tctattttgc tttattaggt
     3721 ttcagtttct tgttttattt tcaagtcctg ctttttatcc atcgtacgca agaaaaggtt
     3781 cttaaaaagg aggaataaca tgttgcgttt gattgctaaa atcgatgata gtattatcaa
     3841 caagttcgcc aagcaacgga aaacggatag tcgaaaaatc aattttttga aatttatcag
     3901 taatatctct aatggtggtt ttatttggtt gctccttgct gctgttttag tcttgatacc
     3961 tgatactaga gaatatggtc tagccattct tatcggcagt ttgatctatt tttttgttgg
     4021 tttgcttttt atcaaaaaaa cgacaagacg gaaaagacct tatgaaaaaa atcgtcggct
     4081 tgtcctttac acaagtaaac cgaagacttc ttcttttcct tcaggacata cagcgctagc
     4141 ctttttatca gtagctgcgt tgttcgaatt aagtccagtt gcaggaatat tagccttttt
     4201 atttgcttgt ttgatcgctt attcgaggat ttttttactt ttacattatc ctactgacat
     4261 tttaggagga gctattttag gaagtgttat tggcatccta tgcgctgccg ttatctgaat
     4321 taattgtacg aataaagcat gagtgcgtaa atgcgcattt gtgctttatt tttttgtttc
     4381 gaggaaagcg tagttgttta ttcatttgtc ttaataactt aagttttgtt aaatgagaac
     4441 gttataaaat
//
LOCUS       DAYYAG010000173         4347 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000173 DAYYAG010000000
VERSION     DAYYAG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.177"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..585)
                     /locus_tag="ACHARQ_002738"
     CDS             complement(100..585)
                     /locus_tag="ACHARQ_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8411116.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(587..1258)
                     /locus_tag="ACHARQ_002739"
     CDS             complement(587..1258)
                     /locus_tag="ACHARQ_002739"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411117.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1521..2714)
                     /locus_tag="ACHARQ_002740"
     CDS             complement(1521..2714)
                     /locus_tag="ACHARQ_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8411118.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2798..2998)
                     /locus_tag="ACHARQ_002741"
     CDS             complement(2798..2998)
                     /locus_tag="ACHARQ_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8411119.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3422..3655)
                     /locus_tag="ACHARQ_002742"
     CDS             complement(3422..3655)
                     /locus_tag="ACHARQ_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411120.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3662..4093)
                     /locus_tag="ACHARQ_002743"
     CDS             complement(3662..4093)
                     /locus_tag="ACHARQ_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8411121.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1091 a         1072 c          841 g         1343 t
ORIGIN      
        1 tgatgaatta actgataaaa aaatatctac tatttaaaga tacagggata agtaagtaaa
       61 aaaataacat gtcattagat agtagacagt tccaactctt tatttacctt ttttgtatgc
      121 tgcactgtca atccaagttc ctaaattgtt gtatccatca tcacttatat aatcatattg
      181 gccatcaata atttcgtcaa accacaaata ttctccatct ggtttttgcc caattatttt
      241 aattactgat tgactagtag ttgtcatctg gttaaaatct tttattttac ctatattaac
      301 ggccaaaata gcatttcctc gcttataact ctcctggatt tcatatttaa catattctcg
      361 atcaagagtt tctttaccta tcagcaccac agtcactgat gttccaatta attgattatc
      421 tatccagttt tttatagcaa tatctccatt acgtttaact ttctcaaatt cagccttatc
      481 tacaaaacca gcttcctttg ttcctcgcaa agtccagctg tttctaacta ccattgctct
      541 tgttacatct gcataatgga aactaaaaaa aacccttctc accatattaa tcctcctgaa
      601 tactatttat taaactgaca atttgttcaa cttttttatc tacttctcct tcagtatcta
      661 atttttctag cgcttgaaat ttacttttta aggaatcatc aaaataatca ctgaaattct
      721 cttgaatctt atcaaacaaa accttacttg cacctttggt aaatccaatc ggaactacta
      781 tattttcatt ttgaacagat aattcaaatt cttcaatcat tccatctgct gtaactattt
      841 cacctgattc aagtttgttt ccaaaaacaa aaatactaat cccagactct gcaataatgt
      901 tttctcgata tcttctccac tcatgaggca tatcaagggg aagtggtctt aaagaaagta
      961 cgtcttgtat gttatttttt cttaaatttc ttctctgctt tagaactcct gaaacaatac
     1021 tattaccaat tccaagtccg taacctgata ctattctata accggattca gaaagcctat
     1081 atgctaattt ctcgacaaat tttaatcctt tcgattcaga tgtaaatgga gtataatcaa
     1141 cagcacttcc cgaaataaaa atagtttttc tcaaatactt ttttctaata cttttcaaaa
     1201 tatcagtaat ctcagaataa tcatctactt caacaacgtt tatcgaaaat ctggacaaat
     1261 cattgatttg tagtcttctt tttgtcagtt cataattata tctctcttga tcaaaaaaat
     1321 cctcagaatc gacaaaattt tcctttaaaa ttatatggga ttttagatta tctttttaga
     1381 agttcttgaa tgtcggtata gcccgtgttt ccgggctttt ccggcatttt tgatattgtt
     1441 agatacttac aaatgttctt attacctctc accatttccc cctcaaaagt agtaaatgga
     1501 gtagtaaaac cggccttgtg tcaggctgcc agccgttcca tttctgccct tgcggaatcg
     1561 aatgtggcgt gtgcataata gttcagcgtc atggtgatat tggaatgtcc cataatgtac
     1621 tgcaaggctt tggggttcat tcccgcattg gctaaccggg tgcagaatgt atgacgcata
     1681 gcatggggcg tcgtcgtttt cggcaacgct tcttcatggc tcttgttgta cttcttcaca
     1741 agcctgccaa acatcccgcc ataatctacc gccgtcatgg gatagccgtt ctgtttgaga
     1801 aacaggaaat tggcataccc gtcaataatg aacggctttg cacctttgcg gttttttatc
     1861 actcgctgga acgcctgata cacttcctca ttcatgggga tttttctgac gccgctttgg
     1921 gtcttgggtt cgtcaatata gtaaccgacg ccggtatttc tcaaaagctg gtggctcacg
     1981 atgattaccc ggttttcaaa atccaaatct ttgtccgtca gtccgcacag ttccgaaatg
     2041 cgaagccctg tccccagcag aataacgatt gcgtcataat gcttttggta aaccttatca
     2101 ccttgaataa aggacagaaa actttcttcc tgtgctggtg taagaggcac ctttggttcc
     2161 gtatcgtcgt ccagaacgtc actaagctgg aagtcaaacg ggttttttcg gatgcaatcg
     2221 tcctgtatgg cggtgtaaaa agccgctttc agggaacgct tgtcgttatt gatggtctta
     2281 taagaaaggc ccttttcttt catccgaagc gcccattcct ttgcgtcgga cagcttcaca
     2341 ctgtcaatcg ggcagctccc cagccgatct tgttccagaa tacgcatcag ccgttcccgg
     2401 cccagtttgg tgttgtgctt cacattgccc cggtgcctga tctgcttggc gtagagctgg
     2461 cagacggtca ttttcttgcc gatggtgtct atcccgtcgt caaggtcttt ctggatttct
     2521 ttctcctttt cccggagtga aatatcctcc cgtttacctg ccggggtctt atccgtaggc
     2581 actaacttcc atgagtaaac aaactgcggt tttccaaagg tatcaacata tttgtaagca
     2641 tatcgtccgt cttttctctg gctctctcca gaccgcaaaa cgcgattttt gctgtctcgt
     2701 cttttttctg acatctcgtc atgctccttt ccatgacgga aagagccgca tatatgctta
     2761 taaattatta taacatatct gcggcccttt ttcactatta gattgtgtcc aaagtgtcaa
     2821 ttattttctc aaactgcttg cgtttgatct ggatgcggtt gccattcata atgacccagc
     2881 cagaggccgg attttcctcg gccagcttac gcaatttgtt ttctccgata cggaaatatt
     2941 tagacgcttc ctcaatcgtc agcgtgtatt tttcccaaat aggcacatca tggttcataa
     3001 tgcagccccc tttcacaaat tcaaaggttt gtgtgattta ggggcataga cggacggctg
     3061 ctggcacagc tccacgggac tttcaccccc gcgtggttct cacgcggccc tacccattgc
     3121 ctgcgacgct tttaacgctc ggactgtggc tataagggag tatcatagtc ctgcctgtac
     3181 gtcgtcgccc gcaagccgcc acgctcacat tgcggcgtgg tgttcctcgc tccgcttttc
     3241 ccgtagggaa aagtatcatg gcgcaccagc cgtccggctc ggcacagaca gaatgtgtcc
     3301 gtatgcccta tgatgcgccg ccgttatctt gcgggcatat ttatcaaggt gcgatcaggc
     3361 caccgggaaa acaccgaaac ggggaaaggg taagccgttt catgctgccg gggccggttc
     3421 gtcagttcgt gacgatagcg tggatgagct tgatttccag acggcgcttc atggcctcgt
     3481 ccagacacac atgcggatag ccgttttcgt catagagcgt ccgggtacac agcttgttca
     3541 tgtaaccgct gtaatactgc aaaacgcgct ctacggctcc ggcatcccca gcgcgggcgg
     3601 cctcgatcac cgaaaggggc aaaagctcaa tgcccttgta gttcgggttc ttcatccgcg
     3661 tctaacctcc cttcggcatc aacgccttta gctgtttccg cagctccgat aaggtatttg
     3721 tcctgtgccg ctgaacggca ctccgggaca ttcccacaag gccgccgatc tcaccatctg
     3781 ccatatccag aacacagtgc aggattaaaa tactctgctc catgctgggc agcgcggcaa
     3841 aggcttcggc aacaagctca ttgttgatat gtaggtcata gccatgcgac gaaaacgtaa
     3901 agctgtcgct ggggtagtga tctactgtac agagcttgtc caaatctgcc tgtgacaaag
     3961 agcttagtga cacttcacgg tcacggcggc gcttcatttc agccaagtag cttttggcct
     4021 cgttacgcag taccgtcttg caaaaagcat cgaacatgca ccgcgcttca tagtcgcggg
     4081 ggatagcatc catcttctca ccccctttct gtgggggact taggtggttc tctccctttc
     4141 cgctaacaag actttgacaa ggccgccagc tacccgctaa atacctcctt tttgaaaatt
     4201 tctcaaaaaa tttttttggg gacagccggg ccgcaaaaga cgacaaaaat cgcccggcag
     4261 gtcgcaaacc taccgggcga gaaaatgctt tgctatgctg ctgatttaca tggtatagta
     4321 catactcata accggaatag ccctgtg
//
LOCUS       DAYYAG010000174         4334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000174 DAYYAG010000000
VERSION     DAYYAG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.178"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHARQ_002744"
     CDS             127..579
                     /locus_tag="ACHARQ_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411122.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHARQ_002745"
     CDS             593..1282
                     /locus_tag="ACHARQ_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411123.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHARQ_002746"
     CDS             1407..3758
                     /locus_tag="ACHARQ_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8411124.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHARQ_002747"
     CDS             3838..4233
                     /locus_tag="ACHARQ_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411125.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1393 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttat
//
LOCUS       DAYYAG010000175         4277 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000175 DAYYAG010000000
VERSION     DAYYAG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.179"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..923)
                     /locus_tag="ACHARQ_002748"
     CDS             complement(27..923)
                     /locus_tag="ACHARQ_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8411126.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="ACHARQ_002749"
     CDS             complement(916..1596)
                     /locus_tag="ACHARQ_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8411127.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="ACHARQ_002750"
     CDS             complement(1597..2379)
                     /locus_tag="ACHARQ_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8411128.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="ACHARQ_002751"
     CDS             complement(2372..3289)
                     /locus_tag="ACHARQ_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8411129.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3707)
                     /locus_tag="ACHARQ_002752"
     CDS             complement(3453..3707)
                     /locus_tag="ACHARQ_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411130.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1255 a          939 c          750 g         1333 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaag
//
LOCUS       DAYYAG010000176         4238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000176 DAYYAG010000000
VERSION     DAYYAG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.180"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..742
                     /locus_tag="ACHARQ_002753"
     CDS             41..742
                     /locus_tag="ACHARQ_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8411131.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1314..2330)
                     /gene="tsaD"
                     /locus_tag="ACHARQ_002754"
     CDS             complement(1314..2330)
                     /gene="tsaD"
                     /locus_tag="ACHARQ_002754"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8411132.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2327..2791)
                     /gene="rimI"
                     /locus_tag="ACHARQ_002755"
     CDS             complement(2327..2791)
                     /gene="rimI"
                     /locus_tag="ACHARQ_002755"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8411133.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2798..3340)
                     /gene="rimI"
                     /locus_tag="ACHARQ_002756"
     CDS             complement(2798..3340)
                     /gene="rimI"
                     /locus_tag="ACHARQ_002756"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8411134.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3324..4046)
                     /gene="tsaB"
                     /locus_tag="ACHARQ_002757"
     CDS             complement(3324..4046)
                     /gene="tsaB"
                     /locus_tag="ACHARQ_002757"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8411135.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1246 a          911 c          734 g         1347 t
ORIGIN      
        1 tgacaataaa tatagtgaac aataaggaga ggacttttta atgcgtgtta ataaaatcga
       61 ttttcacttt tcaaaactca gatatcaacc tgattttgaa catttctcgg atgaagagtt
      121 ccaacagatc aaggaacata ctgttcttcg ttcttataaa aaaggacaga tccttttcga
      181 cgatggtgat cccagacaac ggttgtatat cttgaccaaa ggactcgttc gtttagaacg
      241 ttatgatgaa agtgcaactt attactacta tgactatgtg aatacaaata acctttttcc
      301 tatgtcagga atgtttggag aagagaccta cacttataca gctcaagcga tgactgatat
      361 tgaaacattc taccttcctg tttcctacta tgaaaaatta gcacgaaaaa atcccgaaca
      421 gttgatctat atgatacaaa aaatttctaa agtagttgaa atacatgaat tgcgtatcca
      481 aatcggattg acctctagcg cctttgaccg ggtaaaacaa aatttattca ttttgaaaga
      541 agaattgggc gtaccaacaa aagatggtca tatcaccatt ccttatccga tcaccttaaa
      601 agaattagca gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa tcaaacaact
      661 acgtgattcc ggactgttag attatgacaa aaagcgtttt actttctgta agacagctac
      721 tactccagct atgagagaat aacgcgaaca aaaataagca gtgaccctga tatttatcag
      781 ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt agatcactgg
      841 gcaagactca aagattttct atgtgtgttt cagttatgta atattcactg tttgcaaaaa
      901 atactgatag attaactaga ggaagcgtct tgtcctcatc cgacctagat tattcgatat
      961 tcctcgacaa taaacgatta atcagtgagg gcaatcagag gaacggataa tgatgaataa
     1021 gctgtaaaaa atagggcttt gacaaaagcg tgtcacagcc cttatttcga ttaaacggtg
     1081 tgtacctgtc aactcttcgg tcacaagtca taatgttcat aaacatgagg agctgttttt
     1141 tgaaattgtt ccttgtgaat cagagttaaa cgacaggctt acgaactctt gattaaacgg
     1201 cgttcaaaag cttgcatacg cggtaataag aacagccagg tcaaaaacat gagaagccgt
     1261 ttttcctggg ctgttcttat tacttgatgc agggcagatt tttcacaacc tctttattca
     1321 gataaaacca agcttggatc agcattcaac tgatagctag caaaatttct tttttgcatt
     1381 tccacatgtg ctgctgctcc aatcattgca gcattatctc cacaaagtcg aagaggggga
     1441 atcactagtt ctacctcagg gagtttagtt gccaaagcat cctgcaatcc ttcacgcaac
     1501 ccttgattgg ccgctacacc tccagctaca atcagttgct tgactggata attttgacaa
     1561 gctcgaagtg ttttagtaat caatacatcg atcacacttg cttgaaagct agctgctaaa
     1621 tcatttttgt ctagatcttc tccacgctgt tgtgcattat gcaccaaatt gataaatgca
     1681 ctttttaatc cgctaaaact gaaatcataa ttatcttcat ggatcatcgc tcgtggaaaa
     1741 tggtaattat cttttccctg atgagctagc tgatcgattt cttttccgct tgggtaagaa
     1801 agacctaata ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc
     1861 ccaatgattt catatgatcc atcttcttgc atatagacta attctgtatg tcctccgcta
     1921 acaagaagtg ccatcaatgg aaactggaat ggtttaacca gacgggctgc atagatatgc
     1981 cctgccatat ggtttacagg aataagcggc aattggtgtg cccaagcaaa agccttagct
     2041 gcagaaatac caatcagcaa cgagccgaca agtcctggtc cataagtgac agcgactgcc
     2101 gataaatctt ctgccgacac accagcttct accagtgcat cttccaagca taaggtgatt
     2161 tgttctacgt gatggcggct agctacttca ggaactacgc caccaaaacg tttatgactg
     2221 tttatttgag aagcaacgat gttagataag atctctgttc catttctaac gaccgctaca
     2281 ctcgtttcat cacaacttga ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct
     2341 ccgtttttaa ctttctgctc ataatgatcg catcttccgt cggattgcga taatatgatt
     2401 tccgtttacc taatatacga aacttttctg attcataaag ttttcttgct gcttcattag
     2461 atagacgtac ttctaaaaaa acatgcagcg aatcttttat ttgttccgta atcagtaatt
     2521 gtaacaaatg ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca atatttgtga
     2581 tctcgacttc atctattact ttcgagtatc caataaagcc acagatctgg ttatcttcaa
     2641 ctaaaagcag ataatcagag tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc
     2701 aaggtgaacc atgtggataa gcattttcgc taatcatcca tacgcattct gccaagtacg
     2761 cttcatcaaa atcttttttt ctgcaaatca actaatctca ctcattttct aaaagtttta
     2821 tcatatccaa cgcatcttct tttgtttcag tgtaatatct ttttttgatc gttgttgctt
     2881 gaaaacctaa cttccgataa agtctttgtg catcttgatt actcatacgc acttctaatg
     2941 acatcgtctc acagcgattc attatagcaa agttctcgat ttcgtcaata agcagcgaac
     3001 cgattcgttt acgctgatat gccggtcgaa cggcaatatt cgtgatatgg caatctaaac
     3061 cgaacgctct tgaaccgata aaaccaatca ttcttccatt atcaaagata cctagatata
     3121 gatgaggaag cggtgacttc aattctgata taaaggcact ttttgtccat ggcaattcgc
     3181 cattatagac ttctcgttcg acatccagca gctctttgat atcttctgta gtcaattctc
     3241 ttagtacgta acgacgatcg atttgttcga ttgttttttc tgtataaatc attttatctt
     3301 gaaaaaagct tctggcatac gctctaaatt ttttcaacat aactttcgtc tccaggtgtg
     3361 tgcgtctcca accatttttc ttctgcttct acccgtttta aatagcgagg aagaaaagct
     3421 tgtttctctg caactggttc agctaaagca cctaattgag ccacagttac accacttgga
     3481 atatcccatg ctggaactgt attaatcatc gcactaggca atatttcctt tatttcatag
     3541 aagaatttgt gacaatcact cccgacgaaa taaatagctt cattcagctt ttgtagttgt
     3601 gtgaacaaat cagaagcaga aatatgtaca tcttcaagta caatttctaa tctattttct
     3661 ttccagcggt atgccccagc gtatacattt tttcttcgag catcaaacat gggcacaata
     3721 attttatcca caccgatgca attagccgcg atggttttca gactagatac tcctactagc
     3781 tctttatcta atgtataagc aatcgttttt gctgtcgtta cacctatacg aagccctgta
     3841 taagaaccgg gaccatcaga aaccgcgatt cggtcaatgt cttttggact tatttgaaga
     3901 tctgcaaaca attggtcgat tgcaggcatc agtgttacac tgtggttgcg tttgatattt
     3961 gtttgtattt gtccaagaat ttgttcgtct tctaccacac cgatcgccaa agtttggttt
     4021 gcagtatcta ttcctaatgt aatcatatat ttgatccttt ctgctatgac attctcgcct
     4081 tccattgtag catacttttt tattctgagg atgaaaatac caaaggcaaa actagtacaa
     4141 tgatacatga ttatctgctg gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg
     4201 cggtatcact tcatttcccc acagcttttc catctttt
//
LOCUS       DAYYAG010000177         4234 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000177 DAYYAG010000000
VERSION     DAYYAG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.181"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..291
                     /gene="relB"
                     /locus_tag="ACHARQ_002758"
     CDS             70..291
                     /gene="relB"
                     /locus_tag="ACHARQ_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8411136.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            293..559
                     /locus_tag="ACHARQ_002759"
     CDS             293..559
                     /locus_tag="ACHARQ_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8411137.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            726..1211
                     /locus_tag="ACHARQ_002760"
     CDS             726..1211
                     /locus_tag="ACHARQ_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411138.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVDTKVTKIYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            1417..1599
                     /locus_tag="ACHARQ_002761"
     CDS             1417..1599
                     /locus_tag="ACHARQ_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8411139.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            1702..1986
                     /locus_tag="ACHARQ_002762"
     CDS             1702..1986
                     /locus_tag="ACHARQ_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411140.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            1993..2238
                     /locus_tag="ACHARQ_002763"
     CDS             1993..2238
                     /locus_tag="ACHARQ_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411141.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            2260..2514
                     /locus_tag="ACHARQ_002764"
     CDS             2260..2514
                     /locus_tag="ACHARQ_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411142.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            2596..2781
                     /locus_tag="ACHARQ_002765"
     CDS             2596..2781
                     /locus_tag="ACHARQ_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFM8411143.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            2848..3243
                     /locus_tag="ACHARQ_002766"
     CDS             2848..3243
                     /locus_tag="ACHARQ_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM8411144.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            3260..3610
                     /locus_tag="ACHARQ_002767"
     CDS             3260..3610
                     /locus_tag="ACHARQ_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411145.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            3632..4006
                     /locus_tag="ACHARQ_002768"
     CDS             3632..4006
                     /locus_tag="ACHARQ_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411146.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1523 a          702 c          757 g         1252 t
ORIGIN      
        1 tcaaatatat aactattgca tttgtcatac aatcgtagta caatgtaatt gaaaaggagt
       61 tgataaacta tgccagtaat aagtattcgt gtatctaaag aagaaaaaga ttttcttgaa
      121 aaagttgctg aattcaacgg agataacatt tctgaattta tgcgtagtaa ttccctgcaa
      181 acagcagaaa cgttagtaga ctttgccacc tacaagcaac taatgaaaga gcatgaggaa
      241 aacgatcaaa gtatttccca cgaagatatg ctaaaagagt taggcttata agatgactta
      301 ccaagtaaga tatgaacgtg aagctcaaaa agcgctaaaa aaaatggata agtttcaggc
      361 aaaggttatc ctaaactgga tcgagaaaaa tcttgtaggc actgatgatc caagaaagca
      421 tggtaaaggt ctaacagcta ataaatcagg ctactggcga tatagggtag gtgcatatcg
      481 tataatagct gatattagcg agcatgaagt gacaattctt atattgtcta ttggtcacag
      541 aagtgaagtt tataagtaac agacaagctg ataaggcttg tctgtttttt tgttaaccca
      601 acaaaatcac tgtttacgat cccactttac ctaaaaaaga acaatatcct cttgatatta
      661 tacacaccgg tgtgtataat ataagtgtaa acaagctaat taactttcaa aaggagcgat
      721 tttttatggg atggtatggt gattattcat cagcaaatga agtgatcgaa gaaaagaaag
      781 ggtacgccaa aaatggtttg gaaatcgtag ataccaaagt aacgaagatc tatggaatta
      841 tccttttcaa agatgcagaa ggaacctacg atattgacag cttcatcttt cgagatgaaa
      901 tgtacaaacc aatctacttc ttaaatgaca gtcctgctta ctttaagaaa attccgaaaa
      961 aatggttgaa attctttgtc ggtgatcaag aaaaaatggt taagcgacaa ctagatagaa
     1021 ttaaagaaga aagcaataaa ccaaaacttg atgacttatt agttgcaggt aaaacttact
     1081 caatctggga tggatcgtat gtcgctgtct atcagtataa actcaaacgt agctttattt
     1141 ttcgactaca agatggatca ctcactcggt tcactcgact atcagcagga gatgttaagg
     1201 aagtagccta aaacttaaat acataaacat aaacgtatac acgtttatgt ttatgtatat
     1261 gtatatgaat ataaacgtat taggtacgaa cgcttttaaa ttcttttctg ggctttgttt
     1321 ttagggtagg gctaaaaaca tcaccatctg atccaaaaca gctcagaaac gcatttaaac
     1381 gtatctgata ccaatattac agaaggagtg tatcgtatga ttaacctatt tcttttagtg
     1441 acagctagta cattattctt tactggaata tcactgataa tttcctattt gatctataag
     1501 aaagccgaaa atcacacaag caaagcaacc ggactagccg tttcatcctt tttaactttg
     1561 ctgactttat ttgttacttt atcgttttct tttgtttaga acagctgatc cattagcacc
     1621 tttttctctt tacattatac acaccgatgt gtataatgta tttgtaaaca agctaattaa
     1681 cttttaggaa ggagtgacaa gatgctaaca gctactttac agcaacctac tcacgttgct
     1741 attgcagaaa gttatacaat taatgcaggg atcgttaaac agctgatcaa ggatcgagaa
     1801 ttcaaaaagt ttaacagtcg gcaagtgtat aacagacagc taaagaaagc tattctaaag
     1861 cacaaagatt ccttcatcgg gaacttggaa agtgtagatg ttaaaactgt ttcaacggca
     1921 ttttcagttg ttgaagtgac tattgacggt cgcttgattt ctctatttgc ttttggaatt
     1981 aattgatagg gagtgtggac tttggaaggt aagacttttg cagaaatttg gactgaattt
     2041 attgaagtac caactcgcat tgatccgata acttctgaaa aggtattgga tgcagacttc
     2101 tacaatctca aagcaggtac tagccttgaa tgcgtttgga tgtatttaga agaacatttc
     2161 aaaggcgtaa cgctcggtca cgtcctatac cacaatggtg ccgagtggga tgcaattgga
     2221 aatatccatt tttcttagta caagaaagga gaacacaata tgaattatac cgatgaagaa
     2281 caaatcactt tagaatatgt tttcatagat gaagaagggg aggaaattga aaaagtcttg
     2341 attatttcat caaaagactt ggagaacgct ttgacttatg aaccatacga atcaaccgta
     2401 actgtcaatg aattcttaga tacctatact tctgatgata cttgggaaat catgaatatt
     2461 tgcaacgcta ataacattca atttgctgaa aaaaccaata aatcatttta ttagttttgc
     2521 aataaaacat cctctttttc tattgattcc atacacaaca atgtgtataa tagattttgt
     2581 aaagggaggg tagctatgcc taagacagca agagaaattg taaaaattct caaatctaac
     2641 ggcttcaaaa agactggaca aactggcagt catgcaaaat tttataattc caagactaaa
     2701 agaacggtcg ttgttccgat ccacaatggt gatgtaccac taggaacaga agctaatatc
     2761 tggaagcaag cagggcttta acagccttgc cttcctattt ggtattaggc atactatcct
     2821 ctcttacact agaaaaggag cgaggaaatg aagacaaaaa atatttttta tccggcaatc
     2881 ttcgagaaag aaggagaagc ttataacatc gttttccctg acttaccgga aatatctacc
     2941 tttggaaata gtcttgaaga agcatattta aacgctaagg atgctttagg attagcgcta
     3001 tatagtgtac cagatgcaga atttcctaaa ccatcagcag tggaagaatt aactttgaaa
     3061 gccaaccaag tggtagtaat catccaacta aacattaaac ttttccgaag aagcttaaat
     3121 acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt tagttctttt agggaaggag
     3181 aaaaacataa acttctctca actacttcaa aaagcactgg aagaagagtt gcttgaaaag
     3241 taaaaaggag accgaaccta tgaaagctac acacttaaac tatttctctc gaacgttaat
     3301 caataatcta ttaaaagata caactggcat tagcgttagt accaaacact ttgccaatga
     3361 tctaattatt tttgaggata ataacttgcg aattgagatg tttattgaac aggcaacccc
     3421 taactcaata cctgaattct gctatacgca cactgaaaaa atgaaacatg caaaatcagt
     3481 taaagatgtt gaagcattaa gtgaatcact aacgaacgct ttaaacctcg ttaagaaggc
     3541 aaaattgtta tttgaatttc aaaccgacta ccacaatgag ttgcttgctc aatctgaaat
     3601 tttagattaa ttgaaacagg agaagagaaa gatgaaacaa acacaaacag aaaccgttaa
     3661 cgatacgctt gctgatcaat tagcaggtta ttataaatta tttgagatgg gtaaagcaca
     3721 gcgcttgatc cactatcttt ggaaatacaa aattttgttt cttttttggg aatcaacact
     3781 aacattctca gttctttttt ataagtcaga tttttataat caacacttag ttttagggag
     3841 ttttttgctt atctacacca ttttgatgct taatttatat acctacatca cacttgaaga
     3901 acacttagta attagcgatg cagggatcga gagaaacatc aaaagggtat ccttcaagat
     3961 cacgttaaac aagcctaccg ataaaaagct ctcagaagcg ttctgagagc ttttgacatt
     4021 ttcttgtata attttatcct aacacattac aaagcaccta attcgagctt atatcaagca
     4081 aatttgctat ttattactca atacatatct aaaaattact tgatggaaaa ttagatacat
     4141 ggtaagcatg aaaagaaaat caatttgagc agatgcagta caatacatag caatttaaaa
     4201 gctctcagaa ggcttctgag agcttttaat atat
//
LOCUS       DAYYAG010000178         4075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000178 DAYYAG010000000
VERSION     DAYYAG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.182"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(184..1689)
                     /locus_tag="ACHARQ_002769"
     CDS             complement(184..1689)
                     /locus_tag="ACHARQ_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8411147.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1686..2372)
                     /locus_tag="ACHARQ_002770"
     CDS             complement(1686..2372)
                     /locus_tag="ACHARQ_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8411148.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2565..3986)
                     /gene="gndA"
                     /locus_tag="ACHARQ_002771"
     CDS             complement(2565..3986)
                     /gene="gndA"
                     /locus_tag="ACHARQ_002771"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8411149.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1121 a          859 c          696 g         1399 t
ORIGIN      
        1 gaatattaca gcattttatt gttttttcgt tcatctaaaa gatacttaca tttaattaac
       61 ggatgttatt ctatttaatt gttcgctagt ttttttataa tgacatccct gaatttctca
      121 cttgcttgta ttttgtacct tattctttgt tggatatata aaaaaacaag taaagcacat
      181 gctttacttg tcttctgcct ttgcgatagg aataaagatt ctaaaaattg tcccttgccc
      241 tactacactt tctgctaaga tacgtccttt atagttttca accagttgtt tagcgataga
      301 caaccccaga ccatttccac ccttcgttct agctcttgct ttatccactc gataaaaacg
      361 attgaagatc ttattaaggt cttctttggg tattccttcg ccaaagtcct gaatcgcgat
      421 ctcaaattca ttcagagtgg aagaaataga gatatggact tcttttcgtg tagttgaata
      481 ctttactgcg ttatccaata tgataatgat taattgttca aagtgattac gataaatttg
      541 cagggtcact tcttttgtca aatcatcatc taaagtgatc acatagtctg gatataatat
      601 cttgaaatta ttataaactt gataggtgac ttcttttcct ttagttgttt cattcgcata
      661 atgaacatct acttgttctg ctcttgataa gtctagcatc tcttgaacta agcttttcat
      721 acggctgatt tcttgcatgc tggcgcttaa tgattcttct aatatctcag gatcatcttt
      781 cccccaacga tttagcagac taagatgccc ttcaataatt gcaacaggtg tccttaactc
      841 atgagatacg tcttccacga attgctcttg ttgttcaata tacaaacgca tacggtcaag
      901 catctcattg aatatttcag ctaaatctgc caattcatca tttgtgtcga tttcaggcat
      961 gtggatatct gattgcggat ctttacgtat agtatccatt gtatctctga gtaccttgag
     1021 tggttttaag aaataagaag ataaaaagaa acctaaaata ctacttaaga tcaaagaaac
     1081 aacttctaaa acaataagag tcaataatag cttatttcga atatcataga aagaagataa
     1141 ttcatagaag ctttgcgcat agccgatctt ttctcttgta cctttagaat atataggctg
     1201 gatagataaa aagccagttt ttccttctag tgtaacgatc gttggaactt tacgtgtagt
     1261 ctgaatcagg tttcgactgt tttcatgggt tttaaaaatc aagttctcgt ccaaatcata
     1321 aacataaaga tccattgatg gctgccctag ctcagctata aagggatcga tcttcattaa
     1381 gtttccttcg accagtgaag tatgatctac gacctcatct ccgtcatttg tcgctttcgt
     1441 cagattacga tacgtattta ctaatgtcag ttcttcatcc gagttagcca aacgagaagc
     1501 tacttctgaa atcgtacgct ctacattgtt tctttctttt gccacaatca gattgataga
     1561 tgatttatag gtaatgacag cgaaaattgt aaacacaacg aatatgaaga aagaactcgc
     1621 aaaggcccac ttgattgtca aggagggccc tttcagttct cttttttccg attttttcgg
     1681 ctttatcacg agcgcatcac ataacctgtt ccgcgtacag tttggatata gctttcctcc
     1741 ccgggaacgt caattttgtt gcgaagataa cgaatatata cgtccactac gtttgtttca
     1801 acttctgttt cgtatcccca cactttattt agtaaaacgt cacgtgctaa taccacgttc
     1861 acgttttcca ttaaagtaag cagcaactcg tattctcttt ttgttagttc aattacttca
     1921 tttccgcgac gtacgacacg gttttctttt tcgatagtca aatcgcgata agtaatagta
     1981 gtttgtttag ccacattttt atcgccttca atatcgattc gacgcaacaa tgcacgaaga
     2041 cgtgctaata attcttcgat agcaaatggt ttgacgatgt agtcatctgc tccgtggtca
     2101 agtccagata cacgatcgat cacagaatct cttgcagtca tcataatgat tggtgtattt
     2161 ttgacttggc gtacacgtct gcagacttct aaaccgttca attctgggag catcagatcc
     2221 aacaatatgg catcccactc attatttaat gccgcatcta aaccagttcg gccgttgtag
     2281 tggacttctg cgttataccc ttcatgcttc aactctagct cgacaaatcg tgccaagttc
     2341 ttttcatctt caatgattag aatattgctc atttattatt ttatcctttc tattctactg
     2401 gctttttaaa aactctatca ttataccgat aatatgctta aaaatctata gtcattttat
     2461 catggatagc aattttaaaa aagagctgaa gtctactcat aacaatcgac ttcagctctt
     2521 tcgtcctctt tttattttct catttcctaa cagccgaaag actattattc ttcatggtac
     2581 catgagtaat ggaagattcc ttctttgtcg acacgctcat aggtatgtgc gccaaagtaa
     2641 tcgcgttggg cttggattat atttgcaggc agacgttctg ctctgtatga atcgaagtaa
     2701 gcgatggctg atgagaaagt cggtacaggt acgccagctt ggacagccaa ggcaacgacg
     2761 tctctgactg attgctgata ttttttcgtg atttcaacga agtaatcatc tagcaataaa
     2821 ttttcgatct caggattttt gtcataggca tccgtaattt tttgcaagaa tcgagctcgg
     2881 atgatacatc cagcgcgcca gatttttgcg atttcaccaa atggcaagtc ccaatcaaat
     2941 tcttttgaag cgacacgtaa ttgagcaaat ccttgggcat aactcatgat tttactgaaa
     3001 tacaacgctt ctcggatttt ttcgaccaat tcttttttgt ctcccgtgaa ctcaaaatcg
     3061 ttcgtacgag acaaaatctt gcttgcttgt acgcgttctt ctttataagc agaaatatag
     3121 cgggcaaata cagattcagt gatcaatgga agtggcacac ctaagtcaag cgcgctttgg
     3181 cttgtccatt tacctgttcc tttattccca gctgcatcaa ggatcacatc tactactggt
     3241 ttacctgttc cttcatcgtc tttacgtgtc agaatgtcag cagtgatttc tatcaaatag
     3301 ctgtctaatt cgccttgatt ccattcttta aagatatcag ccatttcttc tactgaaagg
     3361 tttaaaatat tcttcattaa gtcataagat tcagcaatca gttgcatatc tccatactca
     3421 ataccgttgt gtaccatttt cacataatgt ccggctccat ttgggccaat atatgtgaca
     3481 catggtgctc cgtcttctgc ttttgcagaa atttgctcta agattggtgc aactagcgca
     3541 taggcttctt tttgtccacc aggcatgatt gaaggtcctt ttaaagctcc ttcttcacca
     3601 ccggaaacgc ctgtaccgat aaagttgatg ccagagttgg ctaactcttc attacgacgc
     3661 atcgtatctt taaagaatgt atttcctccg tcaatcaaaa tatcgccttt atcaaggtga
     3721 ggaagtaact cttggattgt tgcatctgtt gccggtcctg ctttgaccat tagcaagatc
     3781 cgacgtggtt tctcgataga ttctacaaat tcttcgattg tatatgttgc tttgaaattt
     3841 ttatctgggt gttcttctac tacagcggtt gttttagccc ctgtacgatt gaataaagcc
     3901 actgaatagc cgcggctttc tatgttcaag gctagatttt tacccataac ggccataccg
     3961 acaacgccga tttgttgttt tgacatatga actcctccta catacgggat ttctccctaa
     4021 tctcttacat tatagcttaa cacggctaca ttgcaaacta aagaacaaca tattt
//
LOCUS       DAYYAG010000179         4070 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000179 DAYYAG010000000
VERSION     DAYYAG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.183"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHARQ_002772"
     CDS             85..1434
                     /locus_tag="ACHARQ_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8411150.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHARQ_002773"
     CDS             complement(1562..2152)
                     /locus_tag="ACHARQ_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8411151.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHARQ_002774"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHARQ_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8411152.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1175 a          745 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaac
//
LOCUS       DAYYAG010000180         4067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000180 DAYYAG010000000
VERSION     DAYYAG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.184"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACHARQ_002775"
     CDS             complement(<1..1157)
                     /locus_tag="ACHARQ_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8411153.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACHARQ_002776"
     CDS             complement(1160..3409)
                     /locus_tag="ACHARQ_002776"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8411154.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACHARQ_002777"
     CDS             complement(3506..4009)
                     /locus_tag="ACHARQ_002777"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411155.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DAYYAG010000181         3989 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000181 DAYYAG010000000
VERSION     DAYYAG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.185"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..355
                     /locus_tag="ACHARQ_002778"
     CDS             5..355
                     /locus_tag="ACHARQ_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8411156.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            336..1052
                     /locus_tag="ACHARQ_002779"
     CDS             336..1052
                     /locus_tag="ACHARQ_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8411157.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            1052..2500
                     /locus_tag="ACHARQ_002780"
     CDS             1052..2500
                     /locus_tag="ACHARQ_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8411158.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2570..3079)
                     /locus_tag="ACHARQ_002781"
     CDS             complement(2570..3079)
                     /locus_tag="ACHARQ_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411159.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(3069..3794)
                     /locus_tag="ACHARQ_002782"
     CDS             complement(3069..3794)
                     /locus_tag="ACHARQ_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411160.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1402 a          671 c          814 g         1102 t
ORIGIN      
        1 aagaatgatt tttttcagta ttggtatctt attcttaata attggcagtc tttttcggat
       61 tctgccctca aaaggaaatc tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac
      121 agacgctcat tggcggctgg ctcaaaagac aagcggaaac tggttttttc ttatgggctt
      181 actgatggca ttgatcggct attatttaaa gacatctggg catacgaatt attttctgat
      241 tgaaatgctt ctgctagtat ttccaattat gcctattttt attatgacag aaaagaaatt
      301 acagaaatat gacctggaaa ctggagggaa cgataatgaa tattttaatg attgaagata
      361 atcaatctgt ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt
      421 tcaagtatga tggaaaagaa gggctcgatg cctttttaga aaacccacaa cattgggata
      481 tgattacact agacctgaat ttgcccacac tggacggtat gctagtagga agagagatcc
      541 gcaaagtttc aagtactgtg ccgattatca tgctgacagc tagagattct gaaagcgatc
      601 aggtgatcgg cttagagatg ggggcagatg attatgtcac aaaacctttc agtcctctga
      661 cgcttattgc tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa gcaagagaag
      721 aagcagcagt ttcagatgat cattttgata tcgagactag tcatttcaag atgaatacga
      781 aaacacgaga agcgtatctg gacaaccggc caattgaagg actgacgcct aaagagttcg
      841 atctattgtt tactttagca aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg
      901 aattagtctg ggattatcag tactttggtg atgaacggac agtggacgcc catatcaaga
      961 aattacgaca aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt tggggagtcg
     1021 gctacaaatt cgatgattct ggtgttgcct aatgcgttat ttgtatcagc aactcttagc
     1081 tttttgcgga atggtcgcgt tgatcttatt gatcgttgga gtttctttta cacaactgac
     1141 aaaacaaacg attgaagaaa acaattacca gcaactgttt ggatatgctg aatcagttga
     1201 aaagacaacg caaacctatg ccgatacgct cccgcagttt agtcaagatc aggcgtttca
     1261 aaatgcattg ctgctgacag agcaagtact gacagaacaa aatgtgaact ttatatttat
     1321 cgataaaaac gaacgtgtca tttatccgac taatacagct ttactgaatt ttaccttaac
     1381 agctgatcaa tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa
     1441 tattttagga caagcacagg cgacctctta cgcacttgtg ccatttaatt tgaatcatga
     1501 attctatgga gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc
     1561 aattacactg aacctgttca aaggatttat tttttccagt atcgttgcag ttattgccag
     1621 ctatgctttt gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga
     1681 agtgacgaat ggtaattttg atgtacagct ccccgtacat gacaaagatg aatttgatga
     1741 gttagcagat gatttcaata aaatgacgaa ttcattgaaa gaatctcaag cagaaattga
     1801 agaacaagaa aatcgtcgaa gacaatttat ggcagatgct tctcacgaga tgagaacacc
     1861 tcttacgaca atcaacggac ttctggaagg gttagaatac aatgcaatcc ctgaaaatca
     1921 gcgagaaaac gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa
     1981 tgaaaacttg gattatgaaa aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt
     2041 caatggaaca gaaacattga aaaatattgt tgcacagtta gaagctaaag cagaagcagc
     2101 aggagataca ttgacattga aagccgttga tgatattgat gtttacgctg attatgaccg
     2161 atttgtccaa atcatggtca atattattca aaacgcgata cagtttactg aaaacggcca
     2221 aatcacgatt actttagaaa aaggctatct tgaaacgatc atcacgattg aagatacggg
     2281 aattggaatg tctgaacaac aaatgaaaag tatctgggat cgttattata aagtagatcc
     2341 ttcaagaaaa aatacaaaat atggtgaatc aggattaggt ttaccaatcg tgcagcaact
     2401 tgtacgcttg cataaaggaa aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt
     2461 cgctgttcgt ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga
     2521 tcatgaatcg atcttctata ccgcttgttt ttactataaa atgattgatt tagcccgcgg
     2581 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     2641 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     2701 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     2761 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     2821 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     2881 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     2941 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     3001 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     3061 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa
     3121 gtgaaaggga atagactaag aatgagacag catccactga gcccaataag agtttgggca
     3181 aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc
     3241 tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc
     3301 agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac
     3361 tggaagctga cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc
     3421 tgccagaaaa taaaaaggaa taatttggtt ttagaaaaac cagtcaggcg ccaattgatc
     3481 aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat
     3541 agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga
     3601 agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct
     3661 aaagccgatt gcgaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt
     3721 gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag
     3781 cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt
     3841 atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga
     3901 ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat
     3961 tcgaaaagag gagcagcgac aataatttt
//
LOCUS       DAYYAG010000182         3910 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000182 DAYYAG010000000
VERSION     DAYYAG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.186"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..884)
                     /locus_tag="ACHARQ_002783"
     CDS             complement(<1..884)
                     /locus_tag="ACHARQ_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8411161.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGF"
     gene            complement(899..2620)
                     /locus_tag="ACHARQ_002784"
     CDS             complement(899..2620)
                     /locus_tag="ACHARQ_002784"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8411162.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            2720..3481
                     /locus_tag="ACHARQ_002785"
     CDS             2720..3481
                     /locus_tag="ACHARQ_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8411163.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         1282 a          677 c          754 g         1197 t
ORIGIN      
        1 aagaagccga tcgtcacacc acctgataga acaagcatag caccgaatac cgcacttatg
       61 atgaaggcga gttgattaat gaaacgaatg gccggataga tagcacagtt cataaattga
      121 gcacgtttaa aagcgttgta ttgcttttta tttacttcgt gtatagcagc ttctgcatga
      181 gcttgctgat tgaatgtttt gaccactaga ttaccagcta agatttcttc cattcgatta
      241 tttaaaacac ccagttcata ctgattctgt tcagaaatct gtttgttttt gttcgcaatc
      301 ttagttgtaa caattgaact gccaacgatc agcaataaga cgagcaaagt aagcttgctt
      361 tcgatataaa acagcatcag accagcagca gctattgtta cgacagaagt aaaaaattga
      421 ttgatacctg tcaataacac ttgagaaagt tgattgagac cggtagttgt ccgactaatg
      481 atgtcaccta cttgatgatt gtcaaaaaac gacatcggta atgttttgaa tttttttgtg
      541 atttcttttc gcaccctcaa tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga
      601 ataaatgaag tgatgctgct taagactgaa cagccgatca gcaataagac agggaacaac
      661 aatgcttgtt tgatatcact gaaagaaagc tgtccaatac ctgtttttct tatttctgat
      721 aagagatcat cgatcccgat tcccatgatc aagggcatgg ctacgactaa taggttcccg
      781 aataaactgc atagaaacaa tccgtaaaaa tatttatgtt caggtttgat cattttccaa
      841 aaacgtatca aagcacgcgt gttcaacgtt tttttctttt ccatcgtatc ccttctttct
      901 atattccttg tgattttgca aactcctgat aagtttggca attagacagt agttcttcat
      961 gagtaccttg tccaacgatt tttccttcat ctaaaacaat gatgttatct gcttgttgga
     1021 ttgtactgag gcgttgagca acaatcacga gagttttctc cgacatgtgt ttatgaaggg
     1081 ctgctcttaa tgctgcatct gttttatagt ctaaagcgga gaaactatca tcgaatacat
     1141 agatctctgc tggtttgatc aacgctcgag caatacatat ccgctgtttc tggccccctg
     1201 aataattaga accgccttga gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta
     1261 gttcttctaa ttgagcgatc ttgatcgctt tagacaactc ttctgttgag gcatattcat
     1321 ttcccatctg caagttgctt aaaattgtcc cgctgaataa gaaagccttc tgagggacat
     1381 aactgatgtg ggagcggatc tgttcttgag tcatttgagt aagaggaata ccaccaaggg
     1441 ttacttttcc tatcgtaggg tcactaagtc tcaagagaag tttcgccaaa ctactttttc
     1501 cagatccggt cccgccaacg atcgctgttg tttttccttt aggtattgtg aaatcaatat
     1561 cttctagaac tggatcagag atttcagtat atttaaatgt gacatgttca aaagcagctg
     1621 catttgtctg atcaaaggtt ggttcagcta ctgttttgat cgtctcttcc tcaatttcga
     1681 tctctgcttg cagtatttct tctatccgtc gtaatgaggc aaacgattta gggataatga
     1741 ccaataccat agctgcgatc atcaagtaag caagtgtcag catagagtat tcgactacag
     1801 cagtcacgac accgatttgt aatgctcctt ctgctacaag atttccgcca aaccacaaaa
     1861 tagaagcata aacgattccc atgagcaaaa aagcaattgg agtgacccaa gcaaatattc
     1921 ggttcacttt gatcatttga ttagcatagt cagcaaagct cttattcgtt cgcttttctt
     1981 catatgattg attatcaaag gcacggatca tattaatccc agtaaagaat tctctcaatg
     2041 tgatggtgat ccgatccatt tttggctgga tactcaaaga taaaggtgcc cctcgtttca
     2101 tcagtcgata aacaatccat acataaaaaa caacagagat caatgtaacc catgttagta
     2161 atagagaata acgaaacatc atgagaatgg tgaaaacgca aataaccggt gctggtaaga
     2221 tcatttggca aaataaaaca atcatttgct ggacattatc gacatcattc gtcattcttg
     2281 tcaaaagaga tccagaacta aaacgactga tatccttcat tgacagctgt tggaatttac
     2341 ggaaatattt ctcccgcgtt tggaatccaa acctagcgga agccaatgct gaaaagtaac
     2401 tagaggcaac tgcagcgatt gttcctagaa acgctacaag cagcatttca actccaattt
     2461 tttttataac tgaggcttcg ccacttgcta tacctgtgtc gatcagcttt gctactaatt
     2521 ttggtacacc taatgtaccg gcgatttcta aacagatcat cacaaaggta aggagagcga
     2581 gtcctttgtt tttctgtaag aatgttttta agatagacat attattcctc cttttttcct
     2641 gatacttttc gattctatac cttcaagtaa cttgaaggtc aatagaaaaa agaataaact
     2701 gaaaaagagg tgagaaaaaa tgaaaaaaat gtatacgatt ggccaagtat cggaactatt
     2761 tgatttgcca aaatcaacga tccgctattg ggatgaacaa ggattaatcc gttcaagtag
     2821 acaagaagag aatgattatc gattgtttga tatcgatgat atttttatgc tttatgatat
     2881 cgttttttat agaaaattag atataccgat caaacagatg aagaatttat atggaaaaac
     2941 attgacggag ttatatgaga cacttgatga aacagaaaga agaatccata aagaactggt
     3001 ggtgatgaaa caaaaacaaa aagagataag ggagcaaaaa aaacagttga aattgatgat
     3061 cgatacaaac gaggaagaat ttcctgtaga agagatccct tttgactgta tgatctcaac
     3121 ggagtttgaa gatattgtgg aaatcaaaaa atttctacct aactattcaa gctttgggat
     3181 gatgagtatg ccagcttcca gttcacagac gatgtatgga ttttttatcg accctagtga
     3241 agtacattta tttactcaag acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg
     3301 attcttattg aaatccgaga tgaaccatgc ggaacgtaat aatattttag aaatcagaga
     3361 aagaatgtat gaaaaaggat ggaaaacagg agaagtgatc ggccagtatc tattgacgaa
     3421 tacggatgaa aacaatatca gaactgaata ttatcatgca tggatagaaa tgaaaaaata
     3481 ggaaatatgt gtttaagata actcgaaaat gatggttgtt ttagttataa agaaaaattt
     3541 tctggttcga ttacgggttt aatgtggggg attattagtc gtggatagat agaattttag
     3601 ttttttagag gaaatatgtc tttaaaaatg gaatggttgc aattcttagc gatatatacg
     3661 atgtaattgt cctcttaaaa aagtatggaa aaataattgt attgggtgtt atgtataatt
     3721 catggtgaac tgagactaat gagaagaaaa attcatattg ttatgagtga gattgtatca
     3781 gatgagaatg ataaattaaa taggtgcata aaagcattac acttaaaata cattgttgct
     3841 tacattatta caatatgtta ataaggcaaa aataaataga tggattgaat aaacatattt
     3901 tttaatcaaa
//
LOCUS       DAYYAG010000183         3884 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000183 DAYYAG010000000
VERSION     DAYYAG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.187"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHARQ_002786"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHARQ_002786"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8411164.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHARQ_002787"
     CDS             complement(2260..2511)
                     /locus_tag="ACHARQ_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8411165.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHARQ_002788"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHARQ_002788"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8411166.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHARQ_002789"
     CDS             3461..3679
                     /locus_tag="ACHARQ_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411167.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1079 a          824 c          718 g         1263 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaa
//
LOCUS       DAYYAG010000184         3881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000184 DAYYAG010000000
VERSION     DAYYAG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.188"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1001
                     /gene="pplA"
                     /locus_tag="ACHARQ_002790"
     CDS             66..1001
                     /gene="pplA"
                     /locus_tag="ACHARQ_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8411168.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1091..2194
                     /locus_tag="ACHARQ_002791"
     CDS             1091..2194
                     /locus_tag="ACHARQ_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8411169.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2207..3160
                     /gene="menA"
                     /locus_tag="ACHARQ_002792"
     CDS             2207..3160
                     /gene="menA"
                     /locus_tag="ACHARQ_002792"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8411170.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT         1284 a          696 c          777 g         1124 t
ORIGIN      
        1 attagaatgt ataatgaatc acaaagaaaa attgtggact agaaatggaa tggtggggta
       61 ttaaaatgaa gaaaaaacgt tttgtaactg gatttgccgt attagcattt tcagctctag
      121 tgttaggcgc ttgcggcgca gatgacaacg gaagctcaaa ctcttcttct gaatcatcta
      181 cagcacaatc ttcgactgca aaaagcacaa cagaatcatc tgcaaaagtt gtagctggcg
      241 gagatttgca agacggcaca tataaattag aagaaaaaaa ttattcaaat ggttaccgtg
      301 cagtctttga aatggttgta aaagacggca aaatcactga atcaaaatat gataacgtca
      361 atgaaaacgg cgaatcaaaa acaaaagatg cagaatacaa caaaaacatg gaagcaaaat
      421 ctggcacaaa cccagaaaaa tacattcctg agttgaatga ccaattctta aaagcacaaa
      481 gcgcaagcgg tgtggaagtc gtaactggag ctactcactc atctgaaagc ttccaaaact
      541 acgcacaaca attgatccaa gctgctcaag ctggtaacac agatacaatc gaaatcgaca
      601 atggtgctga tttgaaagac ggcacttaca agcttgaaga aaagaactac tctaacggct
      661 accgtgtgca attcgaaatg acagttgctg gcggcaaagt aactgaatca aactttgatt
      721 atatcgacaa agacggtaaa tcaaaacaag atgatacaga atataatgaa aatatgaaag
      781 caaaaagcgg tacagaacca aaaacttaca tccctactct gaacgacgaa ttcgtcaaag
      841 caatgggcga agaagatggc tcacctgcta atgtagaagt tgttacaggt gctacacaca
      901 gctcacattc attcatcatg tacgcacaac aattagtgaa tgcagctgaa aaaggcgaca
      961 ctcaaacaat cgaagtagat aatatcgtaa cagaaaaata atcacataat catactaata
     1021 aaagttggag ggtgggacga tagccagatt aggcagtcgt ctcacctttc aaaatgaaga
     1081 gaggaaaaag atgcggaaaa aactactatt ttccttaatc acactgggca tctcttttat
     1141 ggtgctaact ggctgcagca actcagcaaa taaaacaacc actgaaagtt cagcagctac
     1201 caagatattg aaagatccat attctgatga acagttcttg ttagggactt acgttcgtat
     1261 ccgtgtgtat gatgaaggca aagaatcagc attgaaacct gccttcgatc gagtaaaaga
     1321 acttggtgat aaaatcacga tcaatcaaaa aggttctgag atcgatgaag tgaatgagca
     1381 agctgggatc aaaccagtaa aagtttctga tgacgtctac acgttagtca agcgggccta
     1441 cgaatatagc caagattctc aaggcggatt cgatatggcg attggtgcaa tcacacaatt
     1501 atggcgaatc ggttttgatg acgccagaaa accttcccaa gaagaaatcg atcaggcatt
     1561 gaaattagtg gattatcata agatcgaact aaatgacaaa gaaaaaactg tttatctaaa
     1621 agaaaaagga atgatcatcg atttaggtgc aatcgccaaa ggttacatta cggacgaagt
     1681 agtgaaagtc ttgaaaaaac aaggagtgac cactgccatc gttgacttgg gcggtaatgt
     1741 ctatgtctta ggccacagcc caagaggcga aaaccaagac tggacagtcg gcatccaaga
     1801 tccgaacatg gctcgcggat ctgttttagg ttctattaaa gaacggaaca aaacattagt
     1861 tacctctgga atttacgagc gttatttaga agtagatggc aaaaaatacc atcatttgtt
     1921 tgatccaaaa acgggttatc cgttcgacaa tgatatcgca agtgtcacga tcataacaga
     1981 taaatcaatt gatggtgacg ggttgtctac agcagtcttt tcaatgggtg tgaaaaaagg
     2041 actggaatac gtcgaaagcg aattgaataa cgggacggaa gcgatcttcg tgacaaaaga
     2101 cgataaagta tatgtgaccg atccaatcaa agacaccttc aaattatcgg aggactccca
     2161 ttacacgatg ggtgaccgtt ctgaactaaa ataaggagta cgaaccatga cactaaaaat
     2221 ctttttagaa gtagttgaaa tacgaacaaa ggtggctagt gtatttcctt ttgcggtagg
     2281 ggtattattc tctatcgctt tttttcatga gatacattgg ctaaacactt ttttattttt
     2341 tattggaatg ctagtttttg atttagcgac taccgctatc aataattaca tggattatga
     2401 aaaggctcat tctgaagtat ataagtatga agaaaatgtc attggccgca gaaatctatc
     2461 tcctgtattg attcgaaaca tgattttagc tatgattgca tttgtttttg ttatcggcgt
     2521 tattctaacg atcaatacgg gttggctttt acttttgatg ggatttatct gttgttttat
     2581 tggagtattc tatacattcg gaccgattcc tttatctcga atgcctttag gggaaatttt
     2641 cagtggggtg actatgggcc ttggaatttt tgccatggtc gtttatgtca atacattcgg
     2701 ttcagatgtc tatgatctga cagtcaactt ttcatctgga caatttgctt tggtaggaaa
     2761 tctttgggct atagcagcaa tcgtattagc ttccttaccc ttggtgttta cgattgccaa
     2821 tatcatgttg gctaataatt tgcgggatct tgaccgtgat attgaaaatc atcgctatac
     2881 actagttttt tatatcggac gtcctgcggg aacagtctta ttccagctat tgatgtatgc
     2941 ttgctatttc gtgattttaa tggggttgtt gagcggtatc tatgaatggc cgatcttggt
     3001 caccttcttg accttgccaa ttgtgaagaa gcatttggat caattcaagc aaacgttacc
     3061 ccagcctatc agtttcagct attccatcaa aaatatggtc ttattcaatg ccagttatgt
     3121 attggggcta ttactaacaa tcattatgca agccatttaa cagcatgaag aggttgtgaa
     3181 aaaactgccc tgcatcaagt aataagaaca gccaaataaa aatagctcct tatatttttc
     3241 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttatc aggaggttgt
     3301 gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt
     3361 ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta tcagaggttg
     3421 taaaatcagt gttttgtcct gagtattaga gaaacaaatg gaaaaatagt tcctcatatt
     3481 ttgccgtttt ttgagctaat accgaaggac agctgattga acactatttg ttcggaaaaa
     3541 gtgactaact atgacacaat ttttttcaca gtcccttttt tcacttgatt tgattcgtac
     3601 cagcgaactt gacgagaact ttcttggttt ggtaactata tggtattctt ttaacatcct
     3661 cgtttgcttc ttctaattta tttatacaaa gtcacacttt tttaaacatc tgttcttcga
     3721 tacatttcta tttcttttaa acatcgatac caatattttg aacttaagag tgcataccaa
     3781 atggattcat ttactcttcg tcagttacga agataatcaa aatctccaac ttccctactt
     3841 atttcgtttt gagaattatc gaagcaatct tacataaata a
//
LOCUS       DAYYAG010000185         3878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000185 DAYYAG010000000
VERSION     DAYYAG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.189"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACHARQ_002793"
     CDS             complement(718..3867)
                     /locus_tag="ACHARQ_002793"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8411171.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYQQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          802 c          637 g         1363 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgttgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAYYAG010000186         3873 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000186 DAYYAG010000000
VERSION     DAYYAG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.190"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1065
                     /locus_tag="ACHARQ_002794"
     CDS             142..1065
                     /locus_tag="ACHARQ_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8411172.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1071..2324
                     /locus_tag="ACHARQ_002795"
     CDS             1071..2324
                     /locus_tag="ACHARQ_002795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8411173.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2458..3804
                     /gene="gor"
                     /locus_tag="ACHARQ_002796"
     CDS             2458..3804
                     /gene="gor"
                     /locus_tag="ACHARQ_002796"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8411174.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1326 a          623 c          851 g         1073 t
ORIGIN      
        1 tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa ataattttca
       61 aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc tactattatg
      121 aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac tattgaacaa
      181 gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga gcaatttgga
      241 ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga attagcacac
      301 aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga aaaaatgaaa
      361 aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat cacagttggt
      421 gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc gtatatttat
      481 gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa aaaaggcgca
      541 gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat ggaagaaaaa
      601 ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca atccaaacgt
      661 ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga tcgtgaaaga
      721 ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta tgaagatagt
      781 catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt gaatgaactt
      841 tatcagcatc attactgtac atggaaattg gacggattat ttactccagg cgaaaaattc
      901 gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg tagctgggat
      961 caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga aaaccgtgaa
     1021 ttagatacag gattctttta tattgatcct tctgctatca aataaggagt atgatgaaag
     1081 aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca ggaacactgg
     1141 aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc gggaatgcat
     1201 atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa ggagttgctt
     1261 ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc catgaaggaa
     1321 atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt tctgcagtga
     1381 ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt ctgccgatcc
     1441 atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg aaagaagaag
     1501 ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca gagattcgta
     1561 aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc tcttattctg
     1621 gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc ggatgttctc
     1681 aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa cgaaacagtt
     1741 taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat atgtcaatga
     1801 tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc gaaggaagaa
     1861 tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct gtagacagtt
     1921 atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag ttatggaaag
     1981 tagcacaacg agaattagct acaggttttt actacaatac accaactgaa aacgaacaat
     2041 tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta ttatcttatg
     2101 atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta ggagatgaaa
     2161 tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt atgcataatg
     2221 aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact atgccagtcg
     2281 cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa agctgatata
     2341 tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact tgttttctgt
     2401 agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag gaacaaaatg
     2461 caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc agcaaacaga
     2521 gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg cggaacatgt
     2581 gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat tttggaaaca
     2641 attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt tgatttcgcc
     2701 ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta tcaaagagga
     2761 ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga tagtcaaacg
     2821 atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac tggaggccgt
     2881 gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg attttttgaa
     2941 ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc ggctgaactt
     3001 tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa agaacgacca
     3061 cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca agaagctgga
     3121 ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga cgaatatacg
     3181 gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc cggtggacgt
     3241 gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga caaaaaaggg
     3301 tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc tgttggtgat
     3361 gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg tttatcagaa
     3421 cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc gacagtcgtc
     3481 tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt ggaaaaatat
     3541 ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt tgcattaaat
     3601 gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga aagaatcgtc
     3661 ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc cgtggctatc
     3721 aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc aacaggagcg
     3781 gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt tttattgaac
     3841 aaaccatgta aaaccagaaa atcagtatac tga
//
LOCUS       DAYYAG010000187         3838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000187 DAYYAG010000000
VERSION     DAYYAG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.191"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..711)
                     /locus_tag="ACHARQ_002797"
                     /pseudo
     CDS             complement(97..711)
                     /locus_tag="ACHARQ_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            951..1196
                     /locus_tag="ACHARQ_002798"
     CDS             951..1196
                     /locus_tag="ACHARQ_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411175.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1294..1515
                     /locus_tag="ACHARQ_002799"
     CDS             1294..1515
                     /locus_tag="ACHARQ_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411176.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            1837..2517
                     /locus_tag="ACHARQ_002800"
                     /pseudo
     CDS             1837..2517
                     /locus_tag="ACHARQ_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            2657..3196
                     /locus_tag="ACHARQ_002801"
     CDS             2657..3196
                     /locus_tag="ACHARQ_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411177.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT         1358 a          567 c          681 g         1232 t
ORIGIN      
        1 ttaacataaa tgtaatcaac ttagctagat gaatctaaaa tgattgtctg tattgatgac
       61 aaagctaatt aataattacg tgattcaaaa aagaaatcat acaattagtt ggtcgatttc
      121 tttttttatt cggtaaattg tttgcctagt cacattgtag tctgttgcaa tctttgagat
      181 agcaacccca ttctcaagat cctgaacgat actcctatag acaaggcgac gttgaggatc
      241 tttggtgtct gcactgtata acttaggacg acctttatag acgccgtttg ctttagcgat
      301 tttaattcct tgtgcctggc gtcgtttcga ttcagtacgt tcttgttcag caatcatggc
      361 tgagatttaa atgattaagt ctttgatgaa tttatctagg agaggatttc caattgcttc
      421 agccatgatc ggtaaactgg tgataataag tcgcacattc ttcttcttta gataattcac
      481 tgaatcgata atctcgtcat aattgcgtcc aagtcgatca atggcttcta caataaaaat
      541 atcttgctct ctgacaaaat ctaaagcttc ctggaaaatc ggccgatgac taatcgtcgc
      601 tccagattgt ttttcgataa aaattttttc agcgccaaac tttttcattt cttcaatttg
      661 acgttgttca ttttgatcga tggatgaaac tctaatataa gcaattttca aatttattcc
      721 ctacttttct ataacgttta taatacaccc taatcatacg ttagaactct aaatttttca
      781 atgttatatt tttgtctatt tagggtaaac tctaaataga caaacggttt ttgaattcta
      841 aaagttaaat agtttataat agaagaaaaa gaatggggtg acgatttctt agattgaaaa
      901 ggaaagataa atattataga ttattacttt tgacagctga agcaggatgg atggattttc
      961 cagaagttgt aaaagaagtt caagagatag gtgcaactat ttctgatagc cgctcctctc
     1021 ctatgaacta tcaggatgcc catggaatta aaataattga tcaagagaga aatgtcagaa
     1081 aatacacaac aataaatgag tgtgtaatta atgaaaatct ttgtagggca ccgataacga
     1141 aacacattaa gaatagaagc aaagcaaaag acggaagaac ttttactacc ttctaagttt
     1201 ttttcatatt tgtttgctat acttagatct aaggaattag actttcgctg cccattattt
     1261 aagtttgagg tagcgtcttg cgagggatca ataatggaag aagaaaaaat atttgaaaaa
     1321 cgctgggaat tggcttcagt tgaacaaaga gcaaggtacc ataatctaat gtcctcttac
     1381 cgtaatattg attggactta caaagaaaag aagtacttgc tttggttatg tcagttagat
     1441 gtaaatacct ttgaaacatt tgaagtgatt ctagataaga ttaaaaacag caatgaaaaa
     1501 agagctgatc tataatggtt cgctcttttt attttattaa ctctttggtt actatatgta
     1561 aaatacagct tcatattaca ataaatttca ggatacaaat ttatttaagc atatataaat
     1621 ataattgaaa taggacataa atatatgagt atgagaaagg tgattccatg tttaataaat
     1681 gggcaaacca cccaatgaac tcgagaggta gagataattt gaaggagacg gacaagttct
     1741 gggaagaagt atatagtaaa aacggttctg ttgcaaagtt ttccaaaaaa tctattttag
     1801 tgtaaaattg agaaaaaaga cagagaggac agagtaatga atcattttaa aggcaaacaa
     1861 ttcaaaaaag acgtcattat tgtcgctgtt ggttactacc tgcgttacaa tctaagctat
     1921 cgtgaagttc aggaattgtt atatgatcgt ggaataaatg tttgtcatac tacgatttat
     1981 cgttgggtat aagagtacag caaagtcctc tattatcttt ggaagaagaa aaatagacaa
     2041 tccttctatt catggaaaat ggacgaaacc tatatcaaaa ttaagggacg ttggcattat
     2101 ctttatcgtg caattgatgc ggacggctta accttagata tctggttacg aaagaaacgg
     2161 gatacgcaag cagcctatgc tttcttaaaa cgactccata aacagtttgg tgagccgaaa
     2221 gcaattgtga ccgataaagc accttctctt ggctccgcct ttagaaagtt acagagtgtg
     2281 ggtttatata ctaagacaga gcaccgaact gtgaagtatc ttaacaattt aatagaacaa
     2341 gaccatcgac ctattaaacg acgaaataaa ttttatcaaa gtctccgtac agcctcttcc
     2401 acgattaagg gcatggagac cattcgagga atatataaaa agaaccgaag aaatggaacg
     2461 ctcttcggct tttcggtgtc tactgaaatc aaggtattaa tgggaataac agcctaagat
     2521 atttggagtt cagagagggc gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt
     2581 gggttttcaa gtgctttttg gttcattaat cttaaccata gtgtttttaa agtggaaaac
     2641 aagcaacaaa aaaagtatgt ggagccttgt aggaatagtt cttattattt ttactgtgac
     2701 tgcagcaaca gaagcaaatt attcgcataa aattcagtcg tataaaggtc gtttagttat
     2761 gaccaaaaaa gatgttacac gttatacatc ctcaagaact ggaggcctat atgactatgc
     2821 accattagat gcattgaaat ataaagatca tttaaagaaa catgaagtaa aagttggtga
     2881 tcggtgggag aatgcatctc ataaagcata taattccaaa atagtctata cagtagaaga
     2941 tgccgatgag caaaatatca cattacctgt attcaattat tggggaacag ttgtgaaagt
     3001 aaatgggaaa aagagcacta tagaaaatga caatggttta gtgtctatta aaggtacggg
     3061 gaaaagaact atagttgagg tttcatctac gtatccaact acgatgattc tatctttatt
     3121 aattagttca ataactttta tattcttatt tacacgttat ttaataagat gctgcggtga
     3181 aatgaaaata gattagatag atattataaa tatgttgatt ctattttcat agcaatggag
     3241 aaaatacatt agcaatatat aaaagaaggt atcaagagtc acagtgaatt tattccgaaa
     3301 agtgaaaaga gcaaatataa aatttaatga aaatatagaa ctaaatagga aaacgtagac
     3361 gtaattttct acgttttcct tttcatatat attaaatatt acggaaaatt atataaacta
     3421 atttagtgtt ttgtttgtaa ttcctaattt tgctccactt ttagataatt tttaggtcta
     3481 tttaactcat cagacattct atgctcattt ttctccaaat ttgtttcaac tctttcgtac
     3541 ttaattttgc gtatttgaaa attcttcttt tagagtactt ttattacgat actagtatga
     3601 tatatatcta tagcaaacag ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac
     3661 ctataaaatt tttagatgaa gcgacaagtg gacaattaat aaactacttg aacatgaaat
     3721 tatggatcac ctttcaaatt tagaaaatgg aattcttata cttattgcgc aacatttatt
     3781 gattgcaaaa ttatgtgaca aaaaaaacta gtagaagaaa gaataatggc ttgataat
//
LOCUS       DAYYAG010000188         3813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000188 DAYYAG010000000
VERSION     DAYYAG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.192"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..912
                     /locus_tag="ACHARQ_002802"
     CDS             94..912
                     /locus_tag="ACHARQ_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8411178.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            925..1212
                     /locus_tag="ACHARQ_002803"
     CDS             925..1212
                     /locus_tag="ACHARQ_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411179.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(1394..1747)
                     /locus_tag="ACHARQ_002804"
     CDS             complement(1394..1747)
                     /locus_tag="ACHARQ_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411180.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1767..2822)
                     /locus_tag="ACHARQ_002805"
     CDS             complement(1767..2822)
                     /locus_tag="ACHARQ_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411181.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            3038..3436
                     /locus_tag="ACHARQ_002806"
     CDS             3038..3436
                     /locus_tag="ACHARQ_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411182.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            3439..3612
                     /locus_tag="ACHARQ_002807"
     CDS             3439..3612
                     /locus_tag="ACHARQ_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411183.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         1276 a          551 c          688 g         1298 t
ORIGIN      
        1 taaatataaa cacgtttatg tatataaaca tatacataaa cgtgtttata gtgtataatg
       61 gagttacaaa aggttaataa gggaggttta atcatggaaa agaagttgtc aatcattact
      121 ggaaacattg aaaaaggtgg aacaggtaaa acaacaacaa tctacaatct aggtggatat
      181 gctgtcgaaa atttaggtat gaaggtgctg tatattgatg aagataagtc gcaaaaccta
      241 tcaaaacgat ttcctgatgc tttcagtaaa gcgaaaagag agaatacaat caatcattta
      301 tatcttcatg gatcagctga acctttagct ataaatgaga acatggatat tttagtagca
      361 ggtgaagatt taaaacaagt tgagatgcaa atgcgtgata tgcctaacaa cagaacccta
      421 ctgtttagat ggatcgttgt taactatgaa cagcttaaag agaagtatga tctaattctg
      481 attgatactc ataatgatgc aggtctacta gctcaaaatg cttgggctgt atctgatctt
      541 gtcgtaggca tttctgatcc atctaaagat ggttttgaag ctttgctgaa gcttggtaaa
      601 gatattgatg cactagcaaa acagttgatt gatattaaga ctggtgaaac actgatggaa
      661 actaaatacg ctacgatcgg tagcaggatc aaacataata ctaattcaag tcgtgagttc
      721 ctagaattgg taccaacgtt atctaattat cttggatatg tacaagagaa ggaattgctt
      781 aatgcaacta gcttagaagg taagaatatt gctgagttcg ctaaggataa gaatatctat
      841 cgtaagaata aggatttctt tgaaagcacg tttacgctat acaacaaaat aattgatacg
      901 gcacaagaat aaggagtgat tgaagtgaaa gaaaatagat ttaaaaaatt cgctgaagct
      961 gaagtagaag aagttcctga agtggttgaa gcagttgaag aacctaaaca aacacctaaa
     1021 gaaacaacaa gttctttggt agctaaacta aaaagtgata gttctgaaaa gaagaaaaat
     1081 tatcaattca cgcttcaacc atcacaaaga aagaaattat ctaaaatcag cgaaaagtta
     1141 ggaattcgat cagattcagc tttacttggt gaaatgatag ataacttgta tgatgaagtt
     1201 tttggtgagt gatcagttta aagctgatca ctttttttat tggaataagc ggttaggtat
     1261 agcttaacta aatttcgtat acgtgtttat ggatatatac gtatacatgt ttatatacat
     1321 gtatacgtat atgtttatgt ttgtatttgt acataataaa aaggctaggt attgtaacct
     1381 cgccttttta tttttaaagt aagtctttgt tacttggtgc attgaaccct tttagcattg
     1441 gcttttcttt ctttgctgaa tgatcggatg taaccgttga aggatgctct tgttgaatag
     1501 ggttatgatc agttggtttc ttctttactt ctgctacttt gctatcgttt ctatcgttgc
     1561 ctgcttcaat aattgtaact tcattgttct ttacacgccg atataggttc aaagcttttt
     1621 tgatcgtctg agagtattta acgcctagtg tttcatattc ggattcaaga aactctgcta
     1681 aaggtaactc gctaaccttt tctctattga ttataactac ataacgctct gattttccct
     1741 tattcatgtt gatctccttc gctgttttat tgagttaaac caaacttgta aaagcctttt
     1801 acattagaaa attcactgtc ttcaacaaac aaggtatgcg gaattttatc ttttacaagt
     1861 tgtttgtcga taatagaagc tccaccacca gtaaagacaa ctttatctaa ggttgtgaag
     1921 tttttgaatg tagctaaaac ttctgcaaca accatattgg tgtatgtttt tatttctctt
     1981 tcaactactt ctgaaatatc aaaactttca tttccgcttg gcttaaatag atatacgcct
     2041 ttttctttgc cttctcggat aatcttttca agttggaaag cattaggact gatcggaagg
     2101 tcaagcatat ttacgatacg attatataaa gtaaaaatgc cggtatcctt ttgttcacga
     2161 ttgttttcgt caatttcaaa attttggaca acatcaaaaa gtttcgtgcc accaccaata
     2221 tcaacaaaac cgatcttctg actgatcaat gtttcatctt gtacactacc atcatcagca
     2281 agtaggaggt tatatagtgt tccaatcggc tgtggcagta cccaaacggt atctacgttt
     2341 atattataag ccttaccgtc aacttcaact gaatgttgtc gtgtggcttg ttgcatcacg
     2401 tatttgacga ttgtttcggt atagtcggat gaaggcagac ctaaaactag attaacggtt
     2461 aaaacgtcat cagattgatc ttctaaacgt gctaggttag ctaaagagaa agataggata
     2521 tttttgtagg tttcctgctt gtatctgctg atcccaaaac ctagtgattc tatcacgtag
     2581 tcatcttttc ctagctcgtg tatatccttc ccaaaataat agctttcagt agtgtttact
     2641 aggctatatt cagaaacgtt tagtgatcgt tttaatgagg aacctatatt agaaaaacgt
     2701 cctgaattaa tgatattttt gttcaaaata cgagagggaa gtgagagtgg agaaacgtgt
     2761 gtactcattt cttttactga tttattccct aagtcaacgg aaaacaataa tggttctttc
     2821 atggtatgac ctctttctgt ttgtgtttat ggtatttcgg tacgaaaata gtttacacct
     2881 atttactatt agttgcaact gtttataaga aaaaaatgca atcataaaag tctaattaat
     2941 tataataaag tgtaaataaa aaggtgtaaa taaatgcttg caaatttatc ttattatgat
     3001 attataaaat cataaattat attaaggagt gatcctgatg cgattgagaa gaaataagaa
     3061 ttacactacc attattttag aagattttac tgatcaagaa ttacttattg tcggtaaaac
     3121 aaaagcagat tttatcgaaa agggaaatgc ttcaatagta aaagcttcaa aggttgctgt
     3181 cgaaaaatat tttgattctt tagaagaaag tgctaaggaa atttttaatg gagagaaagc
     3241 tggtagctat gagttagacg gtattagatt tagcttgatc ccaacaactg aaaatattat
     3301 tccgaagaaa ttatattata tcttttcatc tgaaaaacat acttcaatga agccttgtat
     3361 ttttgattgg caatggttga ttgatgctgt attggatgta aaatgttcaa aatacaatat
     3421 tactttgggg tgttaattat gagtatgcta gttagaaaat taccattatc tgtaagagaa
     3481 tttctatttt acttggtctg cttaatcgca tcagggttta agctgatcga acatattttt
     3541 atctttgctt atgtattagc aatgatcgca tttttatctg gcggttggtt aattatttat
     3601 ttgttaagct aagttttaga tcaaaaataa ttagtaaatt aaacgataaa ataaccgtta
     3661 ataaatgtaa tgaaaggaca acgcctaaca atcagcttgt aataagatta taaaataaca
     3721 taatatcaaa aatagtgctt tttaaaggac aaattaagtg tatcaacata aacgtgtata
     3781 tgtatatgtt tatgttgata ctttttttgt ctt
//
LOCUS       DAYYAG010000189         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000189 DAYYAG010000000
VERSION     DAYYAG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.194"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHARQ_002808"
     CDS             complement(<1..353)
                     /locus_tag="ACHARQ_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411184.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHARQ_002809"
     CDS             complement(613..1161)
                     /locus_tag="ACHARQ_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411185.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHARQ_002810"
     CDS             complement(1162..2019)
                     /locus_tag="ACHARQ_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8411186.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHARQ_002811"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHARQ_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHARQ_002812"
     CDS             complement(2581..2910)
                     /locus_tag="ACHARQ_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8411187.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHARQ_002813"
     CDS             complement(2987..3259)
                     /locus_tag="ACHARQ_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8411188.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHARQ_002814"
     CDS             complement(3259..3522)
                     /locus_tag="ACHARQ_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8411189.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYAG010000190         3717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000190 DAYYAG010000000
VERSION     DAYYAG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.195"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHARQ_002815"
     CDS             72..155
                     /locus_tag="ACHARQ_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8411190.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHARQ_002816"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHARQ_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8411191.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHARQ_002817"
     CDS             complement(1280..2143)
                     /locus_tag="ACHARQ_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8411192.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHARQ_002818"
     CDS             complement(2145..2417)
                     /locus_tag="ACHARQ_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8411193.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHARQ_002819"
     CDS             complement(2434..2643)
                     /locus_tag="ACHARQ_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8411194.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHARQ_002820"
     CDS             complement(2721..3617)
                     /locus_tag="ACHARQ_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8411195.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAYYAG010000191         3671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000191 DAYYAG010000000
VERSION     DAYYAG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.196"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHARQ_002821"
     CDS             64..972
                     /locus_tag="ACHARQ_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8411196.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACHARQ_002822"
     CDS             972..1592
                     /locus_tag="ACHARQ_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411197.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="ACHARQ_002823"
     CDS             1596..2045
                     /locus_tag="ACHARQ_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411198.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..2539
                     /locus_tag="ACHARQ_002824"
     CDS             2045..2539
                     /locus_tag="ACHARQ_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411199.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2553..2984
                     /locus_tag="ACHARQ_002825"
     CDS             2553..2984
                     /locus_tag="ACHARQ_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411200.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2986..3123
                     /locus_tag="ACHARQ_002826"
     CDS             2986..3123
                     /locus_tag="ACHARQ_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8411201.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            3160..3453
                     /locus_tag="ACHARQ_002827"
     CDS             3160..3453
                     /locus_tag="ACHARQ_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411202.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3450..>3671
                     /locus_tag="ACHARQ_002828"
     CDS             3450..>3671
                     /locus_tag="ACHARQ_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8411203.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT         1320 a          546 c          813 g          992 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     2521 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     2581 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     2641 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     2701 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     2761 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     2821 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     2881 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2941 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     3001 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     3061 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     3121 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     3181 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     3241 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     3301 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     3361 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     3421 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     3481 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     3541 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     3601 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     3661 gaggaaacaa a
//
LOCUS       DAYYAG010000192         3566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000192 DAYYAG010000000
VERSION     DAYYAG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.197"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..172
                     /locus_tag="ACHARQ_002829"
     CDS             <1..172
                     /locus_tag="ACHARQ_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411204.1"
                     /translation="ISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQY
                     QLGLVDNFNNIS"
     gene            189..623
                     /locus_tag="ACHARQ_002830"
     CDS             189..623
                     /locus_tag="ACHARQ_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411205.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1240..>3566
                     /gene="esp"
                     /locus_tag="ACHARQ_002831"
     CDS             1240..>3566
                     /gene="esp"
                     /locus_tag="ACHARQ_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8411206.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1299 a          509 c          686 g         1072 t
ORIGIN      
        1 aatttctacg gctaaaataa tgttgaaatc aaaccatact atcagtgaag tttctttagg
       61 tttaggattt tcagattctt cacacttttc gagagtattc aaaaagtacg caggtgtatc
      121 tcctaagcaa tatcaattag gtttagtgga caatttcaac aacattagct gagaggtttt
      181 attttattat gagactatat catttacgta gtttggagga gttgaggaaa ttggatttta
      241 acaaaaaatt tttttgggaa atttctgatt tgagttgttt cgtagaatct gagcgtctaa
      301 gtaatggtaa taatagtgtg ttaaatgaat atgacaaagt agtaattaat aaagtaaaaa
      361 gtttatgtga agaagctaga gaatctatca gtcatattaa tggtagcaat tttaaacaaa
      421 cattatttaa tctgattaag ctagatgcta aaatatcaag ttatttattc tttttgatac
      481 atgatgaatt tatggattat caaaaattgg atcagttaat tgaaagagaa agctgggaag
      541 attattacgt gggtctaact ttttcagaaa agttaaacaa ctacaagtta atagattata
      601 agtatttgag tgaaagcaat taattattca tgtagagtac tagacacttt aaatatggac
      661 aattcaatta taaagaaaaa gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt
      721 tgtttcgcta aaaacgagtg aaaaaatgtt gataactcca atcattcgtt taagattcta
      781 ggaatcattc agaaatgctg tgaaaccgat actttataat tataaagaag gtgatttcaa
      841 attccgaaga aggaatttga agtcaccttt ttacttatgt cacagtctca ttcagtaatg
      901 cgggataagg ctccttattc cgttatttga atactctgaa gtttaatcta agaaagggta
      961 atattttgaa aacatgcttc atcgcataga aatgaactta atttcagaaa gaatacatac
     1021 atatcttttt atatgttttc agctgctgaa tggttaaaaa gcacaaatat ccgaaaaaaa
     1081 catgaagaaa tgtacttttt ttgttgtaat gttcatgaaa ttctctaagt aaattagtta
     1141 acataaggtt agtaagattc ctattctgtg aataatacgt atcagattta tcttttattc
     1201 ttactaggaa agtaattatt aagggagttg atttgaataa tggttagcaa gaataataag
     1261 agagtatttc tagaaaaaac aaagaaacga gtattgaagt acagtatcaa aaaattaagt
     1321 gttggggttg catctgtttt gattggtgtt ggtcttgtct tgggaacaac tgaactagtt
     1381 aaagcacaag atgaaatttc tccaagcagc ccacttgaaa cagcgataag ctccgtacaa
     1441 gtaggtgaca aagtagcttc ggggaatact tttcaggaga atcctggtta tacgaaaaat
     1501 tataactttt cggatttaca atttagtcct caagaactaa ctggagacac actaaaaggc
     1561 aatacaattg gatttgaagt ttacggaaaa cataatattg cagcatcaac taaaaactgg
     1621 gaaatccgtc ttcaattaga tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat
     1681 ccaaagaaag gaattggatc ttctagacgg acctttgtaa gaattaatga ttcgctgggt
     1741 agacctacaa atatttggaa ggtcaattat attcgtgcaa gtgatggatt atttgctggg
     1801 gcagaaacaa ccgatacgca aaccgctcct aatggggtaa ttacgtttga aaagagccta
     1861 gatgaaattt ttaaagagat aggcatagac aatctcaaga ccgatcgttt aatgtatcga
     1921 atctacttgg taagtcatca agacgatgat aaaattgtac cgggtattga tagtacgggc
     1981 tactttttaa cagattcaga tgatttctat aatagcttag atgtatctga aaataatcct
     2041 gaccagttta aacatggatc agtaagtgca aaatatgaga aacctaatac tcaaacaaaa
     2101 gatggttcag gatccacagg tgctaatgga gctataatct tagatcataa gttaacgaaa
     2161 aattataatt tctcatattc atcttccgca aagggcacgc cttggtatgc taattacaaa
     2221 attgatgaac gattagttcc atatgtagcg ggtatacaga tgcacatggt tcaggctgac
     2281 aaagtaacat atgatgtttc gtttgaatcc ggaacaaaag tagctgattt agcaatcgag
     2341 cgacgggaag gtcatgaaaa ttatggcata ggttcaataa ctaataatga tttaactaaa
     2401 cttatcgatt ttgctaatgc aagtccacgt ccagtcgtta ttcgatatgt tttacaattg
     2461 actaagccat tagatgaaat cttagaagat atgaaagcaa cagcacaagt tgaagaaaat
     2521 aaaccatttg gtgaagattt catctttgat tcttggttgt cggatacgaa taaaaaatta
     2581 atccagaaca cttatggaac aggttattat tatttgcaag atattgatgg tgatggaaac
     2641 cctgacgata aagaagagag cggagacacg aatccatata tcgggaaacc tgaattagaa
     2701 gaagtatatg atgttgacac aacagttaag gggaaagtat tcatccacga gttagcggga
     2761 acaggtcaca aagcccaact tgttgataaa gaaggtactg tattagcaga aaaaactatc
     2821 gctccaaatg aaaaagatgg ggctccaatt tcagatactg tagaatttga atttacgggt
     2881 gtagattcaa gtaaattaat cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt
     2941 tttgataaac cagaagaagg ttcaaccgtt attaaggaat caccaaaagc ggttgataaa
     3001 caaaccgtgg tagttggatt taaaccagat gctaaagaat caattcggaa taataaaaac
     3061 ttacctgaag atgcagagta ttcatggaaa acagagcctg atacttctaa cgttactgat
     3121 agtacgaaag gtattgtaac tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag
     3181 tttgctgtaa aagcttctca agctatggaa aatgatgcaa catacgtacc tataacaaca
     3241 actccagaaa cgacagttca aagtggaaaa cctacatttg ataaaccaga tgttccttta
     3301 gctaaagatg ccttttcaat ttcagatgtc tataataagt acttcggtaa tgcaagtgtt
     3361 gatgcaaaca ctggtattgt tacattcact ccagctaaag gtgtaggaga atcggagccg
     3421 attactggaa caattcctat taaaattgtt taccaagatg gttctgtagg cacgaccgat
     3481 ttagcagtaa ctgtaagtaa agatatttat gaaaatccag gagaaaacat tcctgcaggc
     3541 taccacaaag taaccttcac cgcagg
//
LOCUS       DAYYAG010000193         3560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000193 DAYYAG010000000
VERSION     DAYYAG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.198"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..254)
                     /locus_tag="ACHARQ_002832"
     CDS             complement(<1..254)
                     /locus_tag="ACHARQ_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8411207.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="ACHARQ_002833"
     CDS             complement(244..1443)
                     /locus_tag="ACHARQ_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8411208.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..2949)
                     /locus_tag="ACHARQ_002834"
     CDS             complement(1573..2949)
                     /locus_tag="ACHARQ_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8411209.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2949..>3560)
                     /locus_tag="ACHARQ_002835"
     CDS             complement(2949..>3560)
                     /locus_tag="ACHARQ_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8411210.1"
                     /translation="SKEKVLSGVNQKFELGKFYAIVGKSGTGKSTLLSLLAGLDKPQT
                     GKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIRLVNKSADESILFELG
                     LDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTL
                     AKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT         1093 a          671 c          503 g         1293 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      421 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2821 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
     2881 aataactaat atgataatta atgattttaa actttttcta gttacataag caaatgcatt
     2941 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
     3001 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
     3061 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
     3121 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
     3181 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
     3241 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
     3301 ctaatatttt caattggtga taaataatct attaaattat agttttgaaa tactaaagat
     3361 atattatttt ttctatgatt actatatcct ttattttgta tatcttcatt cttaaacaat
     3421 atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct
     3481 gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct
     3541 gacaaaactt tttctttaga
//
LOCUS       DAYYAG010000194         3493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000194 DAYYAG010000000
VERSION     DAYYAG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.199"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1337
                     /locus_tag="ACHARQ_002836"
     CDS             6..1337
                     /locus_tag="ACHARQ_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8411211.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1303..1653
                     /locus_tag="ACHARQ_002837"
     CDS             1303..1653
                     /locus_tag="ACHARQ_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411212.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1745..2164)
                     /locus_tag="ACHARQ_002838"
     CDS             complement(1745..2164)
                     /locus_tag="ACHARQ_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8411213.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2165..2509)
                     /locus_tag="ACHARQ_002839"
     CDS             complement(2165..2509)
                     /locus_tag="ACHARQ_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8411214.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2859..3455
                     /locus_tag="ACHARQ_002840"
     CDS             2859..3455
                     /locus_tag="ACHARQ_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8411215.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          909 a          640 c          636 g         1308 t
ORIGIN      
        1 gtgtaatgga aaacgaaact gacgttattt atattcatcc tcaaaaacga atcgtttcac
       61 agaaaagaaa gtatttttat cttggtttta caggtgtttt ttttcttttt attggcttgc
      121 tttctaatac tcctactgat aattggagtg gattgctaac tattcttacc tcgccaagca
      181 atcttttgac agattatttt gctttaggtg ttttcggaag cgcattcata aatgtaggga
      241 ttttgacctt actttctgtg cttttagctt atcggcacaa ggtgatttta aacggtccct
      301 tatttgcttc gattctaaca gttaccggtt tctctttttt tggcaaaaac ctttataatt
      361 ctatctcaat cattttaggt gtgtatctat acgctgtatt tgtcaataaa cctttttctc
      421 agtacatcat gatcggactg tttggtagcg tattatcacc ggtcgttagt tatatcactt
      481 ttggtatgcg ttttccttta ctgataggta ttttattggg gaatttagct ggcatagcaa
      541 ttggtatact tcttccgccc ttagctgctc aaactttagt tttccatcgt ggattcacac
      601 tttataatat tggattcacc tctgggttga ttgcgatgac ttttactgct gttttgaggt
      661 tattcagcta ttcaatcgtc gaaaatacat tggttttcaa tgaatatcac tttcctctca
      721 tttggattat ttttggcttt ttttcgctga cagtcggaat tggtttctat tataattcct
      781 ttcggctttc ggggattcga gaaattttcg attcatctgg aaaattgaca actgatttca
      841 ttgctaattc tggtattggt gctactttga tcaacatggg cttagttggt ctgatgctgt
      901 cttcttatgt tttgctggtt ggcggtcagt taaatggacc tgtcatcggt gctattttat
      961 ctgcggtggg tttcagtgct tttggttgtc atttaaaaaa cagttttccg atactagtcg
     1021 gaatatttat tgcttctttg tttggaactt ttcatgagat caccagtact ggcatgcttg
     1081 ttgcagctgt gtttgggaca ggtcttgcgc ccataagcgg tttttacggc agtttttacg
     1141 gtgtaatagc tggtgtgcta catattgctc ttgtccataa cgtcagcact ttgcacggag
     1201 gactaaatct atataacagt ggtttttcaa ctggtttcgt tgcaggtatc cttgtaccga
     1261 tactagataa ctttaccgct gtcagaaagg aaaaaaagac acttggaaaa agaattatca
     1321 aaaagaatca tcgatgagct tcttacttat ttatattccc atgaaataac tgaaatacgt
     1381 attggagtag aatttgctga ggaagggctt ttcatagaga tacagggtac ggcagaaact
     1441 cagccagaag atttagatgt acttgctgat ttgctgaaca atcctcggga ccctagtatg
     1501 gaaatctact atcaagaact attaggtaat ccccgacatg atcgggaaga ttatcatctg
     1561 ttaggtagtt tgattgatga cgtggaaatc atgtatgccc agccgattat caatattaag
     1621 atctttcgac gtgcttctca ttcctattat taaccctatt ttctatttat aaaagactga
     1681 atcaaaaaag cagctcctga aagttcgatt aaaatctact ttcaggagct gttcaagctt
     1741 gacattattg attttccaat ccttcataca cttcatctgt tacaggttct aaaaactctg
     1801 aattaccagc agtgatcgcc aaatgttcaa accaactgtc ttttgtcgca ccatgccaat
     1861 gtttaatccc tttgtgcgtt acgacgacat ctccagccat taatcgtctt gctggctttc
     1921 cttcttcctg ataccagcct tctcctccgg tcacaagtaa gatttgatag ccgtctgtat
     1981 ggacatgcca gtgattgcga caacctggtt caaaagttac attacctaca ccaacagaaa
     2041 cttcgggatc attcactaac atattcagat agctttgccc ggtaaatact tgagcgtaag
     2101 cttcgttttt ttcacctgct ggaaaaatca cgccattgtg tatttcttcg tgttttgtca
     2161 tcatttacgc ctctttctta tcttcgctat tttcttctaa atagatttct tttgctaaac
     2221 taaaagctga ccaagctttt ggccattcta cataaaaagc taaatgggta atgatttctg
     2281 ctatttcttc actagtaatg ccattttgct tgccaatctt cagatgagct ggcaattgtt
     2341 caaaatttcc tcctgaaatt aatgcagata tggtgatgat actacgctga tgagcagata
     2401 attcttctct ggaccatact tgtccaaata aaacatcatc atttaattct gcaaattttg
     2461 gtgcaaaatc tcctaattga tctcgtcctg cggtttgttt ttctgccatt tctcttattc
     2521 tcccttcacg actgttaggt gattcaacta tggttctaag gcaactttat tttctattgt
     2581 ttcttcgcat ttcattttaa atgtagtctt tcagcctgtc caatgcttag tttattcagt
     2641 cgatatgctt tttaagcata gttgctaacg ctttttttat tttaaaaaaa acaagttatt
     2701 aaataagtct ttagttggat gtttttttga gatcaatctt ttatacttat gatgaactta
     2761 caaaattgtt tgttttctgt catatcaaaa aatggcgcag ttagttgctt ttctagtaaa
     2821 gtatagttaa caaagaacct ctaaggagtt gatgaataat gaatattgct gcctctcgaa
     2881 aactgataaa tttcatatcc attatcggat taggcctatc aatcgcgctg acgatttatt
     2941 ttattaattt aggtgttttc aaagatttga atgcgttaag aggattagta ggtgattcca
     3001 tcattttagg accgattatt tttgtcttca tccaaatcct gcaagtagtt attccaatca
     3061 ttcctggagg gatcagtact gcagctggtg tcctgatatt tggcccttat gctggattta
     3121 tctataacta tattggaatc tgcattggtt cgattatcat ttttctgcta gggcgtcgtt
     3181 atggaaagcc attcatactt tccatgatta gcgataagac atataacaaa tatattggct
     3241 ggctggataa tcaaaaccgt tttgagaagc tgtttgctct agctattttt cttccagttg
     3301 ctcctgacga tgcgttatgt ttgatggctg gattgacaaa catgtcagtg aaacgatata
     3361 cactaatcat tttgattgct aaaccactgt cgattttctt atatagtatg gccttgattt
     3421 atggaggtca atatctaagt ggtcttcttg gataatattt attttgtaaa attagtcatt
     3481 gattgacaat gtt
//
LOCUS       DAYYAG010000195         3487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000195 DAYYAG010000000
VERSION     DAYYAG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.200"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..754)
                     /locus_tag="ACHARQ_002841"
     CDS             complement(251..754)
                     /locus_tag="ACHARQ_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8411216.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1113..2138
                     /locus_tag="ACHARQ_002842"
     CDS             1113..2138
                     /locus_tag="ACHARQ_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8411217.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2461..3339
                     /locus_tag="ACHARQ_002843"
                     /pseudo
     CDS             2461..3339
                     /locus_tag="ACHARQ_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
BASE COUNT         1127 a          556 c          660 g         1144 t
ORIGIN      
        1 ggaattcctt tctagattga gatattgact aggaagaatg ttaagatttt aagcctttaa
       61 gctaaaaaaa aacccttttt gttaaataag gggaatatag cctgtcagtt tcaaactgca
      121 agtcgtcctg ctgcaataat atagtagatt agagatgaaa atagattttt ttaaaccaat
      181 gactgaataa aaagaccgtc atagatacac attggaatgt gtctattacg gtcttttttt
      241 acgaacgatt ttattgaaga cggacagttc gggtcaaaaa taaaaaagct acgatcagaa
      301 ataagaataa aatgtatccc aaaataatgg ctagacggtc taacttattt gtcgatagat
      361 gtttgtttag aagttgatag gcccataaac ctatcagtcc tcctgttgta ttagttatca
      421 aatcagtgat gtccgctacg ccaataccga ataggaattg aatcaattct gccaagaagc
      481 ttacaaggaa aataatccct aattttttag aaaaagaaag tctcttgaaa ttgacattca
      541 aaagcacccc aaaaggaaaa aagacaatca agttataagt ggtctcacgt aataccgtcg
      601 tattttcaaa agaaaaagta gaaaaaggga cgagattgac attgctgtaa gaataagtga
      661 gaaattgtgg aagattaaat gatagcttga ataaaatcaa ccataataga aagaataaat
      721 aaatccctaa taaaaatttt gatacatacc gcatagacat ctcctccatt taaaaagtat
      781 accatcgaaa accgctgaaa aatcataaaa aagtatgaag tttaaggacc aacgtagtta
      841 tcgtacgaaa attagaagct tttttttgtt gggaatccgg tttctttaac aaagctaaga
      901 tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg gaatgttcgt
      961 tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg aaaaatcaag
     1021 aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc ttggttcagc
     1081 tttttttcca attactagga aggagggata gattgttcaa gacttatttt cgtttcaata
     1141 aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt attgtattat
     1201 ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta gggatcttta
     1261 cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg tttctgatcg
     1321 gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt ttatggcttg
     1381 aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga ttattccata
     1441 tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg agcatgcagc
     1501 tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc gtttttgccc
     1561 tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca attgaaaaag
     1621 caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct gttttctaca
     1681 gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat aatccttatt
     1741 ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg atgaagcatc
     1801 gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat
     1861 ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat ggaatcgcac
     1921 aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg atggcgttga
     1981 tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat cgttttttag
     2041 ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc
     2101 aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa
     2161 agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa agccagattc
     2221 tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa aattttatat
     2281 ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct gagatggtca
     2341 atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa gttttatagt
     2401 tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt catttaaaca
     2461 atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa
     2521 attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat tgtacaagca
     2581 aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga tcctaataaa
     2641 gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt tgcagcatta
     2701 gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt
     2761 ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca tgaacatttc
     2821 caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta cactagtggc
     2881 ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa agggtacacg
     2941 gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga tgcagattta
     3001 tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga actttatttt
     3061 cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc caatcttact
     3121 aaatagttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg attattatca
     3181 gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga tgcccaaata
     3241 catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt gcttgcaggc
     3301 gagcacattg gctctggctt aggtattctt acttcataag tttagtactt cgaaatagtt
     3361 taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     3421 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     3481 aattttc
//
LOCUS       DAYYAG010000196         3380 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000196 DAYYAG010000000
VERSION     DAYYAG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.201"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHARQ_002844"
     CDS             complement(<1..2232)
                     /locus_tag="ACHARQ_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8411218.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHARQ_002845"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHARQ_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          933 a          752 c          542 g         1153 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt
//
LOCUS       DAYYAG010000197         3335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000197 DAYYAG010000000
VERSION     DAYYAG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.202"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHARQ_002846"
     CDS             complement(451..1761)
                     /locus_tag="ACHARQ_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8411219.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACHARQ_002847"
     CDS             complement(1773..3191)
                     /locus_tag="ACHARQ_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8411220.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1032 a          676 c          515 g         1112 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataag
//
LOCUS       DAYYAG010000198         3162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000198 DAYYAG010000000
VERSION     DAYYAG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.203"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHARQ_002848"
     CDS             complement(63..2555)
                     /locus_tag="ACHARQ_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8411221.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHARQ_002849"
     CDS             complement(2562..3029)
                     /locus_tag="ACHARQ_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8411222.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAYYAG010000199         3080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000199 DAYYAG010000000
VERSION     DAYYAG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.204"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..193
                     /locus_tag="ACHARQ_002850"
     CDS             <1..193
                     /locus_tag="ACHARQ_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFM8411223.1"
                     /translation="AAVKRLAMKNSEETFVLIDHTKFNQLSFTSVAPLEAATIITERC
                     PLEFVNEFREKTTIKEAIQ"
     gene            190..1104
                     /gene="pfkB"
                     /locus_tag="ACHARQ_002851"
     CDS             190..1104
                     /gene="pfkB"
                     /locus_tag="ACHARQ_002851"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8411224.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1122..3053
                     /locus_tag="ACHARQ_002852"
     CDS             1122..3053
                     /locus_tag="ACHARQ_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8411225.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
BASE COUNT          965 a          563 c          719 g          833 t
ORIGIN      
        1 ggcagctgtt aaacgtttag ccatgaaaaa ttccgaagag acatttgtat tgattgatca
       61 taccaaattc aaccagttgt ccttcacctc tgttgcacct ttggaagcag ccacgatcat
      121 aaccgagcgt tgtccattag aattcgttaa tgaattccgt gaaaagacaa caattaagga
      181 ggcaatacaa tgatttacac agtaacactt aatccatcaa ttgattatat cgtacgcgta
      241 gatgggttaa aactaggcga tttgaaccga atgaaggaag acttcaagtt acctggagga
      301 aaagggatca atgtttcccg tattttgaaa cggatcgatt ctcaatctac agcactaggg
      361 tttttaggag ggttcactgg cgaatttatt tcgaagtggc ttgaaaaaga acaaatccag
      421 tctttgttca caaccgtaaa agaagataca agaatcaata tcaaactaaa atcagatact
      481 gaaactgaaa ttaacggttt gggcccatct atttcagatg aagaaatcga tgagttgaag
      541 caagcattag gaacagtagg acaaggagat atcgtcgtat tgtcaggaag cgcgccggct
      601 agcctgcgaa aaggatttta tcaagaactg attgatatca ttcgagaaaa aggtgcagat
      661 ttcgtgattg atacaacagg tgatgaccta aaaaatgcac ttgttaagaa accattgttg
      721 atcaaaccaa acaatcacga attagctgag ctttacaata caaccttcca atctgtagaa
      781 gaaatcattc cgtatggtaa aaaattgcta gctgaagggg cacagaatgt gctgatctca
      841 atggctggag acggtgcttt gctcttcacg gaacaaggaa cttataaaac gaatgtatta
      901 gttcgcccat tgaaaaattc agtaggtgca ggggattcaa tgattgccgg ctttattggg
      961 gcttacagcc gtacgaaaga tccaatcgaa gcctttaaat ggggagttgc ttgcggaagc
     1021 gcaacagctt tttctgatga cttggcagct cgcgaattga ttgatgagtt gctgccagaa
     1081 gttgaaatca caaaaattga ttagtttcaa ggaggataat catgaatatc aaggatttaa
     1141 tggtcaaaga agcgatgatc atggatttgc aagcgactga taaaaaaggc gcaatcgatg
     1201 aaatggtcca aaaaatgttt gatgccggcc ggatttccga tatcgaaaca tataaagaag
     1261 gaattctagc ccgcgaagca caaacttcta caggtcttgg tgatggaatc gccatgcctc
     1321 acgcgaaaaa cagtgcagtt aaagaagcaa cagttttatt cgcaaaaagc caaaaaggag
     1381 tagattatga agccttagac ggacagccaa cttatctgtt tttcatgatc gcagctccag
     1441 aaggcgcaaa cgacacacat ttgcaagcat tagcagcact atctcgattg ctgattgatg
     1501 cagatttcgt aggaaaatta aaagaagcaa atacaccaga agaagtccaa gacttgttcc
     1561 aaacagctga agagcaaaaa gaagcagaag aaaaagcaga acaagaaaca caagctgcag
     1621 aagcttctgc tcatccagaa agaaaattcg ttgttgccgt tacagcttgt ccaacaggga
     1681 tcgctcacac ttacatggca gaagatgctc tgaagaaaaa agcgaaagaa atgggtgtgg
     1741 agatcaaagt cgaaacgaac ggttcagaag gaatcaaaaa ccgattgact gctgaagaca
     1801 tcgcccgtgc agaaggtgta atcgttgcag cagacaaaaa agtagagatg aatcgctttg
     1861 acggaaaaga actgatcaat cgtcctgtca gtgatggtat ccgtaaacca gaagagctga
     1921 tcaatctagc ccttagtggt actgcaccag tattccatgg tgatggaaaa gaaagcagtt
     1981 cagaagaagg aaatgcggat ggtactatcg gacaaagaat ttacaaagat ttgatgaacg
     2041 gcgtatcaca catgttacct ttcgttattg gtggaggaat cgctattgcg ctttctttca
     2101 tgatcgacca atttattggc gtgccacatg atcaattatc aaatctaggg aattacaatc
     2161 aagctgctag ctggttcaac caaatcggac aagctgcatt tggtttcatg ctgccggtat
     2221 tagcaggatt tattgcttca agtatcggtg accgccctgg tttgatcgtt ggatttgctg
     2281 ctggtgcatt agctaataca ggcggtgcag gattcttagg tgcactgatt ggtggattct
     2341 tagctggtta tgtcatcgta tttttacgta aattattcaa gaacttacca aaatcattgg
     2401 atggaattaa aacgatcctc ttttatccag tgtttggctt actgattact ggtttcttga
     2461 tgttgctggt caatgtccct atgaaagcaa tcaatgatgc attgaacggt ttcttgacaa
     2521 gtatgagtgg ttcgaatgct gctttacttg gtgcattatt agctggaatg atggcggcag
     2581 atttaggcgg tccaatcaac aaagccgctt atgtgtttgg tacagcaact ttagctacga
     2641 ctgttgcaac tggcggaagt gtcgtgatgg cttctgttat ggctggagga atggtacctc
     2701 cattggcaat tttcgttgct acacgtttgt ttaagaataa attcagcaaa acagatcaag
     2761 atgctggttt gacgaacatc gtgatgggac tatcgtttgt cacggaagga tcgattccat
     2821 ttgcagcagc tgacccgatt cgtgccattc caagttttat tattggttct gctttaactg
     2881 gcggattagt cggagctttc gggatcaaat tactggctcc tcatggcggg attttcgttg
     2941 tattcttact gagtcacccg gtcatgtatt tggtgtttat tgctatcgga gcagtcgtat
     3001 ctggtgtgat ttatggttta ttgagaaaag ctcctgcagc agcagaagca tagaaataaa
     3061 aaagaagagg ctggtttttc
//
LOCUS       DAYYAG010000200         3065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000200 DAYYAG010000000
VERSION     DAYYAG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.205"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            239..2299
                     /locus_tag="ACHARQ_002853"
     CDS             239..2299
                     /locus_tag="ACHARQ_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8411226.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2382..2951
                     /locus_tag="ACHARQ_002854"
     CDS             2382..2951
                     /locus_tag="ACHARQ_002854"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8411227.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1113 a          543 c          574 g          835 t
ORIGIN      
        1 ctgatttatg tttttttcaa agaaaaggta actagatata actagatata actagatata
       61 ttacttaaca aaattaaaaa gtacataagt tattattccg attttcttga aacaataaaa
      121 aatcaaaaaa aatactctcc taatagtcca tcttcttttg attttattta tataatgaaa
      181 tcatcttatg aaaattacta cttcatgttt agacaagagt agggaggact aagaataaat
      241 gtgttcaaaa agacgttcaa aacaactaaa taaacacgct tgtcgtttag ataaggaacg
      301 gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc acgatgctga tcagtttatc
      361 tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca caaaccatca aggtcgtacg
      421 tacctctgtt tctgagacca ctgattcccc tatcactatt actcatactt tcactgatgc
      481 aaatggaaaa cagctacttc ctgcattgac ttcctataat aaagtcagtt atcctccaac
      541 gataaaccac tactggttaa ttcctaacaa aacattatgg acatcggaaa ccggaagcag
      601 tgtgccgctt tctatctttt ccgatatgac tgatcctaca aaaggtgatc agttaaaaca
      661 gatcactgat attttgaatc atgctttgac agatataaag gacggtggaa gcgcacaagt
      721 ttcttatgta tatgaaaaag attattcaca gtttgaaaga aagaaaatcg atgttctggc
      781 agggacaaaa gtttcgatat ctgatctggt agaaaggctg aagaacgcag atggaacaca
      841 aggagatatg tccaaagtaa tttcaaacaa tgggaatatc gtggatacga ctaatacagg
      901 aacatctact atttccttgt cctattttga tccaattgct ttaaaaacaa tgactgcatc
      961 tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc ttcacttaag
     1021 aacaggagag agttacgccc ctaaagatct tgttgaatat gtaacaaaaa gcgatggaac
     1081 ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta gatactacaa ctccgggaga
     1141 atatcccgtt gtattaactt atatagactc gaaaacatca aatgtggcag aaacaacagc
     1201 tgtagtttat gtagaagcat ctgctgcttc atcgactgtc atcactcatt ttatagatag
     1261 tcaaacaaat aaagaacttc aagcagaaac acatatgagt agtgtaaatc gtattcctga
     1321 tttgcgccta cctgctgaat tttctgatcg agtgccaaat atagatttat cgacttatga
     1381 acaaaatggg aaagtcactt ctatttctga ttggatgcaa gcaaacggat tagacagaaa
     1441 aagtcactgg tcggagatcc ttgaagtact tcactcagaa ctgatgaata ataggaagca
     1501 atcaggaggg gaaattacct ttacttatgt ttatatacca gacaatcgtt cattagaagg
     1561 tcatgatctt attgttcatc caaacgaaaa aataaacaaa gaagagttga tccaatcagc
     1621 aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa tcaatgaagt
     1681 accattggag gatggttatt tgaccaaaca aacaggtgat tttacgatta tgtatagtta
     1741 tcatgacccg tattcactcc aagaactcca tgctcagtca ctattacatg ttttaccatt
     1801 aaacaaacag atagatcaaa aagaacaaaa aagacaagaa gaaagcttgt cagaaaaaaa
     1861 acaacaaatg gctcatgcag tttctccaac agggaaagta aaacaaacaa gaagcctaga
     1921 gttagcaaga atatctagaa aaatctcaga atctaataaa acgataaggc aagaagctca
     1981 tttggcccca caagtttctg gattggaaaa tgaaatttta gagcctaaaa taaaacaaca
     2041 atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag aaggaatcag atgaccagaa
     2101 ggaaacgaag aaaaaacgct tgtcaactat tgattccacc gatgagcatc tgcagccgca
     2161 agtaccactt cctagtccgg gaacacccgc acctggtgga ggtattcctt cttctgcttt
     2221 aggagatttc atgcgaggta tggcaggaac atgggtatac gctaatcgaa atgaatttga
     2281 cgcagatgac tggttctgat ttatatctct atctgttata gtcaaataag tagctgagtc
     2341 atatgttatt tggcataatg gagacgagga gggatagaaa aatgttagat aaaacgttac
     2401 cttacgcaga aatctggatg acaagaccac ttcaacaaaa tgttccaatc acgcctttag
     2461 cggaaggttt tgaatttcga ttttatcaaa aagaagatga atctgcttgg gcggagattg
     2521 agacttccgt tggggaattt caaacgacag atgaagcact tgagtatttt caacaaaact
     2581 ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca aaaaaaggag
     2641 aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg tatccggttt
     2701 ttcattggtt agcagtgaaa tcagattacc aaggaaaagg gctcgcaaaa gcattgactg
     2761 ccagagtctt gcaagaattt cctaccctcc atacggacgg accaatctat cttcacacac
     2821 agacatggag ccatcaggcg attgctcttt ataaaaaaat gggttttact tttattgctg
     2881 aaaatctaga cggcacacct aatccagatt atgaaaaagt tatgcaggtt ttaagtaaat
     2941 tagataaata acgaaatggt gaaataagtc ctcagtattg ggataaaaaa cgaaatagtc
     3001 attcctcata tcttactttt ttgagttaat actatcggat aactaatggc atgccataca
     3061 ttcaa
//
LOCUS       DAYYAG010000201         3024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000201 DAYYAG010000000
VERSION     DAYYAG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.206"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1758
                     /gene="alsS"
                     /locus_tag="ACHARQ_002855"
     CDS             94..1758
                     /gene="alsS"
                     /locus_tag="ACHARQ_002855"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8411228.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1771..2481
                     /gene="budA"
                     /locus_tag="ACHARQ_002856"
     CDS             1771..2481
                     /gene="budA"
                     /locus_tag="ACHARQ_002856"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8411229.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2611..>3024
                     /locus_tag="ACHARQ_002857"
     CDS             2611..>3024
                     /locus_tag="ACHARQ_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8411230.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDE"
BASE COUNT         1007 a          506 c          687 g          824 t
ORIGIN      
        1 cttttgaatg aaacaggttt agaaactgta tcacttattt gtgattattt ctaactaata
       61 attaaaaata aagcataaaa aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg
      121 aaatctgtag tagaaagttt gattaatcat catgttgatt atgtattcgg tataccaggt
      181 gcaaaaatcg atggtgtatt taatgaatta gaggatcaag gtcctgaatt aatcgtgact
      241 cgtcacgaac aaaatgctgc atttatggca caagcaatcg gacgtattac aggagagcca
      301 ggagtagtta tcgcaacaag tggtcctggt gctagtaact tagctacagg tttggtaaca
      361 gcgacagcag aaggtgaccc agttttagca attgccggac aagtaaaacg aagcgatcta
      421 ttgaagttga cccatcaaag catggataat gcagcactgt tccagccaat cacaaaatac
      481 agtgcagaaa tccaagatcc agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg
      541 gctaagagtt caaaaaaagg cgcgagcttt atcagtatcc cgcaagatgt agtggatgca
      601 cctgtccaag gtaatgtcat caagccattg agcgatccta aattaggttc tgcttctgca
      661 gatgatatcc gttacctagc tgaacgcatt agagaagcaa aactaccggt actattagta
      721 ggtatgcgag gatcaagtga aaaagaaacg ttagccatca ggcaattagt tgaaaagaca
      781 gcattgcctg ttgtagagac attccaagca gctggagtga tctcacgtga attagaagcg
      841 catttctttg gtcgtgtggg tttatttaga aaccaaccag gagatatgct tttgaaacga
      901 agtgatctag ttattgcaat tggctatgat ccaatcgaat atgaagcaag aaactggaat
      961 gcggaaaaag atgcgcggat cattgtgatc gatgaagcgc cagctgaaat cgatcctttt
     1021 atgcagcctg aaagagaatt gatcggtgat atctcagcca ctcttgattt attgacaggt
     1081 tcattagagc ctcaacaagt gtcagaagat gcaaaagaat atttagcatc tcttcaagca
     1141 aaattaacgg agcgggacat tgttcaatca aaaggagaag caggtatctt gcatccttta
     1201 gaagttatta acacgcttca gtcaaaagta acagatgata tgactgttac cgtagatgta
     1261 ggtagtcatt atatttggat ggcacgccac tttagaagtt atgaacctcg tcatttgtta
     1321 ttcagtaatg gtatgcaaac gcttggtgtt gcattgccat gggcgatttc tgctgcattg
     1381 gtgcgtccaa atacacagat cgtatcagtt tccggcgatg ggggattttt attctctgcc
     1441 caggatttag aaacggcggt acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat
     1501 ggacactaca atatggtaga gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta
     1561 gactttggtc cagttgattt tgtgaaatat gcagaagctt ttggcgctaa aggaatccga
     1621 gctacaagtg tagaggaatt agaaaaagct ttagaagaag gatttgcgac agaaggaccc
     1681 gtaattattg atattccgat tgattaccgt gataatgaga aattgggaga aacaatctta
     1741 ccagatcaat tctattaagg aagtgtcgac atgactgaaa aaatcttata ccagcacggg
     1801 acattaggag ctttgatggc cggcttgatg gatggaacag aaacgattgc tcatatatta
     1861 gaaaaaggaa ctctgggatt gggaacattg catgggcttg atggtgaagt catcttttta
     1921 gatggcgtag cttatcaagg aagatcagat ggctcagttg cccaattaga tggaagtgaa
     1981 cttaccccct atgcagcaat tacagatttt acgccagata ctagtttttc tgtctcaaaa
     2041 actgcagatg gagaacaatt gaaaaaagac atattagtaa gagaagccgg ggaaaaccta
     2101 tttttggcag tgaagataac tggattattc aaaaatatgc acatccgtat catgccgaag
     2161 caagaaaaac cttacagaag actagcaaaa atttcagagt ctcaaccaga attccaacaa
     2221 agcaatgtcc aaggaacatt agtcgggttc tttacgcctg aattattcca aggagttgct
     2281 gcagcgggat ttcacctgca ttttatcgat gatacacata catttggcgg acatgtaatg
     2341 gattttgaag tggctgaagg aaaagttgag attagccgca tctcgtccct tgtacagcat
     2401 tttccagttg atgatccgac ttttgttcac gaaacgattg attatgctga tttagcagca
     2461 gaaatccaac aagcagaata gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac
     2521 gtatctcgtt tttttgagga gaaaacctag cgtttacaaa aaaactgagc tataatcttc
     2581 tccatagatc aaaaaggagc ggtttttatc atgtggacag aaaaacagaa aaaagatgca
     2641 tacgaaattt tattattaca gcaacaagga ttattagaag cagaagataa ctggttagca
     2701 aatttagcaa attcatctgc attgttgaat gagactttgc ctgagactgt ctttgcagga
     2761 tattatttat tcgatggtaa tgaattgatt ttaggaccgt tccaagggcg agtttcttgc
     2821 acacgtatca agatgggaaa aggagtttgt ggagaatcag ctgaaaaaca agaaacattg
     2881 atcgttgatg atgtcaaaac ccatgcaaat tacatttcct gcgattcagc cgcacaatca
     2941 gaaatcgtag tgccaatggt aaaagaaggt aatttaatcg gtgttttaga tatcgattgc
     3001 ggagtaacga aagggtatga tgaa
//
LOCUS       DAYYAG010000202         2958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000202 DAYYAG010000000
VERSION     DAYYAG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.207"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..1005
                     /locus_tag="ACHARQ_002858"
     CDS             286..1005
                     /locus_tag="ACHARQ_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8411231.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            1007..1849
                     /locus_tag="ACHARQ_002859"
     CDS             1007..1849
                     /locus_tag="ACHARQ_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8411232.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            1846..2796
                     /locus_tag="ACHARQ_002860"
     CDS             1846..2796
                     /locus_tag="ACHARQ_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8411233.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            2811..>2958
                     /locus_tag="ACHARQ_002861"
     CDS             2811..>2958
                     /locus_tag="ACHARQ_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HFM8411234.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKL"
BASE COUNT         1066 a          366 c          545 g          981 t
ORIGIN      
        1 tataaatgtt ttgatgctta tcaacttatt caacccgcta tccacttcaa gtataatttt
       61 tccatacgcc aataaagtag ttttaggagc atgttctcat ttgagaacgc tcctttttta
      121 tatattttga aagctactta ctatacgcgt agtttcaaat agttttgtca ttctaatctt
      181 cagcaacaga aaaaaatcta aactataaaa tttagggttg actaaaatat aatttttgta
      241 taccatatga tcgtgataat cattatcatt tagaaggaga aagttatgtt agaagtgaaa
      301 aagcttaatg tttgttataa cgatttttta gcattgaacg agatttcttt aaaaatacaa
      361 gaaggtgctt taacaggtat tattggtcca aatggtgctg gaaaatccac attgcttaaa
      421 gcaatgttaa atattatccc tcatcaggga gaggtctcca ttaataatga agatatcaat
      481 aaaaaactgg tgaagattgc atatgtagag caaaaagctg atattgattt tacttttcca
      541 ataaaagtaa aagaatgtgt atctatggga acatatgccg gaatgaaagt ttttcaaaga
      601 attaaaaatg cagaatggca aagagtttca aaggctttgg aaaaggtcga tatgggaaaa
      661 tattctaatc atcaaatagg agaattatct ggaggacaat ttcaacgtgt acttttagca
      721 agatgtcttg ctcaaaatgc ggatttcata tttttagatg aaccatttgt agggatagac
      781 ttagttagtg aacgaataat tatggatacg ttaaaaaaat tgaaacatca gggaaaaaca
      841 gtgctaattg tgcatcatga tttaagcaag gtaaagaagt attttgataa tattattctt
      901 ttgaatcgaa atctaattgc tcacggaagt gtagaatctg tatttaatga agaaaattta
      961 aaaaaagcct atggagatac tatatttatt ggagaggggg aataatatgc ttcaaaattt
     1021 tattaatggt ctgtatgact ttcacttttt acagaatgct ctaattacat ctgtagttat
     1081 agggattgtt tctggaacag taggttgttt tattatttta agaggaatgt ctcttatggg
     1141 tgatgctatt tctcatgctg ttttgccagg agtagcaatt tcttatattt taggtattaa
     1201 tttttttatt ggtgcgattg tatttggtct attagcttct atcattatta cttttataaa
     1261 aagcaatagt gtaataaaag gagatacagc aattggtatt actttcagtt cttttttagc
     1321 actaggagtt atccttatag gagttgcaaa tagctcaacg gatttatttc atattttatt
     1381 tggaaatatt ttagctgtac aagatattga taagtggatt acaattggcg tttcggtttt
     1441 agtgttggtt atagtagttc tattttttaa agaattatta atcacttctt ttgatccatt
     1501 aatggcaaaa gcaataggga tgaatgtcag tttttatcat tatctattga tggttctatt
     1561 aactttagta gcagtaacag ctatgcaaag tgtgggaact atattaattg ttgcaatgtt
     1621 aattacacca gcagctacag catatttata tgcaaatagc ttgaaaacta tgatatttat
     1681 ttctgcaaca gtcggagcaa gttcatcatt gcttggattg tttattggtt atagttttaa
     1741 catagcagca ggatcaagta ttgttttaac agcggcattc ttatttgtaa ttagttttct
     1801 aatctcacca aaacaaaaat ttttaagaaa gaaggagaga tcgttatgat gagaaaatgg
     1861 aaagcagtac tgggaagtct ggggattttg attgctcttt ttatattcgg ggcatgttca
     1921 ataaatagta aagacaaaga taaagtggct tcgaacgaaa aattaaaagt agtagttact
     1981 aattcgattt tagcagatat taccgaaaat atagcaaaag ataaaattga tttacacagt
     2041 atcgtaccta ttgggaaaga tccccacgaa tatgaacctt tgcctgaaga tgttcaaaaa
     2101 acttcaaaag cagatttgat tttttataac ggtgttaact tggaaactgg aggaaatgct
     2161 tggtttacaa aattagttaa aaatgcgaac aaagaggaaa acaaagacta ttttgcagca
     2221 agtgatggca tagatgttat ttacttagag ggtcagagtg agaaagggaa ggaagatcct
     2281 catgcttggt taaatttaga aaacggtatt atttacgcta aaaatattga aaaacagtta
     2341 gcggaaaaag atcctgataa taaaaaattc tataaagaaa atctagataa gtatattgaa
     2401 aaattggatt ctctagacaa agaagctaaa tctaaatttg cttcaattcc gaatgataaa
     2461 aaaatgattg ttacaagtga aggatgcttt aaatatttct cgaaagcgta taatgtgcct
     2521 tctgcttaca tttgggaaat caacactgaa gaagaaggaa caccagatca aataaaacac
     2581 ttagttgaaa aattacgcac aacaaaagtt ccctccttat tcgtagaaag cagtgtggat
     2641 gatagaccaa tgaaaacagt atcaaaagat accaatattc ctatctattc aacgattttt
     2701 actgattcaa ttgcagaaaa aggacaagat ggtgatagtt actatgcgat gatgaaatgg
     2761 aacctggata aaattgctga aggcctttcg aaataatttg gagggtaggt atggtacaaa
     2821 ttaaaacgac tactatacta atttatttac tagcaattca ggaattatca aaaagaaaaa
     2881 agaaattaaa aaataatgat ttagcaaaag ttctaagtgt tagtccagca tctgtaagtg
     2941 agatgctggc taaactta
//
LOCUS       DAYYAG010000203         2895 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000203 DAYYAG010000000
VERSION     DAYYAG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.208"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..1775)
                     /locus_tag="ACHARQ_002862"
     CDS             complement(9..1775)
                     /locus_tag="ACHARQ_002862"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8411235.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1735..>2895)
                     /locus_tag="ACHARQ_002863"
     CDS             complement(1735..>2895)
                     /locus_tag="ACHARQ_002863"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8411236.1"
                     /translation="EYIRGIQVIKIFGAKVTSFKALNKAINDYAKFAYKYSLSGKTPF
                     VLFQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGIIFVSFMRIMYVSMH
                     LFEARYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYKEECVLENFNLVL
                     EQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQ
                     DTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREHTLIGSKGVYLSG
                     GEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMKEKTVIMIAHRLSS
                     IKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRLDNEKFL"
BASE COUNT          937 a          549 c          413 g          996 t
ORIGIN      
        1 ctatttcatc atcgtgattg agcttctttt tcatagacga atttctcaga tagtttagtt
       61 ttctttatga gatttttgta tatttctgac cttcgataca actcattatg agtaccttga
      121 tcaactagtt ttccttgatc catcactaca attttattta ccttttcaat agacttcaag
      181 cgatgcgata taattagtac tgtcttatct tgtattaaac gatttaaact ttcctgaata
      241 cgtttttcat tttccgcatc taaactggca gtaatttcat ctaaaattaa aattggcgca
      301 tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct ctcctccaga aagtgaactt
      361 ccgttttctc cgaccaatgt atgataaccg tcaggtagtt tttcaataaa gtcttcgcag
      421 ttagctaaac gagctgcttt tttcacttcc tcatctgacg cttgactatt tccaatacgt
      481 atattttcca aaatacttgt attaaacaaa gttacctctt gaaaaacaat agcgattttg
      541 ctaaacaaag aagctggaga tacacgttta atatcatacc catcaattaa aatacagcct
      601 tcatcataat catacagtct agacactaat ttcaagatac tagttttccc ggaaccacta
      661 gcacctacta acgcagtcac ttctccttgt ttagccgtaa aagagatatg atctaaaatt
      721 ggggtattgt tatcatatga gaaagagaca tctcgcaact caatgtcaaa agatttcaga
      781 ggactatctg atccctcttg gatactggtt tccttcatag ctctaattcg ctgtatttta
      841 ggagctaaat aaaatatttc aagcactgct tctttcatag aatcgaagga atcctttatt
      901 tttattgcgg ctagtaagta accaacgacg tagagtatag tcacttcacc cgttaacagt
      961 aagtgaacac ctactagaat gactactgct aaactaacat atgagaaaat agaggataag
     1021 gccatgactg aaaaagtact taattctacc tttaaatgaa tcctttcact ctcttccatt
     1081 tttttgaaaa gtctgtcttg aactttttta gatagattaa agctattaat ctcttgctgc
     1141 aattctatag tttcttgaaa ttcttccgag ttctctctca aagtatcgta atatttagta
     1201 ttggctttcg tttgggattt ctttgataac aagattaaga caaaactaaa taacgttggc
     1261 aaaataacag ctaatcccat tttgacatta ccaaccaaaa gcatcactga aataaaaggg
     1321 aaaaacagag ccataccgcc ggctttaggt attgcatgac tcatcgcatg ctcaatacct
     1381 tcaacgtcag acatgatagt ttgagataaa tctgataaat tatgttttga aaaataggat
     1441 aatggtaaat ttgacaaatc gtctgcaatt tgcactctta aatgcgcact ttctttataa
     1501 gtactattat ataagttttc atactcccta tctaacaacc aatacatgaa aagcaaagta
     1561 acaaacgata cggcaaaata aagccaacgg ggctttgtat tttctaaaac taattcgtct
     1621 atcagcatca ttataatgaa tgctggtacc atattaatgc agtatacaaa aaaactagaa
     1681 atagaagctt tagttaaagc ttctgcgccc tgatcactta aagcaaattt gtttttataa
     1741 aaacttttca ttgtcaagcc tccaatcatt tgcattctca taattagtga ccaattgttt
     1801 atacacttga tgtttagcca ttaattcatt atgatttcca cgttctacta ttttcccttc
     1861 tgacaagaca agaatttcat ctacagattt aatgctggat aatcgatgag ctatcataat
     1921 aactgttttt tctttcatca gatgttgaaa agccttttgt agttcatact cattatctgg
     1981 atcaatagat gcactggctt catccataat aacaatcttt gcatctttta aaattgctct
     2041 ggcaatagct atacgttgtt tttctcctcc tgacagataa actcctttac taccaattaa
     2101 ggtatgctct ctttcgggaa atttttctaa aatcgattcg caaccggcta atcttagcgc
     2161 gtcaaatact ttacttgctg gcgcttgagg atttgctata gaaacgttat cgtaaatact
     2221 ctgattgaat agtttcgtat cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt
     2281 tgaatagtga tttaaaggct taccaccaat tttaatctgt cccttatcca cattgtagaa
     2341 acctgaaatt agtttagcta tcgtactctt tccgcttcct gattttccta ctaatgcata
     2401 aattcttttt tgttctaaaa ctaagttgaa attttctaaa acacactctt ctttgtatga
     2461 aaagctgaca ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt tcccatattt
     2521 tagttggtct tgcttcattt ctaaataaag ggagtctagt gtttcgactg catatcttgc
     2581 ttcaaacaag tgcatactta catacattat tctcatgaaa gaaacaaaaa taattccact
     2641 taaaaaaaac aacatcaaca agtcaacagc taatatcttg ggagtggtaa gagaagttat
     2701 aaaaaaggtt ataggaacta tcaaaatgca aactatgcca aaaaatagcc attgaaaaag
     2761 aacaaaaggt gttttcccac tcaaagaata cttatatgca aacttagcat aatcgttaat
     2821 tgctttattc aaggctttaa atgatgttac tttagcacca aatatcttta taacttgaat
     2881 tccgcggata tattc
//
LOCUS       DAYYAG010000204         2872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000204 DAYYAG010000000
VERSION     DAYYAG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.209"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(279..2465)
                     /locus_tag="ACHARQ_002864"
     CDS             complement(279..2465)
                     /locus_tag="ACHARQ_002864"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8411237.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2684..>2872
                     /locus_tag="ACHARQ_002865"
     CDS             2684..>2872
                     /locus_tag="ACHARQ_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HFM8411238.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLST"
BASE COUNT          761 a          545 c          478 g         1088 t
ORIGIN      
        1 aataaaatat aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt
       61 ctatatattc tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg
      121 acaaaattct tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc
      181 gaaaataagc tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt
      241 tcttgaaact gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga
      301 ctcccggttt ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt
      361 acacatagat acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct
      421 tcttctatcg tcgttactgg ttctgctttt atttcattaa agtcgattac tttcttttct
      481 gtcgctccgt tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa
      541 ctaagacctt tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg
      601 taagcaatca agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct
      661 tggagtcttc tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc
      721 tggtcgattt ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt
      781 tcttgatgct ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc
      841 gattttaaat tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta
      901 ggagaaaagt attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg
      961 agcaattgct ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca
     1021 attttttgat agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct
     1081 gcttccagta caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt
     1141 gaagctgatc ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc
     1201 tgaatttgtt tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc
     1261 ataacacctg cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta
     1321 tagctgatca atctatttgc tccttggaca atagctgccc gagaacgata attagttaat
     1381 aagaaaagag ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat
     1441 cctctgaatc catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt
     1501 tcctttgtca tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca
     1561 atgaatagat aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg
     1621 cttttcagat tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct
     1681 tggagttccg tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca
     1741 atggaatcat atttttcttt aactaactga accatttctt ctactgaaag gttcatattc
     1801 tgccaatagt ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga
     1861 ttatatttac gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa
     1921 aagtctcctt gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat
     1981 atcctaaaaa agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa
     2041 ttatctactc gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg
     2101 cgttcttcgt ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc
     2161 gttttgccgc tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata
     2221 gctgcttttt gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa
     2281 gagctgtttc cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg
     2341 ccataagccg cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc
     2401 tttttatact tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca
     2461 ttcaactgat tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt
     2521 atttaaagtt tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat
     2581 gggaagcgat atgattttag ttttctgaaa atttttcctt ttttttcaat cgtttatgct
     2641 aagataagga agacaaaaat attcataaaa ggtgatgaaa aaaatgcaga cttcctttca
     2701 cgaggggcgt acaataggac ttttgctgac atttatcgga ggagccatgg acagttatac
     2761 ttatatccaa tatggtgctt ttgcctctgc tcaaactggc aatatcattc tatcgatcat
     2821 ccaagcattt gacggagaat ggctaagtgt tggtaaaaaa gtattgtcta cc
//
LOCUS       DAYYAG010000205         2655 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000205 DAYYAG010000000
VERSION     DAYYAG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.211"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            679..765
                     /locus_tag="ACHARQ_002866"
     CDS             679..765
                     /locus_tag="ACHARQ_002866"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8411239.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1067..1291)
                     /locus_tag="ACHARQ_002867"
     CDS             complement(1067..1291)
                     /locus_tag="ACHARQ_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411240.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1657..1941
                     /locus_tag="ACHARQ_002868"
     CDS             1657..1941
                     /locus_tag="ACHARQ_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411241.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1944..2360
                     /locus_tag="ACHARQ_002869"
     CDS             1944..2360
                     /locus_tag="ACHARQ_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411242.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2497..>2655)
                     /locus_tag="ACHARQ_002870"
     CDS             complement(2497..>2655)
                     /locus_tag="ACHARQ_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8411243.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          873 a          496 c          426 g          860 t
ORIGIN      
        1 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
       61 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      121 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      181 aaaagttgtt agtacccttc tgtatccgtt gggagagtaa ggctcaactg ccccctaaaa
      241 agggttctaa aaaatcttta aaaaataaat aaaataaacg cgcgcgcgcg agcaaaattt
      301 cccgccaaaa aatcacccac tttttaaaat aaacaggttc gcgcatgata ttaaactgcc
      361 aatctagctc cctatggtcg ctgtaagaag gagtgatcgc tacgctctca cattttactt
      421 gcagcagtcg cctttaggct ccttcgccaa aaataattca ttagggactc ctttgtggcg
      481 tcctgagagc tcgtctaagc cgttctaaga caagattcga taaaaactat gtccttcgtt
      541 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
      601 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
      661 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
      721 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
      781 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
      841 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
      901 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
      961 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt tcattggtcc
     1021 aagctaaaaa actcttagaa gcgattttag agccatttaa acccacttaa ttatattctc
     1081 catgaaattc gctccatgcg tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta
     1141 gttgcattaa tttttccaac gatcgttttc tttcgggtgt catattttta tattccaaca
     1201 atttttcgtc tgttaaatcg aacttgattg cttgaattgc ttgctctaat ttttctaagt
     1261 tttgaaaata ttcgtaagga aatttttcca ttttttcttt ctccagtctc acaatttttc
     1321 tagaaataaa atcacttctt tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact
     1381 aaataaattt ttgatcgtgc atttttaaat ttggttatcg attacacgtg cagcactccc
     1441 ccccgaattt taaagagcat aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat
     1501 aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat cactttttcg ccatcgtggg
     1561 tagcttatgt atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat
     1621 acataaatta tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt
     1681 gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga
     1741 gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa
     1801 cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa
     1861 acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatg
     1921 cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt
     1981 accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
     2041 actcggaagc cattcgcttt ttatgtatga gttatgaacc cgataccaac acgaccacta
     2101 ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca
     2161 tcgcagaatt tgctgattta tatctaaaag atacaggcgt tcttattggc acaaattcta
     2221 aagtatatca agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc
     2281 gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac ttctagaaat ttcgacccaa
     2341 caaattttct aaaacgataa aaaacagata ggagttattc ctatctgttt tttatttaga
     2401 agccccatac aatatgctag aatgtactag caatcttagt cggttgtaag ggacttgtct
     2461 acttgcttaa tgtaaggggg gcgagttctt ttttatttat tcttctaacc agttacgcag
     2521 agaaatcttt ggcaaatctg acgttaggct tgaaaaagta aattctaatt tggcgatttt
     2581 attgcccttt tttgctttta tttttgtgac tttaagatca ttgaagtatt tttttagttc
     2641 tcgtaatgca ggatt
//
LOCUS       DAYYAG010000206         2631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000206 DAYYAG010000000
VERSION     DAYYAG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.212"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..2562
                     /locus_tag="ACHARQ_002871"
     CDS             121..2562
                     /locus_tag="ACHARQ_002871"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8411244.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          886 a          461 c          616 g          668 t
ORIGIN      
        1 aaatataaaa aagagaatat ttaaggattg ttaaaattta atcttagaaa aaaataactg
       61 gaacaagagc tatttgtttt tacagcaaag aatggtatat ttttgtagga ggtggcttac
      121 ttgaaagaag tcgttatgat agatgctgca agaacgccga taggaaagta tcgcggaagt
      181 ttaagtccat ttactgcagt tgaattaggc acacttgtga ctaaaggatt attagataaa
      241 acaaaactga aaaaagataa aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt
      301 aatggtcaaa atgttgccag acagattgct ttgaacagcg gtctccctgt agatgtccca
      361 gcaatgacga tcaatgaagt ttgcggatct ggtatgaaag cagtgatctt agcacgccag
      421 ctgatccaat taggtgaagc agaacttgtg atagccggcg gaacagagag tatgtctcaa
      481 gcacctatgc tgaaacagta tcagtcagaa acaaatgaat atggtgaacc aatttccagt
      541 atggtcaacg acggattgac tgacgcattt tcaaatgcac atatgggatt aaccgcagag
      601 aatgttgcaa cacaattttc tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc
      661 cagttgaaag cagcacatgc tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc
      721 aagatttctg atgaagacgt gttatctgag gatgaagcag ttcgtggaaa tagtacattg
      781 gaaaaactgg gcacgttacg tacagtattc tcagaagaag gaactgtaac agcaggaaat
      841 gcttccccgt tgaatgacgg tgcctctgtg gtgatccttg catccaaaga atacgcagaa
      901 aataataatc tgccttattt agcaaccatc aaagaagtag cggaggtcgg tattgaccca
      961 agtatcatgg gaatcgctcc aatcaaagcc attcaaaaat tgacagatag atcaggaatg
     1021 aatttatcaa caatcgatct ttttgagatc aatgaagcat ttgcagcttc ttctatcgta
     1081 gttagccaag aactgcagtt agatgaagaa aaggtcaata tatatggagg agcaatcgca
     1141 ttaggccatc caattggtgc aagcggtgca cgtatcttga ctacattagc ttacggattg
     1201 cttcgtgaac agaagagata cggtattgct tcattatgta ttggtggagg tttaggcttg
     1261 gcagtcttgt tagaagcgaa tatggagcaa acacataagg atgtgcaaaa aaaaaagttt
     1321 tatcagctta cgccatctga acggcgcagt caattaatag aaaaaaatgt attgacacaa
     1381 gaaactgcgt tgatttttca ggaacaaaca ctttctgaag agctctccga tcacatgatc
     1441 gaaaaccaag tgagtgaagt ggaaatccca atggggatcg cgcagaattt tcaaatcaat
     1501 ggcaagaaaa aatggattcc gatggcaaca gaggaaccat ctgtcattgc agctgcaagt
     1561 aatggtgcaa aaatctgtgg aaatatctgc gcagaaacac ctcagcggct gatgcgaggg
     1621 caaatcgttc tatcaggaaa atcggagtat caagcagtga tcaatgcagt taatcatcga
     1681 aaagaagagc tgattctttg tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc
     1741 ggtgttcaag atatctctac tcgggaattc atggggagtt tccatgcata cttatctatt
     1801 gattttttag tggatgtaaa agatgcaatg ggcgctaaca tgatcaactc gattcttgag
     1861 agtgtggcaa acaagttacg ggagtggttc cctgaagaag agattctatt cagtatttta
     1921 agtaattttg ctacagaatc attggcttct gcctgttgtg aaatcccatt cgaacgctta
     1981 gggagaaaca aagaaatcgg tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat
     2041 gcgaagcttg acccttaccg tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa
     2101 gctgtagtgg cagctacagg aaatgataca cgtgcggtta gcgcttcgat ccacgcctac
     2161 gcggcaagaa atggattata ccaaggatta acagactggc agatcaaagg ggataaacta
     2221 gttggaaaac taacggtgcc tttggctgtt gccacagtag gcggagcttc caatatcctg
     2281 ccaaaagcaa aagcatcttt agctatgtta gatattgatt cagccaagga actagcacaa
     2341 gtaattgctg cagtagggtt agctcaaaac ctggctgctt tgcgtgcatt agttacagaa
     2401 ggaatccaaa aaggccacat gggacttcaa gctcgttcat tagcgatctc aattggggct
     2461 attggtgagg aaatcgagca agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag
     2521 cagacagcta tacaaatatt ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga
     2581 agaataaagc aagatttaca gttattttat ttactgcaga tcttgctttt t
//
LOCUS       DAYYAG010000207         2630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000207 DAYYAG010000000
VERSION     DAYYAG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.213"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACHARQ_002872"
     CDS             120..626
                     /locus_tag="ACHARQ_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8411245.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACHARQ_002873"
     CDS             643..1542
                     /locus_tag="ACHARQ_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8411246.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACHARQ_002874"
     CDS             1629..2513
                     /locus_tag="ACHARQ_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8411247.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          958 a          489 c          515 g          668 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat
//
LOCUS       DAYYAG010000208         2624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000208 DAYYAG010000000
VERSION     DAYYAG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.214"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2303
                     /locus_tag="ACHARQ_002875"
     CDS             <1..2303
                     /locus_tag="ACHARQ_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8411248.1"
                     /translation="NYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKES
                     SASQQSNWSGLYFNSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVS
                     EQSLMELLGQRIVEQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKK
                     GMNPTYSTEGLSVLAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEW
                     LGVERFKTNLKLETFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPC
                     SKISAKDVEQDGQSIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFK
                     QVLNLSLDLNLIELLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLL
                     SNGLNEEEYPTIFEIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFW
                     KLKLRSYLDKKAEHYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQV
                     DIEKESKKRFPELSVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSL
                     LYFGIPSRLREDILIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTR
                     NGERSKRDYDNRNSANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPN
                     GIVCDMWVHDPENGNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISR
                     HKFDELDGLKQASSYLFKFKQKRSDG"
     gene            2438..2566
                     /locus_tag="ACHARQ_002876"
     CDS             2438..2566
                     /locus_tag="ACHARQ_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8411249.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         1003 a          343 c          499 g          779 t
ORIGIN      
        1 ttaattattt aacttctaag tacatcagta attttgaaga attaatagac aaattagata
       61 cgaaagacat tgtcaattgg ctcaatcaag acgaagaatt gacagattta ttatcttcgg
      121 taaaaaaaga atcaagtgca agtcagcaat cgaactggag tggattgtac tttaattcaa
      181 atgagtatgt gaaatacatt ttgaataaaa ctgcatggat taagattgat ggagaaaaat
      241 atagcccgag acaaattgtg ctatatgaaa agctccaggc acacgttccg ggattgtatg
      301 gagtttctga acaaagctta atggaactct tggggcaacg cattgttgaa cagttggatt
      361 tcagaaaaaa tatggcgatg ttttcggatg atgaaataag agctatatta gttgagttac
      421 caaaattcga taaaggcgaa ataagtcgca gattatataa tgaacttata agaaataaaa
      481 aaggaatgaa tcctacttat tctaccgaag gattatcagt cttagccaaa gatggcttct
      541 tttatccaaa caaagaatta aggtatgctg ataagcgact gcctaaatca atagaaaaac
      601 aaatgcgttt tatttacgtg cctgaaaaag ctagtacaac tacgatatat gaatggcttg
      661 gggttgaacg cttcaaaaca aatttaaaat tggaaacatt tgagttattg gaagattatt
      721 ctgaggattt caaaaaagag attgatgata tcaaaactgc ggtattatct acgatagatg
      781 acaatagtaa gaatgttagt tcattaaaac gtattcagat tattccatgc agcaaaatat
      841 ctgctaaaga tgttgagcaa gatggtcagt caatcgtttt ggaagattat tattttgtgg
      901 aaagtaaagg aaaattctac ttaaaattac ctgctaattc tattgaaatt aatcaattac
      961 gtttgattga tacgttttct tcaacaatcg tagacatatt caagcaggtg ttaaatctaa
     1021 gcttagattt gaatttgatt gaattgttaa tttcccgaga tacggaacat aaacaaagaa
     1081 aaataactga agaattcggt gtagataagt ggaatgaatc gtttgagcta ttgtttagcg
     1141 aaaatgcgtt aaataaaaaa gttaatgact ttttgttatc aaatggacta aatgaagagg
     1201 aatatcctac catttttgaa attgactatt catatgcatt aaaagaagaa gagtatcaat
     1261 tgttggcgat agctctagaa agcatttcaa aagatattga ggatttgaat ggattgtcag
     1321 aattgattca tttggatatt cgtgaatttt ggaaactgaa attaagatct tatctagata
     1381 agaaagcgga acattaccgc cagattttat atagcaatgc aaataaaagc ggtgatgaaa
     1441 tactcaaaga aacattccta gaaaaaattg atagatacaa agattatcaa tttgagattt
     1501 ctgaaattcc aaattcagtt caagtcgaca ttgagaaaga aagtaagaag agatttccag
     1561 agctaagtgt gtcaattact gatgaaattg acattgatgg cctgtatgat aataatgtcg
     1621 aagagttagt cacggttgca gctattacaa tagatgattt tgattattat attagaggtc
     1681 atcaaaaagt ttcttcctta ctgtattttg gtattccgtc ccgtttaaga gaagatatac
     1741 tgatattcgt tactaaagaa aatgatgttg atttgaacgg agaaaaatct ggtatctcta
     1801 ttgaaggaac atcaactgtt caaaccattc ttgaaccatc gaatagtgtt agtaggaaag
     1861 gtaagaacac acgaaatgga gaaagaagta aacgagatta tgataataga aactccgcaa
     1921 atgatgatgc tggtgaaaca gcagaacaaa ttgcttacgc tgaattgact cagaatcaaa
     1981 aacttaatgt aatttggaat acgaaatatt ctagtaaatc ttcagatcgt aataagcagc
     2041 caccaaacgg aattgtttgt gatatgtggg ttcatgaccc agagaatggg aatatgtact
     2101 ttgaggtaaa atcatcaatt acagaatttg aaatgtcgat taatgaatat aattctatgt
     2161 tagataaccc tgactcttac gaggtcgttt tagtcaatcg tgataccaaa gagataagtc
     2221 gtcataaatt tgatgaactt gatggattga aacaggcaag tagctatctt ttcaaattta
     2281 agcaaaagag aagtgacggt tgagtgattt gtttccacaa acctattcaa tctatcaact
     2341 caaaaatcat aaacaaccat tattatagta ggaagataca gagtgtactc tcaacgcata
     2401 tcgaattagt cgcttcattc gttaaaggag aataaaaatg gaccaaaaag aaaaaatgaa
     2461 agaattgatc aaaaagaaac aaggtggagg ccgatccaaa caaatggaaa cacctaagaa
     2521 tgatcgtaaa aacatgagaa aaggccctaa aatctttaat aaatagacgt gctaaagaaa
     2581 gactaagaag tgttaacttt tgttattgtt tcttagtctt ttta
//
LOCUS       DAYYAG010000209         2579 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000209 DAYYAG010000000
VERSION     DAYYAG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.215"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(300..545)
                     /locus_tag="ACHARQ_002877"
     CDS             complement(300..545)
                     /locus_tag="ACHARQ_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8411250.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            650..1423
                     /locus_tag="ACHARQ_002878"
     CDS             650..1423
                     /locus_tag="ACHARQ_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411251.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(1476..1790)
                     /locus_tag="ACHARQ_002879"
     CDS             complement(1476..1790)
                     /locus_tag="ACHARQ_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411252.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT          848 a          438 c          359 g          934 t
ORIGIN      
        1 actttcaaat agattttcta ttctcttgtt aatagatttt tggttgtgat ctatttgatt
       61 tactacatgt gtatatgatt gcgagcttga ggagaatgca tttaagtcag catttgtaaa
      121 atccaattca tatgccttat acaaggatat ccctccattc ttgtattttg atccacaatt
      181 tattaggcaa cataacagta taccctatcg ttacatatcc cattcattat tataccagta
      241 tttcttcgtg acacataagg gtgtaacata ataatttcaa acgaaatgac tacaagtagt
      301 cattgaagca aatcttcata agacacctgt agaatatctt tgatggcttt caattgatcc
      361 aacttaatgt gttggcggcc accttcaatc ttaaccaacg tttccctagt tatagcaatt
      421 ttacgaagct gtaggtctct aatcagctcg gtttgaccaa ttcctttttc taatcggact
      481 cgtctaatat tgctaccgat tacattttct ttatcaataa ttttttcctc tttcaagaat
      541 agcacacctt ttggactaaa attagtcctt tttcttatta atcttaaaga ttcggtatgc
      601 tacaatggga ctagtattag tccatattca attttaagga ggaatttttt tgaagaaatt
      661 tgcaatcatt ctaacttgcg ctttactcat cggaactata agcgggtgta ccacttctga
      721 cacaagttct acaagtacgg aaactagtac aacaacctca gaaaagaatt acccaataca
      781 tttttttccg gttccagatg gatttgaagc ttattatcat gataaaaaac tagatgttcc
      841 tttatcagaa actgaatcta ctgaaaagat tttgccaatt gaaattgaaa gaaatcaaga
      901 cgatataaaa ctaaaagctg atcttccctt actgaacagc acacaaattg atagacatga
      961 aaatattaaa gttaatattg aaaaaggagc gttagcacaa ctttatactg aaaaagttga
     1021 acaatttttt gaggctggtg ccaataaaga cttttcaaaa ataagcgatt attcggataa
     1081 cttttttaat gagacccaag atgaaatcac gaactcaact tttatggctg aagatatgta
     1141 caatatgaag ttgaaaaaaa taaagtcgta tgattatcca ttaaatctaa cattaaataa
     1201 agggatactc tcttttgtat tagcaggtta cgttgactat gaatatacag cagattattt
     1261 ggagacgccc cttcaacacg ataataacga tattaatttt agatttatct acaattcaaa
     1321 aagtaaaaca tggttagttg atcaattgag cactataaat acgggatttc atgaattacc
     1381 taagcctgac aatgaaactg ctttggtcat taaaacattc taaaaaagaa acgttgaagg
     1441 aataattatt ctttcaacgt ttcttttatt gtttttcagt ttacggtctt acctgagcga
     1501 ataatctgat ttctaaatga ttggaaaact tcttgtggtt gataataatc tgtatctatt
     1561 tcaaatgttt ctgagaataa ttcattgaat ttttctactg aaatgatgat tttaccattc
     1621 tcctctcgac tgttttctat tatttctgcc tctaattcaa ataaatctag tgacgaacta
     1681 aaatcataaa tcctgtcata ttgaacaaaa aaatcaagaa aaagttgctc aaactcttcc
     1741 ttatttatca ctagtgtttc aagcggaaca actttatttt catcagccat tcttccaatt
     1801 tcctcacttt cattttcaat tgtccattgt tgcggacgta tcagattttc tctatcattt
     1861 ttcagtgtat agatcacttg taatacacaa caatgctttg taaacttatt tttttagtta
     1921 gtcgaatctt tttaaatgtg cgtttcatac catgcgcttg atatattttc tcctattttt
     1981 taaatcgttt atcacaactt ttatcgtttt ttcttctgaa aaaaatcttg tttacatcgt
     2041 tacatagttt ccaaaaagat acattactag tagaaacaac aagattaatt ttacttccca
     2101 ctgaaatggg aagtatattt taaatatttt ttctccttca acttacacta taaagtatct
     2161 taatcacacg atatgctagc cactaaattc atcatatttg attccttttt taagataatg
     2221 tagcatcttt ttatccaata acgcaattgt ttttaaggat aaatttctta agatggtaca
     2281 tttcaaaatg gcatttgaca tatgaaacaa aaaagaaaga aaagagcata agggaaaact
     2341 gatagcacct tcctacagga aggtggctaa gttggctgtg ccaactggtt ttctttcaaa
     2401 atcatttcat aatttttgat atcataaaaa aatccatttt ggacctattt tcggtcataa
     2461 tttagtacct acttttggtc atagtttcgt ccgtagtttt ggtcatagtg actaaggctt
     2521 ttctgttcac aaaattggtt taaatttgct ttctttaaca cttcatttat atcatagca
//
LOCUS       DAYYAG010000210         2576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000210 DAYYAG010000000
VERSION     DAYYAG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.216"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHARQ_002880"
     CDS             <1..1412
                     /locus_tag="ACHARQ_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8411253.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2576
                     /locus_tag="ACHARQ_002881"
     CDS             1546..>2576
                     /locus_tag="ACHARQ_002881"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8411254.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLN"
BASE COUNT         1009 a          360 c          518 g          689 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaattt
//
LOCUS       DAYYAG010000211         2543 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000211 DAYYAG010000000
VERSION     DAYYAG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.217"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..456
                     /locus_tag="ACHARQ_002882"
     CDS             <1..456
                     /locus_tag="ACHARQ_002882"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411255.1"
                     /translation="NLRECINEGLIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVK
                     FITHDGGVWVLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTL
                     VNKDIPNNSVVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            544..1710
                     /locus_tag="ACHARQ_002883"
     CDS             544..1710
                     /locus_tag="ACHARQ_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8411256.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT          888 a          337 c          494 g          824 t
ORIGIN      
        1 aatctgcgag aatgtattaa tgaaggtttg atattcggaa aaaaacatag tttagtagag
       61 gttcctgaat ttgggagcga gccatattta attgaaatag gggataattt cagatgttca
      121 tttggtgtta aatttataac acacgatggt ggagtctggg tcttaaggaa gatgagttct
      181 gtgagcgagt caacttcaaa aataggtagg atcaaattgg gaaataatat ttttattggt
      241 atttactcta ttgttttccc aggagtttca attggcgata atgtaattat tagagcgaac
      301 actttagtaa ataaagatat cccaaataat agtgttgtag cgggtagttc atgtaaagtg
      361 atttcaacta tagaggagca ttatgagaaa aataaaaatg aatttattaa atatcgaacc
      421 tcgatgattt tgaagcaaaa aaattttttc tattaaataa tattgattga ataaggaata
      481 tgttcaaaag gataacagct aaaatactat aaaatgaaat ttgattggag cgtatggaag
      541 aatatgaaaa tttcagtttt cggattaggc tatgttggtc tagcaaattc actattatta
      601 gcacaaaaag agcaggtcaa agcttatgat atcgtggaag aaaaaatcac gatgttacaa
      661 aatggcatct caccaataga agataaagaa gttcatgaat ttttaaaacg ggatgactta
      721 aatgtcgaat ttacgtcaaa ttttactgat gcagtaaact tcggtgatta cttgattatt
      781 gcaactccaa cagattatga cgaaaagaaa aactatttta atacatcaac agtagaagat
      841 gtaattgaga aggctttagc aattcgtcca gattcaacat tcattattaa gtcgacagtt
      901 ccagttggct atgcaaatga aattaaagcg aaatttggta cagaaaacat cattttttca
      961 cctgaatttt tgcgtgaagg aaaagctttg tacgacaact tatatccatc aagaattata
     1021 gttggagaat gttctgctcg tggtcgcgag attgcaacta tgtttcaaag aaacgcattt
     1081 aacaaagatg catcagttct tttgactcac tcaactgaag cagaagctat taaattattc
     1141 tcgaatacat atttggcatt gcgagtggcg tatttcaatg aattagatac gtatgcagaa
     1201 tttcgtgggc ttgatagtaa gcaaattatt gaaggtgtcg gtttggatcc acggatcggt
     1261 tcgcattata ataatccttc ttttggctat ggtggatact gtttacccaa agacacaaaa
     1321 caattaagag ccaactatga aaaagtacca agcaatatca tcagtgcaat tgttgatgca
     1381 aatacaacaa ggaaagattt tattgctgga cagattttgg cgaaaaaacc aaatgttgtt
     1441 ggaatctatc gtttaacgat gaaggccggt tcagataatt ttcgatattc gtcgatccaa
     1501 ggaattatga aacggattaa agccaaagga attgaagtgg ttgtttacga gcctacattg
     1561 gatgaagatg aattttatca ttcacaagta ttgaaagata ttaatgaatt taaaaatatt
     1621 tctgatgtga tcgtatctaa tcggtttgaa gatattttaa aggatgttga agaaaaagtt
     1681 tataccagag atatctttgg tgataactaa ataataatga acgggtacat ggaggaaaat
     1741 attttagatg gtgactcgaa ataaatgttt gttctcattg catctgtatc tataccgata
     1801 agtttaaaaa ttccgattag gaaaatgaag ttggctatcc ttgcgaggaa tttattgctg
     1861 gattatttta tgaactaaag caagacatca tcagaaaaaa atccaaaatt tgacaatggg
     1921 attgatcgtt taccagctgt aagcgaagta tctgacacga aaatggaaaa tgcaattact
     1981 gttattacca atagcttttt ttcatcgatc aagtatttga aaacattatt tgtttgccaa
     2041 gctgatcaat gttctttcag acttggaaga acattgatca gctcacaagt aatgattgaa
     2101 gtttaggtta tgggataaag gataagtgaa ataaaagtcg tgtctgcatc tttgttagta
     2161 ccagtaaatt tttctactac ggcgctagtc acgacagtcc aggcatctga aattgaagat
     2221 gcaaatagag tttctgctag tttaggtatt gatgttactg tattagcatt aatcttagag
     2281 ccagtagcat ccaataggtg tagctatagt tctaatagta taaaacaatg cagaggatat
     2341 gattgcttta tgtctcggtg tgtcaggtca aaatagagtt ccatatgggg cgatttctca
     2401 atgaataatc ttatagatat aatcttgttt gctatttcgt tatcactttt acttttcttc
     2461 gattatttca aaaataaaaa tccaaaatat tttaagttct tactatttat cattactgta
     2521 ctagctgttt tatcgatgac aga
//
LOCUS       DAYYAG010000212         2520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000212 DAYYAG010000000
VERSION     DAYYAG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.218"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..686
                     /locus_tag="ACHARQ_002884"
     CDS             3..686
                     /locus_tag="ACHARQ_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8411257.1"
                     /translation="MYIQNRPASVSASRVIELREREGNDSIIIDKGYYKNVTRKIECY
                     YKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQAVVIEGPEFKGTRKTGNIV
                     PFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKPLIKLSGSGDASFYINKDKY
                     DLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFPFLYPGKNEIKWTKNIHSFE
                     IMPRWWRKV"
     gene            683..>2520
                     /locus_tag="ACHARQ_002885"
     CDS             683..>2520
                     /locus_tag="ACHARQ_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8411258.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          932 a          306 c          498 g          784 t
ORIGIN      
        1 atgtgtatat tcaaaacagg cctgcttctg tttcagctag tcgagtaatt gaattgagag
       61 agcgagaagg aaatgattct atcattattg ataaaggcta ttacaaaaat gtgactagaa
      121 agattgagtg ttactataaa gcaccttcaa ttgatatggt gcaggagtgg gaagatcgaa
      181 tcactgaatg gttagatatg tcgtcttata gtgattttat tctttactat gatgaacaat
      241 atatttatca agctgtagta atagaaggac ctgaatttaa aggaacaaga aaaacaggaa
      301 atattgtccc ttttgaattt acagtgagta ttcgaccatt taaggaaaat tacaatggta
      361 gatttactat tcgacaaaca gaaacttttg aaatttataa cccagagaag tattcttcaa
      421 aaccgcttat taaattgagt ggttctggag atgcttcttt ttatattaat aaggataaat
      481 atgatttgaa atcgttggac agagaattat ttatagattc taaattagaa gaggcttatc
      541 gaaaattaga tggtaattta gaacatcagg atcaggtcac cttattttta gattttccat
      601 ttttatatcc tggaaaaaat gaaattaaat ggacgaaaaa tattcattca tttgaaataa
      661 tgcctaggtg gtggagaaaa gtatgaaacc aagaatatat agtcctactg aaacagattt
      721 ctcaacgaat ggcttaggaa ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc
      781 taatggaaaa tatgaattag agttggatta tcctttagga actagatttg atgaatattt
      841 tgaaaatgac tatcaaataa aagcaaagcc aaacgatcaa gaagagtatc atatcttttt
      901 tattgatgat aaagatatag atactttttt aaatacagta actatttatg ctcagagtcg
      961 tacaaatcga cttggaagac gggtagtcac tcttgtggaa gtggactcta aaactggtca
     1021 agaagcgata tcaattattg aaactaaaat ggataaaaaa tctgacatac gactttattc
     1081 tgatattacg gctgtttcta gcacaatctt tgaagcgaga aatgttttaa attgtattgc
     1141 tggtgaacaa ggatcattgc ttcaatattg gggcggagaa attaaacgtg aaccatttaa
     1201 gctttctttg ttaaagcgaa gaggtcgcaa taatgttgga acgattcggt atggaaaaga
     1261 tatgtctggt ttgaaggtca aattagattg gacaggtata aaaacaagaa ttattccata
     1321 tgctgatcct caaagtgaag taggtacgac taatcgaatt tatggttcgc cagtagatag
     1381 tgcctatatt aataactatc ctgatgtcta tacagaacat gttcagttta cagaagaaca
     1441 aggagtaaaa gacattaaga gcttaaataa aatagctaaa aattacttta aaacgatcaa
     1501 tcctggctgt gataagccta aagtttctat tacggttgaa tttgataagt tgactgatag
     1561 tgaagaagcg aaagaatttg cgaagattag aaattatggt ctgtttgata catttaaaat
     1621 atatcataaa aaatataata tttatcttga atcaaaggtt agtggactac aatacgattc
     1681 tttatctgaa aaaactttga aattagaagc aggagacatt caagttgctt tttatcagca
     1741 acaagctgta actattcagg ataaattaaa agattatgca acgaataatt atatgagtga
     1801 ttttaatgat tatgtttctt caatgattac aggtcaaggc agtgcaggag gatatgtagt
     1861 tttatggcca aaagaaaaac cttccaacat ctttattatg gataatccag atttagaaaa
     1921 ggcaagagaa gtactaagaa tgaataaaaa tgggattgct ttttctaaga aaggatggcg
     1981 ggggcctttt aattcagctt ggacattaga tagtatattt aatgccaatt ttatacaaac
     2041 tggattaatc aaagcagata tctttcaaaa ttcttttaac aagactggag atgtattgaa
     2101 attagttaat ggactactcc aaatttggaa caacaaaaag aaaataatgg agttaactaa
     2161 aaaaggaatg gaattttgga attctaaggg ttcaattgga acgattggaa caactgattc
     2221 tgttggtaat ccttttcctg gggcttctac tccaacccct attgaagata attctttagt
     2281 tattcgtaca aatggagacg gcaaatatat tttgatttct cctaaagtag gaaaaggatt
     2341 agttttatta ggaaatggta aagcaattta ttttggagac ttagatgtac aaggaaaact
     2401 tacagttaaa ggaaaagaaa taattcctgg acaaggtggt ggtccatcgg gaggtggaga
     2461 atctactggt gggtatccta gtgaattaaa aacagatgca gaaaagagag cttggagaat
//
LOCUS       DAYYAG010000213         2517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000213 DAYYAG010000000
VERSION     DAYYAG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.219"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1356
                     /locus_tag="ACHARQ_002886"
     CDS             <1..1356
                     /locus_tag="ACHARQ_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411259.1"
                     /translation="GEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIP
                     AVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKN
                     DIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQA
                     LNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKT
                     PISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKEN
                     YAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEK
                     GKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAG
                     ENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNEN
                     K"
     gene            1343..>2517
                     /locus_tag="ACHARQ_002887"
     CDS             1343..>2517
                     /locus_tag="ACHARQ_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8411260.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNF"
BASE COUNT          843 a          378 c          425 g          871 t
ORIGIN      
        1 ggagaaaagt tttttaaaaa gatagatgct gcttttttat ttagttgttt atatactttt
       61 gcaacttatc gaacagttga tatctattat cgatccgcaa ttgcagaagc aatcgctctt
      121 acgattatcc cagctgtttt atattatgct tatggattga tattcaatag agaaaaaaaa
      181 gtcatcgctt tatctgtcag tataagtcta cttgtgtata ctcatgtctt aactacacta
      241 ttgtgtagtg cattgattgc ttgtatgatt atagttcgtt tgtttttaag caagcaaata
      301 aaaaacgata taaaatttgt ggtcgtgaat ttagtaaagg ctgttttttt gacttgtgca
      361 ttaactagct atttttggta tcctatgttg cagcagatga attatcaaga aatacacagt
      421 cccttcaaaa cagatttgca gctagaagca ctttctattt cagatagtat cattcaagcg
      481 ttaaataacg atattacaac atacactatg gggttattag gattggttgc gttgattttt
      541 cctttattca tactgaaaga aatggagaaa aaagaaaaaa tcatctatgt tggcgtagtt
      601 tctacatggg tatgcgtaac taatttattt ccatggtttt tgtttcaaaa aacaccgata
      661 agcttactcc agtttccttg gcgtatctta ggtatccaaa tctttttttc ttcaatgatc
      721 ataacgcttg tttttatgaa aaagactatg aataaaaaat attcatggtt aatcattggc
      781 gctactgtgg tgtttctact cgtcacgtct atagcagcaa aagaaaatta cgcaaaagtt
      841 atccaatcaa aacacggtca tattgttatc aatgaagaaa cactcccacg ttatacaaca
      901 tcagatatgg gaggattata cgattacgcc ccaacagaag cgataaaaga aaggcctcac
      961 ttagaaaaac atgaagtaaa agtaggagaa tcgtgggaaa aagggaagta taaagctgca
     1021 gataataaca ttacttacac agtggcaagc aacaaaaagc aaaaagtgac agtaccagta
     1081 tatgcttatt taggtacaca ggtaaaagta aacggtaaag tcgtaaatga ctcttataaa
     1141 aaacatggct tagtgaatgt ggatgtaaat gctggagaaa ataagataga aattacaaca
     1201 aaatatactc caactgctct catggcttta tggatcagta ttctgactta ttgtttagtg
     1261 gtgtattcaa gttttaaacg atttgcacct aataagttga aagattctcg atatgtgact
     1321 aaaaaggaga taaacaagaa gaatgaaaat aaataaaaaa gtagtaatat tgagtggatt
     1381 aattatagtt agtagtatat attctttcat ctttttcaaa aatacacaga gtatttatac
     1441 cactggagat ctttcttttc acttatctcg aatcaagggg ttatcgacta tttttaccag
     1501 tccgattaat tatgagacat tcaattatac tggatatggt gtaaattatt tttatccctt
     1561 tctgactttt tttccagctg taatgttata ttggatgaca aaaaatttga ttgtaagcta
     1621 tattatttac gtttggttat tgaacttgtg tacaacactc gtagcttatc attatggcga
     1681 gcggttttta aaacaaaaaa aagcggcatt tttatttagt tgtctctata ttttctctgc
     1741 ttatcggaca gtagacatct actatcgttc cgcaattgcc gaagctatcg caataacact
     1801 tgttatccct gtattatttt atgcttatca aatcatctct ggaaaagaag aaaagtatcc
     1861 gtctgtaaag ctagcattga gtatgagttt gctagtgtac agccatgtac tatcaacatt
     1921 aatgagtaca gcattaatta tcatttttat ttttattcgt ttggtttcta aaggtttcaa
     1981 gaatgcagat tttattgcta tatttaaaaa attatttagt gcggcaggaa tgacattggt
     2041 tctgacgagt gctttttggt atccgatgtt tgaacaaatg ctctaccaaa aaatcaacaa
     2101 acctagcgtg acaaatttat atgctcatgc atctaatgtg ttcgatagtc tgactgaagc
     2161 tatgaacaac gacttaacta cttactcaat gggactagtt ggtctgttat ctttatgtat
     2221 tcccctgatt ttatttaaaa aattaacaaa catagaaaaa aagatttatt atggtacgtg
     2281 tttgacttgg ctagctacaa ctagtttggt gccatggtat cttctgcaaa atacgccagc
     2341 caaattgtta caatttcctt ggcggatctt aagcttgcaa ataatattct cttctctgat
     2401 attaacgatg atatttttca aaaatagaag atacaataag acaagagaac tattttattt
     2461 atttatgact gttttgttat taatcacttt aactgtttct agtaaaaaaa atttcaa
//
LOCUS       DAYYAG010000214         2493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000214 DAYYAG010000000
VERSION     DAYYAG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.220"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..802
                     /locus_tag="ACHARQ_002888"
     CDS             <1..802
                     /locus_tag="ACHARQ_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8411261.1"
                     /translation="SLFESEGFDIVIHLAAQAGVRYSIENPYAYIDSNIIGFLNILEA
                     CRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSNELMAHTYSHLYK
                     IPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERDFTYIDDIVEGIT
                     RLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVETIEKYLGVKANKI
                     FLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYYLS"
     gene            1170..>2493
                     /locus_tag="ACHARQ_002889"
     CDS             1170..>2493
                     /locus_tag="ACHARQ_002889"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8411262.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNL"
BASE COUNT          784 a          355 c          445 g          909 t
ORIGIN      
        1 ttctctattt gaaagtgaag gttttgacat agtaattcat ttagctgctc aagcaggtgt
       61 tagatattct attgaaaacc cttatgctta tattgattct aatattatcg gttttcttaa
      121 tattcttgaa gcctgccgaa acttccctgt aaagcatcta ttatatgcat catctagttc
      181 agtatatggt gggaacacga aagtaccgtt ttcaacagaa gattcggtgg atcatccagt
      241 tagcctttat gcggctacca aaaaatcgaa tgaattaatg gctcatactt atagccacct
      301 ttataaaata ccaacaactg ggctgagatt tttcactgtc tatggtccat ttgggcgacc
      361 agatatggcc tatttctctt ttaccaagaa tattttatct ggaaaagaaa ttaagatttt
      421 taaccatgga aagatggaaa gagatttcac ttacatcgat gatatcgtag agggaatcac
      481 tagattaatc aataaagtac ctgaacccaa ctccgactgg acagacatcg atgatttgag
      541 tagtgaaagt tgggcaccat ataagatata caatattgga aataataagc cggttgcatt
      601 gttggagttt gttgaaacaa ttgaaaagta tttaggtgtt aaagcgaaca agattttttt
      661 ggacatgcag ccgggtgatg tcgttagaac ttatgccgat attgaagatt tagaggaaaa
      721 aatcaacttt aaaccgaata taaccttgga tgagggcatt aaaaaattcg ttgaatggta
      781 tacagtgtat tatttgagtt aattttgaaa ggaatattga agatgggtaa ttcatacgat
      841 ttttttagct ttgttaggta ttttaggaaa ttctactttc aaacgtgacg aactttctcg
      901 ccgaatagat attttctata actaaaagaa tttctataaa acaaaattgt gtatcaagat
      961 gtgtgaactg gattctgact gccaatattg attgatgaga ttgaagaaat atatagttga
     1021 tcatggatat ctttatttga aaagatctta gctattcaca tattcaaaaa aatttttact
     1081 acgaggactc acacgataca gaggaattta ggtagttcta attaaaccag aaagtcatct
     1141 atagatgtga gttgagggag gtcatacggt tggatagtag cagtaataaa tcacattcaa
     1201 tagctaaaaa agcttttatt tacgcaatag gaacattatc atcaaaaatg atatctgtca
     1261 ttataatccc tatctatgca gtatatattt ctgttgccga tttaggaagc tttgattatc
     1321 aaaatacaat tgctcaattt ctttctccag ttatttttgg agcaatatgg gaagcaattt
     1381 tacgcatggg attaggcaaa gaaaaaaata ttcagaaaaa agtagtgacg aacagtattt
     1441 gttttgttat agcaaataca gtgatattag ccattttttt accaataata tatagaatga
     1501 ttttcccgaa tttagagtat tatcaattgt ttgtttttca attaattttc actcctatcc
     1561 taagtgtttt tcaatatatt gtaagagcac taggtgaatc aaagattttt gtgatgagtg
     1621 gtataatcgc tgcgggtatc aacctgttac tccttactat ttttgttgtt acaatgggta
     1681 tggggatact tggtctctta ctctctatca taatttctaa tgttataaat ttgatttttt
     1741 tgattttcag ggggcagatc tttcaatatt tcgcaacgag ttcttttgat tttttatttt
     1801 taaagtccat gctgaaatac tctatcccac tcactttgaa tctcactttt ggatggctaa
     1861 tagttggttt ttcaagattt tatattggaa cgtacatcgg agtaacagaa aatggtattt
     1921 tttcttttgc gatgaaattt tcatctataa ttttaactct tacaagtatt gttagtatgt
     1981 ctatgataga ggatgcggtt atcacgtctg agaatgatga tttcatcgaa agattcgttg
     2041 agaaaaatac ctatattttt aaaatgtaca ttaggttagc gactctttct attcctatca
     2101 tttgctgtgc atactctttt ataaccaacc ctgattataa aaaatcatta gttctagttc
     2161 caatattagt tattatggca tgtttcagtg ggatgacaac aaatatcgga aatatatttg
     2221 cagtatacaa taaaacaaat cagttattaa tgatttcagt agtgtcaggt ctattgagtg
     2281 ttgttggcac ctatttatta ggctattctt ttggagtaat tggtgtctca tttgctactt
     2341 tgctcggtac tttagtatta tttggactta gatattattt tggaaggaaa attgcttatt
     2401 atgcatttga ttggaagcga atatcgctac ttttagttct ctattccgca atcgcttttg
     2461 tatgcattca tagaggcttc tattttaatc ttt
//
LOCUS       DAYYAG010000215         2462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000215 DAYYAG010000000
VERSION     DAYYAG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.221"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..687
                     /locus_tag="ACHARQ_002890"
     CDS             121..687
                     /locus_tag="ACHARQ_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8411263.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            832..919
                     /locus_tag="ACHARQ_002891"
     tRNA            832..919
                     /locus_tag="ACHARQ_002891"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:864..866,aa:Ser,seq:cga)
     gene            1255..2229
                     /gene="bsh"
                     /locus_tag="ACHARQ_002892"
     CDS             1255..2229
                     /gene="bsh"
                     /locus_tag="ACHARQ_002892"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8411264.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          850 a          369 c          472 g          771 t
ORIGIN      
        1 gttaaagaag actgaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa
       61 tgctaaaaat agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta
      121 atgagaagaa aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca
      181 aaggaagggt ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca
      241 cagccgattt atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct
      301 atctttgaac aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt
      361 gatctcggtt tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg
      421 tacattgatg aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt
      481 gaaatagtta aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg
      541 catactggta catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg
      601 catccaacag aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca
      661 ttagataatc ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc
      721 gttggctgca aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct
      781 attagaaata ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg
      841 gcagagtggt aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt
      901 tcaaatcctg taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc
      961 aagtaataag aacggccaaa caaaaatagc ttttcctatt ttttcgtttg gttgggctta
     1021 ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact
     1081 tttgtcacag ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag
     1141 tactattttt tataaatgct agtaaatcaa tcttctttgg cctttttttt gataaaaaaa
     1201 aatactagat gtaagataaa agtgtaaaaa cgatttgttt taggagggaa aacgatgtgt
     1261 accgcaatca cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg
     1321 tcttataatg aagtagtcac tattactcca agaaattatc gtttcgattt tagaaaggta
     1381 aaaaatttag ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca
     1441 ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc
     1501 aatgctgatt ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt
     1561 ccatggatac tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc
     1621 aatctagtca atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta
     1681 gctgataaag aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat
     1741 aatcctgtcg gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac
     1801 aattatcgct cgctatcaaa tggaacacca gaaaatcatt tttcaaacca gatcagcttg
     1861 aatgtttata gtcgtgggat gggaggattg ggcttacctg gagatctctc ttctgtttct
     1921 cgatttgtaa aagccacatt tactaaaatg aatgctgcat caggtgattc agaatctgaa
     1981 agtatcagtc aattttttca tatactaggc tcagtcgagc aacaaaaagg agtatgcgat
     2041 actggagaag gaaaatacga atatacgatt tattcttctt gctgtaacgt tgataaaggg
     2101 atttattatt atcgtacgta tgaagacagt caaattactg ctgtaaatat aaataaagaa
     2161 gacttggatc gtcatgaatt ggtcagttat ccagtcataa aagagcaaca gataaactat
     2221 gtcaattaat taatgaatag atccgtttgg tatcatattt tctgatacag tcataattag
     2281 acacgatctt taggtcgtgt ctttttttgc tgtttttttt ggaaatagca tccactaata
     2341 tttttttaac acgaagagaa taaggtagcc tcaaaaaaga aaacatggta aattagatat
     2401 tgggacttta ttttctgtaa aaacagaaac tttcatcttt tgttttcacg tgacgaaatt
     2461 gt
//
LOCUS       DAYYAG010000216         2452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000216 DAYYAG010000000
VERSION     DAYYAG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.222"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..504
                     /locus_tag="ACHARQ_002893"
     CDS             22..504
                     /locus_tag="ACHARQ_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8411265.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            541..1551
                     /locus_tag="ACHARQ_002894"
     CDS             541..1551
                     /locus_tag="ACHARQ_002894"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8411266.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1666..2379)
                     /locus_tag="ACHARQ_002895"
     CDS             complement(1666..2379)
                     /locus_tag="ACHARQ_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411267.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          833 a          403 c          500 g          716 t
ORIGIN      
        1 aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca cgaaaatata
       61 tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt ggtaataaag
      121 aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga taaaaaagat
      181 aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc attagggaag
      241 atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca aacgggacaa
      301 aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat tattgatgaa
      361 gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca aacgatggtc
      421 atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat atttttgatg
      481 ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag gtgaagcaac
      541 atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc accatcctca
      601 ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat ggaagaaaaa
      661 tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa atcaggtcca
      721 gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat ccaaatgatc
      781 tttccgcctt ggggaggaga attattgatt gagattctcg attttgtcga ttttgatgct
      841 ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact tgctgtcaca
      901 ttaaaaacag ggatagccac tgcacatgga accaacctta ttgatttaag aggagagtac
      961 ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg agaatcgatt
     1021 ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc atgagcattc atcatcttct
     1081 gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc agtaaggatg
     1141 aagggacgat tattaggtgg ctgtattgat acgatcagtc atttaatcgg taccccttat
     1201 ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt gtggtttcta
     1261 gaaaattgtg agctatcgtc agttgattta agaagatcac ttgttcagat gaaactggca
     1321 ggatggtttg atggcagctc ggggatcatg ttcggtagaa gtgcagcaga ggatttgtta
     1381 gattataaaa tgaaggatgt atatgaagat ctgtcaaagg aattaaaact accgatcttg
     1441 tatgatattg attgtggtca tgtaccgcca caactgacat tgatcaatgg tgcctatgca
     1501 gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta aaaaattttt
     1561 tcctgccttg gagtttatca aagaccaaca aatctaaata gaaaagaaaa tgaaagaagc
     1621 tgacagtatt ttgccagctt ctttttgtat gatctattag aaattttaat tatccataac
     1681 tatcacattg atcagctgtt tcacatcatt tggattacct gtaggaatag ctactacaga
     1741 tgcattatca ataatttctt gagttgtttc tctaatgaac gctggataag atgtttgatc
     1801 tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt catggttact
     1861 tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca catctttcgg
     1921 atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat aatgaataac
     1981 atgtagttcg aaatcacccg atgtatctct aaaagtatta cctctaattg ctagccaaac
     2041 acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg actgagaaga
     2101 ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt gtctcaactg tactactaga
     2161 tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat tcgtttcagt
     2221 agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtttga ttgaactttt
     2281 gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc ctacaaggaa
     2341 taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct cattattttt
     2401 ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt ct
//
LOCUS       DAYYAG010000217         2374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000217 DAYYAG010000000
VERSION     DAYYAG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.223"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..2224
                     /locus_tag="ACHARQ_002896"
     CDS             71..2224
                     /locus_tag="ACHARQ_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8411268.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          812 a          417 c          528 g          617 t
ORIGIN      
        1 ttttttcttt tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga
       61 aggataggaa atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa
      121 aataagaaaa ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt
      181 tcgcttaaat tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt
      241 gggttatttg cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac
      301 gattacggtc caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt
      361 agttacgaat aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa
      421 agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa
      481 tctgacagat agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc
      541 cacaaaaaga ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat
      601 ggtcaataaa gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac
      661 catctttaaa cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg
      721 tgttactgat caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc
      781 gactggcaca gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg
      841 cagtgtgacg actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa
      901 aggttattct cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt
      961 cctgcaagga accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa
     1021 tcaaaaagaa atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga
     1081 attccaatca aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg
     1141 caaagcacag tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt
     1201 gctagcaatg actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt
     1261 aggcacgatt actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc
     1321 aggctgggca agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct
     1381 gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc
     1441 agtcaaagca tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt
     1501 aggactagac tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta
     1561 tcagcaattg agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt
     1621 gccgaatgaa tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga
     1681 taatggcaaa gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga
     1741 cacatataca ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg
     1801 agtacagccg cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga
     1861 agtgaagaaa gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat
     1921 ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc
     1981 caaaccatta gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt
     2041 tgcggaaggt catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac
     2101 agacaatccg gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca
     2161 cataaacttg acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa
     2221 ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa
     2281 atagcttctc acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac
     2341 cgtttactcg gcaaaaaagg ttgtgacaaa aatt
//
LOCUS       DAYYAG010000218         2325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000218 DAYYAG010000000
VERSION     DAYYAG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.224"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..249
                     /locus_tag="ACHARQ_002897"
     CDS             <1..249
                     /locus_tag="ACHARQ_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8411269.1"
                     /translation="VINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLK
                     NVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            222..899
                     /locus_tag="ACHARQ_002898"
     CDS             222..899
                     /locus_tag="ACHARQ_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8411270.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            850..2031
                     /locus_tag="ACHARQ_002899"
     CDS             850..2031
                     /locus_tag="ACHARQ_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8411271.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2044..>2325
                     /locus_tag="ACHARQ_002900"
     CDS             2044..>2325
                     /locus_tag="ACHARQ_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8411272.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINL"
BASE COUNT          860 a          300 c          465 g          700 t
ORIGIN      
        1 gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat tataaatgca
       61 aatctaaatg aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc tcagtttata
      121 aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag aagaataaca
      181 aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca aaaacaaata
      241 gcttattaaa atatactagt gatccataca ttattattgg ggctggcgga cacgcaaaag
      301 tcattattga aaccttaaga ttagaaaata agttaatata tggatttttt gatgataagc
      361 aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat agctgggcgg
      421 atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta gtaagaaaaa
      481 agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt catccaagtg
      541 ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg aatgctgtaa
      601 ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc attgttgagc
      661 atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta gcgggtggag
      721 tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag atgaagaaaa
      781 ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt ccctcacatt
      841 ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt gaattctaga
      901 attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc taaggctttt
      961 aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga aagtattaca
     1021 aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc tgccatacat
     1081 cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc ttcatttact
     1141 tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt cattgactca
     1201 gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga agaagctgaa
     1261 agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca aatggctgat
     1321 tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga agatgcagcg
     1381 gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac aattgggatc
     1441 tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct agtgtctagt
     1501 gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga tcaagcaccg
     1561 tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc tgccggtatc
     1621 ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga aattttcaat
     1681 cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga agtggatggc
     1741 atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc tattgatcaa
     1801 gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc tttgtggaaa
     1861 ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga taataatctt
     1921 agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag taatatgaca
     1981 gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta ggtgtgatat
     2041 tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact tagactcatt
     2101 caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc gagacgaaag
     2161 gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc aaaaaatttg
     2221 tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt tattataaaa
     2281 gaggctttaa atttagattg taatatcttt acggaaatca attta
//
LOCUS       DAYYAG010000219         2313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000219 DAYYAG010000000
VERSION     DAYYAG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.225"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHARQ_002901"
     CDS             191..445
                     /locus_tag="ACHARQ_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8411273.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHARQ_002902"
     CDS             446..763
                     /locus_tag="ACHARQ_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8411274.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACHARQ_002903"
     CDS             1322..2257
                     /locus_tag="ACHARQ_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411275.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          846 a          313 c          434 g          720 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct
     2281 aaacatgttg gtaaatcaac aaaaaaacgc ata
//
LOCUS       DAYYAG010000220         2303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000220 DAYYAG010000000
VERSION     DAYYAG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.226"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..1972
                     /locus_tag="ACHARQ_002904"
     CDS             110..1972
                     /locus_tag="ACHARQ_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8411276.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1972..>2303
                     /locus_tag="ACHARQ_002905"
     CDS             1972..>2303
                     /locus_tag="ACHARQ_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8411277.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          827 a          342 c          453 g          681 t
ORIGIN      
        1 tctgtaatat tagttaaaaa gctataagtt gcacttagtt aagtgcaaac atttcatatt
       61 gtcgttagtt tagaaaacgc ttatattagc aatgtaagga gagatgataa tgcagcagct
      121 aacaaaaaga gaaaggcagc ttttgattta cttggtggaa atgtctgaac cggtaacaat
      181 caaaaagttg gctgaaaaag aaaatgttag tgttcggacg ataaaatacg atctagatga
      241 tataagagag tggctgaatg agcggaagca agtcctatat agcaaacgta gtcagggaat
      301 ttggatgaat attagtagct ctgatcgaat caaattaaaa agcgaattga tggatgttaa
      361 tcgttttgaa atatttgcag atcaaagtct ccgaatcaaa cgtttactaa ttatgctttt
      421 agtaacaaaa gaagctatta ctggaatgaa aatggccaac gcattagatg tcagcaaaga
      481 tacgatcatg aatgatttag atgtgctgga aaataatttt cttaaggaag gattgacctt
      541 gaatcgtcag gcgcgaaagg gtttttggat cataggagaa gaacgtctag ttcgattaac
      601 gattgaagag attcttcaaa aagagttcac ggattatgac atttataaat taatgtcatt
      661 gttattgaat ggcggtgaga ctgaatactt tgaaatgtat tctgctactg ctacacctat
      721 ccaagaggta tttaatcaag taatcattcg aatgcgacac ttactggaat ttgaaaatct
      781 agagaaactg aattacgcgg aattactaaa tattctgatt cgagtgacaa ttgctaccgt
      841 tcgcttacgc aaagaggcta cgattggtcg ttatcaattg gttgctgaac aagaattaga
      901 caaaaacgat ctttcttatc gcgtcatgca gcaggtcttt gattattatc agttgccact
      961 gttcgaggat gaatatcatt atatctatag tgatacattc gacacgattc accagcaaga
     1021 cgtcatgaaa ctagttgaga atgtgattta taaagtaagt aaagcaatca accattcttt
     1081 ttatcaagat ccacaattgt tgacgaatct gtatgctcat ttatccatgc gattatcgag
     1141 aaagaaaaaa tttgtcaatg aatataatcc ttttaaagaa gatatcaagc agaaataccc
     1201 ggaattattt gatgcgatca agcaagcagt tcgacaagaa ataagtgaac aaaatattct
     1261 gatcaatgat tctttcgtgg catatattgc cttacacttt ttggtttcct acgaaagaga
     1321 agaagatcta cgaagcgtta gagccgtcta catttgttca acagggttgg gtgtgactag
     1381 tctgatcaag caaaagatca atgaagaatt aaataatatc actatcgtgg catttgcttc
     1441 agtattgaat gcgcaagaaa tgattgctac caaagatcct gatttggtga ttagtatttt
     1501 tccaattaat ggcatcgatc gtcctttcat caaagtccat ccattaccca ctgaacagga
     1561 cttgaaaaaa attcaacaaa taataaaaaa aatacttgct tcttccaatc agcaggaaag
     1621 gcataacttg aatcttagag aaacgtccat caataaagat actttagaag actttagcaa
     1681 ggaattgatt ttgaaagctt atacgattta tgaacgttta cttgtcattt tggaagcaga
     1741 attgatccaa gaatatcgag aagctttttt gcttcatgta ttcttaatgg ttcatcgtat
     1801 cacatttgat caacaatata tggcagaagg tcgtcaagta gaccagatta ttttagacaa
     1861 aggaacactg gtaggaaaaa tcgaagcagt ttttgcagaa aataatttag gaatcaatca
     1921 gtcagaaatc agtgcattat tctcatatat aaaagaagga gcgtcaagtt agtgaaaatg
     1981 tcagacgagg ctttaaaaat tatcgaggaa agtcaaaatc gggaaaagtt aaaacaagtg
     2041 attgatctaa ttgaagagcg attagtagaa catgctattg taccaaccga gttgcagtgg
     2101 accattctaa ttaatcactt gaatgagatg ctgaaacgtt ctcaaagaaa ggaaacgatt
     2161 cccgtagtcg atcgggattt gttcaaagaa gtatccaaag aagccataac tatttctgat
     2221 gaaatagtta gaagtatcgg taatttaacc gatgatgaaa tatatgtttt gtcgattcat
     2281 tttgaaacag caaaaaataa tta
//
LOCUS       DAYYAG010000221         2266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000221 DAYYAG010000000
VERSION     DAYYAG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.227"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..758)
                     /locus_tag="ACHARQ_002906"
     CDS             complement(<1..758)
                     /locus_tag="ACHARQ_002906"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM8411278.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKM"
     gene            complement(730..1626)
                     /locus_tag="ACHARQ_002907"
     CDS             complement(730..1626)
                     /locus_tag="ACHARQ_002907"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8411279.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1616..>2266)
                     /locus_tag="ACHARQ_002908"
     CDS             complement(1616..>2266)
                     /locus_tag="ACHARQ_002908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411280.1"
                     /translation="FIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYL
                     GTINNIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVI
                     YDNAEKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYN
                     VGINYNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          779 a          384 c          273 g          830 t
ORIGIN      
        1 ttcatttttg ataatttatg aactaaaaaa agatttgctg aaaaaaagag agcaaacatc
       61 actacgtgag aaatcgatgt actgtttcta tcaaaatatg catcatactt cgtatttata
      121 aatattgaac tgatataact ttgtccaata gagaatagga gcaagctaag gattgtaaca
      181 tatccagtaa tctttttatt aatttttaca ataaaaaaag ggagataaaa atatgcaaga
      241 ttatggaaca gtccagccac taagataaaa aaaatatact taaatatagc tttgctgcct
      301 cgcaatttag ttagatgata aaaagctagt aatatgaata tcaatgcgct aaacatacgt
      361 acttgtattg cttgtgaagt aaaaccaaaa ataaaaaaca agaacaaaac taaaaaaaca
      421 ttagttgtga ataatctgat gacatacaat aaagctgaga gtaagacagt agaaataatc
      481 aataaaaaaa tctgatactg actggaaata aaggtacata cgtacataac agctctgaat
      541 cccggttcaa tactatctga actaaagtcc ataccaaatt gtgctatccc attatacaag
      601 acagaatatg cataataatc agcgttttgt gtgttaaatg ccatgatgat ccacattatc
      661 aaaagtagca ccccagacaa aatcttgttc gatgtactcc attttttttg tgaaacactc
      721 ttttcttgat taatctccaa tgacgcctct gtatccatct cgaacccccc ttatagccgc
      781 tctaaatcta ctgagcttat cagtctcaaa agctattgca ataaaaaatt tcaataatct
      841 tttattcata aggcgggaaa attccttatc acttttatac attcttttca ccaatgttga
      901 atttcttgca ataaaatact ttcgtgttga attatgattg tctattttaa ttttttttga
      961 tcctattttt aaattttctc catctccaac gtgatgataa aaatagactt gatttgttac
     1021 ataaatgtta tagccattat ttgcaaccct tttacaaaat tcaacatcaa tctgatcaat
     1081 aaaaaattca tccttcatat ttccaacatc gtcaattaat tcacttttga aaaacattcc
     1141 agaggaattt gcaaatgcta ttttcatgga accactagtt gaagcaaagt gtttttttac
     1201 gtatctccct ctctcatttt tgcaggtaaa agagacagat tcatcattgc tattctcaaa
     1261 gtaattggga acaataatcc caatgtcctt atctttcgct tcaatattgg taaactcttc
     1321 taataatttt aaagaaagat tcttttgtat gatggtatct tgatctagta aaaggatata
     1381 atcacatttc tcttttttgg caatttcaat acctatgttt tgagctgtcg caagcccaat
     1441 gttctcattc atttttttta aaattaagtt ttccatcatt tcaaattgaa aagagttaat
     1501 attatctgag ccgttatcca cgataataag tttgttctgt ttctcttgta ttaacgtatc
     1561 cactaataac tttatgttat tatctggatt gtaggtaaca ataattatcc caattttatt
     1621 tttcatcgtt catcaccttg tacacttcat tccatttcat tttcaatact tgctcagaga
     1681 atatagtggc gtaaattttt ctaacatttt catttatgtt tttattacac tgataaaatt
     1741 tcaacatctc attttcatca acggtactat tataattgat tccgacatta tatcttttaa
     1801 ctatttcttt cgtatctcct gataaatttg aaattatagg taatccaaac ctaaaatact
     1861 caatcgattt cagacttaaa ccaattatgg tatttgactt aaataaattt agtccaaaat
     1921 gacatttatc gagtataact tttttttctg cattgtcata tataacaccg cagtcaacaa
     1981 attcaatatg atttttttca caaatatcag ctaatttagt tttgctttca ccttttccaa
     2041 taaagtaaag atttattttg tttccaaatt ttcttttgca tgttttcaat aattcattaa
     2101 tcgcttctaa atctacgata ttattaatcg tacctaaata agcaaggttc aacgtagact
     2161 ctaagctttt attgactatt ttctctccat aatcattttg gtcatctaca ttgaaagaac
     2221 atggataaat ggtcatattt tttgattctc cggaaatttt tatgaa
//
LOCUS       DAYYAG010000222         2248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000222 DAYYAG010000000
VERSION     DAYYAG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.228"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACHARQ_002909"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACHARQ_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACHARQ_002910"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACHARQ_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACHARQ_002911"
     CDS             1898..2059
                     /locus_tag="ACHARQ_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411281.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          807 a          346 c          378 g          717 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgct
//
LOCUS       DAYYAG010000223         2213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000223 DAYYAG010000000
VERSION     DAYYAG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.229"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..469
                     /locus_tag="ACHARQ_002912"
     CDS             <1..469
                     /locus_tag="ACHARQ_002912"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8411282.1"
                     /translation="FLILILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNT
                     NEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSP
                     IFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            597..1280
                     /locus_tag="ACHARQ_002913"
     CDS             597..1280
                     /locus_tag="ACHARQ_002913"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8411283.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1589..1662
                     /locus_tag="ACHARQ_002914"
     tRNA            1589..1662
                     /locus_tag="ACHARQ_002914"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1623..1625,aa:Arg,seq:tct)
     gene            1821..2180
                     /locus_tag="ACHARQ_002915"
     CDS             1821..2180
                     /locus_tag="ACHARQ_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8411284.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          736 a          345 c          450 g          682 t
ORIGIN      
        1 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
       61 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      121 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      181 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      241 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      301 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      361 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      421 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      481 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      541 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      601 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      661 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      721 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
      781 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
      841 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
      901 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
      961 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1021 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1081 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1141 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1201 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1261 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1321 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1381 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1441 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1501 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1561 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1621 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1681 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1741 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     1801 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     1861 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     1921 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     1981 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2041 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2101 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2161 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaa
//
LOCUS       DAYYAG010000224         2160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000224 DAYYAG010000000
VERSION     DAYYAG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.230"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHARQ_002916"
     CDS             complement(<1..426)
                     /locus_tag="ACHARQ_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8411285.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHARQ_002917"
     CDS             complement(535..1566)
                     /locus_tag="ACHARQ_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8411286.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          680 a          343 c          366 g          771 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggattgg ataaattaaa ccatcacaac gagtggaaat tatatgaaat ttttcataaa
     2101 aattcatata gaaacaaatg acattttatt agaatatgac tttcattaac ataaatgttt
//
LOCUS       DAYYAG010000225         2159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000225 DAYYAG010000000
VERSION     DAYYAG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.231"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..>2159
                     /gene="acm"
                     /locus_tag="ACHARQ_002918"
     CDS             54..>2159
                     /gene="acm"
                     /locus_tag="ACHARQ_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8411287.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVII"
BASE COUNT          851 a          343 c          421 g          544 t
ORIGIN      
        1 gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa
       61 agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca tcactgattc
      121 ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact tcgttgaccg
      181 tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg tttgacgaaa
      241 aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt gaaggaaata
      301 ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa aatgttggaa
      361 ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag
      421 actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc gataccggtg
      481 aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact gtaaatgtga
      541 caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga gacatgctac
      601 ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc acttatgttg
      661 aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat ccaagtacgt
      721 ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa gagggaatcc
      781 aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact gataattaca
      841 ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg agttataaga
      901 cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct tggtttaaag
      961 aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa aatatttcag
     1021 cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa tatatcaatg
     1081 acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat gaacagccta
     1141 ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct agtatcaaag
     1201 gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg attgattttg
     1261 atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca gagggagtca
     1321 gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa atttggaacg
     1381 gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt acaaacaatt
     1441 gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact tcagtgactt
     1501 gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag
     1561 tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa aacgggctag
     1621 tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca tgggaagaca
     1681 atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta gccgacggtc
     1741 aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc
     1801 tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac aacgtgcccg
     1861 aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa
     1921 atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag aagacagaga
     1981 gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat acaggagaat
     2041 ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag ataacaaata
     2101 aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct gtaatcatt
//
LOCUS       DAYYAG010000226         2130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000226 DAYYAG010000000
VERSION     DAYYAG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.232"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1019)
                     /gene="cysK"
                     /locus_tag="ACHARQ_002919"
     CDS             complement(93..1019)
                     /gene="cysK"
                     /locus_tag="ACHARQ_002919"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8411288.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1159..2046)
                     /locus_tag="ACHARQ_002920"
     CDS             complement(1159..2046)
                     /locus_tag="ACHARQ_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8411289.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          589 a          475 c          356 g          710 t
ORIGIN      
        1 ttcaaataat ttttctcatc ataaattttt gtgtgttctt ttcatcttta aaagtttttt
       61 acataaacct atctggagaa aaaccaagtg ccttatatct cttggtaaag ggctgtagat
      121 aaataacgtt caccattgtc gggaaccaca gctaatacct tcttgccttt tcctaattct
      181 tttgcgactt ttagcgcggc agcaattgca gcgccagatg agatgcctac taaaatccct
      241 tcttttcgcc caacttctct agctgtttcc atcgcttcgt ctccactaat agaaaggact
      301 ttatcataca cagaagtatc cagtgtttta ggaacaaaac ctgtaccgat tccttggatt
      361 ttgtgaggtc ccgcttcttt cccttctaaa atagcagatt ctgctggttc taccccgatt
      421 aattcgattt tcggatacac acgttttaat tctcggccag cgccagtgat cgttcctcct
      481 gtaccgatac ctgcaacaaa cgcgtccaag ccatttactc caaatgcttg atgtatctca
      541 ggaccagtcg tcttctcatg cacaagagga tttgcttcat tttcaaattg taatggtaaa
      601 aagtagccgt tttcttttgc caaacgttct gcttctctga ttgatccaga aataccatcc
      661 gcaccaggag tcaaaatcaa ctctgcgccg taccctttca tcaataaacg tctttcgata
      721 ctcatcgtct caggcatcac gatgattact ttatatcctt ttgcaactcc taccatcgac
      781 aatccgattc ctgtatttcc agaagtaggt tcaatgatag tatcacctgg tttcagtaga
      841 ccatcgtgtt ctgctttttc tatcatactt aaagcaatac gatcttttac acttcctcct
      901 ggattaaaaa actcaagctt tacaaaaaca tctgctgaat cttctggtac gattttattt
      961 aatttgacga ttggcgtttt gccaatcaat tcggtgactg agttatagat agctgtcatc
     1021 ttcaattcct ccttgtcttt gctcttctta cttatcataa tacaatctgt tcatttatgg
     1081 cgagtgattc gtctttgact agcaaaaaaa agacaaagat ataccatata tggtaatttc
     1141 tttgcctttt tatctaaatc aaacgtttag ttcttgacca actaataaag aataactgga
     1201 catagacatg ccatttttcg ccattagatc atcaacagac atgccaaatt tttgtgcaat
     1261 atcccatagc gtatcacctg acacgactgt atatgtctga ccattatttg tatttacatc
     1321 tgttgttgaa gatgatgacg ttgaagtcgt cgtagtagtc gttgttgttg tacttgtggt
     1381 tgttgtagcc gaacctgttg atggcgtatc aaaacgggtt aaattataag tattaatcaa
     1441 tgagttcaat ttggccccat aatttggatc ggtagcataa cgacctgtca agtacgctgt
     1501 tgcatcataa aaacttgttg tattgctttt ccacacgcca gcataatgat aatcgcccgt
     1561 ggaaaaactg gttgaacgaa gaacattcgc atggtcttgg aaggattccc aataagaaga
     1621 atattttcgg aatggctcat tcatcgttac ccattgtccg tttacatatt ctttggttgc
     1681 catataaacg acctgtccgt tatagcttcc tttcacaccg aatagattat aatttggcgc
     1741 actagctaat ccggaagtgc catatgcact ttccaacaac gcttgagcga tcatgactga
     1801 agcatacaag tcattacttg cagcaacttc tgacgcagac cagccgattt gatcgataaa
     1861 cgcttgagga tcagcgactg tttgagatcc ttgttcatct gcatgaacat gttgcggtac
     1921 cgctgtaacc atcatcggcc caactaaaag actcgttcca attaaaggag caatcgcttt
     1981 tttattgatc accatttttc ttttaggtct atggtgccgg gcactgcggg tcttcagctc
     2041 ttgcattcaa attcctcctc aaaataaaaa aacttaataa aaagttctct tcttaaaaca
     2101 aacttaatta attaaattat acttttaatt
//
LOCUS       DAYYAG010000227         2105 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000227 DAYYAG010000000
VERSION     DAYYAG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.233"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..357)
                     /locus_tag="ACHARQ_002921"
     CDS             complement(127..357)
                     /locus_tag="ACHARQ_002921"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8411290.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(451..789)
                     /locus_tag="ACHARQ_002922"
     CDS             complement(451..789)
                     /locus_tag="ACHARQ_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411291.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1717..1833)
                     /locus_tag="ACHARQ_002923"
     CDS             complement(1717..1833)
                     /locus_tag="ACHARQ_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8411292.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          687 a          376 c          281 g          761 t
ORIGIN      
        1 acaaaagtta aaataaaata ctctggatac tctttatact gcattaaagt tagtaatatt
       61 aacagcaaag atatcgaaaa aacaataaca taatatgtgt caaaattttt cataataaga
      121 actcccttac tgttgcatac taacataatc caaaaactct tctacttcag ctaaagacat
      181 tccactttct ttagtccaaa aaacagtttt ttctatcgat ccttctctgt aaactgtaaa
      241 tgtcggagtc gagttaattg agtagcgact cttgaattct ttccactcat tttcatcttc
      301 tctaactttt gctacattca cgtaataaat attttctttt cccttttcta ccagcatatc
      361 gaatagcttc ctgcttttga tgatcgattt cttgtgctac atctagtaaa ttttcatcaa
      421 tttctatatc ataaagtatt tcactagtgt ctacatccac aatatatact ttttatcagt
      481 agcaattttt gagtaatccg ttaaaatgat ctcagtctcg tcactttcat cagtataaat
      541 atacgttaac aatgaactca ccttttcaat cggtgttagc tctaagccta ctgtattttt
      601 tactattgag tcattattta ccaccataac ttcgtactgt atataccatc ctatttggtt
      661 tccatcaata tcagtaattg gctcactagc taatggagtc tccggaacaa tattttcctg
      721 atatttctta aactcttctg tagcacgaaa aaggttatca tgttcctcac ttgaaactgg
      781 ttgagtcata gaaaatgtta cttggctaaa cagagggaaa ataaacacta caatcaacaa
      841 agtataaaaa aaagcccttc tttttcattt tttatcttcc aatcacaaat tgaaaaactt
      901 attcctgcaa ctttgagtct agtatcacaa tcgactgact acaactattc atttacattc
      961 tactttaagt tcctccttaa ccattaattt tattaaagat acttgtaata gtgattctca
     1021 caaaatttca tgaaaaatac tttattactg tatctaaaat tccaatacac aacttatcga
     1081 ctattatctg aaaaccaaaa cttattattc cttgatgtta ttctagcaat agtaactgct
     1141 tttttatttt tttgcatatt ttttcggcgt tactttttaa agttaacttt ttcgacaaat
     1201 agaaagagat acgaatcact gatttttttg aagaaaagaa atggagtgag ctgaacgtta
     1261 gagatattgg gagctttcgt aactgcttat taaacgataa tggttatttg caaccgtatt
     1321 gctcgcttct acatgaaata tttcatgaaa catttcgcaa aacctttgat tatgtagtta
     1381 cgtttccatt cttgactaaa aatgtttcaa aaacgaacaa aggtgttaaa aggtaaagtg
     1441 atcaatacta ctggtcaaaa taagaggttg aaagggaatt gaaaaataac tacagataat
     1501 acttttataa acgtacgtgc tttgtggttc tgtagttaac tatataactg gaattaggga
     1561 atggtatcaa tttgaaaaga aagattctac caatttatca tatgttaaaa atggaaaatc
     1621 aacagcagtt ctttcggaaa ccttacacaa aaaccgatta ttgaaaatag ccgaaaaacg
     1681 tttttcggct atttttttaa ttaagtttcc ttcttcttac cttttcccac tattccgttt
     1741 cacatctttg atgatcttca gcgcacgttt tcgtgttttg atatttgggc ttttcaagcg
     1801 gcggtatgca cttgagatat ctgttttttc catatggacg acccactttc ttctttgatt
     1861 gtagtcatgc taaaaaacac aaatcgaaac aattacgatt tgtgttttaa atggtatcaa
     1921 aagttaaaat gaaagtcaac ttgttaaaac agtcgatcag actagctgat tgactcctat
     1981 ctctggaaat aaattttcat tattacgttt tagctaccta aaaaaggccc tcttgtccgt
     2041 taactttaat atctattgtt atagctagtt tacctattga acgtactgtg tgttcgatag
     2101 aacac
//
LOCUS       DAYYAG010000228         2067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000228 DAYYAG010000000
VERSION     DAYYAG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.234"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..418)
                     /locus_tag="ACHARQ_002924"
     CDS             complement(131..418)
                     /locus_tag="ACHARQ_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8411293.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(589..1941)
                     /locus_tag="ACHARQ_002925"
     CDS             complement(589..1941)
                     /locus_tag="ACHARQ_002925"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8411294.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          565 a          412 c          357 g          733 t
ORIGIN      
        1 aaaaaaaggg gttgacaaag cttgtcataa cccctttttc tgattaaaag atagtaaaga
       61 gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc ggccttttca
      121 aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct tcatatgtgt
      181 actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg aaaggaaaac
      241 gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc tcttgcgttt
      301 cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt aatttcttcg
      361 aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct ttattcatct
      421 tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat gtaacagctt
      481 tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg tacttttcac
      541 aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt attgtgcagt
      601 ttcgatttcg cctagcgctg cttgtgcaag taggttcaag taattccatg ggcggtcaaa
      661 atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt tattttgaac
      721 agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga actgagcacc
      781 tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt cagtagttgg
      841 catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta gatcgttcaa
      901 agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata ggtaaagtcc
      961 tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat ttcccacaag
     1021 caaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc cacttggatt
     1081 gtagttgact actgcagagt ctccagctgc aaaaatgtct ggattacttg tctgcatgta
     1141 ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga tcagttttgt
     1201 atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat ctgttggtgt
     1261 agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat tttcgcctag
     1321 cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga atggtttatc
     1381 tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt ttccagattc
     1441 gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta ctacacgttt
     1501 tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc ataggagtac
     1561 atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag ttgtcatcac
     1621 taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg ccgttactgt
     1681 tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg ctcctaatga
     1741 agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta ctccgccgac
     1801 ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat atactgtcac
     1861 ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg catgagtaca
     1921 gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc ttcgacaaaa
     1981 ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc acaaaggttt
     2041 gtttgtagtt tcattccttt tcaagca
//
LOCUS       DAYYAG010000229         1950 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000229 DAYYAG010000000
VERSION     DAYYAG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.235"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..439
                     /locus_tag="ACHARQ_002926"
     CDS             <1..439
                     /locus_tag="ACHARQ_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411295.1"
                     /translation="LMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGN
                     DHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDA
                     VLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(500..865)
                     /locus_tag="ACHARQ_002927"
     CDS             complement(500..865)
                     /locus_tag="ACHARQ_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8411296.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(941..1186)
                     /locus_tag="ACHARQ_002928"
     CDS             complement(941..1186)
                     /locus_tag="ACHARQ_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8411297.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1256..1510)
                     /locus_tag="ACHARQ_002929"
     CDS             complement(1256..1510)
                     /locus_tag="ACHARQ_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411298.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1674..>1950)
                     /locus_tag="ACHARQ_002930"
     CDS             complement(1674..>1950)
                     /locus_tag="ACHARQ_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411299.1"
                     /translation="VAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLVMMLSYKGKRY
                     ARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          713 a          343 c          349 g          545 t
ORIGIN      
        1 ccttatgata aaaaaactat ttttactatc aatattaacg ctagcaatgg caggttgttc
       61 tgactctggc ttagcaactg aactgatcac aaaatcaact gaagccacga cagaggagtt
      121 aaaatgtgga aacgatcaca gtgactacag aggcttaaat cgacgatgca tgggaatatt
      181 aggcagaagc aattgtaagc atcgatgtag taatcgaaga aggtgtagaa gaagcaacag
      241 aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt gcatcgtata cagacgtcga
      301 agatgcggta cttttaaacc acgtcaggag atgttcattt atgtctaggg aagttggcga
      361 tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag gatgaagata accctaaaac
      421 ttacgacatt ttagcttgat ctaagatggt taaagctttt taatgaagta agaggagata
      481 gaaggctttt ctagttatat taggtaagat aaattccttt ttgctccaaa tttgctaaac
      541 attctgctag ataaacttca tcgtgttccg gataaatcgg cacgttgcgc tctacagtag
      601 gtaaatcaaa aaatgcttcg catgatttgc cacgatacat aggatttttc cactctggtg
      661 ctgggctgta accacgtttt tccatttcat ccatgacaag ctgatgatat tggaataatc
      721 tataaggaga atagtgaaaa acataatcca cagtagcatg tttctttccc cagccatttc
      781 ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg ttgacgagga agctttgaaa
      841 tcaatttttc atgccataat cgcatagaaa tgtctccttt atatatagat tgttaacctt
      901 agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa tcacttcagt ttgcttgctg
      961 tttctgagcg accaattagg taatcaacgg aaatatcgta aaagtcagcg atatagatta
     1021 attcaatgat gcttggaatt tcttctcctt cttcgtactt acgatatttg aataaaggaa
     1081 tacttaacgc gctagaaaca gctacttgag ataaagatcg gccagttctt aagaaattca
     1141 gacgacaacg taaagtatcc agtaaaatag cttcttcgat actcatttaa tcacctttca
     1201 ttatagtaga atagttataa tgactataga aagctacaca tgcaatcaat agtactcaat
     1261 gttttttatt taaacgtatc aaataaagaa tgacgatcaa taaagacaaa accagtcctg
     1321 ataattcttc tataaaaaca ctatcgggtt caaagtagtt gtaattaaaa actttccaca
     1381 taaagtagat agccggaaga gtcaaaatag ctaaataaaa ccgttctttg ttccaattca
     1441 taagataaac aatacttgcg accagtgcga ccgcaggcac ccaaaacaaa actctcacca
     1501 acgtaatcat gtaacggact cctcaacgat tcatataata aaagataaca tgttattgca
     1561 tacatcacaa atgaaatgct aggaaaataa aagaagaata gatcaatgaa aaatgaaaaa
     1621 gcagaacagt tttctgctga taagcaggaa ctgttccgct gaatgtgtct tatctatcga
     1681 gaaaaaatca atggattgat atctaagtaa ttcaatacat ataaaaagag gacgatcgca
     1741 ttcactaaaa aggaaagaat gatgatccca tagcgaatgg aaacatattt aacaaatact
     1801 tttgtttgaa tacgggcata tctttttcct ttatagctga gcatcatgac aagaagcaaa
     1861 agggtaatca caccttgaaa aatcattcca ataaacagaa ttcctgaggt ttgataattt
     1921 aattggagaa aataagctgc tgccgcaact
//
LOCUS       DAYYAG010000230         1944 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000230 DAYYAG010000000
VERSION     DAYYAG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.236"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHARQ_002931"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHARQ_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8411300.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHARQ_002932"
     CDS             852..1040
                     /locus_tag="ACHARQ_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8411301.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHARQ_002933"
     CDS             1053..1610
                     /locus_tag="ACHARQ_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8411302.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHARQ_002934"
     CDS             1642..1884
                     /locus_tag="ACHARQ_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8411303.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          301 c          400 g          528 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cact
//
LOCUS       DAYYAG010000231         1904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000231 DAYYAG010000000
VERSION     DAYYAG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.237"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(680..753)
                     /locus_tag="ACHARQ_002935"
     tRNA            complement(680..753)
                     /locus_tag="ACHARQ_002935"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:Ile,seq:cat)
     gene            complement(942..>1904)
                     /locus_tag="ACHARQ_002936"
     CDS             complement(942..>1904)
                     /locus_tag="ACHARQ_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8411304.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          570 a          361 c          337 g          636 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatga ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga
      121 ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta ggctacctaa
      181 aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt ttttaagtta
      241 gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc tataaaatca
      301 aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca ctttttttca
      361 aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg taacacactt
      421 atggggaagt ggtttggggt gcgctttaaa ctcttcttta ttattatctc acaatttaac
      481 ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag aagtataact
      541 atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt
      601 ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaaattcta acacagaacg
      661 attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg acggttatga
      721 gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa agccataaac
      781 aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga gtgtgactat
      841 tatgggtgat agtgaagata tgcgataaca tcactatttt atcgaatgat ttttatagtt
      901 gtcaatatag ttatgtaatg ctcctcaacg aggagctatt tttatcgttt aggaatattt
      961 aaataccaac gtttgtcatg gaaatcttgc gcaccgcctt tagtgtttcc ctctggatca
     1021 ttcgttgccc gcatcatgac atagactttc ttattaggga agttacgcat gttaaaagat
     1081 acatgatagc caacatttcc agaagtatta taagcttgat ttacatctgc tctataaatt
     1141 ccatcagctc ttactcgagc taattctttc ccagtattgt aatccataat gaagatatac
     1201 tcgtatttat agttagcaat gtgccatcca gctacatgca aatttgcatt ttcgatttct
     1261 ccaaactgat caatgtgggc gtaattcgtt ccatctgtca gtgtaggatt agctgcacca
     1321 gctcgtgttg gatcaatgac aggcttgttt tcagaagttg ttggattttc atcggtaaat
     1381 ccatgagcta aatcatatgc taatttttct ttacttacgc ccatttcaga aagataaccg
     1441 taaggatctg tatgatcgcc ccagatgttt tgtgttaccc ataaatgcga tttgattccc
     1501 ggttggttat aaggagtgtc caacgttaat ggaataccat atttcattgc tgaatctcta
     1561 gccaattcaa cgtatgcctt gtagtttttc tcaaacgttg ctttatcatg tgtgtgttgt
     1621 aactcaatct gcacaggact gttggcatta gcatacgaac cagcaccgta ctgtacataa
     1681 ccaggttgac cgacttgata aacaattccg ccgtctccca caatataagc agtataagcg
     1741 ctagtccatg aacgttgcat atactgcgct tcattgcgtc ctgttgctgt ttcattagcc
     1801 gtttcatgca gtaaaatata ctgattattt gctacttgtg agctaccttc atttgcgccc
     1861 aaattaaatt cattgttgat agtataggca aatccgttaa ttgg
//
LOCUS       DAYYAG010000232         1887 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000232 DAYYAG010000000
VERSION     DAYYAG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.238"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            500..832
                     /gene="traE"
                     /locus_tag="ACHARQ_002937"
     CDS             500..832
                     /gene="traE"
                     /locus_tag="ACHARQ_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8411305.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1451..1792
                     /locus_tag="ACHARQ_002938"
     CDS             1451..1792
                     /locus_tag="ACHARQ_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8411306.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          651 a          254 c          409 g          573 t
ORIGIN      
        1 ttcgcttaca ttttttgtat aaatttaatt gtctgctatg cttgtgatga attgattttc
       61 gttttgcact tttggatgat aaatttggac aattgaaaat acaaaaaaaa gaaaggcggt
      121 atgaactcgg aaggctggta accttcgtca gcgttcatac acggcattaa gatttggccc
      181 ttaatgcgtt aacctttcag tgttacggta ttacccgcaa tggatttagt ataacagata
      241 ttcaaaagtg ataccacttt ttttggctgt gttaaggtta aaaatacgtg atattacgat
      301 gaatctatgc ctaagcataa tgttataaaa atgtccgtgt ggctctttgc cgtatgacga
      361 acgctgaaat tagagggaac gcatgtttgg atgcgttccc tcttttttgt ttgtcatgaa
      421 cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa cttgttaaaa atatttaaag
      481 aaattgggtg gtttatttta tgtcatatac aggaattttg agtctcaaag atatttgtca
      541 ctacggtaaa cggtgtaccg ctacagagaa aatcacgaag aaactatcaa ctggccaaag
      601 taaaaccgtt gtgcagtgta agaaatatat aatccagaaa gacaaagtct cagaggaaat
      661 gatttattac attgggaaac aaaagcaaat aattttaaaa gatcctatcc ccttaaaaga
      721 gctatatcca acaatcaaac atgtttatga ccaaaacggt gtactgattg gtcgaagaaa
      781 aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt ttgatcggct gaattgatca
      841 ttctcataac ttgtatttta tcgtttttcg cttgcttgaa aatattgtcg agacttctaa
      901 aggaacttag agtcactaga aaaataagga gtagacttga tctatcaaca attcccttcg
      961 gggagtgacg gggtaggttt taacgattgg taacgtaaaa aattggtcaa tcgagtaaat
     1021 gcgcttgttt ggatagtggt gaaatgaatt tgtgtaaata ataagcgcag agtaaacaag
     1081 agggaattta gaagttagac aagttagaat agcgtctaga ttaatcatga gcagctacta
     1141 agggatctag actttgacgc aaactcatgg aataaggagt aggtcttctc gtaatgggga
     1201 ggacgcacaa gaaattgtgt cgtgtgctgc agtacatgta gatacttgcg aggacgttga
     1261 ttattggatc aatcgagcat ttaccgctgt ctactagggt ttctcgtttc ggacgccata
     1321 aacgagtcga aattttttat gtatttttaa gtaaacgaaa aacgataaaa tatgaacaag
     1381 tcctgaataa agtgaaaaaa ttgatataaa acatagagaa aatgaaattt acgtaatgga
     1441 ggaatgacaa atgaaaaatt atgatccgaa tatacgattg ggcacacata ctattaaggt
     1501 ttctttccaa cgttgggatt acaaaggatt tgtgacgttt agaaggggcg gaaattgtaa
     1561 aggattagat gttttagcat tagacgagga tgatttatac gatcaaacat tgactgataa
     1621 tccaattgga ttcggcttgc tacctgaaga tgatgaaggt aatgaatggt ttaaaatgac
     1681 gcttatgaat gataatggag acgaattatc tgttgaagat acttggtcat atttgagtga
     1741 ttatattgtc agctttgaaa tcattgaatt tgtagcggat aaagaagaat aaaaactgga
     1801 ggaataaatt ttacagataa aggcttagaa ggagtcacga cgattaataa aaaggtagta
     1861 ttaccttagg cattgagtaa ggaattt
//
LOCUS       DAYYAG010000233         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000233 DAYYAG010000000
VERSION     DAYYAG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.239"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHARQ_002939"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHARQ_002939"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8411307.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYAG010000234         1858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000234 DAYYAG010000000
VERSION     DAYYAG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.240"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..830
                     /locus_tag="ACHARQ_002940"
     CDS             <1..830
                     /locus_tag="ACHARQ_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8411308.1"
                     /translation="SLTDYLYEHREQETILRELKKEFSQNKFSHFLDLLIDAGLIKRE
                     ERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSVDEQKLAMGEVIWAYCFEDERKE
                     AYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFPLTLANYFFVQKNQLPVTKAFKE
                     LAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSIFHQSLLVTNTIKEEESFTLELP
                     IVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSKKFIPHAFSYIKEYGTVLVSKT"
     gene            964..1089
                     /locus_tag="ACHARQ_002941"
     CDS             964..1089
                     /locus_tag="ACHARQ_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8411309.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1223..1855)
                     /locus_tag="ACHARQ_002942"
     CDS             complement(1223..1855)
                     /locus_tag="ACHARQ_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411310.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          710 a          279 c          353 g          516 t
ORIGIN      
        1 cttcattgac cgattatcta tatgaacatc gagaacaaga aacgattcta agagaattga
       61 aaaaagaatt ttcgcaaaat aaattttccc attttcttga tttattgatc gatgcaggat
      121 taatcaagag ggaagaacga agatatcatt tgaactttcc aatatttgat cctaaagatt
      181 acttgcaaca agccacttcg gctgctgaaa ccatagctga acaattgaag agactctctg
      241 ttgatgaaca aaaattagcg atgggagaag ttatttgggc gtattgtttc gaagatgaac
      301 ggaaagaagc gtatttttat ggagtaagaa acagccgaga gacagaattg ctgaggacaa
      361 ctgcaggaaa tgagaagtac cgttttatca cactttcctc tatagaacac ttcccgctta
      421 ctttggctaa ttactttttt gtacagaaga atcagcttcc tgtaacgaag gcattcaaag
      481 aattagccga acttattgga gacgtaaatg aatcgtactt ttttgatcaa atagaagtta
      541 tcgttgacag gatacgtaag aataaatata aaaatcgccg gcctagtatt ttccatcagt
      601 ctctacttgt gacgaatact ataaaggaag aagaatcatt cacattagaa ttacctattg
      661 tcgaaaaaaa caattttgag attgaattac caacattgga tccttcattg acaatggagg
      721 aaatggcctt tcttaaacga caaatctttt ctgagctgag caagaaattt ataccgcatg
      781 cattttctta tatcaaagaa tatggaacag ttttagtgag taaaacctaa gagagcagta
      841 agcaaaaatt atactgactc ttgcaggagg agcctttctc ttcctttatg gttaaaagaa
      901 ttatttgagt attttgccta tctaccacct attagattca ctacatggtc tggaaacaaa
      961 ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg aagtagtgat aagaaaagtt
     1021 agatttttaa ataatcaata ttatgattca gtcaagtatg gcattttatg ggaagaacta
     1081 gctgattaga ttaggaagtc catttgataa tccagaatca ctaagaaacg aatggcgagt
     1141 cattaataat ttatccaatc ttgggttatt cttttagatt gaagtgttta aaactaaaaa
     1201 agcatttatg aagtcgtaat agtcaggctt caaaatgctt tttattttgt ttgttttttc
     1261 tttgccacat aaatacacct agactgacga tgaagagcat atccaacaga tttcctgcga
     1321 cttggatcgt cgaagggttg gtcagtggca ttaaagcaag gacaaaaaag gaaaacaagc
     1381 tgtaaataaa acaggtgaca ggaactaatc gaaagaagag cggacatttc aattggctaa
     1441 aaatgagtga aaaaataaaa taagtaaaaa tggataaaat cacgactaaa ccattcatga
     1501 taaagaagaa aagcgtattg tgattaaacg aataaaaaag aattgctaaa aaagaaaacg
     1561 ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt accaggatgt cgatgaaaaa
     1621 aataggcacc gaaataaaca gaaaacagta agccgcaaag agaagacaaa atcaaaaaaa
     1681 acggattttc ttcggcttct gtgatatcag aatgatccaa aagcaaccag cctaatgcac
     1741 tagaaggcgc atgtgaggtc ttaaggtatg aataacatag aaaaaataag atcgtcaaag
     1801 ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct acgttgaatt tccataat
//
LOCUS       DAYYAG010000235         1844 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000235 DAYYAG010000000
VERSION     DAYYAG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.241"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..463)
                     /locus_tag="ACHARQ_002943"
     CDS             complement(65..463)
                     /locus_tag="ACHARQ_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8411311.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(460..1233)
                     /locus_tag="ACHARQ_002944"
     CDS             complement(460..1233)
                     /locus_tag="ACHARQ_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8411312.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1230..>1844)
                     /locus_tag="ACHARQ_002945"
     CDS             complement(1230..>1844)
                     /locus_tag="ACHARQ_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8411313.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
BASE COUNT          572 a          467 c          288 g          517 t
ORIGIN      
        1 aaaaaagcgg aaataacgct catccgcctt cttgtttgtg tttatttcct cttcaaacgc
       61 ttgtctatga ttcagacttt tctctatcgt tattttcatc taaaagttct tgcattccga
      121 taatttctcg atattgatga atcttagtga taccaatgac tttgtctaca tcaatactaa
      181 aacaaatacc attacctgga atatcgatgt tgatgccttg accaatcgca tccattactt
      241 tattcgtcaa actatcgggt actaatgtca acacaatatc tttttctggc gaaatgtcca
      301 aacctaaaaa tttccctgaa tcatgtacac cagaacctct tccatgtaag atcgttccgc
      361 ctgctgcacc tgcttctttt gaaaagtcga taacatcttc gcccattcct ccgtcaacga
      421 tcgtcacaat catgtgaaga ttcaatgcta caagatcttt catttgcttt cccccaattt
      481 ttgcaatctt tctttttcga ttctgtcttt ttcatctttt cgtttataaa tatatcctag
      541 tactaaagtg gataatattg gtgtcaatgc aatcattgca accacaccaa acccgtcaat
      601 gatcggatct cttccttcaa tttgtgcagc gattgccacg ctcattgata aaataaatgt
      661 cgaacacatc gggcccgttg ctactccacc gttatcaaaa gccattgcta aaaagatctt
      721 gtccactttt cgtcctaaaa taaatgctag cccgtatcct gggatcaaga aataataaag
      781 agagaatccg acgagcaaac gccacataga caatcctact gctgcgccga tcccaattga
      841 aattaccatc agcatgattt tcgatcggat tctcccctcg ctcaattctt caacctgttt
      901 gatcattaca tgaatcgccg gctcagcaaa ccctactaag aaccccatta aaaagccaag
      961 gggaaccagt atataattgt aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac
     1021 cggtacataa gccatattga ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa
     1081 gaaacctttc ataatgtcaa agaaacgctt tttgcgcata cgaaaactaa tgatattcat
     1141 taaaacaaat aggattacga taggtgccac tgccaaaatg acttccatgg taacgatttc
     1201 gattccatct atcacttgct gccagaaact cattggaaga tcacccccaa aatcatcacg
     1261 gcaagaatcg gccctaacga agcaataccg accattccaa aactgtcatt ttcatttttc
     1321 ttttgtttga tcatactagt catccctccg gcaagggcca agataaatgg aacagttact
     1381 ggaccagtag tcactccccc cgaatcaaaa gcaatcggca taaattcgga actggtgaaa
     1441 aatgaggcga tcaaaacgcc tatgtagcct atagccatca attgataata ggatagcttg
     1501 aatacgaccc gaagcagcgc aaaggctaga aatactccag tgccgacgct gacgatccca
     1561 atcagcaaca ctctgccaat ctctccgtca gatatatcat atacttgttg tcccaaaact
     1621 tgcacggaag gttcagcaat cgtgatcacg atcccgatag caaatcccaa actgaccaaa
     1681 acggtcaaac ttttcttttt gatcatatat ttccctacta gatctccgac ttccatcata
     1741 gaataatctg ctccaaacaa aaagagcgtc attccgatca tcatgattgc cgcaccaacc
     1801 agaaatgaag cgaggtcttc tccttctaac ggtgcaaaaa caaa
//
LOCUS       DAYYAG010000236         1785 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000236 DAYYAG010000000
VERSION     DAYYAG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.242"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1676
                     /locus_tag="ACHARQ_002946"
     CDS             63..1676
                     /locus_tag="ACHARQ_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8411314.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          566 a          320 c          382 g          517 t
ORIGIN      
        1 acaattattt gctgtttttt accataacat agtacgatca acgtagaaaa ggagatgttt
       61 ttatgaaagt tgttgtcatt ggcggtgtag ctggcggtcc ttcatttgcc actcgtttcc
      121 gtcgattgaa tgaagcgcac gaaatcatta tctatgaacg cggagaaaat atttcttatg
      181 caagttgtgc tttgccttat tatttaggag gtgtgatcac tgaccgtgac tcgctgatcg
      241 aacgtacacc agaaatattg aaaacaaaaa acaacatcga tgtctttact aaacacgaag
      301 taacagcaat cgatccttct actaagcgat taacagttaa agacctatcc acaaatgaag
      361 aaataaaaac agattacgat aagttgatca tctcttctgg tgctagacca gattatccgg
      421 atattcccgg agtttttgaa gcagaaaacg gctttgtcct ccgtagtgtg acggatgcgg
      481 atcgaatcaa atcgttcctt gaagaaaaaa atccacaaca tgtcgtcatt cttggtgcag
      541 gtgttatggg tctggaatta gctgagaatt tcaagcatcg cggcttagac gtgactttaa
      601 tcgatcaatt gccacaagtc gctttccctt atgatccaga aattgctaat ttagtttatg
      661 acaaattgct gaaagaagga ttagccgttc atttagaaac aagagttact gagatccgtg
      721 ataaaggtcg agaaatagta ttatcagatg gctccgtcct ttctgctgat atgctaattt
      781 ttgctgttgg tgtttctccg aagaatgaag tggtgaaagc aaccggcata caattatcag
      841 atacaggtca gatcattgtt gatgaccagt tacaaaccaa tctgccggac atctatgcga
      901 ttggcgatat tatcgaaacg actagtgtag tgactggtca gccgatccaa agtatgcttt
      961 ccagtgcggc caatcgtcaa ggacatatgc tggcagatat tttaaatggt acgcctatgc
     1021 gctatcgcgg atatattggt gcaggtgtcg caaaaatctt tgatcataca gcaagttatg
     1081 cgggaatgac agaacatgaa ttaaaagcat caggcataac aaattataaa actgttttta
     1141 tcactccctt tgaccatgcc tatttctatc cagaagctac aagattaaat ctaaagctga
     1201 tttttgatgc agatagcggt cgtattttag gtggacaagc atttggagaa aaaggtgtcg
     1261 ataaacggat gggagaactt tctgtagcaa tcaccggaaa cttgacagtc tttgatttgc
     1321 ctgatttgga gttgccttac tctccaccgt attctactac ccgtgacccg ttgaatatag
     1381 ctggttatgt cgcaatcaat caaatgacga atatcgtaga aacgatcaaa gcaagtgata
     1441 tacccgaaaa cgatttgaaa gaagcgttct ttttagatat acgtgaacct aataaagcac
     1501 cttctggcag tatctcggca acaaaaaata ttccaatgaa tgagttacga gatcgaatca
     1561 atgaaatccc aaaagataaa aaagtttaca ttactttcag aagaggattg aatacttata
     1621 cttctgcccg aatcttggca ggtttgggta tcaaagcgat tttgattgaa gaataaaaaa
     1681 acgaccgcgg tgggtagccg cggtctagga gttattttta cttgaggagt aaaaatgaaa
     1741 aagtatatag ggttgttggt tggtatgtat atagaataga agata
//
LOCUS       DAYYAG010000237         1775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000237 DAYYAG010000000
VERSION     DAYYAG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.243"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..380
                     /locus_tag="ACHARQ_002947"
     CDS             183..380
                     /locus_tag="ACHARQ_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411315.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            486..968
                     /locus_tag="ACHARQ_002948"
     CDS             486..968
                     /locus_tag="ACHARQ_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411316.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1026..1622)
                     /locus_tag="ACHARQ_002949"
     CDS             complement(1026..1622)
                     /locus_tag="ACHARQ_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8411317.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          557 a          270 c          356 g          592 t
ORIGIN      
        1 tttaattttt tgaaaaaaat tcttctaaat acaacagctg ttttaattgt gaaaacgaaa
       61 acagtacgct ataatgcctg taaggttgat ttttaactgt aaataaaaat caatgagtta
      121 aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt aggaggattt
      181 tgatgacgga agaaatgatt aatttaggag aacaatatgc atgcaaacca attggtttta
      241 caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg gtcaaagttg
      301 cccaatgtgc tgcagaggac caagaattat tagatgaaaa agcaagcatg gttgttgcaa
      361 aatatgatac atttgaataa agaaaacaat tggacgtgcg gcgtgctgca agtccaattt
      421 ctttgagaag gtaaatcagt attcttttcc tattttctgg agaagaaaaa gcgaggcgag
      481 ttgtcatggt aacattcttt acggttctat tgtttttatt cgttgttgtg atgtttttat
      541 ttctttggct ggttcgaaaa gaaattattt atcggactgt gcgaaatcga tgggtctact
      601 tggtgatacc cttcttagtg tcactagtca tttggtatac actcatcagt catcctacag
      661 cagatgaatt ggcaaaaggg atcttgtcag caatgatttt tattagtttt ttgttggaca
      721 gtagaggaat cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa ggtgttcctt
      781 tttctgagat caataaaatt gtattatatc agccaaaagg ttctaaaatc gtaaaaatga
      841 attttttccg aaacggatgg cgaggaccga tgctgaaatt ttctgcatca ttagaagaat
      901 tagttccatt tttgtcccaa cgcttaaacg atgaggcaga gattgatatt atgattgatc
      961 cagaataaaa acagaggttg cgacaagatg tttgtcacaa cctctgtttt ttatttcaaa
     1021 taaaattatc caaatacgcg gaagcgatcg gcatcgttca tgtattcttt ttcctcagcc
     1081 ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt tccagtttga agggatattc
     1141 cattcttttg caactgcttc gtcaataact gggttgtaat gttgcaagtt tgcgcccaat
     1201 cctttatcaa ctaatgctac ccaagtatta gctgttgcga ttccgtttga ttgttctgac
     1261 caatctggga agttgtctgc atataattca aattgttctt gtagaccttt taccacgtca
     1321 gtatctttga agaataatac tgtaccataa ccatttttga aaccagctaa tttgttttgt
     1381 gtatttggga aagcttctgc tggagtcaat gggcgaagtg cttcttcagt gatttcccaa
     1441 agtttttcat gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc attgaaagca
     1501 gttgggcttt ctttaattgc ttctttgatc aaagttgtta attcttcatt agaaagtgaa
     1561 acattacgtc ctagatgata gattgaacgg cggtttttca aagtgtcagt aaaagttgtc
     1621 ataataaaat ttctcctttg atttgcgttt tgcatttttt taattgatta cgaaatgaag
     1681 tatagcacct tacaaaaagt aagtaaacag atatgctcac aaaaaataag cgaagttcaa
     1741 aaaacaattt taataagaaa aatcgttatg taagg
//
LOCUS       DAYYAG010000238         1773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000238 DAYYAG010000000
VERSION     DAYYAG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.244"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHARQ_002950"
     CDS             <1..1604
                     /locus_tag="ACHARQ_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8411318.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          285 c          359 g          488 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DAYYAG010000239         1769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000239 DAYYAG010000000
VERSION     DAYYAG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.245"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..867
                     /locus_tag="ACHARQ_002951"
     CDS             94..867
                     /locus_tag="ACHARQ_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8411319.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1038..1404
                     /gene="ssrA"
                     /locus_tag="ACHARQ_002952"
     tmRNA           1038..1404
                     /gene="ssrA"
                     /locus_tag="ACHARQ_002952"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          578 a          309 c          329 g          553 t
ORIGIN      
        1 aaccaatatg ttcaggaatt cttcacgaaa ggtttgtctt ttgttcattc tttgttaaga
       61 taaacttatt ccaataaaaa aggagtttta tttgtgtcaa attatcgtgc aatcactttc
      121 tttgatcttg atggaacatt actagatgcg aaatcaaaaa tcacacccga agtggcaaaa
      181 gctatgaacg cattaaaaga aaacaatgtt ctccccgtca tcgctacagg acgtacagaa
      241 gcagaaatcc actctatcat ggaagatgcg ggcattactt ctgcggtcac catgaatggt
      301 tctttcattc gagtagacgg cgaagaaatc tattctcaaa cattttcttc agaagaatgt
      361 caaaaaatgt atgatcatgt acagttgaac ggtcatgaac tttcttacta taacgatcag
      421 aaaatctggt gtaccggtca tagccaaaca atgatcaatg cttacgactt cattcattca
      481 gatgttccgg aaattgatcc tttaggcttt aaagaaaaca cagtaaatat gttgctgatc
      541 ttatctcaat caggtgacga atactatcac gaacattttc ctgagctaac tttttatcgg
      601 aatggtcctt actcgatcga tacagtgaag aaaggaatct caaaaggggc aggtgtcaaa
      661 cgtctgcaaa aagaacttca gctggaaaaa gttcctactt ttggatttgg cgatggtccg
      721 aatgattttg ccttgttgga agcttgcgac aacaaaatcg ctatggacaa cgcctatgac
      781 ggattaaaag aactttctac ctttatcaca aagaaaaata cagaaggcgg aattgtccac
      841 gcattgaagt attttgatct gatttaagat ctttttttga agctacctct tttcttaaga
      901 aaatcgctta ttttttctta agaaaggggc agttattata taaataatgg gatatgattc
      961 gcttttttat tgaatttatc ccaatatatg ctatactaat tttgtatgaa aaatacacgt
     1021 aacccttgtt agctaacggg gacgttacgg attcgacagg cacagtcgag cttgaattgc
     1081 gtttcgtagg ttacgtctac gtaaaaacgt tacagttaaa tataactgct aaaaacgaaa
     1141 acaactctta cgctttagct gcctaaaaac agttagcgta gatcctctcg gcatcgccca
     1201 tgtgctcgag taagggtcct aactttagtg ggatacgttt caactttccg tctgtaagtt
     1261 gaaaaagaga acatcagact agcgatacag aatgcctgtc actcggcaag ctgtaaagtg
     1321 aatccttaaa tgagttgact atgaacgtag atttttaagt ggcgatgtgt ttggacgcgg
     1381 gttcgactcc cgccgtctcc atatatgtat ggttgagaca gataaacatg tatgaaaaac
     1441 gttgttaaat caacgttttt ttattttggt caaggagaga aaagtagaga aatacaaaat
     1501 tttttgcatt gattttgcat tcaaaaaaac attcaaacat ttagaaatcc ccaatacgta
     1561 ttcaggaaaa tctattttaa ggtttacaaa aagaactttt gttcgtacac tctttttgag
     1621 gtgaactttt atgctgatgg aaggaaaaac acaattatgg tttaagcatg ttccttcaaa
     1681 taagtttttt taattattat ttgtcaaatt aagcgagaca aaactttcca tttacgtaac
     1741 tcaaaaaaac ttctaatggt gtttggtaa
//
LOCUS       DAYYAG010000240         1719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000240 DAYYAG010000000
VERSION     DAYYAG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.246"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..561
                     /locus_tag="ACHARQ_002953"
     CDS             <1..561
                     /locus_tag="ACHARQ_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411320.1"
                     /translation="VKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLS
                     DGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDF
                     SSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHY
                     NRLSEEKILLPENWAELRKLVEYGII"
     gene            548..>1719
                     /locus_tag="ACHARQ_002954"
     CDS             548..>1719
                     /locus_tag="ACHARQ_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8411321.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYT"
BASE COUNT          571 a          222 c          276 g          650 t
ORIGIN      
        1 gtaaaaaata taaaaaaaat tcccaaaata gatgaaaaca gtaaattgat gaaaaacata
       61 caatcaatct ttgggataga agaagttttt gtcggagata aatgtatctt ttttgatcaa
      121 cctttattat ctgatggtta tggcatagat gaaacagata ttttcgctaa agtaaatagt
      181 atattttctg gaaaaaaaga aactttaatt aagcttcatc ctagagcaag gacaaatcgt
      241 tatggtgaaa aaaacatatt gaattccagt ttaccgtttg aagtcttgct acttgaaaat
      301 gacttctctt caacactttt gattagtcct atatctacaa tttctttttc accatctatg
      361 atgtttgaaa agacgatcaa ctctattatg ttggctaaat taattttaaa tgaatataca
      421 gcattatctg aagataaaaa agaaacattg acaaatattg ttcttttctg tgaacattat
      481 aaccgtctat cagaagaaaa aattttactc ccagaaaact gggcagaatt aagaaagttg
      541 gtagagtatg ggattattta acacactttc caagtcaaaa ttagttaagt ccagtttctg
      601 gtatactata gggaattttt ttattaaggg aatttcattc ataactattc ctatttttgt
      661 aaatattact actcaagcag aatatggttt gattaataat ttcacttcat ttgtatcaat
      721 tttttcgatt cttgttgggc taagcctaaa ttcggcaatt aataatgcaa gttttgacta
      781 taaagatgac ataaatgaat ttatgtcatc ggtactttct ctagctacac tttcgttaat
      841 tgtgttctta ttaataggga acatatattt tttgttttca aatgcttttt ttgatatgaa
      901 ccaacttgta tttaatttga tgttattaca aagttttgct agttttgtaa ttagttatat
      961 ccagtcttat tttacgatta actttaacca ttttaaatat ttgattttat cttttatatc
     1021 tacttttttg aatattgcaa tctctgtagc tttgatgcag acattatatt ttgatgataa
     1081 atatgttggc agagtagtcg gcggaacact gtcatttgtg ttgataggtt tagtaattta
     1141 tttaagaata atgataaagg gtgctcattt ttttgataaa aaacagtgga gatacgcttt
     1201 aaaaatctct ttgccgctaa tcccacattc cttagcaaat atagttttag cacaattcga
     1261 tagaattatg attaattctt atttaggtcc tgctgcgggg ggaatatatt cctacattag
     1321 taacataggt attattctaa gcgtaatatg gatgtctact aataacgcct gggttccatg
     1381 gttctatggt gagatgagta aaaataatga aaaaaaagtg aagaaagtta gtaactatta
     1441 tttagtagtc tttactttga ttacgatgat tgtaatggta gtttcgattg atttagcaaa
     1501 atttatggct ccgaaagact attattctgg gttatcttta gtggttccga taaccttagg
     1561 ctatttcttt cagtttttat atagtttacc agttaatgct gaattttatg agaaaaaaac
     1621 atcgtttatt gcgattggta cagtagcaag tgctatagtt aatatactat taaacatgat
     1681 tttcatacct aggttaggaa taattgctgc tggatacac
//
LOCUS       DAYYAG010000241         1653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000241 DAYYAG010000000
VERSION     DAYYAG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.247"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..308
                     /locus_tag="ACHARQ_002955"
     CDS             48..308
                     /locus_tag="ACHARQ_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411322.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(670..909)
                     /locus_tag="ACHARQ_002956"
     CDS             complement(670..909)
                     /locus_tag="ACHARQ_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411323.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1195..1509
                     /locus_tag="ACHARQ_002957"
     CDS             1195..1509
                     /locus_tag="ACHARQ_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411324.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          573 a          221 c          288 g          571 t
ORIGIN      
        1 gttattatca ttttaaataa tgaaaaaaca ttaaggatgg atgaaatatg aataacgaaa
       61 ctatcatttt aggaaaaaaa tatatttgcc aacctattgg cttgaaacaa aaagtgattg
      121 gagaagtaat cagtaaatta gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg
      181 tcgatcgcaa tgaaatatta gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt
      241 atgagttgga aacaacagaa tatctatata actatagcaa caaatatgaa catactttag
      301 tttcataaag atttaaataa agtgccttca aaagcgagaa gaccaagctt ttgaaggcac
      361 tttatttagt tggcagtgat tgttcatgat ttttgattta ttcaaaaggt tcgcctagag
      421 aatctgtctc tgtaacgagg cctggtggag aaactcgata aaaacctata ctgccagaac
      481 tgtcctattt tcggatagac aaactagtag ttttaaaaag atcatccgat ccaatcattt
      541 caaaaaatgc aaaactgata tcaggatcat ttagctcagg cttgttatcg taaagcattt
      601 gtaaaggttg catttagtta ggagtaaacg cgaaactatc atcattattt gctgaagaag
      661 atgaagaagc taattcagag tttgtttgaa ataaaggaga cgtgctgaca gtttctttac
      721 cctgtttttt gcagaggtag aaacttttat atcggaagtt tctaccggag aatttttctc
      781 atttgtagaa attgttgaat cgatactatt cgaaatttgc gtatctgaag gtatgttctt
      841 tgatgcagaa gtcccacaag cagtaagtaa caataaattt gataaactga taaacagaat
      901 tcttttcatt atgtacacac tccttatagt atcgtttagc atacacctat gctcagtatt
      961 aggcaaaatc aatgcaaaac tatcaagtct tttatagtat ctattagtct tagtaataca
     1021 tatatggaga gggtgatatc ccagttcaaa ccagatacat tggttaccat ctgtattaac
     1081 gattatgcct ctaaacgctt tttttatgaa aaaatttata agtgaaaacg taggtatgat
     1141 attatctaga ggtaattttt ttgggtattt aaatagttgc taggaggata gcttttggat
     1201 ttttacagta attttatctt aataatagca atattattac ttctaaatat ttggtttttt
     1261 gataagagta gaaatgcagg gataggtttt agaaccaaac gaagtacgtc ttctgaaaaa
     1321 aagtgggtat attcacagac tattttttac ggaggggtta tttcaatcag tttactttct
     1381 tcaacacttt attcttttaa tgttatagac gtttccatgt caaattttat atctattatt
     1441 ggaatcctta tttctgcaat tattacacaa ctacttcttg tgtttgaaga gaagtcaaaa
     1501 aataattaaa gcaattatgc cttttagtac aggaattatt acttcttaca aatgtatagc
     1561 gaatgtgaag cctgctgttt taattgttct agaaaaactt ttgagtgaac aattatttta
     1621 tgttaggatg aattaatttc tctagagttt ata
//
LOCUS       DAYYAG010000242         1647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000242 DAYYAG010000000
VERSION     DAYYAG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.248"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHARQ_002958"
     tRNA            complement(20..91)
                     /locus_tag="ACHARQ_002958"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHARQ_002959"
     tRNA            complement(113..200)
                     /locus_tag="ACHARQ_002959"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHARQ_002960"
     tRNA            complement(225..298)
                     /locus_tag="ACHARQ_002960"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHARQ_002961"
     tRNA            complement(312..382)
                     /locus_tag="ACHARQ_002961"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHARQ_002962"
     tRNA            complement(384..459)
                     /locus_tag="ACHARQ_002962"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACHARQ_002963"
     tRNA            complement(478..551)
                     /locus_tag="ACHARQ_002963"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACHARQ_002964"
     tRNA            complement(564..653)
                     /locus_tag="ACHARQ_002964"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACHARQ_002965"
     tRNA            complement(677..750)
                     /locus_tag="ACHARQ_002965"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACHARQ_002966"
     tRNA            complement(764..837)
                     /locus_tag="ACHARQ_002966"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACHARQ_002967"
     tRNA            complement(865..937)
                     /locus_tag="ACHARQ_002967"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACHARQ_002968"
     tRNA            complement(957..1030)
                     /locus_tag="ACHARQ_002968"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACHARQ_002969"
     tRNA            complement(1040..1113)
                     /locus_tag="ACHARQ_002969"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACHARQ_002970"
     tRNA            complement(1124..1209)
                     /locus_tag="ACHARQ_002970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACHARQ_002971"
     tRNA            complement(1226..1297)
                     /locus_tag="ACHARQ_002971"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACHARQ_002972"
     tRNA            complement(1309..1381)
                     /locus_tag="ACHARQ_002972"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACHARQ_002973"
     tRNA            complement(1399..1480)
                     /locus_tag="ACHARQ_002973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACHARQ_002974"
     tRNA            complement(1488..1560)
                     /locus_tag="ACHARQ_002974"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACHARQ_002975"
     tRNA            complement(1564..1636)
                     /locus_tag="ACHARQ_002975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAYYAG010000243         1642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000243 DAYYAG010000000
VERSION     DAYYAG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.249"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..250
                     /locus_tag="ACHARQ_002976"
     CDS             <1..250
                     /locus_tag="ACHARQ_002976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411325.1"
                     /translation="ISNNFIRIIQNIFVLNYIIYASVLMSNYKRTKVKNYIYLLAYFF
                     FIIVCLIFINIRGTAFVNGILPLFRENIIFKAVYYFFE"
     gene            287..1225
                     /locus_tag="ACHARQ_002977"
     CDS             287..1225
                     /locus_tag="ACHARQ_002977"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM8411326.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1477..>1642
                     /locus_tag="ACHARQ_002978"
     CDS             1477..>1642
                     /locus_tag="ACHARQ_002978"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8411327.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNN"
BASE COUNT          627 a          184 c          287 g          544 t
ORIGIN      
        1 tataagtaat aattttatta gaattattca aaatatattt gtattaaatt atattattta
       61 tgccagtgta ttaatgagta attataagag aacaaaagta aagaattata tctacttgct
      121 tgcgtatttc ttttttatca ttgtgtgttt gatattcatc aatataagag gaactgcttt
      181 tgtaaatgga atcctaccat tatttagaga aaatattatt tttaaggctg tttactattt
      241 ttttgagtaa gaataaatag cacttaaaaa tagagatgag ggatttatgg gaagacaaga
      301 attatatagt ttagctatca atgaattgga aaaaagaaat cttgaaatac atgaattaaa
      361 tcaaaaaaat aattctattt taggcatgaa aatacgaaat aaaattaatc aacttaacca
      421 aatgaaaaaa cgagataatc tgttgccagc aaaatatacc cttaaatcga tgaccggtaa
      481 ggggcctatt aaagactatt caaacagaga gaatcgcagt gatgaactaa gaaaaaaaga
      541 gttaactaat caaggaacac ctaaaaggat tgctgtgtat agttgtatca ctggtaatta
      601 tgattctatt aaagaaccgg atttttctca tccattggta gattactatt tgtatacaga
      661 taatgtgatt acaagtgagc attggaatat tagagagata cctaaatata tatcggaatt
      721 aaaaaataat attttgatca atagatatat taaatttcat cctaatgagc tatttactga
      781 ctacgatttt actttgtatg ttgatggtaa tgtaacaata acttctgata ttaccggatt
      841 actgaacagt tcttccaaac ttgggttaag tatgcataaa catagtgaaa gaaaatcttt
      901 atattctgag gcagatgcat taattgcagt tggcaaaggt aataaggaaa gtattataga
      961 gcaaattagt aagtacagag aagaaggatt tcctgataat tttggattgg ctgaagccac
     1021 ggttatttta gttgatttaa ataattcaat cgctgagaat cttctagatt tgtggtggag
     1081 agagtatttg aactcagaaa gtgggcgaga tcagctcgca tttccttacg tgttgtggaa
     1141 gtccggacat gcaatgaagg aagtgactac cttaggcaat aatgtatata aaagcgctaa
     1201 attaagaatc ataagtcatt attagcccta aatgatttct aaactattct ttcaaatcct
     1261 agtaaatatt tggagataga agatttcctt tttaaagtca taaaaaatta agttatattt
     1321 tttgagtgaa tggagaagac gcatagtatc ataaacttcg ttatatctta aattatgggg
     1381 tatgcctaaa taataaatgt aatacgagta taaagaagaa agcaattttg ggtttaaccc
     1441 tattggttcc tttcagaaaa aggtggatga ataaaaatga aaatattaat aacaggtgga
     1501 gcaggattta ttggttttca tttgtctaga aaattactca aggaaactga aaatcaaatt
     1561 gtagcgattg ataatctcaa tgattattat gatgtccaat taaaagaaga tcgattaacg
     1621 atactgtacg attataataa tt
//
LOCUS       DAYYAG010000244         1611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000244 DAYYAG010000000
VERSION     DAYYAG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.250"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..782)
                     /locus_tag="ACHARQ_002979"
     CDS             complement(<1..782)
                     /locus_tag="ACHARQ_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8411328.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDV"
     gene            complement(941..1207)
                     /locus_tag="ACHARQ_002980"
     CDS             complement(941..1207)
                     /locus_tag="ACHARQ_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8411329.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1197..1553)
                     /locus_tag="ACHARQ_002981"
     CDS             complement(1197..1553)
                     /locus_tag="ACHARQ_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8411330.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          460 a          310 c          195 g          646 t
ORIGIN      
        1 acatcaaata ctctatcact caagtaactc cttaaattgt tatcactttc ccattgaact
       61 acttttctat aagttgaatt atcagttgaa aagatagaat tttcatccat aaacgcatat
      121 attagcagca atctttcatc attgtcaaat tctattggat caaaaatatc taagttgtca
      181 tcaaaatctt tatcagttcc ggtaactttt ttgttaatta ctgaaggtgt cttgtaagaa
      241 atagaatcta aatcaaagcg ttttgattct aaattatttt gtgctttctc aattaaataa
      301 ttcacttttt ttgtgagaaa ccttctgata ttgtctttgc tatatgttcc ataaaaaccc
      361 ttatcttcta aagagtccct gtacttctta acttctatat actcttcagc caaaacttgt
      421 ggaactggtt catctaagaa aaaaacccct acctcgcctt tgctattttt ataaaagtaa
      481 tcaatttctg ctatagttcc cgatctataa ttatcaactg gagaacccaa cttatggcca
      541 aaaatcgcta ataatatatc actattttct acaatttgtt tattaataac actttgccca
      601 gagctattat ttatataagt aggaatcgca ttcgtttccc atcgaattgg catcaaaatt
      661 acttttgtat tatctgtatt ttctatattc catctatata tggtttcttc tacttcattc
      721 ctctttgctg ctatatcacc tggtgatgct ataaatactt ttattagatt tatggttttc
      781 atttattgta cctttctctt tatgtctctt taaagtaatt tttgtataaa atttattgac
      841 ttttacacta taggacaact tttatctatt tagtacaaaa aggcccacta aactaattga
      901 ttagttagga gcctattaca ggtatatttt caactcgctt ttaagcaaat tctactacca
      961 gctttttgcc taatgcccta gcgatatcat caagtttatc gaaagatacg ttatcccctc
     1021 tctcaattcg agcaatagta gattgagttg tatgtgcttt ttgtgcaagc tccgcttgag
     1081 ttaggcctgc ttcctcccgt gcatgataaa gagcaaccgc agtttcgagt tttcttcctt
     1141 cacggtcata tgcttctgca aaatctttgt ctttttgctt ttcttcaatc aattttttaa
     1201 tcatcatttg tgaaccactc ctctctaagt tcttttgcat gtttaagttc acttattggt
     1261 gttttctccg tttttttagt aaatccatgg gtaatgacat accttccatt gaccacatgg
     1321 aaatagatag ctctttggat gtttgatcct accttacttc ttaattcgta caaattagtg
     1381 tctaattttt tcacccattt caaccgttga gctactagca tcccattctt ttctgtttct
     1441 tctattgtac gtaaaagctt cgcactatct ttttttggta atgtttccaa ccattctaaa
     1501 aattcatcat gaccattagg tcttttaaat gtttcaaaag atattttttc catacccaat
     1561 tatatcatat attgatttta tattatagta tataacataa aaattcgaat t
//
LOCUS       DAYYAG010000245         1611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000245 DAYYAG010000000
VERSION     DAYYAG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.251"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..626)
                     /locus_tag="ACHARQ_002982"
     CDS             complement(<1..626)
                     /locus_tag="ACHARQ_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8411331.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..1567
                     /locus_tag="ACHARQ_002983"
     CDS             914..1567
                     /locus_tag="ACHARQ_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8411332.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          579 a          225 c          299 g          508 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatacg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttatgatcg agtaatagat gtatatataa aagaacttag
     1501 aaaaaaatta caattagact gtataactac tataagaaat gtaggatata aattggagag
     1561 aaaatgaaaa aattaaagat atttccaaaa atgttcatac aaatattttc t
//
LOCUS       DAYYAG010000246         1526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000246 DAYYAG010000000
VERSION     DAYYAG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.252"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1481
                     /locus_tag="ACHARQ_002984"
     CDS             15..1481
                     /locus_tag="ACHARQ_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8411333.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          325 g          495 t
ORIGIN      
        1 ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat
       61 tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat
      121 tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa
      181 cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg
      241 gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca
      301 ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga
      361 tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac
      421 atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg
      481 tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact
      541 atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta caatacatca
      601 tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg
      661 aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat
      721 tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg
      781 tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa
      841 ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt
      901 tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac
      961 aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt
     1021 tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac
     1081 catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat
     1141 tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg
     1201 gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga
     1261 caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg
     1321 atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat
     1381 ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg
     1441 tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag
     1501 gaattgctta tttgcagcag ttcctg
//
LOCUS       DAYYAG010000247         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000247 DAYYAG010000000
VERSION     DAYYAG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.253"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHARQ_002985"
     CDS             64..972
                     /locus_tag="ACHARQ_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8411334.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHARQ_002986"
     CDS             972..>1480
                     /locus_tag="ACHARQ_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411335.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAYYAG010000248         1467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000248 DAYYAG010000000
VERSION     DAYYAG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.254"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..187)
                     /locus_tag="ACHARQ_002987"
     tRNA            complement(103..187)
                     /locus_tag="ACHARQ_002987"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Leu,seq:caa)
     gene            complement(205..276)
                     /locus_tag="ACHARQ_002988"
     tRNA            complement(205..276)
                     /locus_tag="ACHARQ_002988"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(242..244),aa:Gln,seq:ttg)
     gene            complement(286..358)
                     /locus_tag="ACHARQ_002989"
     tRNA            complement(286..358)
                     /locus_tag="ACHARQ_002989"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:His,seq:gtg)
     gene            complement(420..504)
                     /locus_tag="ACHARQ_002990"
     tRNA            complement(420..504)
                     /locus_tag="ACHARQ_002990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(468..470),aa:Leu,seq:caa)
     gene            complement(525..595)
                     /locus_tag="ACHARQ_002991"
     tRNA            complement(525..595)
                     /locus_tag="ACHARQ_002991"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Cys,seq:gca)
     gene            complement(641..712)
                     /locus_tag="ACHARQ_002992"
     tRNA            complement(641..712)
                     /locus_tag="ACHARQ_002992"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Gln,seq:ttg)
     gene            complement(724..796)
                     /locus_tag="ACHARQ_002993"
     tRNA            complement(724..796)
                     /locus_tag="ACHARQ_002993"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:His,seq:gtg)
     gene            complement(799..872)
                     /locus_tag="ACHARQ_002994"
     tRNA            complement(799..872)
                     /locus_tag="ACHARQ_002994"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(838..840),aa:Trp,seq:cca)
     gene            complement(879..959)
                     /locus_tag="ACHARQ_002995"
     tRNA            complement(879..959)
                     /locus_tag="ACHARQ_002995"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(923..925),aa:Tyr,seq:gta)
     gene            complement(961..1036)
                     /locus_tag="ACHARQ_002996"
     tRNA            complement(961..1036)
                     /locus_tag="ACHARQ_002996"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1001..1003),aa:Phe,seq:gaa)
     gene            complement(1072..1144)
                     /locus_tag="ACHARQ_002997"
     tRNA            complement(1072..1144)
                     /locus_tag="ACHARQ_002997"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1109..1111),aa:Asp,seq:gtc)
     gene            complement(1154..1226)
                     /locus_tag="ACHARQ_002998"
     tRNA            complement(1154..1226)
                     /locus_tag="ACHARQ_002998"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1191..1193),aa:Val,seq:tac)
     gene            complement(1242..1313)
                     /locus_tag="ACHARQ_002999"
     tRNA            complement(1242..1313)
                     /locus_tag="ACHARQ_002999"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1278..1280),aa:Glu,seq:ttc)
     gene            complement(1328..1417)
                     /locus_tag="ACHARQ_003000"
     tRNA            complement(1328..1417)
                     /locus_tag="ACHARQ_003000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1381..1383),aa:Ser,seq:gga)
BASE COUNT          424 a          374 c          332 g          337 t
ORIGIN      
        1 cgttttatga taatacttgt atcgaattga tttggaggta attacataaa aacaaaaaaa
       61 atatagcaac tctctataag aaagttgcta tattgataat tataccggcg gccggggtcg
      121 aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc aattccgcca
      181 cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg catgagggag
      241 tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga atgatgggaa
      301 tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca
      361 tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat cttagtcaat
      421 accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg agtccagcgc
      481 gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg taaccggatt
      541 tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata ccgccattat
      601 actattatca atctataaaa taaagaataa attatagaaa ctggggtagc tggattcgaa
      661 ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa
      721 aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc
      781 acttcgccac attcgccatg gcaggggcag caggaattga acccacacta acggttttgg
      841 agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg gattcgaacc
      901 actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc tactcctcca
      961 tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt tgctctacca
     1021 actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg acggttccga
     1081 cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc tacaccacgg
     1141 aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga
     1201 tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta cgggattcga
     1261 acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa
     1321 tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat ctacatgatt
     1381 tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag ctcaatactc
     1441 tttctcaaaa tgagaactcc ggcagta
//
LOCUS       DAYYAG010000249         1436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000249 DAYYAG010000000
VERSION     DAYYAG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.255"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..253)
                     /locus_tag="ACHARQ_003001"
     CDS             complement(<1..253)
                     /locus_tag="ACHARQ_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411336.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSD"
     gene            complement(255..>1436)
                     /locus_tag="ACHARQ_003002"
     CDS             complement(255..>1436)
                     /locus_tag="ACHARQ_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8411337.1"
                     /translation="IIINRENFLIPSIYNFGYLLITLIVIFPADFSQSEEEKKKKMVY
                     FNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAM
                     FGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLL
                     LFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVI
                     ESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNS
                     DAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKT
                     FFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKE
                     N"
BASE COUNT          560 a          234 c          173 g          469 t
ORIGIN      
        1 catccgagaa aaattgatta tggttagtta acaagttaat tttacgttta aaggaagaac
       61 cgattttccg gtgattcaca aaaacaacat cattaaatag ccctaattct tttatacgct
      121 cactatatct tctagcgttt tcaaattcag aataaataaa taaatctcct tttgattctc
      181 ggtaaaaatt ttgataaaca tttaatatat taatcaaatg taaaggagtc caagcaatga
      241 aagctgtctt catattaatt ctcctttttt aaatacaagt aagaaatgct tcccaaataa
      301 taccagaaaa caattgaaat gacatcgcgc ttattgaaaa gtacatgagc ctccaccatt
      361 tcatctataa aaatagatag tataaaaatt aaaacaatac caagaaacgc ataattcact
      421 ttacttctaa aaaatgtttt gagtaaacta aagatattta acacatagaa agcatagata
      481 catataaaag caattatacc acttgataca agtacttgaa gaaatccatt atgcatgttt
      541 ccatgtgctc tttttagttc attcacatct gtagtatcaa gatctacttt tttcaaaaat
      601 tttgcatcgc tattcccata gaaatcggtc tctccataac caaataacaa agttggtctt
      661 ataactttaa gcgcagcttt ccaaatcgta aaacgaccag ctgaaatttc ggagttatca
      721 ctagaatgct gtaatattac ttctttttct ggaagtggag ttttatctga ttcatttgac
      781 tctataactt ctacttttct atcaatcgat acaggaattg ttgctaaaaa atccttagca
      841 taattagtac caaaagaaaa atataccaat agaactaata atatgcttac catccatttg
      901 atttttacag gtagtacttc tctaatgctc tttctaaatt ttgaattggc aaacaacaaa
      961 agaaataata ctaaaaaaga acaaattact acctgggcac ctctagacga agaaagtgta
     1021 atataaaata tttgtacgat aaaattcatc atataaaaaa cacgaatcgt ttttgttatt
     1081 tttgaaatta atacactaat taaacttaaa ataatagaag tatatccaaa catagctcca
     1141 acatttggac ttgtatagac accaaataac cgatgttcaa taaaaccttg tcttgcaagt
     1201 aatcctggcg ttcctgttgg gacatgataa gaaatcaaaa aaataaattg atagatagat
     1261 attagcgcag ctaaaaaaat tatgataatg aaaacattat taaaatacac catttttttc
     1321 tttttttcct cttcactttg tgaaaagtct gcaggaaaaa ttactatcaa agtaatcagc
     1381 aaatatccaa aattataaat agatggaatt aaaaaatttt ctctgttaat gattat
//
LOCUS       DAYYAG010000250         1396 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000250 DAYYAG010000000
VERSION     DAYYAG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.256"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1396
                     /locus_tag="ACHARQ_003003"
     CDS             <1..>1396
                     /locus_tag="ACHARQ_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411338.1"
                     /translation="DDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFDKESMIGDKV
                     AKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDDVPDDSEQIN
                     KKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMNEFLGILSNA
                     QILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLVQELANYNEY
                     TGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKDNSDNWLMKS
                     DKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKWEETANAYKA
                     ERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFINWFYDNYNEK
                     LSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTALKKIDCSSF
                     AEERDFLSFILRCGV"
BASE COUNT          501 a          203 c          256 g          436 t
ORIGIN      
        1 tgatgatgaa aaaatatcag agaaaattgt ggatggaaat gatgtattga tagaaaaaag
       61 tgatgttact ggtcaatcac cgtctgaaga atattttgtt tttgacaaag aatctatgat
      121 aggagataaa gttgctaaaa ttgcactagc cgtttctaaa gattctgatt acgattactc
      181 caaagaaaaa ctatattgct actttccagt acgtaatttt ggtacaccaa ttcatgcact
      241 tataaatgca ccgtttataa caaataattc acgtgatgac gtcccggatg atagtgaaca
      301 aataaataag aaaatattta ttgaggtgct caaatttatt tctgaagtag cagaaaaatt
      361 atctaagcct gaatatgaag acatagcatt aaaaatggta actcctttta aagagaataa
      421 actatgggac ttagatgtat ttgacttaat gaacgaattc ttgggtattt tatctaatgc
      481 ccaaatacta ccgactgtca attggcaata tatttctatc aaagataatc caaaactttt
      541 ccttcaagaa ttccctgagg agctaatagg tgagacattt tccaccttgt tgcaattatt
      601 agaaacaaac gaaaatgacc aactagtaca agaacttgct aattataatg aatacactgg
      661 cctgtcgttt gttgtagatg aactatctga taaaatcaac attctttcat cttcttggga
      721 aaatagaata aggacaaaag tgttcttatg gtggagtgag caatataaaa gttctccgat
      781 tgtccctaag ctattaaagg acaatagtga caattggttg atgaaatcag ataaggtatt
      841 cttaccgacc gatacaggtg ttactgtgct tccaaaagag ctgtcttctt gggttagttt
      901 aagcattttg aaacaaacat atgttgatga gttaatttat cagattaaga cttcatcaag
      961 ttggaaaaat aaatgggaag aaacagctaa tgcatacaaa gctgaacgta caggagataa
     1021 acgtattcta gatgcattca gtgaaaaata tcttgcaatt gaattcactg agcaaagcag
     1081 ttcagatttg atcattggaa cgattaatcg acaaattgac actgttgaaa aatctatcag
     1141 tttcattaat tggttttatg ataactacaa tgaaaaacta tcagctggaa gtgagctgtc
     1201 taaagttcca tttaatcttc caaacgttgc tggtgacgtt caaccttcca ataaaatgta
     1261 tattggaaaa gagtatggca atgaattagg agataagctt tttcaagata cagattacac
     1321 ggcattgaaa aaaatagatt gctctagttt tgctgaggag agagattttc tgtcatttat
     1381 tcttcgatgt ggtgtt
//
LOCUS       DAYYAG010000251         1380 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000251 DAYYAG010000000
VERSION     DAYYAG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.257"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..1033
                     /gene="scrK"
                     /locus_tag="ACHARQ_003004"
     CDS             161..1033
                     /gene="scrK"
                     /locus_tag="ACHARQ_003004"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8411339.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          439 a          235 c          314 g          392 t
ORIGIN      
        1 attgtatgaa aaattattag aattttaaaa aatgataaaa attcaaaaag aaaaaatagt
       61 agttatttac ttgtcatgtt actaaataaa aaaataattt aaatatgata tatagtcatt
      121 tatttagtga tttcattcta aaataaaggg agaaatgtct atgctatacg gtgcaattga
      181 agcaggcggc acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag
      241 agtgagcttt ccgacaacga cacctgagga aacaatgcct ttagtcattg acttcttcaa
      301 acaataccaa gcagatttag cagggattgg gattggctca tttgggccta tcgatatcca
      361 tcgcgattcc gccacttatg ggtacattac ctcgaccccg aaattggcat ggcaaaattt
      421 tgatttcatc ggtaccatga aaaaagaatt tccgatcccg atttcttgga caacagatgt
      481 caatgcggct gcctatggtg agtatgtctt cggcagtggc aaaggattga gcagtgtcgt
      541 ttactatacg atcggtacgg gtgttggcgg cggtgcctta caagaagggc gctttatcga
      601 agggtttagt catccagaaa tggggcatat gctcgtcgtc cctcatccaa aagatgactt
      661 tgctggcagc tgtccatttc atggcaactg tttagaaggg atggccgctg gacccgccat
      721 cgaaaaccgc ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt attgggaaat
      781 cgaagcctcc tatattgctc aatgtgccta caatacaacc ttgatgtttt ctccagatgt
      841 gatcattttt ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc
      901 atttgcaaaa ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat atatcgtgac
      961 cccagcatta gaagacaacg ctggaacgct cggttgtttg gcattggctc gagaagcggt
     1021 cttacattca taaacgaaaa aaggctcttc gacatttagt gttggtagag aaagatacaa
     1081 ctgaaaattt agtcataacc agttaggcag agtgcttaac tggtttttgt tttacttgga
     1141 agatcaagcc ttgaatgaga taaattcttg ctcgaaagtt atggaaattt cgatagccgt
     1201 aggccacttg tattgtgcaa tagtttttta caaaaaaaag ggggtgcgga tatttttttg
     1261 gaccttttta acaaataggt tctgttgcaa agttttaaat ctactatcag ataaggtaga
     1321 ataatagaaa aagatagcag gaggaatgac gatgagtcat tttaaaggaa agcaatttca
//
LOCUS       DAYYAG010000252         1374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000252 DAYYAG010000000
VERSION     DAYYAG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.258"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..399
                     /gene="spx"
                     /locus_tag="ACHARQ_003005"
     CDS             <1..399
                     /gene="spx"
                     /locus_tag="ACHARQ_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFM8411340.1"
                     /translation="YTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAA
                     TEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFN
                     EDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            413..769
                     /locus_tag="ACHARQ_003006"
     CDS             413..769
                     /locus_tag="ACHARQ_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8411341.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          500 a          207 c          256 g          411 t
ORIGIN      
        1 tatacttcgc ctagctgtac ttcctgtaga aaagcaagag catggttgca agcgaatcaa
       61 cttgaatttg aagagaagaa tatttttgcc gaaccgttaa cagaaagtga gatcaaaaag
      121 atcttggcag cgactgaagg aggagttcaa gatattattt ccactcggtc gaaaatttac
      181 gaaaagctgg gcatagattt taacgaatta actttaaagc aaatgatcgt cttattcaaa
      241 gaatatcctt cattattgag acgacctatt ttgattgatg aaaaaagaat actaatcgga
      301 tttaatgaag atgagatccg gtcatttgtc cctcgtgaaa ttcgatgtgt tgccagaaat
      361 ctgatctatt tacaaaacaa taacatctta aattcatagg gagggagcct tcgtggaagt
      421 gaaagtctat ggatcagcgc actgtgtaac aagtctgaag gcgaaacaat ggttagaaca
      481 ccatcaattg ccttatcaat ttgttgactt agaagaacgc gatttaagta cagaagaagc
      541 agaagaatta tgcagttttg aagaagtgca agcagaacag ctatttgcga cttggtctga
      601 ggcttttcac aacattgcga ttgacctttc ctgtgtagga aaaaaacaac tgattttttt
      661 atgcacacaa cataccaatc tcctcagacg tcctttgatt attatcgatg aagttctatt
      721 tattggatac aatgagcagt tgatggaaca gcttatctta aataaataga ctttaactgt
      781 tttaatcaag tcactgagca tttcatacag ttgtaacagt cgaacactta gcatcaagcg
      841 tgttatgtgt tctttttttg actttatttt tctactatag agacatgctc aatttatgtg
      901 gtcaaaattc aaatctaggt ggggatatag agttaaaaat atattgacat gtgtaaattt
      961 acatgatata ttcatttctg ttgttttaag tagcaaagca ataaaatagt attttaaaaa
     1021 acatataaaa tagttgttga catgttagaa atgacatgat aagataactg agttgttaaa
     1081 aaagaaccac ttgtgaagct tgattctatc gaaaaaaact tcaaaaaagt tcttgacaaa
     1141 taacaaatga taagttatga taaatgagtt gctgtaagat ttcgaaaaac gaaataaaaa
     1201 aaacttttaa aaaagagttg acaattactt gtctctctga tatgatataa aagttgctac
     1261 ggcagccaaa aacgaatcgg ggaacactcc tgatgaagta gacctttgaa aactgaacaa
     1321 agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaaca aaca
//
LOCUS       DAYYAG010000253         1368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000253 DAYYAG010000000
VERSION     DAYYAG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.259"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1368
                     /locus_tag="ACHARQ_003007"
     CDS             82..>1368
                     /locus_tag="ACHARQ_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8411342.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAYYAG010000254         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000254 DAYYAG010000000
VERSION     DAYYAG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.260"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHARQ_003008"
     rRNA            <1..1355
                     /locus_tag="ACHARQ_003008"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYAG010000255         1334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000255 DAYYAG010000000
VERSION     DAYYAG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.261"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1193
                     /locus_tag="ACHARQ_003009"
     CDS             153..1193
                     /locus_tag="ACHARQ_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8411343.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          542 a          183 c          212 g          397 t
ORIGIN      
        1 ggtagtacat aattcatttt atttctgatt ttaggtagta tttaattaaa aaacagtttt
       61 aatgcgtttg cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat
      121 aaataaaaca acgtgaaagt agaggatcta caatgactga ttttaaattt tttaaggcgg
      181 atagagttta caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat
      241 acaaaaaaat gaaagattca actaagattg cctatatgct tttaaaagca agattagaga
      301 tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa
      361 atgaactatg tagagtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt
      421 tggaatcctt tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa
      481 ataatcctaa tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac
      541 tcgaaaaatt tgatagagag aatagggaaa acgttgataa atcagagggt atgaaaatca
      601 tacccaccct cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa
      661 tcataccgtg ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata
      721 ataatatatt agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc
      781 aattgttgtt agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc
      841 ctgaacgagt attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa
      901 ccgtccagac tattcataat gcaaagaaaa aagctgaaat agaaagtgat atttcgatag
      961 tttttgaaga actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt
     1021 taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg
     1081 tctatgtaaa aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac
     1141 gtttgaatta tgcaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta
     1201 ctgacaaagt gattgttgct tcatggagta acattaaaat taaaaatttt ttattattct
     1261 aattatatta cgaatattac aaaataatag ctattttaat agaatttata agttaaaata
     1321 ttatgataaa aatc
//
LOCUS       DAYYAG010000256         1324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000256 DAYYAG010000000
VERSION     DAYYAG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.262"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHARQ_003010"
     CDS             278..820
                     /locus_tag="ACHARQ_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411344.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHARQ_003011"
     CDS             976..>1324
                     /locus_tag="ACHARQ_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8411345.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAYYAG010000257         1315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000257 DAYYAG010000000
VERSION     DAYYAG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.263"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1064
                     /locus_tag="ACHARQ_003012"
     CDS             57..1064
                     /locus_tag="ACHARQ_003012"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8411346.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1076..>1315
                     /locus_tag="ACHARQ_003013"
     CDS             1076..>1315
                     /locus_tag="ACHARQ_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFM8411347.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQS"
BASE COUNT          522 a          167 c          246 g          380 t
ORIGIN      
        1 ttttaaagat taagaatcga attatagatg actataattc ataggaggag atataagtgt
       61 ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt gaaattgttc
      121 gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt gaagaaaaac
      181 aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta attggtgata
      241 tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt tttcatgctg
      301 ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta aaaacaaatg
      361 tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa cattttgtag
      421 gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca aagttattga
      481 gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca atattttctt
      541 gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt tttagtaaac
      601 aaataaaaaa caataaagat attactttga cagatgttga tatgacacga tttatgatgt
      661 caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa tttggagaaa
      721 catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca ttaataaaaa
      781 tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta ggtatcaggc
      841 caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc tcatatgaaa
      901 atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg tacaaagatt
      961 atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg agtggacagg
     1021 aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt ttatgatgaa
     1081 atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc tagatttagc
     1141 tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat ttcaaacatt
     1201 taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga aggataatct
     1261 taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc aatct
//
LOCUS       DAYYAG010000258         1305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000258 DAYYAG010000000
VERSION     DAYYAG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.264"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..513
                     /locus_tag="ACHARQ_003014"
     CDS             193..513
                     /locus_tag="ACHARQ_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8411348.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            531..962
                     /locus_tag="ACHARQ_003015"
     CDS             531..962
                     /locus_tag="ACHARQ_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8411349.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            1092..>1305
                     /locus_tag="ACHARQ_003016"
     CDS             1092..>1305
                     /locus_tag="ACHARQ_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8411350.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGN"
BASE COUNT          482 a          181 c          217 g          425 t
ORIGIN      
        1 agacataaaa acattgattt taaagctttc ttggcggtct gcatttacat atcaatagtg
       61 ttttttgttg tctttttagt tgttaaatct gtttacaaga tatagtatag ctcattactc
      121 agaattagtc aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag
      181 gttgttttat cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata
      241 tctatacggc agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa
      301 aataatactc ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta
      361 gactaccttc taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac
      421 gatactgttt tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt
      481 gcaattgcta aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga
      541 aattgaaaga aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc
      601 ctggtttcta ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa
      661 ctgcagaagc ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat
      721 tttcagtttt gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata
      781 aaatagctat tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc
      841 agtttaatct cgaaaaattt atggagtatt attctattca gaataagctc agatacactt
      901 gttatgctgt ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat
      961 gaatacatca agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct
     1021 gcaacacggg ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg
     1081 aggaagaaaa aatggaaaaa gagattttaa taaaatctcc aggaaaaacc ttgattaaag
     1141 taactgatga ttctatttca attattagaa aaggatttat aaatttagtt aatcaaggta
     1201 ttaaaggaga aaaaactatc ccctttaaaa atatatcagc cgtacaatta aaaaaaccag
     1261 gtatgagtaa tggttatata caatttacct tgctaggcgg aaacg
//
LOCUS       DAYYAG010000259         1225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000259 DAYYAG010000000
VERSION     DAYYAG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.265"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..712
                     /locus_tag="ACHARQ_003017"
     CDS             <1..712
                     /locus_tag="ACHARQ_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFM8411351.1"
                     /translation="TPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLL
                     STGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSD
                     HSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLG
                     DGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGK
                     KVKRPISADSVLLEEDVE"
     gene            709..>1225
                     /locus_tag="ACHARQ_003018"
     CDS             709..>1225
                     /locus_tag="ACHARQ_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8411352.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVG"
BASE COUNT          406 a          162 c          268 g          389 t
ORIGIN      
        1 tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa
       61 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
      121 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
      181 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
      241 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
      301 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
      361 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
      421 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
      481 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
      541 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
      601 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
      661 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
      721 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
      781 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
      841 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
      901 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
      961 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
     1021 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     1081 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     1141 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     1201 tcttggagag tttttcccgt cggag
//
LOCUS       DAYYAG010000260         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000260 DAYYAG010000000
VERSION     DAYYAG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.266"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHARQ_003019"
     CDS             <1..1076
                     /locus_tag="ACHARQ_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8411353.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYAG010000261         1175 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000261 DAYYAG010000000
VERSION     DAYYAG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.267"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..561
                     /locus_tag="ACHARQ_003020"
     CDS             <1..561
                     /locus_tag="ACHARQ_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8411354.1"
                     /translation="PVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKI
                     DYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNS
                     KYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKE
                     DYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            596..>1175
                     /locus_tag="ACHARQ_003021"
     CDS             596..>1175
                     /locus_tag="ACHARQ_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8411355.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTEN"
BASE COUNT          452 a          178 c          192 g          353 t
ORIGIN      
        1 cctgttttct ccattgcaga tttacgaaag aaatttagta ttgagaaaaa atataaagct
       61 tttcgagact ttgaaagacg tgtaattaaa gaaccgattg cccaactgaa taaatacact
      121 gattttaaaa ttgactatga gcttatcaag aagggtcata cttatacaaa cataaagttc
      181 ttgcttacac gtaccatgtc agttccggta ccttataagc aagaacaaag agaccctgct
      241 tatttaaaag gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg
      301 aatagcaaat acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc
      361 gatcttaaac ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc
      421 aaagatagag gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag
      481 gattacacaa taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct
      541 tatgtgaacc tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa
      601 aaaagatgaa ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga
      661 tttaaagtcg tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa
      721 gaaaacaggc gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca
      781 aaatcttgaa acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga
      841 aatttcgaat ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat
      901 tcattgggat ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt
      961 aattgtctca atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca
     1021 aaaaaaatat agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat
     1081 cgccaaaaat aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat
     1141 agttaaaatt cgtgagatta cgaaaacaga aaatg
//
LOCUS       DAYYAG010000262         1156 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000262 DAYYAG010000000
VERSION     DAYYAG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.268"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..118
                     /locus_tag="ACHARQ_003022"
     CDS             <1..118
                     /locus_tag="ACHARQ_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8411356.1"
                     /translation="LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            123..1070
                     /locus_tag="ACHARQ_003023"
     CDS             123..1070
                     /locus_tag="ACHARQ_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8411357.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          371 a          171 c          245 g          369 t
ORIGIN      
        1 tctaatttta gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa
       61 atttttagaa gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa
      121 ggatgagctg ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc
      181 atttaggaga agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa
      241 atcttgatat gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga
      301 tggaagagat catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta
      361 gtgtggcaga ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat
      421 taatgatttt agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt
      481 cttcagcggc tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc
      541 gcccattgac gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt
      601 gtcatttata tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc
      661 aaaatccgaa ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac
      721 aaatcgtagg agaagcaact tcatttacat tatttggtga tggaagccag tctagagact
      781 ttgtttatat agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac
      841 tggggaaaca attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg
      901 caatcaatga aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg
      961 atatccatga ctctctagca gatattacta agatccagtc aattggctat caaccgaaat
     1021 atggtatttt aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa
     1081 gataagatta gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa
     1141 tcaaatctgg gataga
//
LOCUS       DAYYAG010000263         1154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000263 DAYYAG010000000
VERSION     DAYYAG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.269"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..120)
                     /locus_tag="ACHARQ_003024"
     CDS             complement(<1..120)
                     /locus_tag="ACHARQ_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8411358.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLV"
     gene            complement(250..834)
                     /locus_tag="ACHARQ_003025"
     CDS             complement(250..834)
                     /locus_tag="ACHARQ_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8411359.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          406 a          203 c          148 g          397 t
ORIGIN      
        1 tactaaaact gtagcaataa tgaaacaccc cacttgaaat atatttccct ttcctaaaaa
       61 aataatgacc ggaaaaaaaa tatttaaagt aatcaaggtt aacggattga tttttgacac
      121 tctttcacct ccaaaaaata agatcgcact gttatcttaa gctgataaaa atgcaccgtt
      181 attcagataa cagtgcgatc ttatcaaaaa gcataactga gaacaattct caatgcaagc
      241 tttttttaat taactcagta attcaatcca cccaggaaca gaaaattgat acaatcttct
      301 ggttaatagc atagtctttt cttttgaata gctatgcttc aatatatcat agaatgtagc
      361 gtagaatgag taaatgtaga agtggatttc ttcttcatta aagactaaat tttgattgat
      421 cattccgata gtctttaact tatctagaca acagactgtc tttttcgtca caaatgatgt
      481 aattttttca acaatatctt gatatttggt tccagctgat tggaataaaa tcaaatcgaa
      541 tatacgttta ttctcaaaaa tataattaat aaatggatct aattcattga agttctctct
      601 ccaacttttc tctatatcta tattttctaa gtttaagagg tatttggtaa actcttcatt
      661 atacatatta ataagcgcat taatatgcgg ctgaataatt tcttcaaata acgcttcctt
      721 agaagtaaaa tgtttataaa aagcacccgt agttacgcct gcggtcaggc acagttgcct
      781 tatcttagtt tgctgaaatc cttctttttc aaaatggctt agtccacttt ctattaactt
      841 ttggtgagtt ataatataat ctctggtttc tatgggtact tcctcctaat aagatgaatt
      901 aactttatta tcttacacag gacttgaagt ttcaatcgtt aacgtttatt actgaactca
      961 ttccttagag ctaaactaat gagaaagagc agaataactg atacaaaaag caatcctaca
     1021 agaattaata gactcatcta aatcattcac cttttctaaa ataatccgag aaagatttaa
     1081 aaaaattata tccagctatt aggataagtc ctatcataat cactccaata gccattttct
     1141 tatcctccct aaaa
//
LOCUS       DAYYAG010000264         1149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000264 DAYYAG010000000
VERSION     DAYYAG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.270"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1149
                     /locus_tag="ACHARQ_003026"
     CDS             <1..>1149
                     /locus_tag="ACHARQ_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411360.1"
                     /translation="ISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDSKVTI
                     EKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFGVFNG
                     FSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIGKRFF
                     SDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFGLAEA
                     HFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDFEMTS
                     FTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVSICPVS
                     YFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLS"
BASE COUNT          394 a          170 c          210 g          375 t
ORIGIN      
        1 aatttcagaa gaagcagaga tccatgtcga acgtctgaat ttgacggata tcaaaaattt
       61 aagaagaatc tactttcaac gatcaaaaat tgctcgactg ttaacggaga tcgtggattc
      121 aaaagtaacg atcgaaaaat ttgcagagaa tcatttttta agcatatcaa gagcctatgt
      181 aaaaagaaga gaattagtct cttttcttga acagcatcaa ataaagttaa agaaaaacat
      241 attagttggg gaagaaataa acatacggaa cgtattattt ggggtatatt tcggggtctt
      301 taatggtttt tctatgccat ttagagaaag cgtcatgaat gaagcaaaga gtatcgtgca
      361 atattttgaa tatctgttca atttaaaact gtctgcaaca aaacgaatca aattcaattt
      421 gctaaccact attagtttgt tgagggccta tgggaatcaa ccaatcggaa aacgtttctt
      481 ttcagatgaa tactttaaga gctcagaagg aatgaagatc atcaaggatt tatcacatat
      541 ttcttttctt catatcccta aagagcagat cattgatgag atttcttata tcatgttgtt
      601 tttgatcacg gaagaagaat ttgaattaga tggcgcattt ggacttgcgg aagctcattt
      661 tcatgatatc cacatgatta gtcaaaaaat gtcagtggaa ttgattgatc aattaccatt
      721 ttcgattgaa atctctgaag aagaaagact atatatacga aaagaacttc gtatgggtat
      781 gatgcatgtc aatcgaaaaa attggatatt cgactttgag atgactagct ttacgtctaa
      841 aaagcagcta cagtttttct tcgagacgta tccaatattc agtacagcaa tccaaaaggt
      901 catacaagag aataaacgct atttcttggg aaaagatgaa cgtttcatga ttcgcttatt
      961 ttatgattat ctctttttat tggtcagtat ttgtccggta tcatattttg aagcaccgat
     1021 tcatgtctgt attgattttt cgcaaggatc tgcttatacc gattacatta tttctcaagt
     1081 gaaaggattt aaaaattaca atattgtgat tgagagtcga atcacttctc gtacgaatat
     1141 ttatttatc
//
LOCUS       DAYYAG010000265         1135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000265 DAYYAG010000000
VERSION     DAYYAG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.271"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..543)
                     /locus_tag="ACHARQ_003027"
     CDS             complement(<1..543)
                     /locus_tag="ACHARQ_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8411361.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTN"
     gene            complement(653..>1135)
                     /locus_tag="ACHARQ_003028"
     CDS             complement(653..>1135)
                     /locus_tag="ACHARQ_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8411362.1"
                     /translation="GGAFGHSISPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVLLLF
                     FTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWF
                     FTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK
                     "
BASE COUNT          411 a          227 c          160 g          337 t
ORIGIN      
        1 atttgtattc tctgtcattt tctttaacaa gaatacacca attactaaca tggcgattag
       61 aaccaatcca attttaaagt taatgaaaaa gcctaaggca actgataaaa caggtgttac
      121 tatggcttga gcactatctg gaatcaagtg agcaattgcc atatgtgttt tagccgcgtt
      181 gtcatcaatt gtctttcgaa tatatcccga agaatttaaa tcaaaaaaac gaaaacttgc
      241 ttctgttaaa ccgctaattc ccttttttct caaattagtc tctaagcgaa acccaaggat
      301 atgagaaaat aagccagaaa aaatataaaa taaagctcct aaagttaacc aagcaacagt
      361 ttgaaccgca taactcttag ctaaatattc atctcctaca ataattagag actgaagaaa
      421 tttgtaaata ctgtaatatc catagactgt taatacgaca gaaaaatcag aaaataaagt
      481 ggctaaccat ccgtagtatt tcattttagg gacataacta aataattttt tgtatacttt
      541 cattttttcc tcccccgcat tcaatttctt ctcttattcg tttacaactg ttaatgcttt
      601 ccgtaatagc gtaaattaga gatgtacaaa cagataaaag gtaaactacg ttctattttt
      661 tgtatttttt taagactttt cttccaatca atacaccaat catagataaa cttgctacaa
      721 ctaacactga aatgattccc catataccta ccgcatagtg gatcattgca tccattgttg
      781 cagcagattg tcctttatct atccaatctt gacgaaaacg atctgtaaag aaccatatag
      841 gaatccactg tccggcagca ctgcctaatt gcattagagc ccaagacgca acttgaggaa
      901 ttgctcgatc atatccaaat acagatagaa taatatcagc taagatcgcc atactaatcg
      961 aactgataaa ccaaggtaag tacgccccgc ccattaacgt aaaaaataac aataaaacaa
     1021 ttgtgtaaat agtaaaaatt ccttttctag ggtagttata gctaatataa acaaatatga
     1081 ttccagataa taatccaaaa atcccggggg atatgctgtg accaaacgcc ccacc
//
LOCUS       DAYYAG010000266         1069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000266 DAYYAG010000000
VERSION     DAYYAG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.272"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHARQ_003029"
     CDS             <1..969
                     /locus_tag="ACHARQ_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8411363.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAYYAG010000267         1066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000267 DAYYAG010000000
VERSION     DAYYAG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.273"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHARQ_003030"
     CDS             complement(53..787)
                     /locus_tag="ACHARQ_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8411364.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          376 a          216 c          189 g          285 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcaga
//
LOCUS       DAYYAG010000268         1041 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000268 DAYYAG010000000
VERSION     DAYYAG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.274"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHARQ_003031"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHARQ_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHARQ_003032"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHARQ_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          361 a          180 c          153 g          347 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca a
//
LOCUS       DAYYAG010000269         1032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000269 DAYYAG010000000
VERSION     DAYYAG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.275"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            407..>1032
                     /locus_tag="ACHARQ_003033"
     CDS             407..>1032
                     /locus_tag="ACHARQ_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8411365.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          327 a          192 c          193 g          320 t
ORIGIN      
        1 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
       61 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
      121 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
      181 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
      241 aatcaagttt tttgttgcga aaaaacggtt ctataatatt ttgtgttggt ggaaaatcga
      301 aagcgatttt ctactaacac ttttttcttc gtttcaactc aaaaagagac tcacttttgg
      361 taaaatttag tcgaccaaaa cgtcacccta ccaaaggagt ctctcaatgg ataatcatac
      421 tagaaaatta ctcggattaa cagataaaca tttatttttt gatgaagaat ggctgattga
      481 aaaagaatat aaaggcgttc aagcgcagtt tattaaaggg agattggatg actcaccttc
      541 ccattgtgaa cactgtggat cgattcgtat tatccgcaac ggttcctata cgacacgcac
      601 tcagatgttg aaagtcaaag aaaagttgac tattttagaa ttaaaacgta cccgttttct
      661 ctgccatgat tgtggacgga ccttttctgc gaaaacggac ttagttgatg aacatcatca
      721 actaacaaag gaactgaagc aggcgattct catggaactg tacgaaaatc aatcacgtaa
      781 actaatcgct aagaagtact tcgtctcgga tgggacggtg acacggatct tgcgtgaagc
      841 aacgaagcat taccagccac gtatgaactt ccttccgacg gtgttgtgta tggatgagtt
      901 caaatcaatg aagtctgttt ctggttccat gagttttatt tgtgttgatg ggacaaccaa
      961 tcagctattt acgatccttg aagatcgcag actttacaaa ctaactcaat acttcctgcg
     1021 ctttcctaga aa
//
LOCUS       DAYYAG010000270         1008 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000270 DAYYAG010000000
VERSION     DAYYAG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.276"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..472
                     /locus_tag="ACHARQ_003034"
     CDS             <1..472
                     /locus_tag="ACHARQ_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM8411366.1"
                     /translation="DAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVYTSNELLSS
                     LEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPV
                     LIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            483..>1008
                     /locus_tag="ACHARQ_003035"
     CDS             483..>1008
                     /locus_tag="ACHARQ_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8411367.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFK"
BASE COUNT          343 a          139 c          170 g          356 t
ORIGIN      
        1 tgatgctatc aagatagagt tttttactat attttttgcg acatccttat atattgtatt
       61 tcgaatgttg ccggtcgtaa tgatctcatg gttgttggtt agtgtctata ctagcaatga
      121 attattatcc tctttagaaa aaatacattt accgaaaaaa ataatgttaa gtattacagt
      181 agtgatcaga ttctttccga cttatcgttc tgaaataaga atgattaaag aatctttaaa
      241 aatgagaaac attcgcttgt ctttttttca gcctctgcaa tatttggaat ttctgttagt
      301 tcccgttctt atacgtgcaa ctatgattgc tgaagaaatg accgctaatg caataacaaa
      361 ggggatagaa tctcctgtga aaagaagttc ttactataaa acaaacatga gcttattaga
      421 ctatatcatc ctgttttccg ttactatttt tttcttaagt attttgattt gaggtgaaag
      481 aattgtttga atttaacaat gtttcttttt catataagga tgttaaggaa caagcgcttg
      541 ttgaccttaa tttttcaata aaaaaaggca gtcttacatt gattactggt tcttctggga
      601 gtgggaaatc cacactatta aagttaatga atggacttat tccaagactt tatgacggaa
      661 aattggttgg ggaaatttta tttgaaaata actcgctaca atccttaaca caagaaagta
      721 tatcaaaaaa tattggctac gtaagtcaag atcctagagg acaattcttt acaacaaata
      781 caacgagtga gttagtattt tcgatggaaa actttggatt tacaaaagag catatggata
      841 aaagaatcaa tgaattagct gaattattag atctttcaaa tatattagat aaaaacattt
      901 tttcgctatc aagtggtgaa cggcagaaaa tttctattgg atgtagttta tcgctaaact
      961 ctaaattaat tatattggat gaaccgtctt caaatttgga cttcaaag
//
LOCUS       DAYYAG010000271          987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000271 DAYYAG010000000
VERSION     DAYYAG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.277"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..190
                     /locus_tag="ACHARQ_003036"
     CDS             <1..190
                     /locus_tag="ACHARQ_003036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411368.1"
                     /translation="IGGFKMSHTLLIKDTLNILDLNINIHFEENCLTKEKYKGQICLI
                     YKGTLQYTPEEYPHLPIY"
     gene            250..483
                     /locus_tag="ACHARQ_003037"
                     /pseudo
     CDS             250..483
                     /locus_tag="ACHARQ_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(531..650)
                     /locus_tag="ACHARQ_003038"
                     /pseudo
     CDS             complement(531..650)
                     /locus_tag="ACHARQ_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
BASE COUNT          357 a          196 c          128 g          306 t
ORIGIN      
        1 cataggagga ttcaagatgt ctcatactct gcttatcaaa gataccctaa atattctaga
       61 cctaaatatc aatatccatt tcgaggaaaa ttgtttaact aaagaaaaat acaaaggaca
      121 aatttgcttg atttacaaag ggactcttca gtacacacca gaagaatatc cacatcttcc
      181 aatatattaa aagaaccttc ttgaatcatt gagtgaaaga aaagagattc gtcactattt
      241 gaggactgtt taactagaga cattagcgat ctgcctgaaa cctatacaac cactttacga
      301 acgtttaaaa attttctgcc acagattcaa aatgcgctta actgcagcta ttcaaacaga
      361 ccattggaat gtttaaacaa ccacatcaaa gtattaaaac gcaatacgta tggctttcgc
      421 agcttttaca atttcaaact acggatcatg attcgtcatg gcaaaacatt tttaaccaaa
      481 taaacagaag aatgtgggat aaatctaaaa atcagttttg ctacagactc tcgagtaaac
      541 ggcgggagca gaagcaactc cttcggaaat aagccgaaat tcacaaaaat ttgaaaagca
      601 attttcgtga atttcttctt atttctcgga gttaaacact tctgtcccag cctcttctgt
      661 ccaaattatt ctaagatttc tctttaccaa catcatttga caaagagctt cttttttgaa
      721 tactatattc gttctgtttt tcatcttcaa atacgaactc atccgcagaa atatttgtca
      781 caactccatg aatcttcttt tcattaccac ctatatacca caggattcct acacagaaac
      841 aacaaaaaaa tagcataaaa taggttttct ttattttata tccctccttt tagattaaaa
      901 gctcacagaa aacgctttct aaaaagacaa tagaataatc ataatttttc attttgtatt
      961 atttatacaa ataatacgca tgttact
//
LOCUS       DAYYAG010000272          985 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000272 DAYYAG010000000
VERSION     DAYYAG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.278"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>985
                     /locus_tag="ACHARQ_003039"
     CDS             <1..>985
                     /locus_tag="ACHARQ_003039"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8411369.1"
                     /translation="EPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITDS
                     EYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQLD
                     DTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTGK
                     KNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVGS
                     IAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFYE
                     NFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          262 t
ORIGIN      
        1 tagaaccagg cgattttatc atgagtggcg ctggaacaat tgggagaatt tcaagagtac
       61 caaagcaaat aaaaccgggt gtattcaatc aggctttgat tagacttaga ataaataaag
      121 aaatcacaga ttcagaatat tttattcaat ttattcgtgc agattttatg caaagaaaac
      181 tcacaggagc taatccaggt tcagcaatta caaatctcgt accaatgtca gaagtgaaaa
      241 aatggatagt acaatttcca atattagaag aacaaaagaa aattgggaac ttcttcaaac
      301 aactggacga cactatcgct cttcaacaac gtaagctaga tttattaaag gaaacgaaaa
      361 aaggattctt acaaaaaatg ttccctaaaa acggagcaaa agtgccagaa attcggtttc
      421 ctggatttac ggaagattgg gaacagcgta agttcaaaga gtttagtaag aagactggaa
      481 aaaagaacac gaaagattta gacttccctg cctattcggt aagtaataaa gcaggtttaa
      541 tttctcaaac agaacagttt gatggtagta ggttggatga tttagaaaaa acaaattata
      601 aattcgttga accaaatgaa ttcgcctata atccagcaag agtaaacgta ggatcaattg
      661 catttaacaa tcttggtatg acggtcattg ttagctctct ttatgtagtt gtgaaaatga
      721 gcgaggactt agataatgag ttcatacttc agtttatcaa gtcaccaaca tttatcaaag
      781 aggtcaagcg caacactgaa ggaagcgtac gagaatactt attctatgag aatttcgcta
      841 atatcaagtt tccttttact aggaataaag aggaacaaca aaaaatcggc gctttcttca
      901 aacaactcga cgacacgatc gctcttcatc aacgtaagtt agatttgctg aaggaaacga
      961 aaaaaggctt cttacaaaag atgtt
//
LOCUS       DAYYAG010000273          983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000273 DAYYAG010000000
VERSION     DAYYAG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.279"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..983
                     /locus_tag="ACHARQ_003040"
                     /pseudo
     CDS             370..983
                     /locus_tag="ACHARQ_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          337 a          178 c          183 g          285 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa gga
//
LOCUS       DAYYAG010000274          951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000274 DAYYAG010000000
VERSION     DAYYAG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.280"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..>951
                     /locus_tag="ACHARQ_003041"
     CDS             182..>951
                     /locus_tag="ACHARQ_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFM8411370.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHFFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          317 a          187 c          182 g          265 t
ORIGIN      
        1 actagggcaa tttacgattt aaaagtaaat aaaacattaa taagataaca attaatcgat
       61 ttcattttta tttccttaat tttcatagag agtgtaaaat attttgtgta aatagaaaaa
      121 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
      181 tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga
      241 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
      301 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg
      361 ctcctatgac cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga
      421 ccgcaacggc gagttcaagc aacagactgt tcctgcttat agacggacga atgatacctt
      481 agaggagacc gtcattcact tcttccgaaa aggtattacc atgtcggaaa tcgcagactt
      541 gattgagaaa atgtatgggc atcactacac gccccaaacc atgtccaaca tgactaaagt
      601 gctgactgaa gaagtaaatg catttaaagt tagaactcta aatgataagt atgtcgctat
      661 tttcatggat gctacttata ttccattaaa acggcaaacc gtctccaaag aagcaatcta
      721 tattgccatt ggtatacgag aagacggcac taaagaagta ctaagttatg cgattgctcc
      781 gactgaatcg acctacgttt ggaatgagtt gttacaggac atttactcca gaggggttca
      841 tgatgtctta ctgtttatta ccgatggctt aaaaggcatg aaagatacga ttcaccagat
      901 ttatcctaaa gctaaatacc aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYAG010000275          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000275 DAYYAG010000000
VERSION     DAYYAG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.281"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHARQ_003042"
     rRNA            <1..>943
                     /locus_tag="ACHARQ_003042"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYAG010000276          942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000276 DAYYAG010000000
VERSION     DAYYAG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.282"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>942
                     /locus_tag="ACHARQ_003043"
     CDS             <1..>942
                     /locus_tag="ACHARQ_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411371.1"
                     /translation="TAFHINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLF
                     LLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEI
                     FLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMI
                     IIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQ
                     KRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISIL
                     LCTKIFPWFALQNTPLNIIQAPWRFMIFSTLFISFSV"
BASE COUNT          296 a          136 c          128 g          382 t
ORIGIN      
        1 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
       61 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      121 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      181 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      241 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      301 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      361 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      421 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      481 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      541 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      601 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      661 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      721 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
      781 taggaatctt atctaaaatc acttatataa tgggtataat atctattcta ctttgtacaa
      841 aaatatttcc atggtttgcc ttacaaaata ctcctttaaa tattattcaa gctccttggc
      901 gttttatgat attttcaaca ttgttcatat ctttttcagt at
//
LOCUS       DAYYAG010000277          916 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000277 DAYYAG010000000
VERSION     DAYYAG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.283"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHARQ_003044"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHARQ_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8411372.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          265 a          190 c          138 g          323 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata tagattatct
      901 tttgctttaa aaaaaa
//
LOCUS       DAYYAG010000278          912 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000278 DAYYAG010000000
VERSION     DAYYAG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.284"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..380
                     /locus_tag="ACHARQ_003045"
     CDS             <1..380
                     /locus_tag="ACHARQ_003045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411373.1"
                     /translation="LLSLNRGIIDESDLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVC
                     ETREQVIKEIKSLSEDMVKYQKVFNCYKKYTQESYVMPLNIQTISKNRIRRINKFDPS
                     GNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(705..851)
                     /locus_tag="ACHARQ_003046"
                     /pseudo
     CDS             complement(705..851)
                     /locus_tag="ACHARQ_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          303 a          143 c          180 g          286 t
ORIGIN      
        1 ttttactttc attaaataga ggcatcattg atgaaagcga tttgaatagt tttattaaag
       61 atattgaagc taaaggtgaa aagctttcag tggaagaagt agaaaaacga gctgtaaaac
      121 ataatttggt ttgcgagaca agggaacaag taattaaaga aataaaatca ttatctgaag
      181 atatggtaaa atatcagaag gtgtttaatt gctacaagaa atatacacaa gaatcctatg
      241 taatgccact taatatccaa acgattagta aaaatagaat tagaagaatt aacaagtttg
      301 atccttctgg aaatatgaag gcacctggaa atgtcctcaa tgaagtagat gaaagtttaa
      361 aaaaatattt tacaaagtaa acgatatttt tgttgacatt tttctgttcg ttagttacca
      421 tgtagttata gaaggacgta agcgcctatt tttaatgtac acatcgggac gtatgcgccc
      481 acatagaaac atagttgaga gacatctata tggtgtctct ttttttatta atatgcaaaa
      541 aagtctagtt ttttcaataa tatttatgta ataataaatt agccaccaca acaaagaatg
      601 aaatccatta ttatctagtc tagtccattc tttttgtttg cagtatttgt gatggctttc
      661 cgtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc gtacttctcg
      721 taatccagga aggccgtcag ctccgtccgc agtagcgtgt ttacggccag ctccagatgg
      781 gagcggaata actcgttcaa atcacctttg gtggctagag tctgcaggag ttctgtaata
      841 aaatcgttca tagggaagtc ctcttttctg tgaatgggtg tggtaacttt attctacaga
      901 agggacttcc tt
//
LOCUS       DAYYAG010000279          906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000279 DAYYAG010000000
VERSION     DAYYAG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.285"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..767
                     /locus_tag="ACHARQ_003047"
     CDS             <1..767
                     /locus_tag="ACHARQ_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8411374.1"
                     /translation="IKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLLCKEE
                     IIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKN
                     KGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGML
                     RNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLE
                     IREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          335 a          144 c          164 g          263 t
ORIGIN      
        1 tcatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag
       61 ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt
      121 tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt attgacttag
      181 gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt
      241 ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg
      301 ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag
      361 tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta
      421 cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt
      481 tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta
      541 aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc
      601 cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc
      661 gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca
      721 atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa
      781 gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa
      841 tacgctatca agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca
      901 ggttta
//
LOCUS       DAYYAG010000280          894 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000280 DAYYAG010000000
VERSION     DAYYAG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.286"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            244..630
                     /locus_tag="ACHARQ_003048"
     CDS             244..630
                     /locus_tag="ACHARQ_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8411375.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            724..>894
                     /locus_tag="ACHARQ_003049"
     CDS             724..>894
                     /locus_tag="ACHARQ_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411376.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAIS"
BASE COUNT          324 a          138 c          140 g          292 t
ORIGIN      
        1 aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat gaattttctt
       61 ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga aaataaacat
      121 cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag cataattgca
      181 ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga ggaggtgtgc
      241 aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt tgaaaaggtc
      301 attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa acatgaacaa
      361 cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa acaaccgatt
      421 aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat ccttcctttg
      481 ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa aactgataaa
      541 gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa acatcgactt
      601 tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt ttgtatgctt
      661 ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga agaaggtaga
      721 tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga agaagctgtt
      781 gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag aactggaaaa
      841 tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat ctcg
//
LOCUS       DAYYAG010000281          893 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000281 DAYYAG010000000
VERSION     DAYYAG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.287"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..226)
                     /locus_tag="ACHARQ_003050"
     CDS             complement(<1..226)
                     /locus_tag="ACHARQ_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8411377.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEE"
     gene            complement(248..850)
                     /locus_tag="ACHARQ_003051"
     CDS             complement(248..850)
                     /locus_tag="ACHARQ_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8411378.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          289 a          206 c          132 g          266 t
ORIGIN      
        1 tctcttcata agcagtgtaa ccgacagtct tttcttcgct ttttaataaa aagtaggaca
       61 caccttctgt cagttctttt tgaatctgtt tttttgactg ataagttttc agcatgtaat
      121 ccacttgttt ttgaccaatg atcggcacaa aaacttccgg ccatacttct tgtatgactg
      181 ccatcagctc atccagttca ttttctgtta cttttatttt ttccatacac ccacgtcctt
      241 ttttacatta taaatgacct tgcgcttgat ggaataaaaa gagcatgatc atgctataca
      301 cacccaccga ccacggtttc aagaaaatca caatctgcat aaattcccta aaagataatt
      361 tcgtcaaacc agctactaga cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa
      421 caactagtac ttttttaata tctttcgttt ttcttgtcag caattcatca aatttctgat
      481 acttttttgg cgataaaata agctggacaa aggctacgcc atagtaacgg acaagcaaaa
      541 atccgattgc ttccccaatc actaggccaa tacaactata taaaagccca aatccattgc
      601 caaacatcag catgcccgct actgaggaga tccctcccgg caaaatagga atgatcactt
      661 ggatcacttg caacagaaca aatatcatca ctgcatactt gccaaattgc tggataaacg
      721 cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata agcataaaga atcaacacaa
      781 agaaaaaaaa cagaccaata atgggcatga tctgaagaca tttttgtaaa actgatcctt
      841 ttttagacat ctatattccc cacatctttt ttcatttatt aaaactgcct cct
//
LOCUS       DAYYAG010000282          891 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000282 DAYYAG010000000
VERSION     DAYYAG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.288"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>891
                     /gene="bsh"
                     /locus_tag="ACHARQ_003052"
     CDS             6..>891
                     /gene="bsh"
                     /locus_tag="ACHARQ_003052"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8411379.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQIT"
BASE COUNT          304 a          122 c          161 g          304 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact g
//
LOCUS       DAYYAG010000283          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000283 DAYYAG010000000
VERSION     DAYYAG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.289"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACHARQ_003053"
     CDS             <1..888
                     /locus_tag="ACHARQ_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8411380.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAYYAG010000284          866 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000284 DAYYAG010000000
VERSION     DAYYAG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.291"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..410)
                     /locus_tag="ACHARQ_003054"
     CDS             complement(<1..410)
                     /locus_tag="ACHARQ_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411381.1"
                     /translation="MNVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTF
                     YPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKL
                     SVFSIISLAELIFSLTTPGNALRAAHEINKWFPE"
     gene            complement(480..>866)
                     /locus_tag="ACHARQ_003055"
     CDS             complement(480..>866)
                     /locus_tag="ACHARQ_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8411382.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          281 a          138 c          136 g          311 t
ORIGIN      
        1 tattcaggaa accatttatt aatttcatgt gcagcacgta atgcattccc tggagttgtc
       61 aaagagaaaa ttaattctgc taagcttatt attgaaaata ctgataactt ataattatat
      121 cgtccattga caatcaaata taaactaact atacttgtta gaacaaaaaa acaggcgttg
      181 acttgttctt gattagttaa aaatatcaat aaaggaatac ttacccaata tatttttcta
      241 tttatttctt ctcctcttaa gagttgaaaa aacggataaa aagttagtaa actaaatgct
      301 actggccact gatagttcaa ggtggttgct ctccaaccgg tttcccccat tactgtcaat
      361 ggtattaaac agtatataga gcaaaccaat aataaatttt taacatttat tttctcaaaa
      421 acatactggg agcgtcaata attttgtgta aataaattgt cctcctgcaa aataattagt
      481 tactcagtaa acattgaaac taatgtatcg gttacctgtt gaaaaccttt atggcttctg
      541 tttagaaatt tttgattgta tgtatcaaaa atgctgacta gaaagcgttc tagtgattct
      601 tcattttgaa actgctcttt tctacggctg tatcttttaa tttgcttatt gaaagactcg
      661 attagattgg ttgagtaaat ggttctacga atgctaggtg gaaaatcata aaaagttaat
      721 aagtcttggt tttctatgag tgactgcgtc actttaggat agtttttctt ccatttctca
      781 atcatgccgg ataagaaggt attcgcttct tcttttgagt tagcttgata aacagcctta
      841 aagtcatcac agatttcttt tcggtc
//
LOCUS       DAYYAG010000285          819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000285 DAYYAG010000000
VERSION     DAYYAG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.293"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(353..466)
                     /locus_tag="ACHARQ_003056"
                     /pseudo
     CDS             complement(353..466)
                     /locus_tag="ACHARQ_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          294 a          129 c          130 g          266 t
ORIGIN      
        1 aaacatccat ctggaatttg tctttttcgt tgctttgctc ataaaaatct gcatattcat
       61 cactaatatt ttttctaata aattcttcca tcataaagat cacctcgaaa agagtatacg
      121 aacaaacgtt ctttttgtaa aggtggaatt tattatacta aataaatagg tgaacaagta
      181 cttgtgccaa attatgtgcc aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat
      241 cctattatga taacatttct tataataaca aacacaacag aagacgtgta atagttagtc
      301 aggaacgtac aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag
      361 acattgtagt ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc
      421 cttgataaag taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag
      481 aaaataggga aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt
      541 tcatacttca atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac
      601 gaaagatata tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg
      661 gaatgaactg gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca
      721 actttccttt tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat
      781 ctaagccaat agcttagatc ctttattcta tgctttata
//
LOCUS       DAYYAG010000286          794 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000286 DAYYAG010000000
VERSION     DAYYAG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.294"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            345..>794
                     /locus_tag="ACHARQ_003057"
     CDS             345..>794
                     /locus_tag="ACHARQ_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8411383.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYY"
BASE COUNT          265 a          145 c          126 g          258 t
ORIGIN      
        1 cagaagttct ataagttata atcatagttg ttaggtgatt catctagtaa aaacaccaga
       61 tttcaaattt cacacaattt aaactagatc aatcatcaac cttcttcaaa ttgttagaac
      121 cctttcccga ggagctgcag cataagcaaa aaaacaaaca ggaagccaaa tggctaagac
      181 attttttcta ttagtctagt tgcttctcgt cgcaagttta agactttaga aagttatttc
      241 taatgatgtt tgggttgttt tgatcaatgc ttatgcacag ctccttacct attatttatc
      301 tcaacagatc taacaaatta atggactatt taaggagttt ttttatgctt acgatctatt
      361 cttctcgcac acatagttac catctgatgg caaactggct gaaagaccat caaatgcctt
      421 acagagaacg aattttcaca cctaaacagc ctttaacaag gaaagagata ttttatttat
      481 tgtcattaac agaaaacgga tttgacgatc tattagctaa acgctcgatt gattataaaa
      541 atttgaaaga ccaaatcgac caatgtacga cctcagagct tgtcgatctg atcgtctcgc
      601 attttacttt gttacaacgt cccatcatta ctgacggaaa gaaattatta gtcggattca
      661 atgaagatag catgcgtctt tttatccctg tacaatggcg cagaagtcaa ctgaaatctg
      721 tctataacga ttttacatgg gtaagcaatc tagaaaattc tacggattta gatgatgaat
      781 ttttaaatta ttat
//
LOCUS       DAYYAG010000287          791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000287 DAYYAG010000000
VERSION     DAYYAG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.295"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>791
                     /locus_tag="ACHARQ_003058"
     CDS             <1..>791
                     /locus_tag="ACHARQ_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8411384.1"
                     /translation="KELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVP
                     YIEKVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPT
                     KGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKM
                     KDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGA
                     VVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFN"
BASE COUNT          323 a           85 c          142 g          241 t
ORIGIN      
        1 aaagaattaa ttgatcaagt tctatttgtt aaaaatagaa ctgtaaaaac tacaactatt
       61 cagttgttaa aaactgaaaa caataacgca ttaaggaaat ttgacatttt taatagttcg
      121 attgaagttc cctatatcga aaaggtaagt gcagatgtat taaaagttga agaaattact
      181 tatgaaaata ttctaaaaga cataaatttt gatgtcaaag ttggagatgt tattggtttg
      241 gtagggaaaa acggtgtggg gaaaacaacc ttactaagaa tactcatgaa gattattcgc
      301 cctaccaaag gaaagataat cgaaaataaa aaaaatttgt catcgccgtt tttagtaatg
      361 caagaaatgg attaccaatt tttcacagaa tcagtgagga gcgagttagc cctggggaat
      421 gaaatagtta gtaaagatca gcaagaaaaa attttaaaaa aaatggaact ttataaaatg
      481 aaagatcaat taccgtttga tttatccggt ggagaaaaac aaagattatt ggtttctata
      541 gcttcattgt caaaaactaa tttatttcta tttgatgaac ctacaagtgg gttggattat
      601 attaatatgg aaaggattgc tgaccttatt aaagacttaa aagaaaaggg tgcagtagtt
      661 atagctactc atgatccgga atttctttat aaaacatgca atagaattat ttatttaaaa
      721 aatggtggaa taaaaaaaga tataaaactt agagtagaag attcaacttt gatagaacag
      781 atttttaatg a
//
LOCUS       DAYYAG010000288          750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000288 DAYYAG010000000
VERSION     DAYYAG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.296"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>750
                     /locus_tag="ACHARQ_003059"
     CDS             <1..>750
                     /locus_tag="ACHARQ_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8411385.1"
                     /translation="IEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYST
                     SQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLE
                     SVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIF
                     ELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSY
                     LILISIIVLSVVFASSLMLIKKPKEILSKIS"
BASE COUNT          292 a           69 c          120 g          269 t
ORIGIN      
        1 tattgaaaaa agtggaaaaa taaaaagtca taaatttaaa attataggga tcttttctgg
       61 taaaaaacag gaaacatata caggattatc ttctgatttt agtgaaaata tggtgtttgt
      121 agattattca acaagccaag agatattaaa taattcagaa aataatgaaa ttgcaaataa
      181 aattttaatg tattctggta gtgcagaatc tacagactta gccttaaaca aattgaaaga
      241 gcttaaaatt gatgagtcaa agtattttgt tgaaaaagat tctaatgcgt ttgaagagtc
      301 tttagagtca gtgagtggaa taaaatatat aataaaagta atgacttatt ctattatgtt
      361 aggtgggatg gttgttcttt cattaatctt gattctatgg ttaagggaaa gaatttatga
      421 aataggtata ttcttatcta ttggaacatc taagatacaa attataatgc aatttatatt
      481 cgagttaata ttcatatcga taccaagtat aatatcttcc ttatttttag gaaatgtatt
      541 actaaaagta attgtagatg gattcattaa ctcagaggat tcaatgattt ccggtggaag
      601 tttaataaat aatagtagtt ttatgtcaaa tataacaaca cttggacaaa gttatttaat
      661 attaataagt attattgttt tatcagttgt atttgcttct tcattaatgt taatcaagaa
      721 gcctaaagaa atcttatcaa aaataagtta
//
LOCUS       DAYYAG010000289          701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000289 DAYYAG010000000
VERSION     DAYYAG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.297"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..284
                     /locus_tag="ACHARQ_003060"
     CDS             <1..284
                     /locus_tag="ACHARQ_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8411386.1"
                     /translation="QLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNKNY
                     FKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(320..691)
                     /locus_tag="ACHARQ_003061"
     CDS             complement(320..691)
                     /locus_tag="ACHARQ_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411387.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          225 a          117 c           96 g          263 t
ORIGIN      
        1 cacaattatg gtttaaattt gacccttcga atagatttat caaagatttt tataaagtat
       61 ggaattcaga agttttcttt ttagcaatcg aagatagctt attaatcaat ctctactatt
      121 ctaataagaa ctatttcaaa attcctgctg cgaaaactag aatgaagaag ggtgtatact
      181 ttttgtttga tatcgtgact gacgtgccgg acgcacgtag cgatcatcgg cgttatgact
      241 atataaagta tactttcgtt gatccagaaa gatacaaaga ttaaataaaa aaataacaaa
      301 caaactcgct tgaaattcat taggtgagtt tgtttattat tttttctgct tctcgattca
      361 tcagcgccat atctgttttc gtaatggttt cattaggaat atagtcagca cattttcttt
      421 tctctaacat tctataccca ctattatgta ttcctaaggt attcaattcc ttaactgttg
      481 aaaacaaatt ccaaacggtt tggtggcttc cttttccgct acttccatta taaataatat
      541 cataattttc tttcaaatac tcatcagatt ttttaaaagc actgtagtat gctcttgaaa
      601 cagcagttct tctataagat tctttagaat tatatttatc atctgattca atttgtttgg
      661 aaatagataa atattcatta aagtcaaaca ttttattccc c
//
LOCUS       DAYYAG010000290          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000290 DAYYAG010000000
VERSION     DAYYAG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.298"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHARQ_003062"
     CDS             complement(54..629)
                     /locus_tag="ACHARQ_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8411388.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYAG010000291          677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000291 DAYYAG010000000
VERSION     DAYYAG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.299"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>677
                     /locus_tag="ACHARQ_003063"
     CDS             <1..>677
                     /locus_tag="ACHARQ_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8411389.1"
                     /translation="FFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAV"
BASE COUNT          270 a           82 c          122 g          203 t
ORIGIN      
        1 tttttcaaag atacttggaa aaatattaaa gatacggcat acaatacgtg gatttctata
       61 aaaaatacca tgattaatac atggaataac attaaggact ctttctggaa tattgttact
      121 ggaattgtta attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct
      181 attaatcggg taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc
      241 aagaacatca ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat
      301 actatcaaag gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat
      361 tctccatcaa gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt
      421 ggtattgata aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg
      481 tctgttcaac ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat
      541 atatctgcta gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct
      601 gattcaatgg ttcgaaagat tactacaaaa attaaaacag aattgacaag acaaatgaaa
      661 cgagatgctg gtgcagt
//
LOCUS       DAYYAG010000292          674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000292 DAYYAG010000000
VERSION     DAYYAG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.300"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..481)
                     /locus_tag="ACHARQ_003064"
     CDS             complement(83..481)
                     /locus_tag="ACHARQ_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM8411390.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          216 a          138 c           85 g          235 t
ORIGIN      
        1 atattctttg ataatttgtc tttcttaaat ccatacgtcc taaaacaagc tgtttttgct
       61 taatatgatc atctgagaga catcaaatca acttttctct caacaaatca ttcaaagctt
      121 tcgacagatt aatattctct tcagccgcta aaacatttaa atagtgttcc acagtcactg
      181 attttcgatc ataattttgc ttgtttcgag ctttagcaac tttcgtatta gcttttacga
      241 caaataaatt aacattctca gatcgcttga ttcttgcatt taactcttga acaggcgtag
      301 gcgatggaat ttctttttct tgcaactcta aaagcaccaa gtatccttcc aatacttctt
      361 gcgccatctc aaaagcatct tctaaagttt caccatgtgt atataagtct ggaaaatctg
      421 ggaatttaac tgcatattca tttcctttcc attcgaatac tgcacaaaaa aagtatttca
      481 tcgcattgca cctctctaat taaaaaataa tttttgcttt tttgtttcaa aaaaaagaat
      541 cgcctaccaa aaaaattgat aggcgatcac aagctaatta acctctaaag gagattaata
      601 ccttgctccc aaggcactat atatcttagc atacgtctga agcatcttca aacgagtaag
      661 atatattttg catt
//
LOCUS       DAYYAG010000293          670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000293 DAYYAG010000000
VERSION     DAYYAG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.301"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..104)
                     /locus_tag="ACHARQ_003065"
     CDS             complement(<1..104)
                     /locus_tag="ACHARQ_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8411391.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITA"
     gene            complement(104..>670)
                     /locus_tag="ACHARQ_003066"
     CDS             complement(104..>670)
                     /locus_tag="ACHARQ_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8411392.1"
                     /translation="ISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWI
                     IDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTII
                     GEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFT
                     LLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          228 a          120 c           76 g          246 t
ORIGIN      
        1 gctgttatat caaaattaat agaattcttt tttccatagc ttattagatt ttttagatgt
       61 ctctttccaa ttgaccctaa cccaaaaaaa attactttca tcattaattt acctcaatgt
      121 cgttaattaa gttaataata tacttatctt tttcaaaagt atattggggt tccattttct
      181 tttccaaaag cgtgaaaaat tctttaatct cctctaaata tgcatcttca ataatagtat
      241 gtgaataatt attatcatgc ataacctcat cgtacaattg aatatctgtc atactagaag
      301 tttcttcatt ccatgaaaat agagaatccg ggttaccttc ccattctatt aaaatctttt
      361 caccaattat agttaaattt cttttagcta ctcttgaaac aacatctaca cttaaagttc
      421 caactatacc agtttcatgc tcaataataa gagtgaaact gtccgaataa tcaatatcaa
      481 gattggataa tttctgggtc attacagtaa acgatttaac tttcccaaat gtatctataa
      541 tccaaggtaa ttcaatagcg aatatttctc tacacccatt agtttttttg tttgaaacaa
      601 aaaaattgcg ataatcttcc caaggatgcc agtcgggtaa atactgtcct acatggtatt
      661 gataagaaat
//
LOCUS       DAYYAG010000294          669 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000294 DAYYAG010000000
VERSION     DAYYAG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.302"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>669
                     /locus_tag="ACHARQ_003067"
     CDS             <1..>669
                     /locus_tag="ACHARQ_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411393.1"
                     /translation="KQLRLSCGFTMEELGRKIDNSPRATISNWERGTNLPNPQKLKLL
                     STITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKDIQERYSDTFSLD
                     KDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRHSISKELFFKRAN
                     SKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVEDFFYKITKNQFET
                     EKFIS"
BASE COUNT          271 a          103 c           98 g          197 t
ORIGIN      
        1 aaacaattac gcctttcatg tggttttact atggaagaac ttggaaggaa aatagataat
       61 tcaccacgtg cgaccatatc caactgggaa cgtgggacca atttaccaaa tccacaaaag
      121 ctgaaattat tatcaactat cactaattca acaattgact ggattaaatg gggaacttta
      181 gaagaatata taacttcata ccttatcgat atagggtatg agctatatat taaggacttc
      241 ccagaaatac ctcacaaagt ctttaaagac attcaagaaa gatatagtga tacgttttcg
      301 ctagataaag actatgagtt attgaatcct ataataaaaa atatatttac aaaatattac
      361 tcaaaagatt ttgaaattta tctaaatgag atacttgcta ataatatcaa tcctctgatt
      421 gaagagtacg ctaaccaaag acactctatc tctaaagagc tatttttcaa aagagccaac
      481 agtaaacttc aagacaagat aagaaatggt gatttcaaat acggtgaaaa agaaagaatg
      541 attgattctt taaaacagct attagacgaa atggacttag cctataaaga tatttcaaag
      601 tattcatctg tcgaggattt cttttataaa attactaaaa accaatttga aacagaaaaa
      661 tttatttca
//
LOCUS       DAYYAG010000295          642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000295 DAYYAG010000000
VERSION     DAYYAG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.303"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..541
                     /locus_tag="ACHARQ_003068"
     CDS             95..541
                     /locus_tag="ACHARQ_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8411394.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          221 a           91 c          125 g          205 t
ORIGIN      
        1 aatacaataa aattgaattt tcttttcatg ttaagtggat tttaaaatgt aaaaatagta
       61 tgataagacg agaataacaa cgtggggtgt ttacatggaa aataagttag tcgaagaggt
      121 tatcgaaaag ttaaaaaaag agaacattcg aatcacacct caacggtacg cggttttaga
      181 atatttgatt gaacatcatt cccatccaac ggctgatgag atttataaag cacttgaaca
      241 tcggtttcca aatatgagtg ttgcgactgt ttacaataac cttcgcttat ttacttcgat
      301 tggatttgtt caagaaatga agtacggcga tgcttctagc cgatttgatt tcagttcaaa
      361 gcgtcattat catgtaattt gtgaaaactg cggtaagatt gttgattttc attacccagg
      421 gcttgaagat atagaaatgg ctgcaagcag actgactgat tttgagatta atgaacatcg
      481 gttggaattg tatggactct gtccggattg taaagagtca gcaaaaaagg cgcaaatgta
      541 aaaacagttt ctacaagtgt tatacctaaa aaaagctagc tgtgtaaaaa aaacgctagc
      601 ttttttattt ttttgtcttt tattcacaat cttagatata aa
//
LOCUS       DAYYAG010000296          640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000296 DAYYAG010000000
VERSION     DAYYAG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.304"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..294
                     /locus_tag="ACHARQ_003069"
     CDS             <1..294
                     /locus_tag="ACHARQ_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411395.1"
                     /translation="PTALSFSVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSV
                     SLPPQVNDINFNINARNVLVEEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            308..>640
                     /locus_tag="ACHARQ_003070"
     CDS             308..>640
                     /locus_tag="ACHARQ_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411396.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLD"
BASE COUNT          235 a           86 c          120 g          199 t
ORIGIN      
        1 cctactgctt tatcattttc tgtaacagct gtggtgtctg agatagagga tacagaaaag
       61 ataaaaatag tagaaattga agttttaaac aagaatgaaa aacaaatttt ttctacgggt
      121 gaagtatcgg taagcttgcc tcctcaagtt aacgatataa actttaatat aaacgccaga
      181 aatgtcttgg ttgaagaagc aggagagcat tatgctgttg ttaaatttaa tggaactgag
      241 attggaaggc aaatctttga tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac
      301 gatacggatg cttgatatag taccatcaaa tacacatcgg agtggtagct taataaattt
      361 tgctgcgatt tttgcttgcg catcggtaat tctagcatct ccagttgttg tggatgcaag
      421 ttctactccg aaaactaaaa atgataatca acttgtaatt actacaaaaa atgagattaa
      481 cacaatttca agtaatgatg gcaaaatttt tgatgtgata aatacggttt taaaaagaaa
      541 agctgaaatc aatataaaat atgatgaaga tttaaacttg tatttctttg ttatcaaaac
      601 aacatctgaa ttgttttcct cagattacga tgttttggat
//
LOCUS       DAYYAG010000297          618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000297 DAYYAG010000000
VERSION     DAYYAG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.305"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..535)
                     /locus_tag="ACHARQ_003071"
     CDS             complement(170..535)
                     /locus_tag="ACHARQ_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411397.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          238 a           82 c          114 g          184 t
ORIGIN      
        1 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
       61 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata
      121 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      181 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      241 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      301 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      361 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      421 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      481 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
      541 atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata
      601 atcaattact ttttcatt
//
LOCUS       DAYYAG010000298          613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000298 DAYYAG010000000
VERSION     DAYYAG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.306"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>613
                     /locus_tag="ACHARQ_003072"
     rRNA            <1..>613
                     /locus_tag="ACHARQ_003072"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          151 a          137 c          192 g          133 t
ORIGIN      
        1 ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt ggaattccat
       61 gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg
      121 tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta
      181 gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag
      241 ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt
      301 gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct
      361 taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag
      421 tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca
      481 acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag caagactgcc
      541 ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt atgacctggg
      601 ctacacacgt gct
//
LOCUS       DAYYAG010000299          565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000299 DAYYAG010000000
VERSION     DAYYAG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.308"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..>565
                     /locus_tag="ACHARQ_003073"
     CDS             69..>565
                     /locus_tag="ACHARQ_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8411398.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          210 a          104 c           93 g          158 t
ORIGIN      
        1 caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg
       61 acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt
      121 taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat
      181 taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga
      241 gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac
      301 ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg
      361 atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg
      421 ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga
      481 ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg
      541 tcgctatttt tatggacgct actta
//
LOCUS       DAYYAG010000300          564 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000300 DAYYAG010000000
VERSION     DAYYAG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.309"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..449
                     /locus_tag="ACHARQ_003074"
     CDS             81..449
                     /locus_tag="ACHARQ_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8411399.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          201 a           96 c           89 g          178 t
ORIGIN      
        1 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
       61 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcag atcaaagaaa
      121 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
      181 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
      241 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
      301 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
      361 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
      421 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
      481 ggaaactatt catcagctta tacaggtttt gttcaatcct atccttttta tcatccctgg
      541 ttaaacactt aattgacaaa gtat
//
LOCUS       DAYYAG010000301          552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000301 DAYYAG010000000
VERSION     DAYYAG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.311"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..>552
                     /locus_tag="ACHARQ_003075"
     CDS             142..>552
                     /locus_tag="ACHARQ_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8411400.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEIN"
BASE COUNT          247 a           47 c           97 g          161 t
ORIGIN      
        1 gtttgtatgc agttacatct gactttattc gaattaagga aaagatttat aaaaataaaa
       61 taaaaaaata tatatttaaa ttgacttatt gtgaaatcat taactataat tagttcagtt
      121 tatgttaagg agaatgatga tatgaataag aaaattgtag taagtggatt aattataggt
      181 ggattagtgt tagcagggat cgcagggaaa aactttgccg atgacatttt gttatggggt
      241 ggagaagata atattgaaca aatcaatcat aatttggagt cattagataa agcactagaa
      301 aataaagagc aaaatattag tagtttaaat tcacgattat cttcaaataa tcaagaactt
      361 gagcaagcaa aggctaacgt tgaagaatat aaacaaaaag tgttgacttt ggaaaatgat
      421 aaaaatcaat taactataga gaaaacaaat ttagagaatc aattatcagg aaaaaatgct
      481 gaattgcaag gtaaacaaaa tgaaatcaat gctaaaattg atgaaataaa tcaaaaaata
      541 gctgaaataa at
//
LOCUS       DAYYAG010000302          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000302 DAYYAG010000000
VERSION     DAYYAG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.312"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHARQ_003076"
     CDS             <1..>534
                     /locus_tag="ACHARQ_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8411401.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYAG010000303          529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000303 DAYYAG010000000
VERSION     DAYYAG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.313"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..524
                     /locus_tag="ACHARQ_003077"
     CDS             <1..524
                     /locus_tag="ACHARQ_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411402.1"
                     /translation="GYFSMWDTFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLA
                     VVSFCFSMTVGVMWEFVEFSADKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITR
                     TIIESQNADGKTDKTIIQNGYLDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKF
                     TFIKNFIPKKIKR"
BASE COUNT          171 a           82 c           98 g          178 t
ORIGIN      
        1 atggctattt ttcaatgtgg gatacttttc tacatgcatt cagcggattt ttagctgggg
       61 gtgtcggctt ttcccttgtt tatttgctca ataaaaatac aaaaaccatg aacttgactc
      121 ctttattact agcagttgtt tccttctgtt tttcgatgac agttggtgtt atgtgggagt
      181 ttgtggaatt ttcagcagat aaattactgc aattcgatat gcaaaaagat acgatcatcc
      241 agcaaatcaa tagtgtggag ttaagtaaag acggaaacac tgtctatcat gttccagata
      301 tcactcggac aatcattgaa agtcaaaatg cagatggtaa aacagataaa acaatcatcc
      361 aaaatggtta cttagacgtg ggaatcatgg atactatgaa ggatttattt gttaatttta
      421 ttggagcagt tgttttcagt gtatttggtt acctctatag tcgaaacgat taccaaaaat
      481 ttacgttcat caagaatttt attcctaaaa aaatcaaacg ataaaacat
//
LOCUS       DAYYAG010000304          527 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000304 DAYYAG010000000
VERSION     DAYYAG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.314"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..>527
                     /locus_tag="ACHARQ_003078"
     CDS             59..>527
                     /locus_tag="ACHARQ_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8411403.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAI"
BASE COUNT          189 a           81 c          111 g          146 t
ORIGIN      
        1 atttatattg cgagtagaaa aaaaatatgc tattatttgt attgaggtga tcgtgaaaat
       61 gcttacagag gaacgtagac aaaaaatcct agacttgctt gaacagcaga aaatcgtgaa
      121 atcacaagat cttgtaaacc tttttaatgc ttctgaatca acgataagac gtgatttaca
      181 agagctggag aatagtgggt ttctagaaag agttcatggc ggagctaaga aagagcaatt
      241 attgaatttt gagcagaata tgagtgaaaa atcactcaaa aacgttcacg aaaaacagca
      301 aatcgccaaa tcggcagctg aaaccattaa tgacgaagat gtcatttatt tagacgctgg
      361 ctctactacg ttagaaatga ttccttttct gcaaggaaaa cagatcacag tagtgacaaa
      421 ttccgttcaa catgcggcaa atttagttga tttggagctt gctaccatta ttttaggtgg
      481 aacgataaag ctggcaacta atgctgtttt aggatctaat gcgatag
//
LOCUS       DAYYAG010000305          518 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000305 DAYYAG010000000
VERSION     DAYYAG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.315"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACHARQ_003079"
     CDS             <1..159
                     /locus_tag="ACHARQ_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8411404.1"
                     /translation="SFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANE
                     VLKIAEEK"
     gene            160..>518
                     /locus_tag="ACHARQ_003080"
     CDS             160..>518
                     /locus_tag="ACHARQ_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8411405.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREV"
BASE COUNT          197 a           60 c          103 g          158 t
ORIGIN      
        1 agttttgaag aaaacattgt taaagaaatg cctgaatctg ctaatgatat gtatgagaag
       61 gagttgaaat atttctttga aaacgtagta acaggcaaag aaaactggaa tgatttatat
      121 catgcaaacg aagtattgaa aatagctgag gagaaataaa tggaaatttt atttactatt
      181 tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag
      241 cccctagtcc atttcgcttt agcaatgata gatatctatc aacaaaaaca tcctgaacat
      301 aattgcacga cttgtttgaa tacggacagt aatgagttag tagaattgac gactaagtta
      361 aatccgtctg tgaaaattgt agaacgtaaa cctgaattag ctacagatac tgtagggaaa
      421 attgaagtga tacgtgattc attcttacaa ctagaaagag aagataatat atttgattta
      481 attatagatt tagatttgac ttcaccttta cgtgaagt
//
LOCUS       DAYYAG010000306          504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000306 DAYYAG010000000
VERSION     DAYYAG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.316"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>504
                     /locus_tag="ACHARQ_003081"
     CDS             <1..>504
                     /locus_tag="ACHARQ_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8411406.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKL
                     ALKGITI"
BASE COUNT          166 a           94 c           89 g          155 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaatc catgaaaagc
      481 ttgcccttaa gggcattact atat
//
LOCUS       DAYYAG010000307          504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000307 DAYYAG010000000
VERSION     DAYYAG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.317"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..>504)
                     /locus_tag="ACHARQ_003082"
     CDS             complement(206..>504)
                     /locus_tag="ACHARQ_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8411407.1"
                     /translation="LFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLYY
                     SNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          180 a           83 c           70 g          171 t
ORIGIN      
        1 taccatattt ttgaaaaaaa gtgagaaaaa aggcttacaa ttggagtggt agttaattag
       61 tgacttattt ttgattttat agcactgata ctataaaata tagatatcat catattacac
      121 aatcttaata ctaacttaaa aaatatctcc tttcataagt atggtgataa aatccgttcc
      181 gggctacctt tttaggtagc ctactttaat ctttgtatct ttctggatca acgaaagtat
      241 actttatata gtcataacgc cgatgatcgc ttcgagcgtc tagcacatca gtcacgatat
      301 caaacaaaaa atatacgtct ttcttcattc tagttttcgc agcagggatt ttaaagtagt
      361 tcttattaga atagtagaga ttaattaata agctatcttc gattgctaaa aagaaaactt
      421 ctgaatccca caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc
      481 ataattgcgt ctcattaaaa agca
//
LOCUS       DAYYAG010000308          470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000308 DAYYAG010000000
VERSION     DAYYAG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.318"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..114)
                     /locus_tag="ACHARQ_003083"
     CDS             complement(<1..114)
                     /locus_tag="ACHARQ_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8411408.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIE"
BASE COUNT          167 a           87 c           86 g          130 t
ORIGIN      
        1 ttcgatgtca ttggcaacag agatttcaga taagatagac aattgattgt cgtctgttaa
       61 aaaacaaaca tctagttttt cagagctaac gtcaataccg acaaataatt tcatggaaga
      121 aggacctcct ttcgatataa attttggaaa atgcttcgat actgtgggat ttcccagaaa
      181 ctcactgtat agccacaacc tcgcctaata gaatacggtg ataaaagagc tagaagctgc
      241 cttccaaact actatttgaa agtctagtcc attatcgaaa cagctaatga gttggtagtg
      301 ataacagtgg ttcgggtaac agactaaaca gggtagacaa acgctacagg aggaaatagc
      361 aaattcccgt tggatccttt ccatacatcc attgtactct gagaaatcac acaatatcga
      421 aacaaattcc gttaggaatg tgttattcaa taaattttat aatctagaaa
//
LOCUS       DAYYAG010000309          465 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000309 DAYYAG010000000
VERSION     DAYYAG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.319"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..380
                     /locus_tag="ACHARQ_003084"
     CDS             45..380
                     /locus_tag="ACHARQ_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411409.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          173 a           59 c           87 g          146 t
ORIGIN      
        1 caatttccta ggaaaaatag ttaacataaa aggaatgaat tgttatgaaa caatatttaa
       61 atgaatggaa agcgatagag gggtcattag taacgcaaag aataaaggaa ttacctgatt
      121 gcttagagaa ggatcatttg tttcaaatac gagaaatgtt gaaaaaagaa cagtttgatc
      181 cagaccaatt tttagtagtc gagtatccaa ctattggagt gtactgttgt aaccatatca
      241 atgatggaaa atattttatt attcaggagt atggaggaca gttaactcct ttctatacta
      301 catgggaaat gagtgaagaa ggaatcaata attttccatg tgaaagcatc gaagattcaa
      361 tttcacacac cgaatgttaa ttgattataa ctataaaaaa ggattgaaca ttatgttcaa
      421 tccttttttc aatgaaatgt tttccgccat ttcattgttt ggtgc
//
LOCUS       DAYYAG010000310          463 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000310 DAYYAG010000000
VERSION     DAYYAG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.321"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>463
                     /locus_tag="ACHARQ_003085"
     CDS             <1..>463
                     /locus_tag="ACHARQ_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8411410.1"
                     /translation="RKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSC
                     GEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCP
                     FDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT          148 a           78 c           92 g          145 t
ORIGIN      
        1 tcgtaaattc ttaagagaca acccattaga agcgatcgta acagacttga cttatgtacg
       61 agtcgggaaa aaatggaatt atgtctgttt cattttggat ctgttcaatc gagaaattct
      121 cggctattct tgtggagaac ataaagatgc cgttctagta aaaaaagcat ttagccgtat
      181 caaacaacct ctgacagagg ttgagatttt tcatactgat cgtggaaaag agtttgataa
      241 ccaagctatt gatgaattat taacaacttt tgacatcaat cgatcattga gtcataaagg
      301 ctgtcctttt gataatgccg tagctgaatc aacttataag tcgttgaaag tagaatttgt
      361 ctatcaatac acatttgaaa ccttacaaca attggatttg gagttatttg actatgtcaa
      421 ttggtggaac caccttcggt tgcacggtac acttggctac gag
//
LOCUS       DAYYAG010000311          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000311 DAYYAG010000000
VERSION     DAYYAG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.322"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..>462
                     /locus_tag="ACHARQ_003086"
     CDS             163..>462
                     /locus_tag="ACHARQ_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8411411.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSV"
BASE COUNT          171 a           74 c           69 g          148 t
ORIGIN      
        1 tggaaaaaat aaaatgtgtt ttactgacat tgagttatgt tataaattta cgatacattt
       61 cttattgtaa cgtatcgaaa acaggtctat acttattcaa tggaatcgta ccagttccaa
      121 gtttttaagc tcgtaccaag cttagaaaga agaggcaatc gtatgaaaat aacagaaaag
      181 aatgtaaacc cgaaatttga aactaccgaa agcaatctaa aaaagtttga tttaactgtt
      241 actttatcga atcaacttgt agatagcatt gttgaaatgg atgctatgcc acaaaaaatt
      301 tatctttacg ctataggaca tcttgaccct aaaaatccgc cttttaataa taccgtcaaa
      361 gtatctaaaa aggctttatt tgcgttgatg tcttctagtt catcttcttc aaaacattca
      421 agattaaaac aagttattga aaaattgcat cagcaatctg tc
//
LOCUS       DAYYAG010000312          455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000312 DAYYAG010000000
VERSION     DAYYAG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.323"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>455
                     /locus_tag="ACHARQ_003087"
     CDS             <1..>455
                     /locus_tag="ACHARQ_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8411412.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          184 a           67 c          106 g           98 t
ORIGIN      
        1 agtattgaaa gtggaacaac agtctttgca gtgaaagacg gcgtaagctt accagaagat
       61 aaacttccag tgttgaaagc aaaagatggt tatacagatg cgaaatggcc agaggaagca
      121 actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat
      181 aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga attgaataag
      241 gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga tactaagtat
      301 acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac tgttgtagtg
      361 acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac agacaatcgt
      421 tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAYYAG010000313          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000313 DAYYAG010000000
VERSION     DAYYAG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.324"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>451)
                     /locus_tag="ACHARQ_003088"
     CDS             complement(37..>451)
                     /locus_tag="ACHARQ_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411413.1"
                     /translation="RKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAY
                     NQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWG
                     NLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          137 a           83 c           75 g          156 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg t
//
LOCUS       DAYYAG010000314          433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000314 DAYYAG010000000
VERSION     DAYYAG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.325"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          132 a           87 c           51 g          163 t
ORIGIN      
        1 tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact
       61 tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc
      121 aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt
      181 agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc
      241 aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa
      301 gacatctcga ttcatattca agataggaat ctttttatca acatcacttt attcgtattt
      361 tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact attcagccca
      421 ttcaacgacc aat
//
LOCUS       DAYYAG010000315          429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000315 DAYYAG010000000
VERSION     DAYYAG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.326"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>429
                     /locus_tag="ACHARQ_003089"
     CDS             <1..>429
                     /locus_tag="ACHARQ_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8411414.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILST"
BASE COUNT          157 a           75 c           88 g          109 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 cttagcaca
//
LOCUS       DAYYAG010000316          424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000316 DAYYAG010000000
VERSION     DAYYAG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.327"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>424
                     /locus_tag="ACHARQ_003090"
     CDS             <1..>424
                     /locus_tag="ACHARQ_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8411415.1"
                     /translation="EVIENQIICHPIIYKSIFKYAFFASNDFQRMEITDCIFENCDFS
                     GCILTKSMLHRVKFVNCKLTGTKLMESYIGNTLFENCKMDYVNLSGSNIKESTFEHSV
                     LSSADFVDCSLTKIMFSTNDLENASFFDTDLKNIDLSSN"
BASE COUNT          147 a           62 c           69 g          146 t
ORIGIN      
        1 agaggtcatc gaaaaccaaa taatctgcca tccaattatt tacaaatcta ttttcaagta
       61 tgcatttttt gcttccaatg attttcaaag aatggagatt accgactgta tattcgaaaa
      121 ctgtgatttt tcaggctgta tattgacaaa aagcatgttg catcgcgtga aatttgtcaa
      181 ttgtaaatta acaggtacta aattgatgga aagttatatt gggaacacgt tattcgagaa
      241 ttgtaaaatg gactatgtaa atttatctgg ttcaaacata aaagaaagta cctttgagca
      301 ctctgtctta tcaagcgcag attttgtaga ctgttccctc actaaaataa tgttttctac
      361 taatgattta gaaaatgcta gcttttttga tactgattta aaaaatatcg atttaagtag
      421 caac
//
LOCUS       DAYYAG010000317          423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000317 DAYYAG010000000
VERSION     DAYYAG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.328"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..387
                     /locus_tag="ACHARQ_003091"
     CDS             88..387
                     /locus_tag="ACHARQ_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8411416.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          165 a           48 c           86 g          124 t
ORIGIN      
        1 agtaatgatt taaaaaaatc tagatgtagc gtatactttg tttatagaat gtagtgggaa
       61 gaaatatatt tttgggagga aatcgtgatg tctaaaagtg aagaaataga aaacatggta
      121 ctaaaattat atgatctcgt tttaaatcca gagatcaaag aaaaggaacg tatacttttg
      181 atagacgcaa agacaggctt agaaaaaggt cagtattatc ctaaagtcat aaataatttg
      241 gaacgtagtt tacgtccgtt agcaatcagg ggagaattgt ctaaaccagt atcaccgttt
      301 tatatggaga tatcaacaat aggaaaattt gaaaaagaat taggtcgagg aatggcttcg
      361 gcaccgatta cttttgggca tttataaaaa cgagttattc atctcaatga attaaaaagt
      421 ata
//
LOCUS       DAYYAG010000318          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000318 DAYYAG010000000
VERSION     DAYYAG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.329"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACHARQ_003092"
     CDS             complement(<1..339)
                     /locus_tag="ACHARQ_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411417.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAYYAG010000319          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000319 DAYYAG010000000
VERSION     DAYYAG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.330"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHARQ_003093"
     CDS             complement(109..>414)
                     /locus_tag="ACHARQ_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8411418.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAYYAG010000320          391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000320 DAYYAG010000000
VERSION     DAYYAG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.331"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          114 a           71 c           52 g          154 t
ORIGIN      
        1 aagaaagttt aaacattgcg caatttaatt attttagctg tttcttttca taaaaaaaca
       61 tgttgtgact ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa
      121 tattacgagg catcatatta atctatcagc actagtacct aaactagcat ccctttcgaa
      181 tattttttct tatataataa agtttgcttt cactcctcta atctcaccaa atcgactggc
      241 ttgcgttgtg tgatactagg aactgattgg tttagaacga cttttctatt gtaatcatat
      301 ttttcgccat cctcgttgtc ttctaatcct tcaatgaatg atccctaatg tgtgtattaa
      361 ataatttaat ttttttgttc ctatttattt a
//
LOCUS       DAYYAG010000321          355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000321 DAYYAG010000000
VERSION     DAYYAG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.334"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>355)
                     /locus_tag="ACHARQ_003094"
     CDS             complement(<1..>355)
                     /locus_tag="ACHARQ_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411419.1"
                     /translation="YTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTT
                     GSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKV
                     STSYTVTTIISYLVTL"
BASE COUNT          106 a           66 c           51 g          132 t
ORIGIN      
        1 taatgtaaca agataagaaa taatagtcgt tactgtataa cttgtgctta ctttaatatc
       61 agcaactcct ttgactccgc ttatttgtac caatccatta gcattcacct gctcctttac
      121 aggttgatga tttatttcta cattcgttcc taaataagca tatacaggaa gtgtgattac
      181 ttggctcttc tcattagtta ctctatatat gatagaacta ccagttgttt tataaggaag
      241 gtttacccat tgactgccaa tctttgcttc gtgttttatt atatgctcct tattctccaa
      301 tgaagcgata ggagaataat catataaaaa ataatccatt cctttagtgg tatac
//
LOCUS       DAYYAG010000322          346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000322 DAYYAG010000000
VERSION     DAYYAG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.336"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           65 c           57 g          111 t
ORIGIN      
        1 gatctgttct aacattctca actttgtcag tttaccatag gaaaatggga aaatctacga
       61 aagctctcaa cagaatcgtc ctacttaaat acgcttttct ttaataataa ataagttggt
      121 tgtttttgtt atttgcgctt tcacttttcg gaataaataa tgtgtattta tatggaataa
      181 ataaaaaaag aggtcgtgac taaaaacaag tcacaacctc tggtaaacgg tgttctcctg
      241 acaactcctc ggtcacaaga cacaatattc aacaaacatg agaagctatt ttttgaaatt
      301 gctctttgtg actcagagtg aaacggcagg ctcacgacct ctttct
//
LOCUS       DAYYAG010000323          315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000323 DAYYAG010000000
VERSION     DAYYAG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.339"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHARQ_003095"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHARQ_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8411420.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           99 a           48 c           50 g          118 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactc
//
LOCUS       DAYYAG010000324          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000324 DAYYAG010000000
VERSION     DAYYAG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.340"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHARQ_003096"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHARQ_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYAG010000325          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000325 DAYYAG010000000
VERSION     DAYYAG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.341"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..230
                     /locus_tag="ACHARQ_003097"
     CDS             <1..230
                     /locus_tag="ACHARQ_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFM8411421.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT          119 a           56 c           56 g           78 t
ORIGIN      
        1 tgattgatag agattacttt actcaggcag acttgatcat tacactttgt ggtgatgcca
       61 aagacaagtg ccctgccgtt ccaaacaatc acgaacacct tcactgggac ctaaaagatc
      121 ctgcacaagc tgtcgggagc gaagaagaaa tattaacgga attcagaaaa gtcagagaag
      181 gaatcaaaag aaaagtttta gacttaaatc aacgtacatc gatcgtataa agaagtattc
      241 aaaatgattt ttcaagcact aagaaacaat gttaaaaaaa tagctttcca tattttacaa
      301 aattgtgcc
//
LOCUS       DAYYAG010000326          305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000326 DAYYAG010000000
VERSION     DAYYAG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.342"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>305
                     /locus_tag="ACHARQ_003098"
     CDS             <1..>305
                     /locus_tag="ACHARQ_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8411422.1"
                     /translation="RFKEITGIASEKYQKTFPNRQLAIKAENDMKKKIEKVLREENAN
                     SLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITISNTKDIFRLHILPMFGEY"
BASE COUNT          135 a           36 c           53 g           81 t
ORIGIN      
        1 aaaggtttaa agaaataaca ggaattgcat cagaaaagta tcaaaaaaca tttccaaata
       61 ggcaattggc gattaaagcc gaaaatgata tgaagaagaa aatagaaaaa gttttgagag
      121 aggagaatgc aaactcatta gaattaaaag gaaaaataac gtttaaaaaa ttttacgaat
      181 ctaaatggct tcctagatat gaattaggtc aaacaattag atcaaacagg cctcctagtg
      241 atataaccat tagtaataca aaagatatat ttcggttgca tattttacct atgtttggtg
      301 aatac
//
LOCUS       DAYYAG010000327          300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000327 DAYYAG010000000
VERSION     DAYYAG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.343"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           99 a           52 c           51 g           98 t
ORIGIN      
        1 atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat
       61 taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca atttttgaat
      121 aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt agcgacaaat
      181 acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc agcaaataat
      241 gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca
//
LOCUS       DAYYAG010000328          291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000328 DAYYAG010000000
VERSION     DAYYAG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.345"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACHARQ_003099"
     CDS             <1..>291
                     /locus_tag="ACHARQ_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8411423.1"
                     /translation="NGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSR
                     DALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGL"
BASE COUNT          100 a           39 c           58 g           94 t
ORIGIN      
        1 gtaatggaat aattccaagt gttagtggag atacgattcg gtctgaaaaa aattattcta
       61 tcattgtttt tgaaaagtta gcacaaacgt ctataacatt aggaatggat ataatagaag
      121 cgtatcaaag tagagatgct ttgatacaag aaaacgaatt agctgtatcg ttacctgagg
      181 tcttaaaagt gagggattct gggattgttt attatactaa ggaaatcgga aaaactaaga
      241 tagagcatct ttctccctta atttcttctg tcgttcaatt catcggctta a
//
LOCUS       DAYYAG010000329          275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000329 DAYYAG010000000
VERSION     DAYYAG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.348"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          114 a           30 c           49 g           82 t
ORIGIN      
        1 attcctttaa ggtgtcacta aaaattataa aaaatagtgt tgacgcactt ttgatgtcat
       61 gttataataa cgaagtcgtt tggatgagat agctaataaa gcttgatttt atcgaaaaaa
      121 acttcgaaaa agttattgac aaataacaat aaagatggta tgataatcaa gtcgctgcta
      181 atgagtggca aagacgttta gaaaaaataa acaaagaagt tgttgacatt ctcttctgaa
      241 tctgataaga ttaaagagtt gttaaaacaa aaaga
//
LOCUS       DAYYAG010000330          271 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000330 DAYYAG010000000
VERSION     DAYYAG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.349"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..>271
                     /locus_tag="ACHARQ_003100"
     CDS             8..>271
                     /locus_tag="ACHARQ_003100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8411424.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDV"
BASE COUNT          101 a           32 c           57 g           81 t
ORIGIN      
        1 aggagacatg gatgaaatga aactaattgg tatagattta gacggtactc ttttgaattc
       61 tgaccagagc attagtcaaa aaaatgcaag aacaatgaaa catttaccgg aagactgttt
      121 tccattcatt tgttctggaa gagaagtaga agacattaat aatattctca aaaaatctgg
      181 tttaaggata ccagcagtag ggttaaatgg ttctgttgga tttgatggag aaaaaaagat
      241 atttgagtct tccttcgata aaaatgatgt a
//
LOCUS       DAYYAG010000331          271 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000331 DAYYAG010000000
VERSION     DAYYAG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.350"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHARQ_003101"
     CDS             <1..156
                     /locus_tag="ACHARQ_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8411425.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT          106 a           22 c           56 g           87 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct t
//
LOCUS       DAYYAG010000332          266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000332 DAYYAG010000000
VERSION     DAYYAG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.denovo.352"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..>266)
                     /locus_tag="ACHARQ_003102"
     CDS             complement(36..>266)
                     /locus_tag="ACHARQ_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8411426.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaa
//
LOCUS       DAYYAG010000333        11363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.guided.088, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000333 DAYYAG010000000
VERSION     DAYYAG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.guided.088"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(371..607)
                     /locus_tag="ACHARQ_003103"
     CDS             complement(371..607)
                     /locus_tag="ACHARQ_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411427.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(790..1161)
                     /locus_tag="ACHARQ_003104"
     CDS             complement(790..1161)
                     /locus_tag="ACHARQ_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8411428.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1158..2486)
                     /locus_tag="ACHARQ_003105"
     CDS             complement(1158..2486)
                     /locus_tag="ACHARQ_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8411429.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACHARQ_003106"
     CDS             complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACHARQ_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8411430.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(3034..3404)
                     /locus_tag="ACHARQ_003107"
                     /pseudo
     CDS             complement(3034..3404)
                     /locus_tag="ACHARQ_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3549..3734
                     /locus_tag="ACHARQ_003108"
     CDS             3549..3734
                     /locus_tag="ACHARQ_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8411431.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3892..4059
                     /locus_tag="ACHARQ_003109"
     CDS             3892..4059
                     /locus_tag="ACHARQ_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411432.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4677..5336
                     /gene="vanR-B"
                     /locus_tag="ACHARQ_003110"
     CDS             4677..5336
                     /gene="vanR-B"
                     /locus_tag="ACHARQ_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8411433.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5339..6700
                     /gene="vanS-B"
                     /locus_tag="ACHARQ_003111"
     CDS             5339..6700
                     /gene="vanS-B"
                     /locus_tag="ACHARQ_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8411434.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            6855..7661
                     /locus_tag="ACHARQ_003112"
     CDS             6855..7661
                     /locus_tag="ACHARQ_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8411435.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            7679..8386
                     /locus_tag="ACHARQ_003113"
     CDS             7679..8386
                     /locus_tag="ACHARQ_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8411436.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            8383..9354
                     /locus_tag="ACHARQ_003114"
     CDS             8383..9354
                     /locus_tag="ACHARQ_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8411437.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            9347..10375
                     /gene="vanB"
                     /locus_tag="ACHARQ_003115"
     CDS             9347..10375
                     /gene="vanB"
                     /locus_tag="ACHARQ_003115"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8411438.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            10381..10989
                     /locus_tag="ACHARQ_003116"
     CDS             10381..10989
                     /locus_tag="ACHARQ_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8411439.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(11055..11228)
                     /locus_tag="ACHARQ_003117"
     CDS             complement(11055..11228)
                     /locus_tag="ACHARQ_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411440.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2973 a         2576 c         2913 g         2901 t
ORIGIN      
        1 ccctttccaa gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca
       61 cagacgaata acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc
      121 gtcttttctt tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga
      181 agtattcgct tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc
      241 cttaaatgcg taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg
      301 gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc
      361 ctcttgccgt ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg
      421 aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga
      481 tctccaccgt ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg
      541 cccacctctc accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc
      601 cgtccatcct gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg
      661 gaaccagaga aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg
      721 gcggggatag gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata
      781 taagggcttt tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc
      841 ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt
      901 cggcgtgaac gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat
      961 ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag
     1021 cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt
     1081 gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta
     1141 attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa
     1201 atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg
     1261 tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg
     1321 tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac
     1381 aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat
     1441 tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata
     1501 tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg
     1561 atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa
     1621 ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc
     1681 gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct
     1741 ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag
     1801 gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata
     1861 aactttcccg gcttgatctc atagccctgc gcctgcatga gccgcaggaa atcatcaaaa
     1921 tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc
     1981 cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc
     2041 acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc
     2101 tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga
     2161 ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc
     2221 acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg
     2281 gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc
     2341 agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc
     2401 ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg
     2461 ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt
     2521 cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc
     2581 cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat
     2641 agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca
     2701 ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc
     2761 gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac
     2821 aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca
     2881 ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac
     2941 tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc
     3001 agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt
     3061 cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc
     3121 ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat
     3181 ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac
     3241 cggcagactg ggaatggccc gtcgttttcg atgttggcaa gatagcgcca gtcaatattt
     3301 attttctctg ccattgcttt gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc
     3361 gcgccgaaag tttcaaagcc gggacaatct tcaatattcg ccatatcgca cctcccactt
     3421 acattgtata tttcatactc tgcccgtgga atgttggtat atgcaccttt cttaaagttt
     3481 atatttcaca ttttgtttct tttattcttc gagcaaaaga attataatgt accctgtgga
     3541 ggtgtactat ggactacatg acgttgaaag aggcaggcga gaaatggggt gtgcccccac
     3601 gatggataaa ctactattgt gctgcgggac gcatccccgg cgctgtaaag atggcgacga
     3661 tctggctcat tcccaaaact gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg
     3721 cggttactga atagtataat gaaaccggcg cattttggat atggttttga agagagagaa
     3781 agcatggtat tttgtcacgg aaaaactata agatgataga tggcaggctg ctccagaccg
     3841 ataaaaagta ttctcatctg aaacagaaag aaaagattta cacatggatg tatgaagaag
     3901 cgaaacttta tcatgacaga aatggaaaat gcccgaaaca aagacgagga agctgctgct
     3961 gtatcagccg tatatggtag aattgaaaag gccggcatct ggattcttta tggtgaagtc
     4021 tccagacatt ggataactgc tggtctaatg cgcctttgag tatggggtga ttgattttaa
     4081 taccggggag gtacggcttt ttgaggaaaa tattctggga tttacatttg gcgattatta
     4141 catttcctgt aactgcaagg acacggatga tgaagatatg aggaatatag acttgctgat
     4201 tttctactgt cttgaacggt attaggactg aatctgctgc gcggagtgcg gcaagagcga
     4261 ccggagggag tacacacagg ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc
     4321 aaaaaagagg ccgcgcattt tgctgaggct tggggtgtcc ccaacaagca aaacgagaac
     4381 gccgcaggcg ggcaagtggt ggatcagaat atccgtaaag gatattctga tccattgctt
     4441 gccagtatta tcgggatgtt ttgacaaccg ctgtaatcga aacatcccaa acattaagaa
     4501 ggtgttaatt attattatct tgatttaatt cgtttaaacc aataatatag atacatacac
     4561 catgaaaaga agcgagtatc ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg
     4621 aaactacagg gaaactacag actgttatgt tatcttctta aaatggaggg atttttatgt
     4681 cgatacgaat tctacttgtc gaggatgatg atcatatctg taatacagta agggcgtttc
     4741 tggctgaggc aggatatcag gtggatgcct gcacagatgg aaatgaggca tacaccaagt
     4801 tttacgaaaa cacttatcaa ctggttattc ttgatattat gctgcccggt atgaacgggc
     4861 atgaactttt gcgtgaattt cgtgcgaaaa atgatactcc cattctgatg atgacagccc
     4921 tgtcggatga cgaaaaccaa atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa
     4981 agccattcaa gatgcagatt ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg
     5041 cgctggcaaa ggaaatccgt gtcggcaggc tgacacttct gccggaggat ttcacggtac
     5101 tttgtgacgg tacagagctg cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg
     5161 tgcagaacaa aggcagaacc ttaacgcatg aaatcatttt gtcacgtata tggggatatg
     5221 actttgaggg tgatggcagc acagtccaca ctcatatcaa aaatttgcgg gccaagctgc
     5281 cggaaaatat catcaaaacc attcgcggtg taggataccg attggaggaa tcataataat
     5341 ggaaagaaaa gggattttca ttaaggtttt ttcctatacg atcattgtcc tgctactgct
     5401 tgtcggtgta acagcaacac tgtttgcaca gcaatttgtg tcttatttca gagtgatgga
     5461 attacagcaa acagtaaaat cctatcagcc attggtggaa ctgattcaga atagcgatag
     5521 gcttgatatt caagaggtgg cagggctgtt tcactacaat aaccaatcct ttgagtttta
     5581 tattgaagat aaagagggaa gcgtactcta tgccacacca aatgccaata catcaaatag
     5641 ttttagaccc aactttcttt atgtggtaca tagagatgat aatatttcga ttgttgctca
     5701 aagcaaggca ggtgtgggat tgctttatca agggctgaca attcggggaa ttgttatgat
     5761 tgcgataatg gttgtattca gccttttatg cgcgtatatc tttgcgcggc aaatgacaac
     5821 gccgatcaaa gccttagcgg acagtgcgaa taaaatggca aacctgaaag atgtaccgcc
     5881 gccgctggag cgaaaggatg agcttggcgc actggctcat gatatgcatt ccatgtatgt
     5941 caggctgaaa gaaaccatcg caaggctgga ggatgaaatc gcaagggaac atgagttgga
     6001 ggaaacacag cgatatttct ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc
     6061 tacaagcgtt ctgttggagg gaatgcttga aaatatcggt gactacaaag atcattctaa
     6121 gtatctgcgc gaatgcatca aaatgatgga taggcagggc aaaatcattt ccgaaatact
     6181 ggagcttgtc agcctgaatg atgggagaat cgtacccata gctgaaccgt tggacatagg
     6241 gcgcacggtt gccgagttgc tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg
     6301 gttcgtcaca gatattccag ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa
     6361 ggcactatcc aatgtcatat tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg
     6421 gatatggagt gagcctggtg ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca
     6481 cattgatgat actgcgcttc caaggctgtt caccccattc tatcgcattg atcaggcgcg
     6541 aagcagaaaa agtgggcgaa gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt
     6601 acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct cggatggcgt
     6661 tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa cttaaatgat
     6721 ttccctgccg gtcttttttt gctgctacag gaaaactaca gactggctac agggaaagta
     6781 cagatgcgct tgccataata acaatcgtac cagccacaaa tcgtagtttt attgcaaagg
     6841 aggcattcga tcaaatggaa aaaagcaact atcattccaa tgcggatcat cacaaacggc
     6901 atatgaaaca atccgtggaa aagcgggctt ttctatgtgc gttcattatt tcgttcacag
     6961 tctgcacgct gtttttgggg tggagactgg cttccgtatt ggaggcaaca cagataccgc
     7021 ccatccctgc aactcataca ggcagcagca ctgacgtagt ggagaatttg gaggaaaacg
     7081 ctcttgccac cgccaaagaa cagggagatg aacaggaatg gagcctgatt ttagtgaaca
     7141 ggcagaaccc catccccgca cagtacgatg tggagcttga gcaactatca aatggtgagc
     7201 ggatagatat tcggatttct ccctatcttc aagatttgtt tgatgccgca agaactgatg
     7261 gagtttaccc gattgtcgca tccggatacc gaacaacaga aaaacagcaa gaaattatgg
     7321 atgaaaaaat tgccgaatat aaggcgaaag gctacacctc tgcacaggct aaagcggaag
     7381 cagaaacttg ggtggccgtg ccgggaacga gcgagcatca gcttggtctt gctgtggata
     7441 tcaatgcgga cggaattcat tcaacaggca acgaggttta tagatggctg gatgaaaaca
     7501 gctatcgttt tggttttatt cgtcgctacc caccagacaa gacagagata accggtgtga
     7561 gcaacgagcc gtggcattac cgatatgttg gcatcgaagc tgccacagag atgtacaacc
     7621 aaggggtttg ccttgaggaa tatttaaaac cagaaaaatg agaaaaggat gttatgttat
     7681 ggacagaaaa agattgacac agcgtttccc gttcctgctt ccaatgagac gggcgcagag
     7741 aaaaatgtgc ttttatgcgg gaatgagatt tgacggctgt cgctatgcgc agacgatagg
     7801 ggaaaaatcg ctttcccatt tgctctttga aacggattgc gcattataca accacaatac
     7861 cggatttgac atgatatacc aggaaaacaa ggtgttcaac ttaaagctgg cagcaaagac
     7921 cttaaacggc ttattgataa gaccggggga aaccttttct ttctggttgt tcctgcatac
     7981 gccattgaca attatccagc gcagaggtca cgaagtaaag gagtttccag agccaaacag
     8041 tgacgagatt aaaggggtgg atgcaaccat ttcagagggc tggattgatt taaaagtgcg
     8101 aaacgatacc gactgcacct accaaatatg ggtgacctta gatgatgaga aaatcatcgg
     8161 tcaggtgttc gccgacaaag agccccaagc attatacaaa attacaaatg gcagtatcca
     8221 gtatgtccgt gaaagtggcg ggatttatga atatgcccag gttaaacgga tgcaagttgc
     8281 cttaggtacc ggggaaataa tagattgcaa gctgctttat acaaacaaat gcaaaatctg
     8341 ttatcccctc ccggaaagtg tggatattca ggaggagaac caatgagaaa aagtgtgggc
     8401 attactgttt ttggatgcga acaggatgag gcaaatgctt tccgcgcttt atcgccggat
     8461 tttcatatta tccctacgct gattagcgat gcgatatcgg cagacaacgc aaaattggcc
     8521 gctggcaatc aatgcgttag cgtaagccat aagtccgagg tttccgaggc gacaattctt
     8581 gcgctgagaa aggtcggggt aaaatacatt tctacccgca gcatcggctg cgatcacatt
     8641 gatacgactg ccgccgagag aatgggaatc tcggttggca cggttgcgta ttcgccggac
     8701 agcgttgcgg attatgcttt gatgctgatg ctgatggcca tacggggtgc aaaacccacc
     8761 atgcacgccg tggcgcaaca agatttcaga ttggatcgta tccgggggaa agaactgggg
     8821 gatatgactg tgggagttat tggaaccggc catatcgggc aagcggtcgt caaaaggctg
     8881 cggggatttg gatgccatgt gctggcctat gataacagcc gaaaaatgga tgcagattat
     8941 gtccagcttg atgagcttct aaaaaacagc gatattgtta cgctccatgt gccgctttgt
     9001 gcggataccc gccatctgat cggtcagaag caaattggag agatgaagca aggcgcattt
     9061 ttaatcaaca ccgggcgcgg agcgcttgtc gataccgggg cgctggtgga ggcgttggaa
     9121 agcggaaagc tgggtggtgc ggcactggat gtgttggagg gcgaggatca gtttgtttat
     9181 accgattgct cgcagaaagt gcttgaccat ccctttttgt cgcagctttt aaggatgccg
     9241 aatgtgatca tcacacccca tacggcgtac tacactgagc gtgtgctgca ggatactaca
     9301 gaaaaaacaa tcaggaattg tctcaatttt gaaaggagtt tacagcatga atagaataaa
     9361 agtcgcaatc atcttcggcg gttgctcgga ggaacatgat gtgtcggtaa aagccgcaat
     9421 agaaattgct gcgaacattg atacggaaaa attcgatccg cactacatcg gaattacaaa
     9481 aaacggtgta tggaagctat gcaagaagcc atgtacggaa tgggaagccg acagtctccc
     9541 cgccatactc tccccggata ggaaaacgca tgggctgctt gtcatgaaag aaagcgaata
     9601 cgaaacacgg cgtattgatg tggctttccc ggttttgcat ggcaaatgcg gggaggatgg
     9661 tgcgatacag gggctgtttg tattgtctgg tatcccctat gtgggctgtg atattcaaag
     9721 ctccgcagct tgcatggaca aatcactggc ctacattctt acaaaaaatg cgggcatcgc
     9781 cgttcccgaa tttcaaatga ttgataaagg tgacaagccg gaggcgggtg cgcttaccta
     9841 ccctgtcttt gtgaagccgg cacggtcagg ttcgtccttt ggcgtaacca aagtaaacgg
     9901 tacggaagaa cttaacgctg cgatagaagc ggcaggacaa tatgatggaa aaatcttaat
     9961 tgagcaagcg atttcgggct gtgaggtcgg gtgtgcggtc atggggaacg aggatgattt
    10021 gattgtcggc gaagtggatc aaatccggct gagccacggt atcttccgca tccatcagga
    10081 aaacgagccg gaaaaaggct cagaaaatgc gatgattaca gttcccgcag acattccggt
    10141 cgaggaacga aatcgggtgc aggaaacggc aaagaaagta tatcgggtgc ttggatgcag
    10201 agggcttgcc cgtgttgatc tttttttgca ggaggatggc ggcatcgttc taaatgaggt
    10261 caataccctg cccggtttta catcgtacag ccgctaccca cgtatgatgg ccgccgcagg
    10321 aatcacgctt cctgcactga ttgacagcct gattacattg gcgttaaaga ggtgataagt
    10381 atggaaaatg gttttttgtt tttagatgaa atgttgcatg gcgtccgttg ggatgccaag
    10441 tacgccacat gggataactt cacaggaaaa ccggtggatg ggtatgaggt gaatcgcatc
    10501 atcggcacaa aggccgtggc gtttgctctg cgcgaggcac aaatccatgc ggctgcgctt
    10561 ggctatggct tgcttttatg ggatggatat cggccaagaa ctgcggtgga ctgcttcctg
    10621 cgttgggcag cgcaaccgga ggacaatctc acaaaagaaa aattttaccc caatatagag
    10681 cgagccgagt tgattacaaa gggttatgtg gcctcacaat ccagccatag ccgtggaagc
    10741 gcaattgatc ttacgctcta ccacctggat acaggggaac ttgtttcaat gggaagtaac
    10801 ttcgatttta tggacgaacg gtcgcatcat acagcaaaag ggatagggga tgcagaggca
    10861 caaaatcgaa gatgcttgcg taaaatcatg gaaagcagcg gatttcagtc ttatcgcttt
    10921 gaatggtggc actataagtt gattgatgag ccataccccg atacctattt taattttgcc
    10981 gtttcataat gaaagtattg gttttgtatc agattttgga tttgataaaa tctatgctgg
    11041 tcatggacaa tcttttactg gaaagtggtt aaagcatctt ttgtaataca ttgcaaatta
    11101 tagtattcaa tctgcccagc ggcagaaaag aaaaaaaccg ctgttgggca gacctttccc
    11161 acgcccattt tgtattttgc tggcggcggt tttgagaaat caaggccgcc gttcttttct
    11221 ggcagcaaaa ggtgtggcgg cccataggag gatgccgcta tgctgttttg ctattgccat
    11281 gctctaatcc acttccacca ctcaaaaaaa gccttgcccc tccatgaact gaaccccaaa
    11341 agttgaacta tttaatggac tgt
//
LOCUS       DAYYAG010000334        10613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.guided.094, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000334 DAYYAG010000000
VERSION     DAYYAG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.guided.094"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..1072
                     /locus_tag="ACHARQ_003118"
     CDS             176..1072
                     /locus_tag="ACHARQ_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8411441.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1222..2163)
                     /locus_tag="ACHARQ_003119"
     CDS             complement(1222..2163)
                     /locus_tag="ACHARQ_003119"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8411442.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2289..3410
                     /locus_tag="ACHARQ_003120"
     CDS             2289..3410
                     /locus_tag="ACHARQ_003120"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8411443.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3668..5170
                     /gene="eat(A)"
                     /locus_tag="ACHARQ_003121"
     CDS             3668..5170
                     /gene="eat(A)"
                     /locus_tag="ACHARQ_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8411444.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5237..5764
                     /locus_tag="ACHARQ_003122"
     CDS             5237..5764
                     /locus_tag="ACHARQ_003122"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8411445.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5975..7036
                     /locus_tag="ACHARQ_003123"
     CDS             5975..7036
                     /locus_tag="ACHARQ_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8411446.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7039..7935
                     /gene="murQ"
                     /locus_tag="ACHARQ_003124"
     CDS             7039..7935
                     /gene="murQ"
                     /locus_tag="ACHARQ_003124"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8411447.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7949..9931
                     /locus_tag="ACHARQ_003125"
     CDS             7949..9931
                     /locus_tag="ACHARQ_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8411448.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9996..10442)
                     /gene="nrdI"
                     /locus_tag="ACHARQ_003126"
     CDS             complement(9996..10442)
                     /gene="nrdI"
                     /locus_tag="ACHARQ_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8411449.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3474 a         1861 c         2287 g         2991 t
ORIGIN      
        1 aattacaact attcagaaaa cttttaggcg aattttaata gaagaatgat ttatttcatc
       61 atattttcat acaaatgtta aattttcata caaatatagg aaacgtttta ctggcttttt
      121 actatttaaa caggctatca taggaaaaag tgacagagaa agtgaggcgg aaaagatgaa
      181 caaaaatgac gtactgctag ttgcaaaaaa taaatcactt tattttttgc cttccatccg
      241 ctctttagat gtttggtccg gaaaagattt actacagtcg gaattcatta aatcagctag
      301 acaacagttg gcaaattatc agctgatcgt ttttttagat tatggatttg atatggagat
      361 ggctcgacgc gtgcgcccat ttaccaatgc aaaaattgtg ttattttttt ggaatcactt
      421 caaagcagaa cacgaggcga tgctgaagca ggcacaagat gaaccggcaa tcgatgagat
      481 ttatcatttt gattttttgg aagcaaaaga acttgggctg aaacataata gttcatttta
      541 ttcgaaacat atgaattttt cttctagcga accaaagacg gatctatttt ttggtgcaac
      601 gaataatggg agaaaagaac gagcagaaag ctataagcag gaatttataa aaagagatgt
      661 tacgaccaac tatttcattc ttccacatcg agggaatgaa caacctggat atcttactta
      721 tgaagagtac ctgaaaaaaa caggggaatc gcgcgggatt ctggaactgt tgcgggaggg
      781 tcagcaagga gtgactttgc ggacatttga atccatgtat tttcaaaaga aactagtgac
      841 ggataatcaa gcgattgctc attatcattt ttatcgtcct gaaaatattt ttttgcttcg
      901 agaacgtgat ttagatgaat taccggaatt tctgcataca ccatttaagc caatcctatc
      961 agagctattg gaattttatg atgcagaaaa ttgggcaaaa cgatttatca ctaacgattc
     1021 cactatttat gagcaatacg aatatgacct ctcctctgtt gaaaacaaat aaaaaaccta
     1081 cattttgaag agattatgta ctgaaacaaa caagcacaat tcttatggca tcatgagagt
     1141 tgtgttttct ttttgatctt ttattgggct taaaaaaaga gaaccagctg ctgattcagc
     1201 tgattctcat cagagagcct tttattctgc gccaaggatt ccttcttttg catggactaa
     1261 ttgggtcaaa aaggcacaat atggtgtagc aatatcatat ttttgtcctt ttcgccaaat
     1321 ggcaccattg atgtagtcga tttcagtcaa acgatggttt ttgatcaggt cttggtacat
     1381 agatgggtaa tgcaacccga tgttgtcagg atcataagta gaagcaatat gttggtacac
     1441 ttcttcttga tctagtatga ttccttcttt tgctgcgata gaagcaaatt cagatacgat
     1501 tgttcgaacc atgctctctg ccgctttttg cgcgcctagt tcagcaatat tacaatccaa
     1561 aatcgtgcat aaaccattca atgttccatt tacacaagct tttcgccaaa tggaataacg
     1621 aacgttatgg ctatagatcg gatttaaatt tgcttcttga aagacttcta caactttttt
     1681 tgtaaatgct tcgccttctg gttccaagtt ctctagttcg atttctccat cacctaacaa
     1741 tgtgactttt ccgggaccag ctagaccagc tgtccacatc gtgatcccaa ataagatatt
     1801 tttctttggc acatattttt ccagtacatc ttcatgacct aatccattta gtaaacacaa
     1861 aacataagta ttgtcagtaa taatggattg gatcgaacaa aacatatcat ctaattgatt
     1921 ggcttttgtt aatgctacga tcaagtccac gtgttcgttg ctttcgatga tttcttctgg
     1981 tgaatagatt ggcagttttg cgaccacttc tttaccgtta aagtcagcaa tcaacccatt
     2041 tgtacgaatg gcttcaatgt gagcaggcca acgatcgatc aagaggacct catttccgct
     2101 ttgatgtagc atcaatccga ttcggctgcc cattgctccg gccccagcta ttgcgatttt
     2161 catgtttttt ctctccttta ttcaatgagt tcccttttag tatatagaaa aatggaagaa
     2221 aatagcattg ttttgtttga aaaaaatgaa aaaaagtgtc ttacttaaga agaaagtgag
     2281 ggatacagat ggatattttg gcggcaattg attcatttaa aggaagtgct tcttcatttg
     2341 aactaaatca ggcagttcta aatggctggg aagatcgtaa aggaagaaaa atcaatgtgc
     2401 cgattgcaga tggcggagaa aatactgcag aagcaatcta ttacgcgctc aggggaacat
     2461 ggcgtaccat tgatgggatg gatctcttat tccgtcagcg aaaaatcagc tatttattga
     2521 caacttggca aggaaaaaac accgcagtca tcgaatctgc acaagcattg gggttagatc
     2581 tacttgataa accaacggat caaactatcc gacttgcttc cagttatgcc ttaggagaac
     2641 ttattaaaga tgcactgctt caagaagtag aacaaattat tgtcacttta ggaggaagcg
     2701 ggtgtagcga tggtggtcta ggattactcc aatccttggg tgcttcttta gaagggtaca
     2761 cagaaggaaa tcctttatta tctactaaaa aacttcattt tgatcgaaga aacgttcttg
     2821 tttctaaagt aaaattgatt atcgctgcag atgttaccaa tccatataca ggtcaacaag
     2881 gagctgcttg gcaatttgga agacaaaaag gaggaataga tacgacgcta gctttcttag
     2941 ataaacaagc aggaaagatt gctcagcaaa taaaagattt ctgggaggtg gatttagatg
     3001 atattccagg aagtggagca gcaggcggta ttggtggagc cctttttttg ttgggagcga
     3061 agatggtttc tggtttcgat ttagtcagcc aaatcactgg attggaaaaa aagatcgaac
     3121 aagcagatgt catcattaca ggagaagggc ggatcgatca gcaaacgatc cacggaaaag
     3181 ttccatacgg tgtagcagtg cttgcaaaaa aatatcgaaa accaataatc gctatttgcg
     3241 gaagtagaga ccaagagcta gaaggacttt ctgattttct cccaattgtc ttaagtattc
     3301 atttgggtcc aataggttta gaagaagcaa tggaacacag gcaaacatta gaaaaaacaa
     3361 aaatcttagg acagacactt agcagatttt tcaccatcct cccgaagtag ttgacaaaat
     3421 cattttcttt atgctatcgt aatttcaaat ataggacaaa gtgagggatc cgtgcattga
     3481 aaaattaagt cgattgatga aagctgcatt cagactgtac cttgtggaga caaggaagca
     3541 gtgtgcagat tttcgggatt ggcttggttt tttcgatgta tgggtctttt tgtgtgtatc
     3601 caacccttgc atcctcttta gccagttctg aaaataggac tggtcttttt ttacgaggtg
     3661 atggaaaatg tctaaaatcg aaataaaaaa tctgacattc ggctacgaca gccaaggcac
     3721 attattattt gaacaagcaa atctaaattt tgacacacaa tggaaactag gacttatcgg
     3781 acgaaacggt cgaggaaaga caactttact gaatattcta caaaacaaac taccttatca
     3841 agggcaagta atccatcagc aagaatttgc ctatttcccg caacagacaa aagataaaga
     3901 acgtttaacc tattacgtgt taaatgatat tacggatttt gagatatggg aaatcgaaag
     3961 agagctccaa ttgatgcaaa cagatcctga aatcttatgg agagaattca gcacactatc
     4021 ggggggagag aagacaaaag tcctactggc acttttattt gtggatgaca ctcatttccc
     4081 gttaatcgat gaaccaacga atcatttgga tatctctggt agaaaacaag tagcggctta
     4141 tttgaaaaag aaaaaacaag gcttcatcgt ggtcagccat gaccggggat ttatcgatga
     4201 agtagtggac catgttttag caatcgaaaa aagtcaactg gaactttatc aagggaattt
     4261 ctctatctat gaagaacaga aaaaacttcg tgatgaattt gaaatggctc aaaatgaaaa
     4321 attgaaaaaa gaagtcagta ggctaaagaa aacagcagct gaaaaagccg aatggtctcg
     4381 ttcccgagaa ggagataaaa caaagaaaca agtcggattc atcgatactg aatctagacg
     4441 agtgaataaa ggagcagtgg gtgctgatgc tgcacggacg atgaaacgat ccaaagcaat
     4501 cgtgaatcgg atggagaccc agatcagcga gaaagaaaaa ctattaaaag atatcgaata
     4561 tatcgattct ttgacgatga atagccaagc gtctcaccat aagcgacttt taagcgtaga
     4621 agatcttcaa ttagggtatg aaaatctgtt attcgagcca attcatttta caatcgagcc
     4681 tcatcagcgg gtggcgattt caggtcctaa cggtgcagga aagtcatcca ttatccatta
     4741 tcttctgggg gcattcaacg gcaaggttat aggagaaaaa taccagccaa aacatctgag
     4801 cattagttat gcaagccaaa attatgaaga caatcgagga acgttggcgg aatttgcaga
     4861 gaaaaaccaa gtagactacc aagcattttt gaacaacctc cgaaagcttg ggatggaaag
     4921 agatgttttt cataacaaga tcgagcagat gagtatgggc caacggaaaa aagtggaatt
     4981 ggctaaatct ttatcacagc cagctgaact atatacatgg gatgaaccat tgaattattt
     5041 ggatgtcttc aatcaagaac aattagaaca actgatcttg aacgtgaaac ctgccatgtt
     5101 actagtggaa catgatcaaa ccttcctgga taaagtatct actgagatta tttctcttga
     5161 gagaatctaa cccttgtaat ctcaagaaca acaaggtatg gttaagggga actttgccaa
     5221 cgaaggagag aatcacatgc tttatgtaac gagacacgga gaaacaactt ggaacgcaca
     5281 aggattagtt tgcggacggg cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca
     5341 gaaactagca gaaaaggtag tagatttgcc tgttccaatc acgaaaatca tccattcccc
     5401 tcttcaacga gcaagagata cagcacaagc agttgctgat cgtctcagct tgccgctgac
     5461 cgttgatgaa cgattggtag aaatggattt tggggattat gatggactgc caagcaaaga
     5521 tgagaacttt caaaaagcac gtttagcgtt cgctgtacga ttcccaaatg gggagtcagt
     5581 cttagatgtg tatgcacgga tcgttccttt attgaaagaa tgcatcgaag acgaagaaaa
     5641 tgtctatctg ttagtttgcc ataatgcctt gataagagtg atcaatgctt attttcatcc
     5701 gatgcctaat gaggggttct ttacattcat ggtagataat acagaattgg tttcttatga
     5761 gtaattatgt ttttgtgtta tttaaataaa atatttcaag gcactgcttt tgcttagcag
     5821 tgcctttttt aggttatgac aattcttttg ataatataaa ctcaaccttt agaaatgaaa
     5881 tttaattatt ttaataaaag tattgaaatt ttgtttcttt agatatatac tctagtcata
     5941 gaaatgaaga cggatacaga aaaagaggga taatatgttt ggcttttcaa tatttatgaa
     6001 cgattctttg tcagaagaga agaaacagta tatccgccaa atggcatcca atggctttgt
     6061 cggtattttc acttccatgc atataccaga agatgatgtt tcggcatatc aaaagcgatt
     6121 gatcgaacta ggaagcgaag cgaaaaagaa taaactagaa ttgatggtcg atatttcagg
     6181 agaggcattg gaacgagcgg gtttttcatt agaagatcta acgcctttga aagcaattgg
     6241 tgtaacaggt ttaagaatgg attatcatat ttccaatcag cagattgccg actggtcaca
     6301 tcaattgaaa atcagtttga atgctagcac gatcacgcct aaagatatcg atgaattgaa
     6361 agaagcagaa gctgatttct cacagattga agcttggcat aattattatc cacgtcccga
     6421 aacagggctg gacaaagaat ggtatcagaa aaaaaatcaa tggctcaaaa ttcaaggtct
     6481 ccttgtccaa ggttttgtac caggagatac agagttgaga ggcccactat atcaaggatt
     6541 gccaactttg gaagaacatc gagggatcca tcctcttgca ggcgctctag atctattagc
     6601 aagtaacacg gatattgttt acatcggaga tgcaggtctg tcagaaaacg ttcaggagca
     6661 atttgcttct ttccaaaaag aacagacagt cttgctccat acggaacctg tagatgaaga
     6721 attttacgaa tatatattag ggaagcatac gaatcggcaa gacgatgcaa gagatgtcat
     6781 acgaagcgta gatgcacgtt ttcgagagat tccgcctatc ccagcaagaa atacagctac
     6841 gcgtatgaaa gggtctatta ctttagataa cgaaaagtat ttgcgctata tgggtgagat
     6901 ccaattaacc aaatatgatt tgccagcgga tgaaaaagtg aatgtagtag ctaaagttat
     6961 caaagaagag ctaccattga tcaaccaaat caaagctggt atgaactatc aatttataag
     7021 aaaagaagga agataaaaat gagcaaaatc aatttagaga acctaacaac ggaacgcaga
     7081 aaccaagaaa cttttggatt agatgagatg agtatttctc aagccgttca actaatgaac
     7141 aaagaagatc agaaagtggc cgctgcggtt gaaaaagaac tgacaagtat cgaaccagtg
     7201 attgctaaca cgattgagtc ctttaaaaaa ggtggacgtc tgatttatat gggagccgga
     7261 acaagtggaa gactaggtgt gttagatgct gctgaatgcg taccgacatt cggtgtagaa
     7321 ccggaaatgg tcataggtct aattgctggt ggagaagaag caatgactgt agctgtcgaa
     7381 ggggccgaag attcttatga gctagggcaa gaagatctaa tcaagctgaa tttgacaaaa
     7441 aatgatatag ttattggtat cgcagctagc ggtcgtacac catatgtgat cggtggatta
     7501 gattacgcaa aatcgatcgg tgctgtcaca ggaacgattt cttgcaataa acaagccgaa
     7561 atcagtaaac acgcggattt cccaatcgaa gtcgatgcag gtccagaatt tttgacaggc
     7621 tctactcgat tgaagtcagg aacagcacaa aaacttatct tgaatatgat ttctactatc
     7681 agtatgattg gtataggtaa agtattcaat aacttgatgg tcgatgtcaa accaacaaat
     7741 gaaaaactaa tcgaacgctc aaaacgaatc atcatgcaag cgacagaagc tgattacgaa
     7801 acagctgaaa agtactttga agaagcagat caaaatgtta agctggctat cgtcatgatt
     7861 ttgacaaata gtgataaaga ggaagctcaa gcaaaattag ctgaagcaaa cggatttgta
     7921 aaagggacct tatgaggggg aaaagaccat ggcaaaaaac gaactgactt tggaagaact
     7981 ggcaaaacaa atttataaag gggcaggcgg catgggcaat gtagaaagca ttgtccactg
     8041 catgactcgt gtgcgtatga agatcatcaa tgatgatctt gtaaatgtct ctgaattaaa
     8101 agcgattcca ggagtgcttg gtgtagtaga agatgaacag cttcaagtca tcatcggacc
     8161 tgggaaagtg aacaaagtag cacagcaaat ggttgatttg gcaggcgtga aattaggaga
     8221 aaaactttct tctgtaagta cagctacagc aaattcatca gcatctggaa aagaccgtgt
     8281 aaacgaacgg gcacaagaaa tgaaagcggc acaaaaatcg aaacaaaaac catctaaaat
     8341 caaatcggta ttaaaagaca tttctaatat ctttgtgcca atgattcctg catttgtcgg
     8401 tactgggatc gtggcaggga ttgccgcagt tttagcaaac ttagtcacag ccggaacact
     8461 tgatgcagca acatggcaac aatatatcga tgtgatgaac atcttgaaaa acgggatgtt
     8521 tagctaccta gttatctata caggtatcaa tgctgcacaa gtttttggtg caacaccaac
     8581 tcttggtgga gttatcgggg ccgttgtgat gctgacaggg atgaatccgg aagcaccact
     8641 gaaaaactta tttactggtg aagctcttgc agctggacaa ggtgggatcc taggtgtcat
     8701 cttcgctgtt tggctattgt caatcgttga gaaaaaactg cataaagtag tacctgatgc
     8761 ggtagatatc atcgttacac caactctttc attgattgcc atcggtctga ttgaaatttt
     8821 cttcatcatg cctttagcag gtttcatttc tgatggcatg gtcggtggga tcaactgggt
     8881 acttggcgtt ggtggagcat ttgccggctt cgttctaggc acattattct tgccgatggt
     8941 tatgttcgga ctgcatcaaa ttttgactcc aatccatgtg caaatgattg atgaaactgg
     9001 tcgtacgcta ttgcttccaa ttcttgcgat ggctggtgca ggacaagtgg gtgcagcatt
     9061 ggcattatgg attcgctgca aaaaagataa agaattaaca gaaatgatca aaggagcttt
     9121 accagtcggg atcctaggaa tcggcgaacc attgatttac ggagtaacat tacctctagg
     9181 acgcccattt attactgctt gtatcggtgg aggtatcggc ggggcagtga tcggaatgat
     9241 cggaaatgtc ggggctatcg ctattggacc ttcaggagcc gctttgatcc cattgatctc
     9301 tgacggtaaa tggttaggct acatccttgg attattggca gcttatgctg gtggcttcgt
     9361 tgctacattc ttcttcggta ttcctaaaga ccgtttagaa aaagaagaat tagcgactga
     9421 aacagccaca gtaaatgaag tgcctgaaaa agtagtacct caaacttcag ctgaaagtgt
     9481 gactatcctc tcatcagttg ctgacggaac agttgtttct ttagataaag caagtgatcc
     9541 agtttttgca caaaagatga tgggtgaagg ctactttgta gaaccagaaa acggacatat
     9601 ctattctcca gttactgggg tagtcagctc tgtcttccca acgaaacacg ctattgggat
     9661 cacaacgcca aatggtttag aaatcttgct tcatatggga atcaacacag ttgacttagg
     9721 cggtacgcca tttgatgtga aagtaacaga agggcagcaa gtgacacctg atacgcttgt
     9781 agcagaagct gacttagaag cgatcaaagc agcaggcaaa gaaacatcca tgatcgtctt
     9841 ggtaaccaat atggatcgtg taaaaaactt tgtcttggaa aaaacaggta aagcaaaagc
     9901 aaaagcacct gtgatggatg tcaaagctta atatgataga aacaatagaa acgaaagaag
     9961 aaactgaaat ctatttttca gcttcttctt ttttattatt tattgttttc tgctaaaatc
    10021 tgataaattc gtgtcacatc agatggtgtt ccccgtaatt cataatctgc caagaaagga
    10081 aagccaaatg tttgtgcata ttgtttggct gttagacaat actgattatt gaagttttta
    10141 tttccgctac cgactacacc aagacaaagt ttatgattgt cttcatgttc cagatattca
    10201 cgcatcgttt cggtcaagat ttctgtatca ccgttatcta aaccatttcc gccgtctaaa
    10261 taagtaggaa cgaaagtaaa aaatggttct gtttctttcg taaaatcact attttcatgg
    10321 atttctttta aagtaacttc aggattgaca ggatcttctg catgcatttt ttcagcatat
    10381 tctgctagat gtttggcaaa ggcacgcgta tttcctgaaa tcgatatata taaaatgttc
    10441 atggatcatc ctccaattct ttccttttag tatagcatct tttttaagtt ttttggtggt
    10501 gtaatttgga aagggactag gcgctttgct tgtataatga gaaaaaagcc ggtagaatta
    10561 tatttctatt aaagttttta ttaattaagt aaaaataaaa aaggtttgtt ata
//
LOCUS       DAYYAG010000335         8928 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.guided.117, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000335 DAYYAG010000000
VERSION     DAYYAG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.guided.117"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..657
                     /locus_tag="ACHARQ_003127"
     CDS             88..657
                     /locus_tag="ACHARQ_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8411450.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            724..1461
                     /locus_tag="ACHARQ_003128"
     CDS             724..1461
                     /locus_tag="ACHARQ_003128"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8411451.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1692..2960
                     /locus_tag="ACHARQ_003129"
     CDS             1692..2960
                     /locus_tag="ACHARQ_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8411452.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3447..3911
                     /locus_tag="ACHARQ_003130"
     CDS             3447..3911
                     /locus_tag="ACHARQ_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8411453.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3895..4524
                     /locus_tag="ACHARQ_003131"
     CDS             3895..4524
                     /locus_tag="ACHARQ_003131"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8411454.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            4878..6356
                     /gene="msr(C)"
                     /locus_tag="ACHARQ_003132"
     CDS             4878..6356
                     /gene="msr(C)"
                     /locus_tag="ACHARQ_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8411455.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6403..6849
                     /locus_tag="ACHARQ_003133"
     CDS             6403..6849
                     /locus_tag="ACHARQ_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8411456.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7048..7563)
                     /locus_tag="ACHARQ_003134"
     CDS             complement(7048..7563)
                     /locus_tag="ACHARQ_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8411457.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            7680..8882
                     /locus_tag="ACHARQ_003135"
     CDS             7680..8882
                     /locus_tag="ACHARQ_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8411458.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2876 a         1553 c         1867 g         2632 t
ORIGIN      
        1 agttaaaagg aatagttaag tatgttttat aagtattaga catcataagc cgtccgttat
       61 aaagtaagga taaaaggagg gagcaaaatg acaagcctca tcgaaaaaca aatcgtcaat
      121 aaagagattc gagtaacgga tccgctcttt gaagtgatcc atcagatcca agcggaaaac
      181 gaagagcctt tagcagcgct gaatactggg taccatgaac cagcagatat ccgcaaacgg
      241 ctagaaaata tcattagtga caaagtagat gatacggtga ctgttctgct gcctttttat
      301 acagatttcg gcaaacatat tagtataggg aaaaatgtct ttatcaatcg tcaagtgatg
      361 ttcgttgact taggtggcat ctgtttagaa gattcagtat tgattggtcc tcgtgtcaac
      421 ctcatcacgg tgaaccatct aaccgatcca aaagaacgaa gaggcttgtc agtaaagcca
      481 atccacatca agaaaaatgc ttggatcgga gcaggagcaa ctattctgcc aggcgtaacg
      541 attggcgaga atgccattgt tgcagcgaat gcaactgtta cgaaagatgt cccagacaat
      601 accatcgttg caggaatccc tgccaaaatc gtcaaacctg tcgaacggat tatttgaaca
      661 gtaagatgaa ataagagata gtgagtataa gcaagagatt tttcaaaaca ggaggagaat
      721 agaatgaatg atttatcaga aaaagtgatt gtcatcatgg gcgcttcaag cggaatcggt
      781 gaagcaacaa cgaagctatt agcagaaaaa ggggctaaac tagttatagc tgcacgccga
      841 gaagatcgcc taaaagcaat aaaagaatct ttaccagaag ctgagctata tattcaaaca
      901 gcagatgtaa gagattttgc acaagtccaa gcagttatcg atttagcgat ggaaaagttc
      961 ggtcgaatcg atgtattgta caataatgca ggaatcatgc caaccgctcc attagttgaa
     1021 ggtcaccgtg atgaatggca aaatatgctt gatatcaaca tcatgggtgt gttaaatgga
     1081 atttccgccg tacttccaat catggaaaaa caaaaatctg ggcatatcat ttcaactgat
     1141 tctgtggcgg gacatgttgt ttatcctgat tccgccgttt attgcggaac aaaatttgct
     1201 gtccgtgcaa tcatggaagg attacgtcaa gagcaacggc aaaataacat caaatctacg
     1261 atcatctctc caggagctgt tcaaactgaa ttatatcaaa ccatcagcga caaaaaggct
     1321 gctcttgaac ttcatgaagc gcaaaaagaa tggggattaa catctgaaga tatagcttct
     1381 gctgtagcat ttgcgataga aacgccagac cggatgtctg tcagcaacat gattatccgt
     1441 ccgacgacac aggaagtata atccataacg gataatatcc agagagaatt acttgttaaa
     1501 tagttattca aacgaccatc aatttaaggc gtgaatggag aagaatattc ttcatttacg
     1561 tctttttatg cagaaaaagc aaatttatta aaaagaaaca aatgttattt aaatatggac
     1621 attgacttgc ttttactttt ttagttacac tgttatttag gaaaagtttc ataattcaaa
     1681 ggagaaaaaa gatgaaagca aagaatttat tatttagcgt tattctttta ggaaatctag
     1741 cagttgccaa cacaacagtg ctagctgaag aatcgacaga aactgtcgca actcagacat
     1801 cgagttcttc agaaacagga attgaaaacg atacagaaag tactttgcag tcagctactg
     1861 tctcagaaaa tacaatttca accgaagaac caacaactga aacaacagaa caaacacaaa
     1921 ccgaagacag tacatcagca gaagctcaga acaatccaga gaatgcagaa gaaatcacga
     1981 accaattgac agatgggcaa acattgacac atacccatca agcagatttg tctgaattcg
     2041 aaaactatac tggctatgct ttttatgctc gctcatctgc taattctcaa caagcgttca
     2101 ttgatagtat tgcctcaact gcacaaagtt tggcaagtgc caatgactta tatgcatcag
     2161 tcatgattgc tcaagccatc gttgaaagtg gctgggggaa cagtactttg gcttctgccc
     2221 ctaattacaa tctatttggg attaaaggaa gctataatgg ccaatctgtt acgatgccta
     2281 cttcggaata tgtgaatggg caatggatta cggtgaatgc cgcttttaga aaatatcctt
     2341 cgtacaaaga atcattgcaa gataacgtaa cggtattgaa gacgacttct tttcagccag
     2401 gcgtttacta ctattccggt gcatggaaaa gcaatacgaa ctcttacaaa gatgctacgg
     2461 cttggctaac aggtcgttat gccactgcgc ctaattatgg ttctacattg aacaatgtaa
     2521 tcgaaaccta taatctgaca cagtatgata cagcaccaac ttcaacttct ccgatgtatc
     2581 gtctgtataa ccgtcataca ggagaacatt tatatacatt gaatgccgga gaaaaaaatt
     2641 atctgccaac tgtaggctgg gaatatgaag ggattgcttg gcaagcacca aacagcggac
     2701 aacctgtcta ccgtttatac aatccttaca gtggagacca tcattacaca atggcacaat
     2761 ctgagatcaa ctctttaaca aaaatcggtt ggcgttatga aggtctaagt ttttattctg
     2821 gtggttccaa gccaatttat cgactattta atccaaatga aaaaactggt acccatcact
     2881 ataccttgag tgctaaggaa agagattggc taacaccgat gggctggaga tatgaaggaa
     2941 taggatttta tggttattaa aaagaggtag tgaaaaagct gccctgcatc aagtagtaag
     3001 aacagccaaa caaaaacaga ttttcatgtt ttttgtttgg ttgaacttat taccgcagat
     3061 gcaagttttt gaacaccgtt tactcaaaaa aggttgtgac aacatcttgt cataactttt
     3121 ttattactgt ttaagcatac tgatccaatt tctaatattc gacgggattc ttgcaaaagg
     3181 cgatttcgga aatttttggc ggtgtaaaat tatctactgc agaagagcta tcttctatag
     3241 tttgataaga atcaataatc ttggttttag cgttgtgctt tctttatcat tttttccgtt
     3301 cattctacaa aaaatttctt ttctctatac ttttctattg agagcgtttt ttttattaca
     3361 cagctgattc tttgactttt ttcttttttt attctattat tgacaagtca atcgttgacg
     3421 agtcaattat tagaaaggag gaaattatgt ctttagaaga gattagtcac cttttatatc
     3481 aaatcaaatt agccgatcaa aaaatcgtcc agatctttga agaaaatatc ggtataagtc
     3541 ttactagata cgagatcatg atgagactga aagaagaaaa tccattgcta caaagcagtt
     3601 tgcaagaaaa gttgcagatt gatcaaggag cagttactcg tcatctgaag gtattggaag
     3661 caaatggcta tgtttcgcgt cagagaaatc ctcagaacaa ccgggaagtg tatgtagcgc
     3721 taacagaaaa agcccagaag aaaatcaagc attgtgaaat ggaccataaa gatacaggtt
     3781 ccgtgctatc cccggctttc agcgatgtag aaatgaaaca atttctaaca cttttagata
     3841 aattgaatac ccaactttgc aatataacaa cagaaacgaa agaggttaat aataatggaa
     3901 caaaaaaatg atttttcaac aattatgact ggtagaagat ccgttcgcgt atatgatgaa
     3961 acatacaaaa tcccacacga agaaatgctg gaaatgatca aagaagcgac gactgcacct
     4021 tcttctgtca atatgaaacc atggcgcttt gtggtcgctg aaagcgagga agcaaaagaa
     4081 atcttgcgtc ctttgatccg tttcaatacg agacaaaatg atacttcttc tgcaatggtc
     4141 atgatttttg gcgatatgca atgttacgaa tatggcgaag aaatctacaa ccaagcttac
     4201 gaatcaggga agatgccaaa agaagtcaga gatcaacaat tagctgcgat cattccttat
     4261 tacaagagtt tttcaagaga agaaatgaac gatgtagtca aagtcgattc tagcttagcg
     4321 gctatgcaat tcatgttagt tgcgcgtgat catggctatg aaacaaatcc aattggtgga
     4381 ttcgaagcag atcagttggc agaggcattt ggtttggata aagaccgtta tgttccagtt
     4441 attattttat caataggaaa agcggtagaa gaaggttacg agtctgttcg tttagcacca
     4501 gaaaagatca ctatgttcga ataaaaggtt ttcagttact attcagtagc tattgctttt
     4561 tttgaatcgt gtgtggtaac ctaataaaaa aggaggtcgg aaaaatgact gcatcgatga
     4621 aattacgttt cgaacttttg aataacaact aaataaattc aaaagacatg tataaacaac
     4681 aatttcatgg atggagtatg tccatttttt cgaacgacag tagaataggt gaaagctatg
     4741 acagatttgg tgatttagtc atagttattt cgttcagagt aggaaaagat gtgatgcagt
     4801 ttttttgcaa acagattttt ctgctctttt ttagtatcga taattgttgt tttacatgaa
     4861 ataggagtgt aaaacatatg gaaaatttag cagtaaatat aacaaatcta caagtcagct
     4921 ttggcaacca gctagaacta tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag
     4981 ggatcatagg agaaaatggt gtcggtaaat cgactttgct caaactaata gccggtgaac
     5041 tttttcccga tcatgggaaa atccaaacag agatcacctt caactacctg cctcaattaa
     5101 cctatctcgc tgaagcaaag gacctaaatt tggaattagc cagtcatttc cagttaagac
     5161 tggaagaaac ttcagagcgg aaatggagcg gaggggaaga acgaaagatc gagttgatac
     5221 gtcttctttc ttcttatgaa caagggatgc ttctagacga gccaacaacc catctagata
     5281 gaaaaagtat tgatcgactg attgaagagc ttcgttatta ttatggtacg ctggtttttg
     5341 ttagtcatga tcgctatttt ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg
     5401 gagaaatccg agagttttcg gggaattata gtgcctatct cactcaaaag gaattggaga
     5461 aaaagactca gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat
     5521 cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa
     5581 agcaacaaat cagactggat cggttgtctt cctctaaaca aaaagacagt gtacaaaaag
     5641 ccatccaaaa gaatgcgaaa acattagaga gaagactcca aaaaatagga gaaacaacca
     5701 agccgcagca gatgaaacaa atccgttttc cagtaccaaa atctcttgaa ctccacagcc
     5761 gctatcccat catgggacaa aatgtccaat tggaaagaag tgggagaaca ttactggtaa
     5821 atggtgattt tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag
     5881 gtaagacaac cttattagaa catatccgca aacaaggaga aggaatcctt ctctctccga
     5941 aagtaagctt tcaagtatat cagcaaaagg gttatcaaat gacatctgaa gaatccatca
     6001 ttcgttttgt catgagacaa acagagtttt cagaatcgct tgtccgtagt ttgctgaatc
     6061 acttagggtt tgctcaggaa actttgacga aaccgttatg tacattaagt gggggagaag
     6121 cgacccgtct gacgattgct ttgcttttta ctaagccaag taatgtgttg ctgttagatg
     6181 aaccgactaa ttttattgat atggcaacga tcgaagcttt agagaagcta atgcaaatat
     6241 atccgggaac gattttgttt acttctcatg attcttactt tgtcgagcgt acggctgatg
     6301 aagtttatga aataaaaggg cagaaaataa aaaaagtact tacgagaaat ttttaaatgt
     6361 gctacgatga ttttcgtgac aaatttaaag gagtggtaca gattgcctac aaataaaaaa
     6421 gaaggaataa tttttacaac aatgatgtgt tttttgatgg tcttggggat gagcgcgtat
     6481 aatttatggc tgcatcaaga tcttcattta tcaagtttgt tgattggtct agtgcctgga
     6541 ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc
     6601 tttaaactac cagtaaatcc agaaagcaag gtccaaatga tcttaactat ttcatgcttg
     6661 atggttttag gaatggtgac atgtatgtct ttattcggtg tgatcatgga acaaggaata
     6721 cctgatcaat tattctctgc ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta
     6781 ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt
     6841 aatgaatgat ggaacactgt tatttgaaaa aaggccttgg catgcttttt gtcatagtct
     6901 cttgaatgca gttctagaaa aatagtgatc cgaacgatag gttttggcct gtcgttcgga
     6961 tcgctttttc ttttccaact gtattgctct tttattgaag ataatttgct cttcaataga
     7021 tatttctaga aaggttctgt ttatccctta ctcaaaaaac tgctgcattt gttctcttac
     7081 ttgctgacac cattggagtt cctgctgaca aagcataatt ctgcgctgga tcacaacata
     7141 acgtccaaat tcttgtttct tgtctagcga gaggtcattc aatttttttt gatttttttg
     7201 catgattttg ttcagctgtt gttctctagt gaatagcagt ccagtagctg ttgttttatc
     7261 gagcgtcgaa aaagcataga tttttgctag aaattcatct ttttgcgtag gagcgggagt
     7321 ttcctctttg atccattcct ctacaagagc acgtccttca tcagtcaagt ggtagatctt
     7381 tttttgagag tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt ctaacttggc
     7441 aagcgttgta tagatctgac tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa
     7501 ttcataccca gaacgtggtt tggataaaag cacgcataat aatatatagg ctaaagtatt
     7561 cataaaaatc ctacctctcg tcttattttc tttattatat cacagtaaag tttccacgtt
     7621 ttacatgtgc atattgacat gtaaaataaa tcatgtatga tgctaataga gagaaaggga
     7681 tggaaatatt ggagaatcaa atagatatac aagaacaaac aaatgaagaa acaaagggga
     7741 aaagttcagc gctagttgta gcagctacct gtatgatcgc ttttatggga gtgggtctag
     7801 tagacccaat cttaaaaacg attgctgcac agttaaacgc tacgccagct gaaacgaccc
     7861 tgttatttac tagttacatg ttagtcactg gtgtggtgat gctatttaca ggtttcttat
     7921 ctagtcggat cggtttgaaa aaaacattga tgacaggatt gtttatcatc gttgtattcg
     7981 ctggtcttgg cggattatct gggaatatcg gaatgctgat tgggttacgt gccggctggg
     8041 gtgtcgggaa tgctttattt gtctctactg ctttagcaac gatcgtcagt attttagttg
     8101 gtaacacaga aaaagcaatc atgatgtatg aagcagcact tggctgcgga atggctgttg
     8161 gccctctagt cggtggggtc ttgggcagtg gttcatggcg ttatccattt tttggggtag
     8221 catttttgat gttttgcggt tttatagccc tagcggtact tttacctgaa actgagaaac
     8281 caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt
     8341 cagttggact aatcgcattg ttatataatt tcggattctt tactttactt gcgtatgcgc
     8401 catttttatt gattggctat tctgaattag aagtcggttt tgtatttttt ggctggggtg
     8461 tgcttctggc tattgcttcg attttcgttg caccggctct tgagcggaag tttacgactt
     8521 atcggacaat tttagcagca tttgtcttat tcattatttg tttgctgtta ttaggagctg
     8581 gaacttctat gccagtcctt gtaccgatca gcattgtgtt agcaggattt ttccaaggaa
     8641 ttatcaatac actgttgacg acgattgcta tggaaatacc aggtctgcaa cgaaacgttg
     8701 cttcgtccag ctatagtttt gtccgctttt tcggtggagc tttagcacca tttattgcag
     8761 gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt gccattgttc
     8821 ttatcagtgt atgttgtatc atttatcacc gtaattattt tgctacaaat gaagaagaat
     8881 aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DAYYAG010000336         4826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.guided.163, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000336 DAYYAG010000000
VERSION     DAYYAG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.guided.163"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..196
                     /locus_tag="ACHARQ_003136"
     CDS             <1..196
                     /locus_tag="ACHARQ_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8411459.1"
                     /translation="QIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAK
                     DEVEDTADKSQQIVEEHLRK"
     gene            523..1020
                     /gene="dfrG"
                     /locus_tag="ACHARQ_003137"
     CDS             523..1020
                     /gene="dfrG"
                     /locus_tag="ACHARQ_003137"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8411460.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1092..3044)
                     /locus_tag="ACHARQ_003138"
     CDS             complement(1092..3044)
                     /locus_tag="ACHARQ_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8411461.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3051..3287)
                     /locus_tag="ACHARQ_003139"
     CDS             complement(3051..3287)
                     /locus_tag="ACHARQ_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411462.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3689..3979
                     /locus_tag="ACHARQ_003140"
     CDS             3689..3979
                     /locus_tag="ACHARQ_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411463.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4251..4600)
                     /locus_tag="ACHARQ_003141"
                     /pseudo
     CDS             complement(4251..4600)
                     /locus_tag="ACHARQ_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1587 a          792 c          836 g         1611 t
ORIGIN      
        1 acaaatcgca acactgacac aagaaaaaac aaatgtagaa actcagttaa cagatacaca
       61 aaagaaatta gacgaagcaa ctgctgaaaa ctcaagctgg aaacaatata ttcaaaagtt
      121 agaaaaagca aaagatgaag tggaagatac agctgataaa tctcagcaaa ttgttgaaga
      181 acatttgcgt aaataatctt tcttaacaag taaacctaaa ttagtaaaga agcaacagta
      241 atctatacta agtagttcag ttttggagta caaaatgaca aaatgtactt caaaatggac
      301 tacttttttc attttaactt acataccaaa ttacatacaa aatgatttat aaaatgcaat
      361 tgaaaatgat aattgaatga aaatttgtga ttttgtggta ttatgataat ataacttata
      421 gaaaaaattg cttattcaac taacgggcag gataatgaga aaaggttttt tgatagaaat
      481 aagaaattag atggtaaatt agttaaaaag gctgtgttat aaatgaaagt ttctttgatt
      541 gctgcgatgg ataagaatag agtgattggc aaagagaatg acattccttg gaggattccc
      601 aaggactggg aatatgttaa aaatactaca aagggacatc cgataatatt aggtaggaag
      661 aaccttgaat caatcggaag agccttacct gacagaagaa atattattct gacgagagat
      721 aaggggttta cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta
      781 tgtaaaaacg aagaagaaat ttttattttc ggaggagaac agatttataa tttgtttttc
      841 ccttatgttg agaaaatgta catcacaaaa atacatcatg aattcgaagg agatactttt
      901 tttccagaag tgaattatga ggaatggaat gaggtatttg cccaaaaagg gataaagaat
      961 gataaaaatc cgtataacta ctattttcat gtatatgaaa gaaaaaactt attgagttaa
     1021 cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac aagagcttgg gattactcca
     1081 agctcttttt gtcatttgaa ataaattata tctttccaat caaattgttt atctgtaatc
     1141 cctaatcttt gagcaggtgt caaaaccttt ttatccgctg atttaaaagg gcgacaaaag
     1201 ttgtagtaag ttcttagaat tgtaatcgca aattgagcat acttcggatt gaagttagcg
     1261 taaatatagc tcttcttatc accacgagct gttgttaatg gtctttcaag agatgatata
     1321 taacgtctta tctgttgaat aaagctattg gtcgaatggt cattaacatt taatagcatc
     1381 tttgcgattt cgtttggttc cagagcagat aaatccgttc tacaatcaac tgaataaaat
     1441 cctttatcct tagtggctaa tggatgtttg atagggttat cggcatattt gcgatatctt
     1501 tctccatctt tactaatagc ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga
     1561 taatgagatg ccaaagtccg tagaaacttt gttttgaatc ctctgatatc tccccaatcg
     1621 agcagttctg cttttgcttg tccgaattct tttaagcatt gttttctgct tttcgtcttg
     1681 ttaacttggc aaataaaatg atgtgcatcg ctcaacctta attctttcgt gaatactcta
     1741 taaaaggcgt ttctaataga gaagtcatca tcactaatca ttcgccattc gtcggcatta
     1801 accatttgtt taattaacca atagtgagca gttgtagtat aaggagcatc aacatttaaa
     1861 ccatcgacaa atctactcct attagtaatt ttccctaatt cgtgaagata agcattccta
     1921 ttttcgctat cgttagcgga tggttcttgt ggataataag accaatctaa cctatcattt
     1981 ttacgactaa aagtattgag atggtcttct ttgaattttc ttgtatcttc atttaattca
     2041 tccattgaaa tattccaatc ataggcaaca tcagaacgga acacatatct tgataagacc
     2101 tcagcagata caactatata cgtttgcata ttaaggtctt caaaccccgt atatttcttt
     2161 gaaccttgtc ctttcttacg gacgttattt aggtagtagt gcatcttgtc cgtattaagc
     2221 cacatctcgt taaatttctt tgtttgaagg ggttgtgtct catatcgttc taaaaactcc
     2281 aaacaacgcc tatacagcca ctccagctta tgatagtagg ttccaactcc aattcctaaa
     2341 atatcacaag tacgactgac tgaaaccctt ccaacaacca tcttagcgaa cattggaaga
     2401 atggtgttct tttgttggtg ataagtagtg gtttcttctc tttttggtag aacattagtg
     2461 aacttcttac acgccttaca ttgatagcgt tgagatttgc ccctactttt cccccttttg
     2521 taaaattgct tcggctcatt gaatggggtt gattcttctt ccgagcagcc ttctttatga
     2581 aattgataat ctggctcaac atcttggatg ctattgattt ctataaggcg tttaatttct
     2641 tcaacaacag accaattaga taaaggggtt actgtacagt tttgcgatac acctctgcca
     2701 ataggattgt cattgcagta cataccttta tgacctttat ctttggaact accagtcatt
     2761 ttatatcgac ttggtttccc ttttacatct ttgaatttgt gctgttcttt cccaaaatta
     2821 caacaaaacg gattgatgca gtaattatat tgaatgggat attcagaacc gttccattta
     2881 aaagacgttg gcaagaacaa cattttgtta taccttgttg caaatcgttt tggagttaat
     2941 aaatcatatt gtttagccct atcttttaat tcttcgtttg aaatcggaat tttaacatct
     3001 acaatttcat cttccttcgt ggctaatctt ttaagcttgg tcattgtcta tcaacccact
     3061 ctatctttga tacccatttt ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat
     3121 tcaataaact tcttatcgtc taaaatgttc aataagaatt catccactgc ttcgctttca
     3181 gtacgctctg catattcagc gtattgctta actatcaccc ttacttgctc tgatatttcc
     3241 caatccacag acttagcgtt cttatttttt ggctcaagaa attgcatagg aaaatgcacc
     3301 cctttttgct tagtaaattg taaccatttt aacacaccaa tttgcattcc tacaataatt
     3361 atttcacgtt cgcattttta tatacatttt aacttacatt ttgtggtgta aattaacaac
     3421 aaacgttgat ttaacaataa aaaacgagta tcaaattcag ttgcaatttg atactcattt
     3481 aggtttacat tttcactttt tgtactccaa aactgaacta cttaaatcta tactgctgcc
     3541 tctttttttt agtacatgtc cttcatcttc gcaggaagtg ccaatatgat tttaagaaag
     3601 gtaatagaca aaaagtcaaa aagataaaac agagtttctt ctttacaaag ggaatgtatg
     3661 ttcgtataat ggtattgagg tgaaggttat ggagaaaaaa tatggaacag tagataagat
     3721 ccgtactttg caagttagtg ccagaccgtt gatccgtttc agtttaaatg gggtcaattg
     3781 tttgattgct tcccatagtt tgaacttcct agctgaagta gacgaaggga tgaagttagt
     3841 cgttacaggt tattataata atagaaagca atttgttgtg agatcctatc atgtgttagg
     3901 gaaacccaaa attgtggtag aatatgagaa aagtctatac ccaagaaaaa aggtccgaca
     3961 agatgaaaga ataaactaga aaacaagcgg ctgagacaaa aatgtcctag ccgcttgttt
     4021 tggaaagatg gatgcagacg atgggatttg aacccacacg agcatacgct cacaagaacc
     4081 tcaatctata tataactaca tgtatttaag tatttgtgat gaatcactaa actttccaaa
     4141 ctcatttgag ggatattgct taaagattga acagatcaat tcgaagccct attagtattc
     4201 gctcaatggg gggatcagag gccataaaaa gagactagtc attactagtc tcaggtagtg
     4261 gaattgtagg attttaaacg ttcctggaga gtaccggtat gtaactcgaa gaggtgatta
     4321 tcatagtcgt aaaagtaaat tgaagaagcc tcgcctttca cacgagaacg tcctggttcg
     4381 acagttaaac ctaacatttg gatttttgag acaaacgaat ctatatcttg ttcgtcaatt
     4441 ttaaaagcaa tatgattgta tgtctttggc agtttattcg acgaattctg cattactgca
     4501 atccataggt ccttaactaa gaaaaatttt tctggatata atgaaaattt ttttgttgac
     4561 tatcgtagat ttcttttgca tcaaataagg tatttaacaa ttaagtattt aaaagataac
     4621 ttttaagtta tctttaagta atgcttaaag ctatgttata ctctattagg aaatataaga
     4681 aggagtataa aaatgaacaa acataataga aaaataacga ataacaaaga actgatcttg
     4741 ttactaatag ggatttgggg tatcttttta ctctttaata tgtatatgcc gactatgaga
     4801 gcggatgacg tagtttatgc aagtag
//
LOCUS       DAYYAG010000337         3795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.guided.193, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000337 DAYYAG010000000
VERSION     DAYYAG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.guided.193"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHARQ_003142"
     CDS             110..193
                     /locus_tag="ACHARQ_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8411464.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHARQ_003143"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHARQ_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8411465.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHARQ_003144"
     CDS             complement(1318..2181)
                     /locus_tag="ACHARQ_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8411466.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHARQ_003145"
     CDS             complement(2183..2455)
                     /locus_tag="ACHARQ_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8411467.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHARQ_003146"
     CDS             complement(2472..2681)
                     /locus_tag="ACHARQ_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8411468.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACHARQ_003147"
     CDS             complement(2759..3655)
                     /locus_tag="ACHARQ_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8411469.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1373 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccct
//
LOCUS       DAYYAG010000338         2823 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.guided.210, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000338 DAYYAG010000000
VERSION     DAYYAG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.guided.210"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..448
                     /locus_tag="ACHARQ_003148"
     CDS             <1..448
                     /locus_tag="ACHARQ_003148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8411470.1"
                     /translation="IGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSVLLTVS
                     PQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMVLSNVW
                     LNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            555..1793
                     /gene="mef(H)"
                     /locus_tag="ACHARQ_003149"
                     /gene_synonym="mefH"
     CDS             555..1793
                     /gene="mef(H)"
                     /locus_tag="ACHARQ_003149"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8411471.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2020..2439
                     /locus_tag="ACHARQ_003150"
     CDS             2020..2439
                     /locus_tag="ACHARQ_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8411472.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2444..2683
                     /locus_tag="ACHARQ_003151"
     CDS             2444..2683
                     /locus_tag="ACHARQ_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8411473.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT          918 a          390 c          522 g          993 t
ORIGIN      
        1 aattggaaat gaaaatgcag taatatttgc taaaattaga gataacgata gtttaaacgg
       61 tttagaaaag ttaagagaaa tatttaagac agcggtattc aattctaacc aaagcgtttt
      121 attgacagtt tctcctcaac tattaaacaa tcctcgtttt cttgctatgc aaatagaaca
      181 aatgtatgaa attgtagtac ctgaatttgt tgaacccgtt ttagaacaag gtatcaagga
      241 taagtctttg gagattgaaa attcacatga aattgcagaa gttatcatgg tattaagtaa
      301 tgtatggctc aacccattag ttgaaatgac ggataaagag ggtatggaaa aacgttgcaa
      361 tacattcaat gcccttttaa atggaatagg catagacaca ttattagacg aagaaacaat
      421 ttctgcattt atacagtttt gtaaataact gtatttgtta tccctatacg atagactgct
      481 ctataaaaga gcagtttatt taaaccatta tataaaccga cggtctgttt gattaaataa
      541 aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa tcgtgatttt atacttgttg
      601 ttataggaca gataatttct ttatttggaa acgctatcct acgatttgca ttaccgtact
      661 atttgttaaa agaaactggt tcatcagctt tgtttggtat tgctacagcc tgctcttttt
      721 tacctatggt tattttgtcg ctggtaggtg gtattattgc tgacaggaca aataaaagaa
      781 acattatggt catactagat tttgtaacag caatcataat tttagtattt tatttgtcat
      841 tgggaaaaat tcctatcata cctttgttta ttgttgcttt gatgttactt tatggtattt
      901 ctggtgcata tcaaccagcg gtacaagcaa gtattcctct attggtatca tcagataaac
      961 ttaccgtcgg taatgctata ataaatcaag taaatacttt agcaaacttt atcggaccag
     1021 ctataagcgg agtagttttt caattttatg gtatcaatcc cattttaatt gttagtatta
     1081 tatgttttac tttttcagcc attatggaaa tttttatcca tattccacac caaaaaagac
     1141 ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt aaaagatagt tatacctttt
     1201 tgaaaaagga gaaacccatt tttatatccg taatcttttt ggtttgcatt ttcaatttgg
     1261 ttttaagtgc tgttttaatt gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc
     1321 cagatacact atatggtttt tcacaatcag cattatcatt aggtggttta tgtggtggta
     1381 ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa cctttatacc ttgttactta
     1441 tttgttctgt ttctgtttta cttatgggaa tttcgctatt tttagaattg cctgcaatag
     1501 tttcctactg ggtagttaca gtgatgtgct ttgtggctat gggtgtatct actatgtttg
     1561 ttgttcaaat ttacactctt gttcagacac aaacaccacc gcaattagta ggaaagataa
     1621 tggctacgct ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg gtttacggta
     1681 ttttattcga tgtatttata caagaaacat gggttgttat gtttgtagct tctattgctt
     1741 caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa gttagagata taatacaagg
     1801 ggttaaatag tatgttaggc taaaaacaat atataaaaga aaatttgaaa tctgctaaaa
     1861 ggatagtgtt ataaaagaaa gagtaatatt gcagtattta aggctcgcac aaatatatgt
     1921 tttgtgtggg cttttttctt accatttttc tgtatttcta aagggtataa acggtattcc
     1981 gtttatatat ttcagcatta gcagaaaggg ggtgagatta tgaggacatc ttctttcgag
     2041 catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa
     2101 agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc
     2161 gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt
     2221 aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga
     2281 ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga
     2341 gaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga
     2401 ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa gaaatgagta aagattatgg
     2461 ctatccctct tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga
     2521 gattttgaag ttttatgacg gttatatttc taaattatgc ttgcgtcctt tttaccactc
     2581 tgaaagtggt aaaatcatta tgcaggttga tgaagaacta aaaggagaaa ttcatacaga
     2641 catgatgaaa gcaatattga aatttgaaat cggagtgaag taatcaactg tataaaaaaa
     2701 tcgggagata cacagtttcg tgctatctcc cttagtagtt ttacggtcat atcaaggctc
     2761 attcaatcgt ggtaaattcg tggtaagatg agtgtttttc tatacttcaa aatgcttgaa
     2821 tgt
//
LOCUS       DAYYAG010000339          886 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7077
            SAMEA115708787-rid22593983.guided.290, whole genome shotgun
            sequence.
ACCESSION   DAYYAG010000339 DAYYAG010000000
VERSION     DAYYAG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708787
            Sequence Read Archive: ERR13198507
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290335.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290335.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:19:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 32 of 80
            Pseudo Genes (incomplete)         :: 34 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708787-rid22593983.guided.290"
                     /isolate="VRE7077"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..821
                     /gene="aac(6')"
                     /locus_tag="ACHARQ_003152"
     CDS             273..821
                     /gene="aac(6')"
                     /locus_tag="ACHARQ_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8411474.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          316 a          129 c          185 g          256 t
ORIGIN      
        1 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
       61 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
      121 gaataaagtg tttctgtata attttttatt ttataagtaa gataaataat gaaagaatag
      181 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
      241 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
      301 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
      361 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
      421 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
      481 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
      541 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
      601 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
      661 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
      721 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
      781 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
      841 aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttactt
//
